Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029478.01.T01 | XP_003617013.1 | 88.69 | 725 | 3 | 3 | 1 | 701 | 1 | 670 | 0 | 1287 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048952.01 | MsG0580029478.01 | 0.823580 | 1.282910e-53 | 1.109077e-50 |
| MsG0180000001.01 | MsG0580029478.01 | 0.811716 | 6.090065e-51 | 3.797542e-48 |
| MsG0180000065.01 | MsG0580029478.01 | 0.802964 | 4.368873e-49 | 2.168934e-46 |
| MsG0180000091.01 | MsG0580029478.01 | 0.811019 | 8.629057e-51 | 5.283186e-48 |
| MsG0180000235.01 | MsG0580029478.01 | 0.816656 | 4.942374e-52 | 3.523099e-49 |
| MsG0180000470.01 | MsG0580029478.01 | 0.800233 | 1.586402e-48 | 7.348334e-46 |
| MsG0180000475.01 | MsG0580029478.01 | 0.801123 | 1.044540e-48 | 4.948984e-46 |
| MsG0180000555.01 | MsG0580029478.01 | 0.821258 | 4.443033e-53 | 3.596389e-50 |
| MsG0180000585.01 | MsG0580029478.01 | 0.828050 | 1.114987e-54 | 1.095824e-51 |
| MsG0180000604.01 | MsG0580029478.01 | 0.859449 | 4.103916e-63 | 1.084311e-59 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0080048839.01 | MsG0580029478.01 | PPI |
| MsG0580024477.01 | MsG0580029478.01 | PPI |
| MsG0880047754.01 | MsG0580029478.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029478.01.T01 | MTR_5g086790 | 88.690 | 725 | 3 | 3 | 1 | 701 | 1 | 670 | 0.0 | 1287 |
| MsG0580029478.01.T01 | MTR_5g087050 | 86.923 | 650 | 10 | 3 | 76 | 701 | 4 | 602 | 0.0 | 1127 |
| MsG0580029478.01.T01 | MTR_5g087240 | 77.444 | 665 | 67 | 6 | 63 | 698 | 1 | 611 | 0.0 | 946 |
| MsG0580029478.01.T01 | MTR_5g087250 | 70.784 | 510 | 76 | 4 | 192 | 701 | 71 | 507 | 0.0 | 703 |
| MsG0580029478.01.T01 | MTR_5g086970 | 79.462 | 409 | 59 | 2 | 238 | 622 | 1 | 408 | 0.0 | 662 |
| MsG0580029478.01.T01 | MTR_5g086940 | 93.146 | 321 | 6 | 1 | 302 | 622 | 6 | 310 | 0.0 | 618 |
| MsG0580029478.01.T01 | MTR_3g045010 | 96.196 | 184 | 7 | 0 | 396 | 579 | 1 | 184 | 2.69e-130 | 384 |
| MsG0580029478.01.T01 | MTR_5g086960 | 93.939 | 165 | 8 | 1 | 460 | 622 | 17 | 181 | 1.16e-105 | 319 |
| MsG0580029478.01.T01 | MTR_3g110405 | 32.114 | 246 | 137 | 8 | 381 | 622 | 43 | 262 | 6.81e-24 | 103 |
| MsG0580029478.01.T01 | MTR_4g074875 | 28.163 | 245 | 156 | 7 | 358 | 599 | 3 | 230 | 7.38e-23 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029478.01.T01 | AT5G20930 | 60.647 | 742 | 197 | 9 | 1 | 701 | 1 | 688 | 0.0 | 862 |
| MsG0580029478.01.T01 | AT2G45490 | 34.146 | 246 | 132 | 10 | 381 | 622 | 28 | 247 | 2.32e-24 | 103 |
| MsG0580029478.01.T01 | AT2G45490 | 34.146 | 246 | 132 | 10 | 381 | 622 | 28 | 247 | 4.04e-24 | 103 |
| MsG0580029478.01.T01 | AT2G25880 | 32.114 | 246 | 137 | 8 | 381 | 622 | 31 | 250 | 1.22e-22 | 99.4 |
| MsG0580029478.01.T01 | AT2G25880 | 32.114 | 246 | 137 | 8 | 381 | 622 | 44 | 263 | 1.84e-22 | 99.0 |
| MsG0580029478.01.T01 | AT3G53930 | 31.687 | 243 | 141 | 10 | 375 | 613 | 20 | 241 | 2.64e-22 | 102 |
| MsG0580029478.01.T01 | AT3G53930 | 31.687 | 243 | 141 | 10 | 375 | 613 | 20 | 241 | 2.68e-22 | 102 |
| MsG0580029478.01.T01 | AT1G66750 | 35.648 | 216 | 121 | 9 | 374 | 588 | 12 | 210 | 4.15e-22 | 99.0 |
