AlfalfaGEDB | MsG0280010022.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010022.01

KEGG: K03846
GO: GO:0016757
PFAM: PF03901

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010022.01.T01	AES66637.1	84.021	194	6	1	8	176	91	284	6.90E-97	305
MsG0280010022.01.T01	AES66637.1	90.278	72	7	0	162	233	424	495	4.82E-37	148

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010022.01.T01	Q9FZ49	65.464	194	42	1	8	176	94	287	2.10E-75	242

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010022.01.T01	G7IH72	84.021	194	6	1	8	176	91	284	3.30e-97	305

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0280010022.01	0.834557	2.797342e-56	3.331791e-53
MsG0080048058.01	MsG0280010022.01	0.851359	9.345192e-61	1.886895e-57
MsG0080048383.01	MsG0280010022.01	0.808165	3.541645e-50	2.010617e-47
MsG0180000001.01	MsG0280010022.01	0.869357	3.291083e-66	1.231647e-62
MsG0180000142.01	MsG0280010022.01	0.811456	6.936806e-51	4.296149e-48
MsG0180000144.01	MsG0280010022.01	0.829732	4.365869e-55	4.507394e-52
MsG0180000295.01	MsG0280010022.01	0.803162	3.977103e-49	1.984465e-46
MsG0180000364.01	MsG0280010022.01	0.803668	3.124053e-49	1.579147e-46
MsG0180000821.01	MsG0280010022.01	0.801010	1.101778e-48	5.205532e-46
MsG0180000836.01	MsG0280010022.01	0.805390	1.366185e-49	7.218749e-47
MsG0180001117.01	MsG0280010022.01	0.805087	1.580983e-49	8.289229e-47
MsG0180001173.01	MsG0280010022.01	0.859746	3.341359e-63	8.918908e-60
MsG0180001237.01	MsG0280010022.01	0.858729	6.744729e-63	1.738418e-59
MsG0180001294.01	MsG0280010022.01	0.828562	8.389527e-55	8.369147e-52
MsG0180001566.01	MsG0280010022.01	0.838579	2.644874e-57	3.557911e-54
MsG0180001911.01	MsG0280010022.01	0.810911	9.110461e-51	5.561675e-48
MsG0180003181.01	MsG0280010022.01	0.835351	1.765103e-56	2.153034e-53
MsG0180003303.01	MsG0280010022.01	0.855193	7.432602e-62	1.701882e-58
MsG0180003444.01	MsG0280010022.01	0.828613	8.157228e-55	8.149287e-52
MsG0180003586.01	MsG0280010022.01	0.839487	1.539208e-57	2.128829e-54
MsG0180004160.01	MsG0280010022.01	0.815423	9.316638e-52	6.420740e-49
MsG0180004272.01	MsG0280010022.01	0.803158	3.983748e-49	1.987591e-46
MsG0180004437.01	MsG0280010022.01	0.825126	5.554602e-54	5.018314e-51
MsG0180004598.01	MsG0280010022.01	0.821767	3.388469e-53	2.782441e-50
MsG0180004645.01	MsG0280010022.01	0.801059	1.076281e-48	5.091362e-46
MsG0180004754.01	MsG0280010022.01	0.815400	9.426442e-52	6.492247e-49
MsG0180004989.01	MsG0280010022.01	0.839769	1.299898e-57	1.813363e-54
MsG0180004991.01	MsG0280010022.01	0.834275	3.293859e-56	3.889975e-53
MsG0180005049.01	MsG0280010022.01	0.827763	1.307526e-54	1.274198e-51
MsG0180005443.01	MsG0280010022.01	0.826471	2.664199e-54	2.500806e-51
MsG0180005451.01	MsG0280010022.01	0.840359	9.116670e-58	1.295430e-54
MsG0180005493.01	MsG0280010022.01	0.827529	1.487834e-54	1.440099e-51
MsG0180005504.01	MsG0280010022.01	0.804375	2.225977e-49	1.145896e-46
MsG0180005765.01	MsG0280010022.01	0.804225	2.392185e-49	1.226642e-46
MsG0180005985.01	MsG0280010022.01	0.819698	1.012990e-52	7.852071e-50
MsG0180006023.01	MsG0280010022.01	0.831808	1.353290e-55	1.484933e-52
MsG0180006225.01	MsG0280010022.01	0.800566	1.357234e-48	6.340108e-46
MsG0180006235.01	MsG0280010022.01	0.811184	7.947093e-51	4.886457e-48
MsG0280006305.01	MsG0280010022.01	0.818451	1.946638e-52	1.457828e-49
MsG0280006367.01	MsG0280010022.01	0.823612	1.261384e-53	1.091443e-50
MsG0280006368.01	MsG0280010022.01	0.812135	4.938131e-51	3.114157e-48
MsG0280007048.01	MsG0280010022.01	0.815583	8.582059e-52	5.940476e-49
MsG0280007609.01	MsG0280010022.01	0.826806	2.216423e-54	2.100508e-51
MsG0280007764.01	MsG0280010022.01	0.816371	5.723810e-52	4.048420e-49
MsG0280007790.01	MsG0280010022.01	0.876905	9.629011e-69	4.750101e-65
MsG0280008614.01	MsG0280010022.01	0.880259	6.362629e-70	3.560045e-66
MsG0280008885.01	MsG0280010022.01	0.822474	2.323641e-53	1.946950e-50
MsG0280008909.01	MsG0280010022.01	0.825025	5.868119e-54	5.286703e-51
MsG0280010002.01	MsG0280010022.01	0.866243	3.286940e-65	1.100250e-61
MsG0280010022.01	MsG0280010109.01	0.809965	1.457442e-50	8.677331e-48
MsG0280010022.01	MsG0280010735.01	0.826985	2.008310e-54	1.913372e-51
MsG0280010022.01	MsG0280011075.01	0.812984	3.219825e-51	2.076868e-48
MsG0280010022.01	MsG0280011336.01	0.830545	2.765439e-55	2.922551e-52
MsG0280010022.01	MsG0380013548.01	0.818826	1.600805e-52	1.211149e-49
MsG0280010022.01	MsG0380014489.01	0.838779	2.347282e-57	3.176842e-54
MsG0280010022.01	MsG0380014541.01	0.805949	1.042697e-49	5.589388e-47
MsG0280010022.01	MsG0380014576.01	0.815957	7.084763e-52	4.954381e-49
MsG0280010022.01	MsG0380014579.01	0.811168	8.012453e-51	4.924409e-48
MsG0280010022.01	MsG0380014778.01	0.810111	1.356066e-50	8.104674e-48
MsG0280010022.01	MsG0380015436.01	0.802530	5.371147e-49	2.637116e-46
MsG0280010022.01	MsG0380015702.01	0.801341	9.426577e-49	4.490771e-46
MsG0280010022.01	MsG0380015793.01	-0.802396	5.723983e-49	2.800973e-46
MsG0280010022.01	MsG0380015831.01	0.843764	1.146641e-58	1.812381e-55
MsG0280010022.01	MsG0380015944.01	0.800580	1.348249e-48	6.300510e-46
MsG0280010022.01	MsG0380016483.01	0.828821	7.263290e-55	7.300085e-52
MsG0280010022.01	MsG0380016765.01	0.803433	3.493977e-49	1.755583e-46
