AlfalfaGEDB | Msa1221700

M. sativa cultivar zhongmu-4 / Msa1221700

KEGG: K00991
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006081 GO:0006082 GO:0006090 GO:0006629 GO:0006644 GO:0006720 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009240 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0019288 GO:0019637 GO:0019682 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046490 GO:0050518 GO:0070567 GO:0071704 GO:0090407 GO:1901135 GO:1901576
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0048000	Msa1221700	0.801590	8.379799e-49	-8.615850e-47
Msa0101810	Msa1221700	-0.811267	7.623776e-51	-8.615850e-47
Msa0102750	Msa1221700	0.800380	1.481240e-48	-8.615850e-47
Msa0610890	Msa1221700	0.806412	8.330987e-50	-8.615850e-47
Msa0647830	Msa1221700	0.805137	1.543178e-49	-8.615850e-47
Msa0658570	Msa1221700	0.819068	1.410142e-52	-8.615850e-47
Msa0690440	Msa1221700	0.806568	7.723394e-50	-8.615850e-47
Msa0693380	Msa1221700	0.809342	1.984146e-50	-8.615850e-47
Msa0703490	Msa1221700	0.809188	2.141007e-50	-8.615850e-47
Msa0715720	Msa1221700	0.802513	5.412783e-49	-8.615850e-47
Msa0802470	Msa1221700	-0.800124	1.669403e-48	-8.615850e-47
Msa0828540	Msa1221700	0.839774	1.295431e-57	-8.615850e-47
Msa0166870	Msa1221700	0.805494	1.299040e-49	-8.615850e-47
Msa0217400	Msa1221700	0.813707	2.233482e-51	-8.615850e-47
Msa0223410	Msa1221700	-0.808796	2.596488e-50	-8.615850e-47
Msa0376590	Msa1221700	0.800426	1.449663e-48	-8.615850e-47
Msa0436370	Msa1221700	0.817421	3.325987e-52	-8.615850e-47
Msa0448550	Msa1221700	-0.822557	2.222429e-53	-8.615850e-47
Msa0473240	Msa1221700	0.810433	1.155680e-50	-8.615850e-47
Msa0990960	Msa1221700	0.802858	4.594602e-49	-8.615850e-47
Msa1001200	Msa1221700	0.803901	2.794160e-49	-8.615850e-47
Msa1037700	Msa1221700	-0.802255	6.118422e-49	-8.615850e-47
Msa1045200	Msa1221700	0.806558	7.761242e-50	-8.615850e-47
Msa1059570	Msa1221700	0.803050	4.194643e-49	-8.615850e-47
Msa1068200	Msa1221700	-0.822395	2.424039e-53	-8.615850e-47
Msa1075080	Msa1221700	-0.807316	5.364253e-50	-8.615850e-47
Msa1075220	Msa1221700	-0.838315	3.093295e-57	-8.615850e-47
Msa1082500	Msa1221700	0.825585	4.325494e-54	-8.615850e-47
Msa1084260	Msa1221700	0.814218	1.723011e-51	-8.615850e-47
Msa0257250	Msa1221700	0.804532	2.064555e-49	-8.615850e-47
Msa0258760	Msa1221700	0.813537	2.434180e-51	-8.615850e-47
Msa0260810	Msa1221700	0.804187	2.436645e-49	-8.615850e-47
Msa0266000	Msa1221700	-0.806683	7.303471e-50	-8.615850e-47
Msa0296560	Msa1221700	0.818659	1.746303e-52	-8.615850e-47
Msa0323080	Msa1221700	0.806299	8.802031e-50	-8.615850e-47
Msa0335040	Msa1221700	0.807233	5.586300e-50	-8.615850e-47
Msa0354800	Msa1221700	-0.815017	1.146536e-51	-8.615850e-47
Msa0356890	Msa1221700	-0.805602	1.233411e-49	-8.615850e-47
Msa1116130	Msa1221700	-0.820063	8.361520e-53	-8.615850e-47
Msa1116440	Msa1221700	0.811335	7.369708e-51	-8.615850e-47
Msa1139000	Msa1221700	-0.820871	5.453564e-53	-8.615850e-47
Msa1156340	Msa1221700	-0.801749	7.775808e-49	-8.615850e-47
