AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0320230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320230 MtrunA17_Chr2g0295141 97.452 157 4 0 1 157 1 157 2.28e-108 305
Msa0320230 MtrunA17_Chr2g0295091 93.631 157 10 0 1 157 1 157 3.56e-104 295
Msa0320230 MtrunA17_Chr2g0295104 92.357 157 12 0 1 157 1 157 4.90e-103 291
Msa0320230 MtrunA17_Chr2g0295124 88.535 157 18 0 1 157 1 157 1.80e-98 280
Msa0320230 MtrunA17_Chr6g0462311 64.968 157 55 0 1 157 1 157 6.39e-70 208
Msa0320230 MtrunA17_Chr4g0067951 64.968 157 55 0 1 157 1 157 2.57e-69 206
Msa0320230 MtrunA17_Chr2g0295021 64.968 157 55 0 1 157 1 157 1.19e-68 204
Msa0320230 MtrunA17_Chr2g0295001 64.968 157 55 0 1 157 1 157 1.19e-68 204
Msa0320230 MtrunA17_Chr2g0295051 63.057 157 58 0 1 157 1 157 7.23e-67 200
Msa0320230 MtrunA17_Chr2g0295064 63.057 157 58 0 1 157 68 224 6.19e-66 200
Msa0320230 MtrunA17_Chr2g0295041 57.325 157 67 0 1 157 1 157 1.75e-60 184
Msa0320230 MtrunA17_Chr6g0462291 63.571 140 51 0 1 140 1 140 1.05e-59 182
Msa0320230 MtrunA17_Chr2g0295081 47.134 157 83 0 1 157 1 157 5.68e-48 152
Msa0320230 MtrunA17_Chr4g0067921 42.857 161 85 5 1 157 1 158 1.58e-32 113
Msa0320230 MtrunA17_Chr2g0295211 32.692 156 104 1 1 155 1 156 8.48e-31 108
Msa0320230 MtrunA17_Chr4g0067841 33.974 156 102 1 1 155 1 156 2.42e-30 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTATACTTGGTTTAATTT+TGG 0.203783 2_4:+20499470 Msa0320230:three_prime_UTR
AGACATGGATAACTTCTTAA+TGG 0.262600 2_4:-20498616 Msa0320220:intron
TATACATTTACTTAATAATC+AGG 0.319660 2_4:-20499454 Msa0320220:intron
AATAATTGAGGTTTAAGATA+TGG 0.327446 2_4:+20499518 Msa0320230:three_prime_UTR
GGCTATTGAGGGCTACGTTT+TGG 0.335215 2_4:+20499426 Msa0320230:CDS
GATAACTTCTTAATGGTTCC+TGG 0.335652 2_4:-20498609 Msa0320220:intron
GGTTGTGTCGTGTCGTTTCA+TGG 0.339282 2_4:+20499491 Msa0320230:three_prime_UTR
TTCAACAATTTCAACACTTT+GGG 0.350751 2_4:-20498574 Msa0320220:intron
TACAGCTTAGTAGGAGGAAC+AGG 0.356588 2_4:+20499238 Msa0320230:CDS
CTTCAACAATTTCAACACTT+TGG 0.364915 2_4:-20498575 Msa0320220:intron
GAAACTAAGGCCAGAGGAAC+TGG 0.366866 2_4:+20499394 Msa0320230:CDS
TGGGCAGCAGAAATCACCTT+TGG 0.387864 2_4:-20498555 Msa0320220:intron
ATTACATGTGTGTGGGGCTG+TGG 0.393490 2_4:+20499548 Msa0320230:three_prime_UTR
AGAGCTTTGTAGAGTTTAGC+TGG 0.394931 2_4:-20498507 Msa0320220:intron
TGCAGTAGATGAGGCAAACT+TGG 0.396721 2_4:+20499207 Msa0320230:CDS
GTTGAAATTGTTGAAGGAAA+TGG 0.402462 2_4:+20498582 Msa0320230:CDS
AAATCTAAGTGATGATGTTA+AGG 0.406482 2_4:+20499646 Msa0320230:three_prime_UTR
