AlfalfaGEDB | MsG0280009691.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009691.01

KEGG: K13508
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009691.01.T01	XP_039687130.1	94.958	238	12	0	1	238	1	238	3.70E-162	470

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009691.01.T01	Q9SHJ5	41.237	291	115	11	4	238	6	296	1.65E-54	188

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009691.01.T01	A0A396IMT2	94.958	238	12	0	1	238	1	238	1.77e-162	470

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047884.01	MsG0280009691.01	0.804810	1.806256e-49	9.403740e-47
MsG0080047885.01	MsG0280009691.01	0.802263	6.094304e-49	2.972060e-46
MsG0080048727.01	MsG0280009691.01	0.818819	1.606474e-52	1.215180e-49
MsG0080049002.01	MsG0280009691.01	0.810166	1.319097e-50	7.894749e-48
MsG0080049068.01	MsG0280009691.01	0.823411	1.405042e-53	1.208751e-50
MsG0180000114.01	MsG0280009691.01	0.800120	1.672931e-48	7.726914e-46
MsG0180000599.01	MsG0280009691.01	0.842465	2.543169e-58	3.859618e-55
MsG0180000782.01	MsG0280009691.01	0.818649	1.755250e-52	1.321486e-49
MsG0180000784.01	MsG0280009691.01	0.804806	1.809692e-49	9.420556e-47
MsG0180000843.01	MsG0280009691.01	0.800082	1.702950e-48	7.858086e-46
MsG0180000977.01	MsG0280009691.01	0.802946	4.407090e-49	2.186859e-46
MsG0180001064.01	MsG0280009691.01	0.820269	7.500916e-53	5.907321e-50
MsG0180001085.01	MsG0280009691.01	0.805301	1.425918e-49	7.517293e-47
MsG0180001100.01	MsG0280009691.01	0.862700	4.208384e-64	1.243724e-60
MsG0180001105.01	MsG0280009691.01	0.808993	2.357110e-50	1.367539e-47
MsG0180001610.01	MsG0280009691.01	0.801462	8.900630e-49	4.253189e-46
MsG0180002024.01	MsG0280009691.01	0.810678	1.022753e-50	6.204829e-48
MsG0180002399.01	MsG0280009691.01	0.836375	9.711493e-57	1.221555e-53
MsG0180002700.01	MsG0280009691.01	0.809777	1.600298e-50	9.481212e-48
MsG0180003353.01	MsG0280009691.01	0.813870	2.056367e-51	1.358716e-48
MsG0180003906.01	MsG0280009691.01	0.808679	2.751277e-50	1.582986e-47
MsG0180004103.01	MsG0280009691.01	0.844638	6.680018e-59	1.085216e-55
MsG0180004246.01	MsG0280009691.01	0.834056	3.737224e-56	4.384362e-53
MsG0180004448.01	MsG0280009691.01	0.810396	1.176793e-50	7.085345e-48
MsG0180004518.01	MsG0280009691.01	0.810793	9.659455e-51	5.878176e-48
MsG0180004541.01	MsG0280009691.01	0.800409	1.460602e-48	6.796220e-46
MsG0180004735.01	MsG0280009691.01	0.811541	6.649392e-51	4.127287e-48
MsG0180004934.01	MsG0280009691.01	0.824101	9.686813e-54	8.497910e-51
MsG0180005391.01	MsG0280009691.01	0.818308	2.097690e-52	1.564562e-49
MsG0180005803.01	MsG0280009691.01	0.808781	2.615958e-50	1.509156e-47
MsG0180005807.01	MsG0280009691.01	0.823367	1.439208e-53	1.236587e-50
MsG0180005829.01	MsG0280009691.01	0.856829	2.468154e-62	5.966910e-59
MsG0180006096.01	MsG0280009691.01	0.804299	2.308868e-49	1.186214e-46
