AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0485710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0485710 MtrunA17_Chr3g0109721 99.500 200 1 0 1 200 1 200 5.33e-150 414
Msa0485710 MtrunA17_Chr5g0433561 93.000 200 14 0 1 200 1 200 2.27e-142 394
Msa0485710 MtrunA17_Chr8g0390561 68.912 193 59 1 8 200 7 198 2.11e-98 283
Msa0485710 MtrunA17_Chr1g0199331 65.868 167 56 1 8 173 29 195 7.81e-80 236
Msa0485710 MtrunA17_Chr4g0055651 44.103 195 100 4 12 200 10 201 4.66e-52 166
Msa0485710 MtrunA17_Chr4g0032201 42.781 187 99 2 12 193 14 197 1.15e-49 160
Msa0485710 MtrunA17_Chr4g0040781 39.806 206 103 3 12 200 15 216 1.35e-49 160
Msa0485710 MtrunA17_Chr8g0381841 47.436 156 82 0 12 167 19 174 2.19e-49 160
Msa0485710 MtrunA17_Chr6g0458081 43.750 160 90 0 12 171 8 167 3.59e-49 159
Msa0485710 MtrunA17_Chr8g0349681 39.698 199 110 1 12 200 386 584 4.67e-49 167
Msa0485710 MtrunA17_Chr8g0349681 43.210 162 92 0 12 173 177 338 6.17e-45 156
Msa0485710 MtrunA17_Chr8g0349681 43.791 153 86 0 12 164 15 167 4.84e-43 151
Msa0485710 MtrunA17_Chr2g0315551 39.806 206 103 3 12 200 15 216 7.36e-49 158
Msa0485710 MtrunA17_Chr3g0112681 41.089 202 106 3 12 200 14 215 9.90e-49 158
Msa0485710 MtrunA17_Chr2g0319951 45.679 162 88 0 12 173 15 176 1.10e-48 158
Msa0485710 MtrunA17_Chr8g0349711 43.210 162 92 0 12 173 8 169 1.22e-48 157
Msa0485710 MtrunA17_Chr2g0283131 39.320 206 104 3 12 200 15 216 1.51e-48 157
Msa0485710 MtrunA17_Chr4g0046261 39.806 206 103 3 12 200 15 216 1.52e-48 157
Msa0485710 MtrunA17_Chr2g0276941 48.366 153 79 0 12 164 17 169 1.72e-48 157
Msa0485710 MtrunA17_Chr3g0123821 46.795 156 83 0 12 167 18 173 2.28e-48 157
Msa0485710 MtrunA17_Chr3g0123971 43.478 161 91 0 12 172 11 171 2.83e-48 156
Msa0485710 MtrunA17_Chr8g0382221 43.478 161 91 0 12 172 11 171 3.43e-48 156
Msa0485710 MtrunA17_Chr6g0482721 47.500 160 84 0 12 171 14 173 8.03e-48 155
Msa0485710 MtrunA17_Chr7g0250531 40.104 192 112 1 12 200 10 201 1.67e-47 154
Msa0485710 MtrunA17_Chr1g0189361 39.423 208 107 2 12 200 16 223 3.60e-47 154
Msa0485710 MtrunA17_Chr7g0275991 42.945 163 93 0 12 174 22 184 4.54e-47 154
Msa0485710 MtrunA17_Chr5g0400131 40.341 176 105 0 12 187 11 186 6.86e-47 153
Msa0485710 MtrunA17_Chr2g0290891 43.312 157 89 0 12 168 8 164 8.41e-47 153
Msa0485710 MtrunA17_Chr8g0335471 41.026 195 107 3 12 200 10 202 1.52e-46 152
Msa0485710 MtrunA17_Chr1g0182301 38.693 199 110 4 12 199 17 214 2.84e-46 152
Msa0485710 MtrunA17_Chr4g0055081 41.615 161 94 0 12 172 11 171 5.29e-46 150
Msa0485710 MtrunA17_Chr8g0351861 46.405 153 82 0 12 164 18 170 5.52e-46 151
Msa0485710 MtrunA17_Chr7g0231131 38.424 203 110 2 12 199 17 219 6.19e-46 151
Msa0485710 MtrunA17_Chr8g0346421 41.899 179 99 1 12 185 28 206 6.33e-46 151
Msa0485710 MtrunA17_Chr1g0182321 38.693 199 110 4 12 199 17 214 6.91e-46 150
Msa0485710 MtrunA17_Chr7g0234201 40.000 205 103 4 12 200 15 215 1.08e-45 150
Msa0485710 MtrunA17_Chr1g0174281 43.827 162 91 0 12 173 17 178 3.49e-45 149
Msa0485710 MtrunA17_Chr2g0325091 43.125 160 91 0 12 171 13 172 4.62e-45 148
Msa0485710 MtrunA17_Chr1g0212521 38.889 198 111 4 12 199 17 214 1.71e-44 147
Msa0485710 MtrunA17_Chr4g0030911 38.071 197 113 3 12 199 17 213 4.41e-44 146
Msa0485710 MtrunA17_Chr7g0214241 41.875 160 93 0 12 171 17 176 5.46e-44 146
Msa0485710 MtrunA17_Chr2g0276931 40.936 171 100 1 12 182 11 180 3.65e-43 143
Msa0485710 MtrunA17_Chr7g0263071 41.104 163 95 1 12 173 17 179 1.32e-42 142
Msa0485710 MtrunA17_Chr8g0392931 46.795 156 83 0 12 167 16 171 4.29e-40 136
Msa0485710 MtrunA17_Chr2g0327911 34.184 196 121 4 12 199 17 212 1.83e-39 134
Msa0485710 MtrunA17_Chr8g0383261 36.598 194 116 4 12 198 14 207 6.17e-38 130
Msa0485710 MtrunA17_Chr8g0384311 36.923 195 115 5 12 199 15 208 8.95e-38 129
Msa0485710 MtrunA17_Chr7g0265001 36.453 203 119 4 7 200 13 214 5.47e-37 128
Msa0485710 MtrunA17_Chr1g0159481 36.041 197 117 6 12 200 19 214 1.52e-35 124
Msa0485710 MtrunA17_Chr1g0180291 36.318 201 117 5 8 199 12 210 1.79e-35 124
Msa0485710 MtrunA17_Chr1g0197331 36.041 197 117 5 12 200 17 212 2.20e-35 124
Msa0485710 MtrunA17_Chr3g0130271 36.224 196 116 6 12 199 14 208 5.22e-35 122
Msa0485710 MtrunA17_Chr6g0466301 39.394 165 93 3 12 169 10 174 9.96e-35 122
Msa0485710 MtrunA17_Chr6g0465491 39.394 165 93 3 12 169 10 174 9.96e-35 122
Msa0485710 MtrunA17_Chr4g0034871 38.788 165 94 3 12 169 10 174 3.25e-34 120
Msa0485710 MtrunA17_Chr1g0201121 39.394 165 93 3 12 169 10 174 3.67e-33 117
Msa0485710 MtrunA17_Chr2g0331691 34.595 185 114 3 1 178 1 185 1.40e-32 116
Msa0485710 MtrunA17_Chr4g0004661 35.632 174 105 3 12 178 66 239 4.79e-31 114
Msa0485710 MtrunA17_Chr3g0138121 33.526 173 109 4 12 181 15 184 4.82e-26 100
Msa0485710 MtrunA17_Chr3g0138101 33.526 173 109 4 12 181 15 184 4.82e-26 100
Msa0485710 MtrunA17_Chr3g0138111 33.526 173 109 4 12 181 15 184 4.87e-26 99.8
Msa0485710 MtrunA17_Chr2g0331841 69.492 59 18 0 78 136 1 59 8.61e-24 90.5
Msa0485710 MtrunA17_Chr8g0371501 33.939 165 95 4 12 164 8 170 3.72e-21 86.7
Msa0485710 MtrunA17_Chr6g0465471 33.333 147 91 3 40 179 20 166 6.64e-21 86.3
Msa0485710 MtrunA17_Chr6g0466161 34.307 137 83 3 40 169 20 156 8.91e-21 85.9
Msa0485710 MtrunA17_Chr5g0405791 33.333 165 96 4 12 164 8 170 1.73e-20 84.7
Msa0485710 MtrunA17_Chr3g0117931 32.335 167 95 6 12 164 10 172 2.22e-20 84.7
Msa0485710 MtrunA17_Chr4g0046211 34.426 122 77 3 12 132 8 127 4.31e-19 79.7
Msa0485710 MtrunA17_Chr4g0037021 30.909 165 100 4 12 164 8 170 9.10e-19 80.1
Msa0485710 MtrunA17_Chr6g0486041 31.138 167 97 5 12 164 8 170 9.25e-19 80.5
Msa0485710 MtrunA17_Chr2g0325101 30.303 165 101 4 12 164 8 170 1.26e-18 79.7
