AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0259680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0259680 MtrunA17_Chr2g0333751 98.655 223 3 0 1 223 1 223 2.43e-162 447
Msa0259680 MtrunA17_Chr4g0001201 85.377 212 31 0 12 223 2 213 1.44e-132 371
Msa0259680 MtrunA17_Chr4g0011831 36.481 233 127 6 6 223 14 240 6.90e-42 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0259680 AT1G15880.1 79.452 219 44 1 5 223 6 223 9.32e-125 352
Msa0259680 AT2G45200.1 36.481 233 127 5 6 223 13 239 4.91e-41 140
Msa0259680 AT2G45200.2 33.865 251 127 5 6 223 13 257 3.23e-38 133

Find 65 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTATACTTGTCTATTA+TGG 0.153434 2_2:+56943651 Msa0259680:three_prime_UTR
TGATGGATGCACGCTCTTTA+AGG 0.233741 2_2:-56942771 None:intergenic
GTCTTCCAGCGTTCGACTTT+TGG 0.265092 2_2:+56943153 Msa0259680:CDS
ATTGTATACTTGTCTATTAT+GGG 0.266472 2_2:+56943652 Msa0259680:three_prime_UTR
ACCATTATACTTGCCCTTGT+TGG 0.314904 2_2:+56943538 Msa0259680:CDS
TGATCGAACAAGATTAGATT+TGG 0.341869 2_2:+56942717 Msa0259680:CDS
TGAGATCTTGATAAATTTCT+TGG 0.346054 2_2:-56941725 None:intergenic
CCATTATACTTGCCCTTGTT+GGG 0.360182 2_2:+56943539 Msa0259680:CDS
CAATAAACATCATCTTTACT+TGG 0.366621 2_2:-56943599 None:intergenic
GTAATGCATTGTTTGATTGC+TGG 0.385471 2_2:+56943682 Msa0259680:three_prime_UTR
ATGTGTTACAATTCATAGTT+CGG 0.390079 2_2:+56943781 Msa0259680:three_prime_UTR
TGTTTATTGTGTTGGTAGCT+TGG 0.396853 2_2:+56943613 Msa0259680:three_prime_UTR
GTCCTCTGGTGGGTCTGAAA+TGG 0.402834 2_2:+56941680 Msa0259680:CDS
CCCAACAAGGGCAAGTATAA+TGG 0.403543 2_2:-56943539 None:intergenic
TTGGGATTCCCTTCGCAAAC+AGG 0.411483 2_2:+56939432 Msa0259680:CDS
CAGGCTTGGGTGTCCTCTGG+TGG 0.415447 2_2:+56941669 Msa0259680:CDS
ATCCATGGAGGTTCAAAGTT+GGG 0.434741 2_2:+56939414 Msa0259680:exon
TAAAATATTTGTAGAGTGAA+AGG 0.434967 2_2:-56943845 None:intergenic
ATGCAGGCTTGGGTGTCCTC+TGG 0.438772 2_2:+56941666 Msa0259680:CDS
AGCTCAGTTGGATGAGCAAA+TGG 0.441426 2_2:+56941527 Msa0259680:CDS
TTCACAAATTAATCTTTACT+AGG 0.448626 2_2:-56943727 None:intergenic
AAAATATTTGTAGAGTGAAA+GGG 0.449936 2_2:-56943844 None:intergenic
AACAAGATTAGATTTGGAAG+AGG 0.457292 2_2:+56942723 Msa0259680:CDS
TCAACGAAAGCTTCTACAAA+AGG 0.463049 2_2:+56941570 Msa0259680:CDS
CAGAGAGTGATCTGGAGTCT+TGG 0.464648 2_2:+56941601 Msa0259680:CDS
TGAGGCAACAGAGAGTGATC+TGG 0.471531 2_2:+56941593 Msa0259680:CDS
TAAACTCACAGATGCAGGCT+TGG 0.471681 2_2:+56941655 Msa0259680:CDS
AAACTCACAGATGCAGGCTT+GGG 0.480195 2_2:+56941656 Msa0259680:CDS
CTGTCCTGTACTGCGGCTGA+TGG 0.485234 2_2:-56942788 None:intergenic
AAAGATGATGTTTATTGTGT+TGG 0.505371 2_2:+56943605 Msa0259680:exon
CATCCATGGAGGTTCAAAGT+TGG 0.506812 2_2:+56939413 Msa0259680:exon
ACAAGTAAACTCACAGATGC+AGG 0.514539 2_2:+56941650 Msa0259680:CDS
AGGCTTGGGTGTCCTCTGGT+GGG 0.515681 2_2:+56941670 Msa0259680:CDS
GTCTGTGGCGTGTGAACAGA+CGG 0.519084 2_2:+56939359 Msa0259680:five_prime_UTR
TTGTATACTTGTCTATTATG+GGG 0.525930 2_2:+56943653 Msa0259680:three_prime_UTR
GCATCCATCAGCCGCAGTAC+AGG 0.527799 2_2:+56942784 Msa0259680:CDS
AAGAAAACTTGAAGCTCAGT+TGG 0.529386 2_2:+56941515 Msa0259680:CDS
AGATTAGATTTGGAAGAGGG+TGG 0.530846 2_2:+56942727 Msa0259680:CDS
AGAAATGAAAATTGAAGAAG+CGG 0.531407 2_2:+56939326 Msa0259680:five_prime_UTR
CAACTTTGAACCTCCATGGA+TGG 0.538871 2_2:-56939412 None:intergenic
CATCAGCCGCAGTACAGGAC+AGG 0.540958 2_2:+56942789 Msa0259680:CDS
AGCGGTGTGGTCGTCGTCTG+TGG 0.544478 2_2:+56939344 Msa0259680:five_prime_UTR
