AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0528930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0528930 MtrunA17_Chr3g0087921 92.424 66 5 0 3 68 2 67 2.38e-35 114
Msa0528930 MtrunA17_Chr3g0087931 87.692 65 8 0 4 68 3 67 2.15e-32 106
Msa0528930 MtrunA17_Chr3g0087911 87.692 65 8 0 4 68 3 67 5.50e-25 87.8
Msa0528930 MtrunA17_Chr3g0088251 75.385 65 14 2 6 68 2 66 1.61e-23 84.3
Msa0528930 MtrunA17_Chr3g0087981 72.727 66 16 2 5 68 3 68 1.66e-23 84.3
Msa0528930 MtrunA17_Chr3g0088031 78.689 61 11 2 10 68 1 61 1.88e-23 84.0
Msa0528930 MtrunA17_Chr3g0088011 79.661 59 9 2 13 68 8 66 4.66e-23 82.8
Msa0528930 MtrunA17_Chr3g0088021 73.438 64 15 2 7 68 3 66 2.67e-22 81.3
Msa0528930 MtrunA17_Chr3g0087941 83.824 68 11 0 1 68 1 68 1.00e-21 79.7
Msa0528930 MtrunA17_Chr3g0088041 93.182 44 3 0 25 68 23 66 1.68e-21 79.0
Msa0528930 MtrunA17_Chr3g0088271 82.979 47 8 0 22 68 19 65 2.75e-20 75.9
Msa0528930 MtrunA17_Chr3g0088071 79.167 48 10 0 21 68 19 66 8.92e-20 74.7
Msa0528930 MtrunA17_Chr3g0088081 88.095 42 5 0 27 68 24 65 1.19e-19 74.3
Msa0528930 MtrunA17_Chr3g0088001 76.923 65 14 1 5 68 3 67 1.48e-19 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACTATGAAGTTGTTCTT+TGG 0.337246 4_1:+1914784 Msa0528930:CDS
CCAGCATCAGGACCTGGTGC+TGG 0.401353 4_1:-1914865 None:intergenic
GCAAGTTCTGTGGCTATGAT+TGG 0.405849 4_1:+1914904 Msa0528930:CDS
CCAGCACCAGGTCCTGATGC+TGG 0.415553 4_1:+1914865 Msa0528930:CDS
GATCCGGCAGCTCCAGCATC+AGG 0.425147 4_1:-1914877 None:intergenic
GGTCCTGATGCTGGAGCTGC+CGG 0.466366 4_1:+1914874 Msa0528930:CDS
CACAGAACTTGCAACAGATC+CGG 0.484083 4_1:-1914893 None:intergenic
CGTCTTCGCCGCTGTAGTCT+CGG 0.497571 4_1:+1914816 Msa0528930:CDS
CTAACGACGGACTAAGGTCT+TGG 0.503269 4_1:-1914840 None:intergenic
CTGGAGCTAACGACGGACTA+AGG 0.507562 4_1:-1914846 None:intergenic
TAACGACGGACTAAGGTCTT+GGG 0.525930 4_1:-1914839 None:intergenic
GCAGCTCCAGCATCAGGACC+TGG 0.550326 4_1:-1914871 None:intergenic
CCGTCGTTAGCTCCAGCACC+AGG 0.556130 4_1:+1914853 Msa0528930:CDS
ACCAAAGAACAACTTCATAG+TGG 0.593920 4_1:-1914785 None:intergenic
CGGATCTGTTGCAAGTTCTG+TGG 0.613339 4_1:+1914894 Msa0528930:CDS
AAAGAACAACTTCATAGTGG+TGG 0.644041 4_1:-1914782 None:intergenic
CCTGGTGCTGGAGCTAACGA+CGG 0.671877 4_1:-1914853 None:intergenic
GTCTTGGGCCGAGACTACAG+CGG 0.726320 4_1:-1914824 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGAACAACTTCATAGTGG+TGG - chr4_1:1914785-1914804 None:intergenic 35.0%
ACCACTATGAAGTTGTTCTT+TGG + chr4_1:1914784-1914803 Msa0528930:CDS 35.0%
ACCAAAGAACAACTTCATAG+TGG - chr4_1:1914788-1914807 None:intergenic 35.0%
!! TAACGACGGACTAAGGTCTT+GGG - chr4_1:1914842-1914861 None:intergenic 45.0%
CACAGAACTTGCAACAGATC+CGG - chr4_1:1914896-1914915 None:intergenic 45.0%
GCAAGTTCTGTGGCTATGAT+TGG + chr4_1:1914904-1914923 Msa0528930:CDS 45.0%
!! CTAACGACGGACTAAGGTCT+TGG - chr4_1:1914843-1914862 None:intergenic 50.0%
CGGATCTGTTGCAAGTTCTG+TGG + chr4_1:1914894-1914913 Msa0528930:CDS 50.0%
!! CTGGAGCTAACGACGGACTA+AGG - chr4_1:1914849-1914868 None:intergenic 55.0%
CGTCTTCGCCGCTGTAGTCT+CGG + chr4_1:1914816-1914835 Msa0528930:CDS 60.0%
GTCTTGGGCCGAGACTACAG+CGG - chr4_1:1914827-1914846 None:intergenic 60.0%
! CCTGGTGCTGGAGCTAACGA+CGG - chr4_1:1914856-1914875 None:intergenic 60.0%
CCGTCGTTAGCTCCAGCACC+AGG + chr4_1:1914853-1914872 Msa0528930:CDS 65.0%
CCAGCATCAGGACCTGGTGC+TGG - chr4_1:1914868-1914887 None:intergenic 65.0%
CCAGCACCAGGTCCTGATGC+TGG + chr4_1:1914865-1914884 Msa0528930:CDS 65.0%
GCAGCTCCAGCATCAGGACC+TGG - chr4_1:1914874-1914893 None:intergenic 65.0%
GGTCCTGATGCTGGAGCTGC+CGG + chr4_1:1914874-1914893 Msa0528930:CDS 65.0%
GATCCGGCAGCTCCAGCATC+AGG - chr4_1:1914880-1914899 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 1914763 1914978 1914763 ID=Msa0528930;Name=Msa0528930
chr4_1 mRNA 1914763 1914978 1914763 ID=Msa0528930-mRNA-1;Parent=Msa0528930;Name=Msa0528930-mRNA-1;_AED=0.31;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|71
chr4_1 exon 1914763 1914978 1914763 ID=Msa0528930-mRNA-1:exon:603;Parent=Msa0528930-mRNA-1
chr4_1 CDS 1914763 1914978 1914763 ID=Msa0528930-mRNA-1:cds;Parent=Msa0528930-mRNA-1
Gene Sequence

>Msa0528930

ATGGCATCATCATCATCAGCCACCACTATGAAGTTGTTCTTTGGTTTTGTCATCGTCTTCGCCGCTGTAGTCTCGGCCCAAGACCTTAGTCCGTCGTTAGCTCCAGCACCAGGTCCTGATGCTGGAGCTGCCGGATCTGTTGCAAGTTCTGTGGCTATGATTGGAGCTTCAATTGTGCTGTCAATGTTTGCTATCTTCAAGAACTTAGAAAATTAA

Protein sequence

>Msa0528930

MASSSSATTMKLFFGFVIVFAAVVSAQDLSPSLAPAPGPDAGAAGSVASSVAMIGASIVLSMFAIFKNLEN*