MsG0180006136.01	MsG0280009691.01	0.805114	1.560813e-49	8.188997e-47
MsG0280006914.01	MsG0280009691.01	0.835998	1.210430e-56	1.505291e-53
MsG0280007118.01	MsG0280009691.01	0.804342	2.262277e-49	1.163550e-46
MsG0280007309.01	MsG0280009691.01	0.805251	1.460806e-49	7.691095e-47
MsG0280008709.01	MsG0280009691.01	0.829690	4.469900e-55	4.609030e-52
MsG0280008929.01	MsG0280009691.01	0.803301	3.721219e-49	1.863360e-46
MsG0280008932.01	MsG0280009691.01	0.811905	5.540469e-51	3.472123e-48
MsG0280008942.01	MsG0280009691.01	0.802283	6.039389e-49	2.946799e-46
MsG0280009256.01	MsG0280009691.01	0.842133	3.115359e-58	4.678389e-55
MsG0280009507.01	MsG0280009691.01	0.822178	2.722072e-53	2.261383e-50
MsG0280009691.01	MsG0280009695.01	0.832990	6.894595e-56	7.834861e-53
MsG0280009691.01	MsG0280009755.01	0.818727	1.685580e-52	1.271803e-49
MsG0280009691.01	MsG0280009756.01	0.848841	4.740655e-60	8.814596e-57
MsG0280009691.01	MsG0280009953.01	0.808724	2.690849e-50	1.550005e-47
MsG0280009691.01	MsG0280009960.01	0.800221	1.595713e-48	7.389196e-46
MsG0280009691.01	MsG0280010353.01	0.839426	1.595855e-57	2.203168e-54
MsG0280009691.01	MsG0280010517.01	0.819350	1.216144e-52	9.334826e-50
MsG0280009691.01	MsG0280010564.01	0.831145	1.970804e-55	2.120096e-52
MsG0280009691.01	MsG0280010916.01	0.801531	8.619054e-49	4.125771e-46
MsG0280009691.01	MsG0280010975.01	0.822613	2.157464e-53	1.815033e-50
MsG0280009691.01	MsG0280011073.01	0.801508	8.711921e-49	4.167880e-46
MsG0280009691.01	MsG0280011255.01	0.843623	1.250226e-58	1.967713e-55
MsG0280009691.01	MsG0280011401.01	0.819841	9.395355e-53	7.312065e-50
MsG0280009691.01	MsG0380012005.01	0.806058	9.890849e-50	5.316863e-47
MsG0280009691.01	MsG0380012011.01	0.840128	1.047517e-57	1.477965e-54
MsG0280009691.01	MsG0380012567.01	0.807218	5.628220e-50	3.116973e-47
MsG0280009691.01	MsG0380013342.01	0.806778	6.972352e-50	3.817504e-47
MsG0280009691.01	MsG0380014386.01	0.836259	1.038936e-56	1.302492e-53
MsG0280009691.01	MsG0380014517.01	0.803496	3.390459e-49	1.706378e-46
MsG0280009691.01	MsG0380014909.01	0.843202	1.620128e-58	2.515778e-55
MsG0280009691.01	MsG0380015372.01	0.852368	4.832809e-61	1.007605e-57
MsG0280009691.01	MsG0380015438.01	0.803744	3.011537e-49	1.525339e-46
MsG0280009691.01	MsG0380015784.01	0.801112	1.049870e-48	4.972881e-46
MsG0280009691.01	MsG0380015891.01	0.811394	7.155482e-51	4.424225e-48
MsG0280009691.01	MsG0380015896.01	0.824814	6.580937e-54	5.893126e-51
MsG0280009691.01	MsG0380016057.01	0.801991	6.934762e-49	3.358393e-46
MsG0280009691.01	MsG0380016284.01	0.802754	4.829424e-49	2.384854e-46
MsG0280009691.01	MsG0380016540.01	0.818657	1.748336e-52	1.316541e-49
MsG0280009691.01	MsG0380016771.01	0.803058	4.178537e-49	2.079337e-46
MsG0280009691.01	MsG0380017338.01	0.819018	1.447443e-52	1.101077e-49