ATCCCAACTTTGAACCTCCA+TGG 0.546573 2_2:-56939416 None:intergenic
ATGCATTGTTTGATTGCTGG+TGG 0.554948 2_2:+56943685 Msa0259680:three_prime_UTR
TGTATACTTGTCTATTATGG+GGG 0.555133 2_2:+56943654 Msa0259680:three_prime_UTR
GCTTAGCTCTAAGATTAGAC+CGG 0.556599 2_2:-56942657 None:intergenic
AATTGAAACCTGTTTGCGAA+GGG 0.560013 2_2:-56939440 None:intergenic
ACAAGATTAGATTTGGAAGA+GGG 0.565048 2_2:+56942724 Msa0259680:CDS
AAATTGAAACCTGTTTGCGA+AGG 0.567814 2_2:-56939441 None:intergenic
CTAACCATCTCATCCATCCA+TGG 0.579099 2_2:+56939399 Msa0259680:exon
TCCCACCAAAAGTCGAACGC+TGG 0.611928 2_2:-56943158 None:intergenic
AAATGCATACGGACCCAACA+AGG 0.613983 2_2:-56943552 None:intergenic
CGTGAGCAGTCGCCTCCCAA+CGG 0.616247 2_2:+56943200 Msa0259680:CDS
ACCTCCATGGATGGATGAGA+TGG 0.623902 2_2:-56939403 None:intergenic
GTATACTTGTCTATTATGGG+GGG 0.624761 2_2:+56943655 Msa0259680:three_prime_UTR
TGGATGCACGCTCTTTAAGG+AGG 0.630188 2_2:-56942768 None:intergenic
TCACAAGCACAAGCAACACT+CGG 0.631705 2_2:+56943123 Msa0259680:CDS
TGAAAATTGAAGAAGCGGTG+TGG 0.662471 2_2:+56939331 Msa0259680:five_prime_UTR
GAAAGCTTCTACAAAAGGTG+AGG 0.666826 2_2:+56941575 Msa0259680:CDS
AATGCATACGGACCCAACAA+GGG 0.667761 2_2:-56943551 None:intergenic
ACAGCAATCATACCGTTGGG+AGG 0.674625 2_2:-56943212 None:intergenic
AACCATTTCAGACCCACCAG+AGG 0.691979 2_2:-56941682 None:intergenic
TAACAACCTGTCCTGTACTG+CGG 0.715310 2_2:-56942795 None:intergenic
ACCATCTCATCCATCCATGG+AGG 0.728976 2_2:+56939402 Msa0259680:exon
AATTTATCAAGATCTCACCC+AGG 0.754080 2_2:+56941731 Msa0259680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATATTTTATGTTTTTAAA+TGG + chr2_2:56939286-56939305 Msa0259680:five_prime_UTR 10.0%
!!! TCATTTTTTCAAATAATTAT+TGG - chr2_2:56939775-56939794 None:intergenic 10.0%
!! ATATTTAATGATAAATGAAA+AGG - chr2_2:56941927-56941946 None:intergenic 10.0%
!! TATAATAAATAATGTTATAG+GGG - chr2_2:56942421-56942440 None:intergenic 10.0%
!! GTTATAATAAATAATGTTAT+AGG - chr2_2:56942423-56942442 None:intergenic 10.0%
!!! CAAAAATATTTGAAATATAA+AGG - chr2_2:56942570-56942589 None:intergenic 10.0%
!!! AAGCTTATATATAATTTTAT+AGG - chr2_2:56942919-56942938 None:intergenic 10.0%
!! GTTTAAATAAATATTGAATA+AGG + chr2_2:56943239-56943258 Msa0259680:intron 10.0%
!! TAAAATTCAAAACTATGATA+TGG + chr2_2:56939500-56939519 Msa0259680:intron 15.0%
!! AAAATTCAAAACTATGATAT+GGG + chr2_2:56939501-56939520 Msa0259680:intron 15.0%
!!! TTTATCACTTTTTAATCTAA+TGG + chr2_2:56940005-56940024 Msa0259680:intron 15.0%
!!! TATGTTCTTTTTGTTATTTT+GGG + chr2_2:56940632-56940651 Msa0259680:intron 15.0%
!!! TTTTTTTTTTTCTTTCTTGA+AGG + chr2_2:56941388-56941407 Msa0259680:intron 15.0%
!!! TTTTTTTTTTCTTTCTTGAA+GGG + chr2_2:56941389-56941408 Msa0259680:intron 15.0%
!! GTAACAGTTATAATAAATAA+TGG - chr2_2:56942467-56942486 None:intergenic 15.0%
!!! TTAATGTTTTATTTGGTTGT+CGG + chr2_2:56939565-56939584 Msa0259680:intron 20.0%
!! TGATATATGATAGAACATTA+TGG - chr2_2:56940295-56940314 None:intergenic 20.0%
!!! CTATGTTCTTTTTGTTATTT+TGG + chr2_2:56940631-56940650 Msa0259680:intron 20.0%
!!! AAAAACTTGCTTTTGAAAAA+GGG - chr2_2:56940826-56940845 None:intergenic 20.0%
!!! TAAGCAAAAAACTACTTTTA+AGG - chr2_2:56940989-56941008 None:intergenic 20.0%
!!! TCTTTTTATCTGTATTATGA+CGG - chr2_2:56941177-56941196 None:intergenic 20.0%
!!! ATCAAGTAATTTTGTTGAAT+TGG + chr2_2:56941364-56941383 Msa0259680:intron 20.0%
!!! TCAAGTAATTTTGTTGAATT+GGG + chr2_2:56941365-56941384 Msa0259680:intron 20.0%
!!! AATGGTTTTATATCGTAAAT+TGG + chr2_2:56941545-56941564 Msa0259680:CDS 20.0%
