Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0780820 | sp|Q9C635|HSFB4_ARATH | 57.727 | 220 | 50 | 6 | 20 | 200 | 29 | 244 | 4.56e-75 | 234 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0780820 | G7KCV7 | 94.488 | 254 | 3 | 1 | 1 | 243 | 1 | 254 | 5.79e-176 | 493 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0780820 | TF | HSF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0018920 | Msa0780820 | 0.808040 | 3.765380e-50 | -8.615850e-47 |
| Msa0050530 | Msa0780820 | 0.802205 | 6.264741e-49 | -8.615850e-47 |
| Msa0079410 | Msa0780820 | 0.810634 | 1.045711e-50 | -8.615850e-47 |
| Msa0100600 | Msa0780820 | 0.834700 | 2.575239e-56 | -8.615850e-47 |
| Msa0100890 | Msa0780820 | 0.808655 | 2.784294e-50 | -8.615850e-47 |
| Msa0109730 | Msa0780820 | 0.800352 | 1.500491e-48 | -8.615850e-47 |
| Msa0605520 | Msa0780820 | 0.807693 | 4.461840e-50 | -8.615850e-47 |
| Msa0607660 | Msa0780820 | 0.851716 | 7.405790e-61 | -8.615850e-47 |
| Msa0640800 | Msa0780820 | 0.825453 | 4.649855e-54 | -8.615850e-47 |
| Msa0654740 | Msa0780820 | 0.809386 | 1.941184e-50 | -8.615850e-47 |
| Msa0660260 | Msa0780820 | 0.823263 | 1.522154e-53 | -8.615850e-47 |
| Msa0688620 | Msa0780820 | 0.839711 | 1.345334e-57 | -8.615850e-47 |
| Msa0690570 | Msa0780820 | 0.881421 | 2.434544e-70 | -8.615850e-47 |
| Msa0707260 | Msa0780820 | 0.825452 | 4.652117e-54 | -8.615850e-47 |
| Msa0717900 | Msa0780820 | 0.801884 | 7.293191e-49 | -8.615850e-47 |
| Msa0720280 | Msa0780820 | 0.835796 | 1.362093e-56 | -8.615850e-47 |
| Msa0749190 | Msa0780820 | 0.836509 | 8.975599e-57 | -8.615850e-47 |
| Msa0750520 | Msa0780820 | 0.839000 | 2.058463e-57 | -8.615850e-47 |
| Msa0751720 | Msa0780820 | 0.846845 | 1.681261e-59 | -8.615850e-47 |
| Msa0780820 | Msa0786740 | 0.809245 | 2.081328e-50 | -8.615850e-47 |
| Msa0780820 | Msa0803140 | 0.833209 | 6.083618e-56 | -8.615850e-47 |
| Msa0780820 | Msa0816230 | 0.810227 | 1.279948e-50 | -8.615850e-47 |
| Msa0780820 | Msa0821260 | 0.843501 | 1.348186e-58 | -8.615850e-47 |
| Msa0780820 | Msa0821350 | 0.825165 | 5.440041e-54 | -8.615850e-47 |
| Msa0780820 | Msa0822510 | 0.828907 | 6.925426e-55 | -8.615850e-47 |
| Msa0780820 | Msa0827420 | 0.835272 | 1.848056e-56 | -8.615850e-47 |
| Msa0780820 | Msa0831820 | 0.830669 | 2.578725e-55 | -8.615850e-47 |
| Msa0780820 | Msa0841500 | 0.811064 | 8.439264e-51 | -8.615850e-47 |
| Msa0780820 | Msa0966710 | 0.819141 | 1.357096e-52 | -8.615850e-47 |
| Msa0780820 | Msa1000970 | 0.827049 | 1.938559e-54 | -8.615850e-47 |
| Msa0780820 | Msa1008260 | 0.809963 | 1.459370e-50 | -8.615850e-47 |
| Msa0780820 | Msa1027160 | 0.801356 | 9.360476e-49 | -8.615850e-47 |
| Msa0780820 | Msa1071410 | 0.840012 | 1.123326e-57 | -8.615850e-47 |
| Msa0780820 | Msa1076020 | 0.806201 | 9.227368e-50 | -8.615850e-47 |
| Msa0780820 | Msa1154630 | 0.817731 | 2.832298e-52 | -8.615850e-47 |
| Msa0780820 | Msa1163180 | 0.817086 | 3.956624e-52 | -8.615850e-47 |
| Msa0780820 | Msa1219770 | 0.818896 | 1.542828e-52 | -8.615850e-47 |
| Msa0780820 | Msa1277110 | 0.811871 | 5.637031e-51 | -8.615850e-47 |
| Msa0780820 | Msa1294790 | 0.820684 | 6.021813e-53 | -8.615850e-47 |
| Msa0780820 | Msa1315500 | 0.819617 | 1.057249e-52 | -8.615850e-47 |
| Msa0780820 | Msa1333360 | 0.801999 | 6.908879e-49 | -8.615850e-47 |
| Msa0780820 | Msa1339230 | 0.801288 | 9.664890e-49 | -8.615850e-47 |
| Msa0780820 | Msa1372030 | 0.843041 | 1.787939e-58 | -8.615850e-47 |
| Msa0780820 | Msa1381800 | 0.836199 | 1.076491e-56 | -8.615850e-47 |
| Msa0780820 | Msa1389820 | 0.820220 | 7.696935e-53 | -8.615850e-47 |
| Msa0780820 | Msa1390210 | 0.808415 | 3.132692e-50 | -8.615850e-47 |
| Msa0780820 | Msa1393560 | 0.815554 | 8.710277e-52 | -8.615850e-47 |
