AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0021810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0021810 MtrunA17_Chr1g0173651 99.083 109 1 0 1 109 657 765 3.63e-69 221
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0021810 AT3G57630.2 67.568 111 36 0 1 111 649 759 3.07e-50 170
Msa0021810 AT3G57630.3 67.568 111 36 0 1 111 651 761 3.13e-50 170
Msa0021810 AT3G57630.1 67.568 111 36 0 1 111 651 761 3.13e-50 170
Msa0021810 AT5G11610.1 39.437 71 43 0 2 72 451 521 9.18e-12 61.2
Msa0021810 AT5G25820.1 37.681 69 43 0 2 70 559 627 1.22e-11 61.2

Find 17 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAGAATCTTTATCATGTT+TGG 0.234944 1_1:+39479995 None:intergenic
ATGCATCCCTGTGGTCATTC+AGG 0.338402 1_1:-39480425 Msa0021810:intron
CAAACATGATAAAGATTCTT+CGG 0.349930 1_1:-39479994 Msa0021810:CDS
ATGTTTGGAATTTCATCTTC+TGG 0.357658 1_1:+39480010 None:intergenic
TAGCAAATGTTCAGAAAATC+TGG 0.357752 1_1:-39479831 Msa0021810:CDS
AAACATGATAAAGATTCTTC+GGG 0.478043 1_1:-39479993 Msa0021810:intron
GCTTCAATAATCTATTGTCA+TGG 0.499832 1_1:-39477884 Msa0021810:CDS
TGTCACCACCACTTTAATAC+AGG 0.525124 1_1:-39479698 Msa0021810:intron
GATAAATACCTGTATTAAAG+TGG 0.529467 1_1:+39479690 None:intergenic
CTTCAATAATCTATTGTCAT+GGG 0.548373 1_1:-39477883 Msa0021810:CDS
AGTTCGTGGAAGTGCCATCA+AGG 0.575224 1_1:-39477859 Msa0021810:CDS
CCTATTACCTGAATGACCAC+AGG 0.579608 1_1:+39480418 None:intergenic
TTGAAGCAAATAATATGCAA+TGG 0.592942 1_1:-39477906 Msa0021810:intron
CTATTGTCATGGGAAGTTCG+TGG 0.619983 1_1:-39477873 Msa0021810:CDS
AAATACCTGTATTAAAGTGG+TGG 0.628568 1_1:+39479693 None:intergenic
CTATTACCTGAATGACCACA+GGG 0.638697 1_1:+39480419 None:intergenic
TTTACAAGGATGCATCCCTG+TGG 0.727175 1_1:-39480434 Msa0021810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTAAAGTATAAGTTAA+TGG - chr1_1:39479797-39479816 Msa0021810:CDS 10.0%
!! TAATATACTATGCAATTTAT+TGG - chr1_1:39479075-39479094 Msa0021810:intron 15.0%
!! GATAAAGAAAATAATAAAAG+AGG - chr1_1:39479479-39479498 Msa0021810:intron 15.0%
!! TCAATTAAAAACATAATCAA+GGG + chr1_1:39479544-39479563 None:intergenic 15.0%
!! TTCAATTAAAAACATAATCA+AGG + chr1_1:39479545-39479564 None:intergenic 15.0%
!!! GTAACTATTTTCTTTTTAAA+AGG + chr1_1:39479630-39479649 None:intergenic 15.0%
!! TTTAAAAAGAAAATAGTTAC+TGG - chr1_1:39479630-39479649 Msa0021810:intron 15.0%
!!! ATCTTTTAAATTATTTCCTG+GGG + chr1_1:39477919-39477938 None:intergenic 20.0%
