AlfalfaGEDB | Msa1077950

M. sativa cultivar zhongmu-4 / Msa1077950

KEGG: K13265
GO: -
PFAM: PF01370.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1077950	XP_003623719.1	97.203	286	8	0	14	299	41	326	0.0	575

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1077950	sp\|Q40316\|VESTR_MEDSA	68.881	286	89	0	14	299	41	326	1.15e-147	420

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1077950	A2Q3Y3	97.203	286	8	0	14	299	41	326	0.0	575

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0033340	Msa1077950	0.847351	1.221695e-59	-8.615850e-47
Msa0054260	Msa1077950	0.817762	2.786706e-52	-8.615850e-47
Msa0054310	Msa1077950	0.804953	1.686301e-49	-8.615850e-47
Msa0055690	Msa1077950	0.814720	1.334586e-51	-8.615850e-47
Msa0055730	Msa1077950	0.833108	6.445817e-56	-8.615850e-47
Msa0085360	Msa1077950	-0.805993	1.020768e-49	-8.615850e-47
Msa0100090	Msa1077950	0.821343	4.247294e-53	-8.615850e-47
Msa0165510	Msa1077950	0.800378	1.482422e-48	-8.615850e-47
Msa0173990	Msa1077950	0.832850	7.471342e-56	-8.615850e-47
Msa0197660	Msa1077950	0.838766	2.366622e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1077950	MtrunA17_Chr7g0245741	97.203	286	8	0	14	299	41	326	0.0	575
Msa1077950	MtrunA17_Chr7g0245711	90.559	286	27	0	14	299	3	288	0.0	541
Msa1077950	MtrunA17_Chr7g0245731	90.559	286	27	0	14	299	91	376	0.0	541
Msa1077950	MtrunA17_Chr7g0245641	75.084	297	72	1	5	299	70	366	3.66e-170	476
Msa1077950	MtrunA17_Chr7g0245671	74.658	292	71	2	11	299	38	329	7.11e-163	456
Msa1077950	MtrunA17_Chr7g0245691	71.812	298	81	1	5	299	33	330	4.64e-162	454
Msa1077950	MtrunA17_Chr7g0245631	69.231	286	88	0	14	299	41	326	4.87e-149	421
Msa1077950	MtrunA17_Chr4g0010991	46.939	294	154	2	8	299	36	329	3.78e-87	263
Msa1077950	MtrunA17_Chr1g0155411	44.912	285	153	3	17	299	43	325	2.47e-82	251
Msa1077950	MtrunA17_Chr1g0155431	45.139	288	154	3	14	299	40	325	4.22e-80	246

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1077950	AT2G45400.3	48.621	290	142	4	16	299	19	307	1.53e-93	280
Msa1077950	AT2G45400.1	46.865	303	154	4	3	299	63	364	5.17e-93	280
Msa1077950	AT2G45400.2	50.000	244	116	3	16	253	76	319	1.63e-80	247
Msa1077950	AT5G42800.1	43.945	289	150	3	17	299	43	325	6.67e-77	239
Msa1077950	AT4G35420.1	38.194	288	166	7	16	299	42	321	1.49e-62	201
Msa1077950	AT1G61720.1	37.919	298	168	7	14	299	45	337	2.54e-57	188
Msa1077950	AT2G45400.4	49.198	187	90	2	8	189	68	254	1.18e-56	183
Msa1077950	AT1G68540.1	34.114	299	185	7	6	299	30	321	5.37e-53	176
Msa1077950	AT1G25460.1	33.904	292	184	5	12	299	33	319	5.43e-52	173
Msa1077950	AT2G02400.1	35.616	292	165	8	18	299	40	318	1.39e-49	167

