AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041142.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041142.01.T01 MTR_7g105030 94.186 86 5 0 8 93 585 670 3.24e-48 163
MsG0780041142.01.T01 MTR_7g105030 94.186 86 5 0 8 93 585 670 3.24e-48 163
MsG0780041142.01.T01 MTR_1g059730 63.529 85 31 0 8 92 589 673 8.92e-31 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041142.01.T01 AT2G36200 52.941 85 40 0 8 92 588 672 9.86e-23 91.3
MsG0780041142.01.T01 AT2G36200 52.941 85 40 0 8 92 619 703 9.87e-23 91.3

Find 19 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATGACCGTGGGAGGATA+TGG 0.333613 7:-87092710 None:intergenic
CTTCTGTGGCCGCAGTTGTA+AGG 0.441833 7:-87092848 None:intergenic
ACAACTGCGGCCACAGAAGC+TGG 0.453498 7:+87092852 MsG0780041142.01.T01:CDS
AAATGTTGTGCGTTTGCATA+AGG 0.469562 7:+87092651 MsG0780041142.01.T01:CDS
TCAAATCGATAGCCGCCTAC+AGG 0.494424 7:-87092574 None:intergenic
GGCAAGCTCAGATGCATCGT+TGG 0.542825 7:+87092672 MsG0780041142.01.T01:CDS
TTCAATTGAATGACCGTGGG+AGG 0.555275 7:-87092716 None:intergenic
GAGACACATACACCGCCCTC+AGG 0.567781 7:-87092612 None:intergenic
AAATATTGAGCCAGCTTCTG+TGG 0.569706 7:-87092862 None:intergenic
TTCTTCAATTGAATGACCGT+GGG 0.577395 7:-87092719 None:intergenic
AAGCTCAGATGCATCGTTGG+AGG 0.578206 7:+87092675 MsG0780041142.01.T01:CDS
CTTCTTCAATTGAATGACCG+TGG 0.584778 7:-87092720 None:intergenic
TCATCTGCCATATCCTCCCA+CGG 0.644594 7:+87092703 MsG0780041142.01.T01:CDS
AAAGAGAAATGAAAGACCTG+AGG 0.645617 7:+87092596 MsG0780041142.01.T01:CDS
GGTGTATGTGTCTCATATTG+AGG 0.648593 7:+87092621 MsG0780041142.01.T01:CDS
AGCTGTCTTTCAACACAACA+TGG 0.654858 7:+87092897 MsG0780041142.01.T01:CDS
AAGAGAAATGAAAGACCTGA+GGG 0.667452 7:+87092597 MsG0780041142.01.T01:CDS
GAATCAGTTCCTTACAACTG+CGG 0.678491 7:+87092839 MsG0780041142.01.T01:intron
AGAAATGAAAGACCTGAGGG+CGG 0.747256 7:+87092600 MsG0780041142.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATACAATAAAAAGGTGT+TGG - Chr7:87092761-87092780 None:intergenic 20.0%
!! TGAATTGACACTTTTTGCTA+GGG + Chr7:87092920-87092939 MsG0780041142.01.T01:CDS 30.0%
AAAGAGAAATGAAAGACCTG+AGG + Chr7:87092596-87092615 MsG0780041142.01.T01:CDS 35.0%
AAATGTTGTGCGTTTGCATA+AGG + Chr7:87092651-87092670 MsG0780041142.01.T01:CDS 35.0%
AAGAGAAATGAAAGACCTGA+GGG + Chr7:87092597-87092616 MsG0780041142.01.T01:CDS 35.0%
GCGTTGAAAACAATGAAAAG+TGG - Chr7:87092796-87092815 None:intergenic 35.0%
TTCTTCAATTGAATGACCGT+GGG - Chr7:87092722-87092741 None:intergenic 35.0%