!!! CATCTTTTAAATTATTTCCT+GGG + chr1_1:39477920-39477939 None:intergenic 20.0%
!!! TGAATTTTTAAAATTGACAG+AGG - chr1_1:39478571-39478590 Msa0021810:intron 20.0%
!!! GAAAAGCATAATTTGTTAAA+AGG + chr1_1:39478988-39479007 None:intergenic 20.0%
!! AAAAAAATCATGTATATCAC+TGG + chr1_1:39479303-39479322 None:intergenic 20.0%
!!! ATATGACCACAAATATATTT+TGG + chr1_1:39479781-39479800 None:intergenic 20.0%
!! AACTTGATTTAAAAGATAAG+TGG + chr1_1:39480358-39480377 None:intergenic 20.0%
!! CCATCTTTTAAATTATTTCC+TGG + chr1_1:39477921-39477940 None:intergenic 25.0%
! CCAGGAAATAATTTAAAAGA+TGG - chr1_1:39477918-39477937 Msa0021810:intron 25.0%
! TAAACTGAAATCATCAATGA+TGG - chr1_1:39478162-39478181 Msa0021810:intron 25.0%
! CAAACATGATAAAGATTCTT+CGG - chr1_1:39478302-39478321 Msa0021810:intron 25.0%
! AAACATGATAAAGATTCTTC+GGG - chr1_1:39478303-39478322 Msa0021810:intron 25.0%
! CATACAATAACTATATGCAA+AGG + chr1_1:39478395-39478414 None:intergenic 25.0%
! GCATATAGTTATTGTATGAA+TGG - chr1_1:39478397-39478416 Msa0021810:intron 25.0%
! ATAGTTATTGTATGAATGGT+TGG - chr1_1:39478401-39478420 Msa0021810:intron 25.0%
! GATAAATACCTGTATTAAAG+TGG + chr1_1:39478609-39478628 None:intergenic 25.0%
!!! TTCTAGAATAGTTTTGACAA+AGG + chr1_1:39478634-39478653 None:intergenic 25.0%
!!! CAAAACTATTCTAGAATCAA+TGG - chr1_1:39478638-39478657 Msa0021810:intron 25.0%
! CTTGTTGAATACAAAAACTA+TGG + chr1_1:39479127-39479146 None:intergenic 25.0%
!! CATAGTTTTTGTATTCAACA+AGG - chr1_1:39479125-39479144 Msa0021810:intron 25.0%
!! TGGAGATAGTATCATATTTT+AGG + chr1_1:39479262-39479281 None:intergenic 25.0%
!!! CTAGTTATTTTACAATTCCA+CGG + chr1_1:39479387-39479406 None:intergenic 25.0%
! AAGTTCGAAAACAATGAATT+TGG - chr1_1:39479563-39479582 Msa0021810:intron 25.0%
!!! TTTTCAAAATTAGTTTCCAG+AGG - chr1_1:39479588-39479607 Msa0021810:intron 25.0%
!! TTTCAAAATTAGTTTCCAGA+GGG - chr1_1:39479589-39479608 Msa0021810:intron 25.0%
! CTCATGAAATTGAATGAAAA+TGG + chr1_1:39480070-39480089 None:intergenic 25.0%
! AACATGACATTAAGAACAAA+TGG + chr1_1:39480234-39480253 None:intergenic 25.0%
! GAACAAATGAATGAAACAAA+TGG + chr1_1:39480304-39480323 None:intergenic 25.0%
! TTGAAGCAAATAATATGCAA+TGG - chr1_1:39480390-39480409 Msa0021810:intron 25.0%
! CTTCAATAATCTATTGTCAT+GGG - chr1_1:39480413-39480432 Msa0021810:intron 25.0%
TTATTTCCTGGGGAAAAAAA+GGG + chr1_1:39477909-39477928 None:intergenic 30.0%
ATTATTTCCTGGGGAAAAAA+AGG + chr1_1:39477910-39477929 None:intergenic 30.0%
TAAAAACGATTCATGAGCAT+TGG - chr1_1:39478075-39478094 Msa0021810:intron 30.0%