TTATCTTGATATATATATCA+TGG 0.417043 2_4:+20498443 Msa0320230:exon
TTATTAAGTAAATGTATACT+TGG 0.430237 2_4:+20499459 Msa0320230:exon
GGATACAACTACAGCTTAGT+AGG 0.431725 2_4:+20499229 Msa0320230:CDS
CTTGATAAGTCCAGTTCCTC+TGG 0.436052 2_4:-20499404 Msa0320220:intron
AGATAGAGCTGCATCACCTT+TGG 0.442558 2_4:-20499356 Msa0320220:intron
GGGCAGCAGAAATCACCTTT+GGG 0.449440 2_4:-20498554 Msa0320220:intron
TCCAAAACACCACTCAAAAC+CGG 0.454809 2_4:-20499597 Msa0320220:intron
AAAATTGTGGCTGGCTCTGA+TGG 0.492741 2_4:+20499298 Msa0320230:CDS
TTGTTTAATTACATGTGTGT+GGG 0.500989 2_4:+20499541 Msa0320230:three_prime_UTR
TAATTTGTGTAGCACAAAGT+TGG 0.501990 2_4:-20499182 Msa0320220:intron
CAAAGTGTTGAAATTGTTGA+AGG 0.502542 2_4:+20498576 Msa0320230:CDS
TGGACTTATCAAGGCTATTG+AGG 0.507134 2_4:+20499414 Msa0320230:CDS
CAGAGGAACTGGACTTATCA+AGG 0.509407 2_4:+20499405 Msa0320230:CDS
TATCTTGATATATATATCAT+GGG 0.511953 2_4:+20498444 Msa0320230:exon
GTTGTTTAATTACATGTGTG+TGG 0.512662 2_4:+20499540 Msa0320230:three_prime_UTR
TTTCATGGGAATAATAATTG+AGG 0.518683 2_4:+20499506 Msa0320230:three_prime_UTR
TTTCACTGTAATCTTAGCAA+TGG 0.523574 2_4:-20499326 Msa0320220:intron
GTTGTGTCGTGTCGTTTCAT+GGG 0.524521 2_4:+20499492 Msa0320230:three_prime_UTR
GGACTTATCAAGGCTATTGA+GGG 0.531505 2_4:+20499415 Msa0320230:CDS
CTCTGGCCTTAGTTTCATCA+CGG 0.532674 2_4:-20499387 Msa0320220:intron
TGCAGTCCGTGATGAAACTA+AGG 0.533307 2_4:+20499381 Msa0320230:CDS
TTACATGTGTGTGGGGCTGT+GGG 0.548075 2_4:+20499549 Msa0320230:three_prime_UTR
GTTGAAGGAAATGGAGGACC+AGG 0.585895 2_4:+20498591 Msa0320230:CDS
CAAATTAGATGCAGTAGATG+AGG 0.587202 2_4:+20499198 Msa0320230:CDS
ATTGTGGCTGGCTCTGATGG+TGG 0.588345 2_4:+20499301 Msa0320230:CDS
ATTTATTGAAGAAATACAGA+TGG 0.594988 2_4:-20499676 Msa0320220:intron
TGTTTAATTACATGTGTGTG+GGG 0.595453 2_4:+20499542 Msa0320230:three_prime_UTR
GCAGTAGATGAGGCAAACTT+GGG 0.598362 2_4:+20499208 Msa0320230:CDS
GTAGAGTTTAGCTGGAGCCA+CGG 0.602676 2_4:-20498499 Msa0320220:intron
TGCTGATGAAATCGTCCCAA+AGG 0.606260 2_4:+20498539 Msa0320230:CDS
CTTAGTAGGAGGAACAGGGT+TGG 0.609437 2_4:+20499243 Msa0320230:CDS
ATATGCAAGAAGAAAATGAA+AGG 0.609467 2_4:-20498411 Msa0320220:intron
TTGATGCTGTGAGGTAGTGC+CGG 0.617416 2_4:+20499578 Msa0320230:three_prime_UTR
CTAGTATATAACTTGTGATG+CGG 0.618776 2_4:-20498366 Msa0320220:intron
AAGAAGTTATCCATGTCTGA+AGG 0.619942 2_4:+20498621 Msa0320230:CDS
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2_4:+20498585 Msa0320230:CDS
TACAACTACAGCTTAGTAGG+AGG 0.637578 2_4:+20499232 Msa0320230:CDS
