AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1410550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1410550 MtrunA17_Chr4g0063471 95.146 103 2 1 1 100 1 103 9.92e-67 195
Msa1410550 MtrunA17_Chr4g0003631 55.319 94 30 4 1 83 1 93 1.76e-24 89.0
Msa1410550 MtrunA17_Chr2g0332771 40.496 121 46 4 1 98 1 118 2.07e-21 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1410550 AT5G20100.1 47.312 93 33 4 4 81 3 94 1.59e-17 72.0

Find 21 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACGTTGACCGATCGATA+TGG 0.363606 tig0023670:+60250 None:intergenic
AAGGTTCATCAGGATTGGTT+TGG 0.367840 tig0023670:-60369 Msa1410550:CDS
TGGGATGATAAATTCTTGTC+TGG 0.375061 tig0023670:-60349 Msa1410550:CDS
AGGTTCATCAGGATTGGTTT+GGG 0.391343 tig0023670:-60368 Msa1410550:CDS
TTCAGGAGACAAGGTTCATC+AGG 0.416255 tig0023670:-60379 Msa1410550:CDS
AGAAATCATTAGCATCATTC+AGG 0.448473 tig0023670:-60396 Msa1410550:CDS
GAGACAAGGTTCATCAGGAT+TGG 0.459463 tig0023670:-60374 Msa1410550:CDS
AAACCACATGTTGAAACCTT+AGG 0.466270 tig0023670:+60202 None:intergenic
TTGGAGAGAAGCAGATCAGC+TGG 0.478654 tig0023670:-60280 Msa1410550:CDS
TTTGTGGAAGAAGCAGCAAC+TGG 0.482811 tig0023670:+60160 None:intergenic
GAACAAGAGAGTGCATCAAC+TGG 0.483164 tig0023670:-60307 Msa1410550:CDS
AATTGACCCACCTTCTCCTA+AGG 0.490014 tig0023670:-60218 Msa1410550:CDS
TCTTGATTTAGGATTATTTG+TGG 0.511223 tig0023670:+60144 None:intergenic
TCTCCTAAGGTTTCAACATG+TGG 0.535168 tig0023670:-60205 Msa1410550:CDS
GTTGAAACCTTAGGAGAAGG+TGG 0.589036 tig0023670:+60211 None:intergenic
CATGTTGAAACCTTAGGAGA+AGG 0.634843 tig0023670:+60208 None:intergenic
GAAGGTGGGTCAATTGATGT+TGG 0.642101 tig0023670:+60226 None:intergenic
TGGAGCAAGACCATATCGAT+CGG 0.648883 tig0023670:-60260 Msa1410550:CDS
TTGAAACCTTAGGAGAAGGT+GGG 0.649865 tig0023670:+60212 None:intergenic
TTAGCATCATTCAGGAGACA+AGG 0.658604 tig0023670:-60388 Msa1410550:CDS
GAGTGCATCAACTGGCACGT+TGG 0.684698 tig0023670:-60299 Msa1410550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTGATTTAGGATTATTTG+TGG + tig0023670:60397-60416 None:intergenic 25.0%
!! GATCTTCTTTTTCTTGATTT+AGG + tig0023670:60408-60427 None:intergenic 25.0%
AGAAATCATTAGCATCATTC+AGG - tig0023670:60142-60161 Msa1410550:CDS 30.0%
TGGGATGATAAATTCTTGTC+TGG - tig0023670:60189-60208 Msa1410550:CDS 35.0%
AAACCACATGTTGAAACCTT+AGG + tig0023670:60339-60358 None:intergenic 35.0%
! GTTTCAACATGTGGTTTTTG+TGG - tig0023670:60342-60361 Msa1410550:CDS 35.0%
!!! TGTGGTTTTTGTGGTTTGTT+TGG - tig0023670:60351-60370 Msa1410550:CDS 35.0%
TTAGCATCATTCAGGAGACA+AGG - tig0023670:60150-60169 Msa1410550:CDS 40.0%
!! AAGGTTCATCAGGATTGGTT+TGG - tig0023670:60169-60188 Msa1410550:CDS 40.0%
!! AGGTTCATCAGGATTGGTTT+GGG - tig0023670:60170-60189 Msa1410550:CDS 40.0%
TTGAAACCTTAGGAGAAGGT+GGG + tig0023670:60329-60348 None:intergenic 40.0%
CATGTTGAAACCTTAGGAGA+AGG + tig0023670:60333-60352 None:intergenic 40.0%
TCTCCTAAGGTTTCAACATG+TGG - tig0023670:60333-60352 Msa1410550:CDS 40.0%
TTCAGGAGACAAGGTTCATC+AGG - tig0023670:60159-60178 Msa1410550:CDS 45.0%
GAGACAAGGTTCATCAGGAT+TGG - tig0023670:60164-60183 Msa1410550:CDS 45.0%
! GAACAAGAGAGTGCATCAAC+TGG - tig0023670:60231-60250 Msa1410550:CDS 45.0%
TGGAGCAAGACCATATCGAT+CGG - tig0023670:60278-60297 Msa1410550:CDS 45.0%
GAAGGTGGGTCAATTGATGT+TGG + tig0023670:60315-60334 None:intergenic 45.0%
AATTGACCCACCTTCTCCTA+AGG - tig0023670:60320-60339 Msa1410550:CDS 45.0%
GTTGAAACCTTAGGAGAAGG+TGG + tig0023670:60330-60349 None:intergenic 45.0%
TTTGTGGAAGAAGCAGCAAC+TGG + tig0023670:60381-60400 None:intergenic 45.0%
TTGGAGAGAAGCAGATCAGC+TGG - tig0023670:60258-60277 Msa1410550:CDS 50.0%
GCAACGTTGACCGATCGATA+TGG + tig0023670:60291-60310 None:intergenic 50.0%
GAGTGCATCAACTGGCACGT+TGG - tig0023670:60239-60258 Msa1410550:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0023670 gene 60129 60431 60129 ID=Msa1410550;Name=Msa1410550
tig0023670 mRNA 60129 60431 60129 ID=Msa1410550-mRNA-1;Parent=Msa1410550;Name=Msa1410550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|100
tig0023670 exon 60129 60431 60129 ID=Msa1410550-mRNA-1:exon:26617;Parent=Msa1410550-mRNA-1
tig0023670 CDS 60129 60431 60129 ID=Msa1410550-mRNA-1:cds;Parent=Msa1410550-mRNA-1
Gene Sequence

>Msa1410550

ATGGCTGAGCTGCAGAAATCATTAGCATCATTCAGGAGACAAGGTTCATCAGGATTGGTTTGGGATGATAAATTCTTGTCTGGTTTGAATCAGAATCAACTTGAACAAGAGAGTGCATCAACTGGCACGTTGGAGAGAAGCAGATCAGCTGGAGCAAGACCATATCGATCGGTCAACGTTGCACCAACATCAATTGACCCACCTTCTCCTAAGGTTTCAACATGTGGTTTTTGTGGTTTGTTTGGTAAACCAGTTGCTGCTTCTTCCACAAATAATCCTAAATCAAGAAAAAGAAGATCATGA

Protein sequence

>Msa1410550

MAELQKSLASFRRQGSSGLVWDDKFLSGLNQNQLEQESASTGTLERSRSAGARPYRSVNVAPTSIDPPSPKVSTCGFCGLFGKPVAASSTNNPKSRKRRS*