MsG0280010022.01	MsG0380016882.01	0.820844	5.534377e-53	4.428812e-50
MsG0280010022.01	MsG0380016985.01	0.849655	2.813140e-60	5.370650e-57
MsG0280010022.01	MsG0380016994.01	0.808809	2.580658e-50	1.489883e-47
MsG0280010022.01	MsG0380017195.01	0.807428	5.079748e-50	2.828639e-47
MsG0280010022.01	MsG0380017431.01	0.848792	4.890691e-60	9.079165e-57
MsG0280010022.01	MsG0380017719.01	0.829427	5.179460e-55	5.299417e-52
MsG0280010022.01	MsG0380017797.01	0.804640	1.960199e-49	1.015955e-46
MsG0280010022.01	MsG0380017836.01	0.822702	2.056174e-53	1.734060e-50
MsG0280010022.01	MsG0480018256.01	0.815529	8.824129e-52	6.099057e-49
MsG0280010022.01	MsG0480018462.01	0.813890	2.035841e-51	1.345895e-48
MsG0280010022.01	MsG0480018641.01	0.811085	8.352561e-51	5.122462e-48
MsG0280010022.01	MsG0480018846.01	0.842463	2.547068e-58	3.865243e-55
MsG0280010022.01	MsG0480019170.01	0.826006	3.437512e-54	3.184437e-51
MsG0280010022.01	MsG0480020242.01	0.826922	2.079083e-54	1.977259e-51
MsG0280010022.01	MsG0480020331.01	0.824707	6.974512e-54	6.226734e-51
MsG0280010022.01	MsG0480020591.01	0.810750	9.866871e-51	5.997611e-48
MsG0280010022.01	MsG0480020780.01	0.804061	2.588256e-49	1.321713e-46
MsG0280010022.01	MsG0480020791.01	0.809227	2.099761e-50	1.225962e-47
MsG0280010022.01	MsG0480020917.01	0.812837	3.467053e-51	2.227565e-48
MsG0280010022.01	MsG0480021772.01	0.819502	1.122805e-52	8.656086e-50
MsG0280010022.01	MsG0480021900.01	0.802746	4.845761e-49	2.392492e-46
MsG0280010022.01	MsG0480022094.01	0.856816	2.490960e-62	6.018748e-59
MsG0280010022.01	MsG0480022115.01	0.838123	3.465761e-57	4.597325e-54
MsG0280010022.01	MsG0480022228.01	0.871634	5.886043e-67	2.391428e-63
MsG0280010022.01	MsG0480022464.01	0.824257	8.902787e-54	7.844619e-51
MsG0280010022.01	MsG0480022597.01	0.803457	3.454781e-49	1.736949e-46
MsG0280010022.01	MsG0480022598.01	0.812428	4.259827e-51	2.707951e-48
MsG0280010022.01	MsG0480022620.01	0.811855	5.682857e-51	3.556582e-48
MsG0280010022.01	MsG0480022678.01	0.805498	1.296456e-49	6.869518e-47
MsG0280010022.01	MsG0480022717.01	0.835447	1.669305e-56	2.041817e-53
MsG0280010022.01	MsG0480022719.01	0.832753	7.896958e-56	8.909749e-53
MsG0280010022.01	MsG0480023053.01	0.818899	1.540382e-52	1.167892e-49
MsG0280010022.01	MsG0480023240.01	0.824802	6.625274e-54	5.930891e-51
MsG0280010022.01	MsG0480023447.01	0.805954	1.040152e-49	5.576506e-47
MsG0280010022.01	MsG0480023500.01	0.861094	1.306185e-63	3.652001e-60
MsG0280010022.01	MsG0480023620.01	0.803622	3.192594e-49	1.611967e-46
MsG0280010022.01	MsG0480023718.01	0.817978	2.490950e-52	1.841024e-49
MsG0280010022.01	MsG0580024157.01	0.826026	3.399745e-54	3.151181e-51
MsG0280010022.01	MsG0580024618.01	0.845579	3.718038e-59	6.224097e-56
MsG0280010022.01	MsG0580024721.01	0.811694	6.158691e-51	3.837905e-48
MsG0280010022.01	MsG0580024819.01	0.836461	9.229984e-57	1.164008e-53
MsG0280010022.01	MsG0580024960.01	0.803565	3.280363e-49	1.653975e-46
MsG0280010022.01	MsG0580025072.01	0.813556	2.411396e-51	1.579792e-48
MsG0280010022.01	MsG0580025352.01	0.821441	4.032106e-53	3.280532e-50
MsG0280010022.01	MsG0580025900.01	0.804010	2.652695e-49	1.352787e-46
MsG0280010022.01	MsG0580027628.01	0.801692	7.988022e-49	3.839195e-46
MsG0280010022.01	MsG0580028060.01	0.843514	1.337330e-58	2.097366e-55
MsG0280010022.01	MsG0580029148.01	0.820220	7.695768e-53	6.052495e-50
MsG0280010022.01	MsG0580029371.01	0.855702	5.284018e-62	1.230765e-58
MsG0280010022.01	MsG0580029588.01	0.840061	1.090499e-57	1.535512e-54
MsG0280010022.01	MsG0580029658.01	0.803567	3.278386e-49	1.653032e-46
MsG0280010022.01	MsG0580029801.01	0.835746	1.402280e-56	1.730720e-53
MsG0280010022.01	MsG0580030003.01	0.811938	5.450997e-51	3.419041e-48
MsG0280010022.01	MsG0680030436.01	0.802113	6.544523e-49	3.179329e-46
MsG0280010022.01	MsG0680030684.01	0.816165	6.365431e-52	4.476920e-49
MsG0280010022.01	MsG0680030740.01	0.825592	4.310763e-54	3.946397e-51
MsG0280010022.01	MsG0680031063.01	0.803184	3.934295e-49	1.964237e-46
MsG0280010022.01	MsG0680031133.01	0.825403	4.776710e-54	4.349608e-51
MsG0280010022.01	MsG0680031549.01	0.823176	1.595084e-53	1.363277e-50
MsG0280010022.01	MsG0680031839.01	0.820399	7.001508e-53	5.534254e-50
MsG0280010022.01	MsG0680034061.01	0.800489	1.407134e-48	6.560454e-46
MsG0280010022.01	MsG0680034757.01	0.810234	1.275498e-50	7.647258e-48
MsG0280010022.01	MsG0780036663.01	0.811748	5.993549e-51	3.740555e-48
MsG0280010022.01	MsG0780037975.01	0.813539	2.432085e-51	1.592664e-48
MsG0280010022.01	MsG0780038603.01	0.806521	7.902451e-50	4.298262e-47
MsG0280010022.01	MsG0780038937.01	0.802357	5.831169e-49	2.850630e-46
MsG0280010022.01	MsG0780039452.01	0.801057	1.077452e-48	5.096611e-46
MsG0280010022.01	MsG0780039712.01	0.807922	3.988200e-50	2.249509e-47
MsG0280010022.01	MsG0780039717.01	0.810895	9.179491e-51	5.601552e-48
MsG0280010022.01	MsG0780039845.01	0.846631	1.924364e-59	3.333017e-56
MsG0280010022.01	MsG0780039950.01	0.821867	3.212961e-53	2.645871e-50
MsG0280010022.01	MsG0780040047.01	0.808395	3.163632e-50	1.806886e-47
MsG0280010022.01	MsG0780040312.01	0.811851	5.694262e-51	3.563376e-48
MsG0280010022.01	MsG0780040488.01	0.801082	1.064835e-48	5.040040e-46
