AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577050 MtrunA17_Chr8g0384741 91.697 542 15 1 1 512 1 542 0.0 1009
Msa0577050 MtrunA17_Chr3g0124811 62.857 490 152 2 52 511 1 490 0.0 654
Msa0577050 MtrunA17_Chr1g0159811 63.571 420 123 2 56 445 3 422 0.0 559
Msa0577050 MtrunA17_Chr8g0372381 45.708 466 223 2 73 508 13 478 3.13e-147 431
Msa0577050 MtrunA17_Chr2g0328781 45.094 479 230 3 64 509 15 493 2.09e-144 424
Msa0577050 MtrunA17_Chr4g0032441 48.253 458 204 4 73 497 9 466 2.07e-130 388
Msa0577050 MtrunA17_Chr3g0129271 44.022 184 100 1 211 391 36 219 2.12e-48 168
Msa0577050 MtrunA17_Chr2g0310091 29.310 348 237 7 135 476 193 537 9.89e-40 151
Msa0577050 MtrunA17_Chr2g0294411 26.652 454 295 12 81 508 135 576 3.16e-38 147
Msa0577050 MtrunA17_Chr4g0059521 30.641 359 238 8 133 483 145 500 2.70e-35 138
Msa0577050 MtrunA17_Chr3g0118121 30.423 355 234 7 122 468 136 485 4.56e-34 134
Msa0577050 MtrunA17_Chr4g0059221 28.571 343 235 7 134 469 196 535 9.70e-33 131
Msa0577050 MtrunA17_Chr3g0118081 29.825 342 229 6 135 468 177 515 1.85e-32 130
Msa0577050 MtrunA17_Chr3g0117861 29.598 348 225 8 134 469 194 533 9.55e-31 125
Msa0577050 MtrunA17_Chr8g0390651 27.536 345 240 8 138 475 159 500 1.55e-29 121
Msa0577050 MtrunA17_Chr8g0365221 27.586 348 240 9 130 469 189 532 1.95e-29 121
Msa0577050 MtrunA17_Chr6g0485301 28.000 350 242 7 133 475 153 499 4.42e-28 117
Msa0577050 MtrunA17_Chr8g0390631 26.571 350 247 7 133 475 145 491 1.62e-26 112
Msa0577050 MtrunA17_Chr6g0485311 27.666 347 237 8 138 475 156 497 1.89e-26 112
Msa0577050 MtrunA17_Chr2g0279751 26.750 400 268 12 103 488 121 509 6.59e-24 105
Msa0577050 MtrunA17_Chr4g0009161 46.154 104 52 2 211 310 121 224 3.58e-23 97.8
Msa0577050 MtrunA17_Chr4g0049641 27.762 353 237 9 136 475 174 521 1.49e-22 100
Msa0577050 MtrunA17_Chr4g0074171 27.187 320 216 8 139 451 158 467 9.70e-22 98.2
Msa0577050 MtrunA17_Chr1g0149961 26.562 320 218 8 139 451 158 467 1.95e-20 94.4
Msa0577050 MtrunA17_Chr2g0300801 21.930 342 255 7 136 467 152 491 3.51e-14 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577050 AT4G34240.1 58.000 550 182 4 1 512 10 548 0.0 637
Msa0577050 AT4G34240.4 59.772 527 171 3 16 512 18 533 0.0 634
Msa0577050 AT1G44170.1 59.708 479 163 2 61 509 4 482 0.0 608
Msa0577050 AT1G44170.2 59.708 479 163 2 61 509 4 482 0.0 608
Msa0577050 AT1G44170.3 63.702 416 151 0 94 509 4 419 0.0 580
Msa0577050 AT4G34240.3 67.822 404 130 0 109 512 3 406 0.0 580
Msa0577050 AT4G36250.1 45.666 473 224 4 73 512 12 484 3.55e-147 431
Msa0577050 AT4G34240.2 56.122 392 123 4 1 354 10 390 2.76e-140 410
Msa0577050 AT3G66658.2 27.594 453 292 11 81 508 135 576 5.35e-41 155
Msa0577050 AT1G79440.1 32.551 341 219 9 135 468 184 520 7.99e-41 154
Msa0577050 AT3G66658.1 27.941 408 269 8 81 473 135 532 1.40e-40 154
Msa0577050 AT1G74920.2 30.939 362 236 10 122 474 131 487 8.35e-37 142
Msa0577050 AT1G74920.1 30.939 362 236 10 122 474 136 492 8.50e-37 142
Msa0577050 AT3G48170.1 28.291 357 245 8 135 483 147 500 4.59e-31 126
Msa0577050 AT1G23800.1 27.666 347 241 8 130 469 182 525 1.84e-29 122
Msa0577050 AT3G48000.1 25.959 443 284 12 65 469 93 529 1.42e-28 119
Msa0577050 AT3G24503.1 26.686 341 240 7 136 469 155 492 1.23e-26 113
Msa0577050 AT2G14170.2 27.174 368 248 11 136 489 137 498 2.76e-19 91.3
Msa0577050 AT2G14170.1 27.371 369 246 12 136 489 246 607 3.03e-19 91.7
Msa0577050 AT2G24270.2 25.392 319 223 7 139 451 198 507 3.94e-19 90.9
Msa0577050 AT2G24270.4 25.392 319 223 7 139 451 158 467 7.05e-19 90.1
Msa0577050 AT2G24270.3 25.392 319 223 7 139 451 158 467 7.70e-19 89.7
Msa0577050 AT2G24270.1 25.392 319 223 7 139 451 158 467 7.70e-19 89.7
Msa0577050 AT1G54100.2 23.894 339 246 9 139 467 154 490 8.13e-18 86.7
Msa0577050 AT1G54100.1 23.894 339 246 9 139 467 154 490 8.13e-18 86.7
Msa0577050 AT2G14170.3 27.451 306 205 9 136 429 246 546 1.01e-17 86.7
Msa0577050 AT1G23800.2 35.185 162 95 4 130 281 182 343 1.42e-15 79.3

Find 129 sgRNAs with CRISPR-Local

Find 287 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACACAGATAGCAGCTTTAA+AGG 0.191916 4_1:+81220303 Msa0577050:intron
GTTATGCAAGAGGAGATATT+TGG 0.197595 4_1:+81220617 Msa0577050:CDS
GATTGCTCCAACTATCTTAT+TGG 0.199481 4_1:+81220574 Msa0577050:CDS
TCCAGGAGTGCAGTTGTTTC+AGG 0.225324 4_1:-81219378 None:intergenic
AGTATATGGTTGGTGAAGTT+TGG 0.247638 4_1:-81217390 None:intergenic
ACAGTAATAATTGGCAAAAT+TGG 0.249721 4_1:-81220641 None:intergenic
GCAACTCGTGGTTTGCCTTT+TGG 0.265536 4_1:+81221486 Msa0577050:CDS
TATATAAAACTGATTTCTTA+TGG 0.269097 4_1:-81221569 None:intergenic
AGCAGCTTTAAAGGAAGAAT+TGG 0.292732 4_1:+81220312 Msa0577050:CDS
ATAAATTTATTCACAGTGAT+TGG 0.311303 4_1:-81221844 None:intergenic
ATTTAAGGACGAAAAGTATA+TGG 0.319662 4_1:-81217404 None:intergenic
ATGGTACTCACAGTAATAAT+TGG 0.320472 4_1:-81220650 None:intergenic
CACTGCGTTGCCCGCGGAAA+TGG 0.344228 4_1:-81219250 None:intergenic
CAGATAATTCTTGCTTTGCT+TGG 0.345687 4_1:+81221684 Msa0577050:CDS