!! AGGTCAAGTATAAATTTATT+AGG - chr2_2:56941964-56941983 None:intergenic 20.0%
!!! TGCAATTTGTGTTTTAAAAA+AGG - chr2_2:56942091-56942110 None:intergenic 20.0%
!! ACATTGGAATTGAAATAAAA+GGG - chr2_2:56942359-56942378 None:intergenic 20.0%
!! TTTAAATACATCTAACAAGT+TGG - chr2_2:56942617-56942636 None:intergenic 20.0%
!! GAAAATAAGAAAAATGCATA+CGG - chr2_2:56943566-56943585 None:intergenic 20.0%
!!! TTTTTCTTATTTTCATCTAC+TGG + chr2_2:56943572-56943591 Msa0259680:CDS 20.0%
!! TATTGTATACTTGTCTATTA+TGG + chr2_2:56943651-56943670 Msa0259680:three_prime_UTR 20.0%
!! ATTGTATACTTGTCTATTAT+GGG + chr2_2:56943652-56943671 Msa0259680:three_prime_UTR 20.0%
!! TTCACAAATTAATCTTTACT+AGG - chr2_2:56943730-56943749 None:intergenic 20.0%
! AGAAATGAAAATTGAAGAAG+CGG + chr2_2:56939326-56939345 Msa0259680:five_prime_UTR 25.0%
! ACCTATAAACCATATCAAAA+GGG - chr2_2:56939619-56939638 None:intergenic 25.0%
! AACCTATAAACCATATCAAA+AGG - chr2_2:56939620-56939639 None:intergenic 25.0%
!!! TATAGTTTTGAGTGATGAAT+TGG + chr2_2:56939671-56939690 Msa0259680:intron 25.0%
!!! ATGACATGATTTTGACATTT+TGG + chr2_2:56939695-56939714 Msa0259680:intron 25.0%
!!! TGACATGATTTTGACATTTT+GGG + chr2_2:56939696-56939715 Msa0259680:intron 25.0%
! AAACAGTTTGAATTCTATAG+TGG - chr2_2:56939988-56940007 None:intergenic 25.0%
! CATAATACTATATACACCAA+AGG - chr2_2:56940516-56940535 None:intergenic 25.0%
! CATATACTAATAGGAAACAA+AGG - chr2_2:56940493-56940512 None:intergenic 25.0%
! CATAATACTATATACACCAA+AGG - chr2_2:56940516-56940535 None:intergenic 25.0%
!!! CAAAAACTTGCTTTTGAAAA+AGG - chr2_2:56940827-56940846 None:intergenic 25.0%
!! TTTTTGTAGAATCTCTATCA+CGG + chr2_2:56940841-56940860 Msa0259680:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr2_2:56940999-56941018 None:intergenic 25.0%
!!! TAAGCAAAAAACTACTTTTG+AGG + chr2_2:56940900-56940919 Msa0259680:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + chr2_2:56940999-56941018 Msa0259680:intron 25.0%
!!! ATTATTTTAAATACTCCCTC+CGG - chr2_2:56941021-56941040 None:intergenic 25.0%
! AGATCAAATAATCAAGATGA+CGG + chr2_2:56941057-56941076 Msa0259680:intron 25.0%
! AAAAGACGACTTAAAGTTTA+TGG + chr2_2:56941191-56941210 Msa0259680:intron 25.0%
!!! GCATAGTTCAAGTTAATTTT+AGG + chr2_2:56941414-56941433 Msa0259680:intron 25.0%
! TGAGATCTTGATAAATTTCT+TGG - chr2_2:56941728-56941747 None:intergenic 25.0%
! CATATTATATCATGTAGTTG+TGG + chr2_2:56941802-56941821 Msa0259680:intron 25.0%
!! TTTAGTTTAAATAAGAGTGG+GGG - chr2_2:56942230-56942249 None:intergenic 25.0%
!! GTTTAGTTTAAATAAGAGTG+GGG - chr2_2:56942231-56942250 None:intergenic 25.0%
!! GGTTTAGTTTAAATAAGAGT+GGG - chr2_2:56942232-56942251 None:intergenic 25.0%
!! AGGTTTAGTTTAAATAAGAG+TGG - chr2_2:56942233-56942252 None:intergenic 25.0%
! GACATTGGAATTGAAATAAA+AGG - chr2_2:56942360-56942379 None:intergenic 25.0%
! TTTCAATTCCAATGTCTTAT+AGG + chr2_2:56942364-56942383 Msa0259680:intron 25.0%
!! GTTAGATGTATTTAAAATGC+AGG + chr2_2:56942621-56942640 Msa0259680:intron 25.0%
!!! TTAAAATGCAGGAGTTTTAT+CGG + chr2_2:56942632-56942651 Msa0259680:intron 25.0%
! CAAATGTCTGATATTAAAGA+TGG - chr2_2:56942971-56942990 None:intergenic 25.0%
! GAAAAAATAATCCATTAGCT+TGG - chr2_2:56942994-56943013 None:intergenic 25.0%
! AAACTTCAAATGAACATTCA+AGG - chr2_2:56943016-56943035 None:intergenic 25.0%
! TAAAACTTGTGTAGTAGAAA+TGG - chr2_2:56943427-56943446 None:intergenic 25.0%
!!! ACACAAGTTTTATTTCTCTA+TGG + chr2_2:56943435-56943454 Msa0259680:intron 25.0%
! CAATAAACATCATCTTTACT+TGG - chr2_2:56943602-56943621 None:intergenic 25.0%
