AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0594280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATATTATCCAATAAA+AGG 0.260189 4_2:+21625846 None:intergenic
CATCTTTGCTTCTGGCAAAA+TGG 0.262684 4_2:-21628000 Msa0594280:intron
ATTGATATGTTCTCTGCTTC+TGG 0.272947 4_2:-21626262 Msa0594280:three_prime_UTR
TACTGTAATGCAGAAGTAAA+TGG 0.338645 4_2:+21625993 None:intergenic
ACTGCACTCATCTTTGCTTC+TGG 0.346245 4_2:-21628008 Msa0594280:CDS
TTCAGATATCTCCTTTGTCT+TGG 0.406113 4_2:+21626313 None:intergenic
TTATTGGATAATATTTCAGT+GGG 0.531295 4_2:-21625842 None:intergenic
CTGAATTGATAACAAGGCAA+TGG 0.554700 4_2:-21626295 Msa0594280:exon
TTTATTGGATAATATTTCAG+TGG 0.566837 4_2:-21625843 Msa0594280:three_prime_UTR
GATTGTTCTAACAGGAGCCA+AGG 0.576547 4_2:-21626663 Msa0594280:intron
CAAAAGAGTTCGAAATTGCA+AGG 0.579809 4_2:+21626079 None:intergenic
CATTGGTAGTAAACAGACAT+TGG 0.582259 4_2:+21628624 None:intergenic
TTAGAATCCTGGTAGGAGGA+GGG 0.600102 4_2:-21627846 Msa0594280:intron
AGATATCTGAATTGATAACA+AGG 0.631988 4_2:-21626301 Msa0594280:exon
CTGTATATAAACCAAGACAA+AGG 0.691790 4_2:-21626324 Msa0594280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGCATAATAATTATTACAT+TGG + chr4_2:21625860-21625879 None:intergenic 15.0%
!! TTATTATTTACTTTGAATTC+TGG - chr4_2:21625884-21625903 Msa0594280:three_prime_UTR 15.0%
!!! ATTTATTTTGGTTTAGTAAA+AGG + chr4_2:21626819-21626838 None:intergenic 15.0%
!! ATACAAAAAATGTGTATATA+AGG + chr4_2:21627888-21627907 None:intergenic 15.0%
!! CACAAAAAAATTTCAAAAAG+TGG + chr4_2:21626212-21626231 None:intergenic 20.0%
!!! AATTTTTTTGTGTTTCTTTC+TGG - chr4_2:21626220-21626239 Msa0594280:three_prime_UTR 20.0%
!! TTGTAGTCAAATAAAAAGAT+TGG - chr4_2:21626549-21626568 Msa0594280:intron 20.0%
!!! TGTACTGTATCAATTTATTT+TGG + chr4_2:21626831-21626850 None:intergenic 20.0%
!!! GTAAATTTTGAAAAATAAGC+AGG + chr4_2:21627123-21627142 None:intergenic 20.0%
!!! AGTGTAAGCTATTTTATTTA+CGG - chr4_2:21627454-21627473 Msa0594280:intron 20.0%
!! TGTGTATAAAATTATTACCT+TGG + chr4_2:21627821-21627840 None:intergenic 20.0%
!!! TACACATTTTTTGTATTATC+AGG - chr4_2:21627892-21627911 Msa0594280:intron 20.0%
!! AGAAGAAAAGAAAAAAAAAG+TGG - chr4_2:21628297-21628316 Msa0594280:intron 20.0%
!!! AAACTGACTTAAAAATTGTA+AGG + chr4_2:21628419-21628438 None:intergenic 20.0%
!! CTGAAATATTATCCAATAAA+AGG + chr4_2:21628621-21628640 None:intergenic 20.0%
! TATTGTTTCAACAGTCAATT+TGG - chr4_2:21625988-21626007 Msa0594280:three_prime_UTR 25.0%