TCTTAAATTACCTTCAGACA+TGG 0.649758 2_4:-20498631 Msa0320220:intron
GAGCTGCATCACCTTTGGTG+TGG 0.654393 2_4:-20499351 Msa0320220:intron
ACAGTGAAATACCACACCAA+AGG 0.661287 2_4:+20499340 Msa0320230:CDS
GGGCTATGTTTGATGCTGTG+AGG 0.685047 2_4:+20499569 Msa0320230:three_prime_UTR
CGTGATGAAACTAAGGCCAG+AGG 0.688505 2_4:+20499388 Msa0320230:CDS
TGATGAACATGTATCAACCG+TGG 0.718349 2_4:+20498482 Msa0320230:CDS
ACAGCTTAGTAGGAGGAACA+GGG 0.723853 2_4:+20499239 Msa0320230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATAAATAAATTATAA+TGG + chr2_4:20499139-20499158 Msa0320230:intron 0.0%
!! ATTATATAAATAAATTATAA+TGG + chr2_4:20499139-20499158 Msa0320230:intron 0.0%
!!! AAATAAAAGTATAACATAAA+TGG - chr2_4:20498942-20498961 Msa0320220:intron 10.0%
!!! AAATAAAAGTATAACATAAA+TGG - chr2_4:20498942-20498961 Msa0320220:intron 10.0%
!! TTATCTTGATATATATATCA+TGG + chr2_4:20498443-20498462 Msa0320230:exon 15.0%
!! TATCTTGATATATATATCAT+GGG + chr2_4:20498444-20498463 Msa0320230:exon 15.0%
!!! TTTGTCAATTTTTAAGTAAA+AGG - chr2_4:20498777-20498796 Msa0320220:intron 15.0%
!!! GAATTTAACTTACTTTTATT+TGG - chr2_4:20498867-20498886 Msa0320220:intron 15.0%
!!! ACATGAATCATTTTTTTTTA+AGG - chr2_4:20499038-20499057 Msa0320220:intron 15.0%
!! TATACATTTACTTAATAATC+AGG - chr2_4:20499457-20499476 Msa0320220:intron 15.0%
!! TTATTAAGTAAATGTATACT+TGG + chr2_4:20499459-20499478 Msa0320230:exon 15.0%
!! TTATCTTGATATATATATCA+TGG + chr2_4:20498443-20498462 Msa0320230:exon 15.0%
!! TATCTTGATATATATATCAT+GGG + chr2_4:20498444-20498463 Msa0320230:exon 15.0%
!!! TTTGTCAATTTTTAAGTAAA+AGG - chr2_4:20498777-20498796 Msa0320220:intron 15.0%
!!! GAATTTAACTTACTTTTATT+TGG - chr2_4:20498867-20498886 Msa0320220:intron 15.0%
!!! ACATGAATCATTTTTTTTTA+AGG - chr2_4:20499038-20499057 Msa0320220:intron 15.0%
!! TATACATTTACTTAATAATC+AGG - chr2_4:20499457-20499476 Msa0320220:intron 15.0%
!! TTATTAAGTAAATGTATACT+TGG + chr2_4:20499459-20499478 Msa0320230:exon 15.0%
!!! ATGTATACTTGGTTTAATTT+TGG + chr2_4:20499470-20499489 Msa0320230:three_prime_UTR 20.0%
!!! AATAATTGAGGTTTAAGATA+TGG + chr2_4:20499518-20499537 Msa0320230:three_prime_UTR 20.0%
!! ATTTATTGAAGAAATACAGA+TGG - chr2_4:20499679-20499698 Msa0320220:intron 20.0%
!!! ATGTATACTTGGTTTAATTT+TGG + chr2_4:20499470-20499489 Msa0320230:three_prime_UTR 20.0%
!!! AATAATTGAGGTTTAAGATA+TGG + chr2_4:20499518-20499537 Msa0320230:three_prime_UTR 20.0%
!! ATTTATTGAAGAAATACAGA+TGG - chr2_4:20499679-20499698 Msa0320220:intron 20.0%
! ATATGCAAGAAGAAAATGAA+AGG - chr2_4:20498414-20498433 Msa0320220:intron 25.0%