!! GTGAATTGACACTTTTTGCT+AGG + Chr7:87092919-87092938 MsG0780041142.01.T01:CDS 35.0%
AAATATTGAGCCAGCTTCTG+TGG - Chr7:87092865-87092884 None:intergenic 40.0%
AGCTGTCTTTCAACACAACA+TGG + Chr7:87092897-87092916 MsG0780041142.01.T01:CDS 40.0%
CTTCTTCAATTGAATGACCG+TGG - Chr7:87092723-87092742 None:intergenic 40.0%
GAATCAGTTCCTTACAACTG+CGG + Chr7:87092839-87092858 MsG0780041142.01.T01:intron 40.0%
GGTGTATGTGTCTCATATTG+AGG + Chr7:87092621-87092640 MsG0780041142.01.T01:CDS 40.0%
AGAAATGAAAGACCTGAGGG+CGG + Chr7:87092600-87092619 MsG0780041142.01.T01:CDS 45.0%
TTCAATTGAATGACCGTGGG+AGG - Chr7:87092719-87092738 None:intergenic 45.0%
!! TAATTAATAATACAATAAAA+AGG - Chr7:87092767-87092786 None:intergenic 5.0%
AAGCTCAGATGCATCGTTGG+AGG + Chr7:87092675-87092694 MsG0780041142.01.T01:CDS 50.0%
TCAAATCGATAGCCGCCTAC+AGG - Chr7:87092577-87092596 None:intergenic 50.0%
TCATCTGCCATATCCTCCCA+CGG + Chr7:87092703-87092722 MsG0780041142.01.T01:CDS 50.0%
TGAATGACCGTGGGAGGATA+TGG - Chr7:87092713-87092732 None:intergenic 50.0%
CTTCTGTGGCCGCAGTTGTA+AGG - Chr7:87092851-87092870 None:intergenic 55.0%
GGCAAGCTCAGATGCATCGT+TGG + Chr7:87092672-87092691 MsG0780041142.01.T01:CDS 55.0%
ACAACTGCGGCCACAGAAGC+TGG + Chr7:87092852-87092871 MsG0780041142.01.T01:CDS 60.0%
GAGACACATACACCGCCCTC+AGG - Chr7:87092615-87092634 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 87092571 87092965 87092571 ID=MsG0780041142.01;Name=MsG0780041142.01
Chr7 mRNA 87092571 87092965 87092571 ID=MsG0780041142.01.T01;Parent=MsG0780041142.01;Name=MsG0780041142.01.T01;_AED=0.50;_eAED=0.53;_QI=0|1|0|1|1|1|2|0|96
Chr7 exon 87092571 87092741 87092571 ID=MsG0780041142.01.T01:exon:52149;Parent=MsG0780041142.01.T01
Chr7 exon 87092846 87092965 87092846 ID=MsG0780041142.01.T01:exon:52150;Parent=MsG0780041142.01.T01
Chr7 CDS 87092571 87092741 87092571 ID=MsG0780041142.01.T01:cds;Parent=MsG0780041142.01.T01
Chr7 CDS 87092846 87092965 87092846 ID=MsG0780041142.01.T01:cds;Parent=MsG0780041142.01.T01
Gene Sequence

>MsG0780041142.01.T01

TTGCCTGTAGGCGGCTATCGATTTGAAAGAGAAATGAAAGACCTGAGGGCGGTGTATGTGTCTCATATTGAGGCAGTGCAAAATGTTGTGCGTTTGCATAAGGCAAGCTCAGATGCATCGTTGGAGGAGTTATCATCTGCCATATCCTCCCACGGTCATTCAATTGAAGAATTCCTTACAACTGCGGCCACAGAAGCTGGCTCAATATTTGACGAGCTTCAGAGCTGTCTTTCAACACAACATGGTGAATTGACACTTTTTGCTAGGGAAGTACGCAATGTATGTGCTTGA

Protein sequence

>MsG0780041142.01.T01

LPVGGYRFEREMKDLRAVYVSHIEAVQNVVRLHKASSDASLEELSSAISSHGHSIEEFLTTAATEAGSIFDELQSCLSTQHGELTLFAREVRNVCA*