!! GAAAGTGAAAATTAACTTGA+TGG - chr4_2:21626175-21626194 Msa0594280:three_prime_UTR 25.0%
!! TGATTTGGAATAACAAAAGA+GGG + chr4_2:21626326-21626345 None:intergenic 25.0%
!!! TTGATTTGGAATAACAAAAG+AGG + chr4_2:21626327-21626346 None:intergenic 25.0%
!! GTATTTGCAGAATATTGATT+TGG + chr4_2:21626341-21626360 None:intergenic 25.0%
!!! TTGATTCTTTTTTAGAATCC+TGG - chr4_2:21626607-21626626 Msa0594280:intron 25.0%
! AGTTCTGCATAATAAACATA+CGG + chr4_2:21626666-21626685 None:intergenic 25.0%
! GTAAAAGGAACATTACATTA+CGG + chr4_2:21626804-21626823 None:intergenic 25.0%
!! TTATGCTGTTCTAATTTTAC+TGG - chr4_2:21626923-21626942 Msa0594280:intron 25.0%
! TTTCAGTGAAGATAATCAAA+GGG + chr4_2:21627241-21627260 None:intergenic 25.0%
! ATTTCAGTGAAGATAATCAA+AGG + chr4_2:21627242-21627261 None:intergenic 25.0%
!! ATATACTGAACTTGCTTATA+TGG - chr4_2:21627690-21627709 Msa0594280:intron 25.0%
!! TATACTGAACTTGCTTATAT+GGG - chr4_2:21627691-21627710 Msa0594280:intron 25.0%
! AAAATTAGCAAGACAATCTT+CGG + chr4_2:21627769-21627788 None:intergenic 25.0%
! TTCGATGAAACTATAACAAT+CGG + chr4_2:21628032-21628051 None:intergenic 25.0%
!!! GACGTGAATATTTTATTTTG+TGG - chr4_2:21628071-21628090 Msa0594280:intron 25.0%
!!! TTTATTTTGTGGCAGATTTT+TGG - chr4_2:21628082-21628101 Msa0594280:intron 25.0%
!! AGATATCTGAATTGATAACA+AGG - chr4_2:21628163-21628182 Msa0594280:intron 25.0%
!! TTTTGATATTGTTATGTGAC+AGG - chr4_2:21628225-21628244 Msa0594280:intron 25.0%
!!! TGAAAAAGATGATTTTGAAG+AGG - chr4_2:21628273-21628292 Msa0594280:intron 25.0%
!!! AACACTCATGATTTTAAGTT+TGG + chr4_2:21628448-21628467 None:intergenic 25.0%
GCAATGGAAAAAGAAAACAT+GGG + chr4_2:21626146-21626165 None:intergenic 30.0%
AGCAATGGAAAAAGAAAACA+TGG + chr4_2:21626147-21626166 None:intergenic 30.0%
! GTTTTCTTTTTCCATTGCTT+TGG - chr4_2:21626148-21626167 Msa0594280:three_prime_UTR 30.0%
!!! TTCTTTTTTAGAATCCTGGT+AGG - chr4_2:21626611-21626630 Msa0594280:intron 30.0%
! TTGATTTGATTCACTCTGTA+TGG - chr4_2:21626730-21626749 Msa0594280:intron 30.0%
! AAATTTTAAACTCCCAACCA+TGG + chr4_2:21626756-21626775 None:intergenic 30.0%
AATTGATACAGTACATGTGA+AGG - chr4_2:21626836-21626855 Msa0594280:intron 30.0%
! CTAATTTTACTGGTATGACT+AGG - chr4_2:21626933-21626952 Msa0594280:intron 30.0%
TGCTATTAATGTCTCTCTAA+CGG - chr4_2:21627042-21627061 Msa0594280:intron 30.0%
ATTCATCCTAATCAACTTTG+AGG + chr4_2:21627072-21627091 None:intergenic 30.0%
