Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821350 | XP_003617219.1 | 92.520 | 254 | 1 | 1 | 1 | 236 | 1 | 254 | 1.54e-169 | 477 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821350 | sp|Q9C635|HSFB4_ARATH | 54.222 | 225 | 52 | 7 | 16 | 193 | 24 | 244 | 2.00e-68 | 217 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821350 | G7KCV7 | 92.520 | 254 | 1 | 1 | 1 | 236 | 1 | 254 | 7.34e-170 | 477 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0821350 | TF | HSF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0018920 | Msa0821350 | 0.811148 | 8.093339e-51 | -8.615850e-47 |
| Msa0030070 | Msa0821350 | 0.817938 | 2.542790e-52 | -8.615850e-47 |
| Msa0091350 | Msa0821350 | 0.807474 | 4.965309e-50 | -8.615850e-47 |
| Msa0100600 | Msa0821350 | 0.818771 | 1.647012e-52 | -8.615850e-47 |
| Msa0608350 | Msa0821350 | 0.815496 | 8.974734e-52 | -8.615850e-47 |
| Msa0615630 | Msa0821350 | 0.804339 | 2.265567e-49 | -8.615850e-47 |
| Msa0620500 | Msa0821350 | 0.822625 | 2.143211e-53 | -8.615850e-47 |
| Msa0640800 | Msa0821350 | 0.814176 | 1.760149e-51 | -8.615850e-47 |
| Msa0647440 | Msa0821350 | 0.813024 | 3.154943e-51 | -8.615850e-47 |
| Msa0648760 | Msa0821350 | 0.821041 | 4.985000e-53 | -8.615850e-47 |
| Msa0654740 | Msa0821350 | 0.813610 | 2.345888e-51 | -8.615850e-47 |
| Msa0657210 | Msa0821350 | 0.800189 | 1.619641e-48 | -8.615850e-47 |
| Msa0659200 | Msa0821350 | 0.810211 | 1.290158e-50 | -8.615850e-47 |
| Msa0688620 | Msa0821350 | 0.841224 | 5.408913e-58 | -8.615850e-47 |
| Msa0690320 | Msa0821350 | 0.805900 | 1.067860e-49 | -8.615850e-47 |
| Msa0691270 | Msa0821350 | 0.821939 | 3.092613e-53 | -8.615850e-47 |
| Msa0701250 | Msa0821350 | 0.819170 | 1.336974e-52 | -8.615850e-47 |
| Msa0705180 | Msa0821350 | 0.803182 | 3.938872e-49 | -8.615850e-47 |
| Msa0707260 | Msa0821350 | 0.828097 | 1.086559e-54 | -8.615850e-47 |
| Msa0717900 | Msa0821350 | 0.826941 | 2.057732e-54 | -8.615850e-47 |
| Msa0718040 | Msa0821350 | 0.817909 | 2.582209e-52 | -8.615850e-47 |
| Msa0720280 | Msa0821350 | 0.842286 | 2.836311e-58 | -8.615850e-47 |
| Msa0749190 | Msa0821350 | 0.838672 | 2.501996e-57 | -8.615850e-47 |
| Msa0750520 | Msa0821350 | 0.838219 | 3.274078e-57 | -8.615850e-47 |
| Msa0780820 | Msa0821350 | 0.825165 | 5.440041e-54 | -8.615850e-47 |
| Msa0803140 | Msa0821350 | 0.840488 | 8.434869e-58 | -8.615850e-47 |
| Msa0816080 | Msa0821350 | 0.823366 | 1.440188e-53 | -8.615850e-47 |
| Msa0821260 | Msa0821350 | 0.924233 | 9.254621e-90 | -8.615850e-47 |
| Msa0821350 | Msa0831820 | 0.808301 | 3.313021e-50 | -8.615850e-47 |
| Msa0821350 | Msa0837680 | 0.835650 | 1.483238e-56 | -8.615850e-47 |
| Msa0821350 | Msa0841500 | 0.836810 | 7.522764e-57 | -8.615850e-47 |
| Msa0821350 | Msa0861440 | 0.821831 | 3.275780e-53 | -8.615850e-47 |
| Msa0821350 | Msa0876630 | 0.801654 | 8.129961e-49 | -8.615850e-47 |
| Msa0821350 | Msa0900090 | 0.826397 | 2.773851e-54 | -8.615850e-47 |
| Msa0821350 | Msa0966710 | 0.800337 | 1.511101e-48 | -8.615850e-47 |
| Msa0821350 | Msa1000970 | 0.806977 | 6.328425e-50 | -8.615850e-47 |
| Msa0821350 | Msa1008670 | 0.829320 | 5.499239e-55 | -8.615850e-47 |
| Msa0821350 | Msa1033920 | 0.801539 | 8.584934e-49 | -8.615850e-47 |
| Msa0821350 | Msa1071410 | 0.840592 | 7.925257e-58 | -8.615850e-47 |