Msa0485710 MtrunA17_Chr6g0466431 37.113 97 52 1 12 108 10 97 2.03e-17 74.3
Msa0485710 MtrunA17_Chr6g0465611 37.113 97 52 1 12 108 10 97 2.03e-17 74.3
Msa0485710 MtrunA17_Chr5g0443631 30.588 170 108 4 9 171 103 269 1.14e-15 73.6
Msa0485710 MtrunA17_Chr2g0331831 47.692 65 33 1 136 200 2 65 1.21e-15 68.6
Msa0485710 MtrunA17_Chr4g0024431 30.539 167 106 4 12 171 93 256 2.23e-14 69.7
Msa0485710 MtrunA17_Chr1g0178291 28.144 167 110 4 13 176 431 590 4.94e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0485710 AT5G45130.1 79.500 200 41 0 1 200 1 200 9.05e-120 338
Msa0485710 AT4G19640.1 78.500 200 43 0 1 200 1 200 7.59e-119 335
Msa0485710 AT5G45130.2 81.283 187 35 0 14 200 22 208 5.67e-114 323
Msa0485710 AT4G19640.2 84.286 140 22 0 1 140 1 140 2.12e-83 244
Msa0485710 AT4G19640.3 82.394 142 25 0 1 142 1 142 3.82e-83 245
Msa0485710 AT3G54840.1 63.158 171 62 1 8 177 31 201 1.87e-78 233
Msa0485710 AT3G54840.3 63.253 166 60 1 13 177 2 167 2.49e-76 227
Msa0485710 AT3G54840.2 63.291 158 57 1 8 164 31 188 9.75e-72 216
Msa0485710 AT4G18800.1 40.201 199 109 2 12 200 15 213 1.68e-50 162
Msa0485710 AT4G39990.1 39.320 206 106 4 12 200 19 222 2.00e-50 163
Msa0485710 AT5G45750.1 39.801 201 109 2 12 200 15 215 3.48e-50 162
Msa0485710 AT1G16920.1 47.531 162 85 0 12 173 15 176 8.49e-50 161
Msa0485710 AT3G07410.1 42.365 203 103 3 12 200 14 216 1.18e-49 160
Msa0485710 AT1G07410.1 43.316 187 97 2 12 193 14 196 2.20e-49 160
Msa0485710 AT1G06400.1 40.796 201 107 2 12 200 15 215 2.30e-49 160
Msa0485710 AT5G60860.1 38.614 202 111 2 12 200 15 216 6.37e-49 159
Msa0485710 AT1G09630.1 46.914 162 86 0 12 173 14 175 9.01e-49 158
Msa0485710 AT2G44610.1 41.304 184 108 0 12 195 11 194 2.03e-48 157
Msa0485710 AT4G18430.1 38.614 202 111 2 12 200 15 216 2.15e-48 157
Msa0485710 AT4G17160.1 44.586 157 87 0 12 168 8 164 2.28e-48 157
Msa0485710 AT1G28550.1 43.827 162 91 0 12 173 15 176 3.93e-48 157
Msa0485710 AT3G15060.1 44.444 162 90 0 12 173 15 176 4.79e-48 156
Msa0485710 AT1G02130.1 41.538 195 106 3 12 200 10 202 4.80e-48 156
Msa0485710 AT2G33870.1 44.444 162 90 0 12 173 15 176 1.15e-47 155
Msa0485710 AT4G35860.1 43.125 160 91 0 12 171 8 167 1.94e-47 155
Msa0485710 AT2G22290.1 38.265 196 114 2 12 200 11 206 2.84e-47 154
Msa0485710 AT5G10260.1 42.857 161 92 0 12 172 11 171 3.42e-47 154
Msa0485710 AT2G43130.1 39.500 200 110 2 12 200 14 213 3.76e-47 154
Msa0485710 AT5G65270.1 45.752 153 83 0 12 164 19 171 6.81e-47 154
Msa0485710 AT5G47520.1 40.291 206 105 4 12 200 16 220 7.02e-47 154
Msa0485710 AT3G46830.1 40.291 206 103 4 12 200 14 216 7.45e-47 153
Msa0485710 AT3G46830.2 40.291 206 103 4 12 200 14 216 7.45e-47 153
Msa0485710 AT5G59150.1 46.405 153 82 0 12 164 14 166 2.18e-46 152
Msa0485710 AT3G12160.1 45.062 162 89 0 12 173 17 178 3.33e-46 152
Msa0485710 AT4G17170.1 42.675 157 90 0 12 168 8 164 5.78e-46 151
Msa0485710 AT5G47200.1 39.583 192 113 1 12 200 10 201 7.17e-46 150
Msa0485710 AT4G17530.1 39.691 194 110 3 12 200 10 201 7.65e-46 150
Msa0485710 AT4G39890.1 41.975 162 93 1 12 172 11 172 1.52e-45 150
Msa0485710 AT5G47960.1 42.857 168 96 0 12 179 17 184 2.65e-45 150
Msa0485710 AT5G64990.1 43.750 160 90 0 12 171 9 168 1.03e-44 147
Msa0485710 AT3G11730.1 37.113 194 115 1 12 198 10 203 2.38e-44 147
Msa0485710 AT5G59840.1 38.384 198 112 4 12 199 17 214 3.29e-44 147
Msa0485710 AT1G05810.1 38.424 203 111 3 12 200 14 216 9.04e-44 145
Msa0485710 AT1G73640.1 44.375 160 89 0 12 171 15 174 1.15e-43 146
Msa0485710 AT3G46060.2 37.374 198 114 4 12 199 17 214 2.44e-43 144
Msa0485710 AT3G46060.3 37.374 198 114 4 12 199 17 214 2.44e-43 144
Msa0485710 AT3G46060.1 37.374 198 114 4 12 199 17 214 2.44e-43 144
Msa0485710 AT1G05810.2 38.424 203 111 3 12 200 60 262 2.46e-43 146
Msa0485710 AT1G18200.1 43.750 160 90 0 12 171 15 174 5.96e-43 144
Msa0485710 AT3G53610.2 38.384 198 112 4 12 199 17 214 2.19e-42 142
Msa0485710 AT3G53610.3 38.384 198 112 4 12 199 17 214 2.19e-42 142
Msa0485710 AT3G53610.1 38.384 198 112 4 12 199 17 214 2.19e-42 142
Msa0485710 AT2G31680.1 36.765 204 114 3 12 200 14 217 2.24e-42 142
Msa0485710 AT5G64990.2 41.916 167 90 1 12 171 9 175 7.31e-42 140
Msa0485710 AT1G01200.1 41.489 188 103 3 12 194 30 215 2.39e-41 140
Msa0485710 AT5G03520.1 39.394 198 111 4 12 200 17 214 4.83e-41 139
Msa0485710 AT3G09900.1 37.500 200 114 3 12 200 17 216 2.84e-40 137
Msa0485710 AT4G35860.2 45.000 120 66 0 52 171 2 121 2.46e-36 125
Msa0485710 AT1G22740.1 40.000 165 92 3 12 169 10 174 1.40e-35 124
Msa0485710 AT1G43890.3 40.123 162 94 3 12 171 15 175 2.61e-35 124
Msa0485710 AT1G43890.1 40.123 162 94 3 12 171 15 175 2.61e-35 124
Msa0485710 AT1G43890.2 40.123 162 94 3 12 171 15 175 2.61e-35 124
Msa0485710 AT5G03530.2 36.634 202 117 6 8 200 11 210 2.98e-34 121
Msa0485710 AT5G03530.1 36.634 202 117 6 8 200 11 210 2.98e-34 121
Msa0485710 AT1G52280.1 35.795 176 106 3 12 180 10 185 5.88e-34 120
Msa0485710 AT3G18820.1 38.788 165 94 3 12 169 10 174 1.03e-33 119
Msa0485710 AT3G16100.1 36.207 174 104 3 12 178 10 183 5.68e-33 117
Msa0485710 AT2G21880.1 37.195 164 96 3 12 168 11 174 7.23e-33 117
Msa0485710 AT5G59150.2 46.903 113 60 0 52 164 3 115 1.25e-32 115
Msa0485710 AT1G49300.2 37.805 164 95 3 12 168 10 173 2.42e-32 116
Msa0485710 AT1G49300.1 37.805 164 95 3 12 168 10 173 2.42e-32 116
Msa0485710 AT5G03520.2 37.423 163 93 4 47 200 42 204 4.56e-32 115
Msa0485710 AT5G39620.1 36.145 166 99 3 12 170 7 172 9.70e-32 114
Msa0485710 AT4G09720.1 38.182 165 95 3 12 169 10 174 3.43e-31 113
Msa0485710 AT3G09910.3 34.872 195 124 3 8 200 11 204 5.98e-30 110