ATTCACAGTGATTGGGCCTC+TGG 0.347371 4_1:-81221836 None:intergenic
CCATGGACTCACTTTAAGTT+AGG 0.361327 4_1:+81217476 Msa0577050:five_prime_UTR
GAAAATAAACAAGTATTTGA+TGG 0.363766 4_1:+81218031 Msa0577050:CDS
TCCTGAAACAACTGCACTCC+TGG 0.366164 4_1:+81219377 Msa0577050:CDS
GAAATAGAATTCAAGGTTGT+TGG 0.372438 4_1:-81217555 None:intergenic
AAAGTATCTTATCCCACTTC+TGG 0.373046 4_1:-81219401 None:intergenic
TAATTCTTGCTTTGCTTGGA+TGG 0.383020 4_1:+81221688 Msa0577050:CDS
GTATATGGTTGGTGAAGTTT+GGG 0.386054 4_1:-81217389 None:intergenic
TGTATATGGAGGGTACCTTA+TGG 0.386947 4_1:-81221613 None:intergenic
TCAACTAGAAATAGAATTCA+AGG 0.390007 4_1:-81217562 None:intergenic
CGAATGGCGGATTTCCCAAT+TGG 0.395995 4_1:+81218120 Msa0577050:CDS
TCTTCACTATGGCCTCCAAT+TGG 0.399929 4_1:-81218135 None:intergenic
GCAAGCAGGGATGATGTTGC+AGG 0.410513 4_1:-81219297 None:intergenic
TATGTGGACAAGATATCTTC+TGG 0.414474 4_1:+81220847 Msa0577050:CDS
TAAATTTATTCACAGTGATT+GGG 0.421081 4_1:-81221843 None:intergenic
GCGGAAATGGCTCCAATGAC+TGG 0.421715 4_1:-81219237 None:intergenic
TTATCATTGGATCCAGTCAT+TGG 0.424102 4_1:+81219225 Msa0577050:CDS
AGATATGCAGCCAAAGGCTT+TGG 0.424118 4_1:-81220793 None:intergenic
GGAGGATAATAGCTGGCAAA+TGG 0.425073 4_1:+81220140 Msa0577050:CDS
CCTACCTGCAGTGTTATCAT+TGG 0.425905 4_1:+81219212 Msa0577050:intron
AAAATCAAGGCCAAAGCCTT+TGG 0.435468 4_1:+81220783 Msa0577050:CDS
GGCAAATGGGCATGCAATAG+TGG 0.448828 4_1:+81220154 Msa0577050:CDS
AGAGTTGAAATGAAGTTGCT+TGG 0.457326 4_1:-81217845 None:intergenic
TTATGCAAGAGGAGATATTT+GGG 0.457790 4_1:+81220618 Msa0577050:CDS
TAGAGTAGGTCGCATTGTTA+TGG 0.459468 4_1:+81219934 Msa0577050:CDS
GAAACTCTTGAATGAGGATA+AGG 0.463840 4_1:+81220414 Msa0577050:CDS
ACTCCAGTGATTCTTGAGCT+TGG 0.463911 4_1:+81219977 Msa0577050:CDS
GACACAATCTCTGCTGATGA+TGG 0.464544 4_1:-81218831 None:intergenic
CAAGGTTTGAGGAAGAACTT+TGG 0.464714 4_1:-81217610 None:intergenic
TTTCTAGCAAATTTGCAAGC+AGG 0.466192 4_1:-81219311 None:intergenic
CTTCACTATGGCCTCCAATT+GGG 0.466557 4_1:-81218134 None:intergenic
GGTTGGTGAAGTTTGGGTAA+TGG 0.468925 4_1:-81217383 None:intergenic
CTTTGCTTGTTAGAGAGCTC+AGG 0.469563 4_1:+81218065 Msa0577050:CDS
GTCATTGGAGCCATTTCCGC+GGG 0.470626 4_1:+81219240 Msa0577050:CDS
TTTGGAGGAGTTGGAGAAAG+TGG 0.471512 4_1:+81221504 Msa0577050:CDS
TTCTAGCAAATTTGCAAGCA+GGG 0.472320 4_1:-81219310 None:intergenic
ACATTTGAATCAACAACAGC+TGG 0.482725 4_1:-81220010 None:intergenic
TTCCGCCATTGCTTGCTCCT+AGG 0.482912 4_1:+81217656 Msa0577050:CDS
TATCAGTCTCTGATCTCTGA+TGG 0.485074 4_1:+81221744 Msa0577050:three_prime_UTR
TTGATTCCGATCCATCCATA+AGG 0.489103 4_1:+81221598 Msa0577050:CDS
TCTTATCCCACTTCTGGTCC+AGG 0.490487 4_1:-81219395 None:intergenic
ACGAGTCGTGCATATTGAGT+TGG 0.490817 4_1:-81220391 None:intergenic
GGAGTTGGAGAAAGTGGAAT+GGG 0.490988 4_1:+81221510 Msa0577050:CDS
CACTTCAAGAACTGAAACAC+TGG 0.491328 4_1:+81218692 Msa0577050:CDS
CAAGGTTGTTGGTGATAATG+AGG 0.492261 4_1:-81217544 None:intergenic
TCCTTCTCAGGTGTATATGG+AGG 0.493263 4_1:-81221624 None:intergenic
CAAAGGCTCTGCTACCAACC+AGG 0.493432 4_1:-81217795 None:intergenic
CCTTCTCAGGTGTATATGGA+GGG 0.495634 4_1:-81221623 None:intergenic
GAGTTGAAATGAAGTTGCTT+GGG 0.495918 4_1:-81217844 None:intergenic
GGAACATCCAATAAGATAGT+TGG 0.497071 4_1:-81220581 None:intergenic
CAACACAACTCCTAGTGGTT+CGG 0.497401 4_1:-81218853 None:intergenic
CCTCCAAGCTCAAGAATCAC+TGG 0.501775 4_1:-81219980 None:intergenic
CTGCACTCCTGGACCAGAAG+TGG 0.504845 4_1:+81219388 Msa0577050:CDS
ATCAGTCTCTGATCTCTGAT+GGG 0.511456 4_1:+81221745 Msa0577050:three_prime_UTR
AGAGCCAATCTTACAAGCGT+TGG 0.514311 4_1:-81220226 None:intergenic
AAACAATGAGCAACTGAAGA+AGG 0.517946 4_1:+81220822 Msa0577050:CDS
TGTTGTTAAATTGTTTGCAA+AGG 0.518370 4_1:-81217812 None:intergenic
AGTTCTTCATAACAGTGCCC+TGG 0.518511 4_1:+81217777 Msa0577050:intron
AGTGGAATGGGTTGCTACCA+TGG 0.521895 4_1:+81221522 Msa0577050:CDS
GGATGTTCCACAAGAAGCTA+TGG 0.525082 4_1:+81220595 Msa0577050:CDS
TGCATAACCATAGCTTCTTG+TGG 0.525176 4_1:-81220602 None:intergenic
TCATAGTAAAACAAGGTTTG+AGG 0.525805 4_1:-81217621 None:intergenic
GAGGATAATAGCTGGCAAAT+GGG 0.531521 4_1:+81220141 Msa0577050:CDS
ATGGCGGATTTCCCAATTGG+AGG 0.535856 4_1:+81218123 Msa0577050:CDS
TCTGCAGTAAGAGTTGTTGA+AGG 0.537984 4_1:+81219348 Msa0577050:CDS
CATCACATGAAGATTTCGCC+TGG 0.539665 4_1:-81218666 None:intergenic
CCAGTGATTCTTGAGCTTGG+AGG 0.540889 4_1:+81219980 Msa0577050:CDS
CTTTCTCCAACTCCTCCAAA+AGG 0.541724 4_1:-81221501 None:intergenic
AAGGACGAAAAGTATATGGT+TGG 0.549183 4_1:-81217400 None:intergenic
ACACTGGATGAAACCTGAAA+AGG 0.549602 4_1:+81218708 Msa0577050:CDS
AGGAGTTGGAGAAAGTGGAA+TGG 0.554064 4_1:+81221509 Msa0577050:CDS
AGATTATGCAACATAGTCCA+TGG 0.555313 4_1:+81217459 Msa0577050:five_prime_UTR
GTGAAGTTTGGGTAATGGAA+AGG 0.557326 4_1:-81217378 None:intergenic
CCTAACTTAAAGTGAGTCCA+TGG 0.563870 4_1:-81217476 None:intergenic
TATGGAGGGTACCTTATGGA+TGG 0.573766 4_1:-81221609 None:intergenic
