AlfalfaGEDB | Msa1244650

M. sativa cultivar zhongmu-4 / Msa1244650

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010321 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0048580 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000026 GO:2000112 GO:2001141
PFAM: PF03110.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1244650	XP_013445497.1	98.889	180	2	0	1	180	1	180	5.59e-127	364

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1244650	sp\|Q38740\|SBP2_ANTMA	69.474	95	29	0	55	149	75	169	2.65e-43	145

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification
Msa1244650	TF	SBP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008330	Msa1244650	0.812174	4.841680e-51	-8.615850e-47
Msa0053960	Msa1244650	0.802358	5.827284e-49	-8.615850e-47
Msa0117300	Msa1244650	0.803149	4.000888e-49	-8.615850e-47
Msa0608940	Msa1244650	0.800924	1.147013e-48	-8.615850e-47
Msa0618260	Msa1244650	0.822074	2.877946e-53	-8.615850e-47
Msa0646390	Msa1244650	0.806048	9.938586e-50	-8.615850e-47
Msa0657860	Msa1244650	0.807010	6.227261e-50	-8.615850e-47
Msa0726610	Msa1244650	0.812722	3.674377e-51	-8.615850e-47
Msa0727300	Msa1244650	0.817204	3.722272e-52	-8.615850e-47
Msa0738600	Msa1244650	0.806186	9.294082e-50	-8.615850e-47
Msa0748980	Msa1244650	0.810984	8.782488e-51	-8.615850e-47
Msa0752830	Msa1244650	0.806834	6.785715e-50	-8.615850e-47
Msa0756940	Msa1244650	0.821464	3.981775e-53	-8.615850e-47
Msa0795400	Msa1244650	0.802872	4.565010e-49	-8.615850e-47
Msa0818950	Msa1244650	0.811128	8.172345e-51	-8.615850e-47
Msa0825520	Msa1244650	0.802951	4.396569e-49	-8.615850e-47
Msa0836510	Msa1244650	0.818542	1.856287e-52	-8.615850e-47
Msa0133450	Msa1244650	0.800621	1.322369e-48	-8.615850e-47
Msa0143360	Msa1244650	0.800079	1.705396e-48	-8.615850e-47
Msa0148430	Msa1244650	0.805526	1.279560e-49	-8.615850e-47
Msa0164630	Msa1244650	0.819524	1.109881e-52	-8.615850e-47
Msa0221940	Msa1244650	0.802665	5.037311e-49	-8.615850e-47
Msa1244650	Msa1293580	0.926142	7.032097e-91	-8.615850e-47
Msa1244650	Msa1296610	0.803434	3.492948e-49	-8.615850e-47
Msa1244650	Msa1329820	0.813874	2.052132e-51	-8.615850e-47
Msa1244650	Msa1349640	0.806026	1.004343e-49	-8.615850e-47
Msa1244650	Msa1357380	0.816492	5.377913e-52	-8.615850e-47
Msa1244650	Msa1366700	0.807866	4.099266e-50	-8.615850e-47
Msa1244650	Msa1368660	0.817806	2.723844e-52	-8.615850e-47
Msa1244650	Msa1381720	0.808423	3.120426e-50	-8.615850e-47
Msa1244650	Msa1384620	0.805397	1.361551e-49	-8.615850e-47
Msa1244650	Msa1391600	0.814362	1.601282e-51	-8.615850e-47
Msa1244650	Msa1395580	0.834966	2.207549e-56	-8.615850e-47
Msa1244650	Msa1397540	0.828606	8.188682e-55	-8.615850e-47
Msa1244650	Msa1413880	0.815852	7.474846e-52	-8.615850e-47
Msa1244650	Msa1440160	0.812051	5.149630e-51	-8.615850e-47
Msa1244650	Msa1446220	0.801173	1.020434e-48	-8.615850e-47
Msa1244650	Msa1450850	0.803076	4.141631e-49	-8.615850e-47
Msa1244650	Msa1459630	0.802768	4.795836e-49	-8.615850e-47
Msa0373110	Msa1244650	0.817320	3.505311e-52	-8.615850e-47
Msa0402640	Msa1244650	0.801455	8.933320e-49	-8.615850e-47
Msa0427050	Msa1244650	0.803290	3.740083e-49	-8.615850e-47
Msa0431940	Msa1244650	0.823887	1.087410e-53	-8.615850e-47
Msa0467480	Msa1244650	0.840189	1.009863e-57	-8.615850e-47
Msa1008960	Msa1244650	0.816149	6.416867e-52	-8.615850e-47
Msa1018720	Msa1244650	0.813031	3.145040e-51	-8.615850e-47
Msa1024120	Msa1244650	0.802795	4.734452e-49	-8.615850e-47
Msa1030430	Msa1244650	0.819745	9.882706e-53	-8.615850e-47
Msa1050320	Msa1244650	0.813126	2.996297e-51	-8.615850e-47
Msa1067910	Msa1244650	0.837606	4.708014e-57	-8.615850e-47
Msa1071310	Msa1244650	0.806061	9.874386e-50	-8.615850e-47
Msa1071460	Msa1244650	0.804152	2.478067e-49	-8.615850e-47
Msa0338610	Msa1244650	0.800849	1.188282e-48	-8.615850e-47
Msa0343200	Msa1244650	0.817486	3.216859e-52	-8.615850e-47
Msa1107950	Msa1244650	0.809135	2.197672e-50	-8.615850e-47
Msa1113210	Msa1244650	0.867512	1.295604e-65	-8.615850e-47
Msa1125100	Msa1244650	0.806189	9.283121e-50	-8.615850e-47
Msa1125150	Msa1244650	0.803687	3.095245e-49	-8.615850e-47
Msa1186010	Msa1244650	0.810868	9.305077e-51	-8.615850e-47
Msa1190980	Msa1244650	0.860721	1.695658e-63	-8.615850e-47
Msa1208250	Msa1244650	0.812925	3.317416e-51	-8.615850e-47
Msa1211280	Msa1244650	0.804313	2.293332e-49	-8.615850e-47
Msa1211370	Msa1244650	0.837576	4.789928e-57	-8.615850e-47
Msa0520810	Msa1244650	0.803357	3.622675e-49	-8.615850e-47
Msa0566010	Msa1244650	0.813938	1.986645e-51	-8.615850e-47
Msa0584590	Msa1244650	0.826525	2.585898e-54	-8.615850e-47