| MsG0580029478.01.T01 | AT4G32830 | 31.707 | 246 | 138 | 8 | 381 | 622 | 37 | 256 | 4.67e-22 | 97.8 |
| MsG0580029478.01.T01 | AT2G25880 | 32.114 | 246 | 137 | 8 | 381 | 622 | 92 | 311 | 6.34e-22 | 98.2 |
Find 167 sgRNAs with CRISPR-Local
Find 551 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGCATTCGGGATGAAGA+TGG | 0.562115 | 5:+97468658 | MsG0580029478.01.T01:CDS |
| AAATTTGCCAGGAATTTATA+CGG | 0.332181 | 5:+97470965 | MsG0580029478.01.T01:intron |
| AACAATATGACGATGGACCA+AGG | 0.608478 | 5:-97469066 | None:intergenic |
| AACACCTATATTGCCTGAGA+AGG | 0.583381 | 5:+97469580 | MsG0580029478.01.T01:CDS |
| AACTGTCTTAACTGAGACCT+GGG | 0.558445 | 5:+97467282 | MsG0580029478.01.T01:CDS |
| AAGAAATCTGGTCGTGGAAG+AGG | 0.396651 | 5:+97465588 | MsG0580029478.01.T01:CDS |
| AAGACCAAAATCAGTCACTT+TGG | 0.528872 | 5:-97469723 | None:intergenic |
| AAGACCTACTGTTTCTAATG+AGG | 0.569301 | 5:+97470329 | MsG0580029478.01.T01:CDS |
| AAGATTGTGGAGAATGATGT+GGG | 0.679447 | 5:+97469749 | MsG0580029478.01.T01:CDS |
| AAGTTGGAAGCTCGAATGGT+GGG | 0.614592 | 5:+97462757 | MsG0580029478.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGCATATGTCAAGCAT+TGG | - | Chr5:97467534-97467553 | None:intergenic | 30.0% | |
| AAATATGAAGGGAAAGAAAG+AGG | - | Chr5:97467356-97467375 | None:intergenic | 30.0% | |
| AATGAAAGAAAGGCAATAAG+AGG | - | Chr5:97469913-97469932 | None:intergenic | 30.0% | |
| AATGTCATTGAGATATGTTG+GGG | - | Chr5:97466006-97466025 | None:intergenic | 30.0% | |
| ACAGCTTATTAACATGTCAT+AGG | - | Chr5:97469290-97469309 | None:intergenic | 30.0% | |
| ACTTCAACAATTGTAAGAAG+TGG | + | Chr5:97465441-97465460 | MsG0580029478.01.T01:intron | 30.0% | |
| AGAAGAAGCAAAGTTACATA+AGG | + | Chr5:97469012-97469031 | MsG0580029478.01.T01:CDS | 30.0% | |
| AGAATTTGTATAGGCTGTTA+AGG | + | Chr5:97471163-97471182 | MsG0580029478.01.T01:three_prime_UTR | 30.0% | |
| ATAAACGAACAATATGACGA+TGG | - | Chr5:97469076-97469095 | None:intergenic | 30.0% | |
| ATAATCTCAACTGTTGAAGA+AGG | + | Chr5:97470767-97470786 | MsG0580029478.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 97462407 | 97471428 | 97462407 | ID=MsG0580029478.01;Name=MsG0580029478.01 |
| Chr5 | mRNA | 97462407 | 97471428 | 97462407 | ID=MsG0580029478.01.T01;Parent=MsG0580029478.01;Name=MsG0580029478.01.T01;_AED=0.36;_eAED=0.40;_QI=140|0.75|0.82|1|0.93|0.88|17|357|701 |
| Chr5 | exon | 97462407 | 97462525 | 97462407 | ID=MsG0580029478.01.T01:exon:25055;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97462658 | 97462903 | 97462658 | ID=MsG0580029478.01.T01:exon:25056;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97465195 | 97465281 | 97465195 | ID=MsG0580029478.01.T01:exon:25057;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97465528 | 97465713 | 97465528 | ID=MsG0580029478.01.T01:exon:25058;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97465813 | 97465884 | 97465813 | ID=MsG0580029478.01.T01:exon:25059;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97466095 | 97466202 | 97466095 | ID=MsG0580029478.01.T01:exon:25060;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97466763 | 97466807 | 97466763 | ID=MsG0580029478.01.T01:exon:25061;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97467081 | 97467193 | 97467081 | ID=MsG0580029478.01.T01:exon:25062;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97467276 | 97467342 | 97467276 | ID=MsG0580029478.01.T01:exon:25063;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97468433 | 97468522 | 97468433 | ID=MsG0580029478.01.T01:exon:25064;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97468589 | 97468768 | 97468589 | ID=MsG0580029478.01.T01:exon:25065;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97468942 | 97469152 | 97468942 | ID=MsG0580029478.01.T01:exon:25066;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97469552 | 97469816 | 97469552 | ID=MsG0580029478.01.T01:exon:25067;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97469945 | 97469999 | 97469945 | ID=MsG0580029478.01.T01:exon:25068;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97470195 | 97470356 | 97470195 | ID=MsG0580029478.01.T01:exon:25069;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97470445 | 97470588 | 97470445 | ID=MsG0580029478.01.T01:exon:25070;Parent=MsG0580029478.01.T01 |
| Chr5 | exon | 97470976 | 97471428 | 97470976 | ID=MsG0580029478.01.T01:exon:25071;Parent=MsG0580029478.01.T01 |
| Chr5 | five_prime_UTR | 97462407 | 97462525 | 97462407 | ID=MsG0580029478.01.T01:five_prime_utr;Parent=MsG0580029478.01.T01 |
| Chr5 | five_prime_UTR | 97462658 | 97462678 | 97462658 | ID=MsG0580029478.01.T01:five_prime_utr;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97462679 | 97462903 | 97462679 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97465195 | 97465281 | 97465195 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97465528 | 97465713 | 97465528 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97465813 | 97465884 | 97465813 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97466095 | 97466202 | 97466095 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97466763 | 97466807 | 97466763 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97467081 | 97467193 | 97467081 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97467276 | 97467342 | 97467276 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97468433 | 97468522 | 97468433 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97468589 | 97468768 | 97468589 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97468942 | 97469152 | 97468942 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97469552 | 97469816 | 97469552 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97469945 | 97469999 | 97469945 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97470195 | 97470356 | 97470195 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97470445 | 97470588 | 97470445 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | CDS | 97470976 | 97471071 | 97470976 | ID=MsG0580029478.01.T01:cds;Parent=MsG0580029478.01.T01 |
