AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1375990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1375990 MtrunA17_Chr4g0077101 97.101 69 2 0 1 69 1 69 9.85e-44 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1375990 AT1G09794.1 69.841 63 19 0 1 63 1 63 2.34e-27 94.0

Find 43 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGCAATTCAATAACTTTA+AGG 0.154340 tig0019777:-65686 None:intergenic
TTGAAGAAGGTTTGAGAAAA+TGG 0.165556 tig0019777:+64633 Msa1375990:exon
TCAAGACTCGGCCAAGTAAT+TGG 0.254409 tig0019777:+65871 Msa1375990:exon
CCTTAAAGTTATTGAATTGC+TGG 0.305156 tig0019777:+65686 Msa1375990:CDS
TGAAGAAGGTTTGAGAAAAT+GGG 0.324548 tig0019777:+64634 Msa1375990:exon
GAATCTCCCTACCCAGCATT+GGG 0.330066 tig0019777:-66011 None:intergenic
GAATCTCCCTACCCAGCATT+GGG 0.330066 tig0019777:-66187 None:intergenic
AAACTTAAATTCACAATTGC+TGG 0.339243 tig0019777:-66035 None:intergenic
AAACTTAAATTCACAATTGC+TGG 0.339243 tig0019777:-66211 None:intergenic
TTGCGGGAGAAAGTTGTTCT+TGG 0.356092 tig0019777:-64713 None:intergenic
GATAAAATTTAGATTAAGAA+AGG 0.388163 tig0019777:+65983 Msa1375990:three_prime_UTR
GATAAAATTTAGATTAAGAA+AGG 0.388163 tig0019777:+66159 Msa1375990:three_prime_UTR
ACTCACTGTGCTTCGCTCTC+TGG 0.391788 tig0019777:+65741 Msa1375990:CDS
GCTCACATCTACTGTGACTT+GGG 0.426926 tig0019777:-66089 None:intergenic
GCTCACATCTACTGTGACTT+GGG 0.426926 tig0019777:-66265 None:intergenic
TGCTCACATCTACTGTGACT+TGG 0.432516 tig0019777:-66090 None:intergenic
TGCTCACATCTACTGTGACT+TGG 0.432516 tig0019777:-66266 None:intergenic
CAACTACTTATCCAATTACT+TGG 0.432849 tig0019777:-65882 None:intergenic
ACACAAATATCAATTGCAAT+TGG 0.443864 tig0019777:-66308 None:intergenic
TGAATCTCCCTACCCAGCAT+TGG 0.444520 tig0019777:-66012 None:intergenic
TGAATCTCCCTACCCAGCAT+TGG 0.444520 tig0019777:-66188 None:intergenic
AGAAAGGGACACCCCAATGC+TGG 0.454087 tig0019777:+65999 Msa1375990:three_prime_UTR
AGAAAGGGACACCCCAATGC+TGG 0.454087 tig0019777:+66175 Msa1375990:three_prime_UTR
CTAATATGAAATTCCACAAT+CGG 0.475006 tig0019777:-66064 None:intergenic
CTAATATGAAATTCCACAAT+CGG 0.475006 tig0019777:-66240 None:intergenic
TGGATAAGTAGTTGAGGTGT+AGG 0.480749 tig0019777:+65891 Msa1375990:three_prime_UTR
CAAAGAGCCTTGCAAGAAAG+AGG 0.485000 tig0019777:+64666 Msa1375990:CDS
ATAAAATTTAGATTAAGAAA+GGG 0.500104 tig0019777:+65984 Msa1375990:three_prime_UTR
ATAAAATTTAGATTAAGAAA+GGG 0.500104 tig0019777:+66160 Msa1375990:three_prime_UTR
TTACAAGCCTCTTTCTTGCA+AGG 0.522587 tig0019777:-64673 None:intergenic
AAGGTTTGAGAAAATGGGGC+AGG 0.531461 tig0019777:+64639 Msa1375990:exon