TATCAAAAGAGAACTTTCCA+TGG 0.574054 4_1:-81221539 None:intergenic
GCTTTAGTATAATAAAATCA+AGG 0.574080 4_1:+81220770 Msa0577050:CDS
GGTTTGAGGAAGAACTTTGG+TGG 0.575323 4_1:-81217607 None:intergenic
CATTTCCGCGGGCAACGCAG+TGG 0.577408 4_1:+81219251 Msa0577050:CDS
CTGCAGTAAGAGTTGTTGAA+GGG 0.582509 4_1:+81219349 Msa0577050:CDS
AGAAGCTATGGTTATGCAAG+AGG 0.586940 4_1:+81220607 Msa0577050:CDS
GCAACTGAAGAAGGATTATG+TGG 0.588911 4_1:+81220831 Msa0577050:CDS
TTTGTAAGGTTGAGGACCAG+AGG 0.589148 4_1:+81221820 Msa0577050:three_prime_UTR
CCCTCCATATACACCTGAGA+AGG 0.591816 4_1:+81221623 Msa0577050:CDS
AGGGTACCTTATGGATGGAT+CGG 0.593890 4_1:-81221604 None:intergenic
GAGATTGTGTCCGAACCACT+AGG 0.597033 4_1:+81218843 Msa0577050:CDS
AGTCATTGGAGCCATTTCCG+CGG 0.600289 4_1:+81219239 Msa0577050:CDS
TTGGTGCAGGTTGCAACTCG+TGG 0.603431 4_1:+81221474 Msa0577050:intron
CTTCATAACAGTGCCCTGGT+TGG 0.604404 4_1:+81217781 Msa0577050:intron
ACTGCGAGGAGGATAATAGC+TGG 0.604969 4_1:+81220133 Msa0577050:CDS
CAAAACCACTGCGTTGCCCG+CGG 0.605069 4_1:-81219256 None:intergenic
GTGAAGAGATATGCAGCCAA+AGG 0.606873 4_1:-81220799 None:intergenic
GTGTTATGCAACTCTATCTG+TGG 0.608199 4_1:+81217997 Msa0577050:CDS
ACTCGTGAAACTCTTGAATG+AGG 0.610650 4_1:+81220408 Msa0577050:CDS
TGGACAAGATATCTTCTGGA+GGG 0.613322 4_1:+81220851 Msa0577050:CDS
TGCACTCCTGGACCAGAAGT+GGG 0.619653 4_1:+81219389 Msa0577050:CDS
TGAAATTTCAGTTCACTGCG+AGG 0.626166 4_1:+81220119 Msa0577050:intron
TACCTAGGAGCAAGCAATGG+CGG 0.626311 4_1:-81217658 None:intergenic
CAAGCAATGGCGGAAGACAG+AGG 0.629441 4_1:-81217648 None:intergenic
TACTCAGGTAGTGCTAGAGT+AGG 0.631910 4_1:+81219920 Msa0577050:intron
TGCAGTAAGAGTTGTTGAAG+GGG 0.632245 4_1:+81219350 Msa0577050:CDS
AGACAAAGAGCTACGAATGG+CGG 0.643657 4_1:+81218107 Msa0577050:CDS
AAAGGCTCTGCTACCAACCA+GGG 0.646284 4_1:-81217794 None:intergenic
GTGGACAAGATATCTTCTGG+AGG 0.652280 4_1:+81220850 Msa0577050:CDS
GGTCTAAATGATGATGTGCA+AGG 0.655883 4_1:+81221709 Msa0577050:exon
GTCTAAATGATGATGTGCAA+GGG 0.657116 4_1:+81221710 Msa0577050:exon
ATGATCAACACAACTCCTAG+TGG 0.658303 4_1:-81218858 None:intergenic
TCAGTCTCTGATCTCTGATG+GGG 0.664207 4_1:+81221746 Msa0577050:three_prime_UTR
GCAAGACAAAGAGCTACGAA+TGG 0.673266 4_1:+81218104 Msa0577050:CDS
ATGTACCTAGGAGCAAGCAA+TGG 0.680273 4_1:-81217661 None:intergenic
AATTTCAGTTCACTGCGAGG+AGG 0.681260 4_1:+81220122 Msa0577050:intron
TTGTGTTGATCATATCAACA+TGG 0.681886 4_1:+81218868 Msa0577050:CDS
GTTTGGGTAATGGAAAGGAG+TGG 0.684376 4_1:-81217373 None:intergenic
GTTGCAGGAGAAACTTCTGA+TGG 0.692797 4_1:-81219282 None:intergenic
TGGGATAAGATACTTTACAC+AGG 0.716272 4_1:+81219408 Msa0577050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAACAAAACAAAAAATT+TGG + chr4_1:81217507-81217526 Msa0577050:five_prime_UTR 10.0%
!! TTATTAATGTTAATAATTGT+TGG + chr4_1:81219728-81219747 Msa0577050:intron 10.0%
!! ATAAAAAAGTTACATATATT+TGG + chr4_1:81219844-81219863 Msa0577050:intron 10.0%
!!! AAACATTTTTTTAATATACG+AGG - chr4_1:81217439-81217458 None:intergenic 15.0%
!! AATTGTAATTTCATCATAAT+TGG + chr4_1:81217739-81217758 Msa0577050:intron 15.0%
!!! CTTAATTTATCCTTTTATAT+AGG - chr4_1:81218422-81218441 None:intergenic 15.0%
!! CACAAAATAACAATTATAAA+TGG - chr4_1:81218516-81218535 None:intergenic 15.0%
!!! CATTTATAATTGTTATTTTG+TGG + chr4_1:81218514-81218533 Msa0577050:intron 15.0%
!!! AGTTTTTATCTTTTCTTTTT+GGG + chr4_1:81219115-81219134 Msa0577050:intron 15.0%
!!! TATATAAAACTGATTTCTTA+TGG - chr4_1:81221572-81221591 None:intergenic 15.0%
!!! ATTTTTTTAATATACGAGGA+GGG - chr4_1:81217435-81217454 None:intergenic 20.0%
!!! TTTTTGTTTTGTTTTTTGAG+AGG - chr4_1:81217504-81217523 None:intergenic 20.0%
!! GTAAAGAAACAAAAAGTAAA+TGG - chr4_1:81217693-81217712 None:intergenic 20.0%
!!! TGATGAAATTACAATTTTCT+TGG - chr4_1:81217735-81217754 None:intergenic 20.0%
!! TTAATTCTATTTGCTTAAGA+AGG + chr4_1:81217936-81217955 Msa0577050:intron 20.0%
!!! ACAATAATGTTCTTTTTATG+TGG + chr4_1:81217960-81217979 Msa0577050:intron 20.0%
!! GAAAATAAACAAGTATTTGA+TGG + chr4_1:81218031-81218050 Msa0577050:CDS 20.0%
!! ATAAACAATAGAAACAAAAG+AGG - chr4_1:81218259-81218278 None:intergenic 20.0%
!! TAAGATATTTACAATGCTAA+TGG - chr4_1:81218586-81218605 None:intergenic 20.0%
!! TAAATTGCATTTGGTTATAA+CGG + chr4_1:81218936-81218955 Msa0577050:intron 20.0%
!! TTTGTGAAATTATATTTGAG+TGG - chr4_1:81219045-81219064 None:intergenic 20.0%
!!! CAGTTTTTATCTTTTCTTTT+TGG + chr4_1:81219114-81219133 Msa0577050:intron 20.0%
!! TAAAATCTTTATCAATGGAA+AGG - chr4_1:81219189-81219208 None:intergenic 20.0%
!! AATATAAACAACAAGTTCAA+GGG - chr4_1:81220056-81220075 None:intergenic 20.0%
!! AAATATAAACAACAAGTTCA+AGG - chr4_1:81220057-81220076 None:intergenic 20.0%
!! TATAGATGAAAATATGTTAC+TGG - chr4_1:81220091-81220110 None:intergenic 20.0%
!! GCTTTAGTATAATAAAATCA+AGG + chr4_1:81220770-81220789 Msa0577050:CDS 20.0%
!! TTTCATAAATCTATTGTTCT+CGG + chr4_1:81220969-81220988 Msa0577050:intron 20.0%
!! TTCTCAAATTATTATTGTTG+TGG - chr4_1:81221274-81221293 None:intergenic 20.0%