! ATAAACTATGCCATTTGTGT+TGG - chr4_2:21627090-21627109 Msa0594280:intron 30.0%
AAGGAATCAACTACTAATCA+AGG + chr4_2:21627328-21627347 None:intergenic 30.0%
! TGATTAGTAGTTGATTCCTT+CGG - chr4_2:21627328-21627347 Msa0594280:intron 30.0%
TTCAAGATTCAGAACATTGT+TGG - chr4_2:21627367-21627386 Msa0594280:intron 30.0%
TTGTGTTAGAATGTAGATGA+TGG - chr4_2:21627593-21627612 Msa0594280:intron 30.0%
!! TTAGAATGTAGATGATGGAA+AGG - chr4_2:21627598-21627617 Msa0594280:intron 30.0%
!! TAGAATGTAGATGATGGAAA+GGG - chr4_2:21627599-21627618 Msa0594280:intron 30.0%
!! GTCTTGCTAATTTTTGTTTC+TGG - chr4_2:21627775-21627794 Msa0594280:intron 30.0%
! TATTTTCACCCTCAAAATCA+TGG - chr4_2:21627853-21627872 Msa0594280:intron 30.0%
AGTTCAGGAAAAATCAACAA+TGG - chr4_2:21627966-21627985 Msa0594280:intron 30.0%
AGGAAAAATCAACAATGGTA+TGG - chr4_2:21627971-21627990 Msa0594280:intron 30.0%
GGAAAAATCAACAATGGTAT+GGG - chr4_2:21627972-21627991 Msa0594280:intron 30.0%
GGCAAAAAATGAATTTCTTG+CGG - chr4_2:21628103-21628122 Msa0594280:intron 30.0%
CTGTATATAAACCAAGACAA+AGG - chr4_2:21628140-21628159 Msa0594280:intron 30.0%
TACTGTAATGCAGAAGTAAA+TGG + chr4_2:21628474-21628493 None:intergenic 30.0%
! TGACTTCTATCTCCTTTTAT+TGG - chr4_2:21628606-21628625 Msa0594280:intron 30.0%
TTCTGCAAGTAAACAGACAT+TGG + chr4_2:21625967-21625986 None:intergenic 35.0%
AGTCAATTTGGACACTAAGT+TGG - chr4_2:21626000-21626019 Msa0594280:three_prime_UTR 35.0%
TAATGCAGAGTATAATCCCA+AGG - chr4_2:21626051-21626070 Msa0594280:intron 35.0%
CAAGAGATTAAACTTGCCTT+GGG + chr4_2:21626070-21626089 None:intergenic 35.0%
TCAAGAGATTAAACTTGCCT+TGG + chr4_2:21626071-21626090 None:intergenic 35.0%
!! AAGATGAGTGCAGTTGTTTT+TGG + chr4_2:21626450-21626469 None:intergenic 35.0%
!! GAAAAGCACTTATATGACAG+AGG + chr4_2:21626514-21626533 None:intergenic 35.0%
!!! TTTTTTAGAATCCTGGTAGG+AGG - chr4_2:21626614-21626633 Msa0594280:intron 35.0%
AACATTACATTACGGACATG+AGG + chr4_2:21626796-21626815 None:intergenic 35.0%
TATTAATGTCTCTCTAACGG+AGG - chr4_2:21627045-21627064 Msa0594280:intron 35.0%
! AACCAAGCTTTTGGATGATA+CGG + chr4_2:21627189-21627208 None:intergenic 35.0%
!! TAGCATTGAAACCAAGCTTT+TGG + chr4_2:21627198-21627217 None:intergenic 35.0%
AAAAGCTTGGTTTCAATGCT+AGG - chr4_2:21627197-21627216 Msa0594280:intron 35.0%
TGGTTTCAATGCTAGGTTTA+AGG - chr4_2:21627204-21627223 Msa0594280:intron 35.0%
! ATCGCTTGATGTGATTTGTA+TGG - chr4_2:21627495-21627514 Msa0594280:intron 35.0%