GGACACCCCAATGCTGGGTA+GGG 0.550020 tig0019777:+66005 Msa1375990:three_prime_UTR
GGACACCCCAATGCTGGGTA+GGG 0.550020 tig0019777:+66181 Msa1375990:three_prime_UTR
GGGACACCCCAATGCTGGGT+AGG 0.554075 tig0019777:+66004 Msa1375990:three_prime_UTR
GGGACACCCCAATGCTGGGT+AGG 0.554075 tig0019777:+66180 Msa1375990:three_prime_UTR
GAAAGGGACACCCCAATGCT+GGG 0.575437 tig0019777:+66000 Msa1375990:three_prime_UTR
GAAAGGGACACCCCAATGCT+GGG 0.575437 tig0019777:+66176 Msa1375990:three_prime_UTR
GAAGAAGGTTTGAGAAAATG+GGG 0.579370 tig0019777:+64635 Msa1375990:exon
GGAGAAAGTTGTTCTTGGAG+AGG 0.604134 tig0019777:-64708 None:intergenic
TGTCTGCAGATTTCAAGACT+CGG 0.613114 tig0019777:+65859 Msa1375990:intron
AGTAATTGGATAAGTAGTTG+AGG 0.616983 tig0019777:+65885 Msa1375990:exon
AATCTCCCTACCCAGCATTG+GGG 0.629498 tig0019777:-66010 None:intergenic
AATCTCCCTACCCAGCATTG+GGG 0.629498 tig0019777:-66186 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTATAAGCAAATAT+TGG + tig0019777:65494-65513 Msa1375990:intron 10.0%
!! ATAAAATTTAGATTAAGAAA+GGG + tig0019777:65984-66003 Msa1375990:three_prime_UTR 10.0%
!! ATAAAATTTAGATTAAGAAA+GGG + tig0019777:65984-66003 Msa1375990:three_prime_UTR 10.0%
!!! ATTTTTTTATAAGCAAATAT+TGG + tig0019777:65494-65513 Msa1375990:intron 10.0%
!! ATAAAATTTAGATTAAGAAA+GGG + tig0019777:65984-66003 Msa1375990:three_prime_UTR 10.0%
!! ATAAAATTTAGATTAAGAAA+GGG + tig0019777:65984-66003 Msa1375990:three_prime_UTR 10.0%
!! AAAATCCCTAATAATATAAT+TGG + tig0019777:64838-64857 Msa1375990:intron 15.0%
!! TCAATCCAATTATATTATTA+GGG - tig0019777:64846-64865 None:intergenic 15.0%
!! TTCAATCCAATTATATTATT+AGG - tig0019777:64847-64866 None:intergenic 15.0%
!! AATTATATTAACACTAACAT+AGG - tig0019777:64915-64934 None:intergenic 15.0%
!! TATGTTAGTGTTAATATAAT+TGG + tig0019777:64914-64933 Msa1375990:intron 15.0%
!! ATGTTAGTGTTAATATAATT+GGG + tig0019777:64915-64934 Msa1375990:intron 15.0%
!! GATAAAATTTAGATTAAGAA+AGG + tig0019777:65983-66002 Msa1375990:three_prime_UTR 15.0%
!! GATAAAATTTAGATTAAGAA+AGG + tig0019777:65983-66002 Msa1375990:three_prime_UTR 15.0%
!! AAAATCCCTAATAATATAAT+TGG + tig0019777:64838-64857 Msa1375990:intron 15.0%
!! TCAATCCAATTATATTATTA+GGG - tig0019777:64846-64865 None:intergenic 15.0%
!! TTCAATCCAATTATATTATT+AGG - tig0019777:64847-64866 None:intergenic 15.0%
!! AATTATATTAACACTAACAT+AGG - tig0019777:64915-64934 None:intergenic 15.0%
!! TATGTTAGTGTTAATATAAT+TGG + tig0019777:64914-64933 Msa1375990:intron 15.0%
!! ATGTTAGTGTTAATATAATT+GGG + tig0019777:64915-64934 Msa1375990:intron 15.0%
!! GATAAAATTTAGATTAAGAA+AGG + tig0019777:65983-66002 Msa1375990:three_prime_UTR 15.0%