Msa0485710 AT3G09910.1 34.872 195 124 3 8 200 11 204 5.98e-30 110
Msa0485710 AT3G09910.2 34.872 195 124 3 8 200 11 204 5.98e-30 110
Msa0485710 AT4G09720.4 37.647 170 94 4 12 169 10 179 3.13e-29 108
Msa0485710 AT3G09910.5 38.095 168 101 3 8 173 11 177 3.06e-28 105
Msa0485710 AT3G09910.4 38.095 168 101 3 8 173 11 177 3.06e-28 105
Msa0485710 AT4G09720.3 35.795 176 95 4 12 169 10 185 5.69e-28 105
Msa0485710 AT5G55080.1 31.875 160 106 3 12 171 15 171 1.19e-26 102
Msa0485710 AT5G55190.1 34.375 160 102 3 12 171 15 171 1.27e-25 99.4
Msa0485710 AT5G20010.1 34.375 160 102 3 12 171 15 171 1.40e-25 99.0
Msa0485710 AT5G20020.1 34.375 160 102 3 12 171 15 171 1.46e-25 99.0
Msa0485710 AT2G21880.4 35.374 147 88 3 29 168 3 149 1.89e-25 97.8
Msa0485710 AT2G21880.3 35.374 147 88 3 29 168 3 149 1.89e-25 97.8
Msa0485710 AT4G09720.6 37.956 137 78 3 40 169 4 140 1.36e-23 92.8
Msa0485710 AT4G09720.5 37.956 137 78 3 40 169 4 140 1.36e-23 92.8
Msa0485710 AT4G09720.2 37.956 137 78 3 40 169 4 140 1.36e-23 92.8
Msa0485710 AT2G21880.2 32.317 164 96 4 12 168 11 166 4.63e-23 92.0
Msa0485710 AT2G44690.1 34.706 170 97 5 12 169 20 187 1.45e-21 88.2
Msa0485710 AT4G35950.1 34.545 165 94 5 12 164 8 170 1.10e-20 85.5
Msa0485710 AT3G51300.1 32.530 166 98 4 11 164 7 170 1.26e-20 85.5
Msa0485710 AT2G17800.2 33.333 165 96 4 12 164 8 170 2.37e-20 84.7
Msa0485710 AT2G17800.1 33.333 165 96 4 12 164 8 170 2.37e-20 84.7
Msa0485710 AT4G28950.1 32.121 165 96 5 12 164 8 168 3.63e-20 84.7
Msa0485710 AT3G48040.1 30.539 167 98 5 12 164 10 172 3.42e-19 82.0
Msa0485710 AT5G46025.1 45.349 86 46 1 80 164 14 99 3.63e-19 79.3
Msa0485710 AT5G62880.1 32.335 167 95 6 12 164 10 172 4.09e-19 82.0
Msa0485710 AT4G35020.1 32.727 165 97 5 12 164 8 170 5.48e-19 81.3
Msa0485710 AT4G35020.2 32.727 165 97 5 12 164 8 170 5.48e-19 81.3
Msa0485710 AT4G35020.3 32.727 165 97 5 12 164 8 170 5.48e-19 81.3
Msa0485710 AT1G75840.1 31.515 165 99 5 12 164 8 170 1.78e-18 79.7
Msa0485710 AT5G45970.1 30.909 165 100 4 12 164 8 170 1.94e-18 79.7
Msa0485710 AT1G20090.1 31.515 165 99 5 12 164 7 169 2.76e-18 79.3
Msa0485710 AT3G48040.2 33.333 129 79 4 12 137 10 134 3.82e-17 75.9
Msa0485710 AT5G62880.2 33.333 129 79 4 12 137 10 134 3.95e-17 76.3
Msa0485710 AT3G51290.2 34.884 129 71 3 48 164 644 771 5.25e-16 76.3
Msa0485710 AT4G35020.4 33.621 116 70 4 12 124 8 119 4.84e-14 67.0
Msa0485710 AT4G35020.5 33.621 116 70 4 12 124 8 119 4.84e-14 67.0
Msa0485710 AT5G54840.1 30.952 168 104 6 12 171 103 266 1.22e-13 68.2
Msa0485710 AT3G21700.3 28.070 171 112 5 9 171 105 272 4.24e-12 63.9

Find 65 sgRNAs with CRISPR-Local

Find 317 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCCCTTGGATGCATATT+TGG 0.119558 3_4:-3450163 Msa0485710:three_prime_UTR
CAAAACAATACAAATTAAAA+AGG 0.201113 3_4:+3450287 None:intergenic
TCAGGTTCTTCTTGGTGATA+TGG 0.214614 3_4:-3454799 Msa0485710:intron
GGGTCAATTTCTCGAATTTC+AGG 0.243303 3_4:-3454739 Msa0485710:intron
AAGATTTAAGCTTTGAAGTT+AGG 0.257925 3_4:+3454991 None:intergenic
CAACAATCTCAATGCTAAAT+TGG 0.259093 3_4:-3454895 Msa0485710:intron
CAACGGTTAAATTTGAGATT+TGG 0.280373 3_4:-3453401 Msa0485710:CDS
GCAAGAGCCATGACCATGTT+TGG 0.310928 3_4:+3451920 None:intergenic
AACGGTTAAATTTGAGATTT+GGG 0.317804 3_4:-3453400 Msa0485710:CDS
CTTGGGTTATCACTGTTATA+TGG 0.319977 3_4:-3450239 Msa0485710:three_prime_UTR
ATTAACATTGGCTGCAGATT+TGG 0.324617 3_4:+3451288 None:intergenic
AGTGTTTAAGCTGTATTTAC+CGG 0.350418 3_4:-3450315 Msa0485710:three_prime_UTR
TATCCCTTGGATGCATATTT+GGG 0.359069 3_4:-3450162 Msa0485710:three_prime_UTR
CGTGTATATGCCGAGGAAAA+TGG 0.363462 3_4:-3451334 Msa0485710:CDS
AACATGGTCATGGCTCTTGC+TGG 0.370648 3_4:-3451917 Msa0485710:CDS
GTGGTGCAGGAATCAACAAT+AGG 0.376165 3_4:-3453462 Msa0485710:intron
CGTAGCCATGAGAAGAGAAT+TGG 0.383455 3_4:+3454926 None:intergenic
CAGGTTCTTCTTGGTGATAT+GGG 0.397120 3_4:-3454798 Msa0485710:CDS
AGATTTAAGCTTTGAAGTTA+GGG 0.398448 3_4:+3454992 None:intergenic
ATAGACCTGCCGAAGGATCT+AGG 0.399113 3_4:-3450358 Msa0485710:CDS
GAATATCTAAATTATGTTCC+CGG 0.401727 3_4:+3450068 None:intergenic
CAAGAGCCATGACCATGTTT+GGG 0.423378 3_4:+3451921 None:intergenic
TTTATGTACTGCACAGAACT+TGG 0.427414 3_4:-3450257 Msa0485710:three_prime_UTR
TGGTGCAGGAATCAACAATA+GGG 0.434766 3_4:-3453461 Msa0485710:intron
CTTCTTGGTGATATGGGTGC+TGG 0.450654 3_4:-3454792 Msa0485710:CDS
GATCGAAAGTGAAGAGAGAA+AGG 0.471446 3_4:+3454952 None:intergenic
CGATGCAGCCCTAGATCCTT+CGG 0.472784 3_4:+3450349 None:intergenic
ATTTGCCACACAGAACTTGT+TGG 0.477154 3_4:-3450193 Msa0485710:three_prime_UTR
CAGCCAGCTCAAAACCCAGC+AGG 0.480898 3_4:-3450395 Msa0485710:CDS
TCAACAAGAACCATCCCTGC+TGG 0.485332 3_4:+3450380 None:intergenic
ATAAAATACATCATTAACAT+TGG 0.495428 3_4:+3451276 None:intergenic
TGCTGGCAACAAATCTGACT+TGG 0.506651 3_4:-3451900 Msa0485710:CDS
AGCTCAAAACCCAGCAGGGA+TGG 0.507722 3_4:-3450390 Msa0485710:CDS
AATATCTAAATTATGTTCCC+GGG 0.515074 3_4:+3450069 None:intergenic
CTATAGTCTAAAATATCTGT+TGG 0.524888 3_4:-3450125 Msa0485710:three_prime_UTR
AGCTGCGCCTCTGTAATACA+TGG 0.526016 3_4:+3453341 None:intergenic
TAGACTATAGATTTATAGCA+AGG 0.538228 3_4:+3450138 None:intergenic
TTTGAGATTTGGGACACAGC+AGG 0.542819 3_4:-3453390 Msa0485710:CDS
AAATTGCATTCATATACTGT+TGG 0.548433 3_4:+3450212 None:intergenic
TAGACCTGCCGAAGGATCTA+GGG 0.549720 3_4:-3450357 Msa0485710:CDS
TCGATCCAATTCTCTTCTCA+TGG 0.551685 3_4:-3454931 Msa0485710:exon
TTATGTACTGCACAGAACTT+GGG 0.560984 3_4:-3450256 Msa0485710:three_prime_UTR
CAACAAGAACCATCCCTGCT+GGG 0.567898 3_4:+3450381 None:intergenic
AGGACCCAAATATGCATCCA+AGG 0.569874 3_4:+3450158 None:intergenic
CCTTCTCACGAGCTAAGAAG+TGG 0.589371 3_4:-3452653 Msa0485710:CDS