! AAAGATGATGTTTATTGTGT+TGG + chr2_2:56943605-56943624 Msa0259680:exon 25.0%
! TTGTATACTTGTCTATTATG+GGG + chr2_2:56943653-56943672 Msa0259680:three_prime_UTR 25.0%
! ATGTGTTACAATTCATAGTT+CGG + chr2_2:56943781-56943800 Msa0259680:three_prime_UTR 25.0%
! ACCCTTTTGATATGGTTTAT+AGG + chr2_2:56939615-56939634 Msa0259680:intron 30.0%
!!! TATAGGTTTTGAGAATTTCC+TGG + chr2_2:56939632-56939651 Msa0259680:intron 30.0%
TACTATCACTATACAAAACC+AGG - chr2_2:56939653-56939672 None:intergenic 30.0%
TAGGATCAATGATCTATGAA+GGG + chr2_2:56939725-56939744 Msa0259680:intron 30.0%
!! TTTGACTAGTTTCTGATGAT+AGG + chr2_2:56939863-56939882 Msa0259680:intron 30.0%
!! TTGACTAGTTTCTGATGATA+GGG + chr2_2:56939864-56939883 Msa0259680:intron 30.0%
TGCTTAGAAAACAAGTTTGT+AGG + chr2_2:56939928-56939947 Msa0259680:intron 30.0%
! ATATTCTGATGTTTTACGTG+TGG + chr2_2:56940070-56940089 Msa0259680:intron 30.0%
TGTTAGACTTTCATATGACA+AGG - chr2_2:56940153-56940172 None:intergenic 30.0%
ATATGAAAGTCTAACATGAG+AGG + chr2_2:56940157-56940176 Msa0259680:intron 30.0%
TCAATTTCTGCATATGCATT+CGG + chr2_2:56940384-56940403 Msa0259680:intron 30.0%
ACCTGGATAAAAGATTGATT+CGG + chr2_2:56940412-56940431 Msa0259680:intron 30.0%
TTCCTATTAGTATATGCCTT+TGG + chr2_2:56940497-56940516 Msa0259680:intron 30.0%
TTCCTATTAGTATATGCCTT+TGG + chr2_2:56940497-56940516 Msa0259680:intron 30.0%
CATTGAATGAATGATGTATG+TGG + chr2_2:56940925-56940944 Msa0259680:intron 30.0%
TAGCAAAAACCTGTATAACT+TGG + chr2_2:56941285-56941304 Msa0259680:intron 30.0%
ACAGAAAAACCAAGTTATAC+AGG - chr2_2:56941297-56941316 None:intergenic 30.0%
! TTGTCTTTTTAACCTTTTGC+AGG + chr2_2:56941491-56941510 Msa0259680:intron 30.0%
TATGCTCACACATAATGATT+GGG - chr2_2:56941786-56941805 None:intergenic 30.0%
ATATGCTCACACATAATGAT+TGG - chr2_2:56941787-56941806 None:intergenic 30.0%
ATAAAGAACCCTTTACATCA+GGG - chr2_2:56941869-56941888 None:intergenic 30.0%
!!! GAGAATTCTTTTGAAACTGA+AGG - chr2_2:56941984-56942003 None:intergenic 30.0%
TCAACTTCAGAATCAAAACA+GGG - chr2_2:56942126-56942145 None:intergenic 30.0%
AATTGCCAGTTAGAACAATT+AGG - chr2_2:56942277-56942296 None:intergenic 30.0%
TTGGAATTGAAATAAAAGGG+TGG - chr2_2:56942356-56942375 None:intergenic 30.0%
AATGACATCCTATAAGACAT+TGG - chr2_2:56942375-56942394 None:intergenic 30.0%
! ACTTTTTAATCTTCTCCATC+AGG + chr2_2:56942489-56942508 Msa0259680:intron 30.0%
TGATCGAACAAGATTAGATT+TGG + chr2_2:56942717-56942736 Msa0259680:CDS 30.0%
AACAAGATTAGATTTGGAAG+AGG + chr2_2:56942723-56942742 Msa0259680:CDS 30.0%
ACAAGATTAGATTTGGAAGA+GGG + chr2_2:56942724-56942743 Msa0259680:CDS 30.0%
! TTTTATCAAGATTGTTCCAC+TGG + chr2_2:56942852-56942871 Msa0259680:intron 30.0%
TCTGATATTAAAGATGGCAT+TGG - chr2_2:56942965-56942984 None:intergenic 30.0%
!!! CTGAAGTTTTTTTTTTGTCC+AGG + chr2_2:56943386-56943405 Msa0259680:intron 30.0%
TGGAATATTAGTTATTCTCC+TGG - chr2_2:56943407-56943426 None:intergenic 30.0%
CTATGGTGTTTGTTTAATGA+CGG + chr2_2:56943452-56943471 Msa0259680:intron 30.0%
AATAAAGCGAAAAAAGTCGA+TGG + chr2_2:56943513-56943532 Msa0259680:CDS 30.0%
TGTATACTTGTCTATTATGG+GGG + chr2_2:56943654-56943673 Msa0259680:three_prime_UTR 30.0%
AATTGAAACCTGTTTGCGAA+GGG - chr2_2:56939443-56939462 None:intergenic 35.0%
AAATTGAAACCTGTTTGCGA+AGG - chr2_2:56939444-56939463 None:intergenic 35.0%
TATGGGTTTGTGTAATTCAG+AGG + chr2_2:56939518-56939537 Msa0259680:intron 35.0%
!! TTTTATTTGGTTGTCGGTGA+TGG + chr2_2:56939571-56939590 Msa0259680:intron 35.0%