Msa0861480	Msa1244650	0.810922	9.057294e-51	-8.615850e-47
Msa0864730	Msa1244650	0.802694	4.968864e-49	-8.615850e-47
Msa0864750	Msa1244650	0.802455	5.563961e-49	-8.615850e-47
Msa0880060	Msa1244650	0.801383	9.241834e-49	-8.615850e-47
Msa0887000	Msa1244650	0.804532	2.065106e-49	-8.615850e-47
Msa0927890	Msa1244650	0.800364	1.492040e-48	-8.615850e-47
Msa0961230	Msa1244650	0.808405	3.147103e-50	-8.615850e-47
Msa0967260	Msa1244650	0.816128	6.487390e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1244650	MtrunA17_Chr8g0360361	98.889	180	2	0	1	180	1	180	5.14e-131	364
Msa1244650	MtrunA17_Chr4g0046991	68.132	91	29	0	56	146	64	154	4.44e-43	140
Msa1244650	MtrunA17_Chr7g0270541	53.061	147	50	2	15	149	90	229	1.88e-38	139
Msa1244650	MtrunA17_Chr7g0258471	67.778	90	26	1	57	143	131	220	1.78e-37	133
Msa1244650	MtrunA17_Chr8g0334671	67.010	97	31	1	55	150	177	273	2.25e-37	131
Msa1244650	MtrunA17_Chr2g0282521	65.116	86	30	0	57	142	56	141	5.86e-37	125
Msa1244650	MtrunA17_Chr3g0122411	68.293	82	26	0	63	144	163	244	6.36e-37	132
Msa1244650	MtrunA17_Chr1g0194121	54.348	138	56	2	15	148	96	230	2.57e-36	133
Msa1244650	MtrunA17_Chr7g0231911	64.516	93	33	0	57	149	141	233	4.53e-36	127
Msa1244650	MtrunA17_Chr1g0162871	62.887	97	36	0	53	149	92	188	1.90e-34	128
Msa1244650	MtrunA17_Chr2g0302791	72.500	80	22	0	65	144	139	218	2.26e-34	127
Msa1244650	MtrunA17_Chr7g0224821	63.291	79	29	0	65	143	77	155	1.96e-32	119
Msa1244650	MtrunA17_Chr4g0061651	65.476	84	28	1	65	147	123	206	3.73e-32	120
Msa1244650	MtrunA17_Chr3g0132091	64.103	78	28	0	65	142	73	150	4.19e-32	118
Msa1244650	MtrunA17_Chr5g0420051	67.105	76	25	0	63	138	179	254	2.76e-31	117
Msa1244650	MtrunA17_Chr8g0386271	57.292	96	41	0	54	149	88	183	3.10e-31	116
Msa1244650	MtrunA17_Chr8g0377401	64.474	76	27	0	62	137	159	234	7.51e-31	112
Msa1244650	MtrunA17_Chr2g0308321	68.919	74	23	0	65	138	171	244	2.88e-30	115
Msa1244650	MtrunA17_Chr8g0377421	62.338	77	29	0	62	138	129	205	3.75e-30	112
Msa1244650	MtrunA17_Chr2g0317041	72.857	70	16	2	45	111	76	145	1.07e-26	99.0
Msa1244650	MtrunA17_Chr2g0286661	42.857	98	56	0	65	162	103	200	1.60e-20	88.2
Msa1244650	MtrunA17_Chr1g0175031	66.000	50	17	0	62	111	71	120	3.56e-17	73.9
Msa1244650	MtrunA17_Chr8g0377431	56.897	58	25	0	63	120	149	206	3.51e-16	73.2
Msa1244650	MtrunA17_Chr2g0317031	55.072	69	29	1	112	180	9	75	9.20e-16	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1244650	AT3G15270.1	48.913	184	87	5	1	180	1	181	1.63e-42	140
Msa1244650	AT3G60030.1	50.993	151	69	1	6	151	63	213	1.78e-40	145
Msa1244650	AT1G53160.2	54.331	127	53	2	59	180	48	174	5.15e-40	134
Msa1244650	AT1G53160.1	54.331	127	53	2	59	180	48	174	5.15e-40	134
Msa1244650	AT1G53160.3	69.231	91	27	1	59	148	48	138	6.32e-39	131
Msa1244650	AT2G33810.1	65.517	87	30	0	56	142	45	131	1.48e-38	129
Msa1244650	AT1G02065.1	70.115	87	26	0	52	138	175	261	1.18e-36	130
Msa1244650	AT2G47070.1	56.034	116	47	1	33	148	78	189	6.45e-36	132
Msa1244650	AT2G42200.1	70.370	81	24	0	63	143	72	152	2.57e-34	124
Msa1244650	AT1G69170.1	69.737	76	23	0	63	138	122	197	5.97e-33	121
Msa1244650	AT1G20980.1	64.211	95	33	1	54	147	109	203	8.35e-33	123
Msa1244650	AT5G43270.3	64.706	85	30	0	63	147	167	251	1.88e-32	120
Msa1244650	AT5G43270.1	64.706	85	30	0	63	147	167	251	1.88e-32	120
Msa1244650	AT5G43270.2	64.706	85	30	0	63	147	167	251	1.88e-32	120
Msa1244650	AT5G50570.1	62.500	80	30	0	63	142	99	178	6.08e-32	118
Msa1244650	AT5G50570.2	62.500	80	30	0	63	142	99	178	6.08e-32	118
Msa1244650	AT5G50670.1	62.500	80	30	0	63	142	99	178	6.08e-32	118
Msa1244650	AT5G50670.2	62.500	80	30	0	63	142	99	178	6.08e-32	118
Msa1244650	AT3G57920.1	62.500	88	33	0	65	152	59	146	1.51e-31	117
Msa1244650	AT1G76580.2	68.750	80	25	0	63	142	112	191	1.66e-31	119
Msa1244650	AT1G76580.3	68.750	80	25	0	63	142	112	191	1.88e-31	119
Msa1244650	AT1G76580.1	68.750	80	25	0	63	142	112	191	2.78e-31	119
Msa1244650	AT1G27360.2	51.786	112	48	1	49	154	159	270	9.55e-30	112
Msa1244650	AT1G27360.1	51.786	112	48	1	49	154	159	270	9.55e-30	112
Msa1244650	AT1G27360.3	51.786	112	48	1	49	154	159	270	9.55e-30	112
Msa1244650	AT1G27360.4	51.786	112	48	1	49	154	159	270	9.55e-30	112
Msa1244650	AT1G27370.6	60.976	82	32	0	57	138	168	249	1.00e-29	112
Msa1244650	AT1G27370.1	60.976	82	32	0	57	138	168	249	2.84e-29	111
Msa1244650	AT1G27370.5	60.976	82	32	0	57	138	168	249	2.84e-29	111
Msa1244650	AT1G27370.3	60.976	82	32	0	57	138	168	249	2.84e-29	111
Msa1244650	AT1G27370.2	60.976	82	32	0	57	138	168	249	2.84e-29	111
Msa1244650	AT1G27370.4	60.976	82	32	0	57	138	168	249	2.84e-29	111