| Msa0821350 | Msa1082380 | 0.801896 | 7.253255e-49 | -8.615850e-47 |
| Msa0821350 | Msa1106220 | 0.803577 | 3.261456e-49 | -8.615850e-47 |
| Msa0821350 | Msa1126620 | 0.801192 | 1.011345e-48 | -8.615850e-47 |
| Msa0821350 | Msa1162880 | 0.805984 | 1.024976e-49 | -8.615850e-47 |
| Msa0821350 | Msa1206040 | 0.803314 | 3.698973e-49 | -8.615850e-47 |
| Msa0821350 | Msa1215980 | 0.823152 | 1.615794e-53 | -8.615850e-47 |
| Msa0821350 | Msa1240940 | 0.818770 | 1.648357e-52 | -8.615850e-47 |
| Msa0821350 | Msa1254420 | 0.808298 | 3.317325e-50 | -8.615850e-47 |
| Msa0821350 | Msa1266690 | 0.824065 | 9.877698e-54 | -8.615850e-47 |
| Msa0821350 | Msa1267930 | 0.818417 | 1.982180e-52 | -8.615850e-47 |
| Msa0821350 | Msa1282300 | 0.804203 | 2.417469e-49 | -8.615850e-47 |
| Msa0821350 | Msa1294460 | 0.801848 | 7.417254e-49 | -8.615850e-47 |
| Msa0821350 | Msa1294790 | 0.830238 | 3.285622e-55 | -8.615850e-47 |
| Msa0821350 | Msa1297070 | 0.812925 | 3.317292e-51 | -8.615850e-47 |
| Msa0821350 | Msa1301820 | 0.806413 | 8.324863e-50 | -8.615850e-47 |
| Msa0821350 | Msa1328870 | 0.800559 | 1.361968e-48 | -8.615850e-47 |
| Msa0821350 | Msa1333360 | 0.821681 | 3.547572e-53 | -8.615850e-47 |
| Msa0821350 | Msa1361930 | 0.801424 | 9.063563e-49 | -8.615850e-47 |
| Msa0821350 | Msa1372030 | 0.808530 | 2.959821e-50 | -8.615850e-47 |
| Msa0821350 | Msa1381800 | 0.836692 | 8.061877e-57 | -8.615850e-47 |
| Msa0821350 | Msa1387500 | 0.806337 | 8.640665e-50 | -8.615850e-47 |
| Msa0821350 | Msa1389820 | 0.808835 | 2.548008e-50 | -8.615850e-47 |
| Msa0821350 | Msa1393560 | 0.844455 | 7.480526e-59 | -8.615850e-47 |
| Msa0821350 | Msa1426320 | 0.822949 | 1.801441e-53 | -8.615850e-47 |
| Msa0125590 | Msa0821350 | 0.826239 | 3.025147e-54 | -8.615850e-47 |
| Msa0156050 | Msa0821350 | 0.803219 | 3.869423e-49 | -8.615850e-47 |
| Msa0156090 | Msa0821350 | 0.811314 | 7.449754e-51 | -8.615850e-47 |
| Msa0168380 | Msa0821350 | 0.813041 | 3.128383e-51 | -8.615850e-47 |
| Msa0170070 | Msa0821350 | 0.810301 | 1.233778e-50 | -8.615850e-47 |
| Msa0172590 | Msa0821350 | 0.803498 | 3.387141e-49 | -8.615850e-47 |
| Msa0204970 | Msa0821350 | 0.814264 | 1.682977e-51 | -8.615850e-47 |
| Msa0373170 | Msa0821350 | 0.837156 | 6.138278e-57 | -8.615850e-47 |
| Msa0416210 | Msa0821350 | 0.824438 | 8.072210e-54 | -8.615850e-47 |
| Msa0421750 | Msa0821350 | 0.816693 | 4.849612e-52 | -8.615850e-47 |
| Msa0433630 | Msa0821350 | 0.833453 | 5.287371e-56 | -8.615850e-47 |
| Msa0467690 | Msa0821350 | 0.834404 | 3.057371e-56 | -8.615850e-47 |
| Msa0249870 | Msa0821350 | 0.806320 | 8.711803e-50 | -8.615850e-47 |
| Msa0281540 | Msa0821350 | 0.821901 | 3.155502e-53 | -8.615850e-47 |
| Msa0320580 | Msa0821350 | 0.819668 | 1.029302e-52 | -8.615850e-47 |
| Msa0344110 | Msa0821350 | 0.821999 | 2.994678e-53 | -8.615850e-47 |
| Msa0506540 | Msa0821350 | 0.809131 | 2.201746e-50 | -8.615850e-47 |
| Msa0507810 | Msa0821350 | 0.829649 | 4.575096e-55 | -8.615850e-47 |
| Msa0507820 | Msa0821350 | 0.822487 | 2.307246e-53 | -8.615850e-47 |
| Msa0507920 | Msa0821350 | 0.834757 | 2.492457e-56 | -8.615850e-47 |
| Msa0514020 | Msa0821350 | 0.829937 | 3.892442e-55 | -8.615850e-47 |
| Msa0521510 | Msa0821350 | 0.823327 | 1.470275e-53 | -8.615850e-47 |