!!! TTGTCGGTGATGGTTTTTTT+CGG + chr2_2:56939581-56939600 Msa0259680:intron 35.0%
! GAGTTTCTACCCTTTTGATA+TGG + chr2_2:56939607-56939626 Msa0259680:intron 35.0%
!!! TTGACATTTTGGGTTCATCT+AGG + chr2_2:56939706-56939725 Msa0259680:intron 35.0%
CTAGGATCAATGATCTATGA+AGG + chr2_2:56939724-56939743 Msa0259680:intron 35.0%
CAGAAACTAGTCAAACTACA+AGG - chr2_2:56939858-56939877 None:intergenic 35.0%
!! TGACTAGTTTCTGATGATAG+GGG + chr2_2:56939865-56939884 Msa0259680:intron 35.0%
GGGCTATAAATTCTGATCTT+TGG + chr2_2:56939885-56939904 Msa0259680:intron 35.0%
AACATGAGAGGATTCATTCT+CGG + chr2_2:56940169-56940188 Msa0259680:intron 35.0%
TGTTTCTTGCATCTTACAAC+CGG - chr2_2:56940206-56940225 None:intergenic 35.0%
TGCATATACAGCAACATAAG+TGG + chr2_2:56940244-56940263 Msa0259680:intron 35.0%
GCATATACAGCAACATAAGT+GGG + chr2_2:56940245-56940264 Msa0259680:intron 35.0%
CATATACAGCAACATAAGTG+GGG + chr2_2:56940246-56940265 Msa0259680:intron 35.0%
ATTGATGTACAAATCACTCG+AGG - chr2_2:56940369-56940388 None:intergenic 35.0%
! TCCGAATCAATCTTTTATCC+AGG - chr2_2:56940416-56940435 None:intergenic 35.0%
! CACCAAAGGCATATACTAAT+AGG - chr2_2:56940502-56940521 None:intergenic 35.0%
! CACCAAAGGCATATACTAAT+AGG - chr2_2:56940502-56940521 None:intergenic 35.0%
! TCTTGTTAAACCTTTTCCCA+CGG + chr2_2:56940600-56940619 Msa0259680:intron 35.0%
TCATAACTCATACAGTCATC+AGG - chr2_2:56940736-56940755 None:intergenic 35.0%
CATTGAATGAGTGATGTATG+TGG - chr2_2:56940964-56940983 None:intergenic 35.0%
AATCATCTTGATCAGACTTC+TGG + chr2_2:56941112-56941131 Msa0259680:intron 35.0%
!! ATCTGTATTATGACGGACTA+CGG - chr2_2:56941170-56941189 None:intergenic 35.0%
AAGAAAACTTGAAGCTCAGT+TGG + chr2_2:56941515-56941534 Msa0259680:CDS 35.0%
TCAACGAAAGCTTCTACAAA+AGG + chr2_2:56941570-56941589 Msa0259680:CDS 35.0%
AATTTATCAAGATCTCACCC+AGG + chr2_2:56941731-56941750 Msa0259680:CDS 35.0%
ATGCTCACACATAATGATTG+GGG - chr2_2:56941785-56941804 None:intergenic 35.0%
CATAAAGAACCCTTTACATC+AGG - chr2_2:56941870-56941889 None:intergenic 35.0%
GTTCTTTATGATAGCTGAGA+AGG + chr2_2:56941880-56941899 Msa0259680:intron 35.0%
GTCAACTTCAGAATCAAAAC+AGG - chr2_2:56942127-56942146 None:intergenic 35.0%
AGTCACCTAATTGTTCTAAC+TGG + chr2_2:56942269-56942288 Msa0259680:intron 35.0%
! TATTAAAGATGGCATTGGAG+CGG - chr2_2:56942960-56942979 None:intergenic 35.0%
GTTTATTCTTGTACTGCAGA+TGG + chr2_2:56943089-56943108 Msa0259680:intron 35.0%
!! TGTTTATTGTGTTGGTAGCT+TGG + chr2_2:56943613-56943632 Msa0259680:three_prime_UTR 35.0%
GTATACTTGTCTATTATGGG+GGG + chr2_2:56943655-56943674 Msa0259680:three_prime_UTR 35.0%
GTAATGCATTGTTTGATTGC+TGG + chr2_2:56943682-56943701 Msa0259680:three_prime_UTR 35.0%
TGAAAATTGAAGAAGCGGTG+TGG + chr2_2:56939331-56939350 Msa0259680:five_prime_UTR 40.0%
ATCCATGGAGGTTCAAAGTT+GGG + chr2_2:56939414-56939433 Msa0259680:exon 40.0%
!!! TGTCGGTGATGGTTTTTTTC+GGG + chr2_2:56939582-56939601 Msa0259680:intron 40.0%
TTTCTGATCATAGGTGTGTC+AGG - chr2_2:56939808-56939827 None:intergenic 40.0%
! GCACTGACATTTCTGATCAT+AGG - chr2_2:56939817-56939836 None:intergenic 40.0%
! TTCTGATGTTTTACGTGTGG+TGG + chr2_2:56940073-56940092 Msa0259680:intron 40.0%
! ATGTTTTACGTGTGGTGGTA+TGG + chr2_2:56940078-56940097 Msa0259680:intron 40.0%
TTTCATATGACAAGGTGAGG+TGG - chr2_2:56940145-56940164 None:intergenic 40.0%
GACTTTCATATGACAAGGTG+AGG - chr2_2:56940148-56940167 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr2_2:56941003-56941022 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr2_2:56941002-56941021 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr2_2:56941002-56941021 Msa0259680:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr2_2:56941003-56941022 Msa0259680:intron 40.0%