ACAAGAGAGATACCATAGCT+TGG 0.597101 3_4:-3453367 Msa0485710:CDS
GCAGCCCTAGATCCTTCGGC+AGG 0.598606 3_4:+3450353 None:intergenic
ATTGGCTGCAGATTTGGCAG+AGG 0.603013 3_4:+3451294 None:intergenic
AATACATGGGAGCCAAGCTA+TGG 0.603780 3_4:+3453355 None:intergenic
GCTTGCAGGCAACCCAAACA+TGG 0.608135 3_4:-3451933 Msa0485710:intron
GTCCAAGAGCTTCAAAAGCA+AGG 0.610196 3_4:-3452630 Msa0485710:intron
GGACCCAAATATGCATCCAA+GGG 0.612652 3_4:+3450159 None:intergenic
AACAGCTAAAAGATTACCAA+GGG 0.615040 3_4:-3450420 Msa0485710:intron
CCACTTCTTAGCTCGTGAGA+AGG 0.618362 3_4:+3452653 None:intergenic
AGCCAGCTCAAAACCCAGCA+GGG 0.622894 3_4:-3450394 Msa0485710:CDS
AGGCAACCCAAACATGGTCA+TGG 0.632739 3_4:-3451927 Msa0485710:CDS
AAACAGCTAAAAGATTACCA+AGG 0.641621 3_4:-3450421 Msa0485710:intron
CTTGTTGATAGACCTGCCGA+AGG 0.644031 3_4:-3450365 Msa0485710:CDS
GGAAGCTCGTGTATATGCCG+AGG 0.658380 3_4:-3451341 Msa0485710:CDS
GCTGCGCCTCTGTAATACAT+GGG 0.659907 3_4:+3453342 None:intergenic
ATTAGCAGTAAATGACGCAA+CGG 0.668502 3_4:-3453418 Msa0485710:CDS
GGTGCAGGAATCAACAATAG+GGG 0.672375 3_4:-3453460 Msa0485710:intron
CTTCTCACGAGCTAAGAAGT+GGG 0.674595 3_4:-3452652 Msa0485710:CDS
TTGAACCAACAAGTTCTGTG+TGG 0.689121 3_4:+3450188 None:intergenic
TTGGCTCCCATGTATTACAG+AGG 0.724540 3_4:-3453348 Msa0485710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAATACAAATTAAAAA+GGG + chr3_4:3454942-3454961 None:intergenic 10.0%
!! ATCTATAATATTTAACATGA+TGG - chr3_4:3452054-3452073 Msa0485710:intron 15.0%
!!! TAACATATCAATTTTAAAAC+AGG + chr3_4:3452110-3452129 None:intergenic 15.0%
!! AATAACTTATTAATCATTTG+AGG - chr3_4:3452826-3452845 Msa0485710:intron 15.0%
!!! GATTATTGATTATAGTATAT+TGG + chr3_4:3453817-3453836 None:intergenic 15.0%
!! ATAAAATACATCATTAACAT+TGG + chr3_4:3453954-3453973 None:intergenic 15.0%
!!! AATGATGTATTTTATGAAAT+AGG - chr3_4:3453959-3453978 Msa0485710:intron 15.0%
!! CAAAACAATACAAATTAAAA+AGG + chr3_4:3454943-3454962 None:intergenic 15.0%
!! ATCAGAATTAAAAAAGATGA+AGG + chr3_4:3450383-3450402 None:intergenic 20.0%
!!! TCATCATTTTTGATTTGATT+AGG - chr3_4:3450517-3450536 Msa0485710:intron 20.0%
!!! AAGATTTTTCAATTAGAAGT+TGG - chr3_4:3450619-3450638 Msa0485710:intron 20.0%
!!! AGATTTTTCAATTAGAAGTT+GGG - chr3_4:3450620-3450639 Msa0485710:intron 20.0%
!!! ACTAATTTTCAAAGTTATTG+AGG - chr3_4:3450690-3450709 Msa0485710:intron 20.0%
!! TACAATAAATAATTTCGTGA+TGG - chr3_4:3451130-3451149 Msa0485710:intron 20.0%
!! AATTGAATGTAAATTTGTGT+AGG + chr3_4:3451458-3451477 None:intergenic 20.0%
!!! GTAAGTATAAGTTAGAATTT+TGG - chr3_4:3451552-3451571 Msa0485710:intron 20.0%
!!! ATTTTGTTCACAAGTTTATA+TGG - chr3_4:3451736-3451755 Msa0485710:intron 20.0%
!! TCAAATAATAAGTATCATTC+TGG + chr3_4:3452250-3452269 None:intergenic 20.0%
!!! AATTTTGTACCAATTATAGT+TGG + chr3_4:3452359-3452378 None:intergenic 20.0%
!! ACTATAATTGGTACAAAATT+TGG - chr3_4:3452359-3452378 Msa0485710:intron 20.0%
!! TAGAATTGATATATTGCAAA+AGG - chr3_4:3452677-3452696 Msa0485710:intron 20.0%
!!! TGTTTTACTAGATTCAATTT+TGG - chr3_4:3452803-3452822 Msa0485710:intron 20.0%
!! ATTCTTAATCAACATACAAT+AGG - chr3_4:3453106-3453125 Msa0485710:intron 20.0%
!! CAGATTAAAGCATATATAAT+AGG + chr3_4:3453995-3454014 None:intergenic 20.0%
!!! AAATGGTCTTATTTTATTCT+TGG - chr3_4:3454019-3454038 Msa0485710:intron 20.0%
!!! CTAAACTTTCCTTTTTATAA+GGG + chr3_4:3454051-3454070 None:intergenic 20.0%
!! CTTATAAAAAGGAAAGTTTA+GGG - chr3_4:3454050-3454069 Msa0485710:intron 20.0%
!! TTATAAAAAGGAAAGTTTAG+GGG - chr3_4:3454051-3454070 Msa0485710:intron 20.0%
!! TTTATCAAATCTTCTCTAAT+TGG - chr3_4:3454196-3454215 Msa0485710:intron 20.0%
!! CATGAAATTTAACAGAAATT+CGG + chr3_4:3454668-3454687 None:intergenic 20.0%
!! ATCATAAAATGTAGATCAAT+TGG + chr3_4:3454705-3454724 None:intergenic 20.0%
!!! TTAATTTGTATTGTTTTGAG+TGG - chr3_4:3454945-3454964 Msa0485710:five_prime_UTR 20.0%
!!! AGATTTAAGCTTTGAAGTTA+GGG + chr3_4:3450238-3450257 None:intergenic 25.0%
!!! AAGATTTAAGCTTTGAAGTT+AGG + chr3_4:3450239-3450258 None:intergenic 25.0%
! GATTAAAAAACGAAGTTAGA+GGG + chr3_4:3450410-3450429 None:intergenic 25.0%
! TGATTAAAAAACGAAGTTAG+AGG + chr3_4:3450411-3450430 None:intergenic 25.0%
!!! TCTAACTTCGTTTTTTAATC+AGG - chr3_4:3450410-3450429 Msa0485710:CDS 25.0%
!!! TTTTTTAATCAGGTTCTTCT+TGG - chr3_4:3450420-3450439 Msa0485710:intron 25.0%
!!! TCTTGTTTTAAGATTTGTCA+AGG - chr3_4:3450467-3450486 Msa0485710:intron 25.0%
!!! CTTGTTTTAAGATTTGTCAA+GGG - chr3_4:3450468-3450487 Msa0485710:intron 25.0%
! TACAATAAATAACTTCGTGA+TGG - chr3_4:3450946-3450965 Msa0485710:intron 25.0%
! AAATAATTTCGTGATGGTTA+TGG - chr3_4:3451136-3451155 Msa0485710:intron 25.0%
! CCACTAATAATGTAAGAAAA+TGG + chr3_4:3451317-3451336 None:intergenic 25.0%
!! CCATTTTCTTACATTATTAG+TGG - chr3_4:3451314-3451333 Msa0485710:CDS 25.0%
!!! AGCTTTAAATAATTGTAGTC+TGG - chr3_4:3451575-3451594 Msa0485710:intron 25.0%
! TTTAAATAATTGTAGTCTGG+TGG - chr3_4:3451578-3451597 Msa0485710:intron 25.0%
! TTAAATAATTGTAGTCTGGT+GGG - chr3_4:3451579-3451598 Msa0485710:intron 25.0%
! ATTATGTTAATGATGCTTCT+GGG - chr3_4:3451649-3451668 Msa0485710:intron 25.0%
!! AACGGTTAAATTTGAGATTT+GGG - chr3_4:3451827-3451846 Msa0485710:intron 25.0%
! CAAAATTACAAGTCAAAAAC+TGG + chr3_4:3452002-3452021 None:intergenic 25.0%
! TGCGAAGTATAACTTAAAAA+TGG - chr3_4:3452169-3452188 Msa0485710:intron 25.0%
! TTCATACTTATTAAACTTCC+AGG + chr3_4:3452218-3452237 None:intergenic 25.0%
! ATATTATCAGCTCTTTATCA+GGG - chr3_4:3452274-3452293 Msa0485710:intron 25.0%
!! AATTGTAATGAGGATTTTTC+TGG + chr3_4:3452314-3452333 None:intergenic 25.0%
! TATTCGTCTAATTTCTCAAT+AGG - chr3_4:3452513-3452532 Msa0485710:intron 25.0%
