AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0662220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662220 MtrunA17_Chr4g0034761 92.708 96 5 1 1 96 11 104 2.09e-61 182
Msa0662220 MtrunA17_Chr5g0446661 60.215 93 30 2 1 87 1 92 1.01e-25 91.7
Msa0662220 MtrunA17_Chr3g0078771 61.538 78 24 1 1 72 1 78 2.44e-22 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662220 AT1G49310.1 42.254 71 37 2 8 74 12 82 7.49e-13 58.9

Find 29 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTTCTTCTTCTGCTTCT+TGG 0.153872 4_3:+74167265 None:intergenic
TTAATATCTTATATGTTATA+TGG 0.245081 4_3:-74167175 Msa0662220:three_prime_UTR
GAAAAGACTTGGGAGATTAT+TGG 0.261061 4_3:-74167421 Msa0662220:CDS
AATTAGATTTGTTAGTTTGA+TGG 0.285742 4_3:+74167673 None:intergenic
CTCCCCAGATGTATTGTGTT+TGG 0.312718 4_3:-74166931 Msa0662220:three_prime_UTR
ACAAGTTCTTTGATTGCTTC+AGG 0.324229 4_3:+74167371 None:intergenic
TTGTTGGCACCAGTTGAAAT+TGG 0.331468 4_3:-74167765 None:intergenic
AAATACATCTATAGATAAAA+AGG 0.351346 4_3:+74167073 None:intergenic
AACTCTGAACAAGAGCTTCA+TGG 0.354626 4_3:-74167227 Msa0662220:CDS
AATACCAAACACAATACATC+TGG 0.373946 4_3:+74166927 None:intergenic
GATACATCAATCAAATCTAT+TGG 0.418852 4_3:+74166995 None:intergenic
CTCTTATGCAAGAAAAGACT+TGG 0.442343 4_3:-74167432 Msa0662220:CDS
GAGTATGAGAGAAGCAAAGA+TGG 0.451907 4_3:+74167616 None:intergenic
GATTTGTTAGTTTGATGGCT+AGG 0.457950 4_3:+74167678 None:intergenic
TCTTTGATTGCTTCAGGCAT+AGG 0.472850 4_3:+74167377 None:intergenic
ACGGTTGTACCAATTTCAAC+TGG 0.472939 4_3:+74167756 None:intergenic
AGTCAAAAGACAGTGAAACT+TGG 0.478801 4_3:-74166897 Msa0662220:three_prime_UTR
GTGTGATATAATATGACATT+AGG 0.480243 4_3:+74167299 None:intergenic
TGATTGCTTCAGGCATAGGT+TGG 0.514568 4_3:+74167381 None:intergenic
TGGTACAACCGTGTATAAAG+TGG 0.539054 4_3:-74167745 Msa0662220:five_prime_UTR
AGAACTTGTTCAAAATCAAG+AGG 0.539159 4_3:-74167357 Msa0662220:CDS
TTTGATTGATGTATCTAGTG+TGG 0.552142 4_3:-74166987 Msa0662220:three_prime_UTR
ATACCAAACACAATACATCT+GGG 0.600413 4_3:+74166928 None:intergenic
AAAAGACAGTGAAACTTGGA+TGG 0.605833 4_3:-74166893 Msa0662220:three_prime_UTR
TCTTATGCAAGAAAAGACTT+GGG 0.611202 4_3:-74167431 Msa0662220:CDS
TAAAAGTCCACATCTTAGCA+AGG 0.615141 4_3:-74167651 Msa0662220:five_prime_UTR
GGTACAACCGTGTATAAAGT+GGG 0.640534 4_3:-74167744 Msa0662220:five_prime_UTR
AAAGGGGCCCACTTTATACA+CGG 0.655601 4_3:+74167737 None:intergenic
TACCAAACACAATACATCTG+GGG 0.678240 4_3:+74166929 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATATCTTATATGTTATA+TGG - chr4_3:74167462-74167481 Msa0662220:intron 10.0%
!! TTAATATCTTATATGTTATA+TGG - chr4_3:74167462-74167481 Msa0662220:intron 10.0%
!! ACAATATAGTATAAATTGAT+TGG + chr4_3:74167140-74167159 None:intergenic 15.0%
!!! AATTTTCTTTGTACTAAAAT+AGG - chr4_3:74167172-74167191 Msa0662220:three_prime_UTR 15.0%
!!! TATTTAAGGTTTATTTTGTA+GGG - chr4_3:74167497-74167516 Msa0662220:intron 15.0%