| Msa0780820 | Msa1420830 | 0.800967 | 1.124215e-48 | -8.615850e-47 |
| Msa0780820 | Msa1426320 | 0.800846 | 1.190070e-48 | -8.615850e-47 |
| Msa0125590 | Msa0780820 | 0.811116 | 8.223296e-51 | -8.615850e-47 |
| Msa0168380 | Msa0780820 | 0.825167 | 5.433306e-54 | -8.615850e-47 |
| Msa0170070 | Msa0780820 | 0.806794 | 6.918663e-50 | -8.615850e-47 |
| Msa0172590 | Msa0780820 | 0.843218 | 1.604116e-58 | -8.615850e-47 |
| Msa0195440 | Msa0780820 | 0.813337 | 2.693424e-51 | -8.615850e-47 |
| Msa0204970 | Msa0780820 | 0.813736 | 2.200894e-51 | -8.615850e-47 |
| Msa0364680 | Msa0780820 | 0.801262 | 9.781768e-49 | -8.615850e-47 |
| Msa0373170 | Msa0780820 | 0.825243 | 5.214199e-54 | -8.615850e-47 |
| Msa0382230 | Msa0780820 | 0.802646 | 5.082291e-49 | -8.615850e-47 |
| Msa0388250 | Msa0780820 | 0.806123 | 9.583117e-50 | -8.615850e-47 |
| Msa0415820 | Msa0780820 | 0.805623 | 1.220571e-49 | -8.615850e-47 |
| Msa0416210 | Msa0780820 | 0.814733 | 1.325298e-51 | -8.615850e-47 |
| Msa0421750 | Msa0780820 | 0.806015 | 1.010015e-49 | -8.615850e-47 |
| Msa0433630 | Msa0780820 | 0.837280 | 5.704408e-57 | -8.615850e-47 |
| Msa0467690 | Msa0780820 | 0.822409 | 2.406458e-53 | -8.615850e-47 |
| Msa0477480 | Msa0780820 | 0.806807 | 6.874287e-50 | -8.615850e-47 |
| Msa0281540 | Msa0780820 | 0.825550 | 4.408886e-54 | -8.615850e-47 |
| Msa0288400 | Msa0780820 | 0.847530 | 1.090802e-59 | -8.615850e-47 |
| Msa0320580 | Msa0780820 | 0.815517 | 8.876684e-52 | -8.615850e-47 |
| Msa0342220 | Msa0780820 | 0.847405 | 1.181121e-59 | -8.615850e-47 |
| Msa0344110 | Msa0780820 | 0.817740 | 2.819359e-52 | -8.615850e-47 |
| Msa0506880 | Msa0780820 | 0.815940 | 7.145796e-52 | -8.615850e-47 |
| Msa0507810 | Msa0780820 | 0.806971 | 6.347806e-50 | -8.615850e-47 |
| Msa0507820 | Msa0780820 | 0.811882 | 5.604637e-51 | -8.615850e-47 |
| Msa0507920 | Msa0780820 | 0.802520 | 5.395660e-49 | -8.615850e-47 |
| Msa0514020 | Msa0780820 | 0.817715 | 2.855727e-52 | -8.615850e-47 |
| Msa0520680 | Msa0780820 | 0.802773 | 4.784743e-49 | -8.615850e-47 |
| Msa0521510 | Msa0780820 | 0.852514 | 4.391023e-61 | -8.615850e-47 |
| Msa0521530 | Msa0780820 | 0.816895 | 4.368350e-52 | -8.615850e-47 |
| Msa0544410 | Msa0780820 | 0.833904 | 4.079499e-56 | -8.615850e-47 |
| Msa0544430 | Msa0780820 | 0.809764 | 1.610296e-50 | -8.615850e-47 |
| Msa0554300 | Msa0780820 | 0.802831 | 4.654448e-49 | -8.615850e-47 |
| Msa0571350 | Msa0780820 | 0.826412 | 2.751912e-54 | -8.615850e-47 |
| Msa0572830 | Msa0780820 | 0.844897 | 5.686620e-59 | -8.615850e-47 |
| Msa0582460 | Msa0780820 | 0.814666 | 1.371528e-51 | -8.615850e-47 |
| Msa0586200 | Msa0780820 | 0.800319 | 1.523700e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0780820 | MtrunA17_Chr5g0441531 | 94.488 | 254 | 3 | 1 | 1 | 243 | 1 | 254 | 1.11e-179 | 493 |
| Msa0780820 | MtrunA17_Chr3g0134031 | 45.882 | 340 | 79 | 13 | 1 | 239 | 1 | 336 | 6.32e-81 | 247 |
| Msa0780820 | MtrunA17_Chr4g0004151 | 96.386 | 83 | 3 | 0 | 161 | 243 | 1 | 83 | 2.20e-53 | 167 |
| Msa0780820 | MtrunA17_Chr5g0397881 | 40.088 | 227 | 98 | 4 | 15 | 204 | 14 | 239 | 2.28e-46 | 157 |
| Msa0780820 | MtrunA17_Chr7g0257331 | 41.791 | 201 | 96 | 3 | 18 | 207 | 15 | 205 | 2.48e-44 | 149 |
| Msa0780820 | MtrunA17_Chr1g0203601 | 37.097 | 248 | 120 | 6 | 17 | 235 | 10 | 250 | 1.03e-43 | 149 |
| Msa0780820 | MtrunA17_Chr6g0472421 | 36.226 | 265 | 125 | 7 | 16 | 238 | 24 | 286 | 1.18e-40 | 142 |
| Msa0780820 | MtrunA17_Chr5g0402291 | 35.510 | 245 | 117 | 7 | 18 | 231 | 2 | 236 | 3.95e-38 | 134 |
| Msa0780820 | MtrunA17_Chr8g0375041 | 35.458 | 251 | 115 | 7 | 18 | 243 | 2 | 230 | 1.11e-37 | 132 |