! ATTCGTCTAATTTCTCAATA+GGG - chr3_4:3452514-3452533 Msa0485710:intron 25.0%
!!! AAAGCAAGGTATTAACTTTT+TGG - chr3_4:3452611-3452630 Msa0485710:intron 25.0%
! AAACATAAAAGATAACACGA+GGG + chr3_4:3452788-3452807 None:intergenic 25.0%
! AAAACATAAAAGATAACACG+AGG + chr3_4:3452789-3452808 None:intergenic 25.0%
!! TGTTTTATAATAGAACAGCA+TGG - chr3_4:3452863-3452882 Msa0485710:intron 25.0%
! CATGGTATAAAAAGCAATAT+TGG + chr3_4:3453396-3453415 None:intergenic 25.0%
! GTCAAAATTGAAGTTTATGA+TGG + chr3_4:3453745-3453764 None:intergenic 25.0%
!!! TAATCAATAATCACTTTTCC+TGG - chr3_4:3453825-3453844 Msa0485710:intron 25.0%
!! CCTAAACTTTCCTTTTTATA+AGG + chr3_4:3454052-3454071 None:intergenic 25.0%
! CCTTATAAAAAGGAAAGTTT+AGG - chr3_4:3454049-3454068 Msa0485710:intron 25.0%
! AGACTTTATGAATTATGAGA+AGG + chr3_4:3454257-3454276 None:intergenic 25.0%
! TTCATAAAGTCTTCTCTAAT+TGG - chr3_4:3454265-3454284 Msa0485710:intron 25.0%
!! CAAAAAGGGTACATATAATA+TGG + chr3_4:3454359-3454378 None:intergenic 25.0%
!! CATATTATATGTACCCTTTT+TGG - chr3_4:3454357-3454376 Msa0485710:intron 25.0%
! AACATTAAAGTGACCAAAAA+GGG + chr3_4:3454373-3454392 None:intergenic 25.0%
! AAAGTGTAATAAGGACAAAA+GGG + chr3_4:3454408-3454427 None:intergenic 25.0%
! TAAAGTGTAATAAGGACAAA+AGG + chr3_4:3454409-3454428 None:intergenic 25.0%
! TGTTGCAATAAAGTGTAATA+AGG + chr3_4:3454417-3454436 None:intergenic 25.0%
! CCTATGAAAAAACAGAAATT+AGG + chr3_4:3454530-3454549 None:intergenic 25.0%
!!! CCTAATTTCTGTTTTTTCAT+AGG - chr3_4:3454527-3454546 Msa0485710:intron 25.0%
! TAATCAAAGTTAGCAGTTTA+TGG + chr3_4:3454759-3454778 None:intergenic 25.0%
! CAATACAAATTAAAAAGGGA+AGG + chr3_4:3454938-3454957 None:intergenic 25.0%
! AAATTGCATTCATATACTGT+TGG + chr3_4:3455018-3455037 None:intergenic 25.0%
! TAGACTATAGATTTATAGCA+AGG + chr3_4:3455092-3455111 None:intergenic 25.0%
!! CTATAGTCTAAAATATCTGT+TGG - chr3_4:3455102-3455121 Msa0485710:five_prime_UTR 25.0%
!! TAATAAAATGAGATTTTGCC+CGG - chr3_4:3455140-3455159 Msa0485710:five_prime_UTR 25.0%
!! ATTCTATTGCTTTTCGCTTA+CGG + chr3_4:3450151-3450170 None:intergenic 30.0%
!! AGCTTAAATCTTTCGCTTTT+TGG - chr3_4:3450247-3450266 Msa0485710:three_prime_UTR 30.0%
!!! GCTTAAATCTTTCGCTTTTT+GGG - chr3_4:3450248-3450267 Msa0485710:three_prime_UTR 30.0%
CAACAATCTCAATGCTAAAT+TGG - chr3_4:3450332-3450351 Msa0485710:exon 30.0%
! AGGAAGTTGTTTAAGTTCAA+TGG - chr3_4:3450537-3450556 Msa0485710:intron 30.0%
! GGAAGTTGTTTAAGTTCAAT+GGG - chr3_4:3450538-3450557 Msa0485710:intron 30.0%
AAATAACTTCGTGATGGTTA+TGG - chr3_4:3450952-3450971 Msa0485710:intron 30.0%
TAGCACTTACACTTTGAATA+AGG - chr3_4:3451065-3451084 Msa0485710:intron 30.0%
TAAATAATTGTAGTCTGGTG+GGG - chr3_4:3451580-3451599 Msa0485710:intron 30.0%
! TTTCTTCTTTCTTTTCTCTG+AGG - chr3_4:3451614-3451633 Msa0485710:intron 30.0%
GATTATGTTAATGATGCTTC+TGG - chr3_4:3451648-3451667 Msa0485710:intron 30.0%
! CAAGTTTATATGGTCTTTTG+TGG - chr3_4:3451746-3451765 Msa0485710:intron 30.0%
! CAACGGTTAAATTTGAGATT+TGG - chr3_4:3451826-3451845 Msa0485710:intron 30.0%
! TTTTTGAATACCTACTTCGT+TGG - chr3_4:3451966-3451985 Msa0485710:intron 30.0%
! TTTTGAATACCTACTTCGTT+GGG - chr3_4:3451967-3451986 Msa0485710:intron 30.0%
AAAAATGTTCTGATTCTGAC+TGG - chr3_4:3452129-3452148 Msa0485710:intron 30.0%
AAAATGTTCTGATTCTGACT+GGG - chr3_4:3452130-3452149 Msa0485710:intron 30.0%
CATATTATCAGCTCTTTATC+AGG - chr3_4:3452273-3452292 Msa0485710:intron 30.0%
TCATTACAATTGTATGCACA+GGG - chr3_4:3452323-3452342 Msa0485710:intron 30.0%
!!! CATCAATTTTTTCCTAAACG+TGG - chr3_4:3452412-3452431 Msa0485710:intron 30.0%
TACTTCTATCTTCTATTCAC+AGG - chr3_4:3452548-3452567 Msa0485710:intron 30.0%
!!! AGGTATTAACTTTTTGGTTC+AGG - chr3_4:3452617-3452636 Msa0485710:intron 30.0%
TAAAACCATTTCCAAACTTC+TGG - chr3_4:3452715-3452734 Msa0485710:intron 30.0%
ATGGAAAATGCTTTACTAGA+AGG + chr3_4:3452747-3452766 None:intergenic 30.0%
TCAAAAGTTAAATGTGTCTC+TGG + chr3_4:3452988-3453007 None:intergenic 30.0%
TTACTATACATCTTGCTAGA+AGG - chr3_4:3453062-3453081 Msa0485710:intron 30.0%
TTCCTTTCTCTAGTAATACT+TGG - chr3_4:3453203-3453222 Msa0485710:intron 30.0%
AACCAAGTATTACTAGAGAA+AGG + chr3_4:3453208-3453227 None:intergenic 30.0%
TTCTCTAGTAATACTTGGTT+TGG - chr3_4:3453208-3453227 Msa0485710:intron 30.0%
GAGAATATTGACACTTACTT+CGG + chr3_4:3453374-3453393 None:intergenic 30.0%
! TGTTATTGATTGCTTAGGAT+AGG + chr3_4:3453476-3453495 None:intergenic 30.0%
!! TGCTTTGTTATTGATTGCTT+AGG + chr3_4:3453481-3453500 None:intergenic 30.0%
! CAATCAATAACAAAGCATCA+GGG - chr3_4:3453484-3453503 Msa0485710:intron 30.0%
! AATCAATAACAAAGCATCAG+GGG - chr3_4:3453485-3453504 Msa0485710:intron 30.0%
TTAGCAAAGAAGTTAATCAC+AGG - chr3_4:3453529-3453548 Msa0485710:intron 30.0%
!!! TCAATTGCTTTTGAAGTGTT+TGG - chr3_4:3453598-3453617 Msa0485710:intron 30.0%
! AGCATGATTGGTAATTTCAT+AGG - chr3_4:3453783-3453802 Msa0485710:intron 30.0%
TTTCGATTCATTGATATTGC+AGG - chr3_4:3453865-3453884 Msa0485710:intron 30.0%
! GAAAAACAAACCATTTTCCT+CGG + chr3_4:3453906-3453925 None:intergenic 30.0%
!!! GGAAAATGGTTTGTTTTTCA+TGG - chr3_4:3453907-3453926 Msa0485710:intron 30.0%
ATGCTTTAATCTGCAGAAAA+TGG - chr3_4:3454002-3454021 Msa0485710:intron 30.0%
TGGTTGAATCCCTTATAAAA+AGG - chr3_4:3454039-3454058 Msa0485710:intron 30.0%
TGAATATGATAGCAATGTCT+TGG + chr3_4:3454151-3454170 None:intergenic 30.0%
CAACATTAAAGTGACCAAAA+AGG + chr3_4:3454374-3454393 None:intergenic 30.0%
ACACTTTATTGCAACATTCA+TGG - chr3_4:3454421-3454440 Msa0485710:intron 30.0%
CACTTTATTGCAACATTCAT+GGG - chr3_4:3454422-3454441 Msa0485710:intron 30.0%
TAGGCAAAATGTTAGTAAGT+CGG - chr3_4:3454546-3454565 Msa0485710:intron 30.0%