| Msa0527850 | Msa0821350 | 0.803751 | 3.002646e-49 | -8.615850e-47 |
| Msa0541740 | Msa0821350 | 0.820340 | 7.223206e-53 | -8.615850e-47 |
| Msa0544410 | Msa0821350 | 0.812026 | 5.214967e-51 | -8.615850e-47 |
| Msa0554300 | Msa0821350 | 0.818876 | 1.559298e-52 | -8.615850e-47 |
| Msa0561560 | Msa0821350 | 0.817828 | 2.693512e-52 | -8.615850e-47 |
| Msa0571350 | Msa0821350 | 0.815232 | 1.027302e-51 | -8.615850e-47 |
| Msa0572830 | Msa0821350 | 0.848271 | 6.818066e-60 | -8.615850e-47 |
| Msa0582460 | Msa0821350 | 0.804836 | 1.784406e-49 | -8.615850e-47 |
| Msa0584530 | Msa0821350 | 0.805820 | 1.109768e-49 | -8.615850e-47 |
| Msa0586200 | Msa0821350 | 0.801303 | 9.596651e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821350 | MtrunA17_Chr5g0441531 | 92.520 | 254 | 1 | 1 | 1 | 236 | 1 | 254 | 1.41e-173 | 477 |
| Msa0821350 | MtrunA17_Chr3g0134031 | 44.118 | 340 | 78 | 13 | 1 | 232 | 1 | 336 | 8.22e-75 | 231 |
| Msa0821350 | MtrunA17_Chr4g0004151 | 98.795 | 83 | 1 | 0 | 154 | 236 | 1 | 83 | 5.81e-54 | 168 |
| Msa0821350 | MtrunA17_Chr5g0397881 | 37.445 | 227 | 97 | 4 | 15 | 197 | 14 | 239 | 5.04e-40 | 140 |
| Msa0821350 | MtrunA17_Chr7g0257331 | 38.806 | 201 | 95 | 3 | 18 | 200 | 15 | 205 | 6.01e-38 | 132 |
| Msa0821350 | MtrunA17_Chr1g0203601 | 34.274 | 248 | 120 | 6 | 17 | 228 | 10 | 250 | 4.52e-37 | 132 |
| Msa0821350 | MtrunA17_Chr6g0472421 | 33.585 | 265 | 125 | 7 | 16 | 231 | 24 | 286 | 3.62e-34 | 125 |
| Msa0821350 | MtrunA17_Chr5g0402291 | 33.061 | 245 | 116 | 7 | 18 | 224 | 2 | 236 | 9.02e-32 | 117 |
| Msa0821350 | MtrunA17_Chr8g0375041 | 33.068 | 251 | 114 | 7 | 18 | 236 | 2 | 230 | 2.68e-31 | 115 |
| Msa0821350 | MtrunA17_Chr5g0410881 | 38.743 | 191 | 87 | 3 | 22 | 194 | 29 | 207 | 4.30e-29 | 114 |
| Msa0821350 | MtrunA17_Chr6g0467041 | 55.914 | 93 | 23 | 1 | 24 | 98 | 21 | 113 | 1.46e-27 | 107 |
| Msa0821350 | MtrunA17_Chr7g0260331 | 52.128 | 94 | 27 | 1 | 23 | 98 | 47 | 140 | 5.44e-25 | 100 |
| Msa0821350 | MtrunA17_Chr8g0379701 | 54.545 | 99 | 26 | 2 | 18 | 98 | 6 | 103 | 8.82e-25 | 100 |
| Msa0821350 | MtrunA17_Chr8g0378061 | 54.945 | 91 | 23 | 1 | 26 | 98 | 23 | 113 | 3.22e-24 | 100 |
| Msa0821350 | MtrunA17_Chr1g0185131 | 36.842 | 171 | 59 | 4 | 23 | 174 | 30 | 170 | 5.30e-24 | 98.2 |
| Msa0821350 | MtrunA17_Chr1g0204741 | 40.252 | 159 | 57 | 3 | 23 | 163 | 38 | 176 | 6.84e-24 | 97.4 |
| Msa0821350 | MtrunA17_Chr2g0330821 | 50.000 | 102 | 33 | 1 | 15 | 98 | 69 | 170 | 9.51e-23 | 94.7 |
| Msa0821350 | MtrunA17_Chr4g0071051 | 35.329 | 167 | 81 | 4 | 23 | 162 | 99 | 265 | 8.16e-21 | 90.1 |
| Msa0821350 | MtrunA17_Chr4g0047421 | 32.530 | 166 | 82 | 4 | 22 | 165 | 30 | 187 | 2.79e-20 | 85.5 |
| Msa0821350 | MtrunA17_Chr2g0284481 | 46.000 | 100 | 35 | 2 | 18 | 99 | 9 | 107 | 2.90e-20 | 88.2 |
| Msa0821350 | MtrunA17_Chr3g0135831 | 32.704 | 159 | 70 | 2 | 23 | 163 | 44 | 183 | 1.83e-19 | 85.9 |
| Msa0821350 | MtrunA17_Chr4g0039891 | 34.395 | 157 | 72 | 3 | 25 | 163 | 12 | 155 | 1.86e-19 | 86.7 |
| Msa0821350 | MtrunA17_Chr6g0485571 | 43.878 | 98 | 37 | 1 | 19 | 98 | 5 | 102 | 3.43e-19 | 85.1 |
| Msa0821350 | MtrunA17_Chr8g0392001 | 46.875 | 96 | 32 | 2 | 24 | 100 | 21 | 116 | 2.46e-18 | 82.8 |
| Msa0821350 | MtrunA17_Chr1g0179321 | 58.209 | 67 | 26 | 1 | 19 | 83 | 43 | 109 | 2.22e-17 | 75.1 |
