AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0242270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0242270 MtrunA17_Chr2g0294391 95.714 70 3 0 3 72 352 421 3.50e-27 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTGAGCATCTCTTAT+TGG 0.255116 2_2:-18371547 Msa0242270:CDS
AGCATCTCTTATTGGCTCTA+AGG 0.283609 2_2:-18371539 Msa0242270:CDS
TCTAAGGGAAGCACTTCCTT+TGG 0.376938 2_2:-18371523 Msa0242270:CDS
TTAATTAGCGGCATAGCTTA+TGG 0.378193 2_2:-18371816 Msa0242270:CDS
TGCATGCATGTTCAGATTGT+TGG 0.410316 2_2:-18371624 Msa0242270:intron
TGAAGCAGCTCCACCATGTT+GGG 0.410865 2_2:+18371578 None:intergenic
TTGTTTGTTTCCAGAATACT+TGG 0.412856 2_2:-18371883 Msa0242270:intron
AGGGAAGCACTTCCTTTGGT+TGG 0.480501 2_2:-18371519 Msa0242270:CDS
AGAGCCAATAAGAGATGCTC+AGG 0.491628 2_2:+18371543 None:intergenic
CCATCATGTGCGTTAATTAG+CGG 0.492981 2_2:-18371828 Msa0242270:CDS
CCGCTAATTAACGCACATGA+TGG 0.532055 2_2:+18371828 None:intergenic
GCATCTCTTATTGGCTCTAA+GGG 0.555107 2_2:-18371538 Msa0242270:CDS
TTGAAGCAGCTCCACCATGT+TGG 0.555464 2_2:+18371577 None:intergenic
TCCACCATGTTGGGATTGCA+TGG 0.562511 2_2:+18371587 None:intergenic
TTATGGTGGCTACGCGCGAA+AGG 0.564513 2_2:-18371799 Msa0242270:intron
TAGAAACATCAACTTATGGC+AGG 0.574581 2_2:-18372624 None:intergenic
CATTTAACAGGTCCAACCAA+AGG 0.645583 2_2:+18371507 None:intergenic
AGATCCATGCAATCCCAACA+TGG 0.647702 2_2:-18371591 Msa0242270:CDS
ATTAGCGGCATAGCTTATGG+TGG 0.665988 2_2:-18371813 Msa0242270:CDS
TCCATGCAATCCCAACATGG+TGG 0.701127 2_2:-18371588 Msa0242270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAACATGGAATAGAATT+TGG + chr2_2:18371577-18371596 None:intergenic 20.0%
!! ATGTATAAACTAAAGTTAGA+CGG - chr2_2:18371617-18371636 Msa0242270:intron 20.0%
!! AATAAACATGGAATAGAATT+TGG + chr2_2:18371577-18371596 None:intergenic 20.0%
!! ATGTATAAACTAAAGTTAGA+CGG - chr2_2:18371617-18371636 Msa0242270:intron 20.0%
!!! TGGGTTTGTTAAAATAAACA+TGG + chr2_2:18371589-18371608 None:intergenic 25.0%
!! GTCTAACTTTAGTTTATACA+TGG + chr2_2:18371618-18371637 None:intergenic 25.0%
!!! GGAAAAGATTTTAATGAGAT+AGG - chr2_2:18371857-18371876 Msa0242270:CDS 25.0%
! GACATGATATGAATCTAAAA+TGG - chr2_2:18371944-18371963 Msa0242270:intron 25.0%
! ATTGAAGGATTGAAAAAAGA+AGG - chr2_2:18372110-18372129 Msa0242270:intron 25.0%
! TCAGACATACATAAATAGTT+GGG + chr2_2:18372354-18372373 None:intergenic 25.0%