| Msa0780820 | MtrunA17_Chr5g0410881 | 42.408 | 191 | 87 | 3 | 22 | 201 | 29 | 207 | 2.62e-35 | 131 |
| Msa0780820 | MtrunA17_Chr6g0467041 | 62.366 | 93 | 24 | 1 | 24 | 105 | 21 | 113 | 8.78e-34 | 124 |
| Msa0780820 | MtrunA17_Chr7g0260331 | 59.574 | 94 | 27 | 1 | 23 | 105 | 47 | 140 | 2.73e-31 | 118 |
| Msa0780820 | MtrunA17_Chr8g0379701 | 60.606 | 99 | 27 | 2 | 18 | 105 | 6 | 103 | 7.92e-31 | 117 |
| Msa0780820 | MtrunA17_Chr1g0185131 | 40.936 | 171 | 59 | 4 | 23 | 181 | 30 | 170 | 3.41e-30 | 115 |
| Msa0780820 | MtrunA17_Chr1g0204741 | 44.654 | 159 | 57 | 3 | 23 | 170 | 38 | 176 | 3.69e-30 | 114 |
| Msa0780820 | MtrunA17_Chr8g0378061 | 61.538 | 91 | 24 | 1 | 26 | 105 | 23 | 113 | 4.49e-30 | 116 |
| Msa0780820 | MtrunA17_Chr2g0330821 | 53.922 | 102 | 36 | 1 | 15 | 105 | 69 | 170 | 2.64e-27 | 107 |
| Msa0780820 | MtrunA17_Chr4g0071051 | 38.922 | 167 | 82 | 4 | 23 | 169 | 99 | 265 | 1.06e-26 | 106 |
| Msa0780820 | MtrunA17_Chr4g0047421 | 36.145 | 166 | 83 | 4 | 22 | 172 | 30 | 187 | 1.44e-26 | 102 |
| Msa0780820 | MtrunA17_Chr2g0284481 | 52.000 | 100 | 36 | 2 | 18 | 106 | 9 | 107 | 4.04e-26 | 104 |
| Msa0780820 | MtrunA17_Chr3g0135831 | 37.107 | 159 | 70 | 2 | 23 | 170 | 44 | 183 | 1.20e-25 | 103 |
| Msa0780820 | MtrunA17_Chr4g0039891 | 38.854 | 157 | 72 | 3 | 25 | 170 | 12 | 155 | 2.18e-25 | 103 |
| Msa0780820 | MtrunA17_Chr6g0485571 | 51.020 | 98 | 37 | 1 | 19 | 105 | 5 | 102 | 2.29e-25 | 102 |
| Msa0780820 | MtrunA17_Chr8g0392001 | 53.125 | 96 | 33 | 2 | 24 | 107 | 21 | 116 | 1.12e-23 | 97.8 |
| Msa0780820 | MtrunA17_Chr1g0179321 | 65.672 | 67 | 23 | 0 | 19 | 85 | 43 | 109 | 2.20e-23 | 90.9 |
| Msa0780820 | MtrunA17_Chr4g0023771 | 60.938 | 64 | 24 | 1 | 25 | 87 | 15 | 78 | 8.26e-20 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0780820 | AT1G46264.1 | 57.727 | 220 | 50 | 6 | 20 | 200 | 29 | 244 | 4.64e-76 | 234 |
| Msa0780820 | AT4G36990.1 | 36.508 | 252 | 129 | 5 | 12 | 236 | 1 | 248 | 2.37e-44 | 150 |
| Msa0780820 | AT5G62020.1 | 35.827 | 254 | 133 | 4 | 18 | 242 | 16 | 268 | 1.65e-41 | 144 |
| Msa0780820 | AT4G11660.1 | 63.107 | 103 | 27 | 1 | 17 | 108 | 51 | 153 | 2.50e-39 | 140 |
| Msa0780820 | AT1G32330.1 | 41.848 | 184 | 84 | 4 | 22 | 193 | 34 | 206 | 4.10e-35 | 130 |
| Msa0780820 | AT1G32330.2 | 41.848 | 184 | 84 | 4 | 22 | 193 | 34 | 206 | 4.13e-35 | 130 |
| Msa0780820 | AT2G41690.1 | 39.227 | 181 | 89 | 4 | 23 | 183 | 38 | 217 | 1.68e-34 | 124 |
| Msa0780820 | AT4G17750.1 | 57.009 | 107 | 35 | 1 | 15 | 110 | 42 | 148 | 1.18e-33 | 127 |
| Msa0780820 | AT3G22830.1 | 58.163 | 98 | 30 | 1 | 20 | 106 | 56 | 153 | 2.35e-30 | 117 |
| Msa0780820 | AT3G02990.1 | 58.333 | 96 | 28 | 2 | 21 | 105 | 20 | 114 | 1.65e-29 | 115 |
| Msa0780820 | AT5G16820.1 | 59.000 | 100 | 28 | 3 | 21 | 109 | 24 | 121 | 1.78e-29 | 115 |
| Msa0780820 | AT5G16820.2 | 59.000 | 100 | 28 | 3 | 21 | 109 | 24 | 121 | 1.78e-29 | 115 |
| Msa0780820 | AT5G43840.1 | 54.286 | 105 | 37 | 1 | 23 | 116 | 17 | 121 | 3.17e-29 | 111 |
| Msa0780820 | AT5G03720.2 | 57.143 | 98 | 31 | 1 | 25 | 111 | 55 | 152 | 3.33e-29 | 114 |
| Msa0780820 | AT5G03720.1 | 57.143 | 98 | 31 | 1 | 25 | 111 | 55 | 152 | 3.33e-29 | 114 |
| Msa0780820 | AT2G26150.1 | 52.577 | 97 | 35 | 1 | 20 | 105 | 39 | 135 | 1.81e-27 | 108 |
| Msa0780820 | AT2G26150.4 | 52.577 | 97 | 35 | 1 | 20 | 105 | 39 | 135 | 1.81e-27 | 108 |
| Msa0780820 | AT3G63350.1 | 33.028 | 218 | 114 | 6 | 19 | 210 | 22 | 233 | 4.48e-27 | 105 |
| Msa0780820 | AT4G13980.1 | 58.242 | 91 | 27 | 1 | 26 | 105 | 24 | 114 | 7.20e-27 | 108 |
| Msa0780820 | AT3G51910.1 | 53.125 | 96 | 34 | 1 | 20 | 104 | 24 | 119 | 1.07e-25 | 102 |
| Msa0780820 | AT3G24520.1 | 51.579 | 95 | 35 | 1 | 22 | 105 | 14 | 108 | 3.61e-25 | 101 |