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Find 49 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGGTCCAAGAACAAAAGT+TGG	0.557011	7_2:+76753766	None:intergenic
AATCAATTGGAGTTGCAGTG+TGG	0.684696	7_2:+76754088	None:intergenic
ACACAGCCTTGAGGAAATGT+TGG	0.550570	7_2:-76751612	Msa1077950:CDS
ACAGAGCGCAAGAAAGATGT+TGG	0.569599	7_2:-76754219	Msa1077950:intron
ACCAAATAACAAAGACAGAG+AGG	0.665667	7_2:+76753711	None:intergenic
ACCTCTCTGTCTTTGTTATT+TGG	0.159764	7_2:-76753712	Msa1077950:intron
ACTAGAACTCACTCTAGTGT+TGG	0.602184	7_2:+76751798	None:intergenic
AGAACAATTGATGGAGCATT+AGG	0.319772	7_2:-76754030	Msa1077950:CDS
AGAGAGGTATAAATTGAGCC+AGG	0.580168	7_2:+76753727	None:intergenic
AGATAAAGATGATGATGTAA+TGG	0.533409	7_2:-76753929	Msa1077950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTAGAATAAATATCTGTCGA+TGG	+	chr7_2:76751484-76751503	None:intergenic	30.0%
AACTCCAGGGAAAAAAATTA+TGG	-	chr7_2:76751602-76751621	Msa1077950:CDS	30.0%
ACTAGTTAGAGATGTCATAA+TGG	+	chr7_2:76751643-76751662	None:intergenic	30.0%
GTGACAAAAAGAACAATTGA+TGG	-	chr7_2:76752010-76752029	Msa1077950:intron	30.0%
ATTGAATATGACCAAGCAAA+TGG	+	chr7_2:76752190-76752209	None:intergenic	30.0%
CATTTCCAAAGCTAATTTGA+TGG	+	chr7_2:76752729-76752748	None:intergenic	30.0%
TGTTAGTGTATCTAATTGTG+TGG	-	chr7_2:76752779-76752798	Msa1077950:intron	30.0%
TGTATCTAATTGTGTGGATT+AGG	-	chr7_2:76752785-76752804	Msa1077950:intron	30.0%
TGTTGAGTATGATGACTTAT+AGG	-	chr7_2:76752839-76752858	Msa1077950:intron	30.0%
CAACTTGTATTTAAATACCG+CGG	+	chr7_2:76752984-76753003	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	76751472	76754599	76751472	ID=Msa1077950;Name=Msa1077950
chr7_2	mRNA	76751472	76754599	76751472	ID=Msa1077950-mRNA-1;Parent=Msa1077950;Name=Msa1077950-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0.66\|0.25\|0.75\|1\|1\|4\|99\|299
chr7_2	exon	76751472	76751712	76751472	ID=Msa1077950-mRNA-1:exon:18640;Parent=Msa1077950-mRNA-1
chr7_2	exon	76751810	76752002	76751810	ID=Msa1077950-mRNA-1:exon:18639;Parent=Msa1077950-mRNA-1
chr7_2	exon	76753713	76754237	76753713	ID=Msa1077950-mRNA-1:exon:18638;Parent=Msa1077950-mRNA-1
chr7_2	exon	76754560	76754599	76754560	ID=Msa1077950-mRNA-1:exon:18637;Parent=Msa1077950-mRNA-1
chr7_2	CDS	76754560	76754599	76754560	ID=Msa1077950-mRNA-1:cds;Parent=Msa1077950-mRNA-1
chr7_2	CDS	76753713	76754237	76753713	ID=Msa1077950-mRNA-1:cds;Parent=Msa1077950-mRNA-1
chr7_2	CDS	76751810	76752002	76751810	ID=Msa1077950-mRNA-1:cds;Parent=Msa1077950-mRNA-1
chr7_2	CDS	76751571	76751712	76751571	ID=Msa1077950-mRNA-1:cds;Parent=Msa1077950-mRNA-1
chr7_2	three_prime_UTR	76751472	76751570	76751472	ID=Msa1077950-mRNA-1:three_prime_utr;Parent=Msa1077950-mRNA-1

Gene Sequence

>Msa1077950

ATGAGATCTCCATCGACAGATATTTATTCTAGTAGAAAAGAGCGCAAGAAAGATGTTGGCTTCCTCACAAAACTTCCAGGTGCATCCCAAAAGCTAAAATTCTTCAATGCTGATCTAAGCATCCCAGAAAGTTTCAATGCAGCAATTGAAGGATGCAAAGGAATATTCCACACTGCAACTCCAATTGATTTGGAAATGAATGAATCAGAAGAAACAGTGACAAAAAGAACAATTGATGGAGCATTAGGAATTTTAAAAGCATGCAAAAATTCAAAGACAGTGAAGAGAGTTATTTACACTTCAAGTGCTTCAGCAGTGTATTGGCAAGATAAAGATGATGATGTAATGGATGAGAGTTATTGGAGTGATGAAAATCTTCTTAGAGATTTGAAACCATTTGCTTGGTCATATTCAATTTCTAAAACAATGGCAGAGAAGGCTGTTCTAGAGTTTGGAGAACAACATGGTTTAGATGTTGTGACTATAATACCAACTTTTGTTCTTGGACCCTTCATTTGTCCTAAGCGTCCTGGCTCAATTTATACCTCTCTGTCTTTGTTATTTGGGGACAAGAATCCATTTGGCTTCTCTCGTCTCCATATGGTGCATGTTGATGATATTGCAAGAGCACATATATTTCTGCTTGAGCATCCTAATCCAAAAGGGAGATATAATTGCTCACCATTCATTGCAAATATTGAAGAAATAGCTCAACTTATTTCTGCCAAATACCCTGAATTTCATATACCAACACTAGAGGAGCTGAAGGAAATTAAAGGTGACAAGCTTCCACATTTAACCTCAAAAAAACTCATGGATGCTGGATTCGAGTTCAAACACAGCCTTGAGGAAATGTTGGACGATACGATTCAGTGTTGCAAGGAAAAGGGTTATCTTTGA

Protein sequence

>Msa1077950

MRSPSTDIYSSRKERKKDVGFLTKLPGASQKLKFFNADLSIPESFNAAIEGCKGIFHTATPIDLEMNESEETVTKRTIDGALGILKACKNSKTVKRVIYTSSASAVYWQDKDDDVMDESYWSDENLLRDLKPFAWSYSISKTMAEKAVLEFGEQHGLDVVTIIPTFVLGPFICPKRPGSIYTSLSLLFGDKNPFGFSRLHMVHVDDIARAHIFLLEHPNPKGRYNCSPFIANIEEIAQLISAKYPEFHIPTLEELKEIKGDKLPHLTSKKLMDAGFEFKHSLEEMLDDTIQCCKEKGYL*