| Msa0821350 | MtrunA17_Chr4g0023771 | 60.377 | 53 | 21 | 0 | 25 | 77 | 15 | 67 | 7.80e-16 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821350 | AT1G46264.1 | 54.222 | 225 | 52 | 7 | 16 | 193 | 24 | 244 | 2.03e-69 | 217 |
| Msa0821350 | AT4G36990.1 | 34.524 | 252 | 127 | 5 | 12 | 229 | 1 | 248 | 3.69e-38 | 134 |
| Msa0821350 | AT5G62020.1 | 33.071 | 254 | 133 | 4 | 18 | 235 | 16 | 268 | 3.65e-35 | 127 |
| Msa0821350 | AT4G11660.1 | 56.311 | 103 | 27 | 1 | 17 | 101 | 51 | 153 | 5.62e-33 | 123 |
| Msa0821350 | AT1G32330.2 | 38.043 | 184 | 84 | 4 | 22 | 186 | 34 | 206 | 6.05e-29 | 113 |
| Msa0821350 | AT1G32330.1 | 38.043 | 184 | 84 | 4 | 22 | 186 | 34 | 206 | 6.64e-29 | 113 |
| Msa0821350 | AT2G41690.1 | 35.359 | 181 | 89 | 4 | 23 | 176 | 38 | 217 | 3.83e-28 | 107 |
| Msa0821350 | AT4G17750.1 | 50.467 | 107 | 35 | 1 | 15 | 103 | 42 | 148 | 1.78e-27 | 109 |
| Msa0821350 | AT3G22830.1 | 51.020 | 98 | 30 | 1 | 20 | 99 | 56 | 153 | 3.97e-24 | 99.8 |
| Msa0821350 | AT5G16820.1 | 52.000 | 100 | 28 | 3 | 21 | 102 | 24 | 121 | 1.92e-23 | 98.2 |
| Msa0821350 | AT5G16820.2 | 52.000 | 100 | 28 | 3 | 21 | 102 | 24 | 121 | 1.92e-23 | 98.2 |
| Msa0821350 | AT3G02990.1 | 51.042 | 96 | 28 | 2 | 21 | 98 | 20 | 114 | 1.92e-23 | 98.2 |
| Msa0821350 | AT5G03720.2 | 50.000 | 98 | 31 | 1 | 25 | 104 | 55 | 152 | 5.22e-23 | 96.7 |
| Msa0821350 | AT5G03720.1 | 50.000 | 98 | 31 | 1 | 25 | 104 | 55 | 152 | 5.22e-23 | 96.7 |
| Msa0821350 | AT5G43840.1 | 47.619 | 105 | 37 | 1 | 23 | 109 | 17 | 121 | 6.58e-23 | 94.4 |
| Msa0821350 | AT2G26150.1 | 46.392 | 97 | 34 | 1 | 20 | 98 | 39 | 135 | 2.10e-21 | 91.3 |
| Msa0821350 | AT2G26150.4 | 46.392 | 97 | 34 | 1 | 20 | 98 | 39 | 135 | 2.10e-21 | 91.3 |
| Msa0821350 | AT4G13980.1 | 51.648 | 91 | 26 | 1 | 26 | 98 | 24 | 114 | 5.39e-21 | 91.3 |
| Msa0821350 | AT3G63350.1 | 29.767 | 215 | 112 | 6 | 19 | 200 | 22 | 230 | 1.07e-20 | 88.6 |
| Msa0821350 | AT3G51910.1 | 46.875 | 96 | 33 | 1 | 20 | 97 | 24 | 119 | 1.62e-19 | 85.1 |
| Msa0821350 | AT5G54070.1 | 43.158 | 95 | 36 | 1 | 21 | 97 | 67 | 161 | 3.09e-19 | 85.1 |
| Msa0821350 | AT3G24520.1 | 44.211 | 95 | 35 | 1 | 22 | 98 | 14 | 108 | 4.95e-19 | 84.7 |
| Msa0821350 | AT1G67970.1 | 38.655 | 119 | 54 | 2 | 24 | 123 | 18 | 136 | 6.62e-18 | 82.0 |
| Msa0821350 | AT4G18880.1 | 54.545 | 99 | 26 | 2 | 18 | 98 | 9 | 106 | 7.86e-18 | 82.0 |
| Msa0821350 | AT5G45710.3 | 35.465 | 172 | 85 | 4 | 25 | 173 | 13 | 181 | 1.41e-16 | 78.2 |
| Msa0821350 | AT5G45710.1 | 35.465 | 172 | 85 | 4 | 25 | 173 | 13 | 181 | 1.41e-16 | 78.2 |
| Msa0821350 | AT4G18870.1 | 34.234 | 111 | 53 | 3 | 26 | 119 | 14 | 121 | 4.00e-11 | 62.0 |
Find 34 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATATCAGTGTTTGATTT+AGG | 0.203557 | 5_3:+59474063 | None:intergenic |
| AGCTCTTCTGTGAAGCTATT+TGG | 0.233498 | 5_3:-59473814 | Msa0821350:CDS |
| CTATTTGGTGTTCCTCTTTG+TGG | 0.339191 | 5_3:-59473799 | Msa0821350:CDS |
| TCCTTATCATTTGGTAATTG+AGG | 0.352875 | 5_3:+59473844 | None:intergenic |
| ATGAAACTACATTTGTTGTT+TGG | 0.362655 | 5_3:-59474863 | Msa0821350:CDS |
| CTATTACAATCCTTATCATT+TGG | 0.365932 | 5_3:+59473835 | None:intergenic |
| AGAAGATCTCTAGCAAATTC+AGG | 0.373696 | 5_3:+59474834 | None:intergenic |
| CTTTGTTCAAAGGGTGAAGC+AGG | 0.399996 | 5_3:+59473919 | None:intergenic |