!! GATAAAATTTAGATTAAGAA+AGG + tig0019777:65983-66002 Msa1375990:three_prime_UTR 15.0%
!! CAAAACAAATCAAATGAAAT+AGG - tig0019777:64881-64900 None:intergenic 20.0%
!! TGACATAGTTAATTATAAGT+TGG + tig0019777:64988-65007 Msa1375990:intron 20.0%
!!! GTTTTTAATTAAAACAACCA+CGG + tig0019777:65176-65195 Msa1375990:intron 20.0%
!! CATAAATTAGTAGTATAAAG+AGG - tig0019777:65334-65353 None:intergenic 20.0%
!! AAAATTCCAGTTTCTAATAT+TGG - tig0019777:65479-65498 None:intergenic 20.0%
!!! TTAGTGGTTATGTTAATTTT+TGG + tig0019777:65521-65540 Msa1375990:intron 20.0%
!!! TTAATTTTTGGAATTGAACT+CGG + tig0019777:65533-65552 Msa1375990:intron 20.0%
!! CAAAACAAATCAAATGAAAT+AGG - tig0019777:64881-64900 None:intergenic 20.0%
!! TGACATAGTTAATTATAAGT+TGG + tig0019777:64988-65007 Msa1375990:intron 20.0%
!!! GTTTTTAATTAAAACAACCA+CGG + tig0019777:65176-65195 Msa1375990:intron 20.0%
!! CATAAATTAGTAGTATAAAG+AGG - tig0019777:65334-65353 None:intergenic 20.0%
!! AAAATTCCAGTTTCTAATAT+TGG - tig0019777:65479-65498 None:intergenic 20.0%
!!! TTAGTGGTTATGTTAATTTT+TGG + tig0019777:65521-65540 Msa1375990:intron 20.0%
!!! TTAATTTTTGGAATTGAACT+CGG + tig0019777:65533-65552 Msa1375990:intron 20.0%
! CGATAAAAAGATATTGAAGA+AGG + tig0019777:64620-64639 Msa1375990:five_prime_UTR 25.0%
!! AAACAAAAACATACTTTTGC+GGG - tig0019777:64732-64751 None:intergenic 25.0%
!! CAAACAAAAACATACTTTTG+CGG - tig0019777:64733-64752 None:intergenic 25.0%
! TATATCACAATCAAAATCAG+TGG - tig0019777:64814-64833 None:intergenic 25.0%
! TATTTAATTAACAGTCTGAC+AGG - tig0019777:64966-64985 None:intergenic 25.0%
!!! GTTTTCTAACTTTTCTAAGA+AGG - tig0019777:65124-65143 None:intergenic 25.0%
! TAAAGAATACTTGAACACTA+CGG + tig0019777:65254-65273 Msa1375990:intron 25.0%
!! AATGTTTTGTAAAGATAGCT+TGG - tig0019777:65606-65625 None:intergenic 25.0%
! AAGTTTCTTATAATACTGCT+AGG + tig0019777:65661-65680 Msa1375990:intron 25.0%
! AGTATAATTCATCACTTACT+TGG - tig0019777:65784-65803 None:intergenic 25.0%
! AAACTTAAATTCACAATTGC+TGG - tig0019777:66038-66057 None:intergenic 25.0%
! CTAATATGAAATTCCACAAT+CGG - tig0019777:66067-66086 None:intergenic 25.0%
! AAACTTAAATTCACAATTGC+TGG - tig0019777:66038-66057 None:intergenic 25.0%
! CTAATATGAAATTCCACAAT+CGG - tig0019777:66067-66086 None:intergenic 25.0%
! ACACAAATATCAATTGCAAT+TGG - tig0019777:66311-66330 None:intergenic 25.0%
! CGATAAAAAGATATTGAAGA+AGG + tig0019777:64620-64639 Msa1375990:five_prime_UTR 25.0%
!! AAACAAAAACATACTTTTGC+GGG - tig0019777:64732-64751 None:intergenic 25.0%
!! CAAACAAAAACATACTTTTG+CGG - tig0019777:64733-64752 None:intergenic 25.0%
! TATATCACAATCAAAATCAG+TGG - tig0019777:64814-64833 None:intergenic 25.0%