Msa1244650	AT1G27370.7	60.976	82	32	0	57	138	168	249	2.84e-29	111
Msa1244650	AT1G02065.2	68.852	61	19	0	52	112	175	235	1.59e-23	93.6
Msa1244650	AT2G47070.2	72.881	59	16	0	90	148	2	60	3.42e-23	96.3
Msa1244650	AT5G18830.1	42.017	119	62	2	20	138	100	211	8.79e-21	89.4
Msa1244650	AT5G18830.3	42.017	119	62	2	20	138	100	211	8.90e-21	89.4
Msa1244650	AT5G18830.2	42.017	119	62	2	20	138	100	211	1.02e-20	89.0

Find 86 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATGAAACTCACACACTTTA+TGG	0.102129	8_2:+33586885	None:intergenic
CTTCCCTCATTGGCAGTTTC+CGG	0.104015	8_2:+33586160	None:intergenic
CTCTCTCTTCTGTCACCTTC+TGG	0.186522	8_2:-33585996	Msa1244650:three_prime_UTR
CCATTTAGATGTAGGATATT+TGG	0.207975	8_2:+33585904	None:intergenic
AGGATATTTGGTGGGTAATT+AGG	0.244632	8_2:+33585916	None:intergenic
CATTCAAAGGTGCTTTAATA+AGG	0.264972	8_2:+33585835	None:intergenic
TTCCCTCATTGGCAGTTTCC+GGG	0.286723	8_2:+33586161	None:intergenic
AATAGAAGAAGCTTGAGTTT+TGG	0.300418	8_2:+33587217	None:intergenic
GTGAACTGACAATGGCTATA+AGG	0.313347	8_2:+33587169	None:intergenic
GCACCTGTTGTGGTGGTTGC+AGG	0.325472	8_2:-33586857	Msa1244650:CDS
GCTGTGACTGCTTCCCTCAT+TGG	0.328447	8_2:+33586150	None:intergenic
ATGAACTTACCAGGCAGTTC+TGG	0.332336	8_2:-33586085	Msa1244650:CDS
CCAAATATCCTACATCTAAA+TGG	0.346083	8_2:-33585904	Msa1244650:three_prime_UTR
GGGGTCGGTGGCGGGAGAAG+AGG	0.366575	8_2:-33586992	Msa1244650:CDS
CTGAAGAAGAGAAGAAGAAA+GGG	0.366865	8_2:-33587012	Msa1244650:CDS
AGCTGCCGCAGGCGGTTGGC+TGG	0.389734	8_2:-33586211	Msa1244650:CDS
TTAGCTTCATCTAATGTTGA+AGG	0.400420	8_2:+33586054	None:intergenic
CAACATGGTGGAAGAAGAAA+TGG	0.403464	8_2:-33587074	Msa1244650:CDS
ATCCCTGCAACCACCACAAC+AGG	0.415205	8_2:+33586854	None:intergenic
AGAAGAAAGGGGTCGGTGGC+GGG	0.418165	8_2:-33587000	Msa1244650:CDS
TGTCACCTTCTGGTTTCTTA+CGG	0.423252	8_2:-33585986	Msa1244650:three_prime_UTR
GTCGGTGGCGGGAGAAGAGG+AGG	0.431374	8_2:-33586989	Msa1244650:CDS
AAGAAGAAAGGGGTCGGTGG+CGG	0.439947	8_2:-33587001	Msa1244650:CDS
TGAACTGACAATGGCTATAA+GGG	0.440303	8_2:+33587170	None:intergenic
CTCATTGGCAGTTTCCGGGT+TGG	0.441178	8_2:+33586165	None:intergenic
ATCCTGCATTGAGTTTCCTT+AGG	0.443576	8_2:+33586112	None:intergenic
GGCGGGAGAAGAGGAGGTGG+TGG	0.448247	8_2:-33586983	Msa1244650:CDS
AGGATCTTTATTCTATGCCT+TGG	0.454275	8_2:+33585962	None:intergenic
AAATGAAGTAGAAGAGGAGG+AGG	0.457054	8_2:-33587050	Msa1244650:CDS
GGGAGAAGAGGAGGTGGTGG+CGG	0.468248	8_2:-33586980	Msa1244650:CDS
TGGTTGCAGGGATGAGGCAG+AGG	0.476673	8_2:-33586844	Msa1244650:CDS
ACTGAAGAAGAGAAGAAGAA+AGG	0.477392	8_2:-33587013	Msa1244650:CDS
TTCTGGTTTCTTACGGTCCA+AGG	0.480571	8_2:-33585979	Msa1244650:three_prime_UTR
TCATTCCAAGGCACCTGTTG+TGG	0.481858	8_2:-33586867	Msa1244650:CDS
CACCTCTCTGCTTGGCAACA+AGG	0.490126	8_2:+33586947	None:intergenic
ACTTATGACCATTTAGATGT+AGG	0.499065	8_2:+33585896	None:intergenic
CAGCTCCACACCTCTCTGCT+TGG	0.503571	8_2:+33586939	None:intergenic
AATGGAAAATGAAGTAGAAG+AGG	0.504373	8_2:-33587056	Msa1244650:CDS
TTGTGGTGGTTGCAGGGATG+AGG	0.505992	8_2:-33586850	Msa1244650:CDS
AACCACCACAACAGGTGCCT+TGG	0.507647	8_2:+33586862	None:intergenic
AAGAAGCTGCCGCAGGCGGT+TGG	0.518296	8_2:-33586215	Msa1244650:CDS
GGAAAATGAAGTAGAAGAGG+AGG	0.522072	8_2:-33587053	Msa1244650:CDS
GAGGAAGAGAACATTGATGA+TGG	0.526926	8_2:-33587113	Msa1244650:CDS
AATAGGGTTACAACATTCAA+AGG	0.527736	8_2:+33585822	None:intergenic
TTTAGATGTAGGATATTTGG+TGG	0.531466	8_2:+33585907	None:intergenic
GGTGGCGGGAGAAGAGGAGG+TGG	0.533572	8_2:-33586986	Msa1244650:CDS
CACACACTTTATGGCGGCGA+TGG	0.534636	8_2:+33586894	None:intergenic
GCTGTGCAGGTTCCATGACT+TGG	0.541073	8_2:-33586257	Msa1244650:intron
GAAGAGAAGAAGAAAGGGGT+CGG	0.545505	8_2:-33587007	Msa1244650:CDS
CACCTGTTGTGGTGGTTGCA+GGG	0.546200	8_2:-33586856	Msa1244650:CDS
TTCCAAGGCACCTGTTGTGG+TGG	0.554919	8_2:-33586864	Msa1244650:CDS
CTCACAAAACAATGGAAGCT+AGG	0.561345	8_2:-33587144	Msa1244650:exon
AGTGTGTGAGTTTCATTCCA+AGG	0.564413	8_2:-33586879	Msa1244650:CDS
CAACCCGGAAACTGCCAATG+AGG	0.564468	8_2:-33586164	Msa1244650:CDS
GACTTATAACCAGAACTGCC+TGG	0.566906	8_2:+33586076	None:intergenic
GGTGACAGAAGAGAGAGAGC+TGG	0.567068	8_2:+33586002	None:intergenic
CAAAGGACTACATCAACCTA+AGG	0.568949	8_2:-33586128	Msa1244650:CDS
CATCAAACTCTACCAAGTCA+TGG	0.569400	8_2:+33586245	None:intergenic
AACCTAAGGAAACTCAATGC+AGG	0.572114	8_2:-33586114	Msa1244650:CDS
TGATGATGGAAAAGATGAGA+AGG	0.573354	8_2:-33587099	Msa1244650:CDS
CTGGACACAATGAACGGCGC+CGG	0.573671	8_2:-33586192	Msa1244650:CDS
TGTGTCCAGCCAACCGCCTG+CGG	0.577001	8_2:+33586206	None:intergenic
GAGGTGTGGAGCTGATTTGA+CGG	0.578847	8_2:-33586930	Msa1244650:CDS
