AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1366340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366340 MtrunA17_Chr1g0187671 97.512 201 0 2 1 200 1 197 3.85e-142 394
Msa1366340 MtrunA17_Chr1g0167411 72.487 189 43 2 1 180 1 189 3.71e-91 266
Msa1366340 MtrunA17_Chr4g0051251 81.119 143 22 1 44 186 39 176 2.57e-86 254
Msa1366340 MtrunA17_Chr5g0431211 78.571 140 30 0 50 189 31 170 3.19e-84 248
Msa1366340 MtrunA17_Chr7g0261381 93.333 135 9 0 52 186 60 194 8.26e-83 244
Msa1366340 MtrunA17_Chr1g0183761 94.656 131 7 0 56 186 45 175 1.43e-81 241
Msa1366340 MtrunA17_Chr2g0283861 71.622 148 42 0 43 190 22 169 1.38e-74 223
Msa1366340 MtrunA17_Chr3g0105921 80.597 134 26 0 56 189 42 175 2.50e-74 223
Msa1366340 MtrunA17_Chr2g0325831 68.212 151 42 1 29 179 2 146 9.71e-74 219
Msa1366340 MtrunA17_Chr4g0041141 68.707 147 41 1 33 179 11 152 1.92e-73 219
Msa1366340 MtrunA17_Chr2g0315791 75.182 137 34 0 54 190 31 167 7.77e-73 218
Msa1366340 MtrunA17_Chr7g0274631 65.839 161 48 3 28 187 4 158 9.92e-72 215
Msa1366340 MtrunA17_Chr1g0190231 83.200 125 21 0 56 180 40 164 8.70e-69 207
Msa1366340 MtrunA17_Chr8g0379061 73.016 126 34 0 54 179 83 208 2.38e-56 178
Msa1366340 MtrunA17_Chr1g0211641 80.435 46 9 0 49 94 64 109 3.67e-22 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366340 AT2G31160.1 81.595 163 20 1 35 187 23 185 4.44e-84 248
Msa1366340 AT1G07090.1 78.723 141 27 1 47 187 25 162 1.61e-83 246
Msa1366340 AT3G23290.2 78.235 170 29 1 19 180 3 172 7.58e-82 241
Msa1366340 AT5G58500.1 76.596 141 32 1 55 194 18 158 4.66e-80 236
Msa1366340 AT5G28490.1 81.379 145 23 1 41 185 14 154 1.03e-73 221
Msa1366340 AT3G04510.1 82.308 130 23 0 56 185 33 162 1.09e-70 214
Msa1366340 AT2G42610.1 71.233 146 39 1 42 187 14 156 1.91e-70 212
Msa1366340 AT2G42610.2 71.233 146 39 1 42 187 14 156 1.91e-70 212
Msa1366340 AT1G78815.1 70.079 127 38 0 53 179 37 163 5.65e-66 201
Msa1366340 AT4G18610.1 69.841 126 37 1 55 179 36 161 4.36e-63 194
Msa1366340 AT1G16910.1 65.909 132 45 0 53 184 20 151 2.12e-62 191

