AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0798720


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0798720 MtrunA17_Chr2g0294881 70.748 147 27 3 4 136 385 529 6.66e-65 206
Msa0798720 MtrunA17_Chr1g0192541 64.667 150 36 3 4 138 386 533 1.91e-61 197
Msa0798720 MtrunA17_Chr7g0271871 64.430 149 36 3 4 137 386 532 5.52e-60 193
Msa0798720 MtrunA17_Chr8g0346871 66.000 150 35 3 4 139 291 438 3.67e-58 186
Msa0798720 MtrunA17_Chr4g0004921 65.333 150 32 4 4 139 85 228 1.95e-55 173
Msa0798720 MtrunA17_Chr4g0071961 64.844 128 32 2 4 120 24 149 2.54e-50 160
Msa0798720 MtrunA17_Chr4g0074371 67.500 120 28 1 4 112 308 427 4.01e-50 165
Msa0798720 MtrunA17_Chr4g0075691 67.742 124 27 2 5 117 307 428 5.61e-49 164
Msa0798720 MtrunA17_Chr4g0075701 65.625 128 29 2 4 120 319 442 6.80e-49 163
Msa0798720 MtrunA17_Chr4g0068051 57.931 145 44 4 4 136 389 528 1.03e-47 160
Msa0798720 MtrunA17_Chr4g0075651 63.415 123 32 2 4 115 28 148 6.21e-46 146
Msa0798720 MtrunA17_Chr4g0074081 49.359 156 32 1 4 112 306 461 1.78e-39 137
Msa0798720 MtrunA17_Chr1g0174791 55.833 120 42 2 2 110 180 299 1.39e-36 127
Msa0798720 MtrunA17_Chr7g0271821 44.915 118 53 2 4 110 438 554 2.80e-25 99.8
Msa0798720 MtrunA17_Chr4g0075601 52.525 99 23 2 5 79 24 122 6.51e-25 92.8
Msa0798720 MtrunA17_Chr7g0271831 42.857 112 56 3 4 115 310 413 9.28e-19 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0798720 AT3G06720.1 63.758 149 39 3 4 137 384 532 2.89e-57 186
Msa0798720 AT3G06720.2 63.758 149 39 3 4 137 384 532 2.89e-57 186
Msa0798720 AT4G16143.2 62.162 148 39 3 4 136 389 534 1.26e-54 179
Msa0798720 AT4G16143.1 62.162 148 39 3 4 136 389 534 1.26e-54 179
Msa0798720 AT1G09270.3 60.000 135 42 2 4 126 309 443 1.74e-44 151
Msa0798720 AT1G09270.2 60.000 135 42 2 4 126 391 525 1.99e-44 152
Msa0798720 AT1G09270.1 60.000 135 42 2 4 126 391 525 1.99e-44 152
Msa0798720 AT4G02150.1 53.793 145 54 3 4 136 387 530 9.27e-44 150
Msa0798720 AT1G02690.1 53.333 150 53 3 4 136 388 537 2.82e-43 149
Msa0798720 AT1G02690.2 53.333 150 53 3 4 136 389 538 2.98e-43 149
Msa0798720 AT1G32880.1 51.240 121 51 1 4 116 63 183 1.63e-36 124
Msa0798720 AT5G49310.1 60.177 113 37 1 4 108 382 494 5.62e-36 129
Msa0798720 AT5G49305.5 49.587 121 53 1 3 115 263 383 4.04e-33 120
Msa0798720 AT5G49305.3 49.587 121 53 1 3 115 263 383 4.04e-33 120
Msa0798720 AT5G49305.4 49.587 121 53 1 3 115 263 383 4.04e-33 120
Msa0798720 AT5G49305.1 49.587 121 53 1 3 115 263 383 4.04e-33 120
Msa0798720 AT5G49305.2 49.587 121 53 1 3 115 263 383 4.04e-33 120
Msa0798720 AT3G05720.1 49.580 119 42 1 4 104 372 490 4.32e-32 119
Msa0798720 AT3G05720.2 49.580 119 42 1 4 104 372 490 4.32e-32 119
Msa0798720 AT5G52000.1 51.376 109 44 2 5 104 313 421 2.76e-26 102