!! AACCTTACAAAAAAAAATGT+AGG - chr4_1:81221811-81221830 None:intergenic 20.0%
!! TAAATTTATTCACAGTGATT+GGG - chr4_1:81221846-81221865 None:intergenic 20.0%
!! ATAAATTTATTCACAGTGAT+TGG - chr4_1:81221847-81221866 None:intergenic 20.0%
!! ATTTAAGGACGAAAAGTATA+TGG - chr4_1:81217407-81217426 None:intergenic 25.0%
!!! CATTTTTTTAATATACGAGG+AGG - chr4_1:81217436-81217455 None:intergenic 25.0%
!! AACAAAAAATTTGGTAGTCA+AGG + chr4_1:81217516-81217535 Msa0577050:five_prime_UTR 25.0%
! TCAACTAGAAATAGAATTCA+AGG - chr4_1:81217565-81217584 None:intergenic 25.0%
! TGTTGTTAAATTGTTTGCAA+AGG - chr4_1:81217815-81217834 None:intergenic 25.0%
! TTATGTGGTTTATATTGTGA+AGG + chr4_1:81217975-81217994 Msa0577050:intron 25.0%
!! ATTTATCCTTTTATATAGGC+TGG - chr4_1:81218418-81218437 None:intergenic 25.0%
! CAAACTTATTGCCAATAAAT+GGG + chr4_1:81218489-81218508 Msa0577050:intron 25.0%
! TTATAAATGGACCCATTTAT+TGG - chr4_1:81218503-81218522 None:intergenic 25.0%
!! AGGTAATTAAATTGATGGTA+AGG - chr4_1:81218610-81218629 None:intergenic 25.0%
! AAAATGTACACACATAGAAA+CGG - chr4_1:81218633-81218652 None:intergenic 25.0%
!! TTCTTTGTAATTACCTTTTC+AGG - chr4_1:81218724-81218743 None:intergenic 25.0%
!! TTTCTTTTTCTCCTTCTTTT+AGG + chr4_1:81218785-81218804 Msa0577050:intron 25.0%
! ATATATATACTACATGACAG+TGG - chr4_1:81218988-81219007 None:intergenic 25.0%
! TATATTAATGTCCGAAAACT+TGG + chr4_1:81219005-81219024 Msa0577050:intron 25.0%
! GGAGATAAAATCTTTATCAA+TGG - chr4_1:81219194-81219213 None:intergenic 25.0%
! AGATATGCAATGATTTCATA+GGG - chr4_1:81219468-81219487 None:intergenic 25.0%
! TAGATATGCAATGATTTCAT+AGG - chr4_1:81219469-81219488 None:intergenic 25.0%
! ATAAAACCTTATAGCAGAAT+TGG - chr4_1:81219883-81219902 None:intergenic 25.0%
!! ATAATAGACCAATTTTACTC+AGG + chr4_1:81219905-81219924 Msa0577050:intron 25.0%
!!! AAGAATTGGAGCAATTTTTT+GGG + chr4_1:81220326-81220345 Msa0577050:CDS 25.0%
!! GTATCAGACAAAATTGTTTT+AGG + chr4_1:81220436-81220455 Msa0577050:CDS 25.0%
! ACAGTAATAATTGGCAAAAT+TGG - chr4_1:81220644-81220663 None:intergenic 25.0%
!!! AACATTTACTTTTCCCTTTA+TGG - chr4_1:81220672-81220691 None:intergenic 25.0%
! TAATCCCTTAATTTCATCAA+AGG + chr4_1:81221107-81221126 Msa0577050:intron 25.0%
! TCTACCTTTGATGAAATTAA+GGG - chr4_1:81221114-81221133 None:intergenic 25.0%
! TTCTACCTTTGATGAAATTA+AGG - chr4_1:81221115-81221134 None:intergenic 25.0%
!! TTACGATCTAAATTTTGTAC+AGG + chr4_1:81221175-81221194 Msa0577050:intron 25.0%
! AAATATCACATTGACACATT+TGG + chr4_1:81221309-81221328 Msa0577050:intron 25.0%
! ATGTAGTACATATCTATGTA+TGG + chr4_1:81221388-81221407 Msa0577050:intron 25.0%
! TCATGTCTATATCTTACATT+TGG - chr4_1:81221419-81221438 None:intergenic 25.0%
!! TGAGAAGGAAAAAATTTTGA+GGG + chr4_1:81221638-81221657 Msa0577050:CDS 25.0%
! AAAAATATTGCCACCAAAAA+TGG - chr4_1:81221664-81221683 None:intergenic 25.0%
!!! CTCCTACATTTTTTTTTGTA+AGG + chr4_1:81221806-81221825 Msa0577050:three_prime_UTR 25.0%
!!! CATTTTTTTTTGTAAGGTTG+AGG + chr4_1:81221812-81221831 Msa0577050:three_prime_UTR 25.0%
GAAATAGAATTCAAGGTTGT+TGG - chr4_1:81217558-81217577 None:intergenic 30.0%
TCATAGTAAAACAAGGTTTG+AGG - chr4_1:81217624-81217643 None:intergenic 30.0%
! AGGCTTTTCATAGTAAAACA+AGG - chr4_1:81217631-81217650 None:intergenic 30.0%
AAATGGAAAAAGATGTACCT+AGG - chr4_1:81217676-81217695 None:intergenic 30.0%
! TTTTCTTGGCAACATATTTG+TGG - chr4_1:81217721-81217740 None:intergenic 30.0%
GTTATATATGCTTCTAGTCT+AGG - chr4_1:81218217-81218236 None:intergenic 30.0%
TGGTAAAATAAGAGCAATTG+TGG - chr4_1:81218361-81218380 None:intergenic 30.0%
CGATGTGAATCTTTATATTG+TGG - chr4_1:81218381-81218400 None:intergenic 30.0%
AGGATAAATTAAGCTCTTCA+AGG + chr4_1:81218429-81218448 Msa0577050:intron 30.0%
CCATTTATTGGCAATAAGTT+TGG - chr4_1:81218491-81218510 None:intergenic 30.0%
CCAAACTTATTGCCAATAAA+TGG + chr4_1:81218488-81218507 Msa0577050:intron 30.0%
TAAATGCCATCATAACAAGA+GGG - chr4_1:81218553-81218572 None:intergenic 30.0%
AACGGAGGTAATTAAATTGA+TGG - chr4_1:81218615-81218634 None:intergenic 30.0%
! ATTTTAAATGCAGATTGTCC+AGG + chr4_1:81218648-81218667 Msa0577050:intron 30.0%
! TTGTGTTGATCATATCAACA+TGG + chr4_1:81218868-81218887 Msa0577050:CDS 30.0%
AACATGTTCTACATACACAT+AGG - chr4_1:81218900-81218919 None:intergenic 30.0%
CAGACATTCTAAATTGCATT+TGG + chr4_1:81218927-81218946 Msa0577050:intron 30.0%
! GAACTATAGTTCCAAGTTTT+CGG - chr4_1:81219019-81219038 None:intergenic 30.0%
TCTTTATCAATGGAAAGGAA+AGG - chr4_1:81219184-81219203 None:intergenic 30.0%
!! GGGTATTGATGTGATAATAT+AGG - chr4_1:81219448-81219467 None:intergenic 30.0%
ATAATGTGATTGAGTTAGAG+TGG + chr4_1:81219514-81219533 Msa0577050:intron 30.0%
TGGTTAATAAGCTCCAAATA+AGG + chr4_1:81219534-81219553 Msa0577050:intron 30.0%
!! TATTTGGTCTTTTAACACTC+CGG - chr4_1:81219671-81219690 None:intergenic 30.0%
CTTAAGAGTTAAGACTTTAG+AGG - chr4_1:81219763-81219782 None:intergenic 30.0%
!! CTCTAAAGTCTTAACTCTTA+AGG + chr4_1:81219761-81219780 Msa0577050:intron 30.0%