GAGAAGAAGAAAGGGGTCGG+TGG	0.581205	8_2:-33587004	Msa1244650:CDS
ACGGCGCCGGAAAACCAACC+CGG	0.589104	8_2:-33586179	Msa1244650:CDS
TTGTTGTGATGAACATACAA+AGG	0.601220	8_2:+33585942	None:intergenic
AGTCTAAAAGAAGCTGCCGC+AGG	0.602245	8_2:-33586222	Msa1244650:CDS
AACCCGGAAACTGCCAATGA+GGG	0.603733	8_2:-33586163	Msa1244650:CDS
AATGAAGTAGAAGAGGAGGA+GGG	0.609345	8_2:-33587049	Msa1244650:CDS
AGGATTCAGATGAACTTACC+AGG	0.609388	8_2:-33586094	Msa1244650:CDS
CTAAAAGAAGCTGCCGCAGG+CGG	0.624624	8_2:-33586219	Msa1244650:CDS
TGTTGCCAAGCAGAGAGGTG+TGG	0.631386	8_2:-33586944	Msa1244650:CDS
GTTCACCTCTCACAAAACAA+TGG	0.632717	8_2:-33587152	Msa1244650:exon
GAGGGAAGCAGTCACAGCAA+AGG	0.634063	8_2:-33586145	Msa1244650:CDS
TGTGAGAGGTGAACTGACAA+TGG	0.637177	8_2:+33587161	None:intergenic
TTAGATGTAGGATATTTGGT+GGG	0.641473	8_2:+33585908	None:intergenic
CACCTTGTTGCCAAGCAGAG+AGG	0.641872	8_2:-33586949	Msa1244650:CDS
GGTTGGCTGGACACAATGAA+CGG	0.643542	8_2:-33586198	Msa1244650:CDS
CTCTCTGCTTGGCAACAAGG+TGG	0.645735	8_2:+33586950	None:intergenic
TTGGACCGTAAGAAACCAGA+AGG	0.656890	8_2:+33585981	None:intergenic
TGAAGAAGAGAAGAAGAAAG+GGG	0.662619	8_2:-33587011	Msa1244650:CDS
TGGAAGCTAGGAGTATTGAG+AGG	0.681882	8_2:-33587132	Msa1244650:CDS
ACTGACAATGGCTATAAGGG+AGG	0.683768	8_2:+33587173	None:intergenic
GAAACTCACACACTTTATGG+CGG	0.694059	8_2:+33586888	None:intergenic
GATGAGAAGGAACAACATGG+TGG	0.695331	8_2:-33587086	Msa1244650:CDS
AAAGATGAGAAGGAACAACA+TGG	0.732079	8_2:-33587089	Msa1244650:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGCAAACAAAAATAATTA+AGG	-	chr8_2:33586409-33586428	Msa1244650:intron	15.0%
!!	AAGCAAACAAAAATAATTAA+GGG	-	chr8_2:33586410-33586429	Msa1244650:intron	15.0%
!!	AAAGCAAACAAAAATAATTA+AGG	-	chr8_2:33586409-33586428	Msa1244650:intron	15.0%
!!	AAGCAAACAAAAATAATTAA+GGG	-	chr8_2:33586410-33586429	Msa1244650:intron	15.0%
!!!	TTTTCTTTTGACATGTTTAT+AGG	-	chr8_2:33586254-33586273	Msa1244650:intron	20.0%
!!	AAATCGAATGTTTCAAAAAT+TGG	-	chr8_2:33586332-33586351	Msa1244650:intron	20.0%
!!	GATTAAGTTATACAATAGTT+CGG	+	chr8_2:33586480-33586499	None:intergenic	20.0%
!!!	TATTCTTTTTGCAGAAATTT+AGG	+	chr8_2:33586663-33586682	None:intergenic	20.0%
!!!	TTTTCTTTTGACATGTTTAT+AGG	-	chr8_2:33586254-33586273	Msa1244650:intron	20.0%
!!	AAATCGAATGTTTCAAAAAT+TGG	-	chr8_2:33586332-33586351	Msa1244650:intron	20.0%
!!	GATTAAGTTATACAATAGTT+CGG	+	chr8_2:33586480-33586499	None:intergenic	20.0%
!!!	TATTCTTTTTGCAGAAATTT+AGG	+	chr8_2:33586663-33586682	None:intergenic	20.0%
!	CAAACAAAAATAATTAAGGG+AGG	-	chr8_2:33586413-33586432	Msa1244650:intron	25.0%
!	ACTATTGTATAACTTAATCC+CGG	-	chr8_2:33586481-33586500	Msa1244650:intron	25.0%
!!!	TTGAAGCAATGTTTTAAAAC+CGG	+	chr8_2:33586554-33586573	None:intergenic	25.0%
!	AAAACTGACCTTAAATATCT+TGG	-	chr8_2:33586616-33586635	Msa1244650:intron	25.0%
!	TCCAAAAGAATAAAAAGCTA+CGG	-	chr8_2:33587209-33587228	Msa1244650:five_prime_UTR	25.0%
!	CAAACAAAAATAATTAAGGG+AGG	-	chr8_2:33586413-33586432	Msa1244650:intron	25.0%
!	ACTATTGTATAACTTAATCC+CGG	-	chr8_2:33586481-33586500	Msa1244650:intron	25.0%
!!!	TTGAAGCAATGTTTTAAAAC+CGG	+	chr8_2:33586554-33586573	None:intergenic	25.0%
!	AAAACTGACCTTAAATATCT+TGG	-	chr8_2:33586616-33586635	Msa1244650:intron	25.0%
!	TCCAAAAGAATAAAAAGCTA+CGG	-	chr8_2:33587209-33587228	Msa1244650:five_prime_UTR	25.0%
!	ATAGAAGAAGCTTGAGTTTT+GGG	+	chr8_2:33585787-33585806	None:intergenic	30.0%
!	AATAGAAGAAGCTTGAGTTT+TGG	+	chr8_2:33585788-33585807	None:intergenic	30.0%
	AATGGAAAATGAAGTAGAAG+AGG	-	chr8_2:33585946-33585965	Msa1244650:three_prime_UTR	30.0%
	AATGAAACTCACACACTTTA+TGG	+	chr8_2:33586120-33586139	None:intergenic	30.0%
	TTGATGAGTGTTAGAGAAAA+TGG	+	chr8_2:33586225-33586244	None:intergenic	30.0%
!	TCTTTTGACATGTTTATAGG+TGG	-	chr8_2:33586257-33586276	Msa1244650:intron	30.0%
	GAGCTAAAAAAACAGATCAT+GGG	+	chr8_2:33586301-33586320	None:intergenic	30.0%
	TGAGCTAAAAAAACAGATCA+TGG	+	chr8_2:33586302-33586321	None:intergenic	30.0%
	TTAGCTTCATCTAATGTTGA+AGG	+	chr8_2:33586951-33586970	None:intergenic	30.0%
	TTGTTGTGATGAACATACAA+AGG	+	chr8_2:33587063-33587082	None:intergenic	30.0%
	TTAGATGTAGGATATTTGGT+GGG	+	chr8_2:33587097-33587116	None:intergenic	30.0%
	TTTAGATGTAGGATATTTGG+TGG	+	chr8_2:33587098-33587117	None:intergenic	30.0%
	CCATTTAGATGTAGGATATT+TGG	+	chr8_2:33587101-33587120	None:intergenic	30.0%
	CCAAATATCCTACATCTAAA+TGG	-	chr8_2:33587098-33587117	Msa1244650:CDS	30.0%
	ACTTATGACCATTTAGATGT+AGG	+	chr8_2:33587109-33587128	None:intergenic	30.0%
!!	CATTCAAAGGTGCTTTAATA+AGG	+	chr8_2:33587170-33587189	None:intergenic	30.0%
	AATAGGGTTACAACATTCAA+AGG	+	chr8_2:33587183-33587202	None:intergenic	30.0%