MsG0280009691.01	MsG0380017423.01	0.803989	2.678651e-49	1.365363e-46
MsG0280009691.01	MsG0380017437.01	-0.818048	2.401341e-52	1.778221e-49
MsG0280009691.01	MsG0380017931.01	0.844187	8.829966e-59	1.414473e-55
MsG0280009691.01	MsG0380018037.01	0.834883	2.316529e-56	2.785838e-53
MsG0280009691.01	MsG0380018069.01	0.819061	1.415632e-52	1.078052e-49
MsG0280009691.01	MsG0480018584.01	0.812413	4.292789e-51	2.727762e-48
MsG0280009691.01	MsG0480018911.01	0.803448	3.470109e-49	1.744244e-46
MsG0280009691.01	MsG0480019313.01	0.806571	7.709408e-50	4.198736e-47
MsG0280009691.01	MsG0480019408.01	0.806403	8.368318e-50	4.537931e-47
MsG0280009691.01	MsG0480019501.01	0.821987	3.014779e-53	2.490988e-50
MsG0280009691.01	MsG0480019711.01	0.800071	1.711706e-48	7.896303e-46
MsG0280009691.01	MsG0480019971.01	0.815221	1.033147e-51	7.080278e-49
MsG0280009691.01	MsG0480020024.01	0.821538	3.828027e-53	3.123037e-50
MsG0280009691.01	MsG0480020229.01	0.826774	2.256106e-54	2.136113e-51
MsG0280009691.01	MsG0480020690.01	0.802569	5.270765e-49	2.590465e-46
MsG0280009691.01	MsG0480021168.01	0.810818	9.538051e-51	5.808211e-48
MsG0280009691.01	MsG0480021207.01	0.818442	1.955783e-52	1.464312e-49
MsG0280009691.01	MsG0480021248.01	0.818050	2.399720e-52	1.777082e-49
MsG0280009691.01	MsG0480021294.01	0.801875	7.325263e-49	3.537369e-46
MsG0280009691.01	MsG0480021336.01	0.808393	3.166934e-50	1.808693e-47
MsG0280009691.01	MsG0480021377.01	0.827892	1.216860e-54	1.190402e-51
MsG0280009691.01	MsG0480021406.01	0.814955	1.183589e-51	8.053110e-49
MsG0280009691.01	MsG0480022537.01	0.829461	5.082319e-55	5.205118e-52
MsG0280009691.01	MsG0480022624.01	0.861498	9.833172e-64	2.788182e-60
MsG0280009691.01	MsG0480022918.01	0.818707	1.702824e-52	1.284097e-49
MsG0280009691.01	MsG0480023190.01	0.823802	1.138093e-53	9.901582e-51
MsG0280009691.01	MsG0480023381.01	0.822516	2.271740e-53	1.905813e-50
MsG0280009691.01	MsG0480023565.01	0.800707	1.270456e-48	5.955767e-46
MsG0280009691.01	MsG0480023578.01	0.808352	3.230963e-50	1.843281e-47
MsG0280009691.01	MsG0480024030.01	0.802975	4.346368e-49	2.158283e-46
MsG0280009691.01	MsG0580024340.01	0.811957	5.397731e-51	3.387528e-48
MsG0280009691.01	MsG0580024717.01	0.851015	1.168754e-60	2.333223e-57
MsG0280009691.01	MsG0580024759.01	0.843013	1.819041e-58	2.807794e-55
MsG0280009691.01	MsG0580025625.01	0.810515	1.109427e-50	6.701050e-48
MsG0280009691.01	MsG0580025906.01	0.806646	7.434472e-50	4.056463e-47
MsG0280009691.01	MsG0580026173.01	0.801472	8.860973e-49	4.235346e-46
MsG0280009691.01	MsG0580026498.01	0.825542	4.429425e-54	4.049158e-51
MsG0280009691.01	MsG0580027394.01	0.842353	2.723181e-58	4.118266e-55
MsG0280009691.01	MsG0580027980.01	0.801154	1.029449e-48	4.881323e-46
MsG0280009691.01	MsG0580028024.01	0.827165	1.819223e-54	1.742342e-51
MsG0280009691.01	MsG0580028033.01	0.805436	1.336443e-49	7.070075e-47