! ATCAGACATACATAAATAGT+TGG + chr2_2:18372355-18372374 None:intergenic 25.0%
! TTATCTGACTACAGAAAATT+TGG + chr2_2:18372404-18372423 None:intergenic 25.0%
!!! TGGGTTTGTTAAAATAAACA+TGG + chr2_2:18371589-18371608 None:intergenic 25.0%
!! GTCTAACTTTAGTTTATACA+TGG + chr2_2:18371618-18371637 None:intergenic 25.0%
!!! GGAAAAGATTTTAATGAGAT+AGG - chr2_2:18371857-18371876 Msa0242270:CDS 25.0%
! GACATGATATGAATCTAAAA+TGG - chr2_2:18371944-18371963 Msa0242270:intron 25.0%
! ATTGAAGGATTGAAAAAAGA+AGG - chr2_2:18372110-18372129 Msa0242270:intron 25.0%
! TCAGACATACATAAATAGTT+GGG + chr2_2:18372354-18372373 None:intergenic 25.0%
! ATCAGACATACATAAATAGT+TGG + chr2_2:18372355-18372374 None:intergenic 25.0%
! TTATCTGACTACAGAAAATT+TGG + chr2_2:18372404-18372423 None:intergenic 25.0%
AGTTTATACATGGCAAAGAT+GGG + chr2_2:18371608-18371627 None:intergenic 30.0%
TAGTTTATACATGGCAAAGA+TGG + chr2_2:18371609-18371628 None:intergenic 30.0%
!! AATAGCCTTTGTTGTTCAAT+TGG - chr2_2:18371685-18371704 Msa0242270:intron 30.0%
TTTAGCCAATTGAACAACAA+AGG + chr2_2:18371693-18371712 None:intergenic 30.0%
GATAGGATATGGATATGATA+AGG - chr2_2:18371806-18371825 Msa0242270:CDS 30.0%
!! AAGATTTTAATGAGATAGGC+AGG - chr2_2:18371861-18371880 Msa0242270:CDS 30.0%
GTCAAGTTATCATATCATGA+TGG - chr2_2:18371901-18371920 Msa0242270:intron 30.0%
ATGATATGTATAAACCCTGA+AGG + chr2_2:18372017-18372036 None:intergenic 30.0%
AATGTTTCATTCCCTTAACA+TGG - chr2_2:18372046-18372065 Msa0242270:intron 30.0%
TTGTTTGTTTCCAGAATACT+TGG - chr2_2:18372241-18372260 Msa0242270:intron 30.0%
! TTTTCATGATCCAAGTATTC+TGG + chr2_2:18372254-18372273 None:intergenic 30.0%
AGTTTATACATGGCAAAGAT+GGG + chr2_2:18371608-18371627 None:intergenic 30.0%
TAGTTTATACATGGCAAAGA+TGG + chr2_2:18371609-18371628 None:intergenic 30.0%
!! AATAGCCTTTGTTGTTCAAT+TGG - chr2_2:18371685-18371704 Msa0242270:intron 30.0%
TTTAGCCAATTGAACAACAA+AGG + chr2_2:18371693-18371712 None:intergenic 30.0%
GATAGGATATGGATATGATA+AGG - chr2_2:18371806-18371825 Msa0242270:CDS 30.0%
!! AAGATTTTAATGAGATAGGC+AGG - chr2_2:18371861-18371880 Msa0242270:CDS 30.0%
GTCAAGTTATCATATCATGA+TGG - chr2_2:18371901-18371920 Msa0242270:intron 30.0%
ATGATATGTATAAACCCTGA+AGG + chr2_2:18372017-18372036 None:intergenic 30.0%
AATGTTTCATTCCCTTAACA+TGG - chr2_2:18372046-18372065 Msa0242270:intron 30.0%
TTGTTTGTTTCCAGAATACT+TGG - chr2_2:18372241-18372260 Msa0242270:intron 30.0%
! TTTTCATGATCCAAGTATTC+TGG + chr2_2:18372254-18372273 None:intergenic 30.0%