! TCGCTTGATGTGATTTGTAT+GGG - chr4_2:21627496-21627515 Msa0594280:intron 35.0%
GATTTGTATGGGGATAAGTA+TGG - chr4_2:21627507-21627526 Msa0594280:intron 35.0%
CAATCTTCGGTTTCTTCATA+CGG + chr4_2:21627756-21627775 None:intergenic 35.0%
TCTGGAAAGATTGTTCTAAC+AGG - chr4_2:21627793-21627812 Msa0594280:intron 35.0%
TGAGGGTGAAAATAACAGAA+GGG + chr4_2:21627847-21627866 None:intergenic 35.0%
TTGAGGGTGAAAATAACAGA+AGG + chr4_2:21627848-21627867 None:intergenic 35.0%
! CTAGTGAACCATGATTTTGA+GGG + chr4_2:21627864-21627883 None:intergenic 35.0%
TTTGTATTATCAGGTGAGAG+AGG - chr4_2:21627901-21627920 Msa0594280:intron 35.0%
ACTATAACAATCGGCATAGA+TGG + chr4_2:21628023-21628042 None:intergenic 35.0%
TCACGTCAGTAAATCAATAG+TGG + chr4_2:21628058-21628077 None:intergenic 35.0%
TTCAGATATCTCCTTTGTCT+TGG + chr4_2:21628154-21628173 None:intergenic 35.0%
! CTGAATTGATAACAAGGCAA+TGG - chr4_2:21628169-21628188 Msa0594280:intron 35.0%
ATTGATATGTTCTCTGCTTC+TGG - chr4_2:21628202-21628221 Msa0594280:intron 35.0%
CAAAAGAGTTCGAAATTGCA+AGG + chr4_2:21628388-21628407 None:intergenic 35.0%
TATACTCTGCATTACGAGCT+TGG + chr4_2:21626045-21626064 None:intergenic 40.0%
GGAAAAAGAAAACATGGGCA+AGG + chr4_2:21626141-21626160 None:intergenic 40.0%
CACTTTCACTACCAAAGCAA+TGG + chr4_2:21626162-21626181 None:intergenic 40.0%
! GTTTTGCTGCTGTCATTATG+AGG - chr4_2:21626415-21626434 Msa0594280:intron 40.0%
CATCTTTGCTTCTGGCAAAA+TGG - chr4_2:21626464-21626483 Msa0594280:intron 40.0%
AAGCACTTATATGACAGAGG+TGG + chr4_2:21626511-21626530 None:intergenic 40.0%
TGGTATGACTAGGTTTGTAC+TGG - chr4_2:21626943-21626962 Msa0594280:intron 40.0%
GAGTGAGCAACAGTCTAAAT+TGG - chr4_2:21626972-21626991 Msa0594280:intron 40.0%
AACAGTCTAAATTGGCAGCA+AGG - chr4_2:21626980-21626999 Msa0594280:intron 40.0%
! CTATGCCATTTGTGTTGGAT+TGG - chr4_2:21627095-21627114 Msa0594280:intron 40.0%
! ACCAAGCTTTTGGATGATAC+GGG + chr4_2:21627188-21627207 None:intergenic 40.0%
GTGATGTCAAGTTCCCAATA+CGG - chr4_2:21627395-21627414 Msa0594280:intron 40.0%
! CGCTTGATGTGATTTGTATG+GGG - chr4_2:21627497-21627516 Msa0594280:intron 40.0%
! CTGAACTTGCTTATATGGGA+TGG - chr4_2:21627695-21627714 Msa0594280:intron 40.0%
TTCATACGGTAGATTAACCC+AGG + chr4_2:21627742-21627761 None:intergenic 40.0%
GAGGGTGAAAATAACAGAAG+GGG + chr4_2:21627846-21627865 None:intergenic 40.0%
! CCTAGTGAACCATGATTTTG+AGG + chr4_2:21627865-21627884 None:intergenic 40.0%
CCTCAAAATCATGGTTCACT+AGG - chr4_2:21627862-21627881 Msa0594280:intron 40.0%