! AATCAAGATGACGGCAGTAT+TGG + chr2_2:56941066-56941085 Msa0259680:intron 40.0%
! CAAGTTTTCTTGCCTGCAAA+AGG - chr2_2:56941506-56941525 None:intergenic 40.0%
GAAAGCTTCTACAAAAGGTG+AGG + chr2_2:56941575-56941594 Msa0259680:CDS 40.0%
ACAAGTAAACTCACAGATGC+AGG + chr2_2:56941650-56941669 Msa0259680:CDS 40.0%
GCTCATACGATGTAATACCT+GGG - chr2_2:56941751-56941770 None:intergenic 40.0%
AGCTCATACGATGTAATACC+TGG - chr2_2:56941752-56941771 None:intergenic 40.0%
! AGGGAGTACAGTTTGCAAAT+AGG - chr2_2:56941850-56941869 None:intergenic 40.0%
AACTGTACTCCCTGATGTAA+AGG + chr2_2:56941857-56941876 Msa0259680:intron 40.0%
ACTGTACTCCCTGATGTAAA+GGG + chr2_2:56941858-56941877 Msa0259680:intron 40.0%
! CTCTTTTCTACAGATGAGAG+AGG + chr2_2:56942001-56942020 Msa0259680:intron 40.0%
! ATGAGAGAGGAGTATAACAG+AGG + chr2_2:56942014-56942033 Msa0259680:intron 40.0%
! TGAGAGAGGAGTATAACAGA+GGG + chr2_2:56942015-56942034 Msa0259680:intron 40.0%
!! GGAGTCTTTCTGAAGCTAAA+AGG - chr2_2:56942070-56942089 None:intergenic 40.0%
ACTTCAGAATCAAAACAGGG+TGG - chr2_2:56942123-56942142 None:intergenic 40.0%
!! AGTTCTGAAAAACAGAGACG+TGG - chr2_2:56942179-56942198 None:intergenic 40.0%
AAATAAGAGTGGGGGTAGAA+GGG - chr2_2:56942222-56942241 None:intergenic 40.0%
TAAATAAGAGTGGGGGTAGA+AGG - chr2_2:56942223-56942242 None:intergenic 40.0%
ACTAAACCTTCTAACCTCTC+TGG + chr2_2:56942244-56942263 Msa0259680:intron 40.0%
ACATCACAAAGCAACAGAGT+AGG - chr2_2:56942515-56942534 None:intergenic 40.0%
!! CTCTGTTGCTTTGTGATGTT+AGG + chr2_2:56942516-56942535 Msa0259680:intron 40.0%
TGTCTGACAGATCTAAGCTT+AGG - chr2_2:56942594-56942613 None:intergenic 40.0%
!! GCTTAGCTCTAAGATTAGAC+CGG - chr2_2:56942660-56942679 None:intergenic 40.0%
AGATTAGATTTGGAAGAGGG+TGG + chr2_2:56942727-56942746 Msa0259680:CDS 40.0%
TCAGACATTTGCCAAGCTAA+TGG + chr2_2:56942980-56942999 Msa0259680:intron 40.0%
TCAACAGCAATCATACCGTT+GGG - chr2_2:56943218-56943237 None:intergenic 40.0%
TTCAACAGCAATCATACCGT+TGG - chr2_2:56943219-56943238 None:intergenic 40.0%
! ACGGCTTTTAACAATGTTGC+AGG + chr2_2:56943471-56943490 Msa0259680:intron 40.0%
ACCATTATACTTGCCCTTGT+TGG + chr2_2:56943538-56943557 Msa0259680:CDS 40.0%
CCATTATACTTGCCCTTGTT+GGG + chr2_2:56943539-56943558 Msa0259680:CDS 40.0%
ATGCATTGTTTGATTGCTGG+TGG + chr2_2:56943685-56943704 Msa0259680:three_prime_UTR 40.0%
CTAACCATCTCATCCATCCA+TGG + chr2_2:56939399-56939418 Msa0259680:exon 45.0%
CAACTTTGAACCTCCATGGA+TGG - chr2_2:56939415-56939434 None:intergenic 45.0%
CATCCATGGAGGTTCAAAGT+TGG + chr2_2:56939413-56939432 Msa0259680:exon 45.0%
ATCCCAACTTTGAACCTCCA+TGG - chr2_2:56939419-56939438 None:intergenic 45.0%
GAATTCTATAGTGGCTGCAC+AGG - chr2_2:56939979-56939998 None:intergenic 45.0%
ATGAGAGGATTCATTCTCGG+TGG + chr2_2:56940172-56940191 Msa0259680:intron 45.0%
TGAGAGGATTCATTCTCGGT+GGG + chr2_2:56940173-56940192 Msa0259680:intron 45.0%
ATATGCATTCGGCGATAACC+TGG + chr2_2:56940395-56940414 Msa0259680:intron 45.0%
! TAAACCTTTTCCCACGGCAT+AGG + chr2_2:56940606-56940625 Msa0259680:intron 45.0%
! AAACCTTTTCCCACGGCATA+GGG + chr2_2:56940607-56940626 Msa0259680:intron 45.0%
AGAATCTCTATCACGGCTTC+TGG + chr2_2:56940848-56940867 Msa0259680:intron 45.0%
TGGCTAATACTTACTCCCTC+CGG + chr2_2:56940868-56940887 Msa0259680:intron 45.0%
AGCTCAGTTGGATGAGCAAA+TGG + chr2_2:56941527-56941546 Msa0259680:CDS 45.0%