!!!	TTCTTTTGGAGTGGAAAATA+GGG	+	chr8_2:33587199-33587218	None:intergenic	30.0%
!!!	ATTCTTTTGGAGTGGAAAAT+AGG	+	chr8_2:33587200-33587219	None:intergenic	30.0%
!!	AGCTTTTTATTCTTTTGGAG+TGG	+	chr8_2:33587208-33587227	None:intergenic	30.0%
!	ATAGAAGAAGCTTGAGTTTT+GGG	+	chr8_2:33585787-33585806	None:intergenic	30.0%
!	AATAGAAGAAGCTTGAGTTT+TGG	+	chr8_2:33585788-33585807	None:intergenic	30.0%
	AATGGAAAATGAAGTAGAAG+AGG	-	chr8_2:33585946-33585965	Msa1244650:three_prime_UTR	30.0%
	AATGAAACTCACACACTTTA+TGG	+	chr8_2:33586120-33586139	None:intergenic	30.0%
	TTGATGAGTGTTAGAGAAAA+TGG	+	chr8_2:33586225-33586244	None:intergenic	30.0%
!	TCTTTTGACATGTTTATAGG+TGG	-	chr8_2:33586257-33586276	Msa1244650:intron	30.0%
	GAGCTAAAAAAACAGATCAT+GGG	+	chr8_2:33586301-33586320	None:intergenic	30.0%
	TGAGCTAAAAAAACAGATCA+TGG	+	chr8_2:33586302-33586321	None:intergenic	30.0%
	TTAGCTTCATCTAATGTTGA+AGG	+	chr8_2:33586951-33586970	None:intergenic	30.0%
	TTGTTGTGATGAACATACAA+AGG	+	chr8_2:33587063-33587082	None:intergenic	30.0%
	TTAGATGTAGGATATTTGGT+GGG	+	chr8_2:33587097-33587116	None:intergenic	30.0%
	TTTAGATGTAGGATATTTGG+TGG	+	chr8_2:33587098-33587117	None:intergenic	30.0%
	CCATTTAGATGTAGGATATT+TGG	+	chr8_2:33587101-33587120	None:intergenic	30.0%
	CCAAATATCCTACATCTAAA+TGG	-	chr8_2:33587098-33587117	Msa1244650:CDS	30.0%
	ACTTATGACCATTTAGATGT+AGG	+	chr8_2:33587109-33587128	None:intergenic	30.0%
!!	CATTCAAAGGTGCTTTAATA+AGG	+	chr8_2:33587170-33587189	None:intergenic	30.0%
	AATAGGGTTACAACATTCAA+AGG	+	chr8_2:33587183-33587202	None:intergenic	30.0%
!!!	TTCTTTTGGAGTGGAAAATA+GGG	+	chr8_2:33587199-33587218	None:intergenic	30.0%
!!!	ATTCTTTTGGAGTGGAAAAT+AGG	+	chr8_2:33587200-33587219	None:intergenic	30.0%
!!	AGCTTTTTATTCTTTTGGAG+TGG	+	chr8_2:33587208-33587227	None:intergenic	30.0%
	TGAACTGACAATGGCTATAA+GGG	+	chr8_2:33585835-33585854	None:intergenic	35.0%
!!	TGATGATGGAAAAGATGAGA+AGG	-	chr8_2:33585903-33585922	Msa1244650:three_prime_UTR	35.0%
	AAAGATGAGAAGGAACAACA+TGG	-	chr8_2:33585913-33585932	Msa1244650:three_prime_UTR	35.0%
	ACTGAAGAAGAGAAGAAGAA+AGG	-	chr8_2:33585989-33586008	Msa1244650:three_prime_UTR	35.0%
	CTGAAGAAGAGAAGAAGAAA+GGG	-	chr8_2:33585990-33586009	Msa1244650:three_prime_UTR	35.0%
	TGAAGAAGAGAAGAAGAAAG+GGG	-	chr8_2:33585991-33586010	Msa1244650:three_prime_UTR	35.0%
	TATTAGACTGTGAGACCTTA+AGG	-	chr8_2:33586361-33586380	Msa1244650:intron	35.0%
	CCAAGTAAACGATAACCTTA+AGG	+	chr8_2:33586379-33586398	None:intergenic	35.0%
	CCTTAAGGTTATCGTTTACT+TGG	-	chr8_2:33586376-33586395	Msa1244650:intron	35.0%
	TCCTATTCAACCGATTGTAA+CGG	+	chr8_2:33586522-33586541	None:intergenic	35.0%
	TTGAATAGGACACTTTGATC+CGG	-	chr8_2:33586532-33586551	Msa1244650:intron	35.0%
	GCTCTAGTCCAAGATATTTA+AGG	+	chr8_2:33586627-33586646	None:intergenic	35.0%
	AGGATCTTTATTCTATGCCT+TGG	+	chr8_2:33587043-33587062	None:intergenic	35.0%
!	AGGATATTTGGTGGGTAATT+AGG	+	chr8_2:33587089-33587108	None:intergenic	35.0%
!	GCCGTAGCTTTTTATTCTTT+TGG	+	chr8_2:33587213-33587232	None:intergenic	35.0%
	TGAACTGACAATGGCTATAA+GGG	+	chr8_2:33585835-33585854	None:intergenic	35.0%
!!	TGATGATGGAAAAGATGAGA+AGG	-	chr8_2:33585903-33585922	Msa1244650:three_prime_UTR	35.0%
	AAAGATGAGAAGGAACAACA+TGG	-	chr8_2:33585913-33585932	Msa1244650:three_prime_UTR	35.0%
	ACTGAAGAAGAGAAGAAGAA+AGG	-	chr8_2:33585989-33586008	Msa1244650:three_prime_UTR	35.0%
	CTGAAGAAGAGAAGAAGAAA+GGG	-	chr8_2:33585990-33586009	Msa1244650:three_prime_UTR	35.0%
	TGAAGAAGAGAAGAAGAAAG+GGG	-	chr8_2:33585991-33586010	Msa1244650:three_prime_UTR	35.0%
	TATTAGACTGTGAGACCTTA+AGG	-	chr8_2:33586361-33586380	Msa1244650:intron	35.0%
	CCAAGTAAACGATAACCTTA+AGG	+	chr8_2:33586379-33586398	None:intergenic	35.0%
	CCTTAAGGTTATCGTTTACT+TGG	-	chr8_2:33586376-33586395	Msa1244650:intron	35.0%
	TCCTATTCAACCGATTGTAA+CGG	+	chr8_2:33586522-33586541	None:intergenic	35.0%
	TTGAATAGGACACTTTGATC+CGG	-	chr8_2:33586532-33586551	Msa1244650:intron	35.0%
	GCTCTAGTCCAAGATATTTA+AGG	+	chr8_2:33586627-33586646	None:intergenic	35.0%
	AGGATCTTTATTCTATGCCT+TGG	+	chr8_2:33587043-33587062	None:intergenic	35.0%
!	AGGATATTTGGTGGGTAATT+AGG	+	chr8_2:33587089-33587108	None:intergenic	35.0%
!	GCCGTAGCTTTTTATTCTTT+TGG	+	chr8_2:33587213-33587232	None:intergenic	35.0%
	GTGAACTGACAATGGCTATA+AGG	+	chr8_2:33585836-33585855	None:intergenic	40.0%
	GTTCACCTCTCACAAAACAA+TGG	-	chr8_2:33585850-33585869	Msa1244650:three_prime_UTR	40.0%
!!	AGCTTCCATTGTTTTGTGAG+AGG	+	chr8_2:33585858-33585877	None:intergenic	40.0%
	CTCACAAAACAATGGAAGCT+AGG	-	chr8_2:33585858-33585877	Msa1244650:three_prime_UTR	40.0%