MsG0280009691.01	MsG0580029414.01	0.822568	2.210092e-53	1.856846e-50
MsG0280009691.01	MsG0580029529.01	0.800107	1.683359e-48	7.772472e-46
MsG0280009691.01	MsG0580029734.01	0.815091	1.103909e-51	7.538589e-49
MsG0280009691.01	MsG0580029879.01	0.800777	1.229310e-48	5.773263e-46
MsG0280009691.01	MsG0580029912.01	0.810645	1.039746e-50	6.302189e-48
MsG0280009691.01	MsG0680030464.01	0.816172	6.343375e-52	4.462252e-49
MsG0280009691.01	MsG0680030514.01	0.840006	1.127143e-57	1.584441e-54
MsG0280009691.01	MsG0680030515.01	0.821479	3.950035e-53	3.217235e-50
MsG0280009691.01	MsG0680030516.01	0.807213	5.640702e-50	3.123486e-47
MsG0280009691.01	MsG0680030680.01	0.845613	3.640705e-59	6.100980e-56
MsG0280009691.01	MsG0680030858.01	0.846502	2.086947e-59	3.599828e-56
MsG0280009691.01	MsG0680031146.01	0.805053	1.607066e-49	8.418651e-47
MsG0280009691.01	MsG0680032006.01	0.810711	1.006367e-50	6.110753e-48
MsG0280009691.01	MsG0680032254.01	0.831239	1.868732e-55	2.015791e-52
MsG0280009691.01	MsG0680032830.01	0.831679	1.455971e-55	1.591425e-52
MsG0280009691.01	MsG0680032832.01	0.805528	1.278418e-49	6.778955e-47
MsG0280009691.01	MsG0680033377.01	0.819684	1.020473e-52	7.906742e-50
MsG0280009691.01	MsG0680033465.01	0.814405	1.566808e-51	1.050426e-48
MsG0280009691.01	MsG0680034766.01	0.806884	6.623409e-50	3.636382e-47
MsG0280009691.01	MsG0680035859.01	0.806661	7.380932e-50	4.028886e-47
MsG0280009691.01	MsG0780036517.01	0.820279	7.459937e-53	5.876709e-50
MsG0280009691.01	MsG0780036567.01	0.803520	3.352879e-49	1.688489e-46
MsG0280009691.01	MsG0780036792.01	0.824727	6.901144e-54	6.164552e-51
MsG0280009691.01	MsG0780037363.01	0.812754	3.616529e-51	2.318601e-48
MsG0280009691.01	MsG0780037701.01	0.839570	1.464476e-57	2.030480e-54
MsG0280009691.01	MsG0780038378.01	0.808126	3.608975e-50	2.046683e-47
MsG0280009691.01	MsG0780039161.01	0.806248	9.022400e-50	4.873578e-47
MsG0280009691.01	MsG0780039186.01	0.807504	4.895010e-50	2.731200e-47
MsG0280009691.01	MsG0780039975.01	0.823845	1.112083e-53	9.686835e-51
MsG0280009691.01	MsG0780040278.01	0.831118	2.000465e-55	2.150271e-52
MsG0280009691.01	MsG0780040343.01	0.814726	1.330328e-51	8.996105e-49
MsG0280009691.01	MsG0780040700.01	0.808264	3.373206e-50	1.920031e-47
MsG0280009691.01	MsG0780041091.01	0.806126	9.570608e-50	5.153571e-47
MsG0280009691.01	MsG0780041262.01	0.853989	1.659129e-61	3.651300e-58
MsG0280009691.01	MsG0780041302.01	0.818942	1.506608e-52	1.143697e-49
MsG0280009691.01	MsG0880042022.01	0.801525	8.640766e-49	4.135620e-46
MsG0280009691.01	MsG0880042086.01	0.800295	1.540969e-48	7.149468e-46
MsG0280009691.01	MsG0880042859.01	0.807903	4.026398e-50	2.269820e-47
MsG0280009691.01	MsG0880043249.01	0.800379	1.481932e-48	6.890040e-46
MsG0280009691.01	MsG0880043277.01	0.810591	1.067978e-50	6.463981e-48