!!! GAAGAATTGGAGCAATTTTT+TGG + chr4_1:81220325-81220344 Msa0577050:CDS 30.0%
!!! ATTTTTTGGGAAAGATCTGA+TGG + chr4_1:81220339-81220358 Msa0577050:CDS 30.0%
!!! TCAGACAAAATTGTTTTAGG+AGG + chr4_1:81220439-81220458 Msa0577050:CDS 30.0%
TCCATCTTGGTTATAAAGAT+TGG + chr4_1:81220496-81220515 Msa0577050:intron 30.0%
TCCAATCTTTATAACCAAGA+TGG - chr4_1:81220500-81220519 None:intergenic 30.0%
TTATGCAAGAGGAGATATTT+GGG + chr4_1:81220618-81220637 Msa0577050:CDS 30.0%
ATGGTACTCACAGTAATAAT+TGG - chr4_1:81220653-81220672 None:intergenic 30.0%
TATTACTGTGAGTACCATAA+AGG + chr4_1:81220655-81220674 Msa0577050:intron 30.0%
ATTACTGTGAGTACCATAAA+GGG + chr4_1:81220656-81220675 Msa0577050:intron 30.0%
TACTACATTTGATGAATGAG+AGG - chr4_1:81221376-81221395 None:intergenic 30.0%
GTAAGATATAGACATGATGA+TGG + chr4_1:81221422-81221441 Msa0577050:intron 30.0%
ACTTGTTTGTACAACAATGT+TGG + chr4_1:81221455-81221474 Msa0577050:intron 30.0%
TATCAAAAGAGAACTTTCCA+TGG - chr4_1:81221542-81221561 None:intergenic 30.0%
!!! CTCAAAATTTTTTCCTTCTC+AGG - chr4_1:81221639-81221658 None:intergenic 30.0%
! CTGAGAAGGAAAAAATTTTG+AGG + chr4_1:81221637-81221656 Msa0577050:CDS 30.0%
!! AAAATTTTGAGGGCCATTTT+TGG + chr4_1:81221648-81221667 Msa0577050:CDS 30.0%
ACAAAAAAAAATGTAGGAGC+AGG - chr4_1:81221805-81221824 None:intergenic 30.0%
!! GTATATGGTTGGTGAAGTTT+GGG - chr4_1:81217392-81217411 None:intergenic 35.0%
!! AGTATATGGTTGGTGAAGTT+TGG - chr4_1:81217393-81217412 None:intergenic 35.0%
! AAGGACGAAAAGTATATGGT+TGG - chr4_1:81217403-81217422 None:intergenic 35.0%
AGATTATGCAACATAGTCCA+TGG + chr4_1:81217459-81217478 Msa0577050:five_prime_UTR 35.0%
GAGTTGAAATGAAGTTGCTT+GGG - chr4_1:81217847-81217866 None:intergenic 35.0%
AGAGTTGAAATGAAGTTGCT+TGG - chr4_1:81217848-81217867 None:intergenic 35.0%
CTCTTCTAACATCTTCACTA+TGG - chr4_1:81218149-81218168 None:intergenic 35.0%
GATGTTAGAAGAGAAAGAGA+AGG + chr4_1:81218156-81218175 Msa0577050:intron 35.0%
CTTCTAGTCTAGGTTTAGAA+AGG - chr4_1:81218207-81218226 None:intergenic 35.0%
! ACTAGAAGCATATATAACCG+AGG + chr4_1:81218219-81218238 Msa0577050:intron 35.0%
AGGAAATTGATTGAAGACCT+CGG - chr4_1:81218239-81218258 None:intergenic 35.0%
TCAGAACCAGCCTATATAAA+AGG + chr4_1:81218409-81218428 Msa0577050:intron 35.0%
! AGTTTGGTTTCTCTTAAGCA+AGG - chr4_1:81218475-81218494 None:intergenic 35.0%
CTAAATGCCATCATAACAAG+AGG - chr4_1:81218554-81218573 None:intergenic 35.0%
! TGAAGTACTCACCTAAAAGA+AGG - chr4_1:81218799-81218818 None:intergenic 35.0%
TTATCATTGGATCCAGTCAT+TGG + chr4_1:81219225-81219244 Msa0577050:CDS 35.0%
TTCTAGCAAATTTGCAAGCA+GGG - chr4_1:81219313-81219332 None:intergenic 35.0%
! TTTCTAGCAAATTTGCAAGC+AGG - chr4_1:81219314-81219333 None:intergenic 35.0%
! AAAGTATCTTATCCCACTTC+TGG - chr4_1:81219404-81219423 None:intergenic 35.0%
TGGGATAAGATACTTTACAC+AGG + chr4_1:81219408-81219427 Msa0577050:CDS 35.0%
AAAAGATTCATCAAACCGTG+CGG + chr4_1:81219488-81219507 Msa0577050:intron 35.0%
CAATCACATTATTATCCGCA+CGG - chr4_1:81219506-81219525 None:intergenic 35.0%
TTGAACGATTCGTCCTTATT+TGG - chr4_1:81219550-81219569 None:intergenic 35.0%
ATCGTAGCTGGAATAATCAT+TGG + chr4_1:81219586-81219605 Msa0577050:intron 35.0%
TGGTAATCGGGAATTTAAGT+AGG - chr4_1:81219629-81219648 None:intergenic 35.0%
TACTTAAATTCCCGATTACC+AGG + chr4_1:81219628-81219647 Msa0577050:intron 35.0%
TAGTCGCAAAGACTGATATT+TGG - chr4_1:81219687-81219706 None:intergenic 35.0%
! AGAGTTAAGACTTTAGAGGA+GGG - chr4_1:81219759-81219778 None:intergenic 35.0%
! AAGAGTTAAGACTTTAGAGG+AGG - chr4_1:81219760-81219779 None:intergenic 35.0%
TAGCACTACCTGAGTAAAAT+TGG - chr4_1:81219916-81219935 None:intergenic 35.0%
ACATTTGAATCAACAACAGC+TGG - chr4_1:81220013-81220032 None:intergenic 35.0%
! TACACAGATAGCAGCTTTAA+AGG + chr4_1:81220303-81220322 Msa0577050:intron 35.0%
!! AGCAGCTTTAAAGGAAGAAT+TGG + chr4_1:81220312-81220331 Msa0577050:CDS 35.0%
GAAACTCTTGAATGAGGATA+AGG + chr4_1:81220414-81220433 Msa0577050:CDS 35.0%
!!! AAATTGTTTTAGGAGGTCAG+AGG + chr4_1:81220446-81220465 Msa0577050:CDS 35.0%
!!! AATTGTTTTAGGAGGTCAGA+GGG + chr4_1:81220447-81220466 Msa0577050:CDS 35.0%
AAGAGGGAGAATTGATGTTA+TGG - chr4_1:81220543-81220562 None:intergenic 35.0%
CAATCCTTCTGCAATAAAGA+GGG - chr4_1:81220559-81220578 None:intergenic 35.0%
GATTGCTCCAACTATCTTAT+TGG + chr4_1:81220574-81220593 Msa0577050:CDS 35.0%
GGAACATCCAATAAGATAGT+TGG - chr4_1:81220584-81220603 None:intergenic 35.0%
GTTATGCAAGAGGAGATATT+TGG + chr4_1:81220617-81220636 Msa0577050:CDS 35.0%
! TCTTTTCCTCACATTTTTGC+AGG + chr4_1:81220729-81220748 Msa0577050:intron 35.0%
AAACAATGAGCAACTGAAGA+AGG + chr4_1:81220822-81220841 Msa0577050:CDS 35.0%
TATGTGGACAAGATATCTTC+TGG + chr4_1:81220847-81220866 Msa0577050:CDS 35.0%
ATCAGTAGACAGAAAAGGAA+AGG + chr4_1:81221000-81221019 Msa0577050:intron 35.0%
TCAAAGGTAGAAACATATGC+AGG + chr4_1:81221123-81221142 Msa0577050:intron 35.0%
! TTTTCCTTCTCAGGTGTATA+TGG - chr4_1:81221630-81221649 None:intergenic 35.0%