| Chr5 | three_prime_UTR | 97471072 | 97471428 | 97471072 | ID=MsG0580029478.01.T01:three_prime_utr;Parent=MsG0580029478.01.T01 |
| Gene Sequence |
>MsG0580029478.01.T01 ATGTCGGATGATATGCTAGTGCATTTTTCGTCGAATTCTTCGAACCAATCGGATCAGTCTCTGCCTACAAAGATTGCCAAGTTGGAAGCTCGAATGGTGGGAAAAGGTTCTTCAACTGCTTCTCAGCAACAAGGTTGGCCTTCTGTTTCTTCTGCTGGGAAATTCAGTGGAGTGGCAGCTGAAGAAATGGTTGAGCCTTGCAGTTCAAGTGATTCTGATGATGATAATGGTGCGGATTTCTTGATTCAAGCCAACACACGTAAGCGCTTAAAACCTCACGATGATAACGATGATGTTTTTGAACGTGCTGAGGTTGTTACTGAAGGAAGACAAACTAGTTTGGAGACAAAAAATAATGTTGATGCAAATAGAAAGAAATCTGGTCGTGGAAGAGGGAGTTCTGGTTCAAGCAGAGGTCGTGGTTCCAAATCTAATGATCAGACCAGAACACAAATTTCTTCTTCAACAGTTTCGGCTTCAAATGGTCAGAATGAGAATAACTCATATTTTTGTTTGCAGGATGGTAGACTCAAAGATCCGTTTCGTAATGATAATGGTGCTTCATTAGAGGAGGAGATTGCATCTTTACGTGCAAAAGTTGTTGTGTTGGAGGAAGACTTACGTAAAGCAAAGCAAGAAGCCTCTCAGCATCAGAATATCTGTCCACAGTTAGAAAAGGAACTGAAGGAATTGAAAGATCTTGAAGAGCAAATGAAACCTAAGAGAACAAAAATAATATCCGATTTGCTGATATCAGTTTCAAAGGCTGAGAGACAAGAAGCAAGACTAAAAGTACGACAGGACTCATTGAGACTCGGAAATGTTGGTGTAATCAGAGCTGGAACTGTCTTAACTGAGACCTGGGAAGATGGTCAGGCGTTAAAGGATCTGAATGCCCAACTTGGTGATGGAATAGATGCAGAGGCTGGATTGCTGGAAGATATTCTCATTCACGAGGAAATCTATAAGTCTCGACTAGCCAGCATTAAACGGGAAGAAGAAATTGTTTTGCGAGAGAGGGACCGCTACGAACTAGAGAAGGGAAGACTAATTCGTGAAATGAAACGCATTCGGGATGAAGATGGTTCACGGTTCAACAATTTTCAGATTCTAAACCATCGTTACGCCCTTCTAAACCTTCTTGGAAAGGGAGGATTCAGCGAGGTGTACAAGGCTTTTGATTTGGTGGAGCATAGATATGTTGCATGCAAGCTTCATGGTTTAAATGCTCAATGGAGTGAAGAGAAGAAGCAAAGTTACATAAGGCATGCAATTCGGGAGTATAATATTCACAAGACCTTGGTCCATCGTCATATTGTTCGTTTATGGGACATCTTTGAGATTGATCCAAATACATTCTGCACTGTTCTAGAGTATTGCAGCGGGAAAGATCTTGATGCTGTTCTTAAAGCAACACCTATATTGCCTGAGAAGGAGGCTAGAGTCATTTTAGTTCAGATTTTCCAAGGCCTTATTTACATGAATAAAAGAGCACTGAAGATTATCCATTATGATTTAAAGCCTGGAAATGTTTTGTTTGATGAGCTCGGTATTGCCAAAGTGACTGATTTTGGTCTTAGCAAGATTGTGGAGAATGATGTGGGATCCCAGGGTATGGAACTTACATCCCAGGGGGCTGGAACGTATTGGTATTTGCCTCCTGAATGCTTTGAGCTCAGCAAGACGCCTCTTATATCATCCAAGGTTGATGTCTGGTCTGCTGGTATTTTGTTTTATCAAATGCTCTTCGGCAGACGTCCTTTCGGACATGACCAAACACAAGAGAGAATACTTCGTGAAGACACAATTATTAAGGCACGCAAGGTTGAATTTCCCTCAAGACCTACTGTTTCTAATGAGGCAAAGTGGACACAATCTTCACCTGTTTTAGGTTATATTATTGCACGAAAATCCGACTTTATTCTATGTGCAGGCGCAATTAGCCTTGACAATTTATCTGAATGTGCAAATTCTCTCCTCGGTATCACAATGCCCATTAAAATGTTAATGGAATTTATACGGCGGTGTCTAACATATAACCAGGCTGAGAGACCAGATGTATTGACCATTGCCCAAGATCCATATCTCACATACGTGAAGAAGTAG |
| Protein sequence |
>MsG0580029478.01.T01 MSDDMLVHFSSNSSNQSDQSLPTKIAKLEARMVGKGSSTASQQQGWPSVSSAGKFSGVAAEEMVEPCSSSDSDDDNGADFLIQANTRKRLKPHDDNDDVFERAEVVTEGRQTSLETKNNVDANRKKSGRGRGSSGSSRGRGSKSNDQTRTQISSSTVSASNGQNENNSYFCLQDGRLKDPFRNDNGASLEEEIASLRAKVVVLEEDLRKAKQEASQHQNICPQLEKELKELKDLEEQMKPKRTKIISDLLISVSKAERQEARLKVRQDSLRLGNVGVIRAGTVLTETWEDGQALKDLNAQLGDGIDAEAGLLEDILIHEEIYKSRLASIKREEEIVLRERDRYELEKGRLIREMKRIRDEDGSRFNNFQILNHRYALLNLLGKGGFSEVYKAFDLVEHRYVACKLHGLNAQWSEEKKQSYIRHAIREYNIHKTLVHRHIVRLWDIFEIDPNTFCTVLEYCSGKDLDAVLKATPILPEKEARVILVQIFQGLIYMNKRALKIIHYDLKPGNVLFDELGIAKVTDFGLSKIVENDVGSQGMELTSQGAGTYWYLPPECFELSKTPLISSKVDVWSAGILFYQMLFGRRPFGHDQTQERILREDTIIKARKVEFPSRPTVSNEAKWTQSSPVLGYIIARKSDFILCAGAISLDNLSECANSLLGITMPIKMLMEFIRRCLTYNQAERPDVLTIAQDPYLTYVKK* |