TACACAAGCAATTGTAAGTT+AGG + chr3_4:3454787-3454806 None:intergenic 30.0%
AAACAGCTAAAAGATTACCA+AGG - chr3_4:3454806-3454825 Msa0485710:intron 30.0%
AACAGCTAAAAGATTACCAA+GGG - chr3_4:3454807-3454826 Msa0485710:intron 30.0%
AGTGTTTAAGCTGTATTTAC+CGG - chr3_4:3454912-3454931 Msa0485710:CDS 30.0%
! AATAAAATGAGATTTTGCCC+GGG - chr3_4:3455141-3455160 Msa0485710:five_prime_UTR 30.0%
ACATTACAGTGACAACATGT+CGG + chr3_4:3450768-3450787 None:intergenic 35.0%
ATGTTGTCACTGTAATGTGA+TGG - chr3_4:3450770-3450789 Msa0485710:intron 35.0%
TCACTGTAATGTGATGGTTA+CGG - chr3_4:3450776-3450795 Msa0485710:intron 35.0%
GTCATGTATTACCTATGCTT+TGG - chr3_4:3450811-3450830 Msa0485710:intron 35.0%
AAGACTTGAATCCAAAGCAT+AGG + chr3_4:3450825-3450844 None:intergenic 35.0%
TAGCCCTTACACTTTGATTA+AGG - chr3_4:3450882-3450901 Msa0485710:intron 35.0%
CCTCCTTAATCAAAGTGTAA+GGG + chr3_4:3450888-3450907 None:intergenic 35.0%
ACCTCCTTAATCAAAGTGTA+AGG + chr3_4:3450889-3450908 None:intergenic 35.0%
AAGACTTGAATCCGAAGTAT+CGG + chr3_4:3451008-3451027 None:intergenic 35.0%
TAATTTCGTGATGGTTATGG+AGG - chr3_4:3451139-3451158 Msa0485710:intron 35.0%
!! TACTAGTAAGTTCTGAAGCA+CGG - chr3_4:3451371-3451390 Msa0485710:intron 35.0%
AAATAATTGTAGTCTGGTGG+GGG - chr3_4:3451581-3451600 Msa0485710:intron 35.0%
!!! TTTTGAGCCCTGTTATTTTG+TGG - chr3_4:3451678-3451697 Msa0485710:intron 35.0%
! GGTGATGTGCTTTAAATGTA+TGG - chr3_4:3451703-3451722 Msa0485710:intron 35.0%
ATTAGCAGTAAATGACGCAA+CGG - chr3_4:3451809-3451828 Msa0485710:intron 35.0%
! GATGGTTAGTTAACAGACAT+AGG - chr3_4:3452072-3452091 Msa0485710:intron 35.0%
CTGTGCATACAATTGTAATG+AGG + chr3_4:3452324-3452343 None:intergenic 35.0%
CTCATTACAATTGTATGCAC+AGG - chr3_4:3452322-3452341 Msa0485710:intron 35.0%
CCAATTATAGTTGGAACAAG+TGG + chr3_4:3452350-3452369 None:intergenic 35.0%
CCACTTGTTCCAACTATAAT+TGG - chr3_4:3452347-3452366 Msa0485710:intron 35.0%
GAAGAGATCGAATATGCTAA+CGG - chr3_4:3452434-3452453 Msa0485710:intron 35.0%
AAGAGATCGAATATGCTAAC+GGG - chr3_4:3452435-3452454 Msa0485710:intron 35.0%
! GGATTCTTTCTTTTGTCTTG+TGG - chr3_4:3452456-3452475 Msa0485710:intron 35.0%
! TCTTTCTTTTGTCTTGTGGT+TGG - chr3_4:3452460-3452479 Msa0485710:intron 35.0%
! CTTTCTTTTGTCTTGTGGTT+GGG - chr3_4:3452461-3452480 Msa0485710:intron 35.0%
! TGCATGTAAGTATTGTGAAC+AGG - chr3_4:3452933-3452952 Msa0485710:intron 35.0%
! GCATGTAAGTATTGTGAACA+GGG - chr3_4:3452934-3452953 Msa0485710:intron 35.0%
CAATCAAACATTGTCTGCTT+TGG - chr3_4:3453177-3453196 Msa0485710:intron 35.0%
! AATTTCATGTTGTTGCTTGC+AGG - chr3_4:3453280-3453299 Msa0485710:intron 35.0%
GCACATAACACTTACTAACA+TGG + chr3_4:3453414-3453433 None:intergenic 35.0%
! GCAATCAATAACAAAGCATC+AGG - chr3_4:3453483-3453502 Msa0485710:intron 35.0%
CTCCATCAGATTTCACTAAT+TGG - chr3_4:3453567-3453586 Msa0485710:intron 35.0%
GACCAATTAGTGAAATCTGA+TGG + chr3_4:3453572-3453591 None:intergenic 35.0%
!!! TTGCTTTTGAAGTGTTTGGA+AGG - chr3_4:3453602-3453621 Msa0485710:intron 35.0%
!!! TGCTTTTGAAGTGTTTGGAA+GGG - chr3_4:3453603-3453622 Msa0485710:intron 35.0%
ATTACCAATCATGCTGCAAT+GGG + chr3_4:3453778-3453797 None:intergenic 35.0%
AATTACCAATCATGCTGCAA+TGG + chr3_4:3453779-3453798 None:intergenic 35.0%
ATCGAAAGTGATAAACCAGT+AGG + chr3_4:3453852-3453871 None:intergenic 35.0%
! ATTAACATTGGCTGCAGATT+TGG + chr3_4:3453942-3453961 None:intergenic 35.0%
AATATTATTAGCCCGAAGAG+TGG - chr3_4:3454074-3454093 Msa0485710:intron 35.0%
ATATTATTAGCCCGAAGAGT+GGG - chr3_4:3454075-3454094 Msa0485710:intron 35.0%
CTCTAATTGGTTATAAGCCA+TGG - chr3_4:3454209-3454228 Msa0485710:intron 35.0%
AGTCTTCTCTAATTGGCTAT+TGG - chr3_4:3454272-3454291 Msa0485710:intron 35.0%
AGGACAAAAGGGAAAATTTC+TGG + chr3_4:3454397-3454416 None:intergenic 35.0%
GATACGCCAATAACATAAAG+CGG + chr3_4:3454476-3454495 None:intergenic 35.0%
GAACCTCCTTAAAACTCATT+TGG - chr3_4:3454600-3454619 Msa0485710:intron 35.0%
!!! ACACCAAATGAGTTTTAAGG+AGG + chr3_4:3454606-3454625 None:intergenic 35.0%
! GAGACACCAAATGAGTTTTA+AGG + chr3_4:3454609-3454628 None:intergenic 35.0%
ACAAATTAAAAAGGGAAGGC+CGG + chr3_4:3454934-3454953 None:intergenic 35.0%
TTTATGTACTGCACAGAACT+TGG - chr3_4:3454970-3454989 Msa0485710:five_prime_UTR 35.0%
TTATGTACTGCACAGAACTT+GGG - chr3_4:3454971-3454990 Msa0485710:five_prime_UTR 35.0%
CTTGGGTTATCACTGTTATA+TGG - chr3_4:3454988-3455007 Msa0485710:five_prime_UTR 35.0%
! GTTGGTTCAAAAATATCCCT+TGG - chr3_4:3455052-3455071 Msa0485710:five_prime_UTR 35.0%
ATATCCCTTGGATGCATATT+TGG - chr3_4:3455064-3455083 Msa0485710:five_prime_UTR 35.0%
TATCCCTTGGATGCATATTT+GGG - chr3_4:3455065-3455084 Msa0485710:five_prime_UTR 35.0%
GATCGAAAGTGAAGAGAGAA+AGG + chr3_4:3450278-3450297 None:intergenic 40.0%
TCGATCCAATTCTCTTCTCA+TGG - chr3_4:3450296-3450315 Msa0485710:three_prime_UTR 40.0%
ATGAAGGAAGAGAAGAACAG+AGG + chr3_4:3450367-3450386 None:intergenic 40.0%
! TCAGGTTCTTCTTGGTGATA+TGG - chr3_4:3450428-3450447 Msa0485710:intron 40.0%
! CAGGTTCTTCTTGGTGATAT+GGG - chr3_4:3450429-3450448 Msa0485710:intron 40.0%
GGGTCAATTTCTCGAATTTC+AGG - chr3_4:3450488-3450507 Msa0485710:intron 40.0%
TCTTGTCTGATGTTCCACAA+TGG - chr3_4:3450841-3450860 Msa0485710:intron 40.0%
CCCTTACACTTTGATTAAGG+AGG - chr3_4:3450885-3450904 Msa0485710:intron 40.0%
TACCACAGTAATGACACATG+CGG + chr3_4:3450921-3450940 None:intergenic 40.0%
TAACTTCGTGATGGTTATGG+AGG - chr3_4:3450955-3450974 Msa0485710:intron 40.0%
CTAACTACTACCTCCATTGT+GGG + chr3_4:3450857-3450876 None:intergenic 40.0%
TAGTGACTAGTGACACATGT+CGG + chr3_4:3451207-3451226 None:intergenic 40.0%
TGTCAGTGTCAGTGTTTCAT+AGG - chr3_4:3451337-3451356 Msa0485710:CDS 40.0%
CAGGAAACACCTAATCTAAG+AGG + chr3_4:3451400-3451419 None:intergenic 40.0%