TTGAATTGTGAATACGAACC+AGG + chr2_2:18371731-18371750 None:intergenic 35.0%
TCTCTATCATGACCATCAAA+CGG + chr2_2:18371784-18371803 None:intergenic 35.0%
! TTTGATGGTCATGATAGAGA+CGG - chr2_2:18371784-18371803 Msa0242270:intron 35.0%
! ATAAGGTCTTATCCTCATGT+TGG - chr2_2:18371823-18371842 Msa0242270:CDS 35.0%
ACTAATGCTCACACAATTGA+AGG - chr2_2:18372095-18372114 Msa0242270:intron 35.0%
TTAATTAGCGGCATAGCTTA+TGG - chr2_2:18372308-18372327 Msa0242270:intron 35.0%
CTCACATTATGTGATGACAT+TGG - chr2_2:18372450-18372469 Msa0242270:intron 35.0%
TTGAATTGTGAATACGAACC+AGG + chr2_2:18371731-18371750 None:intergenic 35.0%
TCTCTATCATGACCATCAAA+CGG + chr2_2:18371784-18371803 None:intergenic 35.0%
! TTTGATGGTCATGATAGAGA+CGG - chr2_2:18371784-18371803 Msa0242270:intron 35.0%
! ATAAGGTCTTATCCTCATGT+TGG - chr2_2:18371823-18371842 Msa0242270:CDS 35.0%
ACTAATGCTCACACAATTGA+AGG - chr2_2:18372095-18372114 Msa0242270:intron 35.0%
TTAATTAGCGGCATAGCTTA+TGG - chr2_2:18372308-18372327 Msa0242270:intron 35.0%
CTCACATTATGTGATGACAT+TGG - chr2_2:18372450-18372469 Msa0242270:intron 35.0%
AATTGTGAATACGAACCAGG+CGG + chr2_2:18371728-18371747 None:intergenic 40.0%
! AGTCCTTTGAAAACCTTCAC+AGG - chr2_2:18371756-18371775 Msa0242270:intron 40.0%
CTCTATCATGACCATCAAAC+GGG + chr2_2:18371783-18371802 None:intergenic 40.0%
TGATAGAGACGGATAGGATA+TGG - chr2_2:18371795-18371814 Msa0242270:intron 40.0%
!! CCTCATGTTGGTTTCTTACT+CGG - chr2_2:18371835-18371854 Msa0242270:CDS 40.0%
!! CTCATGTTGGTTTCTTACTC+GGG - chr2_2:18371836-18371855 Msa0242270:CDS 40.0%
CAGGATAAGAGAGACATGAT+AGG + chr2_2:18371981-18372000 None:intergenic 40.0%
TTTCATTCCCTTAACATGGC+AGG - chr2_2:18372050-18372069 Msa0242270:intron 40.0%
AAGAAAGCCTGCCATGTTAA+GGG + chr2_2:18372060-18372079 None:intergenic 40.0%
GTTTAAGTAATGCTGTCACG+TGG - chr2_2:18372150-18372169 Msa0242270:intron 40.0%
CCATCATGTGCGTTAATTAG+CGG - chr2_2:18372296-18372315 Msa0242270:intron 40.0%
TGCATGCATGTTCAGATTGT+TGG - chr2_2:18372500-18372519 Msa0242270:intron 40.0%
TCATCCTGAGCATCTCTTAT+TGG - chr2_2:18372577-18372596 Msa0242270:intron 40.0%
!! AGCATCTCTTATTGGCTCTA+AGG - chr2_2:18372585-18372604 Msa0242270:intron 40.0%
!! GCATCTCTTATTGGCTCTAA+GGG - chr2_2:18372586-18372605 Msa0242270:intron 40.0%
AATTGTGAATACGAACCAGG+CGG + chr2_2:18371728-18371747 None:intergenic 40.0%
! AGTCCTTTGAAAACCTTCAC+AGG - chr2_2:18371756-18371775 Msa0242270:intron 40.0%