MsG0280009691.01	MsG0880043278.01	0.825282	5.102480e-54	4.630390e-51
MsG0280009691.01	MsG0880044487.01	0.840424	8.767113e-58	1.248285e-54
MsG0280009691.01	MsG0880045668.01	0.815229	1.028651e-51	7.051267e-49
MsG0280009691.01	MsG0880045722.01	0.838009	3.708799e-57	4.903525e-54
MsG0280009691.01	MsG0880045992.01	0.821572	3.760777e-53	3.071053e-50
MsG0280009691.01	MsG0880046533.01	0.829500	4.972783e-55	5.098435e-52
MsG0280009691.01	MsG0880046736.01	0.807663	4.529275e-50	2.537420e-47
MsG0280009691.01	MsG0880046765.01	0.815879	7.373126e-52	5.144968e-49
MsG0280009691.01	MsG0880047394.01	0.830542	2.770087e-55	2.927240e-52
MsG0280009691.01	MsG0880047481.01	-0.811639	6.330440e-51	3.939145e-48
MsG0280009691.01	MsG0880047770.01	0.829579	4.756520e-55	4.888231e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009691.01.T01	MTR_3g448430	86.283	226	31	0	13	238	1	226	1.82e-143	412
MsG0280009691.01.T01	MTR_5g091660	64.035	228	82	0	11	238	16	243	4.54e-103	310
MsG0280009691.01.T01	MTR_4g415290	43.069	202	105	5	46	238	7	207	6.68e-44	155
MsG0280009691.01.T01	MTR_1g040500	40.099	202	113	3	44	238	4	204	2.75e-42	152
MsG0280009691.01.T01	MTR_7g067380	41.146	192	104	4	55	238	19	209	9.58e-38	139
MsG0280009691.01.T01	MTR_8g030620	37.379	206	116	5	45	238	9	213	2.69e-37	137
MsG0280009691.01.T01	MTR_5g061520	42.361	144	81	2	59	201	15	157	1.51e-31	122
MsG0280009691.01.T01	MTR_1g086650	36.364	220	122	7	26	238	33	241	2.31e-31	122
MsG0280009691.01.T01	MTR_5g080360	32.979	188	116	4	60	238	2	188	1.02e-27	111

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009691.01.T01	AT1G06520	41.237	291	115	11	4	238	6	296	1.68e-55	188
MsG0280009691.01.T01	AT2G38110	40.196	204	110	6	46	238	10	212	1.73e-40	146
MsG0280009691.01.T01	AT1G01610	38.235	204	113	6	45	238	9	209	1.94e-35	132
MsG0280009691.01.T01	AT4G01950	40.994	161	87	4	48	204	36	192	1.73e-32	125
MsG0280009691.01.T01	AT4G01950	40.994	161	87	4	48	204	64	220	2.96e-32	124
MsG0280009691.01.T01	AT3G11430	37.879	198	112	7	53	241	4	199	1.33e-31	122
MsG0280009691.01.T01	AT4G00400	38.235	204	114	5	44	238	8	208	2.71e-31	121
MsG0280009691.01.T01	AT5G06090	38.021	192	108	7	59	241	8	197	3.71e-30	118
MsG0280009691.01.T01	AT1G02390	36.879	141	88	1	59	199	50	189	8.59e-29	114
MsG0280009691.01.T01	AT3G11325	40.952	105	58	2	70	170	9	113	1.97e-15	75.9
MsG0280009691.01.T01	AT3G11325	40.952	105	58	2	70	170	6	110	2.10e-15	75.5

Find 40 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTCACTGGTTTGATTTCT+AGG	0.151271	2:+56570906	MsG0280009691.01.T01:CDS
AGTGCTCTCATGGATTATTT+TGG	0.183297	2:+56570950	MsG0280009691.01.T01:CDS
AGAATGAATGGTTCCTTAAT+AGG	0.296318	2:-56570535	None:intergenic
GGATATAGAAGGCCTGATTT+AGG	0.310254	2:+56570971	MsG0280009691.01.T01:CDS
AGAAGGCCTGATTTAGGAAT+TGG	0.322699	2:+56570977	MsG0280009691.01.T01:CDS
ACATTCATGATATCCTATTA+AGG	0.327821	2:+56570522	MsG0280009691.01.T01:CDS