TATCCATGTCAGTGTCTTGT+CGG - chr3_4:3451512-3451531 Msa0485710:intron 40.0%
TCACCAGCCACAAAATAACA+GGG + chr3_4:3451688-3451707 None:intergenic 40.0%
ATCACCAGCCACAAAATAAC+AGG + chr3_4:3451689-3451708 None:intergenic 40.0%
! TATATGGTCTTTTGTGGTGC+AGG - chr3_4:3451752-3451771 Msa0485710:intron 40.0%
TGGTGCAGGAATCAACAATA+GGG - chr3_4:3451766-3451785 Msa0485710:intron 40.0%
ACAAGAGAGATACCATAGCT+TGG - chr3_4:3451860-3451879 Msa0485710:intron 40.0%
ATTCGATCTCTTCCACGTTT+AGG + chr3_4:3452427-3452446 None:intergenic 40.0%
!!! TACCTTGCTTTTGAAGCTCT+TGG + chr3_4:3452602-3452621 None:intergenic 40.0%
ACCATTTCCAAACTTCTGGA+TGG - chr3_4:3452719-3452738 Msa0485710:intron 40.0%
ACCATCCAGAAGTTTGGAAA+TGG + chr3_4:3452723-3452742 None:intergenic 40.0%
GACATTGACATTGGCAGATA+TGG + chr3_4:3452766-3452785 None:intergenic 40.0%
TATTATTAGCCCGAAGAGTG+GGG - chr3_4:3454076-3454095 Msa0485710:intron 40.0%
CAATGTCTTGGAGTGTAAAG+TGG + chr3_4:3454139-3454158 None:intergenic 40.0%
GAAGACAAGTTTCAGGAACA+AGG + chr3_4:3454333-3454352 None:intergenic 40.0%
! ATGGACTGAAGACAAGTTTC+AGG + chr3_4:3454340-3454359 None:intergenic 40.0%
! ATTTTCCTTCTGTTCCTAGC+AGG - chr3_4:3454447-3454466 Msa0485710:intron 40.0%
AGGCTTCCGCTTTATGTTAT+TGG - chr3_4:3454467-3454486 Msa0485710:intron 40.0%
GGAATATGTCCCACATATGT+AGG + chr3_4:3454647-3454666 None:intergenic 40.0%
ATTTGCCACACAGAACTTGT+TGG - chr3_4:3455034-3455053 Msa0485710:five_prime_UTR 40.0%
TTGAACCAACAAGTTCTGTG+TGG + chr3_4:3455042-3455061 None:intergenic 40.0%
CGTAGCCATGAGAAGAGAAT+TGG + chr3_4:3450304-3450323 None:intergenic 45.0%
CTGTAATGTGATGGTTACGG+AGG - chr3_4:3450779-3450798 Msa0485710:intron 45.0%
TGTCTGATGTTCCACAATGG+AGG - chr3_4:3450844-3450863 Msa0485710:intron 45.0%
GCTAACTACTACCTCCATTG+TGG + chr3_4:3450858-3450877 None:intergenic 45.0%
TATCGGCAATACATGGCTGA+TGG + chr3_4:3450991-3451010 None:intergenic 45.0%
GCCATGTATTGCCGATACTT+CGG - chr3_4:3450994-3451013 Msa0485710:intron 45.0%
!! TCCGAAGTATCGGCAATACA+TGG + chr3_4:3450998-3451017 None:intergenic 45.0%
TCTTGTCTGATGTCCCACAA+TGG - chr3_4:3451024-3451043 Msa0485710:intron 45.0%
GCTAACTACTACCTCCATTG+TGG + chr3_4:3450858-3450877 None:intergenic 45.0%
ACCACAGTAGTGACACATGT+CGG + chr3_4:3451104-3451123 None:intergenic 45.0%
ATGTAAACACACGCTGACAC+AGG + chr3_4:3451284-3451303 None:intergenic 45.0%
TGACATGGATATGTCGACAC+AGG + chr3_4:3451503-3451522 None:intergenic 45.0%
GTGGTGCAGGAATCAACAAT+AGG - chr3_4:3451765-3451784 Msa0485710:intron 45.0%
GGTGCAGGAATCAACAATAG+GGG - chr3_4:3451767-3451786 Msa0485710:intron 45.0%
! TTTGAGATTTGGGACACAGC+AGG - chr3_4:3451837-3451856 Msa0485710:intron 45.0%
AATACATGGGAGCCAAGCTA+TGG + chr3_4:3451875-3451894 None:intergenic 45.0%
TTGGCTCCCATGTATTACAG+AGG - chr3_4:3451879-3451898 Msa0485710:CDS 45.0%
CAAAGATTGCCCAACGAAGT+AGG + chr3_4:3451979-3451998 None:intergenic 45.0%
ATTGAGACACAACTCAGACC+TGG - chr3_4:3452197-3452216 Msa0485710:intron 45.0%
CTTCTCACGAGCTAAGAAGT+GGG - chr3_4:3452575-3452594 Msa0485710:intron 45.0%
GTCCAAGAGCTTCAAAAGCA+AGG - chr3_4:3452597-3452616 Msa0485710:intron 45.0%
!! GAAGGTACCATCCAGAAGTT+TGG + chr3_4:3452729-3452748 None:intergenic 45.0%
AACACGAGGGACATTGACAT+TGG + chr3_4:3452775-3452794 None:intergenic 45.0%
CAAGAGCCATGACCATGTTT+GGG + chr3_4:3453309-3453328 None:intergenic 45.0%
TGCTGGCAACAAATCTGACT+TGG - chr3_4:3453327-3453346 Msa0485710:CDS 45.0%
!! ATCACTTTTCCTGGACCTAC+TGG - chr3_4:3453834-3453853 Msa0485710:intron 45.0%
AGTGATAAACCAGTAGGTCC+AGG + chr3_4:3453846-3453865 None:intergenic 45.0%
CGTGTATATGCCGAGGAAAA+TGG - chr3_4:3453893-3453912 Msa0485710:intron 45.0%
AAGAAAGCTTCCCCACTCTT+CGG + chr3_4:3454089-3454108 None:intergenic 45.0%
! AGTGGGGAAGCTTTCTTTTG+AGG - chr3_4:3454092-3454111 Msa0485710:intron 45.0%
! GTGGGGAAGCTTTCTTTTGA+GGG - chr3_4:3454093-3454112 Msa0485710:intron 45.0%
CTCTAATTGGCTATTGGCCA+TGG - chr3_4:3454278-3454297 Msa0485710:intron 45.0%
AGGTTTGGATAACTGTGCCA+TGG + chr3_4:3454298-3454317 None:intergenic 45.0%
AGGCATGTCATGAGAAGGTT+TGG + chr3_4:3454313-3454332 None:intergenic 45.0%
GAACAAGGCATGTCATGAGA+AGG + chr3_4:3454318-3454337 None:intergenic 45.0%
TCACCAATCGCCTACATATG+TGG - chr3_4:3454634-3454653 Msa0485710:intron 45.0%
CACCAATCGCCTACATATGT+GGG - chr3_4:3454635-3454654 Msa0485710:intron 45.0%
GGACCCAAATATGCATCCAA+GGG + chr3_4:3455071-3455090 None:intergenic 45.0%
AGGACCCAAATATGCATCCA+AGG + chr3_4:3455072-3455091 None:intergenic 45.0%
! CTTCTTGGTGATATGGGTGC+TGG - chr3_4:3450435-3450454 Msa0485710:intron 50.0%
! GTCCGCATGTGTCATTACTG+TGG - chr3_4:3450916-3450935 Msa0485710:intron 50.0%
TGTCTGATGTCCCACAATGG+AGG - chr3_4:3451027-3451046 Msa0485710:intron 50.0%
! TCCGACATGTGTCACTACTG+TGG - chr3_4:3451100-3451119 Msa0485710:intron 50.0%
! TCAGTGGTGTCAGTGCTACA+CGG + chr3_4:3451238-3451257 None:intergenic 50.0%
!! GTAGCACTGACACCACTGAT+TGG - chr3_4:3451239-3451258 Msa0485710:intron 50.0%
CTGAACACGTCTCCAATCAG+TGG + chr3_4:3451254-3451273 None:intergenic 50.0%
AAACACACGCTGACACAGGT+TGG + chr3_4:3451280-3451299 None:intergenic 50.0%
GCACGGACACCTCTTAGATT+AGG - chr3_4:3451388-3451407 Msa0485710:intron 50.0%
ACACCGACAAGACACTGACA+TGG + chr3_4:3451518-3451537 None:intergenic 50.0%
!!! GAGCCCTGTTATTTTGTGGC+TGG - chr3_4:3451682-3451701 Msa0485710:intron 50.0%
GCTGCGCCTCTGTAATACAT+GGG + chr3_4:3451888-3451907 None:intergenic 50.0%
AGCTGCGCCTCTGTAATACA+TGG + chr3_4:3451889-3451908 None:intergenic 50.0%
! CTTTTGTCTTGTGGTTGGGC+TGG - chr3_4:3452465-3452484 Msa0485710:intron 50.0%
CCACTTCTTAGCTCGTGAGA+AGG + chr3_4:3452577-3452596 None:intergenic 50.0%
CCTTCTCACGAGCTAAGAAG+TGG - chr3_4:3452574-3452593 Msa0485710:intron 50.0%