MsG0280010022.01	MsG0780040687.01	0.814344	1.616042e-51	1.081623e-48
MsG0280010022.01	MsG0780040688.01	0.807075	6.035351e-50	3.329866e-47
MsG0280010022.01	MsG0780040845.01	0.804775	1.836868e-49	9.554102e-47
MsG0280010022.01	MsG0780040880.01	0.800007	1.763694e-48	8.122781e-46
MsG0280010022.01	MsG0780041371.01	0.802137	6.469859e-49	3.145046e-46
MsG0280010022.01	MsG0780041423.01	0.857538	1.524806e-62	3.776145e-59
MsG0280010022.01	MsG0880042206.01	0.828595	8.238210e-55	8.225789e-52
MsG0280010022.01	MsG0880042547.01	0.834106	3.630703e-56	4.265910e-53
MsG0280010022.01	MsG0880042552.01	0.845506	3.891793e-59	6.499778e-56
MsG0280010022.01	MsG0880042760.01	0.852863	3.492011e-61	7.399795e-58
MsG0280010022.01	MsG0880042829.01	0.828599	8.220469e-55	8.209112e-52
MsG0280010022.01	MsG0880043252.01	0.813780	2.152404e-51	1.418665e-48
MsG0280010022.01	MsG0880043886.01	0.814942	1.191391e-51	8.103335e-49
MsG0280010022.01	MsG0880044075.01	0.851273	9.881753e-61	1.989465e-57
MsG0280010022.01	MsG0880044477.01	0.816057	6.727862e-52	4.718044e-49
MsG0280010022.01	MsG0880044546.01	0.802095	6.599489e-49	3.204662e-46
MsG0280010022.01	MsG0880044878.01	0.800518	1.388101e-48	6.476505e-46
MsG0280010022.01	MsG0880045165.01	0.810399	1.174944e-50	7.074876e-48
MsG0280010022.01	MsG0880045393.01	0.809056	2.285268e-50	1.328105e-47
MsG0280010022.01	MsG0880045433.01	-0.822341	2.494269e-53	2.082029e-50
MsG0280010022.01	MsG0880045583.01	0.803802	2.928996e-49	1.485732e-46
MsG0280010022.01	MsG0880045687.01	0.802656	5.059535e-49	2.492098e-46
MsG0280010022.01	MsG0880045838.01	0.813362	2.659576e-51	1.733289e-48
MsG0280010022.01	MsG0880045897.01	-0.808779	2.618573e-50	1.510570e-47
MsG0280010022.01	MsG0880045914.01	0.807148	5.823827e-50	3.219323e-47
MsG0280010022.01	MsG0880046027.01	0.834861	2.345584e-56	2.819074e-53
MsG0280010022.01	MsG0880046074.01	0.810372	1.191266e-50	7.168060e-48
MsG0280010022.01	MsG0880046684.01	0.835958	1.239161e-56	1.539078e-53
MsG0280010022.01	MsG0880046762.01	0.880574	4.907255e-70	2.781139e-66
MsG0280010022.01	MsG0880046933.01	0.819594	1.069762e-52	8.268227e-50
MsG0280010022.01	MsG0880047016.01	0.811738	6.025071e-51	3.759248e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010022.01.T01	MTR_2g076680	84.021	194	6	1	8	176	91	284	8.36e-101	305
MsG0280010022.01.T01	MTR_2g076680	90.278	72	7	0	162	233	424	495	5.83e-41	148

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010022.01.T01	AT1G16900	65.464	194	42	1	8	176	94	287	2.14e-76	242
MsG0280010022.01.T01	AT1G16900	77.778	72	16	0	162	233	426	497	4.98e-35	132

Find 50 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGCTGTGAGATTCTTTCT+TGG	0.247470	2:+62996176	MsG0280010022.01.T01:CDS
ACTATAACTATTATGGAAAA+TGG	0.250286	2:+62997075	MsG0280010022.01.T01:CDS
GTTACTGACTATAACTATTA+TGG	0.250541	2:+62997068	MsG0280010022.01.T01:CDS
GCAGTCGCTGTTTCTGCTAT+TGG	0.277671	2:+62996604	MsG0280010022.01.T01:CDS
CACCTAGGGTAGAATTAAAT+TGG	0.295021	2:-62999532	None:intergenic
TGAACTTGTCCCACAAAATC+TGG	0.297967	2:-62999473	None:intergenic
CAATGGATTGATGATGGTTT+TGG	0.307968	2:+62999494	MsG0280010022.01.T01:CDS
GTATGCAATCTCTCTTGCTT+CGG	0.332002	2:+62996552	MsG0280010022.01.T01:CDS
ATGTTCTTGTTGTTAAAGTA+TGG	0.342295	2:-62999572	None:intergenic
TATGCAATCTCTCTTGCTTC+GGG	0.358298	2:+62996553	MsG0280010022.01.T01:CDS
TCTGCTATTGGTGTGATACT+TGG	0.358995	2:+62996616	MsG0280010022.01.T01:CDS
CGACCAGCTTTGTGGGTGTT+TGG	0.360996	2:+62996033	MsG0280010022.01.T01:CDS
GTAGCTATTTCAAGAAAGTA+TGG	0.376006	2:+62996233	MsG0280010022.01.T01:CDS
CAGGTTCTGTACTTTGTGTT+GGG	0.400534	2:+62999414	MsG0280010022.01.T01:intron
TGGCTGGCCATTCTCAATCC+TGG	0.413538	2:+62996636	MsG0280010022.01.T01:CDS
CAAGTTCAATGGATTGATGA+TGG	0.415740	2:+62999488	MsG0280010022.01.T01:CDS
GCAGGTTCTGTACTTTGTGT+TGG	0.422829	2:+62999413	MsG0280010022.01.T01:intron
TACTTTGTGTTGGGAGTGAA+TGG	0.433620	2:+62999423	MsG0280010022.01.T01:CDS
GCAGTTCACAGTTTGCTCTT+CGG	0.447513	2:+62995974	MsG0280010022.01.T01:CDS
TAGTGGCTCGACCAGCTTTG+TGG	0.464263	2:+62996025	MsG0280010022.01.T01:CDS
TTCCAATTTAATTCTACCCT+AGG	0.487869	2:+62999530	MsG0280010022.01.T01:CDS
AGTGGCTCGACCAGCTTTGT+GGG	0.488898	2:+62996026	MsG0280010022.01.T01:CDS
GCATACATGGAAAACGAACT+TGG	0.489829	2:-62996535	None:intergenic
CTTCTAATATACAACGTAGC+TGG	0.490932	2:+62997116	MsG0280010022.01.T01:CDS
AGCAAGAGAGATTGCATACA+TGG	0.491027	2:-62996548	None:intergenic
GTGCGGCAGCAGTCCCACCT+AGG	0.496904	2:-62999547	None:intergenic
CTATTGGTGTGATACTTGGC+TGG	0.503293	2:+62996620	MsG0280010022.01.T01:CDS
AATTTAATTCTACCCTAGGT+GGG	0.510697	2:+62999534	MsG0280010022.01.T01:CDS
CAATTTAATTCTACCCTAGG+TGG	0.522327	2:+62999533	MsG0280010022.01.T01:CDS
GTGTCACTGGAAGAAAAGCC+AGG	0.529892	2:-62996654	None:intergenic
TAGGGTAGAATTAAATTGGA+AGG	0.530698	2:-62999528	None:intergenic
GAACTTGTCCCACAAAATCT+GGG	0.554074	2:-62999472	None:intergenic
TTCTTGTTGTTAAAGTATGG+TGG	0.556667	2:-62999569	None:intergenic
AGAAAAGCCAGGATTGAGAA+TGG	0.556831	2:-62996643	None:intergenic
TCACCAAACACCCACAAAGC+TGG	0.559084	2:-62996036	None:intergenic
TGCGGCAGCAGTCCCACCTA+GGG	0.576622	2:-62999546	None:intergenic
AGCTACCACAAGAACAGTCT+CGG	0.577523	2:-62996216	None:intergenic