! TTCAACAATTTCAACACTTT+GGG - chr2_4:20498577-20498596 Msa0320220:intron 25.0%
! AATAGAATCTTGAGAAATGA+AGG + chr2_4:20498966-20498985 Msa0320230:intron 25.0%
! ATAATTATTAGACATACTCC+TGG - chr2_4:20499012-20499031 Msa0320220:intron 25.0%
!! AGGAGTATGTCTAATAATTA+TGG + chr2_4:20499011-20499030 Msa0320230:intron 25.0%
! TTTCATGGGAATAATAATTG+AGG + chr2_4:20499506-20499525 Msa0320230:three_prime_UTR 25.0%
! TTGTTTAATTACATGTGTGT+GGG + chr2_4:20499541-20499560 Msa0320230:three_prime_UTR 25.0%
!! AAATCTAAGTGATGATGTTA+AGG + chr2_4:20499646-20499665 Msa0320230:three_prime_UTR 25.0%
! ATATGCAAGAAGAAAATGAA+AGG - chr2_4:20498414-20498433 Msa0320220:intron 25.0%
! TTCAACAATTTCAACACTTT+GGG - chr2_4:20498577-20498596 Msa0320220:intron 25.0%
! AATAGAATCTTGAGAAATGA+AGG + chr2_4:20498966-20498985 Msa0320230:intron 25.0%
! ATAATTATTAGACATACTCC+TGG - chr2_4:20499012-20499031 Msa0320220:intron 25.0%
!! AGGAGTATGTCTAATAATTA+TGG + chr2_4:20499011-20499030 Msa0320230:intron 25.0%
! TTTCATGGGAATAATAATTG+AGG + chr2_4:20499506-20499525 Msa0320230:three_prime_UTR 25.0%
! TTGTTTAATTACATGTGTGT+GGG + chr2_4:20499541-20499560 Msa0320230:three_prime_UTR 25.0%
!! AAATCTAAGTGATGATGTTA+AGG + chr2_4:20499646-20499665 Msa0320230:three_prime_UTR 25.0%
CTTCAACAATTTCAACACTT+TGG - chr2_4:20498578-20498597 Msa0320220:intron 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + chr2_4:20498576-20498595 Msa0320230:CDS 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + chr2_4:20498582-20498601 Msa0320230:CDS 30.0%
AGACATGGATAACTTCTTAA+TGG - chr2_4:20498619-20498638 Msa0320220:intron 30.0%
TCTTAAATTACCTTCAGACA+TGG - chr2_4:20498634-20498653 Msa0320220:intron 30.0%
!! TGTAAAATTGTTTTACACCG+AGG + chr2_4:20498709-20498728 Msa0320230:intron 30.0%
! TAATTTGTGTAGCACAAAGT+TGG - chr2_4:20499185-20499204 Msa0320220:intron 30.0%
TGAATTCGAGACAAAAATTG+TGG + chr2_4:20499285-20499304 Msa0320230:CDS 30.0%
TTTCACTGTAATCTTAGCAA+TGG - chr2_4:20499329-20499348 Msa0320220:intron 30.0%
GTTGTTTAATTACATGTGTG+TGG + chr2_4:20499540-20499559 Msa0320230:three_prime_UTR 30.0%
TGTTTAATTACATGTGTGTG+GGG + chr2_4:20499542-20499561 Msa0320230:three_prime_UTR 30.0%
!! TGTTTTGGATGAATTTGGTT+GGG + chr2_4:20499611-20499630 Msa0320230:three_prime_UTR 30.0%
! CTAGTATATAACTTGTGATG+CGG - chr2_4:20498369-20498388 Msa0320220:intron 30.0%
CTTCAACAATTTCAACACTT+TGG - chr2_4:20498578-20498597 Msa0320220:intron 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + chr2_4:20498576-20498595 Msa0320230:CDS 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + chr2_4:20498582-20498601 Msa0320230:CDS 30.0%