TAAACTCACAGATGCAGGCT+TGG + chr2_2:56941655-56941674 Msa0259680:CDS 45.0%
AAACTCACAGATGCAGGCTT+GGG + chr2_2:56941656-56941675 Msa0259680:CDS 45.0%
! GAGAGAGGAGTATAACAGAG+GGG + chr2_2:56942016-56942035 Msa0259680:intron 45.0%
TCAAAACAGGGTGGACGAAT+AGG - chr2_2:56942114-56942133 None:intergenic 45.0%
! AATTAGGTGACTAGCCAGAG+AGG - chr2_2:56942261-56942280 None:intergenic 45.0%
! TGATGGATGCACGCTCTTTA+AGG - chr2_2:56942774-56942793 None:intergenic 45.0%
TAACAACCTGTCCTGTACTG+CGG - chr2_2:56942798-56942817 None:intergenic 45.0%
TAATAAGTGACGCACTCCAG+TGG - chr2_2:56942871-56942890 None:intergenic 45.0%
TCACAAGCACAAGCAACACT+CGG + chr2_2:56943123-56943142 Msa0259680:CDS 45.0%
CCCAACAAGGGCAAGTATAA+TGG - chr2_2:56943542-56943561 None:intergenic 45.0%
AATGCATACGGACCCAACAA+GGG - chr2_2:56943554-56943573 None:intergenic 45.0%
AAATGCATACGGACCCAACA+AGG - chr2_2:56943555-56943574 None:intergenic 45.0%
TACTTGTCTATTATGGGGGG+AGG + chr2_2:56943658-56943677 Msa0259680:three_prime_UTR 45.0%
ACTTGTCTATTATGGGGGGA+GGG + chr2_2:56943659-56943678 Msa0259680:three_prime_UTR 45.0%
!!! TATTTTTTTAATGTTTTATT+TGG + chr2_2:56939558-56939577 Msa0259680:intron 5.0%
!!! AATATATTTTTTTGTTTTTT+AGG + chr2_2:56941447-56941466 Msa0259680:intron 5.0%
!! TTATAATAAATAATGTTATA+GGG - chr2_2:56942422-56942441 None:intergenic 5.0%
!! TTATTATTTACTTATATATT+TGG + chr2_2:56942885-56942904 Msa0259680:intron 5.0%
ACCATCTCATCCATCCATGG+AGG + chr2_2:56939402-56939421 Msa0259680:exon 50.0%
ACCTCCATGGATGGATGAGA+TGG - chr2_2:56939406-56939425 None:intergenic 50.0%
TTGGGATTCCCTTCGCAAAC+AGG + chr2_2:56939432-56939451 Msa0259680:CDS 50.0%
GTGTGGTGGTATGGTACAGA+CGG + chr2_2:56940087-56940106 Msa0259680:intron 50.0%
ATTCTCGGTGGGTGACTTAC+CGG + chr2_2:56940184-56940203 Msa0259680:intron 50.0%
CATAAGTGGGGTTGACTGCA+TGG + chr2_2:56940258-56940277 Msa0259680:intron 50.0%
GTACAAATCACTCGAGGCAG+AGG - chr2_2:56940363-56940382 None:intergenic 50.0%
GAACATAGACCCTATGCCGT+GGG - chr2_2:56940619-56940638 None:intergenic 50.0%
AGAACATAGACCCTATGCCG+TGG - chr2_2:56940620-56940639 None:intergenic 50.0%
TGAGGCAACAGAGAGTGATC+TGG + chr2_2:56941593-56941612 Msa0259680:CDS 50.0%
! CAGAGAGTGATCTGGAGTCT+TGG + chr2_2:56941601-56941620 Msa0259680:CDS 50.0%
AACCATTTCAGACCCACCAG+AGG - chr2_2:56941685-56941704 None:intergenic 50.0%
AGACGTGGATCAACTGCAAC+GGG - chr2_2:56942164-56942183 None:intergenic 50.0%
GAGACGTGGATCAACTGCAA+CGG - chr2_2:56942165-56942184 None:intergenic 50.0%
! GACTAGCCAGAGAGGTTAGA+AGG - chr2_2:56942253-56942272 None:intergenic 50.0%
AAGCAACAGAGTAGGCCTGA+TGG - chr2_2:56942507-56942526 None:intergenic 50.0%
!!! TGCAGGAGTTTTATCGGCTC+CGG + chr2_2:56942638-56942657 Msa0259680:intron 50.0%
! TGGATGCACGCTCTTTAAGG+AGG - chr2_2:56942771-56942790 None:intergenic 50.0%
ACAGATGTCTGATCCACAGC+TGG + chr2_2:56943042-56943061 Msa0259680:intron 50.0%
CAGATGTCTGATCCACAGCT+GGG + chr2_2:56943043-56943062 Msa0259680:intron 50.0%
AGATGTCTGATCCACAGCTG+GGG + chr2_2:56943044-56943063 Msa0259680:intron 50.0%
AAACAGAACAACCCCAGCTG+TGG - chr2_2:56943058-56943077 None:intergenic 50.0%
! GTCTTCCAGCGTTCGACTTT+TGG + chr2_2:56943153-56943172 Msa0259680:CDS 50.0%
! TTCCAGCGTTCGACTTTTGG+TGG + chr2_2:56943156-56943175 Msa0259680:CDS 50.0%
! TCCAGCGTTCGACTTTTGGT+GGG + chr2_2:56943157-56943176 Msa0259680:CDS 50.0%
ACAGCAATCATACCGTTGGG+AGG - chr2_2:56943215-56943234 None:intergenic 50.0%
CTTGTCTATTATGGGGGGAG+GGG + chr2_2:56943660-56943679 Msa0259680:three_prime_UTR 50.0%
GTCTGTGGCGTGTGAACAGA+CGG + chr2_2:56939359-56939378 Msa0259680:five_prime_UTR 55.0%