! TAGTTGAGTACATTTTCGTA+TGG + chr1_1:39478244-39478263 None:intergenic 30.0%
! ATGTTTGGAATTTCATCTTC+TGG + chr1_1:39478289-39478308 None:intergenic 30.0%
CGAAGAATCTTTATCATGTT+TGG + chr1_1:39478304-39478323 None:intergenic 30.0%
ACTATGCAACGCATAAAAAA+GGG + chr1_1:39478346-39478365 None:intergenic 30.0%
AACTATGCAACGCATAAAAA+AGG + chr1_1:39478347-39478366 None:intergenic 30.0%
TAGCAAATGTTCAGAAAATC+TGG - chr1_1:39478465-39478484 Msa0021810:intron 30.0%
!!! TTTTGGACATGTTGATGATT+GGG - chr1_1:39478544-39478563 Msa0021810:intron 30.0%
AAATACCTGTATTAAAGTGG+TGG + chr1_1:39478606-39478625 None:intergenic 30.0%
! CATACACACATTTTATGTGA+GGG + chr1_1:39479326-39479345 None:intergenic 30.0%
! ACATACACACATTTTATGTG+AGG + chr1_1:39479327-39479346 None:intergenic 30.0%
AAATAACTAGTGTATGTGCA+CGG - chr1_1:39479397-39479416 Msa0021810:intron 30.0%
ATCAAGGGAACAATTCAAAT+AGG + chr1_1:39479529-39479548 None:intergenic 30.0%
GAGAGGTAACATTAGAAAAA+CGG - chr1_1:39479739-39479758 Msa0021810:CDS 30.0%
CATTGGCCAAAATATATTTG+TGG - chr1_1:39479772-39479791 Msa0021810:CDS 30.0%
AAAGTGAGAATTCAAAGCAA+TGG - chr1_1:39479844-39479863 Msa0021810:CDS 30.0%
ATGAAAATGGTCAAATGTTG+AGG + chr1_1:39480057-39480076 None:intergenic 30.0%
! TAACTTGAAGAAAGCACAAA+TGG + chr1_1:39480260-39480279 None:intergenic 30.0%
AACAAATGGAAAACATGACA+TGG + chr1_1:39480290-39480309 None:intergenic 30.0%
GCTTCAATAATCTATTGTCA+TGG - chr1_1:39480412-39480431 Msa0021810:intron 30.0%
! GATTCATCTTCAGAGTTTTG+TGG - chr1_1:39478031-39478050 Msa0021810:intron 35.0%
CATTGGTATAGTGATGAAAG+AGG - chr1_1:39478092-39478111 Msa0021810:intron 35.0%
!!! GTTTTGTTTCCTTGTTCTGT+AGG - chr1_1:39478204-39478223 Msa0021810:intron 35.0%
TATGCAACGCATAAAAAAGG+GGG + chr1_1:39478344-39478363 None:intergenic 35.0%
CTATGCAACGCATAAAAAAG+GGG + chr1_1:39478345-39478364 None:intergenic 35.0%
ATAACTATATGCAAAGGCTC+TGG + chr1_1:39478389-39478408 None:intergenic 35.0%
! CTATTTTGCTTGAAGCTGAA+AGG - chr1_1:39478510-39478529 Msa0021810:intron 35.0%
!!! CTTTTGGACATGTTGATGAT+TGG - chr1_1:39478543-39478562 Msa0021810:intron 35.0%
CTCTATATGTCTACAAGTTC+TGG + chr1_1:39478676-39478695 None:intergenic 35.0%
!!! TTCTCTTTTTTCAGCCTCTA+GGG + chr1_1:39478743-39478762 None:intergenic 35.0%
!!! TTTCTCTTTTTTCAGCCTCT+AGG + chr1_1:39478744-39478763 None:intergenic 35.0%
TTGATTCTTCTCAGCCAATT+TGG - chr1_1:39478894-39478913 Msa0021810:intron 35.0%
