AlfalfaGEDB | Msa1375990

M. sativa cultivar zhongmu-4 / Msa1375990

KEGG: -
GO: -
PFAM: PF08991.13

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1375990	KEH32768.1	97.101	69	2	0	1	69	1	69	1.07e-39	135

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1375990	A0A072UUM7	97.101	69	2	0	1	69	1	69	5.12e-40	135

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0057140	Msa1375990	0.802730	4.882694e-49	-8.615850e-47
Msa0064350	Msa1375990	0.827400	1.597541e-54	-8.615850e-47
Msa0137040	Msa1375990	0.804760	1.851016e-49	-8.615850e-47
Msa0173740	Msa1375990	0.804298	2.309806e-49	-8.615850e-47
Msa0173820	Msa1375990	0.802876	4.556798e-49	-8.615850e-47
Msa0178990	Msa1375990	0.802002	6.896061e-49	-8.615850e-47
Msa0183460	Msa1375990	0.821992	3.005309e-53	-8.615850e-47
Msa0191050	Msa1375990	0.807505	4.891422e-50	-8.615850e-47
Msa0194480	Msa1375990	0.818558	1.841288e-52	-8.615850e-47
Msa0199690	Msa1375990	0.828223	1.013146e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1375990	MtrunA17_Chr4g0077101	97.101	69	2	0	1	69	1	69	9.85e-44	135

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1375990	AT1G09794.1	69.841	63	19	0	1	63	1	63	2.34e-27	94.0

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Find 43 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTAAATTCACAATTGC+TGG	0.339243	tig0019777:-66035	None:intergenic
AAACTTAAATTCACAATTGC+TGG	0.339243	tig0019777:-66211	None:intergenic
AAGGTTTGAGAAAATGGGGC+AGG	0.531461	tig0019777:+64639	Msa1375990:exon
AATCTCCCTACCCAGCATTG+GGG	0.629498	tig0019777:-66010	None:intergenic
AATCTCCCTACCCAGCATTG+GGG	0.629498	tig0019777:-66186	None:intergenic
ACACAAATATCAATTGCAAT+TGG	0.443864	tig0019777:-66308	None:intergenic
ACTCACTGTGCTTCGCTCTC+TGG	0.391788	tig0019777:+65741	Msa1375990:CDS
AGAAAGGGACACCCCAATGC+TGG	0.454087	tig0019777:+65999	Msa1375990:three_prime_UTR
AGAAAGGGACACCCCAATGC+TGG	0.454087	tig0019777:+66175	Msa1375990:three_prime_UTR
AGTAATTGGATAAGTAGTTG+AGG	0.616983	tig0019777:+65885	Msa1375990:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGAAGAAGGTTTGAGAAAA+TGG	+	tig0019777:64633-64652	Msa1375990:exon	30.0%
TGAAGAAGGTTTGAGAAAAT+GGG	+	tig0019777:64634-64653	Msa1375990:exon	30.0%
AGTTAGAAAACTTTCTTCTG+CGG	+	tig0019777:65133-65152	Msa1375990:intron	30.0%
AGCAAATATTGGTAAGTTAG+TGG	+	tig0019777:65505-65524	Msa1375990:intron	30.0%
CATTGTTGAAACAAAACTGT+TGG	+	tig0019777:65622-65641	Msa1375990:intron	30.0%
CCAGCAATTCAATAACTTTA+AGG	-	tig0019777:65689-65708	None:intergenic	30.0%
CCTTAAAGTTATTGAATTGC+TGG	+	tig0019777:65686-65705	Msa1375990:CDS	30.0%
CAAAACAAAAATATGCAAGC+CGG	-	tig0019777:65841-65860	None:intergenic	30.0%
CAACTACTTATCCAATTACT+TGG	-	tig0019777:65885-65904	None:intergenic	30.0%
AGTAATTGGATAAGTAGTTG+AGG	+	tig0019777:65885-65904	Msa1375990:exon	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0019777	gene	64567	66361	64567	ID=Msa1375990;Name=Msa1375990
tig0019777	mRNA	64567	66361	64567	ID=Msa1375990-mRNA-1;Parent=Msa1375990;Name=Msa1375990-mRNA-1;_AED=0.09;_eAED=0.09;_QI=85\|1\|1\|1\|1\|1\|3\|472\|69
tig0019777	exon	64567	64737	64567	ID=Msa1375990-mRNA-1:exon:4808;Parent=Msa1375990-mRNA-1
tig0019777	exon	65683	65784	65683	ID=Msa1375990-mRNA-1:exon:4809;Parent=Msa1375990-mRNA-1
tig0019777	exon	65868	66361	65868	ID=Msa1375990-mRNA-1:exon:4810;Parent=Msa1375990-mRNA-1
tig0019777	five_prime_UTR	64567	64651	64567	ID=Msa1375990-mRNA-1:five_prime_utr;Parent=Msa1375990-mRNA-1
tig0019777	CDS	64652	64737	64652	ID=Msa1375990-mRNA-1:cds;Parent=Msa1375990-mRNA-1
tig0019777	CDS	65683	65784	65683	ID=Msa1375990-mRNA-1:cds;Parent=Msa1375990-mRNA-1
tig0019777	CDS	65868	65889	65868	ID=Msa1375990-mRNA-1:cds;Parent=Msa1375990-mRNA-1
tig0019777	three_prime_UTR	65890	66361	65890	ID=Msa1375990-mRNA-1:three_prime_utr;Parent=Msa1375990-mRNA-1

Gene Sequence	>Msa1375990 ATGGGGCAGGAAAGCAAAGAGCCTTGCAAGAAAGAGGCTTGTAACATTCAAGCTTGCCTCTCCAAGAACAACTTTCTCCCGCAAAAGTGCCTTAAAGTTATTGAATTGCTGGACTCTTGCTGCTCAAGATGCGATTATAATTCAACTCACTGTGCTTCGCTCTCTGGACTATTGAAGCAAAATTCCAAATTTCAAGACTCGGCCAAGTAA
Protein sequence	>Msa1375990 MGQESKEPCKKEACNIQACLSKNNFLPQKCLKVIELLDSCCSRCDYNSTHCASLSGLLKQNSKFQDSAK*