AGACATGGATAACTTCTTAA+TGG - chr2_4:20498619-20498638 Msa0320220:intron 30.0%
TCTTAAATTACCTTCAGACA+TGG - chr2_4:20498634-20498653 Msa0320220:intron 30.0%
!! TGTAAAATTGTTTTACACCG+AGG + chr2_4:20498709-20498728 Msa0320230:intron 30.0%
! TAATTTGTGTAGCACAAAGT+TGG - chr2_4:20499185-20499204 Msa0320220:intron 30.0%
TGAATTCGAGACAAAAATTG+TGG + chr2_4:20499285-20499304 Msa0320230:CDS 30.0%
TTTCACTGTAATCTTAGCAA+TGG - chr2_4:20499329-20499348 Msa0320220:intron 30.0%
GTTGTTTAATTACATGTGTG+TGG + chr2_4:20499540-20499559 Msa0320230:three_prime_UTR 30.0%
TGTTTAATTACATGTGTGTG+GGG + chr2_4:20499542-20499561 Msa0320230:three_prime_UTR 30.0%
!! TGTTTTGGATGAATTTGGTT+GGG + chr2_4:20499611-20499630 Msa0320230:three_prime_UTR 30.0%
GAAATTGTTGAAGGAAATGG+AGG + chr2_4:20498585-20498604 Msa0320230:CDS 35.0%
GATAACTTCTTAATGGTTCC+TGG - chr2_4:20498612-20498631 Msa0320220:intron 35.0%
AAGAAGTTATCCATGTCTGA+AGG + chr2_4:20498621-20498640 Msa0320230:CDS 35.0%
! TACACAATTCTTTGTTGTGC+TGG - chr2_4:20498750-20498769 Msa0320220:intron 35.0%
ATAATACATAAGCTCGTTCC+AGG + chr2_4:20498991-20499010 Msa0320230:intron 35.0%
TATAATGGCTGTATTGCAGA+TGG + chr2_4:20499154-20499173 Msa0320230:intron 35.0%
CAAATTAGATGCAGTAGATG+AGG + chr2_4:20499198-20499217 Msa0320230:CDS 35.0%
!! AGTGGTGTTTTGGATGAATT+TGG + chr2_4:20499606-20499625 Msa0320230:three_prime_UTR 35.0%
!! GTGTTTTGGATGAATTTGGT+TGG + chr2_4:20499610-20499629 Msa0320230:three_prime_UTR 35.0%
GAAATTGTTGAAGGAAATGG+AGG + chr2_4:20498585-20498604 Msa0320230:CDS 35.0%
GATAACTTCTTAATGGTTCC+TGG - chr2_4:20498612-20498631 Msa0320220:intron 35.0%
AAGAAGTTATCCATGTCTGA+AGG + chr2_4:20498621-20498640 Msa0320230:CDS 35.0%
! TACACAATTCTTTGTTGTGC+TGG - chr2_4:20498750-20498769 Msa0320220:intron 35.0%
ATAATACATAAGCTCGTTCC+AGG + chr2_4:20498991-20499010 Msa0320230:intron 35.0%
TATAATGGCTGTATTGCAGA+TGG + chr2_4:20499154-20499173 Msa0320230:intron 35.0%
CAAATTAGATGCAGTAGATG+AGG + chr2_4:20499198-20499217 Msa0320230:CDS 35.0%
!! AGTGGTGTTTTGGATGAATT+TGG + chr2_4:20499606-20499625 Msa0320230:three_prime_UTR 35.0%
!! GTGTTTTGGATGAATTTGGT+TGG + chr2_4:20499610-20499629 Msa0320230:three_prime_UTR 35.0%
TGATGAACATGTATCAACCG+TGG + chr2_4:20498482-20498501 Msa0320230:CDS 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG - chr2_4:20498510-20498529 Msa0320220:intron 40.0%
CGTGACATATTGAAGAGTTG+TGG + chr2_4:20498802-20498821 Msa0320230:intron 40.0%