CACTTGTGTGCCTAGAGTTA+TGG	0.347773	2:+56570811	MsG0280009691.01.T01:CDS
CATGGAGAATCTTAACCTTA+AGG	0.353285	2:+56570748	MsG0280009691.01.T01:CDS
GAATGTCACAAACAAGTGTT+TGG	0.370761	2:-56570505	None:intergenic
AAGGCAACTAGCATGAAATA+AGG	0.373236	2:-56570560	None:intergenic
TTAAAAGTATCTTTGGATAG+AGG	0.384557	2:+56571103	MsG0280009691.01.T01:CDS
TTCATGCTAGTTGCCTTTGA+AGG	0.401979	2:+56570566	MsG0280009691.01.T01:CDS
TAGGCATGGTTTGGTTGATA+AGG	0.411704	2:+56570925	MsG0280009691.01.T01:CDS
TATGGTGGAAGGGTTTCTTA+AGG	0.415468	2:+56570829	MsG0280009691.01.T01:CDS
AGATTCTCCATGTAAAACTT+TGG	0.427361	2:-56570737	None:intergenic
GTGCTTCCAATTCCTAAATC+AGG	0.432690	2:-56570983	None:intergenic
AAATCACAATTCAAAATACT+AGG	0.439229	2:-56570467	None:intergenic
TTTGATTTCTAGGCATGGTT+TGG	0.440519	2:+56570916	MsG0280009691.01.T01:CDS
CAATCACATGGTAAAGCCTT+AGG	0.442507	2:-56570349	None:intergenic
ACAAAACCTCACAAGCCTTA+AGG	0.479431	2:-56570763	None:intergenic
TGAAATAAGGAAAGAATGAA+TGG	0.486593	2:-56570547	None:intergenic
ACTGTTGGATGCTATTTCAC+TGG	0.492281	2:+56570893	MsG0280009691.01.T01:CDS
TAGGACATGAACAAAGTAAG+AGG	0.498321	2:-56570610	None:intergenic
ACTGGTTTGATTTCTAGGCA+TGG	0.501346	2:+56570911	MsG0280009691.01.T01:CDS
TGATAAGGATAGTGCTCTCA+TGG	0.519997	2:+56570940	MsG0280009691.01.T01:CDS
AAAATACTAGGAAAGGTTGA+AGG	0.524012	2:-56570455	None:intergenic
AATGTCACAAACAAGTGTTT+GGG	0.534593	2:-56570504	None:intergenic
GTGCCTAGAGTTATGGTGGA+AGG	0.542356	2:+56570818	MsG0280009691.01.T01:CDS
TCTTAACCTTAAGGCTTGTG+AGG	0.549494	2:+56570757	MsG0280009691.01.T01:CDS
TAAAATGCTACCACCTTCAA+AGG	0.551003	2:-56570579	None:intergenic
ACAATTCAAAATACTAGGAA+AGG	0.557117	2:-56570462	None:intergenic
ATGCTAGTTGCCTTTGAAGG+TGG	0.557422	2:+56570569	MsG0280009691.01.T01:CDS
GCTACTGAGTTACACACTGT+TGG	0.557540	2:+56570878	MsG0280009691.01.T01:CDS
TGCCTAGAGTTATGGTGGAA+GGG	0.568356	2:+56570819	MsG0280009691.01.T01:CDS
AGGACATGGAAATGATTGCA+AGG	0.571708	2:+56570705	MsG0280009691.01.T01:CDS
TGGACTTAAAATAAAGGACA+TGG	0.586187	2:+56570691	MsG0280009691.01.T01:CDS
GGACATGGAAATGATTGCAA+GGG	0.595734	2:+56570706	MsG0280009691.01.T01:CDS
AACCCTTCCACCATAACTCT+AGG	0.605896	2:-56570821	None:intergenic
TTGTGTGCCTAGAGTTATGG+TGG	0.640186	2:+56570814	MsG0280009691.01.T01:CDS
AACATGATTTAACAATCACA+TGG	0.695379	2:-56570361	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTTTTAAAAGTATCTT+TGG	+	Chr2:56571096-56571115	MsG0280009691.01.T01:CDS	10.0%
!!!	AGATACTTTTAAAAAATTTA+TGG	-	Chr2:56571095-56571114	None:intergenic	10.0%
!!!	ATTTTGAATTGTGATTTAAA+GGG	+	Chr2:56570473-56570492	MsG0280009691.01.T01:CDS	15.0%
!!!	TATTTTGAATTGTGATTTAA+AGG	+	Chr2:56570472-56570491	MsG0280009691.01.T01:CDS	15.0%
!!!	TTTTAGCTATATTATATTCT+TGG	+	Chr2:56571065-56571084	MsG0280009691.01.T01:CDS	15.0%