!! AAATACATCTATAGATAAAA+AGG + chr4_3:74167567-74167586 None:intergenic 15.0%
!!! TATCTATAGATGTATTTTTA+TGG - chr4_3:74167570-74167589 Msa0662220:intron 15.0%
!! ACAATATAGTATAAATTGAT+TGG + chr4_3:74167140-74167159 None:intergenic 15.0%
!!! AATTTTCTTTGTACTAAAAT+AGG - chr4_3:74167172-74167191 Msa0662220:three_prime_UTR 15.0%
!!! TATTTAAGGTTTATTTTGTA+GGG - chr4_3:74167497-74167516 Msa0662220:intron 15.0%
!! AAATACATCTATAGATAAAA+AGG + chr4_3:74167567-74167586 None:intergenic 15.0%
!!! TATCTATAGATGTATTTTTA+TGG - chr4_3:74167570-74167589 Msa0662220:intron 15.0%
!!! AATTAGATTTGTTAGTTTGA+TGG + chr4_3:74166967-74166986 None:intergenic 20.0%
!! TTAGTACAAAGAAAATTGAA+AGG + chr4_3:74167169-74167188 None:intergenic 20.0%
!! GGAAAAAAGATTAGTATTTA+AGG - chr4_3:74167483-74167502 Msa0662220:intron 20.0%
!!! GTATTTAAGGTTTATTTTGT+AGG - chr4_3:74167496-74167515 Msa0662220:intron 20.0%
!!! TAAAGTTGTATTTTTGTTGA+TGG - chr4_3:74167535-74167554 Msa0662220:intron 20.0%
!!! AATTAGATTTGTTAGTTTGA+TGG + chr4_3:74166967-74166986 None:intergenic 20.0%
!! TTAGTACAAAGAAAATTGAA+AGG + chr4_3:74167169-74167188 None:intergenic 20.0%
!! GGAAAAAAGATTAGTATTTA+AGG - chr4_3:74167483-74167502 Msa0662220:intron 20.0%
!!! GTATTTAAGGTTTATTTTGT+AGG - chr4_3:74167496-74167515 Msa0662220:intron 20.0%
!!! TAAAGTTGTATTTTTGTTGA+TGG - chr4_3:74167535-74167554 Msa0662220:intron 20.0%
!! TTCTTTTTATAGGCCAAAAA+GGG + chr4_3:74166920-74166939 None:intergenic 25.0%
!! TTTCTTTTTATAGGCCAAAA+AGG + chr4_3:74166921-74166940 None:intergenic 25.0%
!!! CTCTGACTATTTCTTTTTAT+AGG + chr4_3:74166930-74166949 None:intergenic 25.0%
! CTATAAAAAGAAATAGTCAG+AGG - chr4_3:74166928-74166947 Msa0662220:three_prime_UTR 25.0%
! GTGTGATATAATATGACATT+AGG + chr4_3:74167341-74167360 None:intergenic 25.0%
!!! TTTTTTGCTGCATCTATTTT+TGG - chr4_3:74167601-74167620 Msa0662220:CDS 25.0%
!!! TTTTTGCTGCATCTATTTTT+GGG - chr4_3:74167602-74167621 Msa0662220:CDS 25.0%
! GATACATCAATCAAATCTAT+TGG + chr4_3:74167645-74167664 None:intergenic 25.0%
!! TTCTTTTTATAGGCCAAAAA+GGG + chr4_3:74166920-74166939 None:intergenic 25.0%
!! TTTCTTTTTATAGGCCAAAA+AGG + chr4_3:74166921-74166940 None:intergenic 25.0%
!!! CTCTGACTATTTCTTTTTAT+AGG + chr4_3:74166930-74166949 None:intergenic 25.0%
! CTATAAAAAGAAATAGTCAG+AGG - chr4_3:74166928-74166947 Msa0662220:three_prime_UTR 25.0%
! GTGTGATATAATATGACATT+AGG + chr4_3:74167341-74167360 None:intergenic 25.0%
!!! TTTTTTGCTGCATCTATTTT+TGG - chr4_3:74167601-74167620 Msa0662220:CDS 25.0%
!!! TTTTTGCTGCATCTATTTTT+GGG - chr4_3:74167602-74167621 Msa0662220:CDS 25.0%
! GATACATCAATCAAATCTAT+TGG + chr4_3:74167645-74167664 None:intergenic 25.0%
! TCTTTTTATAGGCCAAAAAG+GGG + chr4_3:74166919-74166938 None:intergenic 30.0%
!!! ATTTTTTCCTTGCTAAGATG+TGG + chr4_3:74166996-74167015 None:intergenic 30.0%
TCTTATGCAAGAAAAGACTT+GGG - chr4_3:74167206-74167225 Msa0662220:exon 30.0%