CAGATAATTCTTGCTTTGCT+TGG + chr4_1:81221684-81221703 Msa0577050:CDS 35.0%
TAATTCTTGCTTTGCTTGGA+TGG + chr4_1:81221688-81221707 Msa0577050:CDS 35.0%
! GTCTAAATGATGATGTGCAA+GGG + chr4_1:81221710-81221729 Msa0577050:exon 35.0%
!! GTGAAGTTTGGGTAATGGAA+AGG - chr4_1:81217381-81217400 None:intergenic 40.0%
!!! ACGAGGAGGGTTTTGATTTA+AGG - chr4_1:81217422-81217441 None:intergenic 40.0%
CCTAACTTAAAGTGAGTCCA+TGG - chr4_1:81217479-81217498 None:intergenic 40.0%
! CCATGGACTCACTTTAAGTT+AGG + chr4_1:81217476-81217495 Msa0577050:five_prime_UTR 40.0%
!! CAAGGTTGTTGGTGATAATG+AGG - chr4_1:81217547-81217566 None:intergenic 40.0%
CAAGGTTTGAGGAAGAACTT+TGG - chr4_1:81217613-81217632 None:intergenic 40.0%
GTGTTATGCAACTCTATCTG+TGG + chr4_1:81217997-81218016 Msa0577050:CDS 40.0%
!! CTCAGGAAGAATTTTGACAC+TGG + chr4_1:81218082-81218101 Msa0577050:CDS 40.0%
GTATTTCAGTTGTCGCATTG+CGG - chr4_1:81218306-81218325 None:intergenic 40.0%
GTTTCTCTTAAGCAAGGTCT+TGG - chr4_1:81218469-81218488 None:intergenic 40.0%
! TCCATTCCCTCTTGTTATGA+TGG + chr4_1:81218544-81218563 Msa0577050:intron 40.0%
ATGTACACACATAGAAACGG+AGG - chr4_1:81218630-81218649 None:intergenic 40.0%
CACTTCAAGAACTGAAACAC+TGG + chr4_1:81218692-81218711 Msa0577050:CDS 40.0%
ACACTGGATGAAACCTGAAA+AGG + chr4_1:81218708-81218727 Msa0577050:CDS 40.0%
ATGATCAACACAACTCCTAG+TGG - chr4_1:81218861-81218880 None:intergenic 40.0%
CATGACAGTGGTGAATGATA+TGG - chr4_1:81218976-81218995 None:intergenic 40.0%
TCTGCAGTAAGAGTTGTTGA+AGG + chr4_1:81219348-81219367 Msa0577050:CDS 40.0%
CTGCAGTAAGAGTTGTTGAA+GGG + chr4_1:81219349-81219368 Msa0577050:CDS 40.0%
TGCAGTAAGAGTTGTTGAAG+GGG + chr4_1:81219350-81219369 Msa0577050:CDS 40.0%
TTCCAGCTACGATTCAAACT+CGG - chr4_1:81219579-81219598 None:intergenic 40.0%
! GAGTTAAGACTTTAGAGGAG+GGG - chr4_1:81219758-81219777 None:intergenic 40.0%
AGCAGAATTGGCATGCTAAT+TGG - chr4_1:81219871-81219890 None:intergenic 40.0%
AGCATGCCAATTCTGCTATA+AGG + chr4_1:81219874-81219893 Msa0577050:intron 40.0%
TAGAGTAGGTCGCATTGTTA+TGG + chr4_1:81219934-81219953 Msa0577050:CDS 40.0%
TGAAATTTCAGTTCACTGCG+AGG + chr4_1:81220119-81220138 Msa0577050:intron 40.0%
GAGGATAATAGCTGGCAAAT+GGG + chr4_1:81220141-81220160 Msa0577050:CDS 40.0%
ACTCGTGAAACTCTTGAATG+AGG + chr4_1:81220408-81220427 Msa0577050:CDS 40.0%
GTGAGTTTGCATTTCCATCT+TGG + chr4_1:81220483-81220502 Msa0577050:intron 40.0%
TTCTCCCTCTTTATTGCAGA+AGG + chr4_1:81220552-81220571 Msa0577050:intron 40.0%
GCAATCCTTCTGCAATAAAG+AGG - chr4_1:81220560-81220579 None:intergenic 40.0%
TGCATAACCATAGCTTCTTG+TGG - chr4_1:81220605-81220624 None:intergenic 40.0%
AGAAGCTATGGTTATGCAAG+AGG + chr4_1:81220607-81220626 Msa0577050:CDS 40.0%
TGTCTACCTGCAAAAATGTG+AGG - chr4_1:81220738-81220757 None:intergenic 40.0%
AAAATCAAGGCCAAAGCCTT+TGG + chr4_1:81220783-81220802 Msa0577050:CDS 40.0%
GCAACTGAAGAAGGATTATG+TGG + chr4_1:81220831-81220850 Msa0577050:CDS 40.0%
TGGACAAGATATCTTCTGGA+GGG + chr4_1:81220851-81220870 Msa0577050:CDS 40.0%
GTTGCATCAGTAGACAGAAA+AGG + chr4_1:81220995-81221014 Msa0577050:intron 40.0%
TTGTACAACAATGTTGGTGC+AGG + chr4_1:81221461-81221480 Msa0577050:intron 40.0%
TTGATTCCGATCCATCCATA+AGG + chr4_1:81221598-81221617 Msa0577050:CDS 40.0%
!! TGTATATGGAGGGTACCTTA+TGG - chr4_1:81221616-81221635 None:intergenic 40.0%
!!! ATTTTGAGGGCCATTTTTGG+TGG + chr4_1:81221651-81221670 Msa0577050:CDS 40.0%
! GGTCTAAATGATGATGTGCA+AGG + chr4_1:81221709-81221728 Msa0577050:exon 40.0%
TATCAGTCTCTGATCTCTGA+TGG + chr4_1:81221744-81221763 Msa0577050:three_prime_UTR 40.0%
ATCAGTCTCTGATCTCTGAT+GGG + chr4_1:81221745-81221764 Msa0577050:three_prime_UTR 40.0%
! GATGGGGTGTGTTTTTCTAT+AGG + chr4_1:81221762-81221781 Msa0577050:three_prime_UTR 40.0%
!! GTTTGGGTAATGGAAAGGAG+TGG - chr4_1:81217376-81217395 None:intergenic 45.0%
!! GGTTGGTGAAGTTTGGGTAA+TGG - chr4_1:81217386-81217405 None:intergenic 45.0%
GGTTTGAGGAAGAACTTTGG+TGG - chr4_1:81217610-81217629 None:intergenic 45.0%
ATGTACCTAGGAGCAAGCAA+TGG - chr4_1:81217664-81217683 None:intergenic 45.0%
! GGGCACTGTTATGAAGAACT+AGG - chr4_1:81217777-81217796 None:intergenic 45.0%
AGTTCTTCATAACAGTGCCC+TGG + chr4_1:81217777-81217796 Msa0577050:intron 45.0%
! CTTTGCTTGTTAGAGAGCTC+AGG + chr4_1:81218065-81218084 Msa0577050:CDS 45.0%
GCAAGACAAAGAGCTACGAA+TGG + chr4_1:81218104-81218123 Msa0577050:CDS 45.0%
AGACAAAGAGCTACGAATGG+CGG + chr4_1:81218107-81218126 Msa0577050:CDS 45.0%
CTTCACTATGGCCTCCAATT+GGG - chr4_1:81218137-81218156 None:intergenic 45.0%
TCTTCACTATGGCCTCCAAT+TGG - chr4_1:81218138-81218157 None:intergenic 45.0%
GCCATCATAACAAGAGGGAA+TGG - chr4_1:81218548-81218567 None:intergenic 45.0%
CATCACATGAAGATTTCGCC+TGG - chr4_1:81218669-81218688 None:intergenic 45.0%
GACACAATCTCTGCTGATGA+TGG - chr4_1:81218834-81218853 None:intergenic 45.0%
CAACACAACTCCTAGTGGTT+CGG - chr4_1:81218856-81218875 None:intergenic 45.0%
CCAATGATAACACTGCAGGT+AGG - chr4_1:81219215-81219234 None:intergenic 45.0%
CCTACCTGCAGTGTTATCAT+TGG + chr4_1:81219212-81219231 Msa0577050:intron 45.0%