	GAGGAAGAGAACATTGATGA+TGG	-	chr8_2:33585889-33585908	Msa1244650:three_prime_UTR	40.0%
!	CAACATGGTGGAAGAAGAAA+TGG	-	chr8_2:33585928-33585947	Msa1244650:three_prime_UTR	40.0%
	GGAAAATGAAGTAGAAGAGG+AGG	-	chr8_2:33585949-33585968	Msa1244650:three_prime_UTR	40.0%
	AAATGAAGTAGAAGAGGAGG+AGG	-	chr8_2:33585952-33585971	Msa1244650:three_prime_UTR	40.0%
	AATGAAGTAGAAGAGGAGGA+GGG	-	chr8_2:33585953-33585972	Msa1244650:three_prime_UTR	40.0%
	GAAACTCACACACTTTATGG+CGG	+	chr8_2:33586117-33586136	None:intergenic	40.0%
	AGTGTGTGAGTTTCATTCCA+AGG	-	chr8_2:33586123-33586142	Msa1244650:CDS	40.0%
!!	GGTTTTGTCAACAATGTAGC+AGG	-	chr8_2:33586179-33586198	Msa1244650:CDS	40.0%
	ATCAGATCAGTCACTTCAAC+TGG	-	chr8_2:33586449-33586468	Msa1244650:intron	40.0%
	ATAACTTAATCCCGGCGAAT+TGG	-	chr8_2:33586489-33586508	Msa1244650:intron	40.0%
	CCTATTCAACCGATTGTAAC+GGG	+	chr8_2:33586521-33586540	None:intergenic	40.0%
	CATCAAACTCTACCAAGTCA+TGG	+	chr8_2:33586760-33586779	None:intergenic	40.0%
	CAAAGGACTACATCAACCTA+AGG	-	chr8_2:33586874-33586893	Msa1244650:CDS	40.0%
	AACCTAAGGAAACTCAATGC+AGG	-	chr8_2:33586888-33586907	Msa1244650:CDS	40.0%
	ATCCTGCATTGAGTTTCCTT+AGG	+	chr8_2:33586893-33586912	None:intergenic	40.0%
	AGGATTCAGATGAACTTACC+AGG	-	chr8_2:33586908-33586927	Msa1244650:CDS	40.0%
!!	TGTCACCTTCTGGTTTCTTA+CGG	-	chr8_2:33587016-33587035	Msa1244650:CDS	40.0%
	GTGAACTGACAATGGCTATA+AGG	+	chr8_2:33585836-33585855	None:intergenic	40.0%
	GTTCACCTCTCACAAAACAA+TGG	-	chr8_2:33585850-33585869	Msa1244650:three_prime_UTR	40.0%
!!	AGCTTCCATTGTTTTGTGAG+AGG	+	chr8_2:33585858-33585877	None:intergenic	40.0%
	CTCACAAAACAATGGAAGCT+AGG	-	chr8_2:33585858-33585877	Msa1244650:three_prime_UTR	40.0%
	GAGGAAGAGAACATTGATGA+TGG	-	chr8_2:33585889-33585908	Msa1244650:three_prime_UTR	40.0%
!	CAACATGGTGGAAGAAGAAA+TGG	-	chr8_2:33585928-33585947	Msa1244650:three_prime_UTR	40.0%
	GGAAAATGAAGTAGAAGAGG+AGG	-	chr8_2:33585949-33585968	Msa1244650:three_prime_UTR	40.0%
	AAATGAAGTAGAAGAGGAGG+AGG	-	chr8_2:33585952-33585971	Msa1244650:three_prime_UTR	40.0%
	AATGAAGTAGAAGAGGAGGA+GGG	-	chr8_2:33585953-33585972	Msa1244650:three_prime_UTR	40.0%
	GAAACTCACACACTTTATGG+CGG	+	chr8_2:33586117-33586136	None:intergenic	40.0%
	AGTGTGTGAGTTTCATTCCA+AGG	-	chr8_2:33586123-33586142	Msa1244650:CDS	40.0%
!!	GGTTTTGTCAACAATGTAGC+AGG	-	chr8_2:33586179-33586198	Msa1244650:CDS	40.0%
	ATCAGATCAGTCACTTCAAC+TGG	-	chr8_2:33586449-33586468	Msa1244650:intron	40.0%
	ATAACTTAATCCCGGCGAAT+TGG	-	chr8_2:33586489-33586508	Msa1244650:intron	40.0%
	CCTATTCAACCGATTGTAAC+GGG	+	chr8_2:33586521-33586540	None:intergenic	40.0%
	CATCAAACTCTACCAAGTCA+TGG	+	chr8_2:33586760-33586779	None:intergenic	40.0%
	CAAAGGACTACATCAACCTA+AGG	-	chr8_2:33586874-33586893	Msa1244650:CDS	40.0%
	AACCTAAGGAAACTCAATGC+AGG	-	chr8_2:33586888-33586907	Msa1244650:CDS	40.0%
	ATCCTGCATTGAGTTTCCTT+AGG	+	chr8_2:33586893-33586912	None:intergenic	40.0%
	AGGATTCAGATGAACTTACC+AGG	-	chr8_2:33586908-33586927	Msa1244650:CDS	40.0%
!!	TGTCACCTTCTGGTTTCTTA+CGG	-	chr8_2:33587016-33587035	Msa1244650:CDS	40.0%
	ACTGACAATGGCTATAAGGG+AGG	+	chr8_2:33585832-33585851	None:intergenic	45.0%
	TGTGAGAGGTGAACTGACAA+TGG	+	chr8_2:33585844-33585863	None:intergenic	45.0%
!!	TGGAAGCTAGGAGTATTGAG+AGG	-	chr8_2:33585870-33585889	Msa1244650:three_prime_UTR	45.0%
	GATGAGAAGGAACAACATGG+TGG	-	chr8_2:33585916-33585935	Msa1244650:three_prime_UTR	45.0%
	GAAGAGAAGAAGAAAGGGGT+CGG	-	chr8_2:33585995-33586014	Msa1244650:three_prime_UTR	45.0%
	TGGCCAATTCCCGTTACAAT+CGG	-	chr8_2:33586509-33586528	Msa1244650:intron	45.0%
!	CAACCGATTGTAACGGGAAT+TGG	+	chr8_2:33586515-33586534	None:intergenic	45.0%
	CCCGTTACAATCGGTTGAAT+AGG	-	chr8_2:33586518-33586537	Msa1244650:intron	45.0%
	ATGAACTTACCAGGCAGTTC+TGG	-	chr8_2:33586917-33586936	Msa1244650:CDS	45.0%
	GACTTATAACCAGAACTGCC+TGG	+	chr8_2:33586929-33586948	None:intergenic	45.0%
	TTGGACCGTAAGAAACCAGA+AGG	+	chr8_2:33587024-33587043	None:intergenic	45.0%
!!	TTCTGGTTTCTTACGGTCCA+AGG	-	chr8_2:33587023-33587042	Msa1244650:CDS	45.0%
	ACTGACAATGGCTATAAGGG+AGG	+	chr8_2:33585832-33585851	None:intergenic	45.0%
	TGTGAGAGGTGAACTGACAA+TGG	+	chr8_2:33585844-33585863	None:intergenic	45.0%
!!	TGGAAGCTAGGAGTATTGAG+AGG	-	chr8_2:33585870-33585889	Msa1244650:three_prime_UTR	45.0%
	GATGAGAAGGAACAACATGG+TGG	-	chr8_2:33585916-33585935	Msa1244650:three_prime_UTR	45.0%
	GAAGAGAAGAAGAAAGGGGT+CGG	-	chr8_2:33585995-33586014	Msa1244650:three_prime_UTR	45.0%
	TGGCCAATTCCCGTTACAAT+CGG	-	chr8_2:33586509-33586528	Msa1244650:intron	45.0%
!	CAACCGATTGTAACGGGAAT+TGG	+	chr8_2:33586515-33586534	None:intergenic	45.0%