! AGAACTTGTTCAAAATCAAG+AGG - chr4_3:74167280-74167299 Msa0662220:CDS 30.0%
CTTCATGGAACATAAAAACA+TGG - chr4_3:74167425-74167444 Msa0662220:CDS 30.0%
ATGGAACATAAAAACATGGT+TGG - chr4_3:74167429-74167448 Msa0662220:CDS 30.0%
TTTGATTGATGTATCTAGTG+TGG - chr4_3:74167650-74167669 Msa0662220:five_prime_UTR 30.0%
ATACCAAACACAATACATCT+GGG + chr4_3:74167712-74167731 None:intergenic 30.0%
AATACCAAACACAATACATC+TGG + chr4_3:74167713-74167732 None:intergenic 30.0%
! TCTTTTTATAGGCCAAAAAG+GGG + chr4_3:74166919-74166938 None:intergenic 30.0%
!!! ATTTTTTCCTTGCTAAGATG+TGG + chr4_3:74166996-74167015 None:intergenic 30.0%
TCTTATGCAAGAAAAGACTT+GGG - chr4_3:74167206-74167225 Msa0662220:exon 30.0%
! AGAACTTGTTCAAAATCAAG+AGG - chr4_3:74167280-74167299 Msa0662220:CDS 30.0%
CTTCATGGAACATAAAAACA+TGG - chr4_3:74167425-74167444 Msa0662220:CDS 30.0%
ATGGAACATAAAAACATGGT+TGG - chr4_3:74167429-74167448 Msa0662220:CDS 30.0%
TTTGATTGATGTATCTAGTG+TGG - chr4_3:74167650-74167669 Msa0662220:five_prime_UTR 30.0%
ATACCAAACACAATACATCT+GGG + chr4_3:74167712-74167731 None:intergenic 30.0%
AATACCAAACACAATACATC+TGG + chr4_3:74167713-74167732 None:intergenic 30.0%
!! GATTTGTTAGTTTGATGGCT+AGG + chr4_3:74166962-74166981 None:intergenic 35.0%
TAAAAGTCCACATCTTAGCA+AGG - chr4_3:74166986-74167005 Msa0662220:three_prime_UTR 35.0%
! CATACTCTTTTCTCTTCTTC+TGG - chr4_3:74167037-74167056 Msa0662220:three_prime_UTR 35.0%
! AGTCTTTTCTTGCATAAGAG+AGG + chr4_3:74167205-74167224 None:intergenic 35.0%
CTCTTATGCAAGAAAAGACT+TGG - chr4_3:74167205-74167224 Msa0662220:exon 35.0%
GAAAAGACTTGGGAGATTAT+TGG - chr4_3:74167216-74167235 Msa0662220:exon 35.0%
ACAAGTTCTTTGATTGCTTC+AGG + chr4_3:74167269-74167288 None:intergenic 35.0%
! TTGTTTCTTCTTCTGCTTCT+TGG + chr4_3:74167375-74167394 None:intergenic 35.0%
TACCAAACACAATACATCTG+GGG + chr4_3:74167711-74167730 None:intergenic 35.0%
AGTCAAAAGACAGTGAAACT+TGG - chr4_3:74167740-74167759 Msa0662220:five_prime_UTR 35.0%
AAAAGACAGTGAAACTTGGA+TGG - chr4_3:74167744-74167763 Msa0662220:five_prime_UTR 35.0%
GAAACTTGGATGGTATTTGA+TGG - chr4_3:74167754-74167773 Msa0662220:five_prime_UTR 35.0%
!! GATTTGTTAGTTTGATGGCT+AGG + chr4_3:74166962-74166981 None:intergenic 35.0%
TAAAAGTCCACATCTTAGCA+AGG - chr4_3:74166986-74167005 Msa0662220:three_prime_UTR 35.0%
! CATACTCTTTTCTCTTCTTC+TGG - chr4_3:74167037-74167056 Msa0662220:three_prime_UTR 35.0%
! AGTCTTTTCTTGCATAAGAG+AGG + chr4_3:74167205-74167224 None:intergenic 35.0%
CTCTTATGCAAGAAAAGACT+TGG - chr4_3:74167205-74167224 Msa0662220:exon 35.0%
GAAAAGACTTGGGAGATTAT+TGG - chr4_3:74167216-74167235 Msa0662220:exon 35.0%
ACAAGTTCTTTGATTGCTTC+AGG + chr4_3:74167269-74167288 None:intergenic 35.0%
! TTGTTTCTTCTTCTGCTTCT+TGG + chr4_3:74167375-74167394 None:intergenic 35.0%
TACCAAACACAATACATCTG+GGG + chr4_3:74167711-74167730 None:intergenic 35.0%
AGTCAAAAGACAGTGAAACT+TGG - chr4_3:74167740-74167759 Msa0662220:five_prime_UTR 35.0%