ATATACAACGTAGCTGGAGG+CGG	0.584749	2:+62997122	MsG0280010022.01.T01:CDS
AGGGTAGAATTAAATTGGAA+GGG	0.596270	2:-62999527	None:intergenic
TGTCACCGAGACTGTTCTTG+TGG	0.598968	2:+62996211	MsG0280010022.01.T01:CDS
TTTACACACGTAGACTCGTA+AGG	0.608122	2:-63000666	None:intergenic
TCTGCTTGATAAACATGCTG+CGG	0.610372	2:+62996582	MsG0280010022.01.T01:CDS
CTAATATACAACGTAGCTGG+AGG	0.620873	2:+62997119	MsG0280010022.01.T01:CDS
AAGAACAGTCTCGGTGACAA+CGG	0.630148	2:-62996207	None:intergenic
AAACTCGTGTCTGACAACCT+TGG	0.635778	2:+63000686	MsG0280010022.01.T01:CDS
GCTAGCAAAGAAACAGCCGC+TGG	0.655782	2:-62996297	None:intergenic
GTTGTTAAAGTATGGTGGTG+CGG	0.662868	2:-62999564	None:intergenic
GCTATGCTGTGCTTAGCCAG+CGG	0.680101	2:+62996281	MsG0280010022.01.T01:CDS
ATACTTTAACAACAAGAACA+TGG	0.681934	2:+62999574	MsG0280010022.01.T01:CDS
AGAGAGAAGACTGAAAACCA+AGG	0.722310	2:-63000703	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTATTATATACACATTTAA+TGG	-	Chr2:62996980-62996999	None:intergenic	10.0%
!!	TTTATATATAAAATTCAAAC+AGG	+	Chr2:62996684-62996703	MsG0280010022.01.T01:intron	10.0%
!!	TATGAATAATTTACATTTCT+AGG	-	Chr2:62999682-62999701	None:intergenic	15.0%
!!!	AATTTTGTAAAAAAACTTGA+TGG	-	Chr2:62999247-62999266	None:intergenic	15.0%
!!!	ATAAAATAATAAAGAGTACA+TGG	-	Chr2:63000442-63000461	None:intergenic	15.0%
!!!	ATTTTGTAAAAAAACTTGAT+GGG	-	Chr2:62999246-62999265	None:intergenic	15.0%
!!!	TGTTTATTTTATTTTATCAG+TGG	-	Chr2:62999784-62999803	None:intergenic	15.0%
!!!	TTTTATTGAATAAGATTAAC+TGG	+	Chr2:62998857-62998876	MsG0280010022.01.T01:intron	15.0%
!!	ACTATAACTATTATGGAAAA+TGG	+	Chr2:62997075-62997094	MsG0280010022.01.T01:CDS	20.0%
!!	ATCTGTAGCAAAAATAAATA+AGG	-	Chr2:62996089-62996108	None:intergenic	20.0%
!!	ATTGAAAAAAAAAACCCTAA+AGG	+	Chr2:63000053-63000072	MsG0280010022.01.T01:intron	20.0%
!!	GATGATGATATCATTATTAT+TGG	+	Chr2:62997736-62997755	MsG0280010022.01.T01:intron	20.0%
!!	TATGCAAATTTAAAAAACAG+CGG	-	Chr2:62999004-62999023	None:intergenic	20.0%
!!	TATGTATATGTATCATTTAC+AGG	+	Chr2:63000619-63000638	MsG0280010022.01.T01:intron	20.0%
!!	TTGCAATAATGTAATTCTTT+AGG	-	Chr2:62997601-62997620	None:intergenic	20.0%
!!!	ATTTTGTATTTTTTTGTTGC+AGG	+	Chr2:62999395-62999414	MsG0280010022.01.T01:intron	20.0%
!!!	GGTTTTTTTTTTCAATGAAA+CGG	-	Chr2:63000049-63000068	None:intergenic	20.0%
!!!	TCAAGTTTTAATTTTTTTCC+TGG	+	Chr2:62999644-62999663	MsG0280010022.01.T01:intron	20.0%
!!!	TCTTTTATTTCATGAAATAG+TGG	+	Chr2:62996008-62996027	MsG0280010022.01.T01:CDS	20.0%
!!!	TGCATATATTTTATGTTACT+TGG	+	Chr2:63000588-63000607	MsG0280010022.01.T01:intron	20.0%
!!!	TTGGCAAATTTTTTTTTACA+TGG	+	Chr2:62997353-62997372	MsG0280010022.01.T01:intron	20.0%
!	AAAATCTGGTAAATCTCAAT+AGG	-	Chr2:62998762-62998781	None:intergenic	25.0%
!	AAAGAAAGAAAAAAAGCCAA+AGG	-	Chr2:62996336-62996355	None:intergenic	25.0%
!	AAATTCAAACAGGCATTTAT+TGG	+	Chr2:62996694-62996713	MsG0280010022.01.T01:intron	25.0%
!	ACAAAAAGAGTTAATTACCA+CGG	-	Chr2:62999029-62999048	None:intergenic	25.0%
!	ACTTATTTGATCTTATCTAC+TGG	+	Chr2:62998103-62998122	MsG0280010022.01.T01:intron	25.0%
!	ACTTGAATCTTGATTATTTG+AGG	+	Chr2:62999122-62999141	MsG0280010022.01.T01:intron	25.0%
!	AGAAACATATATGCAGTTAA+AGG	-	Chr2:62999623-62999642	None:intergenic	25.0%
!	ATACTTTAACAACAAGAACA+TGG	+	Chr2:62999574-62999593	MsG0280010022.01.T01:CDS	25.0%
!	ATATTAATCGCATAAACAAG+AGG	-	Chr2:62996120-62996139	None:intergenic	25.0%
!	GAAACTTAAGTTACATTAGA+AGG	-	Chr2:62999359-62999378	None:intergenic	25.0%
!	GGATCTTTATATATCTGATA+TGG	+	Chr2:62998599-62998618	MsG0280010022.01.T01:intron	25.0%
!	TAAGTTACATTAGAAGGTAA+TGG	-	Chr2:62999353-62999372	None:intergenic	25.0%
!	TCAAATCAAATTGTTTCACA+CGG	-	Chr2:62998227-62998246	None:intergenic	25.0%
!	TGATATCTCCTTTATACATT+TGG	+	Chr2:62997290-62997309	MsG0280010022.01.T01:intron	25.0%
!	TTTAATGTAATCACATGTGT+CGG	+	Chr2:62996867-62996886	MsG0280010022.01.T01:intron	25.0%
!!	AACACAGACATTTTTATTGA+AGG	-	Chr2:62996916-62996935	None:intergenic	25.0%
!!	ATGGTATAAGCATAATTTTC+AGG	-	Chr2:62996961-62996980	None:intergenic	25.0%
!!	CTGATGCAATAACATATTTT+TGG	+	Chr2:62998411-62998430	MsG0280010022.01.T01:intron	25.0%
!!	GTTACTGACTATAACTATTA+TGG	+	Chr2:62997068-62997087	MsG0280010022.01.T01:CDS	25.0%
!!	TAATTAACTCTTTTTGTGTC+TGG	+	Chr2:62999032-62999051	MsG0280010022.01.T01:intron	25.0%
!!	TATAAAGAATAAAGTGTCAC+TGG	-	Chr2:62996670-62996689	None:intergenic	25.0%
!!	TGTTTTTCTATTTCCTGTTT+TGG	+	Chr2:63000354-63000373	MsG0280010022.01.T01:intron	25.0%
!!	TTAAAAGTGTGAGAGTTATA+GGG	+	Chr2:62999726-62999745	MsG0280010022.01.T01:intron	25.0%
!!	TTTAGAGTTATCTTCTTCAT+TGG	+	Chr2:62997869-62997888	MsG0280010022.01.T01:intron	25.0%
!!!	ATGTTCTTGTTGTTAAAGTA+TGG	-	Chr2:62999575-62999594	None:intergenic	25.0%
!!!	ATTTTTATTGAAGGTGTGTT+CGG	-	Chr2:62996907-62996926	None:intergenic	25.0%
!!!	GCACTAAATTTTTAAAGTTG+CGG	+	Chr2:62997563-62997582	MsG0280010022.01.T01:intron	25.0%
!!!	TTTATTTTTGCTACAGATTG+AGG	+	Chr2:62996091-62996110	MsG0280010022.01.T01:intron	25.0%
	AAACCCAACCAAATGTATAA+AGG	-	Chr2:62997301-62997320	None:intergenic	30.0%
	AAATAGTATGTCACAAGAGA+GGG	-	Chr2:62996769-62996788	None:intergenic	30.0%
	AACATACCATAACACATTAG+AGG	-	Chr2:62999298-62999317	None:intergenic	30.0%
	AAGCTAAAGGAATAATCTTG+AGG	-	Chr2:62998326-62998345	None:intergenic	30.0%