| Msa0780820 | AT5G54070.1 | 48.421 | 95 | 38 | 1 | 21 | 104 | 67 | 161 | 8.15e-25 | 100 |
| Msa0780820 | AT4G18880.1 | 60.606 | 99 | 27 | 2 | 18 | 105 | 9 | 106 | 1.24e-23 | 98.6 |
| Msa0780820 | AT1G67970.1 | 42.857 | 119 | 56 | 2 | 24 | 130 | 18 | 136 | 4.39e-23 | 96.7 |
| Msa0780820 | AT5G45710.3 | 38.953 | 172 | 86 | 4 | 25 | 180 | 13 | 181 | 2.22e-22 | 94.4 |
| Msa0780820 | AT5G45710.1 | 38.953 | 172 | 86 | 4 | 25 | 180 | 13 | 181 | 2.22e-22 | 94.4 |
| Msa0780820 | AT4G18870.1 | 37.647 | 85 | 42 | 1 | 25 | 98 | 146 | 230 | 5.31e-12 | 64.7 |
| Msa0780820 | AT4G18870.1 | 35.135 | 111 | 59 | 4 | 26 | 126 | 14 | 121 | 7.37e-12 | 64.3 |
Find 38 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATATCAGTGTTTGATTT+AGG | 0.203557 | 5_2:-62911967 | None:intergenic |
| AGCTCTTCTGTGAAGCTATT+TGG | 0.233498 | 5_2:+62912216 | Msa0780820:CDS |
| AAAAGTAGCACATAAGTCTT+TGG | 0.298184 | 5_2:+62910715 | Msa0780820:five_prime_UTR |
| CTATTTGGTGTTCCTCTTTG+TGG | 0.339191 | 5_2:+62912231 | Msa0780820:CDS |
| CTATTACAATCTTTATCATT+TGG | 0.339266 | 5_2:-62912195 | None:intergenic |
| ATGAAACTACATTTGTTGTT+TGG | 0.362655 | 5_2:+62910889 | Msa0780820:CDS |
| AGAAGATCTCTAGCAAATTC+AGG | 0.373696 | 5_2:-62910918 | None:intergenic |
| TCTTTATCATTTGGTAATTG+AGG | 0.392241 | 5_2:-62912186 | None:intergenic |
| CTTTGTTCAAAGGGTGAAGC+AGG | 0.399996 | 5_2:-62912111 | None:intergenic |
| ATTGTTGCTTCTTTGTTCAA+AGG | 0.403141 | 5_2:-62912121 | None:intergenic |
| TCTTATTAATCAAGATTACT+AGG | 0.404553 | 5_2:-62912270 | None:intergenic |
| ACAACTTCTCAAGCTTTGTT+AGG | 0.420026 | 5_2:+62910961 | Msa0780820:CDS |
| ATCCAGCATATACTTTCATC+TGG | 0.441694 | 5_2:-62911925 | None:intergenic |
| TTGTTGCTTCTTTGTTCAAA+GGG | 0.445911 | 5_2:-62912120 | None:intergenic |
| AAACTACATTTGTTGTTTGG+AGG | 0.447885 | 5_2:+62910892 | Msa0780820:CDS |
| TGGTATGTCTTTGTCAAGAA+AGG | 0.453363 | 5_2:-62910819 | None:intergenic |
| ATTGTTGTTGATAGTGTTGA+GGG | 0.457477 | 5_2:-62911873 | None:intergenic |
| AGATAACATGGTGTTCACTA+TGG | 0.459097 | 5_2:+62910773 | Msa0780820:CDS |
| TTTGATTTAGGAGATGGTAA+TGG | 0.467405 | 5_2:-62911955 | None:intergenic |
| TGAGAGGTTATATTAAACTT+AGG | 0.479796 | 5_2:+62910639 | None:intergenic |
| AAGTCTTTGGTTTAGTGTGA+TGG | 0.479864 | 5_2:+62910728 | Msa0780820:exon |
| TCAGTGTTTGATTTAGGAGA+TGG | 0.490616 | 5_2:-62911961 | None:intergenic |
| TCACTGACCACATTGTATCT+TGG | 0.491347 | 5_2:+62910859 | Msa0780820:CDS |
| GTCTTTGTCAAGAAAGGAGC+AGG | 0.498324 | 5_2:-62910813 | None:intergenic |
| CTGAGGACAATCAAAGACTA+AGG | 0.512782 | 5_2:+62912001 | Msa0780820:CDS |
| AACCAGATGAAAGTATATGC+TGG | 0.518742 | 5_2:+62911923 | Msa0780820:CDS |
| ATCTATTATGTGAAATCCAC+AGG | 0.520532 | 5_2:+62911842 | Msa0780820:CDS |
| TATCTTAACAGCACTTTCTG+AGG | 0.558486 | 5_2:+62911984 | Msa0780820:CDS |
| TATTGTTGTTGATAGTGTTG+AGG | 0.584009 | 5_2:-62911874 | None:intergenic |
| TGAGAGGATCATCAACAAGT+TGG | 0.584975 | 5_2:-62910839 | None:intergenic |
| GAGGTTATATTAAACTTAGG+TGG | 0.604711 | 5_2:+62910642 | Msa0780820:five_prime_UTR |
| GATACAATGTGGTCAGTGAG+AGG | 0.630246 | 5_2:-62910855 | None:intergenic |
| TGATGGCTTTCACATTAGAG+AGG | 0.643157 | 5_2:+62910745 | Msa0780820:exon |
| AGAGAGGTGTGAAGATAACA+TGG | 0.644284 | 5_2:+62910761 | Msa0780820:CDS |
| TTGTTGTTGATAGTGTTGAG+GGG | 0.651120 | 5_2:-62911872 | None:intergenic |
| AGATCTCTAGCAAATTCAGG+AGG | 0.698266 | 5_2:-62910915 | None:intergenic |
| AGGGTGAAGCAGGACTCACA+TGG | 0.700311 | 5_2:-62912101 | None:intergenic |
| CATCACTCCAAGATACAATG+TGG | 0.717706 | 5_2:-62910866 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTAGAAATTATATGAAAA+TGG | - | chr5_2:62911234-62911253 | None:intergenic | 15.0% |