GGATCCAATGATAACACTGC+AGG - chr4_1:81219219-81219238 None:intergenic 45.0%
GTTGCAGGAGAAACTTCTGA+TGG - chr4_1:81219285-81219304 None:intergenic 45.0%
ATCCGAGTTTGAATCGTAGC+TGG + chr4_1:81219574-81219593 Msa0577050:intron 45.0%
TACTCAGGTAGTGCTAGAGT+AGG + chr4_1:81219920-81219939 Msa0577050:intron 45.0%
ACTCCAGTGATTCTTGAGCT+TGG + chr4_1:81219977-81219996 Msa0577050:CDS 45.0%
AATTTCAGTTCACTGCGAGG+AGG + chr4_1:81220122-81220141 Msa0577050:intron 45.0%
GGAGGATAATAGCTGGCAAA+TGG + chr4_1:81220140-81220159 Msa0577050:CDS 45.0%
AGAGCCAATCTTACAAGCGT+TGG - chr4_1:81220229-81220248 None:intergenic 45.0%
ACGAGTCGTGCATATTGAGT+TGG - chr4_1:81220394-81220413 None:intergenic 45.0%
GGATGTTCCACAAGAAGCTA+TGG + chr4_1:81220595-81220614 Msa0577050:CDS 45.0%
AGATATGCAGCCAAAGGCTT+TGG - chr4_1:81220796-81220815 None:intergenic 45.0%
GTGAAGAGATATGCAGCCAA+AGG - chr4_1:81220802-81220821 None:intergenic 45.0%
GTGGACAAGATATCTTCTGG+AGG + chr4_1:81220850-81220869 Msa0577050:CDS 45.0%
GTCGAGTGACTGTGTTTCAT+AGG - chr4_1:81221230-81221249 None:intergenic 45.0%
TGAAACACAGTCACTCGACA+CGG + chr4_1:81221231-81221250 Msa0577050:intron 45.0%
CTTTCTCCAACTCCTCCAAA+AGG - chr4_1:81221504-81221523 None:intergenic 45.0%
TTTGGAGGAGTTGGAGAAAG+TGG + chr4_1:81221504-81221523 Msa0577050:CDS 45.0%
AGGAGTTGGAGAAAGTGGAA+TGG + chr4_1:81221509-81221528 Msa0577050:CDS 45.0%
GGAGTTGGAGAAAGTGGAAT+GGG + chr4_1:81221510-81221529 Msa0577050:CDS 45.0%
! AGGGTACCTTATGGATGGAT+CGG - chr4_1:81221607-81221626 None:intergenic 45.0%
!! TATGGAGGGTACCTTATGGA+TGG - chr4_1:81221612-81221631 None:intergenic 45.0%
CCTTCTCAGGTGTATATGGA+GGG - chr4_1:81221626-81221645 None:intergenic 45.0%
TCCTTCTCAGGTGTATATGG+AGG - chr4_1:81221627-81221646 None:intergenic 45.0%
TCAGTCTCTGATCTCTGATG+GGG + chr4_1:81221746-81221765 Msa0577050:three_prime_UTR 45.0%
TTTGTAAGGTTGAGGACCAG+AGG + chr4_1:81221820-81221839 Msa0577050:three_prime_UTR 45.0%
TACCTAGGAGCAAGCAATGG+CGG - chr4_1:81217661-81217680 None:intergenic 50.0%
CTTCATAACAGTGCCCTGGT+TGG + chr4_1:81217781-81217800 Msa0577050:intron 50.0%
AAAGGCTCTGCTACCAACCA+GGG - chr4_1:81217797-81217816 None:intergenic 50.0%
!! CGAATGGCGGATTTCCCAAT+TGG + chr4_1:81218120-81218139 Msa0577050:CDS 50.0%
!! ATGGCGGATTTCCCAATTGG+AGG + chr4_1:81218123-81218142 Msa0577050:CDS 50.0%
GAGATTGTGTCCGAACCACT+AGG + chr4_1:81218843-81218862 Msa0577050:CDS 50.0%
AGTCATTGGAGCCATTTCCG+CGG + chr4_1:81219239-81219258 Msa0577050:CDS 50.0%
TCCTGAAACAACTGCACTCC+TGG + chr4_1:81219377-81219396 Msa0577050:CDS 50.0%
! TCCAGGAGTGCAGTTGTTTC+AGG - chr4_1:81219381-81219400 None:intergenic 50.0%
TCTTATCCCACTTCTGGTCC+AGG - chr4_1:81219398-81219417 None:intergenic 50.0%
AGAGAACGGGTCCTGGTAAT+CGG - chr4_1:81219642-81219661 None:intergenic 50.0%
ATTACCAGGACCCGTTCTCT+TGG + chr4_1:81219642-81219661 Msa0577050:intron 50.0%
TTACCAGGACCCGTTCTCTT+GGG + chr4_1:81219643-81219662 Msa0577050:intron 50.0%
CCTCCAAGCTCAAGAATCAC+TGG - chr4_1:81219983-81220002 None:intergenic 50.0%
CCAGTGATTCTTGAGCTTGG+AGG + chr4_1:81219980-81219999 Msa0577050:CDS 50.0%
ACTGCGAGGAGGATAATAGC+TGG + chr4_1:81220133-81220152 Msa0577050:CDS 50.0%
GGCAAATGGGCATGCAATAG+TGG + chr4_1:81220154-81220173 Msa0577050:CDS 50.0%
CGCTCCAACGCTTGTAAGAT+TGG + chr4_1:81220222-81220241 Msa0577050:intron 50.0%
!! GCAACTCGTGGTTTGCCTTT+TGG + chr4_1:81221486-81221505 Msa0577050:CDS 50.0%
!! ACTCGTGGTTTGCCTTTTGG+AGG + chr4_1:81221489-81221508 Msa0577050:CDS 50.0%
!! GGTTTGCCTTTTGGAGGAGT+TGG + chr4_1:81221495-81221514 Msa0577050:CDS 50.0%
!! AGTGGAATGGGTTGCTACCA+TGG + chr4_1:81221522-81221541 Msa0577050:CDS 50.0%
CCCTCCATATACACCTGAGA+AGG + chr4_1:81221623-81221642 Msa0577050:CDS 50.0%
ATTCACAGTGATTGGGCCTC+TGG - chr4_1:81221839-81221858 None:intergenic 50.0%
! CAAGCAATGGCGGAAGACAG+AGG - chr4_1:81217651-81217670 None:intergenic 55.0%
TTCCGCCATTGCTTGCTCCT+AGG + chr4_1:81217656-81217675 Msa0577050:CDS 55.0%
CAAAGGCTCTGCTACCAACC+AGG - chr4_1:81217798-81217817 None:intergenic 55.0%
GCGGAAATGGCTCCAATGAC+TGG - chr4_1:81219240-81219259 None:intergenic 55.0%
GTCATTGGAGCCATTTCCGC+GGG + chr4_1:81219240-81219259 Msa0577050:CDS 55.0%
GCAAGCAGGGATGATGTTGC+AGG - chr4_1:81219300-81219319 None:intergenic 55.0%
TGCACTCCTGGACCAGAAGT+GGG + chr4_1:81219389-81219408 Msa0577050:CDS 55.0%
GAGAACGGGTCCTGGTAATC+GGG - chr4_1:81219641-81219660 None:intergenic 55.0%
ACTCCGGTTCCCAAGAGAAC+GGG - chr4_1:81219655-81219674 None:intergenic 55.0%
CACTCCGGTTCCCAAGAGAA+CGG - chr4_1:81219656-81219675 None:intergenic 55.0%
TTGGTGCAGGTTGCAACTCG+TGG + chr4_1:81221474-81221493 Msa0577050:intron 55.0%
CAAAACCACTGCGTTGCCCG+CGG - chr4_1:81219259-81219278 None:intergenic 60.0%
CTGCACTCCTGGACCAGAAG+TGG + chr4_1:81219388-81219407 Msa0577050:CDS 60.0%
GTTCCCAAGAGAACGGGTCC+TGG - chr4_1:81219649-81219668 None:intergenic 60.0%
GGACCCGTTCTCTTGGGAAC+CGG + chr4_1:81219649-81219668 Msa0577050:intron 60.0%
CACTGCGTTGCCCGCGGAAA+TGG - chr4_1:81219253-81219272 None:intergenic 65.0%