!!	AAATCACAATTCAAAATACT+AGG	-	Chr2:56570470-56570489	None:intergenic	20.0%
!!!	TCATTTTATCTCTTATTGTA+AGG	+	Chr2:56571021-56571040	MsG0280009691.01.T01:CDS	20.0%
!!!	TTTTGAATTGTGATTTAAAG+GGG	+	Chr2:56570474-56570493	MsG0280009691.01.T01:CDS	20.0%
!!!	TTTTTGTGGACTTAAAATAA+AGG	+	Chr2:56570685-56570704	MsG0280009691.01.T01:CDS	20.0%
!	AACATGATTTAACAATCACA+TGG	-	Chr2:56570364-56570383	None:intergenic	25.0%
!	ACAATTCAAAATACTAGGAA+AGG	-	Chr2:56570465-56570484	None:intergenic	25.0%
!	ACATTCATGATATCCTATTA+AGG	+	Chr2:56570522-56570541	MsG0280009691.01.T01:CDS	25.0%
!	TGAAATAAGGAAAGAATGAA+TGG	-	Chr2:56570550-56570569	None:intergenic	25.0%
!!	AGTTTTACCAAAGTTTTACA+TGG	+	Chr2:56570730-56570749	MsG0280009691.01.T01:CDS	25.0%
!!!	ATGATCTTCATTACTTTTTG+TGG	+	Chr2:56570671-56570690	MsG0280009691.01.T01:CDS	25.0%
!!!	TGGATTATTTTGGATATAGA+AGG	+	Chr2:56570960-56570979	MsG0280009691.01.T01:CDS	25.0%
!!!	TTAAAAGTATCTTTGGATAG+AGG	+	Chr2:56571103-56571122	MsG0280009691.01.T01:CDS	25.0%
	AAAATACTAGGAAAGGTTGA+AGG	-	Chr2:56570458-56570477	None:intergenic	30.0%
	AATGTCACAAACAAGTGTTT+GGG	-	Chr2:56570507-56570526	None:intergenic	30.0%
	AGAATGAATGGTTCCTTAAT+AGG	-	Chr2:56570538-56570557	None:intergenic	30.0%
	AGATTCTCCATGTAAAACTT+TGG	-	Chr2:56570740-56570759	None:intergenic	30.0%
	TGGACTTAAAATAAAGGACA+TGG	+	Chr2:56570691-56570710	MsG0280009691.01.T01:CDS	30.0%
!	TAGCTCAAAAAGCATAACAT+AGG	-	Chr2:56570632-56570651	None:intergenic	30.0%
!	TGTTTCTTTTAGTACTCCTA+AGG	+	Chr2:56570333-56570352	MsG0280009691.01.T01:CDS	30.0%
!!	ATTTCACTGGTTTGATTTCT+AGG	+	Chr2:56570906-56570925	MsG0280009691.01.T01:CDS	30.0%
	AAGGCAACTAGCATGAAATA+AGG	-	Chr2:56570563-56570582	None:intergenic	35.0%
	CATGGAGAATCTTAACCTTA+AGG	+	Chr2:56570748-56570767	MsG0280009691.01.T01:CDS	35.0%
	GAATGTCACAAACAAGTGTT+TGG	-	Chr2:56570508-56570527	None:intergenic	35.0%
	TAAAATGCTACCACCTTCAA+AGG	-	Chr2:56570582-56570601	None:intergenic	35.0%
	TAGGACATGAACAAAGTAAG+AGG	-	Chr2:56570613-56570632	None:intergenic	35.0%
!	TTTGATTTCTAGGCATGGTT+TGG	+	Chr2:56570916-56570935	MsG0280009691.01.T01:CDS	35.0%
!!	AGTGCTCTCATGGATTATTT+TGG	+	Chr2:56570950-56570969	MsG0280009691.01.T01:CDS	35.0%
	ACAAAACCTCACAAGCCTTA+AGG	-	Chr2:56570766-56570785	None:intergenic	40.0%
	AGAAGGCCTGATTTAGGAAT+TGG	+	Chr2:56570977-56570996	MsG0280009691.01.T01:CDS	40.0%
	AGGACATGGAAATGATTGCA+AGG	+	Chr2:56570705-56570724	MsG0280009691.01.T01:CDS	40.0%
	CAATCACATGGTAAAGCCTT+AGG	-	Chr2:56570352-56570371	None:intergenic	40.0%
	GGACATGGAAATGATTGCAA+GGG	+	Chr2:56570706-56570725	MsG0280009691.01.T01:CDS	40.0%
	GGATATAGAAGGCCTGATTT+AGG	+	Chr2:56570971-56570990	MsG0280009691.01.T01:CDS	40.0%
	GTGCTTCCAATTCCTAAATC+AGG	-	Chr2:56570986-56571005	None:intergenic	40.0%
	TCTTAACCTTAAGGCTTGTG+AGG	+	Chr2:56570757-56570776	MsG0280009691.01.T01:CDS	40.0%
	TGATAAGGATAGTGCTCTCA+TGG	+	Chr2:56570940-56570959	MsG0280009691.01.T01:CDS	40.0%