	CCCGTTACAATCGGTTGAAT+AGG	-	chr8_2:33586518-33586537	Msa1244650:intron	45.0%
	ATGAACTTACCAGGCAGTTC+TGG	-	chr8_2:33586917-33586936	Msa1244650:CDS	45.0%
	GACTTATAACCAGAACTGCC+TGG	+	chr8_2:33586929-33586948	None:intergenic	45.0%
	TTGGACCGTAAGAAACCAGA+AGG	+	chr8_2:33587024-33587043	None:intergenic	45.0%
!!	TTCTGGTTTCTTACGGTCCA+AGG	-	chr8_2:33587023-33587042	Msa1244650:CDS	45.0%
!!	GAGGTGTGGAGCTGATTTGA+CGG	-	chr8_2:33586072-33586091	Msa1244650:CDS	50.0%
!	TCATTCCAAGGCACCTGTTG+TGG	-	chr8_2:33586135-33586154	Msa1244650:CDS	50.0%
	AACGACGAGTTATGCTGTGC+AGG	-	chr8_2:33586732-33586751	Msa1244650:intron	50.0%
	AGTCTAAAAGAAGCTGCCGC+AGG	-	chr8_2:33586780-33586799	Msa1244650:intron	50.0%
!	GGTTGGCTGGACACAATGAA+CGG	-	chr8_2:33586804-33586823	Msa1244650:intron	50.0%
!!!	CAGTTTCCGGGTTGGTTTTC+CGG	+	chr8_2:33586832-33586851	None:intergenic	50.0%
	AACCCGGAAACTGCCAATGA+GGG	-	chr8_2:33586839-33586858	Msa1244650:CDS	50.0%
	TTCCCTCATTGGCAGTTTCC+GGG	+	chr8_2:33586844-33586863	None:intergenic	50.0%
	CTTCCCTCATTGGCAGTTTC+CGG	+	chr8_2:33586845-33586864	None:intergenic	50.0%
	CTCTCTCTTCTGTCACCTTC+TGG	-	chr8_2:33587006-33587025	Msa1244650:CDS	50.0%
!!	GAGGTGTGGAGCTGATTTGA+CGG	-	chr8_2:33586072-33586091	Msa1244650:CDS	50.0%
!	TCATTCCAAGGCACCTGTTG+TGG	-	chr8_2:33586135-33586154	Msa1244650:CDS	50.0%
	AACGACGAGTTATGCTGTGC+AGG	-	chr8_2:33586732-33586751	Msa1244650:intron	50.0%
	AGTCTAAAAGAAGCTGCCGC+AGG	-	chr8_2:33586780-33586799	Msa1244650:intron	50.0%
!	GGTTGGCTGGACACAATGAA+CGG	-	chr8_2:33586804-33586823	Msa1244650:intron	50.0%
!!!	CAGTTTCCGGGTTGGTTTTC+CGG	+	chr8_2:33586832-33586851	None:intergenic	50.0%
	AACCCGGAAACTGCCAATGA+GGG	-	chr8_2:33586839-33586858	Msa1244650:CDS	50.0%
	TTCCCTCATTGGCAGTTTCC+GGG	+	chr8_2:33586844-33586863	None:intergenic	50.0%
	CTTCCCTCATTGGCAGTTTC+CGG	+	chr8_2:33586845-33586864	None:intergenic	50.0%
	CTCTCTCTTCTGTCACCTTC+TGG	-	chr8_2:33587006-33587025	Msa1244650:CDS	50.0%
	GAGAAGAAGAAAGGGGTCGG+TGG	-	chr8_2:33585998-33586017	Msa1244650:three_prime_UTR	55.0%
	AAGAAGAAAGGGGTCGGTGG+CGG	-	chr8_2:33586001-33586020	Msa1244650:three_prime_UTR	55.0%
	CTCTCTGCTTGGCAACAAGG+TGG	+	chr8_2:33586055-33586074	None:intergenic	55.0%
	CACCTTGTTGCCAAGCAGAG+AGG	-	chr8_2:33586053-33586072	Msa1244650:exon	55.0%
	CACCTCTCTGCTTGGCAACA+AGG	+	chr8_2:33586058-33586077	None:intergenic	55.0%
	TGTTGCCAAGCAGAGAGGTG+TGG	-	chr8_2:33586058-33586077	Msa1244650:exon	55.0%
	CACACACTTTATGGCGGCGA+TGG	+	chr8_2:33586111-33586130	None:intergenic	55.0%
!	TTCCAAGGCACCTGTTGTGG+TGG	-	chr8_2:33586138-33586157	Msa1244650:CDS	55.0%
	AACCACCACAACAGGTGCCT+TGG	+	chr8_2:33586143-33586162	None:intergenic	55.0%
	CACCTGTTGTGGTGGTTGCA+GGG	-	chr8_2:33586146-33586165	Msa1244650:CDS	55.0%
	ATCCCTGCAACCACCACAAC+AGG	+	chr8_2:33586151-33586170	None:intergenic	55.0%
	TTGTGGTGGTTGCAGGGATG+AGG	-	chr8_2:33586152-33586171	Msa1244650:CDS	55.0%
	ACGGGAATTGGCCAATTCGC+CGG	+	chr8_2:33586503-33586522	None:intergenic	55.0%
	GCTGTGCAGGTTCCATGACT+TGG	-	chr8_2:33586745-33586764	Msa1244650:intron	55.0%
	CTAAAAGAAGCTGCCGCAGG+CGG	-	chr8_2:33586783-33586802	Msa1244650:intron	55.0%
	CTCATTGGCAGTTTCCGGGT+TGG	+	chr8_2:33586840-33586859	None:intergenic	55.0%
	CAACCCGGAAACTGCCAATG+AGG	-	chr8_2:33586838-33586857	Msa1244650:CDS	55.0%
	GCTGTGACTGCTTCCCTCAT+TGG	+	chr8_2:33586855-33586874	None:intergenic	55.0%
	GAGGGAAGCAGTCACAGCAA+AGG	-	chr8_2:33586857-33586876	Msa1244650:CDS	55.0%
	GGTGACAGAAGAGAGAGAGC+TGG	+	chr8_2:33587003-33587022	None:intergenic	55.0%
	GAGAAGAAGAAAGGGGTCGG+TGG	-	chr8_2:33585998-33586017	Msa1244650:three_prime_UTR	55.0%
	AAGAAGAAAGGGGTCGGTGG+CGG	-	chr8_2:33586001-33586020	Msa1244650:three_prime_UTR	55.0%
	CTCTCTGCTTGGCAACAAGG+TGG	+	chr8_2:33586055-33586074	None:intergenic	55.0%
	CACCTTGTTGCCAAGCAGAG+AGG	-	chr8_2:33586053-33586072	Msa1244650:exon	55.0%
	CACCTCTCTGCTTGGCAACA+AGG	+	chr8_2:33586058-33586077	None:intergenic	55.0%
	TGTTGCCAAGCAGAGAGGTG+TGG	-	chr8_2:33586058-33586077	Msa1244650:exon	55.0%
	CACACACTTTATGGCGGCGA+TGG	+	chr8_2:33586111-33586130	None:intergenic	55.0%
!	TTCCAAGGCACCTGTTGTGG+TGG	-	chr8_2:33586138-33586157	Msa1244650:CDS	55.0%
	AACCACCACAACAGGTGCCT+TGG	+	chr8_2:33586143-33586162	None:intergenic	55.0%
	CACCTGTTGTGGTGGTTGCA+GGG	-	chr8_2:33586146-33586165	Msa1244650:CDS	55.0%
	ATCCCTGCAACCACCACAAC+AGG	+	chr8_2:33586151-33586170	None:intergenic	55.0%
	TTGTGGTGGTTGCAGGGATG+AGG	-	chr8_2:33586152-33586171	Msa1244650:CDS	55.0%
	ACGGGAATTGGCCAATTCGC+CGG	+	chr8_2:33586503-33586522	None:intergenic	55.0%
	GCTGTGCAGGTTCCATGACT+TGG	-	chr8_2:33586745-33586764	Msa1244650:intron	55.0%