GGATACAACTACAGCTTAGT+AGG + chr2_4:20499229-20499248 Msa0320230:CDS 40.0%
TACAACTACAGCTTAGTAGG+AGG + chr2_4:20499232-20499251 Msa0320230:CDS 40.0%
TTCGAGACAAAAATTGTGGC+TGG + chr2_4:20499289-20499308 Msa0320230:CDS 40.0%
ACAGTGAAATACCACACCAA+AGG + chr2_4:20499340-20499359 Msa0320230:CDS 40.0%
TGGACTTATCAAGGCTATTG+AGG + chr2_4:20499414-20499433 Msa0320230:CDS 40.0%
GGACTTATCAAGGCTATTGA+GGG + chr2_4:20499415-20499434 Msa0320230:CDS 40.0%
TCCAAAACACCACTCAAAAC+CGG - chr2_4:20499600-20499619 Msa0320220:intron 40.0%
TGATGAACATGTATCAACCG+TGG + chr2_4:20498482-20498501 Msa0320230:CDS 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG - chr2_4:20498510-20498529 Msa0320220:intron 40.0%
CGTGACATATTGAAGAGTTG+TGG + chr2_4:20498802-20498821 Msa0320230:intron 40.0%
GGATACAACTACAGCTTAGT+AGG + chr2_4:20499229-20499248 Msa0320230:CDS 40.0%
TACAACTACAGCTTAGTAGG+AGG + chr2_4:20499232-20499251 Msa0320230:CDS 40.0%
TTCGAGACAAAAATTGTGGC+TGG + chr2_4:20499289-20499308 Msa0320230:CDS 40.0%
ACAGTGAAATACCACACCAA+AGG + chr2_4:20499340-20499359 Msa0320230:CDS 40.0%
TGGACTTATCAAGGCTATTG+AGG + chr2_4:20499414-20499433 Msa0320230:CDS 40.0%
GGACTTATCAAGGCTATTGA+GGG + chr2_4:20499415-20499434 Msa0320230:CDS 40.0%
TCCAAAACACCACTCAAAAC+CGG - chr2_4:20499600-20499619 Msa0320220:intron 40.0%
TGCTGATGAAATCGTCCCAA+AGG + chr2_4:20498539-20498558 Msa0320230:CDS 45.0%
!! GGCGAATTTTGATCGATCCT+CGG - chr2_4:20498729-20498748 Msa0320220:intron 45.0%
TGCAGTAGATGAGGCAAACT+TGG + chr2_4:20499207-20499226 Msa0320230:CDS 45.0%
GCAGTAGATGAGGCAAACTT+GGG + chr2_4:20499208-20499227 Msa0320230:CDS 45.0%
TACAGCTTAGTAGGAGGAAC+AGG + chr2_4:20499238-20499257 Msa0320230:CDS 45.0%
ACAGCTTAGTAGGAGGAACA+GGG + chr2_4:20499239-20499258 Msa0320230:CDS 45.0%
!! AAAATTGTGGCTGGCTCTGA+TGG + chr2_4:20499298-20499317 Msa0320230:CDS 45.0%
AGATAGAGCTGCATCACCTT+TGG - chr2_4:20499359-20499378 Msa0320220:intron 45.0%
TGCAGTCCGTGATGAAACTA+AGG + chr2_4:20499381-20499400 Msa0320230:CDS 45.0%
!! CTCTGGCCTTAGTTTCATCA+CGG - chr2_4:20499390-20499409 Msa0320220:intron 45.0%
CTTGATAAGTCCAGTTCCTC+TGG - chr2_4:20499407-20499426 Msa0320220:intron 45.0%
CAGAGGAACTGGACTTATCA+AGG + chr2_4:20499405-20499424 Msa0320230:CDS 45.0%
!! GTTGTGTCGTGTCGTTTCAT+GGG + chr2_4:20499492-20499511 Msa0320230:three_prime_UTR 45.0%
TGCTGATGAAATCGTCCCAA+AGG + chr2_4:20498539-20498558 Msa0320230:CDS 45.0%
!! GGCGAATTTTGATCGATCCT+CGG - chr2_4:20498729-20498748 Msa0320220:intron 45.0%
TGCAGTAGATGAGGCAAACT+TGG + chr2_4:20499207-20499226 Msa0320230:CDS 45.0%
