AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045460.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045460.01.T01 MTR_8g071970 62.500 64 24 0 1 64 301 364 1.07e-19 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGAAGCTTTAATGTTTA+AGG 0.137768 8:+59378026 None:intergenic
GTGAATGGTTTGATGAAGTT+TGG 0.274295 8:+59378102 MsG0880045460.01.T01:CDS
AACCCTAGTGTGGTAACTTA+TGG 0.294856 8:+59378072 MsG0880045460.01.T01:CDS
GTATAGCAGAAAAGGGAATA+TGG 0.360797 8:+59378214 MsG0880045460.01.T01:CDS
GAAGAAGCTTTAATGTTTAA+GGG 0.376771 8:+59378027 None:intergenic
GAAATTTGATGAGGCGAATT+CGG 0.383255 8:+59378125 MsG0880045460.01.T01:CDS
ATTGATGGGTATAGCAGAAA+AGG 0.409421 8:+59378206 MsG0880045460.01.T01:CDS
TGAATGGTTTGATGAAGTTT+GGG 0.411693 8:+59378103 MsG0880045460.01.T01:CDS
ACCCTAGTGTGGTAACTTAT+GGG 0.425186 8:+59378073 MsG0880045460.01.T01:CDS
TTGATGAGGCGAATTCGGTT+TGG 0.472053 8:+59378130 MsG0880045460.01.T01:CDS
ACTTATGGGATTCTTGTGAA+TGG 0.474154 8:+59378087 MsG0880045460.01.T01:CDS
GAATATGGATGATGCATTGA+GGG 0.497229 8:+59378229 MsG0880045460.01.T01:CDS
AAGCTTTAATGTTTAAGGGC+AGG 0.503168 8:+59378031 None:intergenic
TTGATGGGTATAGCAGAAAA+GGG 0.535214 8:+59378207 MsG0880045460.01.T01:CDS
ATCCCATAAGTTACCACACT+AGG 0.548985 8:-59378075 None:intergenic
TTTGGTTGAAATGTATAGCA+AGG 0.552220 8:+59378148 MsG0880045460.01.T01:CDS
TTGGTTGAAATGTATAGCAA+GGG 0.552257 8:+59378149 MsG0880045460.01.T01:CDS
GGAATATGGATGATGCATTG+AGG 0.579260 8:+59378228 MsG0880045460.01.T01:CDS
TTTAATGTTTAAGGGCAGGA+TGG 0.586680 8:+59378035 None:intergenic
TGGTTGAAATGTATAGCAAG+GGG 0.611244 8:+59378150 MsG0880045460.01.T01:CDS
TCCCATAAGTTACCACACTA+GGG 0.619965 8:-59378074 None:intergenic
GAAGTTTGGGAAATTTGATG+AGG 0.621584 8:+59378116 MsG0880045460.01.T01:CDS
GAATAAAGTGAACCCTAGTG+TGG 0.651615 8:+59378062 MsG0880045460.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAATGCACTTATTGAT+GGG + Chr8:59378192-59378211 MsG0880045460.01.T01:CDS 20.0%
! GCATTAAAAACAAACTCATT+AGG - Chr8:59378182-59378201 None:intergenic 25.0%
!! GTTTTTAATGCACTTATTGA+TGG + Chr8:59378191-59378210 MsG0880045460.01.T01:CDS 25.0%
TGAATGGTTTGATGAAGTTT+GGG + Chr8:59378103-59378122 MsG0880045460.01.T01:CDS 30.0%
TTGGTTGAAATGTATAGCAA+GGG + Chr8:59378149-59378168 MsG0880045460.01.T01:CDS 30.0%
TTTGGTTGAAATGTATAGCA+AGG + Chr8:59378148-59378167 MsG0880045460.01.T01:CDS 30.0%
ACTTATGGGATTCTTGTGAA+TGG + Chr8:59378087-59378106 MsG0880045460.01.T01:CDS 35.0%
GAAATTTGATGAGGCGAATT+CGG + Chr8:59378125-59378144 MsG0880045460.01.T01:CDS 35.0%
GAAGTTTGGGAAATTTGATG+AGG + Chr8:59378116-59378135 MsG0880045460.01.T01:CDS 35.0%
GTATAGCAGAAAAGGGAATA+TGG + Chr8:59378214-59378233 MsG0880045460.01.T01:CDS 35.0%
GTGAATGGTTTGATGAAGTT+TGG + Chr8:59378102-59378121 MsG0880045460.01.T01:CDS 35.0%
TGGTTGAAATGTATAGCAAG+GGG + Chr8:59378150-59378169 MsG0880045460.01.T01:CDS 35.0%
! ATTGATGGGTATAGCAGAAA+AGG + Chr8:59378206-59378225 MsG0880045460.01.T01:CDS 35.0%
! TTGATGGGTATAGCAGAAAA+GGG + Chr8:59378207-59378226 MsG0880045460.01.T01:CDS 35.0%
AACCCTAGTGTGGTAACTTA+TGG + Chr8:59378072-59378091 MsG0880045460.01.T01:CDS 40.0%
ACCCTAGTGTGGTAACTTAT+GGG + Chr8:59378073-59378092 MsG0880045460.01.T01:CDS 40.0%
ATCCCATAAGTTACCACACT+AGG - Chr8:59378078-59378097 None:intergenic 40.0%
GAATAAAGTGAACCCTAGTG+TGG + Chr8:59378062-59378081 MsG0880045460.01.T01:CDS 40.0%
TCCCATAAGTTACCACACTA+GGG - Chr8:59378077-59378096 None:intergenic 40.0%
TTGATGAGGCGAATTCGGTT+TGG + Chr8:59378130-59378149 MsG0880045460.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr8 gene 59378039 59378248 59378039 ID=MsG0880045460.01;Name=MsG0880045460.01
Chr8 mRNA 59378039 59378248 59378039 ID=MsG0880045460.01.T01;Parent=MsG0880045460.01;Name=MsG0880045460.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|0|1|0|69
Chr8 exon 59378039 59378248 59378039 ID=MsG0880045460.01.T01:exon:22878;Parent=MsG0880045460.01.T01
Chr8 CDS 59378039 59378248 59378039 ID=MsG0880045460.01.T01:cds;Parent=MsG0880045460.01.T01
Gene Sequence

>MsG0880045460.01.T01

ATGTTTAAGGGCAGGATGGTTGAGAATAAAGTGAACCCTAGTGTGGTAACTTATGGGATTCTTGTGAATGGTTTGATGAAGTTTGGGAAATTTGATGAGGCGAATTCGGTTTGGTTGAAATGTATAGCAAGGGGTTTTCTCCTAATGAGTTTGTTTTTAATGCACTTATTGATGGGTATAGCAGAAAAGGGAATATGGATGATGCATTGA

Protein sequence

>MsG0880045460.01.T01

MFKGRMVENKVNPSVVTYGILVNGLMKFGKFDEANSVWLKCIARGFLLMSLFLMHLLMGIAEKGIWMMH*