Msa1162550	Msa1221700	-0.827541	1.477834e-54	-8.615850e-47
Msa1221700	Msa1221910	0.879755	9.619720e-70	-8.615850e-47
Msa1221700	Msa1229600	0.817936	2.546287e-52	-8.615850e-47
Msa1221700	Msa1267610	0.801931	7.134904e-49	-8.615850e-47
Msa1221700	Msa1271300	0.816958	4.228657e-52	-8.615850e-47
Msa1221700	Msa1298260	0.800661	1.297912e-48	-8.615850e-47
Msa1221700	Msa1310210	0.805242	1.467434e-49	-8.615850e-47
Msa1221700	Msa1314340	0.800249	1.575032e-48	-8.615850e-47
Msa1221700	Msa1322890	0.803817	2.908757e-49	-8.615850e-47
Msa1221700	Msa1329700	0.805221	1.481864e-49	-8.615850e-47
Msa1221700	Msa1376890	0.820794	5.681203e-53	-8.615850e-47
Msa1221700	Msa1379850	0.817734	2.827559e-52	-8.615850e-47
Msa1221700	Msa1393410	0.821046	4.972948e-53	-8.615850e-47
Msa1221700	Msa1404540	0.810237	1.273413e-50	-8.615850e-47
Msa1221700	Msa1435420	0.819192	1.321470e-52	-8.615850e-47
Msa1221700	Msa1451620	-0.828935	6.817287e-55	-8.615850e-47
Msa1221700	Msa1454860	0.829447	5.122537e-55	-8.615850e-47
Msa0484780	Msa1221700	0.828844	7.171651e-55	-8.615850e-47
Msa0492880	Msa1221700	0.804956	1.684326e-49	-8.615850e-47
Msa0498220	Msa1221700	0.810177	1.312048e-50	-8.615850e-47
Msa0516370	Msa1221700	0.806622	7.521935e-50	-8.615850e-47
Msa0532350	Msa1221700	0.817133	3.862418e-52	-8.615850e-47
Msa0536220	Msa1221700	0.806000	1.017172e-49	-8.615850e-47
Msa0553460	Msa1221700	0.809508	1.827856e-50	-8.615850e-47
Msa0558690	Msa1221700	0.819898	9.118286e-53	-8.615850e-47
Msa0563840	Msa1221700	0.804647	1.953997e-49	-8.615850e-47
Msa0567020	Msa1221700	0.812859	3.429398e-51	-8.615850e-47
Msa0572420	Msa1221700	0.802347	5.858367e-49	-8.615850e-47
Msa0572480	Msa1221700	0.808006	3.828387e-50	-8.615850e-47
Msa0870530	Msa1221700	0.811824	5.769426e-51	-8.615850e-47
Msa0903700	Msa1221700	0.819713	1.005089e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221700	MtrunA17_Chr4g0076791	90.625	32	3	0	2	33	218	249	5.58e-14	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221700	AT2G02500.1	71.875	32	9	0	2	33	215	246	7.84e-11	55.5
Msa1221700	AT2G02500.2	71.875	32	9	0	2	33	215	246	7.84e-11	55.5

Find 11 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAGAGGATTTGTACCTGGC+TGG	0.457541	8_1:+85504402	Msa1221690:intron
ACTAGGGATTTCAGCATGGT+TGG	0.492803	8_1:+85504120	Msa1221690:intron
ATCATTTGCCGTTAGAACAC+TGG	0.515238	8_1:-85504581	Msa1221700:CDS
TAATATAATTCTCAGCAACT+CGG	0.518851	8_1:+85504429	Msa1221690:intron
TGAGAAACTGTGCAGATCAC+AGG	0.531427	8_1:-85504098	None:intergenic
ATCTGCACAGTTTCTCACTA+GGG	0.559582	8_1:+85504104	Msa1221690:intron
AATTATATTAATATCCAGCC+AGG	0.566677	8_1:-85504416	Msa1221700:intron
AATATAATTCTCAGCAACTC+GGG	0.573122	8_1:+85504430	Msa1221690:intron
GATCTGCACAGTTTCTCACT+AGG	0.574102	8_1:+85504103	Msa1221690:intron
ATGGGAGATGCAGACCCCAC+AGG	0.600097	8_1:-85504545	Msa1221700:intron
TCTCACTAGGGATTTCAGCA+TGG	0.604577	8_1:+85504116	Msa1221690:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAATTGAGAAATATTTA+TGG	+	chr8_1:85504408-85504427	Msa1221690:intron	10.0%