| !! | GAAAAAAATTAAAGTTGTAT+TGG | + | chr5_2:62911652-62911671 | Msa0780820:intron | 15.0% |
| !!! | CATTTTGAAACAAAAATTTT+TGG | + | chr5_2:62911692-62911711 | Msa0780820:intron | 15.0% |
| !! | GTTTAGAAATTATATGAAAA+TGG | - | chr5_2:62911234-62911253 | None:intergenic | 15.0% |
| !! | GAAAAAAATTAAAGTTGTAT+TGG | + | chr5_2:62911652-62911671 | Msa0780820:intron | 15.0% |
| !!! | CATTTTGAAACAAAAATTTT+TGG | + | chr5_2:62911692-62911711 | Msa0780820:intron | 15.0% |
| !! | TAACTATAATAATGAACTGA+AGG | - | chr5_2:62911067-62911086 | None:intergenic | 20.0% |
| !! | TTATGAAAATATGCTGAAAA+CGG | + | chr5_2:62911280-62911299 | Msa0780820:intron | 20.0% |
| !! | AAATGGAATGAAATAATCAT+TGG | + | chr5_2:62911525-62911544 | Msa0780820:intron | 20.0% |
| !!! | TGTCTTTTTCTAATAAAGTA+AGG | - | chr5_2:62911574-62911593 | None:intergenic | 20.0% |
| !!! | TATTTTGAAGCTTATTGAAT+TGG | + | chr5_2:62912152-62912171 | Msa0780820:CDS | 20.0% |
| !! | CTATTACAATCTTTATCATT+TGG | - | chr5_2:62912198-62912217 | None:intergenic | 20.0% |
| !! | TAACTATAATAATGAACTGA+AGG | - | chr5_2:62911067-62911086 | None:intergenic | 20.0% |
| !! | TTATGAAAATATGCTGAAAA+CGG | + | chr5_2:62911280-62911299 | Msa0780820:intron | 20.0% |
| !! | AAATGGAATGAAATAATCAT+TGG | + | chr5_2:62911525-62911544 | Msa0780820:intron | 20.0% |
| !!! | TGTCTTTTTCTAATAAAGTA+AGG | - | chr5_2:62911574-62911593 | None:intergenic | 20.0% |
| !!! | TATTTTGAAGCTTATTGAAT+TGG | + | chr5_2:62912152-62912171 | Msa0780820:CDS | 20.0% |
| !! | CTATTACAATCTTTATCATT+TGG | - | chr5_2:62912198-62912217 | None:intergenic | 20.0% |
| !!! | ATGAAACTACATTTGTTGTT+TGG | + | chr5_2:62910889-62910908 | Msa0780820:CDS | 25.0% |
| ! | TTGTTGTGCTTGAAAAAATT+GGG | - | chr5_2:62910945-62910964 | None:intergenic | 25.0% |
| ! | GTACTAAAACAATATCAAGA+AGG | - | chr5_2:62911041-62911060 | None:intergenic | 25.0% |
| ! | TATAATAATGAACTGAAGGA+TGG | - | chr5_2:62911063-62911082 | None:intergenic | 25.0% |
| !!! | ATAAGCTATTTCTGTAACAA+AGG | + | chr5_2:62911192-62911211 | Msa0780820:intron | 25.0% |
| !! | CATATTTTCATAAGTTCTCT+AGG | - | chr5_2:62911273-62911292 | None:intergenic | 25.0% |
| ! | CTGACAATAATTTGAGAAAA+TGG | + | chr5_2:62911367-62911386 | Msa0780820:intron | 25.0% |
| ! | GCAAATGATTACTTCAAAAA+AGG | + | chr5_2:62911811-62911830 | Msa0780820:CDS | 25.0% |
| !! | AAGATATCAGTGTTTGATTT+AGG | - | chr5_2:62911970-62911989 | None:intergenic | 25.0% |
| !! | TCTTTATCATTTGGTAATTG+AGG | - | chr5_2:62912189-62912208 | None:intergenic | 25.0% |
| !!! | ATGAAACTACATTTGTTGTT+TGG | + | chr5_2:62910889-62910908 | Msa0780820:CDS | 25.0% |
| ! | TTGTTGTGCTTGAAAAAATT+GGG | - | chr5_2:62910945-62910964 | None:intergenic | 25.0% |
| ! | GTACTAAAACAATATCAAGA+AGG | - | chr5_2:62911041-62911060 | None:intergenic | 25.0% |
| ! | TATAATAATGAACTGAAGGA+TGG | - | chr5_2:62911063-62911082 | None:intergenic | 25.0% |
| !!! | ATAAGCTATTTCTGTAACAA+AGG | + | chr5_2:62911192-62911211 | Msa0780820:intron | 25.0% |
| !! | CATATTTTCATAAGTTCTCT+AGG | - | chr5_2:62911273-62911292 | None:intergenic | 25.0% |
| ! | CTGACAATAATTTGAGAAAA+TGG | + | chr5_2:62911367-62911386 | Msa0780820:intron | 25.0% |
| ! | GCAAATGATTACTTCAAAAA+AGG | + | chr5_2:62911811-62911830 | Msa0780820:CDS | 25.0% |
| !! | AAGATATCAGTGTTTGATTT+AGG | - | chr5_2:62911970-62911989 | None:intergenic | 25.0% |
| !! | TCTTTATCATTTGGTAATTG+AGG | - | chr5_2:62912189-62912208 | None:intergenic | 25.0% |
| GAGGTTATATTAAACTTAGG+TGG | + | chr5_2:62910642-62910661 | Msa0780820:five_prime_UTR | 30.0% | |
| AAAAGTAGCACATAAGTCTT+TGG | + | chr5_2:62910715-62910734 | Msa0780820:five_prime_UTR | 30.0% | |
| !! | AAACTACATTTGTTGTTTGG+AGG | + | chr5_2:62910892-62910911 | Msa0780820:CDS | 30.0% |