	AAGGAGATATCACAATAAGT+AGG	-	Chr2:62997282-62997301	None:intergenic	30.0%
	AAGTGTGTATCAACTCATTT+AGG	-	Chr2:62999844-62999863	None:intergenic	30.0%
	AATGAAGCTATTATCCAACA+CGG	+	Chr2:62998300-62998319	MsG0280010022.01.T01:intron	30.0%
	AATGCCTGTAAAACATACAA+TGG	-	Chr2:62997041-62997060	None:intergenic	30.0%
	AATTGTTAAGTTAACCCACT+TGG	+	Chr2:62997899-62997918	MsG0280010022.01.T01:intron	30.0%
	AATTTAATTCTACCCTAGGT+GGG	+	Chr2:62999534-62999553	MsG0280010022.01.T01:CDS	30.0%
	ACACAAATAAGTGGATATAC+TGG	-	Chr2:62998470-62998489	None:intergenic	30.0%
	ACATTGCTGTAACAAAACAA+AGG	-	Chr2:63000569-63000588	None:intergenic	30.0%
	AGGGTAGAATTAAATTGGAA+GGG	-	Chr2:62999530-62999549	None:intergenic	30.0%
	ATATAATGTCTAAGAACACC+CGG	-	Chr2:62998686-62998705	None:intergenic	30.0%
	ATCTCCTTTATACATTTGGT+TGG	+	Chr2:62997294-62997313	MsG0280010022.01.T01:intron	30.0%
	ATGAAACACTTACACATACA+AGG	+	Chr2:62996789-62996808	MsG0280010022.01.T01:intron	30.0%
	ATTATTTCCATAAGCTCTTC+AGG	+	Chr2:62998197-62998216	MsG0280010022.01.T01:intron	30.0%
	ATTGTTAAGTTAACCCACTT+GGG	+	Chr2:62997900-62997919	MsG0280010022.01.T01:intron	30.0%
	ATTTGACTCTTTCTAAATGC+AGG	+	Chr2:62998642-62998661	MsG0280010022.01.T01:intron	30.0%
	CAAATGAATCATACTTGTCA+CGG	-	Chr2:62998531-62998550	None:intergenic	30.0%
	CACTTTACTTAAGATTCAGA+TGG	+	Chr2:62998578-62998597	MsG0280010022.01.T01:intron	30.0%
	CATTGCTGTAACAAAACAAA+GGG	-	Chr2:63000568-63000587	None:intergenic	30.0%
	CCATTCTTCAGATAAAAAAG+AGG	-	Chr2:62997176-62997195	None:intergenic	30.0%
	CTTGTATAAACCAAAAGTAG+TGG	-	Chr2:62997447-62997466	None:intergenic	30.0%
	GAAGTGAGAATTGTCATTAA+AGG	-	Chr2:62996378-62996397	None:intergenic	30.0%
	GAATCTTGAAATGCAGAATA+TGG	-	Chr2:62996435-62996454	None:intergenic	30.0%
	GCACTGAAAAAGGAAATAAA+TGG	+	Chr2:62998962-62998981	MsG0280010022.01.T01:intron	30.0%
	GCATATTTCTTCTTAGCAAT+AGG	-	Chr2:62997253-62997272	None:intergenic	30.0%
	TAAAACACTACATCAAATCC+AGG	-	Chr2:62997853-62997872	None:intergenic	30.0%
	TAAATAGTATGTCACAAGAG+AGG	-	Chr2:62996770-62996789	None:intergenic	30.0%
	TAGGGTAGAATTAAATTGGA+AGG	-	Chr2:62999531-62999550	None:intergenic	30.0%
	TAGTATTCCTTACAAATTGC+AGG	-	Chr2:62998929-62998948	None:intergenic	30.0%
	TCTCCTTTATACATTTGGTT+GGG	+	Chr2:62997295-62997314	MsG0280010022.01.T01:intron	30.0%
	TCTTGCAGATTGCAAATAAT+AGG	-	Chr2:62997477-62997496	None:intergenic	30.0%
	TGATTTCCTCTAATGTGTTA+TGG	+	Chr2:62999289-62999308	MsG0280010022.01.T01:intron	30.0%
	TGGAAGCAATAATTACTTTC+TGG	+	Chr2:62998619-62998638	MsG0280010022.01.T01:intron	30.0%
	TTAGAGAATGCATTATGACT+TGG	+	Chr2:62999804-62999823	MsG0280010022.01.T01:intron	30.0%
	TTCCAATTTAATTCTACCCT+AGG	+	Chr2:62999530-62999549	MsG0280010022.01.T01:CDS	30.0%
	TTGACACTTCTTAATAGTGA+AGG	+	Chr2:62998548-62998567	MsG0280010022.01.T01:intron	30.0%
	TTGGAACCTTATTAGTTGAT+GGG	+	Chr2:62997521-62997540	MsG0280010022.01.T01:intron	30.0%
!	AAACTTTAGTTCAGTATCAC+AGG	+	Chr2:62997667-62997686	MsG0280010022.01.T01:intron	30.0%
!	AACTTTAGTTCAGTATCACA+GGG	+	Chr2:62997668-62997687	MsG0280010022.01.T01:intron	30.0%
!	GTAACCATTGTATGTTTTAC+AGG	+	Chr2:62997034-62997053	MsG0280010022.01.T01:intron	30.0%
!	GTAGCTATTTCAAGAAAGTA+TGG	+	Chr2:62996233-62996252	MsG0280010022.01.T01:CDS	30.0%
!	GTTAAAAGTGTGAGAGTTAT+AGG	+	Chr2:62999725-62999744	MsG0280010022.01.T01:intron	30.0%
!	TAACTCTCACACTTTTAACA+TGG	-	Chr2:62999724-62999743	None:intergenic	30.0%
!	TGTAAAAAAACTTGATGGGT+CGG	-	Chr2:62999242-62999261	None:intergenic	30.0%
!	TTTTAAATTTGCATACTCCG+TGG	+	Chr2:62999009-62999028	MsG0280010022.01.T01:intron	30.0%
!!	TTCTTGTTGTTAAAGTATGG+TGG	-	Chr2:62999572-62999591	None:intergenic	30.0%
!!!	CCTCTTTTTTATCTGAAGAA+TGG	+	Chr2:62997173-62997192	MsG0280010022.01.T01:intron	30.0%
!!!	TATTTCCTGTTTTGGTTTTC+AGG	+	Chr2:63000362-63000381	MsG0280010022.01.T01:intron	30.0%
!!!	TTTTTTTCAATGAAACGGTG+AGG	-	Chr2:63000044-63000063	None:intergenic	30.0%
	ACTAAAGCCTGCAATTTGTA+AGG	+	Chr2:62998919-62998938	MsG0280010022.01.T01:intron	35.0%
	ACTTACACATACAAGGAAAC+TGG	+	Chr2:62996796-62996815	MsG0280010022.01.T01:intron	35.0%
	ATGTTGTCATCTCTCTCATT+AGG	+	Chr2:62999313-62999332	MsG0280010022.01.T01:intron	35.0%
	CAATTTAATTCTACCCTAGG+TGG	+	Chr2:62999533-62999552	MsG0280010022.01.T01:CDS	35.0%
	CACCTAGGGTAGAATTAAAT+TGG	-	Chr2:62999535-62999554	None:intergenic	35.0%
	CAGATAAAAAAGAGGTCCTT+CGG	-	Chr2:62997168-62997187	None:intergenic	35.0%
	CATCTTCATCAAGATCTAGA+AGG	-	Chr2:62997717-62997736	None:intergenic	35.0%
	CCAAACCTTTCTTCTTTATG+TGG	-	Chr2:62997337-62997356	None:intergenic	35.0%
	CCACATAAAGAAGAAAGGTT+TGG	+	Chr2:62997334-62997353	MsG0280010022.01.T01:intron	35.0%
	CCTACAACAGTAAAAGACAA+AGG	-	Chr2:62996145-62996164	None:intergenic	35.0%
	CTTCTAATATACAACGTAGC+TGG	+	Chr2:62997116-62997135	MsG0280010022.01.T01:CDS	35.0%
	CTTGGAACCTTATTAGTTGA+TGG	+	Chr2:62997520-62997539	MsG0280010022.01.T01:intron	35.0%
	GAACAAGGAAAAATGAGAGA+AGG	-	Chr2:63000135-63000154	None:intergenic	35.0%
	GATATCACAATAAGTAGGTC+TGG	-	Chr2:62997277-62997296	None:intergenic	35.0%
	TAAGTGGATATACTGGATAG+AGG	-	Chr2:62998463-62998482	None:intergenic	35.0%
	TATGCTGTGAGATTCTTTCT+TGG	+	Chr2:62996176-62996195	MsG0280010022.01.T01:CDS	35.0%