Find 28 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATATTCTCAAGACATAT+TGG 0.331681 5_3:-11584627 Msa0798720:CDS
TACAAGGTACTTGGCGAGCC+AGG 0.363447 5_3:-11584874 Msa0798720:intron
TCACGTACTTGATTTGTGCC+TGG 0.371058 5_3:+11584984 None:intergenic
ACAGCAACAATTGTTGAATC+AGG 0.374720 5_3:+11584816 None:intergenic
GATAGATAATAACAAGGGTT+TGG 0.407491 5_3:-11584704 Msa0798720:CDS
ATTGTTGCTGTCTGTTTAGA+AGG 0.420778 5_3:-11584804 Msa0798720:CDS
CTATGGAGGCAGTGACCTTC+CGG 0.424887 5_3:-11584542 Msa0798720:CDS
CACGTACTTGATTTGTGCCT+GGG 0.433056 5_3:+11584985 None:intergenic
CCAGGCAATGGTTCTCAACC+TGG 0.441909 5_3:-11584571 Msa0798720:CDS
TGTTGTTATTACAAGGTACT+TGG 0.449681 5_3:-11584883 Msa0798720:intron
CCAGGTTGAGAACCATTGCC+TGG 0.484326 5_3:+11584571 None:intergenic
CTGCTGAAATTGAATCTGTC+CGG 0.490243 5_3:+11584523 None:intergenic
TATTCTCAAGACATATTGGT+TGG 0.495228 5_3:-11584623 Msa0798720:CDS
GCTCTCTCTAATGCTGCATC+TGG 0.498170 5_3:-11585013 Msa0798720:CDS
TGCTGCATCTGGCGGTACCC+AGG 0.528564 5_3:-11585002 Msa0798720:CDS
ACAAGGTACTTGGCGAGCCA+GGG 0.543294 5_3:-11584873 Msa0798720:intron
CTCTCTAATGCTGCATCTGG+CGG 0.574327 5_3:-11585010 Msa0798720:CDS
CAGCAACAATTGTTGAATCA+GGG 0.581439 5_3:+11584817 None:intergenic
CAACAGTGAAATTTATGAGA+AGG 0.597194 5_3:-11584653 Msa0798720:CDS
CTGCCTCCATAGTTAAAACC+AGG 0.599308 5_3:+11584553 None:intergenic
ACAGAGGCTTAATGCAACCC+TGG 0.616878 5_3:+11584856 None:intergenic
GAAGTCGAGACACTACCTCC+AGG 0.636732 5_3:-11584589 Msa0798720:CDS
TGAAATTGAATCTGTCCGGA+AGG 0.640829 5_3:+11584527 None:intergenic
GAGACACTACCTCCAGGCAA+TGG 0.644889 5_3:-11584583 Msa0798720:CDS
TCAGACGATAGATAATAACA+AGG 0.697485 5_3:-11584710 Msa0798720:intron
CAGACGATAGATAATAACAA+GGG 0.718539 5_3:-11584709 Msa0798720:CDS
GGTTGAGAACCATTGCCTGG+AGG 0.737525 5_3:+11584574 None:intergenic
CAAACAAGTAAATCACACAG+AGG 0.794778 5_3:+11584840 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATATTCTCAAGACATAT+TGG - chr5_3:11584957-11584976 Msa0798720:intron 20.0%
! CTTGTAATAACAACAACATT+TGG + chr5_3:11584696-11584715 None:intergenic 25.0%
!! CAAATGTTGTTGTTATTACA+AGG - chr5_3:11584694-11584713 Msa0798720:CDS 25.0%
! TGTTGTTATTACAAGGTACT+TGG - chr5_3:11584701-11584720 Msa0798720:CDS 30.0%
TGGAGAAGTGGATAAAAATT+CGG - chr5_3:11584824-11584843 Msa0798720:CDS 30.0%
TCAGACGATAGATAATAACA+AGG - chr5_3:11584874-11584893 Msa0798720:intron 30.0%
CAGACGATAGATAATAACAA+GGG - chr5_3:11584875-11584894 Msa0798720:intron 30.0%
GATAGATAATAACAAGGGTT+TGG - chr5_3:11584880-11584899 Msa0798720:intron 30.0%
CAACAGTGAAATTTATGAGA+AGG - chr5_3:11584931-11584950 Msa0798720:intron 30.0%
TATTCTCAAGACATATTGGT+TGG - chr5_3:11584961-11584980 Msa0798720:intron 30.0%
ATTTGAAGAAGATTGACCAG+AGG + chr5_3:11584520-11584539 None:intergenic 35.0%
GTTGACATTCAAAAAGAAGC+TGG - chr5_3:11584544-11584563 Msa0798720:CDS 35.0%
ACATTCAAAAAGAAGCTGGA+TGG - chr5_3:11584548-11584567 Msa0798720:CDS 35.0%
CATTCAAAAAGAAGCTGGAT+GGG - chr5_3:11584549-11584568 Msa0798720:CDS 35.0%
CAAACAAGTAAATCACACAG+AGG + chr5_3:11584747-11584766 None:intergenic 35.0%
CAGCAACAATTGTTGAATCA+GGG + chr5_3:11584770-11584789 None:intergenic 35.0%