CACAGGGTATATTTGCTCAA+AGG + chr4_2:21627939-21627958 None:intergenic 40.0%
AAAAAGTGGCTAGAGCTAGA+TGG - chr4_2:21628311-21628330 Msa0594280:intron 40.0%
ACTGCACTCATCTTTGCTTC+TGG - chr4_2:21626456-21626475 Msa0594280:intron 45.0%
! TTTAGAATCCTGGTAGGAGG+AGG - chr4_2:21626617-21626636 Msa0594280:intron 45.0%
TTAGAATCCTGGTAGGAGGA+GGG - chr4_2:21626618-21626637 Msa0594280:intron 45.0%
TGATTCACTCTGTATGGCCA+TGG - chr4_2:21626736-21626755 Msa0594280:intron 45.0%
GTCTAAATTGGCAGCAAGGA+AGG - chr4_2:21626984-21627003 Msa0594280:intron 45.0%
! GGAGGACCTCAAAGTTGATT+AGG - chr4_2:21627063-21627082 Msa0594280:intron 45.0%
AGGCTCCAATCCAACACAAA+TGG + chr4_2:21627103-21627122 None:intergenic 45.0%
! TTCCTTCGGCTTTATGCTTC+AGG - chr4_2:21627342-21627361 Msa0594280:intron 45.0%
ATCCTGAAGCATAAAGCCGA+AGG + chr4_2:21627347-21627366 None:intergenic 45.0%
TGTCAAGTTCCCAATACGGT+TGG - chr4_2:21627399-21627418 Msa0594280:intron 45.0%
AAGTTCCCAATACGGTTGGA+GGG - chr4_2:21627403-21627422 Msa0594280:intron 45.0%
AGGGTCTTGCATATTCCCAT+GGG - chr4_2:21627422-21627441 Msa0594280:intron 45.0%
TACACTAGAGAAAGCCCCAT+GGG + chr4_2:21627440-21627459 None:intergenic 45.0%
TTACACTAGAGAAAGCCCCA+TGG + chr4_2:21627441-21627460 None:intergenic 45.0%
TCACATCAAGCGATCACGAA+GGG + chr4_2:21627489-21627508 None:intergenic 45.0%
ATCACATCAAGCGATCACGA+AGG + chr4_2:21627490-21627509 None:intergenic 45.0%
! TATGAACCTGAGCTGTTTCC+TGG - chr4_2:21627721-21627740 Msa0594280:intron 45.0%
! ATGAACCTGAGCTGTTTCCT+GGG - chr4_2:21627722-21627741 Msa0594280:intron 45.0%
ATTAACCCAGGAAACAGCTC+AGG + chr4_2:21627730-21627749 None:intergenic 45.0%
GATTGTTCTAACAGGAGCCA+AGG - chr4_2:21627801-21627820 Msa0594280:intron 45.0%
TCCTGAACTCAGTAAGCACA+GGG + chr4_2:21627955-21627974 None:intergenic 45.0%
TTCCTGAACTCAGTAAGCAC+AGG + chr4_2:21627956-21627975 None:intergenic 45.0%
TCACTCTGTATGGCCATGGT+TGG - chr4_2:21626740-21626759 Msa0594280:intron 50.0%
CACTCTGTATGGCCATGGTT+GGG - chr4_2:21626741-21626760 Msa0594280:intron 50.0%
GCCCGTATCATCCAAAAGCT+TGG - chr4_2:21627184-21627203 Msa0594280:intron 50.0%
CAAGTTCCCAATACGGTTGG+AGG - chr4_2:21627402-21627421 Msa0594280:intron 50.0%
CAAGACCCTCCAACCGTATT+GGG + chr4_2:21627411-21627430 None:intergenic 50.0%
GAGGGTCTTGCATATTCCCA+TGG - chr4_2:21627421-21627440 Msa0594280:intron 50.0%
GGGTCTTGCATATTCCCATG+GGG - chr4_2:21627423-21627442 Msa0594280:intron 50.0%