	TCACTCAAAGCATAAGCTAA+AGG	-	Chr2:62998339-62998358	None:intergenic	35.0%
	TGCAAGATCACGTAAAATAG+TGG	-	Chr2:62999209-62999228	None:intergenic	35.0%
	TTAAGTTATGCACACTTCAG+AGG	+	Chr2:62998037-62998056	MsG0280010022.01.T01:intron	35.0%
	TTCAAACAGGCATTTATTGG+TGG	+	Chr2:62996697-62996716	MsG0280010022.01.T01:intron	35.0%
	TTCGAAGAATGCACTGAAAA+AGG	+	Chr2:62998952-62998971	MsG0280010022.01.T01:intron	35.0%
!	AGAATGCATTATGACTTGGA+TGG	+	Chr2:62999808-62999827	MsG0280010022.01.T01:intron	35.0%
!	ATTATGACTTGGATGGGTTT+AGG	+	Chr2:62999815-62999834	MsG0280010022.01.T01:intron	35.0%
!	ATTTTGTGGGACAAGTTCAA+TGG	+	Chr2:62999477-62999496	MsG0280010022.01.T01:CDS	35.0%
!	ATTTTTCGTCCCAGATTTTG+TGG	+	Chr2:62999463-62999482	MsG0280010022.01.T01:CDS	35.0%
!	CCTTTGTCTTTTACTGTTGT+AGG	+	Chr2:62996142-62996161	MsG0280010022.01.T01:intron	35.0%
!	CGTACATTTTCTCTGATTCA+TGG	+	Chr2:62998727-62998746	MsG0280010022.01.T01:intron	35.0%
!	GAATGCATTATGACTTGGAT+GGG	+	Chr2:62999809-62999828	MsG0280010022.01.T01:intron	35.0%
!	TTTTCTATGCTTTGTCTGTC+TGG	+	Chr2:62997785-62997804	MsG0280010022.01.T01:intron	35.0%
!	TTTTTCGTCCCAGATTTTGT+GGG	+	Chr2:62999464-62999483	MsG0280010022.01.T01:CDS	35.0%
!!	CAAGTTCAATGGATTGATGA+TGG	+	Chr2:62999488-62999507	MsG0280010022.01.T01:CDS	35.0%
!!	TCTCATTTTTCCTTGTTCTG+TGG	+	Chr2:63000137-63000156	MsG0280010022.01.T01:intron	35.0%
!!	TCTCTTGTTTCCACTACTTT+TGG	+	Chr2:62997434-62997453	MsG0280010022.01.T01:intron	35.0%
!!	TTCTGACTTCTGAAACGAAA+AGG	-	Chr2:63000107-63000126	None:intergenic	35.0%
!!!	CAATGGATTGATGATGGTTT+TGG	+	Chr2:62999494-62999513	MsG0280010022.01.T01:CDS	35.0%
!!!	CTGGATTAGTTCTCTCTTTT+GGG	+	Chr2:62997804-62997823	MsG0280010022.01.T01:intron	35.0%
!!!	TCTGGATTAGTTCTCTCTTT+TGG	+	Chr2:62997803-62997822	MsG0280010022.01.T01:intron	35.0%
	AAAAGGAAGAGACGAGAGAA+AGG	-	Chr2:63000090-63000109	None:intergenic	40.0%
	AAGGTCGATCGTATACCTTT+AGG	-	Chr2:63000071-63000090	None:intergenic	40.0%
	AATTGAACCTGAGACTTGAG+GGG	-	Chr2:62997964-62997983	None:intergenic	40.0%
	ACCAACAAAACACACTGAGT+CGG	-	Chr2:63000317-63000336	None:intergenic	40.0%
	ACGTGTGTGTCAACTCATTT+AGG	-	Chr2:62999906-62999925	None:intergenic	40.0%
	ACTTGTCACGGAAAAGATCA+GGG	-	Chr2:62998519-62998538	None:intergenic	40.0%
	AGAAAAGCCAGGATTGAGAA+TGG	-	Chr2:62996646-62996665	None:intergenic	40.0%
	AGAATTGAACCTGAGACTTG+AGG	-	Chr2:62997966-62997985	None:intergenic	40.0%
	AGAGAGAAGACTGAAAACCA+AGG	-	Chr2:63000706-63000725	None:intergenic	40.0%
	AGAGGTAAACAGTAGCAACA+GGG	+	Chr2:62998055-62998074	MsG0280010022.01.T01:intron	40.0%
	AGCAAGAGAGATTGCATACA+TGG	-	Chr2:62996551-62996570	None:intergenic	40.0%
	AGCTAGCCCATCAACTAATA+AGG	-	Chr2:62997530-62997549	None:intergenic	40.0%
	AGGTCGATCGTATACCTTTA+GGG	-	Chr2:63000070-63000089	None:intergenic	40.0%
	AGGTTCCAAGTTCCTTAACA+AGG	-	Chr2:62997510-62997529	None:intergenic	40.0%
	AGTTGACACACACGTAGAAT+CGG	+	Chr2:62999911-62999930	MsG0280010022.01.T01:intron	40.0%
	ATCAGAGAAAATGTACGAGC+AGG	-	Chr2:62998724-62998743	None:intergenic	40.0%
	CAGGTTCTGTACTTTGTGTT+GGG	+	Chr2:62999414-62999433	MsG0280010022.01.T01:intron	40.0%
	CTAATATACAACGTAGCTGG+AGG	+	Chr2:62997119-62997138	MsG0280010022.01.T01:CDS	40.0%
	CTGGGACGAAAAATGATGAT+GGG	-	Chr2:62999457-62999476	None:intergenic	40.0%
	GAACTTGTCCCACAAAATCT+GGG	-	Chr2:62999475-62999494	None:intergenic	40.0%
	GAAGCAGCAACACAAATAAG+TGG	-	Chr2:62998479-62998498	None:intergenic	40.0%
	GAATTGAACCTGAGACTTGA+GGG	-	Chr2:62997965-62997984	None:intergenic	40.0%
	GCAACAATGTTGTAGTGTCA+TGG	-	Chr2:62999169-62999188	None:intergenic	40.0%
	GCATACATGGAAAACGAACT+TGG	-	Chr2:62996538-62996557	None:intergenic	40.0%
	GCTATGCTGTTCTTGGAATT+TGG	+	Chr2:62997227-62997246	MsG0280010022.01.T01:intron	40.0%
	GGTAAATCTCAATAGGAGCT+TGG	-	Chr2:62998755-62998774	None:intergenic	40.0%
	GGTTCCAAGTTCCTTAACAA+GGG	-	Chr2:62997509-62997528	None:intergenic	40.0%
	GTATGCAATCTCTCTTGCTT+CGG	+	Chr2:62996552-62996571	MsG0280010022.01.T01:CDS	40.0%
	GTTCCAAGTTCCTTAACAAG+GGG	-	Chr2:62997508-62997527	None:intergenic	40.0%
	TACTTGTCACGGAAAAGATC+AGG	-	Chr2:62998520-62998539	None:intergenic	40.0%
	TACTTTGTGTTGGGAGTGAA+TGG	+	Chr2:62999423-62999442	MsG0280010022.01.T01:CDS	40.0%
	TATGCAATCTCTCTTGCTTC+GGG	+	Chr2:62996553-62996572	MsG0280010022.01.T01:CDS	40.0%
	TCAGAGAAAATGTACGAGCA+GGG	-	Chr2:62998723-62998742	None:intergenic	40.0%
	TCGTAAGATATTGACAGCCA+TGG	-	Chr2:62999061-62999080	None:intergenic	40.0%
	TCTAATGCATCGAGCAGTTT+GGG	+	Chr2:62997625-62997644	MsG0280010022.01.T01:intron	40.0%
	TCTGCTATTGGTGTGATACT+TGG	+	Chr2:62996616-62996635	MsG0280010022.01.T01:CDS	40.0%
	TCTGCTTGATAAACATGCTG+CGG	+	Chr2:62996582-62996601	MsG0280010022.01.T01:CDS	40.0%
	TGAACTTGTCCCACAAAATC+TGG	-	Chr2:62999476-62999495	None:intergenic	40.0%
	TGCAGCCACATAAAGAAGAA+AGG	+	Chr2:62997329-62997348	MsG0280010022.01.T01:intron	40.0%
	TTAAGTTAACCCACTTGGGT+TGG	+	Chr2:62997904-62997923	MsG0280010022.01.T01:intron	40.0%
	TTCTAATGCATCGAGCAGTT+TGG	+	Chr2:62997624-62997643	MsG0280010022.01.T01:intron	40.0%
	TTTACACACGTAGACTCGTA+AGG	-	Chr2:63000669-63000688	None:intergenic	40.0%
	TTTCAGGCAAGAGTAAGCTT+AGG	-	Chr2:62996945-62996964	None:intergenic	40.0%
!	CATTTTTCCTTGTTCTGTGG+CGG	+	Chr2:63000140-63000159	MsG0280010022.01.T01:intron	40.0%