| ATTGTTGCTTCTTTGTTCAA+AGG | 0.403141 | 5_3:+59473909 | None:intergenic |
| TCTTATTAATCAAGATTACT+AGG | 0.404553 | 5_3:+59473760 | None:intergenic |
| TTGTTGCTTCTTTGTTCAAA+GGG | 0.445911 | 5_3:+59473910 | None:intergenic |
| AAACTACATTTGTTGTTTGG+AGG | 0.447885 | 5_3:-59474860 | Msa0821350:CDS |
| TGGTATGTCTTTGTCAAGAA+AGG | 0.453363 | 5_3:+59474933 | None:intergenic |
| ATTGTTGTTGATAGTGTTGA+GGG | 0.457477 | 5_3:+59474157 | None:intergenic |
| AGATAACATGGTGTTCACTA+TGG | 0.459097 | 5_3:-59474979 | Msa0821350:CDS |
| ACCTCAATTACCAAATGATA+AGG | 0.460161 | 5_3:-59473845 | Msa0821350:CDS |
| TTTGATTTAGGAGATGGTAA+TGG | 0.467405 | 5_3:+59474075 | None:intergenic |
| ATCCAACAGATACTTTCATC+TGG | 0.487646 | 5_3:+59474105 | None:intergenic |
| TCAGTGTTTGATTTAGGAGA+TGG | 0.490616 | 5_3:+59474069 | None:intergenic |
| TCACTGACCACATTGTATCT+TGG | 0.491347 | 5_3:-59474893 | Msa0821350:CDS |
| GTCTTTGTCAAGAAAGGAGC+AGG | 0.498324 | 5_3:+59474939 | None:intergenic |
| AACCAGATGAAAGTATCTGT+TGG | 0.501425 | 5_3:-59474107 | Msa0821350:CDS |
| CTGAGGATAATCAAAGACTA+AGG | 0.512230 | 5_3:-59474029 | Msa0821350:CDS |
| ATCTATTATGTGAAATCCAC+AGG | 0.520532 | 5_3:-59474188 | Msa0821350:CDS |
| TATCTTAACAGCACTTTCTG+AGG | 0.572199 | 5_3:-59474046 | Msa0821350:CDS |
| TATTGTTGTTGATAGTGTTG+AGG | 0.584009 | 5_3:+59474156 | None:intergenic |
| TGAGAGGATCATCAACAAGT+TGG | 0.584975 | 5_3:+59474913 | None:intergenic |
| GATACAATGTGGTCAGTGAG+AGG | 0.630246 | 5_3:+59474897 | None:intergenic |
| TGATGGCTTTCACATTAGAG+AGG | 0.643157 | 5_3:-59475007 | Msa0821350:CDS |
| AGAGAGGTGTGAAGATAACA+TGG | 0.644284 | 5_3:-59474991 | Msa0821350:CDS |
| TTGTTGTTGATAGTGTTGAG+GGG | 0.651120 | 5_3:+59474158 | None:intergenic |
| AGATCTCTAGCAAATTCAGG+AGG | 0.698266 | 5_3:+59474837 | None:intergenic |
| AGGGTGAAGCAGGACTCACA+TGG | 0.700311 | 5_3:+59473929 | None:intergenic |
| CATCACTCCAAGATACAATG+TGG | 0.717706 | 5_3:+59474886 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTAGAAATTATATGAAAA+TGG | + | chr5_3:59474252-59474271 | None:intergenic | 15.0% |
| !! | GTTTAGAAATTATATGAAAA+TGG | + | chr5_3:59474252-59474271 | None:intergenic | 15.0% |
| !! | TAACTATAATAATGAACTGA+AGG | + | chr5_3:59474085-59474104 | None:intergenic | 20.0% |
| !! | TTATGAAAATATGCTGAAAA+CGG | - | chr5_3:59474298-59474317 | Msa0821350:intron | 20.0% |
| !! | TAACTATAATAATGAACTGA+AGG | + | chr5_3:59474085-59474104 | None:intergenic | 20.0% |
| !! | TTATGAAAATATGCTGAAAA+CGG | - | chr5_3:59474298-59474317 | Msa0821350:intron | 20.0% |
| !!! | ATGAAACTACATTTGTTGTT+TGG | - | chr5_3:59473907-59473926 | Msa0821350:CDS | 25.0% |
| ! | TTGTTGTGCTTGAAAAAATT+GGG | + | chr5_3:59473963-59473982 | None:intergenic | 25.0% |
| ! | GTACTAAAACAATATCAAGA+AGG | + | chr5_3:59474059-59474078 | None:intergenic | 25.0% |
| !!! | ATAAGCTATTTCTGTAACAA+AGG | - | chr5_3:59474210-59474229 | Msa0821350:CDS | 25.0% |
| ! | CTGACAATAATTTGAGAAAA+TGG | - | chr5_3:59474385-59474404 | Msa0821350:intron | 25.0% |
| ! | GCAAATGATTACTTCAAAAA+AGG | - | chr5_3:59474551-59474570 | Msa0821350:intron | 25.0% |
| !! | AAGATATCAGTGTTTGATTT+AGG | + | chr5_3:59474710-59474729 | None:intergenic | 25.0% |
| !!! | TATTTTGAAGCTTGTTGAAT+TGG | - | chr5_3:59474892-59474911 | Msa0821350:CDS | 25.0% |
| ! | CTATTACAATCCTTATCATT+TGG | + | chr5_3:59474938-59474957 | None:intergenic | 25.0% |