| GTTGTTGTGCTTGAAAAAAT+TGG | - | chr5_2:62910946-62910965 | None:intergenic | 30.0% | |
| AATTTGAGAAAATGGAACAG+CGG | + | chr5_2:62911375-62911394 | Msa0780820:intron | 30.0% | |
| TTTGCACACAGATTAAGAAA+TGG | + | chr5_2:62911508-62911527 | Msa0780820:intron | 30.0% | |
| !! | AAGTGACACTCATTTTGAAA+CGG | + | chr5_2:62911718-62911737 | Msa0780820:intron | 30.0% |
| ATCTATTATGTGAAATCCAC+AGG | + | chr5_2:62911842-62911861 | Msa0780820:CDS | 30.0% | |
| !! | ATTGTTGTTGATAGTGTTGA+GGG | - | chr5_2:62911876-62911895 | None:intergenic | 30.0% |
| !! | TATTGTTGTTGATAGTGTTG+AGG | - | chr5_2:62911877-62911896 | None:intergenic | 30.0% |
| TCATCTGGTTGAAAAATTTG+TGG | - | chr5_2:62911913-62911932 | None:intergenic | 30.0% | |
| ! | TTTGATTTAGGAGATGGTAA+TGG | - | chr5_2:62911958-62911977 | None:intergenic | 30.0% |
| ! | TTGTTGCTTCTTTGTTCAAA+GGG | - | chr5_2:62912123-62912142 | None:intergenic | 30.0% |
| ! | ATTGTTGCTTCTTTGTTCAA+AGG | - | chr5_2:62912124-62912143 | None:intergenic | 30.0% |
| GAGGTTATATTAAACTTAGG+TGG | + | chr5_2:62910642-62910661 | Msa0780820:five_prime_UTR | 30.0% | |
| AAAAGTAGCACATAAGTCTT+TGG | + | chr5_2:62910715-62910734 | Msa0780820:five_prime_UTR | 30.0% | |
| !! | AAACTACATTTGTTGTTTGG+AGG | + | chr5_2:62910892-62910911 | Msa0780820:CDS | 30.0% |
| GTTGTTGTGCTTGAAAAAAT+TGG | - | chr5_2:62910946-62910965 | None:intergenic | 30.0% | |
| AATTTGAGAAAATGGAACAG+CGG | + | chr5_2:62911375-62911394 | Msa0780820:intron | 30.0% | |
| TTTGCACACAGATTAAGAAA+TGG | + | chr5_2:62911508-62911527 | Msa0780820:intron | 30.0% | |
| !! | AAGTGACACTCATTTTGAAA+CGG | + | chr5_2:62911718-62911737 | Msa0780820:intron | 30.0% |
| ATCTATTATGTGAAATCCAC+AGG | + | chr5_2:62911842-62911861 | Msa0780820:CDS | 30.0% | |
| !! | ATTGTTGTTGATAGTGTTGA+GGG | - | chr5_2:62911876-62911895 | None:intergenic | 30.0% |
| !! | TATTGTTGTTGATAGTGTTG+AGG | - | chr5_2:62911877-62911896 | None:intergenic | 30.0% |
| TCATCTGGTTGAAAAATTTG+TGG | - | chr5_2:62911913-62911932 | None:intergenic | 30.0% | |
| ! | TTTGATTTAGGAGATGGTAA+TGG | - | chr5_2:62911958-62911977 | None:intergenic | 30.0% |
| ! | TTGTTGCTTCTTTGTTCAAA+GGG | - | chr5_2:62912123-62912142 | None:intergenic | 30.0% |
| ! | ATTGTTGCTTCTTTGTTCAA+AGG | - | chr5_2:62912124-62912143 | None:intergenic | 30.0% |
| ! | AAGTCTTTGGTTTAGTGTGA+TGG | + | chr5_2:62910728-62910747 | Msa0780820:exon | 35.0% |
| AGATAACATGGTGTTCACTA+TGG | + | chr5_2:62910773-62910792 | Msa0780820:CDS | 35.0% | |
| TGGTATGTCTTTGTCAAGAA+AGG | - | chr5_2:62910822-62910841 | None:intergenic | 35.0% | |
| AGAAGATCTCTAGCAAATTC+AGG | - | chr5_2:62910921-62910940 | None:intergenic | 35.0% | |
| !! | ACAACTTCTCAAGCTTTGTT+AGG | + | chr5_2:62910961-62910980 | Msa0780820:CDS | 35.0% |
| CACATGTATCAATGTCATGT+TGG | + | chr5_2:62911406-62911425 | Msa0780820:intron | 35.0% | |
| !! | AAGCGACACTCATTTTGAAA+CGG | - | chr5_2:62911483-62911502 | None:intergenic | 35.0% |
| !! | TTCAAAATGAGTGTCGCTTT+AGG | + | chr5_2:62911484-62911503 | Msa0780820:intron | 35.0% |
| AAAGTTTGAGAGGGTAAAGT+TGG | + | chr5_2:62911627-62911646 | Msa0780820:intron | 35.0% | |
| !! | TTGTTGTTGATAGTGTTGAG+GGG | - | chr5_2:62911875-62911894 | None:intergenic | 35.0% |
| ! | AACCAGATGAAAGTATATGC+TGG | + | chr5_2:62911923-62911942 | Msa0780820:CDS | 35.0% |
| ATCCAGCATATACTTTCATC+TGG | - | chr5_2:62911928-62911947 | None:intergenic | 35.0% | |
| ! | TCAGTGTTTGATTTAGGAGA+TGG | - | chr5_2:62911964-62911983 | None:intergenic | 35.0% |
| TATCTTAACAGCACTTTCTG+AGG | + | chr5_2:62911984-62912003 | Msa0780820:CDS | 35.0% | |
| TTCCTCTTTGTGGAAAAAAG+AGG | + | chr5_2:62912241-62912260 | Msa0780820:CDS | 35.0% | |
| TCCTCTTTGTGGAAAAAAGA+GGG | + | chr5_2:62912242-62912261 | Msa0780820:CDS | 35.0% | |