TGATTCTTCTCAGCCAATTT+GGG - chr1_1:39478895-39478914 Msa0021810:intron 35.0%
! GCTTTTCAAAAGCTCCATTA+TGG - chr1_1:39479001-39479020 Msa0021810:intron 35.0%
TTTCAAAAGCTCCATTATGG+TGG - chr1_1:39479004-39479023 Msa0021810:intron 35.0%
ACAAGTTACATAATGACCCT+TGG - chr1_1:39479157-39479176 Msa0021810:intron 35.0%
AAAACGAATAATTCACCCTC+TGG + chr1_1:39479607-39479626 None:intergenic 35.0%
AGGTAACATTAGAAAAACGG+AGG - chr1_1:39479742-39479761 Msa0021810:CDS 35.0%
GGTAACATTAGAAAAACGGA+GGG - chr1_1:39479743-39479762 Msa0021810:CDS 35.0%
AAATGGTCAAATGTTGAGGT+GGG + chr1_1:39480053-39480072 None:intergenic 35.0%
AAAATGGTCAAATGTTGAGG+TGG + chr1_1:39480054-39480073 None:intergenic 35.0%
! TATACCATGCATTCTAGCTA+AGG - chr1_1:39480147-39480166 Msa0021810:intron 35.0%
TTAACCTTAGCTAGAATGCA+TGG + chr1_1:39480154-39480173 None:intergenic 35.0%
TCTCCTCTATTTGAGTAATC+TGG - chr1_1:39480185-39480204 Msa0021810:intron 35.0%
AGACCAGATTACTCAAATAG+AGG + chr1_1:39480191-39480210 None:intergenic 35.0%
AAGATAAGTGGATACACATG+AGG + chr1_1:39480346-39480365 None:intergenic 35.0%
CTATTACCTGAATGACCACA+GGG + chr1_1:39477880-39477899 None:intergenic 40.0%
TGTTTCCTTGTTCTGTAGGA+TGG - chr1_1:39478208-39478227 Msa0021810:intron 40.0%
GTTTCCTTGTTCTGTAGGAT+GGG - chr1_1:39478209-39478228 Msa0021810:intron 40.0%
AAATCCCATCCTACAGAACA+AGG + chr1_1:39478216-39478235 None:intergenic 40.0%
ATGCAACGCATAAAAAAGGG+GGG + chr1_1:39478343-39478362 None:intergenic 40.0%
!! ATGAATGGTTGGTGATATGC+AGG - chr1_1:39478412-39478431 Msa0021810:intron 40.0%
!! TGAATGGTTGGTGATATGCA+GGG - chr1_1:39478413-39478432 Msa0021810:intron 40.0%
!! GAAAGGCAAAAAGCTGCTTT+TGG - chr1_1:39478527-39478546 Msa0021810:intron 40.0%
TGTCACCACCACTTTAATAC+AGG - chr1_1:39478598-39478617 Msa0021810:intron 40.0%
TAGAAACAACTCAACCCACT+TGG - chr1_1:39478812-39478831 Msa0021810:intron 40.0%
AGAAACAACTCAACCCACTT+GGG - chr1_1:39478813-39478832 Msa0021810:intron 40.0%
!! GTAGTATTGGCTTGAGACTT+GGG - chr1_1:39478844-39478863 Msa0021810:intron 40.0%
CTAAACTAGTCCACCCAAAT+TGG + chr1_1:39478911-39478930 None:intergenic 40.0%
ATGCTTCTGTTCCACCATAA+TGG + chr1_1:39479018-39479037 None:intergenic 40.0%
AGTTACATAATGACCCTTGG+AGG - chr1_1:39479160-39479179 Msa0021810:intron 40.0%
ACCCTTGGAGGAAACAAATT+GGG - chr1_1:39479172-39479191 Msa0021810:intron 40.0%
ATTGGGCACAACAAGAAGTT+TGG - chr1_1:39479189-39479208 Msa0021810:intron 40.0%
TATGATACTATCTCCACTGC+AGG - chr1_1:39479266-39479285 Msa0021810:intron 40.0%