	CTAAAAGAAGCTGCCGCAGG+CGG	-	chr8_2:33586783-33586802	Msa1244650:intron	55.0%
	CTCATTGGCAGTTTCCGGGT+TGG	+	chr8_2:33586840-33586859	None:intergenic	55.0%
	CAACCCGGAAACTGCCAATG+AGG	-	chr8_2:33586838-33586857	Msa1244650:CDS	55.0%
	GCTGTGACTGCTTCCCTCAT+TGG	+	chr8_2:33586855-33586874	None:intergenic	55.0%
	GAGGGAAGCAGTCACAGCAA+AGG	-	chr8_2:33586857-33586876	Msa1244650:CDS	55.0%
	GGTGACAGAAGAGAGAGAGC+TGG	+	chr8_2:33587003-33587022	None:intergenic	55.0%
	AGAAGAAAGGGGTCGGTGGC+GGG	-	chr8_2:33586002-33586021	Msa1244650:three_prime_UTR	60.0%
	CAGCTCCACACCTCTCTGCT+TGG	+	chr8_2:33586066-33586085	None:intergenic	60.0%
	GCACCTGTTGTGGTGGTTGC+AGG	-	chr8_2:33586145-33586164	Msa1244650:CDS	60.0%
	TGGTTGCAGGGATGAGGCAG+AGG	-	chr8_2:33586158-33586177	Msa1244650:CDS	60.0%
	CGGGAATTGGCCAATTCGCC+GGG	+	chr8_2:33586502-33586521	None:intergenic	60.0%
	CTGGACACAATGAACGGCGC+CGG	-	chr8_2:33586810-33586829	Msa1244650:intron	60.0%
	AGAAGAAAGGGGTCGGTGGC+GGG	-	chr8_2:33586002-33586021	Msa1244650:three_prime_UTR	60.0%
	CAGCTCCACACCTCTCTGCT+TGG	+	chr8_2:33586066-33586085	None:intergenic	60.0%
	GCACCTGTTGTGGTGGTTGC+AGG	-	chr8_2:33586145-33586164	Msa1244650:CDS	60.0%
	TGGTTGCAGGGATGAGGCAG+AGG	-	chr8_2:33586158-33586177	Msa1244650:CDS	60.0%
	CGGGAATTGGCCAATTCGCC+GGG	+	chr8_2:33586502-33586521	None:intergenic	60.0%
	CTGGACACAATGAACGGCGC+CGG	-	chr8_2:33586810-33586829	Msa1244650:intron	60.0%
!	GGGAGAAGAGGAGGTGGTGG+CGG	-	chr8_2:33586022-33586041	Msa1244650:three_prime_UTR	65.0%
	AAGAAGCTGCCGCAGGCGGT+TGG	-	chr8_2:33586787-33586806	Msa1244650:intron	65.0%
	TGTGTCCAGCCAACCGCCTG+CGG	+	chr8_2:33586799-33586818	None:intergenic	65.0%
!	ACGGCGCCGGAAAACCAACC+CGG	-	chr8_2:33586823-33586842	Msa1244650:intron	65.0%
!	GGGAGAAGAGGAGGTGGTGG+CGG	-	chr8_2:33586022-33586041	Msa1244650:three_prime_UTR	65.0%
	AAGAAGCTGCCGCAGGCGGT+TGG	-	chr8_2:33586787-33586806	Msa1244650:intron	65.0%
	TGTGTCCAGCCAACCGCCTG+CGG	+	chr8_2:33586799-33586818	None:intergenic	65.0%
!	ACGGCGCCGGAAAACCAACC+CGG	-	chr8_2:33586823-33586842	Msa1244650:intron	65.0%
!!	GTCGGTGGCGGGAGAAGAGG+AGG	-	chr8_2:33586013-33586032	Msa1244650:three_prime_UTR	70.0%
!!	GGTGGCGGGAGAAGAGGAGG+TGG	-	chr8_2:33586016-33586035	Msa1244650:three_prime_UTR	70.0%
	GGCGGGAGAAGAGGAGGTGG+TGG	-	chr8_2:33586019-33586038	Msa1244650:three_prime_UTR	70.0%
!!	GTCGGTGGCGGGAGAAGAGG+AGG	-	chr8_2:33586013-33586032	Msa1244650:three_prime_UTR	70.0%
!!	GGTGGCGGGAGAAGAGGAGG+TGG	-	chr8_2:33586016-33586035	Msa1244650:three_prime_UTR	70.0%
	GGCGGGAGAAGAGGAGGTGG+TGG	-	chr8_2:33586019-33586038	Msa1244650:three_prime_UTR	70.0%
!!	GGGGTCGGTGGCGGGAGAAG+AGG	-	chr8_2:33586010-33586029	Msa1244650:three_prime_UTR	75.0%
	AGCTGCCGCAGGCGGTTGGC+TGG	-	chr8_2:33586791-33586810	Msa1244650:intron	75.0%
!!	GGGGTCGGTGGCGGGAGAAG+AGG	-	chr8_2:33586010-33586029	Msa1244650:three_prime_UTR	75.0%
	AGCTGCCGCAGGCGGTTGGC+TGG	-	chr8_2:33586791-33586810	Msa1244650:intron	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	33585784	33587240	33585784	ID=Msa1244650;Name=Msa1244650
chr8_2	mRNA	33585784	33587240	33585784	ID=Msa1244650-mRNA-1;Parent=Msa1244650;Name=Msa1244650-mRNA-1;_AED=0.04;_eAED=0.04;_QI=85\|1\|1\|1\|1\|1\|2\|276\|180
chr8_2	exon	33585784	33586270	33585784	ID=Msa1244650-mRNA-1:exon:10798;Parent=Msa1244650-mRNA-1
chr8_2	exon	33586824	33587240	33586824	ID=Msa1244650-mRNA-1:exon:10797;Parent=Msa1244650-mRNA-1
chr8_2	five_prime_UTR	33587156	33587240	33587156	ID=Msa1244650-mRNA-1:five_prime_utr;Parent=Msa1244650-mRNA-1
chr8_2	CDS	33586824	33587155	33586824	ID=Msa1244650-mRNA-1:cds;Parent=Msa1244650-mRNA-1
chr8_2	CDS	33586060	33586270	33586060	ID=Msa1244650-mRNA-1:cds;Parent=Msa1244650-mRNA-1
chr8_2	three_prime_UTR	33585784	33586059	33585784	ID=Msa1244650-mRNA-1:three_prime_utr;Parent=Msa1244650-mRNA-1

Gene Sequence

>Msa1244650

ATGGAAGCTAGGAGTATTGAGAGGAAGAGAACATTGATGATGGAAAAGATGAGAAGGAACAACATGGTGGAAGAAGAAATGGAAAATGAAGTAGAAGAGGAGGAGGGTGATGTTAGTCTAACTGAAGAAGAGAAGAAGAAAGGGGTCGGTGGCGGGAGAAGAGGAGGTGGTGGCGGTGTTTCTCCACCTTGTTGCCAAGCAGAGAGGTGTGGAGCTGATTTGACGGATGCAAAGAAATACCATCGCCGCCATAAAGTGTGTGAGTTTCATTCCAAGGCACCTGTTGTGGTGGTTGCAGGGATGAGGCAGAGGTTTTGTCAACAATGTAGCAGGTTCCATGACTTGGTAGAGTTTGATGAGTCTAAAAGAAGCTGCCGCAGGCGGTTGGCTGGACACAATGAACGGCGCCGGAAAACCAACCCGGAAACTGCCAATGAGGGAAGCAGTCACAGCAAAGGACTACATCAACCTAAGGAAACTCAATGCAGGATTCAGATGAACTTACCAGGCAGTTCTGGTTATAAGTCCTTCAACATTAGATGA

Protein sequence

>Msa1244650

MEARSIERKRTLMMEKMRRNNMVEEEMENEVEEEEGDVSLTEEEKKKGVGGGRRGGGGGVSPPCCQAERCGADLTDAKKYHRRHKVCEFHSKAPVVVVAGMRQRFCQQCSRFHDLVEFDESKRSCRRRLAGHNERRRKTNPETANEGSSHSKGLHQPKETQCRIQMNLPGSSGYKSFNIR*