ACAGCAACAATTGTTGAATC+AGG + chr5_3:11584771-11584790 None:intergenic 35.0%
ATTGTTGCTGTCTGTTTAGA+AGG - chr5_3:11584780-11584799 Msa0798720:CDS 35.0%
! AGGTCTTGAGAATTTTCTGA+AGG - chr5_3:11584800-11584819 Msa0798720:CDS 35.0%
!! CTTGAGAATTTTCTGAAGGT+TGG - chr5_3:11584804-11584823 Msa0798720:CDS 35.0%
GGATAAAAATTCGGTAACAC+CGG - chr5_3:11584833-11584852 Msa0798720:CDS 35.0%
!!! TCTCAACCTGGTTTTAACTA+TGG - chr5_3:11585025-11585044 Msa0798720:CDS 35.0%
!! TTTTCTGAAGGTTGGAGAAG+TGG - chr5_3:11584812-11584831 Msa0798720:CDS 40.0%
! AGCATACAAGTTGACATCTC+CGG + chr5_3:11584855-11584874 None:intergenic 40.0%
!!! CAACCTGGTTTTAACTATGG+AGG - chr5_3:11585028-11585047 Msa0798720:CDS 40.0%
TGAAATTGAATCTGTCCGGA+AGG + chr5_3:11585060-11585079 None:intergenic 40.0%
CTGCTGAAATTGAATCTGTC+CGG + chr5_3:11585064-11585083 None:intergenic 40.0%
!! CACGTACTTGATTTGTGCCT+GGG + chr5_3:11584602-11584621 None:intergenic 45.0%
!! TCACGTACTTGATTTGTGCC+TGG + chr5_3:11584603-11584622 None:intergenic 45.0%
CTGCCTCCATAGTTAAAACC+AGG + chr5_3:11585034-11585053 None:intergenic 45.0%
GCTCTCTCTAATGCTGCATC+TGG - chr5_3:11584571-11584590 Msa0798720:CDS 50.0%
CTCTCTAATGCTGCATCTGG+CGG - chr5_3:11584574-11584593 Msa0798720:CDS 50.0%
TTTCTTGCCCTGCTTCATGC+TGG - chr5_3:11584642-11584661 Msa0798720:CDS 50.0%
AATGTCGCCAGCATGAAGCA+GGG + chr5_3:11584652-11584671 None:intergenic 50.0%
ACAGAGGCTTAATGCAACCC+TGG + chr5_3:11584731-11584750 None:intergenic 50.0%
CAATGTCGCCAGCATGAAGC+AGG + chr5_3:11584653-11584672 None:intergenic 55.0%
! TACAAGGTACTTGGCGAGCC+AGG - chr5_3:11584710-11584729 Msa0798720:intron 55.0%
! ACAAGGTACTTGGCGAGCCA+GGG - chr5_3:11584711-11584730 Msa0798720:intron 55.0%
GAAGTCGAGACACTACCTCC+AGG - chr5_3:11584995-11585014 Msa0798720:CDS 55.0%
GAGACACTACCTCCAGGCAA+TGG - chr5_3:11585001-11585020 Msa0798720:CDS 55.0%
GGTTGAGAACCATTGCCTGG+AGG + chr5_3:11585013-11585032 None:intergenic 55.0%
! CCAGGTTGAGAACCATTGCC+TGG + chr5_3:11585016-11585035 None:intergenic 55.0%
CCAGGCAATGGTTCTCAACC+TGG - chr5_3:11585013-11585032 Msa0798720:CDS 55.0%
CTATGGAGGCAGTGACCTTC+CGG - chr5_3:11585042-11585061 Msa0798720:CDS 55.0%
TGCTGCATCTGGCGGTACCC+AGG - chr5_3:11584582-11584601 Msa0798720:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 11584516 11585090 11584516 ID=Msa0798720;Name=Msa0798720
chr5_3 mRNA 11584516 11585090 11584516 ID=Msa0798720-mRNA-1;Parent=Msa0798720;Name=Msa0798720-mRNA-1;_AED=0.34;_eAED=0.34;_QI=22|0.5|0.33|1|0.5|0.66|3|0|139
chr5_3 exon 11584516 11584728 11584516 ID=Msa0798720-mRNA-1:exon:4776;Parent=Msa0798720-mRNA-1
chr5_3 exon 11584761 11584890 11584761 ID=Msa0798720-mRNA-1:exon:4775;Parent=Msa0798720-mRNA-1
chr5_3 exon 11584992 11585090 11584992 ID=Msa0798720-mRNA-1:exon:4774;Parent=Msa0798720-mRNA-1
chr5_3 five_prime_UTR 11585069 11585090 11585069 ID=Msa0798720-mRNA-1:five_prime_utr;Parent=Msa0798720-mRNA-1
chr5_3 CDS 11584992 11585068 11584992 ID=Msa0798720-mRNA-1:cds;Parent=Msa0798720-mRNA-1
chr5_3 CDS 11584761 11584890 11584761 ID=Msa0798720-mRNA-1:cds;Parent=Msa0798720-mRNA-1
chr5_3 CDS 11584516 11584728 11584516 ID=Msa0798720-mRNA-1:cds;Parent=Msa0798720-mRNA-1
Gene Sequence