| !!! | ATGAAACTACATTTGTTGTT+TGG | - | chr5_3:59473907-59473926 | Msa0821350:CDS | 25.0% |
| ! | TTGTTGTGCTTGAAAAAATT+GGG | + | chr5_3:59473963-59473982 | None:intergenic | 25.0% |
| ! | GTACTAAAACAATATCAAGA+AGG | + | chr5_3:59474059-59474078 | None:intergenic | 25.0% |
| !!! | ATAAGCTATTTCTGTAACAA+AGG | - | chr5_3:59474210-59474229 | Msa0821350:CDS | 25.0% |
| ! | CTGACAATAATTTGAGAAAA+TGG | - | chr5_3:59474385-59474404 | Msa0821350:intron | 25.0% |
| ! | GCAAATGATTACTTCAAAAA+AGG | - | chr5_3:59474551-59474570 | Msa0821350:intron | 25.0% |
| !! | AAGATATCAGTGTTTGATTT+AGG | + | chr5_3:59474710-59474729 | None:intergenic | 25.0% |
| !!! | TATTTTGAAGCTTGTTGAAT+TGG | - | chr5_3:59474892-59474911 | Msa0821350:CDS | 25.0% |
| ! | CTATTACAATCCTTATCATT+TGG | + | chr5_3:59474938-59474957 | None:intergenic | 25.0% |
| !! | AAACTACATTTGTTGTTTGG+AGG | - | chr5_3:59473910-59473929 | Msa0821350:CDS | 30.0% |
| GTTGTTGTGCTTGAAAAAAT+TGG | + | chr5_3:59473964-59473983 | None:intergenic | 30.0% | |
| AATTATTGTCAGTGCTTCAT+AGG | + | chr5_3:59474377-59474396 | None:intergenic | 30.0% | |
| AATTTGAGAAAATGGAACAG+CGG | - | chr5_3:59474393-59474412 | Msa0821350:intron | 30.0% | |
| ATCTATTATGTGAAATCCAC+AGG | - | chr5_3:59474582-59474601 | Msa0821350:intron | 30.0% | |
| !! | ATTGTTGTTGATAGTGTTGA+GGG | + | chr5_3:59474616-59474635 | None:intergenic | 30.0% |
| !! | TATTGTTGTTGATAGTGTTG+AGG | + | chr5_3:59474617-59474636 | None:intergenic | 30.0% |
| TCATCTGGTTGAAAAATTTG+TGG | + | chr5_3:59474653-59474672 | None:intergenic | 30.0% | |
| ! | TTTGATTTAGGAGATGGTAA+TGG | + | chr5_3:59474698-59474717 | None:intergenic | 30.0% |
| ! | TTGTTGCTTCTTTGTTCAAA+GGG | + | chr5_3:59474863-59474882 | None:intergenic | 30.0% |
| ! | ATTGTTGCTTCTTTGTTCAA+AGG | + | chr5_3:59474864-59474883 | None:intergenic | 30.0% |
| ACCTCAATTACCAAATGATA+AGG | - | chr5_3:59474925-59474944 | Msa0821350:CDS | 30.0% | |
| ! | TCCTTATCATTTGGTAATTG+AGG | + | chr5_3:59474929-59474948 | None:intergenic | 30.0% |
| !! | AAACTACATTTGTTGTTTGG+AGG | - | chr5_3:59473910-59473929 | Msa0821350:CDS | 30.0% |
| GTTGTTGTGCTTGAAAAAAT+TGG | + | chr5_3:59473964-59473983 | None:intergenic | 30.0% | |
| AATTATTGTCAGTGCTTCAT+AGG | + | chr5_3:59474377-59474396 | None:intergenic | 30.0% | |
| AATTTGAGAAAATGGAACAG+CGG | - | chr5_3:59474393-59474412 | Msa0821350:intron | 30.0% | |
| ATCTATTATGTGAAATCCAC+AGG | - | chr5_3:59474582-59474601 | Msa0821350:intron | 30.0% | |
| !! | ATTGTTGTTGATAGTGTTGA+GGG | + | chr5_3:59474616-59474635 | None:intergenic | 30.0% |
| !! | TATTGTTGTTGATAGTGTTG+AGG | + | chr5_3:59474617-59474636 | None:intergenic | 30.0% |
| TCATCTGGTTGAAAAATTTG+TGG | + | chr5_3:59474653-59474672 | None:intergenic | 30.0% | |
| ! | TTTGATTTAGGAGATGGTAA+TGG | + | chr5_3:59474698-59474717 | None:intergenic | 30.0% |
| ! | TTGTTGCTTCTTTGTTCAAA+GGG | + | chr5_3:59474863-59474882 | None:intergenic | 30.0% |
| ! | ATTGTTGCTTCTTTGTTCAA+AGG | + | chr5_3:59474864-59474883 | None:intergenic | 30.0% |
| ACCTCAATTACCAAATGATA+AGG | - | chr5_3:59474925-59474944 | Msa0821350:CDS | 30.0% | |
| ! | TCCTTATCATTTGGTAATTG+AGG | + | chr5_3:59474929-59474948 | None:intergenic | 30.0% |
| AGATAACATGGTGTTCACTA+TGG | - | chr5_3:59473791-59473810 | Msa0821350:CDS | 35.0% | |