CTCTATCATGACCATCAAAC+GGG + chr2_2:18371783-18371802 None:intergenic 40.0%
TGATAGAGACGGATAGGATA+TGG - chr2_2:18371795-18371814 Msa0242270:intron 40.0%
!! CCTCATGTTGGTTTCTTACT+CGG - chr2_2:18371835-18371854 Msa0242270:CDS 40.0%
!! CTCATGTTGGTTTCTTACTC+GGG - chr2_2:18371836-18371855 Msa0242270:CDS 40.0%
CAGGATAAGAGAGACATGAT+AGG + chr2_2:18371981-18372000 None:intergenic 40.0%
TTTCATTCCCTTAACATGGC+AGG - chr2_2:18372050-18372069 Msa0242270:intron 40.0%
AAGAAAGCCTGCCATGTTAA+GGG + chr2_2:18372060-18372079 None:intergenic 40.0%
GTTTAAGTAATGCTGTCACG+TGG - chr2_2:18372150-18372169 Msa0242270:intron 40.0%
CCATCATGTGCGTTAATTAG+CGG - chr2_2:18372296-18372315 Msa0242270:intron 40.0%
TGCATGCATGTTCAGATTGT+TGG - chr2_2:18372500-18372519 Msa0242270:intron 40.0%
TCATCCTGAGCATCTCTTAT+TGG - chr2_2:18372577-18372596 Msa0242270:intron 40.0%
!! AGCATCTCTTATTGGCTCTA+AGG - chr2_2:18372585-18372604 Msa0242270:intron 40.0%
!! GCATCTCTTATTGGCTCTAA+GGG - chr2_2:18372586-18372605 Msa0242270:intron 40.0%
TGTCCGATGAGTGGAGATAT+TGG - chr2_2:18371641-18371660 Msa0242270:intron 45.0%
GTCCGATGAGTGGAGATATT+GGG - chr2_2:18371642-18371661 Msa0242270:intron 45.0%
CTCCCAATATCTCCACTCAT+CGG + chr2_2:18371647-18371666 None:intergenic 45.0%
ATGAGTGGAGATATTGGGAG+AGG - chr2_2:18371647-18371666 Msa0242270:intron 45.0%
TATTGGGAGAGGAGCAACTA+AGG - chr2_2:18371658-18371677 Msa0242270:intron 45.0%
AAAGCAAAAGATAGACCGCC+TGG - chr2_2:18371710-18371729 Msa0242270:intron 45.0%
! GGTCCTGTGAAGGTTTTCAA+AGG + chr2_2:18371762-18371781 None:intergenic 45.0%
TGGTCATGATAGAGACGGAT+AGG - chr2_2:18371789-18371808 Msa0242270:intron 45.0%
CCGAGTAAGAAACCAACATG+AGG + chr2_2:18371838-18371857 None:intergenic 45.0%
GCAGGATATGATATGCGACA+TGG - chr2_2:18371879-18371898 Msa0242270:intron 45.0%
TCTTATCCTGACCATCGAAC+AGG - chr2_2:18371991-18372010 Msa0242270:intron 45.0%
GAAGAAAGCCTGCCATGTTA+AGG + chr2_2:18372061-18372080 None:intergenic 45.0%
CCGCTAATTAACGCACATGA+TGG + chr2_2:18372299-18372318 None:intergenic 45.0%
ATTAGCGGCATAGCTTATGG+TGG - chr2_2:18372311-18372330 Msa0242270:intron 45.0%
AGATCCATGCAATCCCAACA+TGG - chr2_2:18372533-18372552 Msa0242270:intron 45.0%
AGAGCCAATAAGAGATGCTC+AGG + chr2_2:18372584-18372603 None:intergenic 45.0%
!! TCTAAGGGAAGCACTTCCTT+TGG - chr2_2:18372601-18372620 Msa0242270:intron 45.0%
TGTCCGATGAGTGGAGATAT+TGG - chr2_2:18371641-18371660 Msa0242270:intron 45.0%