! TATTTAATTAACAGTCTGAC+AGG - tig0019777:64966-64985 None:intergenic 25.0%
!!! GTTTTCTAACTTTTCTAAGA+AGG - tig0019777:65124-65143 None:intergenic 25.0%
! TAAAGAATACTTGAACACTA+CGG + tig0019777:65254-65273 Msa1375990:intron 25.0%
!! AATGTTTTGTAAAGATAGCT+TGG - tig0019777:65606-65625 None:intergenic 25.0%
! AAGTTTCTTATAATACTGCT+AGG + tig0019777:65661-65680 Msa1375990:intron 25.0%
! AGTATAATTCATCACTTACT+TGG - tig0019777:65784-65803 None:intergenic 25.0%
! AAACTTAAATTCACAATTGC+TGG - tig0019777:66038-66057 None:intergenic 25.0%
! CTAATATGAAATTCCACAAT+CGG - tig0019777:66067-66086 None:intergenic 25.0%
! AAACTTAAATTCACAATTGC+TGG - tig0019777:66038-66057 None:intergenic 25.0%
! CTAATATGAAATTCCACAAT+CGG - tig0019777:66067-66086 None:intergenic 25.0%
! ACACAAATATCAATTGCAAT+TGG - tig0019777:66311-66330 None:intergenic 25.0%
TTGAAGAAGGTTTGAGAAAA+TGG + tig0019777:64633-64652 Msa1375990:exon 30.0%
TGAAGAAGGTTTGAGAAAAT+GGG + tig0019777:64634-64653 Msa1375990:exon 30.0%
AGTTAGAAAACTTTCTTCTG+CGG + tig0019777:65133-65152 Msa1375990:intron 30.0%
!! AGTTGTTTGTTTCTAACACA+AGG - tig0019777:65307-65326 None:intergenic 30.0%
AGCAAATATTGGTAAGTTAG+TGG + tig0019777:65505-65524 Msa1375990:intron 30.0%
! TTTTGTAAAGATAGCTTGGT+TGG - tig0019777:65602-65621 None:intergenic 30.0%
CATTGTTGAAACAAAACTGT+TGG + tig0019777:65622-65641 Msa1375990:intron 30.0%
CCAGCAATTCAATAACTTTA+AGG - tig0019777:65689-65708 None:intergenic 30.0%
CCTTAAAGTTATTGAATTGC+TGG + tig0019777:65686-65705 Msa1375990:CDS 30.0%
CAAAACAAAAATATGCAAGC+CGG - tig0019777:65841-65860 None:intergenic 30.0%
CAACTACTTATCCAATTACT+TGG - tig0019777:65885-65904 None:intergenic 30.0%
AGTAATTGGATAAGTAGTTG+AGG + tig0019777:65885-65904 Msa1375990:exon 30.0%
TTGAAGAAGGTTTGAGAAAA+TGG + tig0019777:64633-64652 Msa1375990:exon 30.0%
TGAAGAAGGTTTGAGAAAAT+GGG + tig0019777:64634-64653 Msa1375990:exon 30.0%
AGTTAGAAAACTTTCTTCTG+CGG + tig0019777:65133-65152 Msa1375990:intron 30.0%
!! AGTTGTTTGTTTCTAACACA+AGG - tig0019777:65307-65326 None:intergenic 30.0%
AGCAAATATTGGTAAGTTAG+TGG + tig0019777:65505-65524 Msa1375990:intron 30.0%
! TTTTGTAAAGATAGCTTGGT+TGG - tig0019777:65602-65621 None:intergenic 30.0%
CATTGTTGAAACAAAACTGT+TGG + tig0019777:65622-65641 Msa1375990:intron 30.0%
CCAGCAATTCAATAACTTTA+AGG - tig0019777:65689-65708 None:intergenic 30.0%
CCTTAAAGTTATTGAATTGC+TGG + tig0019777:65686-65705 Msa1375990:CDS 30.0%
CAAAACAAAAATATGCAAGC+CGG - tig0019777:65841-65860 None:intergenic 30.0%
CAACTACTTATCCAATTACT+TGG - tig0019777:65885-65904 None:intergenic 30.0%
AGTAATTGGATAAGTAGTTG+AGG + tig0019777:65885-65904 Msa1375990:exon 30.0%