| TGGTATGTCTTTGTCAAGAA+AGG | + | chr5_3:59473840-59473859 | None:intergenic | 35.0% | |
| AGAAGATCTCTAGCAAATTC+AGG | + | chr5_3:59473939-59473958 | None:intergenic | 35.0% | |
| !! | ACAACTTCTCAAGCTTTGTT+AGG | - | chr5_3:59473979-59473998 | Msa0821350:CDS | 35.0% |
| CACATGTATCAATGTCATGT+TGG | - | chr5_3:59474424-59474443 | Msa0821350:intron | 35.0% | |
| !! | TTGTTGTTGATAGTGTTGAG+GGG | + | chr5_3:59474615-59474634 | None:intergenic | 35.0% |
| !! | AACCAGATGAAAGTATCTGT+TGG | - | chr5_3:59474663-59474682 | Msa0821350:intron | 35.0% |
| ATCCAACAGATACTTTCATC+TGG | + | chr5_3:59474668-59474687 | None:intergenic | 35.0% | |
| ! | TCAGTGTTTGATTTAGGAGA+TGG | + | chr5_3:59474704-59474723 | None:intergenic | 35.0% |
| TATCTTAACAGCACTTTCTG+AGG | - | chr5_3:59474724-59474743 | Msa0821350:intron | 35.0% | |
| CTGAGGATAATCAAAGACTA+AGG | - | chr5_3:59474741-59474760 | Msa0821350:intron | 35.0% | |
| TTCCTCTTTGTGGAAAAAAG+AGG | - | chr5_3:59474981-59475000 | Msa0821350:CDS | 35.0% | |
| TCCTCTTTGTGGAAAAAAGA+GGG | - | chr5_3:59474982-59475001 | Msa0821350:CDS | 35.0% | |
| AGATAACATGGTGTTCACTA+TGG | - | chr5_3:59473791-59473810 | Msa0821350:CDS | 35.0% | |
| TGGTATGTCTTTGTCAAGAA+AGG | + | chr5_3:59473840-59473859 | None:intergenic | 35.0% | |
| AGAAGATCTCTAGCAAATTC+AGG | + | chr5_3:59473939-59473958 | None:intergenic | 35.0% | |
| !! | ACAACTTCTCAAGCTTTGTT+AGG | - | chr5_3:59473979-59473998 | Msa0821350:CDS | 35.0% |
| CACATGTATCAATGTCATGT+TGG | - | chr5_3:59474424-59474443 | Msa0821350:intron | 35.0% | |
| !! | TTGTTGTTGATAGTGTTGAG+GGG | + | chr5_3:59474615-59474634 | None:intergenic | 35.0% |
| !! | AACCAGATGAAAGTATCTGT+TGG | - | chr5_3:59474663-59474682 | Msa0821350:intron | 35.0% |
| ATCCAACAGATACTTTCATC+TGG | + | chr5_3:59474668-59474687 | None:intergenic | 35.0% | |
| ! | TCAGTGTTTGATTTAGGAGA+TGG | + | chr5_3:59474704-59474723 | None:intergenic | 35.0% |
| TATCTTAACAGCACTTTCTG+AGG | - | chr5_3:59474724-59474743 | Msa0821350:intron | 35.0% | |
| CTGAGGATAATCAAAGACTA+AGG | - | chr5_3:59474741-59474760 | Msa0821350:intron | 35.0% | |
| TTCCTCTTTGTGGAAAAAAG+AGG | - | chr5_3:59474981-59475000 | Msa0821350:CDS | 35.0% | |
| TCCTCTTTGTGGAAAAAAGA+GGG | - | chr5_3:59474982-59475001 | Msa0821350:CDS | 35.0% | |
| ! | AGAGAGGTGTGAAGATAACA+TGG | - | chr5_3:59473779-59473798 | Msa0821350:CDS | 40.0% |
| TGAGAGGATCATCAACAAGT+TGG | + | chr5_3:59473860-59473879 | None:intergenic | 40.0% | |
| TCACTGACCACATTGTATCT+TGG | - | chr5_3:59473877-59473896 | Msa0821350:CDS | 40.0% | |
| CATCACTCCAAGATACAATG+TGG | + | chr5_3:59473887-59473906 | None:intergenic | 40.0% | |
| AGATCTCTAGCAAATTCAGG+AGG | + | chr5_3:59473936-59473955 | None:intergenic | 40.0% | |
| !! | GCTGAAAACGGCTTTTGAAA+AGG | - | chr5_3:59474310-59474329 | Msa0821350:intron | 40.0% |
| TTACGTACTTCTCTTCTTGC+AGG | - | chr5_3:59474493-59474512 | Msa0821350:intron | 40.0% | |
| TACGTACTTCTCTTCTTGCA+GGG | - | chr5_3:59474494-59474513 | Msa0821350:intron | 40.0% | |
| TCAAGAAGGTTGTTGCTGAT+AGG | - | chr5_3:59474519-59474538 | Msa0821350:intron | 40.0% | |
| AGCTCTTCTGTGAAGCTATT+TGG | - | chr5_3:59474956-59474975 | Msa0821350:CDS | 40.0% | |
| !! | CTATTTGGTGTTCCTCTTTG+TGG | - | chr5_3:59474971-59474990 | Msa0821350:CDS | 40.0% |