GCAGTAGATGAGGCAAACTT+GGG + chr2_4:20499208-20499227 Msa0320230:CDS 45.0%
TACAGCTTAGTAGGAGGAAC+AGG + chr2_4:20499238-20499257 Msa0320230:CDS 45.0%
ACAGCTTAGTAGGAGGAACA+GGG + chr2_4:20499239-20499258 Msa0320230:CDS 45.0%
!! AAAATTGTGGCTGGCTCTGA+TGG + chr2_4:20499298-20499317 Msa0320230:CDS 45.0%
AGATAGAGCTGCATCACCTT+TGG - chr2_4:20499359-20499378 Msa0320220:intron 45.0%
TGCAGTCCGTGATGAAACTA+AGG + chr2_4:20499381-20499400 Msa0320230:CDS 45.0%
!! CTCTGGCCTTAGTTTCATCA+CGG - chr2_4:20499390-20499409 Msa0320220:intron 45.0%
CTTGATAAGTCCAGTTCCTC+TGG - chr2_4:20499407-20499426 Msa0320220:intron 45.0%
CAGAGGAACTGGACTTATCA+AGG + chr2_4:20499405-20499424 Msa0320230:CDS 45.0%
!! GTTGTGTCGTGTCGTTTCAT+GGG + chr2_4:20499492-20499511 Msa0320230:three_prime_UTR 45.0%
GTAGAGTTTAGCTGGAGCCA+CGG - chr2_4:20498502-20498521 Msa0320220:intron 50.0%
GGGCAGCAGAAATCACCTTT+GGG - chr2_4:20498557-20498576 Msa0320220:intron 50.0%
TGGGCAGCAGAAATCACCTT+TGG - chr2_4:20498558-20498577 Msa0320220:intron 50.0%
GTTGAAGGAAATGGAGGACC+AGG + chr2_4:20498591-20498610 Msa0320230:CDS 50.0%
CTTAGTAGGAGGAACAGGGT+TGG + chr2_4:20499243-20499262 Msa0320230:CDS 50.0%
CGTGATGAAACTAAGGCCAG+AGG + chr2_4:20499388-20499407 Msa0320230:CDS 50.0%
GAAACTAAGGCCAGAGGAAC+TGG + chr2_4:20499394-20499413 Msa0320230:CDS 50.0%
! GGCTATTGAGGGCTACGTTT+TGG + chr2_4:20499426-20499445 Msa0320230:CDS 50.0%
!! GGTTGTGTCGTGTCGTTTCA+TGG + chr2_4:20499491-20499510 Msa0320230:three_prime_UTR 50.0%
ATTACATGTGTGTGGGGCTG+TGG + chr2_4:20499548-20499567 Msa0320230:three_prime_UTR 50.0%
TTACATGTGTGTGGGGCTGT+GGG + chr2_4:20499549-20499568 Msa0320230:three_prime_UTR 50.0%
GGGCTATGTTTGATGCTGTG+AGG + chr2_4:20499569-20499588 Msa0320230:three_prime_UTR 50.0%
TTGATGCTGTGAGGTAGTGC+CGG + chr2_4:20499578-20499597 Msa0320230:three_prime_UTR 50.0%
!!! GCCGGTTTTGAGTGGTGTTT+TGG + chr2_4:20499596-20499615 Msa0320230:three_prime_UTR 50.0%
GTAGAGTTTAGCTGGAGCCA+CGG - chr2_4:20498502-20498521 Msa0320220:intron 50.0%
GGGCAGCAGAAATCACCTTT+GGG - chr2_4:20498557-20498576 Msa0320220:intron 50.0%
TGGGCAGCAGAAATCACCTT+TGG - chr2_4:20498558-20498577 Msa0320220:intron 50.0%
GTTGAAGGAAATGGAGGACC+AGG + chr2_4:20498591-20498610 Msa0320230:CDS 50.0%
CTTAGTAGGAGGAACAGGGT+TGG + chr2_4:20499243-20499262 Msa0320230:CDS 50.0%
CGTGATGAAACTAAGGCCAG+AGG + chr2_4:20499388-20499407 Msa0320230:CDS 50.0%
GAAACTAAGGCCAGAGGAAC+TGG + chr2_4:20499394-20499413 Msa0320230:CDS 50.0%
! GGCTATTGAGGGCTACGTTT+TGG + chr2_4:20499426-20499445 Msa0320230:CDS 50.0%