| ! | AAGTCTTTGGTTTAGTGTGA+TGG | + | chr5_2:62910728-62910747 | Msa0780820:exon | 35.0% |
| AGATAACATGGTGTTCACTA+TGG | + | chr5_2:62910773-62910792 | Msa0780820:CDS | 35.0% | |
| TGGTATGTCTTTGTCAAGAA+AGG | - | chr5_2:62910822-62910841 | None:intergenic | 35.0% | |
| AGAAGATCTCTAGCAAATTC+AGG | - | chr5_2:62910921-62910940 | None:intergenic | 35.0% | |
| !! | ACAACTTCTCAAGCTTTGTT+AGG | + | chr5_2:62910961-62910980 | Msa0780820:CDS | 35.0% |
| CACATGTATCAATGTCATGT+TGG | + | chr5_2:62911406-62911425 | Msa0780820:intron | 35.0% | |
| !! | AAGCGACACTCATTTTGAAA+CGG | - | chr5_2:62911483-62911502 | None:intergenic | 35.0% |
| !! | TTCAAAATGAGTGTCGCTTT+AGG | + | chr5_2:62911484-62911503 | Msa0780820:intron | 35.0% |
| AAAGTTTGAGAGGGTAAAGT+TGG | + | chr5_2:62911627-62911646 | Msa0780820:intron | 35.0% | |
| !! | TTGTTGTTGATAGTGTTGAG+GGG | - | chr5_2:62911875-62911894 | None:intergenic | 35.0% |
| ! | AACCAGATGAAAGTATATGC+TGG | + | chr5_2:62911923-62911942 | Msa0780820:CDS | 35.0% |
| ATCCAGCATATACTTTCATC+TGG | - | chr5_2:62911928-62911947 | None:intergenic | 35.0% | |
| ! | TCAGTGTTTGATTTAGGAGA+TGG | - | chr5_2:62911964-62911983 | None:intergenic | 35.0% |
| TATCTTAACAGCACTTTCTG+AGG | + | chr5_2:62911984-62912003 | Msa0780820:CDS | 35.0% | |
| TTCCTCTTTGTGGAAAAAAG+AGG | + | chr5_2:62912241-62912260 | Msa0780820:CDS | 35.0% | |
| TCCTCTTTGTGGAAAAAAGA+GGG | + | chr5_2:62912242-62912261 | Msa0780820:CDS | 35.0% | |
| TGATGGCTTTCACATTAGAG+AGG | + | chr5_2:62910745-62910764 | Msa0780820:exon | 40.0% | |
| ! | AGAGAGGTGTGAAGATAACA+TGG | + | chr5_2:62910761-62910780 | Msa0780820:CDS | 40.0% |
| TGAGAGGATCATCAACAAGT+TGG | - | chr5_2:62910842-62910861 | None:intergenic | 40.0% | |
| TCACTGACCACATTGTATCT+TGG | + | chr5_2:62910859-62910878 | Msa0780820:CDS | 40.0% | |
| CATCACTCCAAGATACAATG+TGG | - | chr5_2:62910869-62910888 | None:intergenic | 40.0% | |
| AGATCTCTAGCAAATTCAGG+AGG | - | chr5_2:62910918-62910937 | None:intergenic | 40.0% | |
| !! | GCTGAAAACGGCTTTTGAAA+AGG | + | chr5_2:62911292-62911311 | Msa0780820:intron | 40.0% |
| !!! | GACACTCATTTTGAAACGGA+GGG | - | chr5_2:62911722-62911741 | None:intergenic | 40.0% |
| TGCGAGAGAAAAGTTTGAGA+GGG | + | chr5_2:62911618-62911637 | Msa0780820:intron | 40.0% | |
| !!! | TGACACTCATTTTGAAACGG+AGG | + | chr5_2:62911721-62911740 | Msa0780820:intron | 40.0% |
| !!! | GACACTCATTTTGAAACGGA+GGG | + | chr5_2:62911722-62911741 | Msa0780820:intron | 40.0% |
| GTACTTCTCTTCTTCTTGCA+GGG | + | chr5_2:62911754-62911773 | Msa0780820:intron | 40.0% | |
| TCAAGAAGGTTGTTGCTGAT+AGG | + | chr5_2:62911779-62911798 | Msa0780820:intron | 40.0% | |
| CTGAGGACAATCAAAGACTA+AGG | + | chr5_2:62912001-62912020 | Msa0780820:CDS | 40.0% | |
| AGCTCTTCTGTGAAGCTATT+TGG | + | chr5_2:62912216-62912235 | Msa0780820:CDS | 40.0% | |
| !! | CTATTTGGTGTTCCTCTTTG+TGG | + | chr5_2:62912231-62912250 | Msa0780820:CDS | 40.0% |
| !!! | ACCCTCTTTTTTCCACAAAG+AGG | - | chr5_2:62912246-62912265 | None:intergenic | 40.0% |
| TGATGGCTTTCACATTAGAG+AGG | + | chr5_2:62910745-62910764 | Msa0780820:exon | 40.0% | |
| ! | AGAGAGGTGTGAAGATAACA+TGG | + | chr5_2:62910761-62910780 | Msa0780820:CDS | 40.0% |
| TGAGAGGATCATCAACAAGT+TGG | - | chr5_2:62910842-62910861 | None:intergenic | 40.0% | |
| TCACTGACCACATTGTATCT+TGG | + | chr5_2:62910859-62910878 | Msa0780820:CDS | 40.0% | |
| CATCACTCCAAGATACAATG+TGG | - | chr5_2:62910869-62910888 | None:intergenic | 40.0% | |
| AGATCTCTAGCAAATTCAGG+AGG | - | chr5_2:62910918-62910937 | None:intergenic | 40.0% | |
| !! | GCTGAAAACGGCTTTTGAAA+AGG | + | chr5_2:62911292-62911311 | Msa0780820:intron | 40.0% |
| !!! | GACACTCATTTTGAAACGGA+GGG | - | chr5_2:62911722-62911741 | None:intergenic | 40.0% |