GAAGAAGGTTTGAGAAAATG+GGG + tig0019777:64635-64654 Msa1375990:exon 35.0%
TCCACCATTAAACTCTATTC+CGG + tig0019777:65067-65086 Msa1375990:intron 35.0%
TATGCCGGAATAGAGTTTAA+TGG - tig0019777:65074-65093 None:intergenic 35.0%
AAAACCAAACACCTTTCATG+AGG - tig0019777:65161-65180 None:intergenic 35.0%
CTTCTGACATTTGCATCATT+TGG - tig0019777:65408-65427 None:intergenic 35.0%
CAAATGATGCAAATGTCAGA+AGG + tig0019777:65406-65425 Msa1375990:intron 35.0%
ACAACGCCAATATTAGAAAC+TGG + tig0019777:65470-65489 Msa1375990:intron 35.0%
!!! TTTTTGGAATTGAACTCGGA+AGG + tig0019777:65537-65556 Msa1375990:intron 35.0%
!!! TTTTGGAATTGAACTCGGAA+GGG + tig0019777:65538-65557 Msa1375990:intron 35.0%
TTCAGATTCATATCGATGAC+CGG + tig0019777:65819-65838 Msa1375990:intron 35.0%
!! TAAGTTTTGCTTACCGATTG+TGG + tig0019777:66051-66070 Msa1375990:three_prime_UTR 35.0%
!! TAAGTTTTGCTTACCGATTG+TGG + tig0019777:66051-66070 Msa1375990:three_prime_UTR 35.0%
GAAGAAGGTTTGAGAAAATG+GGG + tig0019777:64635-64654 Msa1375990:exon 35.0%
TCCACCATTAAACTCTATTC+CGG + tig0019777:65067-65086 Msa1375990:intron 35.0%
TATGCCGGAATAGAGTTTAA+TGG - tig0019777:65074-65093 None:intergenic 35.0%
AAAACCAAACACCTTTCATG+AGG - tig0019777:65161-65180 None:intergenic 35.0%
CTTCTGACATTTGCATCATT+TGG - tig0019777:65408-65427 None:intergenic 35.0%
CAAATGATGCAAATGTCAGA+AGG + tig0019777:65406-65425 Msa1375990:intron 35.0%
ACAACGCCAATATTAGAAAC+TGG + tig0019777:65470-65489 Msa1375990:intron 35.0%
!!! TTTTTGGAATTGAACTCGGA+AGG + tig0019777:65537-65556 Msa1375990:intron 35.0%
!!! TTTTGGAATTGAACTCGGAA+GGG + tig0019777:65538-65557 Msa1375990:intron 35.0%
TTCAGATTCATATCGATGAC+CGG + tig0019777:65819-65838 Msa1375990:intron 35.0%
!! TAAGTTTTGCTTACCGATTG+TGG + tig0019777:66051-66070 Msa1375990:three_prime_UTR 35.0%
!! TAAGTTTTGCTTACCGATTG+TGG + tig0019777:66051-66070 Msa1375990:three_prime_UTR 35.0%
TTACAAGCCTCTTTCTTGCA+AGG - tig0019777:64676-64695 None:intergenic 40.0%
AAAACTCTCTTCCTAGTGAC+TGG + tig0019777:65217-65236 Msa1375990:intron 40.0%
GCACAGTGGTATCATCTAAA+TGG + tig0019777:65430-65449 Msa1375990:intron 40.0%
TCTAAATGGCTATGCCTATG+CGG + tig0019777:65444-65463 Msa1375990:intron 40.0%
!! TAGCTTGGTTGGTGAGTTAA+GGG - tig0019777:65591-65610 None:intergenic 40.0%
!! ATAGCTTGGTTGGTGAGTTA+AGG - tig0019777:65592-65611 None:intergenic 40.0%
TGTCTGCAGATTTCAAGACT+CGG + tig0019777:65859-65878 Msa1375990:intron 40.0%
TGGATAAGTAGTTGAGGTGT+AGG + tig0019777:65891-65910 Msa1375990:three_prime_UTR 40.0%
TTACAAGCCTCTTTCTTGCA+AGG - tig0019777:64676-64695 None:intergenic 40.0%