GTATCTTGAGTCTGTCTCAT+AGG - chr1_1:39479353-39479372 Msa0021810:intron 40.0%
GTCTTCTAATCTGTTGAGTG+TGG - chr1_1:39479717-39479736 Msa0021810:CDS 40.0%
AGCTAGAATGCATGGTATAG+AGG + chr1_1:39480146-39480165 None:intergenic 40.0%
TTTACAAGGATGCATCCCTG+TGG - chr1_1:39477862-39477881 Msa0021810:CDS 45.0%
CCTATTACCTGAATGACCAC+AGG + chr1_1:39477881-39477900 None:intergenic 45.0%
CCTGTGGTCATTCAGGTAAT+AGG - chr1_1:39477878-39477897 Msa0021810:CDS 45.0%
TGCAACGCATAAAAAAGGGG+GGG + chr1_1:39478342-39478361 None:intergenic 45.0%
!! GAATGGTTGGTGATATGCAG+GGG - chr1_1:39478414-39478433 Msa0021810:intron 45.0%
ATTGAATTCTCCCTCCCTAG+AGG - chr1_1:39478726-39478745 Msa0021810:intron 45.0%
!!! CTTTTTTCAGCCTCTAGGGA+GGG + chr1_1:39478739-39478758 None:intergenic 45.0%
!!! TCTTTTTTCAGCCTCTAGGG+AGG + chr1_1:39478740-39478759 None:intergenic 45.0%
!! TTGGGTTGACCTGGTAGTAT+TGG - chr1_1:39478831-39478850 Msa0021810:intron 45.0%
AGTCTCAAGCCAATACTACC+AGG + chr1_1:39478843-39478862 None:intergenic 45.0%
!! GGTAGTATTGGCTTGAGACT+TGG - chr1_1:39478843-39478862 Msa0021810:intron 45.0%
GAATCAAACCTGAGACCTTG+AGG + chr1_1:39478881-39478900 None:intergenic 45.0%
TTCTTCTCAGCCAATTTGGG+TGG - chr1_1:39478898-39478917 Msa0021810:intron 45.0%
GACCCTTGGAGGAAACAAAT+TGG - chr1_1:39479171-39479190 Msa0021810:intron 45.0%
! GCCCAATTTGTTTCCTCCAA+GGG + chr1_1:39479176-39479195 None:intergenic 45.0%
! TGCCCAATTTGTTTCCTCCA+AGG + chr1_1:39479177-39479196 None:intergenic 45.0%
TGGATTACCTAACCAGTGTG+TGG - chr1_1:39479209-39479228 Msa0021810:intron 45.0%
! GTGTTAACCACACACTGGTT+AGG + chr1_1:39479219-39479238 None:intergenic 45.0%
! AGATGGTGTTAACCACACAC+TGG + chr1_1:39479224-39479243 None:intergenic 45.0%
TGTGTGGTTAACACCATCTG+AGG - chr1_1:39479225-39479244 Msa0021810:intron 45.0%
TGGTTAACACCATCTGAGGA+AGG - chr1_1:39479229-39479248 Msa0021810:intron 45.0%
GGTTGAATGATTTCCTGCAG+TGG + chr1_1:39479282-39479301 None:intergenic 45.0%
CTAATCTGTTGAGTGTGGAG+AGG - chr1_1:39479722-39479741 Msa0021810:CDS 45.0%
AAAACGGAGGGAAGTAGCAT+TGG - chr1_1:39479755-39479774 Msa0021810:CDS 45.0%
! GCTTTGAATTCTCACGTTCG+AGG + chr1_1:39480031-39480050 None:intergenic 45.0%
CAAGAATCTGCTTGTGTGAC+AGG + chr1_1:39480111-39480130 None:intergenic 45.0%
CTATTGTCATGGGAAGTTCG+TGG - chr1_1:39480423-39480442 Msa0021810:intron 45.0%
ATGCATCCCTGTGGTCATTC+AGG - chr1_1:39477871-39477890 Msa0021810:CDS 50.0%
!!! GTTGTGCCCTTTTTTTCCCC+AGG - chr1_1:39477900-39477919 Msa0021810:CDS 50.0%