| TGCGAGAGAAAAGTTTGAGA+GGG | + | chr5_2:62911618-62911637 | Msa0780820:intron | 40.0% | |
| !!! | TGACACTCATTTTGAAACGG+AGG | + | chr5_2:62911721-62911740 | Msa0780820:intron | 40.0% |
| !!! | GACACTCATTTTGAAACGGA+GGG | + | chr5_2:62911722-62911741 | Msa0780820:intron | 40.0% |
| GTACTTCTCTTCTTCTTGCA+GGG | + | chr5_2:62911754-62911773 | Msa0780820:intron | 40.0% | |
| TCAAGAAGGTTGTTGCTGAT+AGG | + | chr5_2:62911779-62911798 | Msa0780820:intron | 40.0% | |
| CTGAGGACAATCAAAGACTA+AGG | + | chr5_2:62912001-62912020 | Msa0780820:CDS | 40.0% | |
| AGCTCTTCTGTGAAGCTATT+TGG | + | chr5_2:62912216-62912235 | Msa0780820:CDS | 40.0% | |
| !! | CTATTTGGTGTTCCTCTTTG+TGG | + | chr5_2:62912231-62912250 | Msa0780820:CDS | 40.0% |
| !!! | ACCCTCTTTTTTCCACAAAG+AGG | - | chr5_2:62912246-62912265 | None:intergenic | 40.0% |
| GTCTTTGTCAAGAAAGGAGC+AGG | - | chr5_2:62910816-62910835 | None:intergenic | 45.0% | |
| GATACAATGTGGTCAGTGAG+AGG | - | chr5_2:62910858-62910877 | None:intergenic | 45.0% | |
| !!! | CGACACTCATTTTGAAACGG+AGG | - | chr5_2:62911480-62911499 | None:intergenic | 45.0% |
| GTGCGAGAGAAAAGTTTGAG+AGG | + | chr5_2:62911617-62911636 | Msa0780820:intron | 45.0% | |
| CGTACTTCTCTTCTTCTTGC+AGG | + | chr5_2:62911753-62911772 | Msa0780820:intron | 45.0% | |
| CTTCTTGCAGGGTTTCAAGA+AGG | + | chr5_2:62911765-62911784 | Msa0780820:intron | 45.0% | |
| AGAAGGTTGTTGCTGATAGG+TGG | + | chr5_2:62911782-62911801 | Msa0780820:intron | 45.0% | |
| GAAGGTTGTTGCTGATAGGT+GGG | + | chr5_2:62911783-62911802 | Msa0780820:intron | 45.0% | |
| CTTTGTTCAAAGGGTGAAGC+AGG | - | chr5_2:62912114-62912133 | None:intergenic | 45.0% | |
| GTCTTTGTCAAGAAAGGAGC+AGG | - | chr5_2:62910816-62910835 | None:intergenic | 45.0% | |
| GATACAATGTGGTCAGTGAG+AGG | - | chr5_2:62910858-62910877 | None:intergenic | 45.0% | |
| !!! | CGACACTCATTTTGAAACGG+AGG | - | chr5_2:62911480-62911499 | None:intergenic | 45.0% |
| GTGCGAGAGAAAAGTTTGAG+AGG | + | chr5_2:62911617-62911636 | Msa0780820:intron | 45.0% | |
| CGTACTTCTCTTCTTCTTGC+AGG | + | chr5_2:62911753-62911772 | Msa0780820:intron | 45.0% | |
| CTTCTTGCAGGGTTTCAAGA+AGG | + | chr5_2:62911765-62911784 | Msa0780820:intron | 45.0% | |
| AGAAGGTTGTTGCTGATAGG+TGG | + | chr5_2:62911782-62911801 | Msa0780820:intron | 45.0% | |
| GAAGGTTGTTGCTGATAGGT+GGG | + | chr5_2:62911783-62911802 | Msa0780820:intron | 45.0% | |
| CTTTGTTCAAAGGGTGAAGC+AGG | - | chr5_2:62912114-62912133 | None:intergenic | 45.0% | |
| ! | GTTGAGGGGTTTTTCTCCTG+TGG | - | chr5_2:62911861-62911880 | None:intergenic | 50.0% |
| ! | GTTGAGGGGTTTTTCTCCTG+TGG | - | chr5_2:62911861-62911880 | None:intergenic | 50.0% |
| AGGGTGAAGCAGGACTCACA+TGG | - | chr5_2:62912104-62912123 | None:intergenic | 55.0% | |
| AGGGTGAAGCAGGACTCACA+TGG | - | chr5_2:62912104-62912123 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 62910640 | 62912287 | 62910640 | ID=Msa0780820;Name=Msa0780820 |
| chr5_2 | mRNA | 62910640 | 62912287 | 62910640 | ID=Msa0780820-mRNA-1;Parent=Msa0780820;Name=Msa0780820-mRNA-1;_AED=0.08;_eAED=0.08;_QI=107|0|0.5|1|1|1|2|0|243 |
| chr5_2 | exon | 62910640 | 62910998 | 62910640 | ID=Msa0780820-mRNA-1:exon:18663;Parent=Msa0780820-mRNA-1 |
| chr5_2 | exon | 62911808 | 62912287 | 62911808 | ID=Msa0780820-mRNA-1:exon:18664;Parent=Msa0780820-mRNA-1 |
| chr5_2 | five_prime_UTR | 62910640 | 62910746 | 62910640 | ID=Msa0780820-mRNA-1:five_prime_utr;Parent=Msa0780820-mRNA-1 |
| chr5_2 | CDS | 62910747 | 62910998 | 62910747 | ID=Msa0780820-mRNA-1:cds;Parent=Msa0780820-mRNA-1 |
| chr5_2 | CDS | 62911808 | 62912287 | 62911808 | ID=Msa0780820-mRNA-1:cds;Parent=Msa0780820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