GTCCGATGAGTGGAGATATT+GGG - chr2_2:18371642-18371661 Msa0242270:intron 45.0%
CTCCCAATATCTCCACTCAT+CGG + chr2_2:18371647-18371666 None:intergenic 45.0%
ATGAGTGGAGATATTGGGAG+AGG - chr2_2:18371647-18371666 Msa0242270:intron 45.0%
TATTGGGAGAGGAGCAACTA+AGG - chr2_2:18371658-18371677 Msa0242270:intron 45.0%
AAAGCAAAAGATAGACCGCC+TGG - chr2_2:18371710-18371729 Msa0242270:intron 45.0%
! GGTCCTGTGAAGGTTTTCAA+AGG + chr2_2:18371762-18371781 None:intergenic 45.0%
TGGTCATGATAGAGACGGAT+AGG - chr2_2:18371789-18371808 Msa0242270:intron 45.0%
CCGAGTAAGAAACCAACATG+AGG + chr2_2:18371838-18371857 None:intergenic 45.0%
GCAGGATATGATATGCGACA+TGG - chr2_2:18371879-18371898 Msa0242270:intron 45.0%
TCTTATCCTGACCATCGAAC+AGG - chr2_2:18371991-18372010 Msa0242270:intron 45.0%
GAAGAAAGCCTGCCATGTTA+AGG + chr2_2:18372061-18372080 None:intergenic 45.0%
CCGCTAATTAACGCACATGA+TGG + chr2_2:18372299-18372318 None:intergenic 45.0%
ATTAGCGGCATAGCTTATGG+TGG - chr2_2:18372311-18372330 Msa0242270:intron 45.0%
AGATCCATGCAATCCCAACA+TGG - chr2_2:18372533-18372552 Msa0242270:intron 45.0%
AGAGCCAATAAGAGATGCTC+AGG + chr2_2:18372584-18372603 None:intergenic 45.0%
!! TCTAAGGGAAGCACTTCCTT+TGG - chr2_2:18372601-18372620 Msa0242270:intron 45.0%
GCAGGCTTTCTTCAACTAGC+TGG - chr2_2:18372068-18372087 Msa0242270:intron 50.0%
GGCTTTCTTCAACTAGCTGG+TGG - chr2_2:18372071-18372090 Msa0242270:intron 50.0%
TCCATGCAATCCCAACATGG+TGG - chr2_2:18372536-18372555 Msa0242270:intron 50.0%
TCCACCATGTTGGGATTGCA+TGG + chr2_2:18372540-18372559 None:intergenic 50.0%
TGAAGCAGCTCCACCATGTT+GGG + chr2_2:18372549-18372568 None:intergenic 50.0%
TTGAAGCAGCTCCACCATGT+TGG + chr2_2:18372550-18372569 None:intergenic 50.0%
!! AGGGAAGCACTTCCTTTGGT+TGG - chr2_2:18372605-18372624 Msa0242270:intron 50.0%
AGAAATGCCACACCTGCCAT+AGG + chr2_2:18371515-18371534 None:intergenic 50.0%
GCAGGCTTTCTTCAACTAGC+TGG - chr2_2:18372068-18372087 Msa0242270:intron 50.0%
GGCTTTCTTCAACTAGCTGG+TGG - chr2_2:18372071-18372090 Msa0242270:intron 50.0%
TCCATGCAATCCCAACATGG+TGG - chr2_2:18372536-18372555 Msa0242270:intron 50.0%
TCCACCATGTTGGGATTGCA+TGG + chr2_2:18372540-18372559 None:intergenic 50.0%
TGAAGCAGCTCCACCATGTT+GGG + chr2_2:18372549-18372568 None:intergenic 50.0%
TTGAAGCAGCTCCACCATGT+TGG + chr2_2:18372550-18372569 None:intergenic 50.0%
!! AGGGAAGCACTTCCTTTGGT+TGG - chr2_2:18372605-18372624 Msa0242270:intron 50.0%