| !!! | ACCCTCTTTTTTCCACAAAG+AGG | + | chr5_3:59474986-59475005 | None:intergenic | 40.0% |
| ! | AGAGAGGTGTGAAGATAACA+TGG | - | chr5_3:59473779-59473798 | Msa0821350:CDS | 40.0% |
| TGAGAGGATCATCAACAAGT+TGG | + | chr5_3:59473860-59473879 | None:intergenic | 40.0% | |
| TCACTGACCACATTGTATCT+TGG | - | chr5_3:59473877-59473896 | Msa0821350:CDS | 40.0% | |
| CATCACTCCAAGATACAATG+TGG | + | chr5_3:59473887-59473906 | None:intergenic | 40.0% | |
| AGATCTCTAGCAAATTCAGG+AGG | + | chr5_3:59473936-59473955 | None:intergenic | 40.0% | |
| !! | GCTGAAAACGGCTTTTGAAA+AGG | - | chr5_3:59474310-59474329 | Msa0821350:intron | 40.0% |
| TTACGTACTTCTCTTCTTGC+AGG | - | chr5_3:59474493-59474512 | Msa0821350:intron | 40.0% | |
| TACGTACTTCTCTTCTTGCA+GGG | - | chr5_3:59474494-59474513 | Msa0821350:intron | 40.0% | |
| TCAAGAAGGTTGTTGCTGAT+AGG | - | chr5_3:59474519-59474538 | Msa0821350:intron | 40.0% | |
| AGCTCTTCTGTGAAGCTATT+TGG | - | chr5_3:59474956-59474975 | Msa0821350:CDS | 40.0% | |
| !! | CTATTTGGTGTTCCTCTTTG+TGG | - | chr5_3:59474971-59474990 | Msa0821350:CDS | 40.0% |
| !!! | ACCCTCTTTTTTCCACAAAG+AGG | + | chr5_3:59474986-59475005 | None:intergenic | 40.0% |
| GTCTTTGTCAAGAAAGGAGC+AGG | + | chr5_3:59473834-59473853 | None:intergenic | 45.0% | |
| GATACAATGTGGTCAGTGAG+AGG | + | chr5_3:59473876-59473895 | None:intergenic | 45.0% | |
| CTTCTTGCAGGGTTTCAAGA+AGG | - | chr5_3:59474505-59474524 | Msa0821350:intron | 45.0% | |
| AGAAGGTTGTTGCTGATAGG+TGG | - | chr5_3:59474522-59474541 | Msa0821350:intron | 45.0% | |
| GAAGGTTGTTGCTGATAGGT+GGG | - | chr5_3:59474523-59474542 | Msa0821350:intron | 45.0% | |
| CTTTGTTCAAAGGGTGAAGC+AGG | + | chr5_3:59474854-59474873 | None:intergenic | 45.0% | |
| GTCTTTGTCAAGAAAGGAGC+AGG | + | chr5_3:59473834-59473853 | None:intergenic | 45.0% | |
| GATACAATGTGGTCAGTGAG+AGG | + | chr5_3:59473876-59473895 | None:intergenic | 45.0% | |
| CTTCTTGCAGGGTTTCAAGA+AGG | - | chr5_3:59474505-59474524 | Msa0821350:intron | 45.0% | |
| AGAAGGTTGTTGCTGATAGG+TGG | - | chr5_3:59474522-59474541 | Msa0821350:intron | 45.0% | |
| GAAGGTTGTTGCTGATAGGT+GGG | - | chr5_3:59474523-59474542 | Msa0821350:intron | 45.0% | |
| CTTTGTTCAAAGGGTGAAGC+AGG | + | chr5_3:59474854-59474873 | None:intergenic | 45.0% | |
| ! | GTTGAGGGGTTTTTCTCCTG+TGG | + | chr5_3:59474601-59474620 | None:intergenic | 50.0% |
| ! | GTTGAGGGGTTTTTCTCCTG+TGG | + | chr5_3:59474601-59474620 | None:intergenic | 50.0% |
| AGGGTGAAGCAGGACTCACA+TGG | + | chr5_3:59474844-59474863 | None:intergenic | 55.0% | |
| AGGGTGAAGCAGGACTCACA+TGG | + | chr5_3:59474844-59474863 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 59473765 | 59475027 | 59473765 | ID=Msa0821350;Name=Msa0821350 |
| chr5_3 | mRNA | 59473765 | 59475027 | 59473765 | ID=Msa0821350-mRNA-1;Parent=Msa0821350;Name=Msa0821350-mRNA-1;_AED=0.14;_eAED=0.15;_QI=0|0|0|1|0|0.5|2|0|236 |
| chr5_3 | exon | 59474797 | 59475027 | 59474797 | ID=Msa0821350-mRNA-1:exon:18092;Parent=Msa0821350-mRNA-1 |
| chr5_3 | exon | 59473765 | 59474244 | 59473765 | ID=Msa0821350-mRNA-1:exon:18091;Parent=Msa0821350-mRNA-1 |
| chr5_3 | CDS | 59474797 | 59475027 | 59474797 | ID=Msa0821350-mRNA-1:cds;Parent=Msa0821350-mRNA-1 |
| chr5_3 | CDS | 59473765 | 59474244 | 59473765 | ID=Msa0821350-mRNA-1:cds;Parent=Msa0821350-mRNA-1 |
| Gene Sequence |
| Protein sequence |