CATTTCCGCGGGCAACGCAG+TGG + chr4_1:81219251-81219270 Msa0577050:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 81217361 81221963 81217361 ID=Msa0577050;Name=Msa0577050
chr4_1 mRNA 81217361 81221963 81217361 ID=Msa0577050-mRNA-1;Parent=Msa0577050;Name=Msa0577050-mRNA-1;_AED=0.16;_eAED=0.16;_QI=279|0.90|0.91|1|0.81|0.91|12|244|512
chr4_1 exon 81217361 81217677 81217361 ID=Msa0577050-mRNA-1:exon:29218;Parent=Msa0577050-mRNA-1
chr4_1 exon 81217792 81217893 81217792 ID=Msa0577050-mRNA-1:exon:29219;Parent=Msa0577050-mRNA-1
chr4_1 exon 81217997 81218150 81217997 ID=Msa0577050-mRNA-1:exon:29220;Parent=Msa0577050-mRNA-1
chr4_1 exon 81218661 81218729 81218661 ID=Msa0577050-mRNA-1:exon:29221;Parent=Msa0577050-mRNA-1
chr4_1 exon 81218807 81218903 81218807 ID=Msa0577050-mRNA-1:exon:29222;Parent=Msa0577050-mRNA-1
chr4_1 exon 81219223 81219429 81219223 ID=Msa0577050-mRNA-1:exon:29223;Parent=Msa0577050-mRNA-1
chr4_1 exon 81219927 81220042 81219927 ID=Msa0577050-mRNA-1:exon:29224;Parent=Msa0577050-mRNA-1
chr4_1 exon 81220130 81220234 81220130 ID=Msa0577050-mRNA-1:exon:29225;Parent=Msa0577050-mRNA-1
chr4_1 exon 81220310 81220482 81220310 ID=Msa0577050-mRNA-1:exon:29226;Parent=Msa0577050-mRNA-1
chr4_1 exon 81220571 81220661 81220571 ID=Msa0577050-mRNA-1:exon:29227;Parent=Msa0577050-mRNA-1
chr4_1 exon 81220751 81220900 81220751 ID=Msa0577050-mRNA-1:exon:29228;Parent=Msa0577050-mRNA-1
chr4_1 exon 81221483 81221963 81221483 ID=Msa0577050-mRNA-1:exon:29229;Parent=Msa0577050-mRNA-1
chr4_1 five_prime_UTR 81217361 81217639 81217361 ID=Msa0577050-mRNA-1:five_prime_utr;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81217640 81217677 81217640 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81217792 81217893 81217792 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81217997 81218150 81217997 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81218661 81218729 81218661 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81218807 81218903 81218807 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81219223 81219429 81219223 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81219927 81220042 81219927 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81220130 81220234 81220130 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81220310 81220482 81220310 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81220571 81220661 81220571 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81220751 81220900 81220751 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 CDS 81221483 81221719 81221483 ID=Msa0577050-mRNA-1:cds;Parent=Msa0577050-mRNA-1
chr4_1 three_prime_UTR 81221720 81221963 81221720 ID=Msa0577050-mRNA-1:three_prime_utr;Parent=Msa0577050-mRNA-1
Gene Sequence

>Msa0577050

ATGAAAAGCCTCTGTCTTCCGCCATTGCTTGCTCCTAGTGCCCTGGTTGGTAGCAGAGCCTTTGCAAACAATTTAACAACAAAGTGCTTTCCCAAGCAACTTCATTTCAACTCTCGCTCTGTTGTTTTTTCTTCACACTTGTGTTATGCAACTCTATCTGTGGAATCAGAAGTAGAAAATAAACAAGTATTTGATGGAGAGAAAGCTTCTTTGCTTGTTAGAGAGCTCAGGAAGAATTTTGACACTGGCAAGACAAAGAGCTACGAATGGCGGATTTCCCAATTGGAGGCCATAATTGTCCAGGCGAAATCTTCATGTGATGAAGCACTTCAAGAACTGAAACACTGGATGAAACCTGAAAAGGTGAGTACTTCAATCACAGCATTTCCATCATCAGCAGAGATTGTGTCCGAACCACTAGGAGTTGTGTTGATCATATCAACATGGAACTTTCCTATGTTGTTATCATTGGATCCAGTCATTGGAGCCATTTCCGCGGGCAACGCAGTGGTTTTGAAACCATCAGAAGTTTCTCCTGCAACATCATCCCTGCTTGCAAATTTGCTAGAAAGTTATCTAGACAACTCTGCAGTAAGAGTTGTTGAAGGGGCTGTTCCTGAAACAACTGCACTCCTGGACCAGAAGTGGGATAAGATACTTTACACAGGTAGTGCTAGAGTAGGTCGCATTGTTATGGCTGCTGCTGCGAAGCATCTTACTCCAGTGATTCTTGAGCTTGGAGGAAAGTGTCCAGCTGTTGTTGATTCAAATGTCAACTTACAATTCACTGCGAGGAGGATAATAGCTGGCAAATGGGCATGCAATAGTGGACAAACATGCATTTCTGTTGATTATATCATCACAACAAAAGACTTCGCTCCAACGCTTATAGCAGCTTTAAAGGAAGAATTGGAGCAATTTTTTGGGAAAGATCTGATGGAATCGAAAGACATGTCTCGTATTGTGTCTCCAACTCAATATGCACGACTCGTGAAACTCTTGAATGAGGATAAGGTATCAGACAAAATTGTTTTAGGAGGTCAGAGGGATGAGAAGAAATTAAGGATTGCTCCAACTATCTTATTGGATGTTCCACAAGAAGCTATGGTTATGCAAGAGGAGATATTTGGGCCAATTTTGCCAATTATTACTGTAGACAACATTGAAGATAGCTTTAGTATAATAAAATCAAGGCCAAAGCCTTTGGCTGCATATCTCTTCACAAACAATGAGCAACTGAAGAAGGATTATGTGGACAAGATATCTTCTGGAGGGATGCTCATCAACGACGCTGTCATACATGTTGCAACTCGTGGTTTGCCTTTTGGAGGAGTTGGAGAAAGTGGAATGGGTTGCTACCATGGAAAGTTCTCTTTTGATAGTTTCAGCCATAAGAAATCAGTTTTATATAAAACTTTTGATTCCGATCCATCCATAAGGTACCCTCCATATACACCTGAGAAGGAAAAAATTTTGAGGGCCATTTTTGGTGGCAATATTTTTCAGATAATTCTTGCTTTGCTTGGATGGTCTAAATGA
Protein sequence

>Msa0577050

MKSLCLPPLLAPSALVGSRAFANNLTTKCFPKQLHFNSRSVVFSSHLCYATLSVESEVENKQVFDGEKASLLVRELRKNFDTGKTKSYEWRISQLEAIIVQAKSSCDEALQELKHWMKPEKVSTSITAFPSSAEIVSEPLGVVLIISTWNFPMLLSLDPVIGAISAGNAVVLKPSEVSPATSSLLANLLESYLDNSAVRVVEGAVPETTALLDQKWDKILYTGSARVGRIVMAAAAKHLTPVILELGGKCPAVVDSNVNLQFTARRIIAGKWACNSGQTCISVDYIITTKDFAPTLIAALKEELEQFFGKDLMESKDMSRIVSPTQYARLVKLLNEDKVSDKIVLGGQRDEKKLRIAPTILLDVPQEAMVMQEEIFGPILPIITVDNIEDSFSIIKSRPKPLAAYLFTNNEQLKKDYVDKISSGGMLINDAVIHVATRGLPFGGVGESGMGCYHGKFSFDSFSHKKSVLYKTFDSDPSIRYPPYTPEKEKILRAIFGGNIFQIILALLGWSK*