CAACGCATAAAAAAGGGGGG+GGG + chr1_1:39478340-39478359 None:intergenic 50.0%
GCAACGCATAAAAAAGGGGG+GGG + chr1_1:39478341-39478360 None:intergenic 50.0%
ACTACCAGGTCAACCCAAGT+GGG + chr1_1:39478829-39478848 None:intergenic 50.0%
TACTACCAGGTCAACCCAAG+TGG + chr1_1:39478830-39478849 None:intergenic 50.0%
TCAAACCTGAGACCTTGAGG+AGG + chr1_1:39478878-39478897 None:intergenic 50.0%
GGAACTTGACCTTCCTCAGA+TGG + chr1_1:39479241-39479260 None:intergenic 50.0%
!! AGTTCGTGGAAGTGCCATCA+AGG - chr1_1:39480437-39480456 Msa0021810:CDS 50.0%
!! TCAACCCACTTGGGTTGACC+TGG - chr1_1:39478822-39478841 Msa0021810:intron 55.0%
TCTCATAGGCTGTCACACCG+TGG - chr1_1:39479367-39479386 Msa0021810:intron 55.0%
! TGGGAGTGTGCTCCTCCTCA+AGG - chr1_1:39478863-39478882 Msa0021810:intron 60.0%
GTGCTCCTCCTCAAGGTCTC+AGG - chr1_1:39478870-39478889 Msa0021810:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 39477851 39480467 39477851 ID=Msa0021810;Name=Msa0021810
chr1_1 mRNA 39477851 39480467 39477851 ID=Msa0021810-mRNA-1;Parent=Msa0021810;Name=Msa0021810-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|0.66|0.75|0.75|0.33|0.5|4|0|132
chr1_1 exon 39477851 39477922 39477851 ID=Msa0021810-mRNA-1:exon:12075;Parent=Msa0021810-mRNA-1
chr1_1 exon 39479699 39479884 39479699 ID=Msa0021810-mRNA-1:exon:12074;Parent=Msa0021810-mRNA-1
chr1_1 exon 39479994 39480092 39479994 ID=Msa0021810-mRNA-1:exon:12073;Parent=Msa0021810-mRNA-1
chr1_1 exon 39480426 39480467 39480426 ID=Msa0021810-mRNA-1:exon:12072;Parent=Msa0021810-mRNA-1
chr1_1 CDS 39480426 39480467 39480426 ID=Msa0021810-mRNA-1:cds;Parent=Msa0021810-mRNA-1
chr1_1 CDS 39479994 39480092 39479994 ID=Msa0021810-mRNA-1:cds;Parent=Msa0021810-mRNA-1
chr1_1 CDS 39479699 39479884 39479699 ID=Msa0021810-mRNA-1:cds;Parent=Msa0021810-mRNA-1
chr1_1 CDS 39477851 39477922 39477851 ID=Msa0021810-mRNA-1:cds;Parent=Msa0021810-mRNA-1
Gene Sequence

>Msa0021810

GAAGACAGTGTTTTACAAGGATGCATCCCTGTGGTCATTCAGGATGGGATTTTATTACCATACGAAAATGTACTCAACTATGATAGCTTTGCTGTTCGAATACCAGAAGATGAAATTCCAAACATGATAAAGATTCTTCGGGGGTTCAATGAAACAGAAATCAATTTGAAGTTAGCAAATGTTCAGAAAATCTGGCAAAGATTCTTGTATCGTGATTCTATTTTGCTTGAAGCTGAAAGGCAAAAAGCTGCTTTTGGACATGTTGATGATTGGGCAGTTGAATTTTTAAAATTGACAGAGGATGATGTCACCACCACTTTAATACAGCAAATAATATGCAATGGCTTCAATAATCTATTGTCATGGGAAGTTCGTGGAAGTGCCATCAAGGATAGCTGA

Protein sequence

>Msa0021810

EDSVLQGCIPVVIQDGILLPYENVLNYDSFAVRIPEDEIPNMIKILRGFNETEINLKLANVQKIWQRFLYRDSILLEAERQKAAFGHVDDWAVEFLKLTEDDVTTTLIQQIICNGFNNLLSWEVRGSAIKDS*