TGGCTCAGCAGTGACTGCTT+TGG + chr2_2:56940025-56940044 Msa0259680:intron 55.0%
ACTCGAGGCAGAGGAAGACA+TGG - chr2_2:56940354-56940373 None:intergenic 55.0%
AGACCCTATGCCGTGGGAAA+AGG - chr2_2:56940613-56940632 None:intergenic 55.0%
! GTCCTCTGGTGGGTCTGAAA+TGG + chr2_2:56941680-56941699 Msa0259680:CDS 55.0%
CAACTGCAACGGGAGATCGA+CGG - chr2_2:56942154-56942173 None:intergenic 55.0%
TCCCACCAAAAGTCGAACGC+TGG - chr2_2:56943161-56943180 None:intergenic 55.0%
ATGCAGGCTTGGGTGTCCTC+TGG + chr2_2:56941666-56941685 Msa0259680:CDS 60.0%
AGGCTTGGGTGTCCTCTGGT+GGG + chr2_2:56941670-56941689 Msa0259680:CDS 60.0%
GCATCCATCAGCCGCAGTAC+AGG + chr2_2:56942784-56942803 Msa0259680:CDS 60.0%
CTGTCCTGTACTGCGGCTGA+TGG - chr2_2:56942791-56942810 None:intergenic 60.0%
CATCAGCCGCAGTACAGGAC+AGG + chr2_2:56942789-56942808 Msa0259680:CDS 60.0%
AGCGGTGTGGTCGTCGTCTG+TGG + chr2_2:56939344-56939363 Msa0259680:five_prime_UTR 65.0%
CAGGCTTGGGTGTCCTCTGG+TGG + chr2_2:56941669-56941688 Msa0259680:CDS 65.0%
CGTGAGCAGTCGCCTCCCAA+CGG + chr2_2:56943200-56943219 Msa0259680:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 56939283 56943857 56939283 ID=Msa0259680;Name=Msa0259680
chr2_2 mRNA 56939283 56943857 56939283 ID=Msa0259680-mRNA-1;Parent=Msa0259680;Name=Msa0259680-mRNA-1;_AED=0.04;_eAED=0.04;_QI=135|1|1|1|1|1|5|251|223
chr2_2 exon 56939283 56939453 56939283 ID=Msa0259680-mRNA-1:exon:17220;Parent=Msa0259680-mRNA-1
chr2_2 exon 56941513 56941752 56941513 ID=Msa0259680-mRNA-1:exon:17221;Parent=Msa0259680-mRNA-1
chr2_2 exon 56942643 56942810 56942643 ID=Msa0259680-mRNA-1:exon:17222;Parent=Msa0259680-mRNA-1
chr2_2 exon 56943108 56943221 56943108 ID=Msa0259680-mRNA-1:exon:17223;Parent=Msa0259680-mRNA-1
chr2_2 exon 56943493 56943857 56943493 ID=Msa0259680-mRNA-1:exon:17224;Parent=Msa0259680-mRNA-1
chr2_2 five_prime_UTR 56939283 56939417 56939283 ID=Msa0259680-mRNA-1:five_prime_utr;Parent=Msa0259680-mRNA-1
chr2_2 CDS 56939418 56939453 56939418 ID=Msa0259680-mRNA-1:cds;Parent=Msa0259680-mRNA-1
chr2_2 CDS 56941513 56941752 56941513 ID=Msa0259680-mRNA-1:cds;Parent=Msa0259680-mRNA-1
chr2_2 CDS 56942643 56942810 56942643 ID=Msa0259680-mRNA-1:cds;Parent=Msa0259680-mRNA-1
chr2_2 CDS 56943108 56943221 56943108 ID=Msa0259680-mRNA-1:cds;Parent=Msa0259680-mRNA-1
chr2_2 CDS 56943493 56943606 56943493 ID=Msa0259680-mRNA-1:cds;Parent=Msa0259680-mRNA-1
chr2_2 three_prime_UTR 56943607 56943857 56943607 ID=Msa0259680-mRNA-1:three_prime_utr;Parent=Msa0259680-mRNA-1
Gene Sequence

>Msa0259680

ATGGAGGTTCAAAGTTGGGATTCCCTTCGCAAACAGGCAAGAAAACTTGAAGCTCAGTTGGATGAGCAAATGGTTTTATATCGTAAATTGGTTTCAACGAAAGCTTCTACAAAAGGTGAGGCAACAGAGAGTGATCTGGAGTCTTGGATTGAACGTTTACTTAATCAACTCAAACAAGTAAACTCACAGATGCAGGCTTGGGTGTCCTCTGGTGGGTCTGAAATGGTTTCTCATACACTGACTAGACACCAAGAAATTTATCAAGATCTCACCCAGGAGTTTTATCGGCTCCGGTCTAATCTTAGAGCTAAGCAAGAACACGCTTCACTACTTGATGATTTTAAAGAGCTTGATCGAACAAGATTAGATTTGGAAGAGGGTGGAAGTTCTGAACAGCAAAACCTCCTTAAAGAGCGTGCATCCATCAGCCGCAGTACAGGACAGATGGACACTGTTATTTCACAAGCACAAGCAACACTCGGTGCACTAGTCTTCCAGCGTTCGACTTTTGGTGGGATCAATTCAAAGCTCAGCAACGTGAGCAGTCGCCTCCCAACGGTAAATACTATTCTGTCAGCAATAAAGCGAAAAAAGTCGATGGATACCATTATACTTGCCCTTGTTGGGTCCGTATGCATTTTTCTTATTTTCATCTACTGGCTAACCAAGTAA
Protein sequence

>Msa0259680

MEVQSWDSLRKQARKLEAQLDEQMVLYRKLVSTKASTKGEATESDLESWIERLLNQLKQVNSQMQAWVSSGGSEMVSHTLTRHQEIYQDLTQEFYRLRSNLRAKQEHASLLDDFKELDRTRLDLEEGGSSEQQNLLKERASISRSTGQMDTVISQAQATLGALVFQRSTFGGINSKLSNVSSRLPTVNTILSAIKRKKSMDTIILALVGSVCIFLIFIYWLTK*