!	CTTTTTGTGTCTGGAAACCA+TGG	+	Chr2:62999041-62999060	MsG0280010022.01.T01:intron	40.0%
!	GAAGGTATGCTCTCAATGAT+TGG	-	Chr2:62997699-62997718	None:intergenic	40.0%
!	GCATTTTCAAATCACCACTG+TGG	-	Chr2:62998887-62998906	None:intergenic	40.0%
!	GTTGTTAAAGTATGGTGGTG+CGG	-	Chr2:62999567-62999586	None:intergenic	40.0%
!	TCTGGGACGAAAAATGATGA+TGG	-	Chr2:62999458-62999477	None:intergenic	40.0%
!	TGTTCTTGCTATGCTGTTCT+TGG	+	Chr2:62997220-62997239	MsG0280010022.01.T01:intron	40.0%
!!	ATCGATTGTTTGTTGTCTCC+AGG	+	Chr2:62996505-62996524	MsG0280010022.01.T01:intron	40.0%
!!	CAGTGTGTTTTGTTGGTTGT+TGG	+	Chr2:63000320-63000339	MsG0280010022.01.T01:intron	40.0%
!!!	TGGATTGATGATGGTTTTGG+AGG	+	Chr2:62999497-62999516	MsG0280010022.01.T01:CDS	40.0%
	AAACTCGTGTCTGACAACCT+TGG	+	Chr2:63000686-63000705	MsG0280010022.01.T01:CDS	45.0%
	AACGAACTTGGCAAGAAACC+TGG	-	Chr2:62996526-62996545	None:intergenic	45.0%
	AAGAACAGTCTCGGTGACAA+CGG	-	Chr2:62996210-62996229	None:intergenic	45.0%
	ACATTAATTCTCGCCGTCTC+TGG	+	Chr2:63000169-63000188	MsG0280010022.01.T01:intron	45.0%
	ACCACGACGATGTAGCAAAT+GGG	+	Chr2:62998787-62998806	MsG0280010022.01.T01:intron	45.0%
	AGAAAATGTACGAGCAGGGA+GGG	-	Chr2:62998719-62998738	None:intergenic	45.0%
	AGCACAAGTAAGTGAGCCTT+TGG	+	Chr2:62996317-62996336	MsG0280010022.01.T01:intron	45.0%
	AGCTACCACAAGAACAGTCT+CGG	-	Chr2:62996219-62996238	None:intergenic	45.0%
	AGTATGTCACAAGAGAGGGT+GGG	-	Chr2:62996765-62996784	None:intergenic	45.0%
	ATATACAACGTAGCTGGAGG+CGG	+	Chr2:62997122-62997141	MsG0280010022.01.T01:CDS	45.0%
	CAGAGCCTGAAAACCAAAAC+AGG	-	Chr2:63000370-63000389	None:intergenic	45.0%
	CAGAGGTAAACAGTAGCAAC+AGG	+	Chr2:62998054-62998073	MsG0280010022.01.T01:intron	45.0%
	CGGTTATCCTGAAGAGCTTA+TGG	-	Chr2:62998207-62998226	None:intergenic	45.0%
	CTATGCTAGTCACTGTAACC+TGG	+	Chr2:62997832-62997851	MsG0280010022.01.T01:intron	45.0%
	CTATTGGTGTGATACTTGGC+TGG	+	Chr2:62996620-62996639	MsG0280010022.01.T01:CDS	45.0%
	GAATAATCTTGAGGCCGTGT+TGG	-	Chr2:62998317-62998336	None:intergenic	45.0%
	GATTAACTGGACTACCACAG+TGG	+	Chr2:62998870-62998889	MsG0280010022.01.T01:intron	45.0%
	GATTGCTTCTCCCCTTGTTA+AGG	+	Chr2:62997495-62997514	MsG0280010022.01.T01:intron	45.0%
	GCAGGTTCTGTACTTTGTGT+TGG	+	Chr2:62999413-62999432	MsG0280010022.01.T01:intron	45.0%
	GCGGTGAAAGTCATCTGTAT+GGG	+	Chr2:62997141-62997160	MsG0280010022.01.T01:intron	45.0%
	GTCTCAGGTTCAATTCTCTC+CGG	+	Chr2:62997969-62997988	MsG0280010022.01.T01:intron	45.0%
	TAGTATGTCACAAGAGAGGG+TGG	-	Chr2:62996766-62996785	None:intergenic	45.0%
	TATACTGGATAGAGGAACCC+TGG	-	Chr2:62998455-62998474	None:intergenic	45.0%
	TCTCCCCTTGTTAAGGAACT+TGG	+	Chr2:62997502-62997521	MsG0280010022.01.T01:intron	45.0%
!	ATGACTTGGATGGGTTTAGG+AGG	+	Chr2:62999818-62999837	MsG0280010022.01.T01:intron	45.0%
!	GAGCAGTTTGGGATGGAATT+TGG	+	Chr2:62997636-62997655	MsG0280010022.01.T01:intron	45.0%
!	GCAGGTAGCCAAGTTTTCTA+AGG	+	Chr2:62998660-62998679	MsG0280010022.01.T01:intron	45.0%
!	GCAGTTCACAGTTTGCTCTT+CGG	+	Chr2:62995974-62995993	MsG0280010022.01.T01:CDS	45.0%
!	GGTGAGGCTGAATGTGTTTT+GGG	-	Chr2:63000028-63000047	None:intergenic	45.0%
!	TCCGACTCAGTGTGTTTTGT+TGG	+	Chr2:63000313-63000332	MsG0280010022.01.T01:intron	45.0%
!!!	GTAGCCAAGTTTTCTAAGGC+CGG	+	Chr2:62998664-62998683	MsG0280010022.01.T01:intron	45.0%
!!!	TAGCCAAGTTTTCTAAGGCC+GGG	+	Chr2:62998665-62998684	MsG0280010022.01.T01:intron	45.0%
	ACACCCGGCCTTAGAAAACT+TGG	-	Chr2:62998671-62998690	None:intergenic	50.0%
	ACGACGATGTAGCAAATGGG+AGG	+	Chr2:62998790-62998809	MsG0280010022.01.T01:intron	50.0%
	AGTCATCTGTATGGGACCGA+AGG	+	Chr2:62997149-62997168	MsG0280010022.01.T01:intron	50.0%
	ATGCATCGAGCAGTTTGGGA+TGG	+	Chr2:62997629-62997648	MsG0280010022.01.T01:intron	50.0%
	CACCACGACGATGTAGCAAA+TGG	+	Chr2:62998786-62998805	MsG0280010022.01.T01:intron	50.0%
	CCATCCTCTTTCCATGTCCA+GGG	+	Chr2:62998435-62998454	MsG0280010022.01.T01:intron	50.0%
	GAGAAAATGTACGAGCAGGG+AGG	-	Chr2:62998720-62998739	None:intergenic	50.0%
	GCCAACTTAGCCCACGAAAT+TGG	-	Chr2:62997997-62998016	None:intergenic	50.0%
	GGCGGTGAAAGTCATCTGTA+TGG	+	Chr2:62997140-62997159	MsG0280010022.01.T01:intron	50.0%
	GTAGTAGACTCGCTCTGACT+TGG	+	Chr2:62999866-62999885	MsG0280010022.01.T01:intron	50.0%
	TCACCAAACACCCACAAAGC+TGG	-	Chr2:62996039-62996058	None:intergenic	50.0%
	TCCATCCTCTTTCCATGTCC+AGG	+	Chr2:62998434-62998453	MsG0280010022.01.T01:intron	50.0%
	TCGGATGGAATCAGACAGAC+CGG	+	Chr2:62999957-62999976	MsG0280010022.01.T01:intron	50.0%
	TCTAGGAATCAACGACAGCC+AGG	-	Chr2:62999665-62999684	None:intergenic	50.0%
	TGTCACCGAGACTGTTCTTG+TGG	+	Chr2:62996211-62996230	MsG0280010022.01.T01:CDS	50.0%
	TTAGCCCACGAAATTGGCAC+CGG	-	Chr2:62997991-62998010	None:intergenic	50.0%
!	CGGTGAGGCTGAATGTGTTT+TGG	-	Chr2:63000029-63000048	None:intergenic	50.0%
!	GCCAATTTCGTGGGCTAAGT+TGG	+	Chr2:62997993-62998012	MsG0280010022.01.T01:intron	50.0%
!	GTGAATCGACTTAGACTCGC+CGG	-	Chr2:62999979-62999998	None:intergenic	50.0%
!	GTGTCACTGGAAGAAAAGCC+AGG	-	Chr2:62996657-62996676	None:intergenic	50.0%
!	TCCCATTTGCTACATCGTCG+TGG	-	Chr2:62998791-62998810	None:intergenic	50.0%
!!	CACATGTGTCGGCGTCATTT+TGG	+	Chr2:62996878-62996897	MsG0280010022.01.T01:intron	50.0%
!!	CGTCGTGGTGCTCTAAAATC+TGG	-	Chr2:62998776-62998795	None:intergenic	50.0%