Find 33 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACAAGGTGCAGGTGGATT+TGG 0.285867 tig0018446:+216428 None:intergenic
CTTAGAGTGTTTGTCGTTGC+TGG 0.294103 tig0018446:+216629 None:intergenic
AGAAGAGACTGGAACACTTT+TGG 0.322091 tig0018446:-216587 Msa1366340:CDS
GACTCTTTCTTCGTGAAGTT+AGG 0.348358 tig0018446:-216289 Msa1366340:CDS
TTAAGGTATCTTGATCAGTT+TGG 0.366705 tig0018446:-216494 Msa1366340:CDS
TTGTCCTCTCAGACAAGCTT+GGG 0.367576 tig0018446:-216405 Msa1366340:CDS
GTGGTGGTCTATGGTTTCTT+AGG 0.368488 tig0018446:+216558 None:intergenic
AAACCTGAAGATAACCCTTT+TGG 0.390636 tig0018446:-216326 Msa1366340:CDS
GGTGGTGGAGGTTGAATTTG+AGG 0.396468 tig0018446:+216212 None:intergenic
ACTTAATTAGTTGCATTTGA+TGG 0.399489 tig0018446:+216188 None:intergenic
GCACCAAAAGGGTTATCTTC+AGG 0.403413 tig0018446:+216323 None:intergenic
ACTCTTTCTTCGTGAAGTTA+GGG 0.427767 tig0018446:-216288 Msa1366340:CDS
GTCTTCTGAGGTTGTTGTGT+TGG 0.429348 tig0018446:+216700 None:intergenic
CTTGTCCTCTCAGACAAGCT+TGG 0.437451 tig0018446:-216406 Msa1366340:CDS
GCATTTGATGGTGGTGGTGG+AGG 0.439596 tig0018446:+216200 None:intergenic
GTTGCATTTGATGGTGGTGG+TGG 0.444483 tig0018446:+216197 None:intergenic
GGAAGTCTTGATGCATTGAT+TGG 0.461550 tig0018446:-216383 Msa1366340:CDS
CTAACAGCACGAGCACCAAA+AGG 0.492363 tig0018446:+216311 None:intergenic
AGAGTGAAAGTGGTGGTCTA+TGG 0.493036 tig0018446:+216549 None:intergenic
TTAGTTGCATTTGATGGTGG+TGG 0.493298 tig0018446:+216194 None:intergenic
CTTTCACTCTCACGTTGTAG+TGG 0.531680 tig0018446:-216536 Msa1366340:CDS
AGAGGACAAGGACAAGGTGC+AGG 0.565916 tig0018446:+216419 None:intergenic
CAAGCTTGTCTGAGAGGACA+AGG 0.577230 tig0018446:+216407 None:intergenic
CTACAACGTGAGAGTGAAAG+TGG 0.580478 tig0018446:+216539 None:intergenic
CTTCCCCAAGCTTGTCTGAG+AGG 0.586547 tig0018446:+216401 None:intergenic
TAACAGCACGAGCACCAAAA+GGG 0.587993 tig0018446:+216312 None:intergenic
GGACAAGGACAAGGTGCAGG+TGG 0.600667 tig0018446:+216422 None:intergenic
GACTCTCAATCTAAAGCTAG+AGG 0.600860 tig0018446:-216266 Msa1366340:CDS
TAATTAGTTGCATTTGATGG+TGG 0.602526 tig0018446:+216191 None:intergenic
AGAGTGTTTGTCGTTGCTGG+TGG 0.637477 tig0018446:+216632 None:intergenic
TGTCCTCTCAGACAAGCTTG+GGG 0.658773 tig0018446:-216404 Msa1366340:CDS
TGTCTGAGAGGACAAGGACA+AGG 0.664143 tig0018446:+216413 None:intergenic
CAACGTGAGAGTGAAAGTGG+TGG 0.746060 tig0018446:+216542 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CACATGTTCTTGAATTTTTA+AGG - tig0018446:216449-216468 Msa1366340:CDS 25.0%
AGAATCAAAAAAGAAGAGAC+TGG - tig0018446:216362-216381 Msa1366340:CDS 30.0%
! TTAAGGTATCTTGATCAGTT+TGG - tig0018446:216466-216485 Msa1366340:CDS 30.0%
TGATCAGTTTGGAAAAACTA+AGG - tig0018446:216477-216496 Msa1366340:CDS 30.0%
!!! TCACAGATTTGTCCTTTTTT+TGG - tig0018446:216505-216524 Msa1366340:CDS 30.0%
TAATTAGTTGCATTTGATGG+TGG + tig0018446:216772-216791 None:intergenic 30.0%
!! GATGTTTTTGTTGTCTTCTG+AGG + tig0018446:216275-216294 None:intergenic 35.0%
GGATTTGGATGACCAAAAAA+AGG + tig0018446:216520-216539 None:intergenic 35.0%
! AAACCTGAAGATAACCCTTT+TGG - tig0018446:216634-216653 Msa1366340:CDS 35.0%
ACTCTTTCTTCGTGAAGTTA+GGG - tig0018446:216672-216691 Msa1366340:CDS 35.0%
! AGAAGAGACTGGAACACTTT+TGG - tig0018446:216373-216392 Msa1366340:CDS 40.0%
! GGAAGTCTTGATGCATTGAT+TGG - tig0018446:216577-216596 Msa1366340:CDS 40.0%
!!! AGAGCTGCTTTTGAAGAGAA+TGG - tig0018446:216607-216626 Msa1366340:CDS 40.0%
!! CTGCTTTTGAAGAGAATGGT+GGG - tig0018446:216611-216630 Msa1366340:CDS 40.0%
GACTCTTTCTTCGTGAAGTT+AGG - tig0018446:216671-216690 Msa1366340:CDS 40.0%
! GACTCTCAATCTAAAGCTAG+AGG - tig0018446:216694-216713 Msa1366340:CDS 40.0%
!! TTAGTTGCATTTGATGGTGG+TGG + tig0018446:216769-216788 None:intergenic 40.0%
!! GTCTTCTGAGGTTGTTGTGT+TGG + tig0018446:216263-216282 None:intergenic 45.0%
! CTTAGAGTGTTTGTCGTTGC+TGG + tig0018446:216334-216353 None:intergenic 45.0%
GTGGTGGTCTATGGTTTCTT+AGG + tig0018446:216405-216424 None:intergenic 45.0%
AGAGTGAAAGTGGTGGTCTA+TGG + tig0018446:216414-216433 None:intergenic 45.0%
CTACAACGTGAGAGTGAAAG+TGG + tig0018446:216424-216443 None:intergenic 45.0%
CTTTCACTCTCACGTTGTAG+TGG - tig0018446:216424-216443 Msa1366340:CDS 45.0%
TTGTCCTCTCAGACAAGCTT+GGG - tig0018446:216555-216574 Msa1366340:CDS 45.0%
!!! GCTGCTTTTGAAGAGAATGG+TGG - tig0018446:216610-216629 Msa1366340:CDS 45.0%
GCACCAAAAGGGTTATCTTC+AGG + tig0018446:216640-216659 None:intergenic 45.0%
TAACAGCACGAGCACCAAAA+GGG + tig0018446:216651-216670 None:intergenic 45.0%
! AGAGTGTTTGTCGTTGCTGG+TGG + tig0018446:216331-216350 None:intergenic 50.0%
CAACGTGAGAGTGAAAGTGG+TGG + tig0018446:216421-216440 None:intergenic 50.0%
TGTCTGAGAGGACAAGGACA+AGG + tig0018446:216550-216569 None:intergenic 50.0%
CAAGCTTGTCTGAGAGGACA+AGG + tig0018446:216556-216575 None:intergenic 50.0%
CTTGTCCTCTCAGACAAGCT+TGG - tig0018446:216554-216573 Msa1366340:CDS 50.0%
TGTCCTCTCAGACAAGCTTG+GGG - tig0018446:216556-216575 Msa1366340:CDS 50.0%
CTAACAGCACGAGCACCAAA+AGG + tig0018446:216652-216671 None:intergenic 50.0%
!! GGTGGTGGAGGTTGAATTTG+AGG + tig0018446:216751-216770 None:intergenic 50.0%
!! GTTGCATTTGATGGTGGTGG+TGG + tig0018446:216766-216785 None:intergenic 50.0%
! GGACAAGGTGCAGGTGGATT+TGG + tig0018446:216535-216554 None:intergenic 55.0%
AGAGGACAAGGACAAGGTGC+AGG + tig0018446:216544-216563 None:intergenic 55.0%
CTTCCCCAAGCTTGTCTGAG+AGG + tig0018446:216562-216581 None:intergenic 55.0%
!! GCATTTGATGGTGGTGGTGG+AGG + tig0018446:216763-216782 None:intergenic 55.0%
GGACAAGGACAAGGTGCAGG+TGG + tig0018446:216541-216560 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0018446 gene 216190 216792 216190 ID=Msa1366340;Name=Msa1366340
tig0018446 mRNA 216190 216792 216190 ID=Msa1366340-mRNA-1;Parent=Msa1366340;Name=Msa1366340-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|200
tig0018446 exon 216190 216792 216190 ID=Msa1366340-mRNA-1:exon:15105;Parent=Msa1366340-mRNA-1
tig0018446 CDS 216190 216792 216190 ID=Msa1366340-mRNA-1:cds;Parent=Msa1366340-mRNA-1
Gene Sequence