AAAAGACAGTGAAACTTGGA+TGG - chr4_3:74167744-74167763 Msa0662220:five_prime_UTR 35.0%
TGGTACAACCGTGTATAAAG+TGG - chr4_3:74166892-74166911 Msa0662220:three_prime_UTR 40.0%
GGTACAACCGTGTATAAAGT+GGG - chr4_3:74166893-74166912 Msa0662220:three_prime_UTR 40.0%
GAGTATGAGAGAAGCAAAGA+TGG + chr4_3:74167024-74167043 None:intergenic 40.0%
TCTTTGATTGCTTCAGGCAT+AGG + chr4_3:74167263-74167282 None:intergenic 40.0%
AACTCTGAACAAGAGCTTCA+TGG - chr4_3:74167410-74167429 Msa0662220:CDS 40.0%
TGGTACAACCGTGTATAAAG+TGG - chr4_3:74166892-74166911 Msa0662220:three_prime_UTR 40.0%
GGTACAACCGTGTATAAAGT+GGG - chr4_3:74166893-74166912 Msa0662220:three_prime_UTR 40.0%
GAGTATGAGAGAAGCAAAGA+TGG + chr4_3:74167024-74167043 None:intergenic 40.0%
TCTTTGATTGCTTCAGGCAT+AGG + chr4_3:74167263-74167282 None:intergenic 40.0%
AACTCTGAACAAGAGCTTCA+TGG - chr4_3:74167410-74167429 Msa0662220:CDS 40.0%
AAAGGGGCCCACTTTATACA+CGG + chr4_3:74166903-74166922 None:intergenic 45.0%
! GTATAAAGTGGGCCCCTTTT+TGG - chr4_3:74166904-74166923 Msa0662220:three_prime_UTR 45.0%
TGATTGCTTCAGGCATAGGT+TGG + chr4_3:74167259-74167278 None:intergenic 45.0%
CTCCCCAGATGTATTGTGTT+TGG - chr4_3:74167706-74167725 Msa0662220:five_prime_UTR 45.0%
AAAGGGGCCCACTTTATACA+CGG + chr4_3:74166903-74166922 None:intergenic 45.0%
! GTATAAAGTGGGCCCCTTTT+TGG - chr4_3:74166904-74166923 Msa0662220:three_prime_UTR 45.0%
TGATTGCTTCAGGCATAGGT+TGG + chr4_3:74167259-74167278 None:intergenic 45.0%
CTCCCCAGATGTATTGTGTT+TGG - chr4_3:74167706-74167725 Msa0662220:five_prime_UTR 45.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 74166884 74167775 74166884 ID=Msa0662220;Name=Msa0662220
chr4_3 mRNA 74166884 74167775 74166884 ID=Msa0662220-mRNA-1;Parent=Msa0662220;Name=Msa0662220-mRNA-1;_AED=0.08;_eAED=0.08;_QI=130|1|1|1|1|1|2|336|96
chr4_3 exon 74166884 74167465 74166884 ID=Msa0662220-mRNA-1:exon:22344;Parent=Msa0662220-mRNA-1
chr4_3 exon 74167601 74167775 74167601 ID=Msa0662220-mRNA-1:exon:22343;Parent=Msa0662220-mRNA-1
chr4_3 five_prime_UTR 74167646 74167775 74167646 ID=Msa0662220-mRNA-1:five_prime_utr;Parent=Msa0662220-mRNA-1
chr4_3 CDS 74167601 74167645 74167601 ID=Msa0662220-mRNA-1:cds;Parent=Msa0662220-mRNA-1
chr4_3 CDS 74167220 74167465 74167220 ID=Msa0662220-mRNA-1:cds;Parent=Msa0662220-mRNA-1
chr4_3 three_prime_UTR 74166884 74167219 74166884 ID=Msa0662220-mRNA-1:three_prime_utr;Parent=Msa0662220-mRNA-1
Gene Sequence

>Msa0662220

ATGAAGTCCATCTTTGCTTCTCTCATACTCTTTTCTCTTCTTCTGGTTGCTGACCTCTCTTATGCAAGAAAAGACTTGGGAGATTATTGGAAGAATATGATGAATGACCAACCTATGCCTGAAGCAATCAAAGAACTTGTTCAAAATCAAGAGGTTTCAGATGACTTTATTAGAGACTTTGATGTAAAGCCTAATGTCATATTATATCACACACATGCTGAATCCAAGAAGCAGAAGAAGAAACAACATGTCTTTGTGAAAAACTCTGAACAAGAGCTTCATGGAACATAA

Protein sequence

>Msa0662220

MKSIFASLILFSLLLVADLSYARKDLGDYWKNMMNDQPMPEAIKELVQNQEVSDDFIRDFDVKPNVILYHTHAESKKQKKKQHVFVKNSEQELHGT*