!!	CTGACTTGGACGAGTTTAGG+AGG	+	Chr2:62999880-62999899	MsG0280010022.01.T01:intron	50.0%
!!	GCAGTCGCTGTTTCTGCTAT+TGG	+	Chr2:62996604-62996623	MsG0280010022.01.T01:CDS	50.0%
!!	GCTCTGACTTGGACGAGTTT+AGG	+	Chr2:62999877-62999896	MsG0280010022.01.T01:intron	50.0%
!!	GGGCTAGCTTACAGTGAATG+AGG	+	Chr2:62997541-62997560	MsG0280010022.01.T01:intron	50.0%
	AAATGTACGAGCAGGGAGGG+AGG	-	Chr2:62998716-62998735	None:intergenic	55.0%
	CCCTGGACATGGAAAGAGGA+TGG	-	Chr2:62998438-62998457	None:intergenic	55.0%
	GCTAGCAAAGAAACAGCCGC+TGG	-	Chr2:62996300-62996319	None:intergenic	55.0%
	GCTATGCTGTGCTTAGCCAG+CGG	+	Chr2:62996281-62996300	MsG0280010022.01.T01:CDS	55.0%
	GGAACCCTGGACATGGAAAG+AGG	-	Chr2:62998442-62998461	None:intergenic	55.0%
	GGATAGAGGAACCCTGGACA+TGG	-	Chr2:62998449-62998468	None:intergenic	55.0%
	GTCACAAGAGAGGGTGGGAA+AGG	-	Chr2:62996760-62996779	None:intergenic	55.0%
	TAGTGGCTCGACCAGCTTTG+TGG	+	Chr2:62996025-62996044	MsG0280010022.01.T01:CDS	55.0%
	TGAGACGTGCACACCAGAGA+CGG	-	Chr2:63000185-63000204	None:intergenic	55.0%
	TTGTGCTCCCCTCAAGTCTC+AGG	+	Chr2:62997954-62997973	MsG0280010022.01.T01:intron	55.0%
!	AGTGGCTCGACCAGCTTTGT+GGG	+	Chr2:62996026-62996045	MsG0280010022.01.T01:CDS	55.0%
!	CGACCAGCTTTGTGGGTGTT+TGG	+	Chr2:62996033-62996052	MsG0280010022.01.T01:CDS	55.0%
!	CTCTCCGGTGCCAATTTCGT+GGG	+	Chr2:62997984-62998003	MsG0280010022.01.T01:intron	55.0%
!	CTTGAGGGGAGCACAATCCA+AGG	-	Chr2:62997950-62997969	None:intergenic	55.0%
!	GCTCTGTTCTGCGAGTGTTG+CGG	+	Chr2:63000384-63000403	MsG0280010022.01.T01:intron	55.0%
!	TCTCTCCGGTGCCAATTTCG+TGG	+	Chr2:62997983-62998002	MsG0280010022.01.T01:intron	55.0%
!	TGGCTGGCCATTCTCAATCC+TGG	+	Chr2:62996636-62996655	MsG0280010022.01.T01:CDS	55.0%
!!	TTAACCCACTTGGGTTGGCC+TGG	+	Chr2:62997909-62997928	MsG0280010022.01.T01:intron	55.0%
	ACCACCAGGCCAACCCAAGT+GGG	-	Chr2:62997916-62997935	None:intergenic	60.0%
	AGGTCCAAGCCAACACCACC+AGG	-	Chr2:62997930-62997949	None:intergenic	60.0%
	TGTCGCACCGCCACAGAACA+AGG	-	Chr2:63000150-63000169	None:intergenic	60.0%
!!	GGTGGTGTTGGCTTGGACCT+TGG	+	Chr2:62997930-62997949	MsG0280010022.01.T01:intron	60.0%
!!	TTGGCCTGGTGGTGTTGGCT+TGG	+	Chr2:62997923-62997942	MsG0280010022.01.T01:intron	60.0%
!!	TTGGGTTGGCCTGGTGGTGT+TGG	+	Chr2:62997918-62997937	MsG0280010022.01.T01:intron	60.0%
	CACCACCAGGCCAACCCAAG+TGG	-	Chr2:62997917-62997936	None:intergenic	65.0%
	TGCGGCAGCAGTCCCACCTA+GGG	-	Chr2:62999549-62999568	None:intergenic	65.0%
!!	ACCCACTTGGGTTGGCCTGG+TGG	+	Chr2:62997912-62997931	MsG0280010022.01.T01:intron	65.0%
	GTGCGGCAGCAGTCCCACCT+AGG	-	Chr2:62999550-62999569	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	62995955	63000736	62995955	ID=MsG0280010022.01;Name=MsG0280010022.01
Chr2	mRNA	62995955	63000736	62995955	ID=MsG0280010022.01.T01;Parent=MsG0280010022.01;Name=MsG0280010022.01.T01;_AED=0.41;_eAED=0.49;_QI=0\|0.4\|0.33\|1\|1\|1\|6\|0\|264
Chr2	exon	62995955	62996065	62995955	ID=MsG0280010022.01.T01:exon:34877;Parent=MsG0280010022.01.T01
Chr2	exon	62996164	62996323	62996164	ID=MsG0280010022.01.T01:exon:34878;Parent=MsG0280010022.01.T01
Chr2	exon	62996527	62996669	62996527	ID=MsG0280010022.01.T01:exon:34879;Parent=MsG0280010022.01.T01
Chr2	exon	62997056	62997143	62997056	ID=MsG0280010022.01.T01:exon:34880;Parent=MsG0280010022.01.T01
Chr2	exon	62999417	62999613	62999417	ID=MsG0280010022.01.T01:exon:34881;Parent=MsG0280010022.01.T01
Chr2	exon	63000641	63000736	63000641	ID=MsG0280010022.01.T01:exon:34882;Parent=MsG0280010022.01.T01
Chr2	CDS	62995955	62996065	62995955	ID=MsG0280010022.01.T01:cds;Parent=MsG0280010022.01.T01
Chr2	CDS	62996164	62996323	62996164	ID=MsG0280010022.01.T01:cds;Parent=MsG0280010022.01.T01
Chr2	CDS	62996527	62996669	62996527	ID=MsG0280010022.01.T01:cds;Parent=MsG0280010022.01.T01
Chr2	CDS	62997056	62997143	62997056	ID=MsG0280010022.01.T01:cds;Parent=MsG0280010022.01.T01
Chr2	CDS	62999417	62999613	62999417	ID=MsG0280010022.01.T01:cds;Parent=MsG0280010022.01.T01
Chr2	CDS	63000641	63000736	63000641	ID=MsG0280010022.01.T01:cds;Parent=MsG0280010022.01.T01

Gene Sequence

>MsG0280010022.01.T01

ATGGAGTTGTGTGTGTGTTGCAGTTCACAGTTTGCTCTTCGGTCGTATTTGTATCTTTTATTTCATGAAATAGTGGCTCGACCAGCTTTGTGGGTGTTTGGTGATGATAAAGTGAGAGTGTTCTATGCTGTGAGATTCTTTCTTGGTTTTCTGTCCGTTGTCACCGAGACTGTTCTTGTGGTAGCTATTTCAAGAAAGTATGGAAAACGACTTGCTTGTTATACACTTGCTATGCTGTGCTTAGCCAGCGGCTGTTTCTTTGCTAGCACAAGTTTCTTGCCAAGTTCGTTTTCCATGTATGCAATCTCTCTTGCTTCGGGTTTATTTCTGCTTGATAAACATGCTGCGGCAGTCGCTGTTTCTGCTATTGGTGTGATACTTGGCTGGCCATTCTCAATCCTGGCTTTTCTTCCAGCATTGTCAGTTGTTACTGACTATAACTATTATGGAAAATGGACTTCATCAGTCTTGAATCTTCTAATATACAACGTAGCTGGAGGCGGTTCTGTACTTTGTGTTGGGAGTGAATGGCATCGTTACCCATCATCATTTTTCGTCCCAGATTTTGTGGGACAAGTTCAATGGATTGATGATGGTTTTGGAGGTCTTCTTCCCTTCCAATTTAATTCTACCCTAGGTGGGACTGCTGCCGCACCACCATACTTTAACAACAAGAACATGGCATCAGAAGAGCAATATGATTTTGCAAGTCACTTTTTATTATCCTTACGAGTCTACGTGTGTAAACTCGTGTCTGACAACCTTGGTTTTCAGTCTTCTCTCTGTCACATTTAA

Protein sequence

>MsG0280010022.01.T01

MELCVCCSSQFALRSYLYLLFHEIVARPALWVFGDDKVRVFYAVRFFLGFLSVVTETVLVVAISRKYGKRLACYTLAMLCLASGCFFASTSFLPSSFSMYAISLASGLFLLDKHAAAVAVSAIGVILGWPFSILAFLPALSVVTDYNYYGKWTSSVLNLLIYNVAGGGSVLCVGSEWHRYPSSFFVPDFVGQVQWIDDGFGGLLPFQFNSTLGGTAAAPPYFNNKNMASEEQYDFASHFLLSLRVYVCKLVSDNLGFQSSLCHI*