!!!	ATGTACATCTCATTTATTTT+TGG	-	chr8_1:85504194-85504213	Msa1221700:intron	20.0%
!	TAATATAATTCTCAGCAACT+CGG	+	chr8_1:85504271-85504290	Msa1221690:intron	25.0%
!	AATTATATTAATATCCAGCC+AGG	-	chr8_1:85504281-85504300	Msa1221700:intron	25.0%
!	AAAGAAGCAAAATGATCAAA+TGG	-	chr8_1:85504318-85504337	Msa1221700:intron	25.0%
!	GTAGATATGAAAGATTAACT+TGG	+	chr8_1:85504533-85504552	Msa1221690:intron	25.0%
	AATATAATTCTCAGCAACTC+GGG	+	chr8_1:85504270-85504289	Msa1221690:intron	30.0%
!	ATCATTTTGCTTCTTTTCAG+AGG	+	chr8_1:85504314-85504333	Msa1221690:intron	30.0%
!	TTTTCTGTCCAGTGTTCTAA+CGG	+	chr8_1:85504127-85504146	Msa1221690:intron	35.0%
!	ATCTTTTCAGTTTGACCTGT+GGG	+	chr8_1:85504170-85504189	Msa1221690:intron	35.0%
	AGCATTGTAAGTAGTTGACT+AGG	+	chr8_1:85504223-85504242	Msa1221690:intron	35.0%
!!	ATAGCATGCAGTGCTTTTTA+CGG	+	chr8_1:85504446-85504465	Msa1221690:intron	35.0%
	AAACCATTTCTTCAGTCACA+TGG	-	chr8_1:85504475-85504494	Msa1221700:intron	35.0%
	TTTCCATGTGACTGAAGAAA+TGG	+	chr8_1:85504481-85504500	Msa1221690:intron	35.0%
	ATCATTTGCCGTTAGAACAC+TGG	-	chr8_1:85504116-85504135	Msa1221700:CDS	40.0%
	ACTGGACAGAAAAACGCTAT+GGG	-	chr8_1:85504134-85504153	Msa1221700:CDS	40.0%
!	TCTTTTCAGTTTGACCTGTG+GGG	+	chr8_1:85504169-85504188	Msa1221690:intron	40.0%
!	CATCTTTTCAGTTTGACCTG+TGG	+	chr8_1:85504171-85504190	Msa1221690:intron	40.0%
!	CTTTTCAGAGGATTTGTACC+TGG	+	chr8_1:85504302-85504321	Msa1221690:intron	40.0%
	ATCTGCACAGTTTCTCACTA+GGG	+	chr8_1:85504596-85504615	Msa1221690:intron	40.0%
	CACTGGACAGAAAAACGCTA+TGG	-	chr8_1:85504133-85504152	Msa1221700:CDS	45.0%
	ACTAGGGATTTCAGCATGGT+TGG	+	chr8_1:85504580-85504599	Msa1221690:intron	45.0%
	TCTCACTAGGGATTTCAGCA+TGG	+	chr8_1:85504584-85504603	Msa1221690:intron	45.0%
	GATCTGCACAGTTTCTCACT+AGG	+	chr8_1:85504597-85504616	Msa1221690:intron	45.0%
	TCAGAGGATTTGTACCTGGC+TGG	+	chr8_1:85504298-85504317	Msa1221690:intron	50.0%
	ATGGGAGATGCAGACCCCAC+AGG	-	chr8_1:85504152-85504171	Msa1221700:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	85504099	85504620	85504099	ID=Msa1221700;Name=Msa1221700
chr8_1	mRNA	85504099	85504620	85504099	ID=Msa1221700-mRNA-1;Parent=Msa1221700;Name=Msa1221700-mRNA-1;_AED=0.39;_eAED=0.43;_QI=0\|0\|0\|1\|1\|1\|3\|0\|60
chr8_1	exon	85504546	85504620	85504546	ID=Msa1221700-mRNA-1:exon:30774;Parent=Msa1221700-mRNA-1
chr8_1	exon	85504417	85504461	85504417	ID=Msa1221700-mRNA-1:exon:30773;Parent=Msa1221700-mRNA-1
chr8_1	exon	85504099	85504158	85504099	ID=Msa1221700-mRNA-1:exon:30772;Parent=Msa1221700-mRNA-1
chr8_1	CDS	85504546	85504620	85504546	ID=Msa1221700-mRNA-1:cds;Parent=Msa1221700-mRNA-1
chr8_1	CDS	85504417	85504461	85504417	ID=Msa1221700-mRNA-1:cds;Parent=Msa1221700-mRNA-1
chr8_1	CDS	85504099	85504158	85504099	ID=Msa1221700-mRNA-1:cds;Parent=Msa1221700-mRNA-1

Gene Sequence	>Msa1221700 CTGCAGGCTAACACTAAATCATTTGCCGTTAGAACACTGGACAGAAAAACGCTATGGGAGATGCAGACCCCACAGATTATCAAACCCGAGTTGCTGAGAATTATATTAATATCCAGCCAGATAACTTGTACTGCATCCAACCATGCTGAAATCCCTAGTGAGAAACTGTGCAGATCACAG
Protein sequence	>Msa1221700 LQANTKSFAVRTLDRKTLWEMQTPQIIKPELLRIILISSQITCTASNHAEIPSEKLCRSQ