TTAGACGGCTGTCCGATGAG+TGG - chr2_2:18371632-18371651 Msa0242270:intron 55.0%
CCATCAAACGGGTCCTGTGA+AGG + chr2_2:18371772-18371791 None:intergenic 55.0%
CCTTCACAGGACCCGTTTGA+TGG - chr2_2:18371769-18371788 Msa0242270:intron 55.0%
TGAAGGCCTGTTCGATGGTC+AGG + chr2_2:18372000-18372019 None:intergenic 55.0%
TGACCATCGAACAGGCCTTC+AGG - chr2_2:18371999-18372018 Msa0242270:intron 55.0%
GACCATCGAACAGGCCTTCA+GGG - chr2_2:18372000-18372019 Msa0242270:intron 55.0%
AACCCTGAAGGCCTGTTCGA+TGG + chr2_2:18372005-18372024 None:intergenic 55.0%
TTATGGTGGCTACGCGCGAA+AGG - chr2_2:18372325-18372344 Msa0242270:intron 55.0%
TTAGACGGCTGTCCGATGAG+TGG - chr2_2:18371632-18371651 Msa0242270:intron 55.0%
CCATCAAACGGGTCCTGTGA+AGG + chr2_2:18371772-18371791 None:intergenic 55.0%
CCTTCACAGGACCCGTTTGA+TGG - chr2_2:18371769-18371788 Msa0242270:intron 55.0%
TGAAGGCCTGTTCGATGGTC+AGG + chr2_2:18372000-18372019 None:intergenic 55.0%
TGACCATCGAACAGGCCTTC+AGG - chr2_2:18371999-18372018 Msa0242270:intron 55.0%
GACCATCGAACAGGCCTTCA+GGG - chr2_2:18372000-18372019 Msa0242270:intron 55.0%
AACCCTGAAGGCCTGTTCGA+TGG + chr2_2:18372005-18372024 None:intergenic 55.0%
TTATGGTGGCTACGCGCGAA+AGG - chr2_2:18372325-18372344 Msa0242270:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 18371515 18372631 18371515 ID=Msa0242270;Name=Msa0242270
chr2_2 mRNA 18371515 18372631 18371515 ID=Msa0242270-mRNA-1;Parent=Msa0242270;Name=Msa0242270-mRNA-1;_AED=0.47;_eAED=0.48;_QI=0|0|0|1|1|0.66|3|0|72
chr2_2 exon 18372625 18372631 18372625 ID=Msa0242270-mRNA-1:exon:7534;Parent=Msa0242270-mRNA-1
chr2_2 exon 18371800 18371891 18371800 ID=Msa0242270-mRNA-1:exon:7533;Parent=Msa0242270-mRNA-1
chr2_2 exon 18371515 18371631 18371515 ID=Msa0242270-mRNA-1:exon:7532;Parent=Msa0242270-mRNA-1
chr2_2 CDS 18372625 18372631 18372625 ID=Msa0242270-mRNA-1:cds;Parent=Msa0242270-mRNA-1
chr2_2 CDS 18371800 18371891 18371800 ID=Msa0242270-mRNA-1:cds;Parent=Msa0242270-mRNA-1
chr2_2 CDS 18371515 18371631 18371515 ID=Msa0242270-mRNA-1:cds;Parent=Msa0242270-mRNA-1
Gene Sequence

>Msa0242270

ATGGCAGAATACTTGGATCATGAAAATTCAACAGTCAGTACATCTAATCCATCATGTGCGTTAATTAGCGGCATAGCTTATGGTGGCTACGCGCGAAAGATTGTTGGTAGAGTATTGAGATCCATGCAATCCCAACATGGTGGAGCTGCTTCAATTGAAGATCATCCTGAGCATCTCTTATTGGCTCTAAGGGAAGCACTTCCTTTGGTTGGACCT

Protein sequence

>Msa0242270

MAEYLDHENSTVSTSNPSCALISGIAYGGYARKIVGRVLRSMQSQHGGAASIEDHPEHLLLALREALPLVGP