ACCCTGTGCTTACTGAGTTC+AGG - chr4_2:21627951-21627970 Msa0594280:intron 50.0%
CTAATGACCCTCCTCCTACC+AGG + chr4_2:21626628-21626647 None:intergenic 55.0%
GCAAGACCCTCCAACCGTAT+TGG + chr4_2:21627412-21627431 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 21625843 21628643 21625843 ID=Msa0594280;Name=Msa0594280
chr4_2 mRNA 21625843 21628643 21625843 ID=Msa0594280-mRNA-1;Parent=Msa0594280;Name=Msa0594280-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|0.14|0.37|0.62|0.85|0.75|8|425|60
chr4_2 exon 21625843 21626035 21625843 ID=Msa0594280-mRNA-1:exon:5999;Parent=Msa0594280-mRNA-1
chr4_2 exon 21626082 21626389 21626082 ID=Msa0594280-mRNA-1:exon:5998;Parent=Msa0594280-mRNA-1
chr4_2 exon 21626664 21626671 21626664 ID=Msa0594280-mRNA-1:exon:5997;Parent=Msa0594280-mRNA-1
chr4_2 exon 21627299 21627305 21627299 ID=Msa0594280-mRNA-1:exon:5996;Parent=Msa0594280-mRNA-1
chr4_2 exon 21627847 21627853 21627847 ID=Msa0594280-mRNA-1:exon:5995;Parent=Msa0594280-mRNA-1
chr4_2 exon 21627858 21627864 21627858 ID=Msa0594280-mRNA-1:exon:5994;Parent=Msa0594280-mRNA-1
chr4_2 exon 21628001 21628072 21628001 ID=Msa0594280-mRNA-1:exon:5993;Parent=Msa0594280-mRNA-1
chr4_2 exon 21628638 21628643 21628638 ID=Msa0594280-mRNA-1:exon:5992;Parent=Msa0594280-mRNA-1
chr4_2 CDS 21628638 21628643 21628638 ID=Msa0594280-mRNA-1:cds;Parent=Msa0594280-mRNA-1
chr4_2 CDS 21628001 21628072 21628001 ID=Msa0594280-mRNA-1:cds;Parent=Msa0594280-mRNA-1
chr4_2 CDS 21627858 21627864 21627858 ID=Msa0594280-mRNA-1:cds;Parent=Msa0594280-mRNA-1
chr4_2 CDS 21627847 21627853 21627847 ID=Msa0594280-mRNA-1:cds;Parent=Msa0594280-mRNA-1
chr4_2 CDS 21627299 21627305 21627299 ID=Msa0594280-mRNA-1:cds;Parent=Msa0594280-mRNA-1
chr4_2 CDS 21626664 21626671 21626664 ID=Msa0594280-mRNA-1:cds;Parent=Msa0594280-mRNA-1
chr4_2 CDS 21626314 21626389 21626314 ID=Msa0594280-mRNA-1:cds;Parent=Msa0594280-mRNA-1
chr4_2 three_prime_UTR 21626082 21626313 21626082 ID=Msa0594280-mRNA-1:three_prime_utr;Parent=Msa0594280-mRNA-1
chr4_2 three_prime_UTR 21625843 21626035 21625843 ID=Msa0594280-mRNA-1:three_prime_utr;Parent=Msa0594280-mRNA-1
Gene Sequence

>Msa0594280

ATGTCTCGTTTTGCTGCTGTCATTATGAGGATCAGAGAACCAAAAACAACTGCACTCATCTTTGCTTCTGGCAAAATGAATCCTGGAGGAGGTATGCCCGAGCCAAGATTTTTGGCAAAAAATGAATTTCTTGCGGAGAAGTTCGTCGAACTGTATATAAACCAAGACAAAGGAGATATCTGA

Protein sequence

>Msa0594280

MSRFAAVIMRIREPKTTALIFASGKMNPGGGMPEPRFLAKNEFLAEKFVELYINQDKGDI*