	TTCATGCTAGTTGCCTTTGA+AGG	+	Chr2:56570566-56570585	MsG0280009691.01.T01:CDS	40.0%
!	TATGGTGGAAGGGTTTCTTA+AGG	+	Chr2:56570829-56570848	MsG0280009691.01.T01:CDS	40.0%
!!	ACTGGTTTGATTTCTAGGCA+TGG	+	Chr2:56570911-56570930	MsG0280009691.01.T01:CDS	40.0%
!!	ACTGTTGGATGCTATTTCAC+TGG	+	Chr2:56570893-56570912	MsG0280009691.01.T01:CDS	40.0%
!!	GTGAGGTTTTGTCTTCAGTA+GGG	+	Chr2:56570774-56570793	MsG0280009691.01.T01:CDS	40.0%
!!	TAGGCATGGTTTGGTTGATA+AGG	+	Chr2:56570925-56570944	MsG0280009691.01.T01:CDS	40.0%
!!	TGTGAGGTTTTGTCTTCAGT+AGG	+	Chr2:56570773-56570792	MsG0280009691.01.T01:CDS	40.0%
	AACCCTTCCACCATAACTCT+AGG	-	Chr2:56570824-56570843	None:intergenic	45.0%
	CACTTGTGTGCCTAGAGTTA+TGG	+	Chr2:56570811-56570830	MsG0280009691.01.T01:CDS	45.0%
	GCTACTGAGTTACACACTGT+TGG	+	Chr2:56570878-56570897	MsG0280009691.01.T01:CDS	45.0%
	TTGTGTGCCTAGAGTTATGG+TGG	+	Chr2:56570814-56570833	MsG0280009691.01.T01:CDS	45.0%
!	ATGCTAGTTGCCTTTGAAGG+TGG	+	Chr2:56570569-56570588	MsG0280009691.01.T01:CDS	45.0%
!	TGCCTAGAGTTATGGTGGAA+GGG	+	Chr2:56570819-56570838	MsG0280009691.01.T01:CDS	45.0%
!	GTGCCTAGAGTTATGGTGGA+AGG	+	Chr2:56570818-56570837	MsG0280009691.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	56570329	56571132	56570329	ID=MsG0280009691.01;Name=MsG0280009691.01
Chr2	mRNA	56570329	56571132	56570329	ID=MsG0280009691.01.T01;Parent=MsG0280009691.01;Name=MsG0280009691.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|267
Chr2	exon	56570329	56571132	56570329	ID=MsG0280009691.01.T01:exon:18568;Parent=MsG0280009691.01.T01
Chr2	CDS	56570329	56571132	56570329	ID=MsG0280009691.01.T01:cds;Parent=MsG0280009691.01.T01

Gene Sequence

>MsG0280009691.01.T01

ATGGTGTTTCTTTTAGTACTCCTAAGGCTTTACCATGTGATTGTTAAATCATGTTATGAAGCTGCAAAGAAATTGAAAAGTCACAGCTTTAATTTCATTGATCTATCATCTAAACAACTTCATCAACCTTCAACCTTTCCTAGTATTTTGAATTGTGATTTAAAGGGGAGAGAGTCCCAAACACTTGTTTGTGACATTCATGATATCCTATTAAGGAACCATTCATTCTTTCCTTATTTCATGCTAGTTGCCTTTGAAGGTGGTAGCATTTTAAGAGCTTTCCTCTTACTTTGTTCATGTCCTATGTTATGCTTTTTGAGCTATGAACATAAGCTTAAAGTCATGATCTTCATTACTTTTTGTGGACTTAAAATAAAGGACATGGAAATGATTGCAAGGGTAGTTTTACCAAAGTTTTACATGGAGAATCTTAACCTTAAGGCTTGTGAGGTTTTGTCTTCAGTAGGGTCAAGAATTTTCTTCACTTGTGTGCCTAGAGTTATGGTGGAAGGGTTTCTTAAGGAATATTTGAATGTTGATGATGTTGTAGCTACTGAGTTACACACTGTTGGATGCTATTTCACTGGTTTGATTTCTAGGCATGGTTTGGTTGATAAGGATAGTGCTCTCATGGATTATTTTGGATATAGAAGGCCTGATTTAGGAATTGGAAGCACATGTGTCAATGATCATCATTTTATCTCTTATTGTAAGGTATATGTGAAGTTAGTTACTCTTTTAGCTATATTATATTCTTGGTATTCCATAAATTTTTTAAAAGTATCTTTGGATAGAGGAAAATGA

Protein sequence

>MsG0280009691.01.T01

MVFLLVLLRLYHVIVKSCYEAAKKLKSHSFNFIDLSSKQLHQPSTFPSILNCDLKGRESQTLVCDIHDILLRNHSFFPYFMLVAFEGGSILRAFLLLCSCPMLCFLSYEHKLKVMIFITFCGLKIKDMEMIARVVLPKFYMENLNLKACEVLSSVGSRIFFTCVPRVMVEGFLKEYLNVDDVVATELHTVGCYFTGLISRHGLVDKDSALMDYFGYRRPDLGIGSTCVNDHHFISYCKVYVKLVTLLAILYSWYSINFLKVSLDRGK*