>Msa0798720

ATGCGAGTTGACATTCAAAAAGAAGCTGGATGGGCTCTCTCTAATGCTGCATCTGGCGGTACCCAGGCACAAATCAAGTACTTGGCGAGCCAGGGTTGCATTAAGCCTCTGTGTGATTTACTTGTTTGCCCTGATTCAACAATTGTTGCTGTCTGTTTAGAAGGTCTTGAGAATTTTCTGAAGGTTGGAGAAGTGGATAAAAATTCGACGATAGATAATAACAAGGGTTTGGAAAAGATTGAAAACTTGCAGAGTCACGACAACAGTGAAATTTATGAGAAGGCTGTTAATATTCTCAAGACATATTGGTTGGAAGATGAAGATGAAGTCGAGACACTACCTCCAGGCAATGGTTCTCAACCTGGTTTTAACTATGGAGGCAGTGACCTTCCGGACAGATTCAATTTCAGCAGAATGTGA

Protein sequence

>Msa0798720

MRVDIQKEAGWALSNAASGGTQAQIKYLASQGCIKPLCDLLVCPDSTIVAVCLEGLENFLKVGEVDKNSTIDNNKGLEKIENLQSHDNSEIYEKAVNILKTYWLEDEDEVETLPPGNGSQPGFNYGGSDLPDRFNFSRM*