!! GGTTGTGTCGTGTCGTTTCA+TGG + chr2_4:20499491-20499510 Msa0320230:three_prime_UTR 50.0%
ATTACATGTGTGTGGGGCTG+TGG + chr2_4:20499548-20499567 Msa0320230:three_prime_UTR 50.0%
TTACATGTGTGTGGGGCTGT+GGG + chr2_4:20499549-20499568 Msa0320230:three_prime_UTR 50.0%
GGGCTATGTTTGATGCTGTG+AGG + chr2_4:20499569-20499588 Msa0320230:three_prime_UTR 50.0%
TTGATGCTGTGAGGTAGTGC+CGG + chr2_4:20499578-20499597 Msa0320230:three_prime_UTR 50.0%
!!! GCCGGTTTTGAGTGGTGTTT+TGG + chr2_4:20499596-20499615 Msa0320230:three_prime_UTR 50.0%
!! ATTGTGGCTGGCTCTGATGG+TGG + chr2_4:20499301-20499320 Msa0320230:CDS 55.0%
GAGCTGCATCACCTTTGGTG+TGG - chr2_4:20499354-20499373 Msa0320220:intron 55.0%
!!! GAGGTAGTGCCGGTTTTGAG+TGG + chr2_4:20499588-20499607 Msa0320230:three_prime_UTR 55.0%
!! ATTGTGGCTGGCTCTGATGG+TGG + chr2_4:20499301-20499320 Msa0320230:CDS 55.0%
GAGCTGCATCACCTTTGGTG+TGG - chr2_4:20499354-20499373 Msa0320220:intron 55.0%
!!! GAGGTAGTGCCGGTTTTGAG+TGG + chr2_4:20499588-20499607 Msa0320230:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 20498368 20499763 20498368 ID=Msa0320230;Name=Msa0320230
chr2_4 mRNA 20498368 20499763 20498368 ID=Msa0320230-mRNA-1;Parent=Msa0320230;Name=Msa0320230-mRNA-1;_AED=0.28;_eAED=0.28;_QI=94|1|1|1|1|1|2|298|157
chr2_4 exon 20498368 20498642 20498368 ID=Msa0320230-mRNA-1:exon:7548;Parent=Msa0320230-mRNA-1
chr2_4 exon 20499173 20499763 20499173 ID=Msa0320230-mRNA-1:exon:7549;Parent=Msa0320230-mRNA-1
chr2_4 five_prime_UTR 20498368 20498461 20498368 ID=Msa0320230-mRNA-1:five_prime_utr;Parent=Msa0320230-mRNA-1
chr2_4 CDS 20498462 20498642 20498462 ID=Msa0320230-mRNA-1:cds;Parent=Msa0320230-mRNA-1
chr2_4 CDS 20499173 20499465 20499173 ID=Msa0320230-mRNA-1:cds;Parent=Msa0320230-mRNA-1
chr2_4 three_prime_UTR 20499466 20499763 20499466 ID=Msa0320230-mRNA-1:three_prime_utr;Parent=Msa0320230-mRNA-1
Gene Sequence

>Msa0320230

ATGGGTGTTTTTACTTTCAATGATGAACATGTATCAACCGTGGCTCCAGCTAAACTCTACAAAGCTCTTGCAAAAGATGCTGATGAAATCGTCCCAAAGGTGATTTCTGCTGCCCAAAGTGTTGAAATTGTTGAAGGAAATGGAGGACCAGGAACCATTAAGAAGTTATCCATGTCTGAAGATGGCAAAACCAACTTTGTGCTACACAAATTAGATGCAGTAGATGAGGCAAACTTGGGATACAACTACAGCTTAGTAGGAGGAACAGGGTTGGATGAAAGTCTAGAGAAAGTTGAATTCGAGACAAAAATTGTGGCTGGCTCTGATGGTGGATCCATTGCTAAGATTACAGTGAAATACCACACCAAAGGTGATGCAGCTCTATCTGATGCAGTCCGTGATGAAACTAAGGCCAGAGGAACTGGACTTATCAAGGCTATTGAGGGCTACGTTTTGGCAAATCCTGATTATTAA
Protein sequence

>Msa0320230

MGVFTFNDEHVSTVAPAKLYKALAKDADEIVPKVISAAQSVEIVEGNGGPGTIKKLSMSEDGKTNFVLHKLDAVDEANLGYNYSLVGGTGLDESLEKVEFETKIVAGSDGGSIAKITVKYHTKGDAALSDAVRDETKARGTGLIKAIEGYVLANPDY*