!! TGTCTCTGGCTCTCTGTACA+TGG + chr3_4:3452974-3452993 None:intergenic 50.0%
AGGCAACCCAAACATGGTCA+TGG - chr3_4:3453300-3453319 Msa0485710:intron 50.0%
GCAAGAGCCATGACCATGTT+TGG + chr3_4:3453310-3453329 None:intergenic 50.0%
AACATGGTCATGGCTCTTGC+TGG - chr3_4:3453310-3453329 Msa0485710:CDS 50.0%
TGTGTGTGTGTGTGTGTGTG+TGG - chr3_4:3453651-3453670 Msa0485710:intron 50.0%
GTGTGTGTGTGTGTGTGTGT+GGG - chr3_4:3453652-3453671 Msa0485710:intron 50.0%
GATGCCCATTGCAGCATGAT+TGG - chr3_4:3453771-3453790 Msa0485710:intron 50.0%
ATTGGCTGCAGATTTGGCAG+AGG + chr3_4:3453936-3453955 None:intergenic 50.0%
AGAAAGCTTCCCCACTCTTC+GGG + chr3_4:3454088-3454107 None:intergenic 50.0%
ATTCAGACAACTGTGCGCCA+TGG + chr3_4:3454229-3454248 None:intergenic 50.0%
ACATAAAGCGGAAGCCTGCT+AGG + chr3_4:3454464-3454483 None:intergenic 50.0%
GTCCCACATATGTAGGCGAT+TGG + chr3_4:3454640-3454659 None:intergenic 50.0%
CAACAAGAACCATCCCTGCT+GGG + chr3_4:3454849-3454868 None:intergenic 50.0%
TCAACAAGAACCATCCCTGC+TGG + chr3_4:3454850-3454869 None:intergenic 50.0%
! CTTGTTGATAGACCTGCCGA+AGG - chr3_4:3454862-3454881 Msa0485710:intron 50.0%
ATAGACCTGCCGAAGGATCT+AGG - chr3_4:3454869-3454888 Msa0485710:intron 50.0%
TAGACCTGCCGAAGGATCTA+GGG - chr3_4:3454870-3454889 Msa0485710:intron 50.0%
! TCTTGTGGTTGGGCTGGATC+TGG - chr3_4:3452471-3452490 Msa0485710:intron 55.0%
GCTTGCAGGCAACCCAAACA+TGG - chr3_4:3453294-3453313 Msa0485710:intron 55.0%
TGTGTGTGTGTGTGTGTGGG+TGG - chr3_4:3453659-3453678 Msa0485710:intron 55.0%
GTGTGTGTGTGTGTGTGGGT+GGG - chr3_4:3453660-3453679 Msa0485710:intron 55.0%
TGTGTGTGTGTGTGTGGGTG+GGG - chr3_4:3453661-3453680 Msa0485710:intron 55.0%
GTGGGGGCTAGGTTTCAATC+TGG - chr3_4:3453683-3453702 Msa0485710:intron 55.0%
GGAAGCTCGTGTATATGCCG+AGG - chr3_4:3453886-3453905 Msa0485710:intron 55.0%
GGAAGCCTGCTAGGAACAGA+AGG + chr3_4:3454455-3454474 None:intergenic 55.0%
! TTTTGAGCTGGCTGAGCCCT+TGG + chr3_4:3454826-3454845 None:intergenic 55.0%
AGCCAGCTCAAAACCCAGCA+GGG - chr3_4:3454833-3454852 Msa0485710:intron 55.0%
!!! ATCCCTGCTGGGTTTTGAGC+TGG + chr3_4:3454838-3454857 None:intergenic 55.0%
AGCTCAAAACCCAGCAGGGA+TGG - chr3_4:3454837-3454856 Msa0485710:intron 55.0%
CGATGCAGCCCTAGATCCTT+CGG + chr3_4:3454881-3454900 None:intergenic 55.0%
TGTGTGTGTGTGGGTGGGGT+GGG - chr3_4:3453665-3453684 Msa0485710:intron 60.0%
CAGCCAGCTCAAAACCCAGC+AGG - chr3_4:3454832-3454851 Msa0485710:intron 60.0%
TTCCTGTGTCCGACGCGCGT+CGG - chr3_4:3451414-3451433 Msa0485710:intron 65.0%
AGACAGACACCGACGCGCGT+CGG + chr3_4:3451426-3451445 None:intergenic 65.0%
GTGTGTGTGTGTGGGTGGGG+TGG - chr3_4:3453664-3453683 Msa0485710:intron 65.0%
GTGTGTGTGTGGGTGGGGTG+GGG - chr3_4:3453666-3453685 Msa0485710:intron 65.0%
TGTGTGTGTGGGTGGGGTGG+GGG - chr3_4:3453667-3453686 Msa0485710:intron 65.0%
GCAGCCCTAGATCCTTCGGC+AGG + chr3_4:3454877-3454896 None:intergenic 65.0%
CACCGACGCGCGTCGGACAC+AGG + chr3_4:3451419-3451438 None:intergenic 75.0%
GTGTGGGTGGGGTGGGGGCT+AGG - chr3_4:3453672-3453691 Msa0485710:intron 75.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 3450080 3455169 3450080 ID=Msa0485710;Name=Msa0485710
chr3_4 mRNA 3450080 3455169 3450080 ID=Msa0485710-mRNA-1;Parent=Msa0485710;Name=Msa0485710-mRNA-1;_AED=0.15;_eAED=0.15;_QI=235|1|1|1|1|1|7|257|200
chr3_4 exon 3450080 3450437 3450080 ID=Msa0485710-mRNA-1:exon:1261;Parent=Msa0485710-mRNA-1
chr3_4 exon 3451269 3451362 3451269 ID=Msa0485710-mRNA-1:exon:1260;Parent=Msa0485710-mRNA-1
chr3_4 exon 3451874 3451947 3451874 ID=Msa0485710-mRNA-1:exon:1259;Parent=Msa0485710-mRNA-1
chr3_4 exon 3452631 3452679 3452631 ID=Msa0485710-mRNA-1:exon:1258;Parent=Msa0485710-mRNA-1
chr3_4 exon 3453308 3453475 3453308 ID=Msa0485710-mRNA-1:exon:1257;Parent=Msa0485710-mRNA-1
chr3_4 exon 3454740 3454817 3454740 ID=Msa0485710-mRNA-1:exon:1256;Parent=Msa0485710-mRNA-1
chr3_4 exon 3454896 3455169 3454896 ID=Msa0485710-mRNA-1:exon:1255;Parent=Msa0485710-mRNA-1
chr3_4 five_prime_UTR 3454935 3455169 3454935 ID=Msa0485710-mRNA-1:five_prime_utr;Parent=Msa0485710-mRNA-1
chr3_4 CDS 3454896 3454934 3454896 ID=Msa0485710-mRNA-1:cds;Parent=Msa0485710-mRNA-1
chr3_4 CDS 3454740 3454817 3454740 ID=Msa0485710-mRNA-1:cds;Parent=Msa0485710-mRNA-1
chr3_4 CDS 3453308 3453475 3453308 ID=Msa0485710-mRNA-1:cds;Parent=Msa0485710-mRNA-1
chr3_4 CDS 3452631 3452679 3452631 ID=Msa0485710-mRNA-1:cds;Parent=Msa0485710-mRNA-1
chr3_4 CDS 3451874 3451947 3451874 ID=Msa0485710-mRNA-1:cds;Parent=Msa0485710-mRNA-1
chr3_4 CDS 3451269 3451362 3451269 ID=Msa0485710-mRNA-1:cds;Parent=Msa0485710-mRNA-1
chr3_4 CDS 3450337 3450437 3450337 ID=Msa0485710-mRNA-1:cds;Parent=Msa0485710-mRNA-1
chr3_4 three_prime_UTR 3450080 3450336 3450080 ID=Msa0485710-mRNA-1:three_prime_utr;Parent=Msa0485710-mRNA-1
Gene Sequence

>Msa0485710

ATGGCTACGATCGCACACAACAATCTCAATGCTAAATTGGTTCTTCTTGGTGATATGGGTGCTGGAAAATCTAGTCTTGTTTTAAGATTTGTCAAGGGTCAATTTCTCGAATTTCAGGAATCAACAATAGGGGCAGCATTTTTCTCTCAGACATTAGCAGTAAATGACGCAACGGTTAAATTTGAGATTTGGGACACAGCAGGACAAGAGAGATACCATAGCTTGGCTCCCATGTATTACAGAGGCGCAGCTGCTGCTATAATTGTCTATGACATCACTAGCTCAGATTCCTTCTCACGAGCTAAGAAGTGGGTCCAAGAGCTTCAAAAGCAAGGCAACCCAAACATGGTCATGGCTCTTGCTGGCAACAAATCTGACTTGGAAGATAAAAGAAAAGTGACAGCCGAAGAAGCTCGTGTATATGCCGAGGAAAATGGTTTGTTTTTCATGGAGACCTCTGCCAAATCTGCAGCCAATGTTAATGATGTATTTTATGAAATAGCTAAAAGATTACCAAGGGCTCAGCCAGCTCAAAACCCAGCAGGGATGGTTCTTGTTGATAGACCTGCCGAAGGATCTAGGGCTGCATCGTGTTGTTCATAA
Protein sequence

>Msa0485710

MATIAHNNLNAKLVLLGDMGAGKSSLVLRFVKGQFLEFQESTIGAAFFSQTLAVNDATVKFEIWDTAGQERYHSLAPMYYRGAAAAIIVYDITSSDSFSRAKKWVQELQKQGNPNMVMALAGNKSDLEDKRKVTAEEARVYAEENGLFFMETSAKSAANVNDVFYEIAKRLPRAQPAQNPAGMVLVDRPAEGSRAASCCS*