>Msa1366340

ATGAATTCATTTGAAGAATTCAACTCATCATCATCAACAAACACACAAACAAAAAACATCATCAACTTCACCAACACAACAACCTCAGAAGACAACAAAAACATCTCAAATTTCACATCTTCTTCTTCTTCAGCAGTACCACCAGCAACGACAAACACTCTAAGTCGCTATGAGAATCAAAAAAGAAGAGACTGGAACACTTTTGGTCAATACCTAAGAAACCATAGACCACCACTTTCACTCTCACGTTGTAGTGGAGCACATGTTCTTGAATTTTTAAGGTATCTTGATCAGTTTGGAAAAACTAAGGTTCACTCACAGATTTGTCCTTTTTTTGGTCATCCAAATCCACCTGCACCTTGTCCTTGTCCTCTCAGACAAGCTTGGGGAAGTCTTGATGCATTGATTGGAAGACTAAGAGCTGCTTTTGAAGAGAATGGTGGGAAACCTGAAGATAACCCTTTTGGTGCTCGTGCTGTTAGACTCTTTCTTCGTGAAGTTAGGGACTCTCAATCTAAAGCTAGAGGTATTAGCTATGAGAAAAAGAAGCGTAAAAGACCTCAAATTCAACCTCCACCACCACCATCAAATGCAACTAATTAA

Protein sequence

>Msa1366340

MNSFEEFNSSSSTNTQTKNIINFTNTTTSEDNKNISNFTSSSSSAVPPATTNTLSRYENQKRRDWNTFGQYLRNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHSQICPFFGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPEDNPFGARAVRLFLREVRDSQSKARGISYEKKKRKRPQIQPPPPPSNATN*