AAAACTCTCTTCCTAGTGAC+TGG + tig0019777:65217-65236 Msa1375990:intron 40.0%
GCACAGTGGTATCATCTAAA+TGG + tig0019777:65430-65449 Msa1375990:intron 40.0%
TCTAAATGGCTATGCCTATG+CGG + tig0019777:65444-65463 Msa1375990:intron 40.0%
!! TAGCTTGGTTGGTGAGTTAA+GGG - tig0019777:65591-65610 None:intergenic 40.0%
!! ATAGCTTGGTTGGTGAGTTA+AGG - tig0019777:65592-65611 None:intergenic 40.0%
TGTCTGCAGATTTCAAGACT+CGG + tig0019777:65859-65878 Msa1375990:intron 40.0%
TGGATAAGTAGTTGAGGTGT+AGG + tig0019777:65891-65910 Msa1375990:three_prime_UTR 40.0%
AAGGTTTGAGAAAATGGGGC+AGG + tig0019777:64639-64658 Msa1375990:exon 45.0%
CAAAGAGCCTTGCAAGAAAG+AGG + tig0019777:64666-64685 Msa1375990:CDS 45.0%
GGAGAAAGTTGTTCTTGGAG+AGG - tig0019777:64711-64730 None:intergenic 45.0%
TTGCGGGAGAAAGTTGTTCT+TGG - tig0019777:64716-64735 None:intergenic 45.0%
ACAGTCTGACAGGAATGAAG+AGG - tig0019777:64956-64975 None:intergenic 45.0%
GTGGATATGCAGAAGCCATT+GGG - tig0019777:65052-65071 None:intergenic 45.0%
GCCGGAATAGAGTTTAATGG+TGG - tig0019777:65071-65090 None:intergenic 45.0%
! AAGGAGTGCGAAAACAGTCT+CGG - tig0019777:65105-65124 None:intergenic 45.0%
! AAATGTCAGAAGGTGCACAG+TGG + tig0019777:65416-65435 Msa1375990:intron 45.0%
TCAAGACTCGGCCAAGTAAT+TGG + tig0019777:65871-65890 Msa1375990:exon 45.0%
GCTCACATCTACTGTGACTT+GGG - tig0019777:66092-66111 None:intergenic 45.0%
TGCTCACATCTACTGTGACT+TGG - tig0019777:66093-66112 None:intergenic 45.0%
GCTCACATCTACTGTGACTT+GGG - tig0019777:66092-66111 None:intergenic 45.0%
TGCTCACATCTACTGTGACT+TGG - tig0019777:66093-66112 None:intergenic 45.0%
AAGGTTTGAGAAAATGGGGC+AGG + tig0019777:64639-64658 Msa1375990:exon 45.0%
CAAAGAGCCTTGCAAGAAAG+AGG + tig0019777:64666-64685 Msa1375990:CDS 45.0%
GGAGAAAGTTGTTCTTGGAG+AGG - tig0019777:64711-64730 None:intergenic 45.0%
TTGCGGGAGAAAGTTGTTCT+TGG - tig0019777:64716-64735 None:intergenic 45.0%
ACAGTCTGACAGGAATGAAG+AGG - tig0019777:64956-64975 None:intergenic 45.0%
GTGGATATGCAGAAGCCATT+GGG - tig0019777:65052-65071 None:intergenic 45.0%
GCCGGAATAGAGTTTAATGG+TGG - tig0019777:65071-65090 None:intergenic 45.0%
! AAGGAGTGCGAAAACAGTCT+CGG - tig0019777:65105-65124 None:intergenic 45.0%
! AAATGTCAGAAGGTGCACAG+TGG + tig0019777:65416-65435 Msa1375990:intron 45.0%
TCAAGACTCGGCCAAGTAAT+TGG + tig0019777:65871-65890 Msa1375990:exon 45.0%
GCTCACATCTACTGTGACTT+GGG - tig0019777:66092-66111 None:intergenic 45.0%
TGCTCACATCTACTGTGACT+TGG - tig0019777:66093-66112 None:intergenic 45.0%
GCTCACATCTACTGTGACTT+GGG - tig0019777:66092-66111 None:intergenic 45.0%
TGCTCACATCTACTGTGACT+TGG - tig0019777:66093-66112 None:intergenic 45.0%
GGTGGATATGCAGAAGCCAT+TGG - tig0019777:65053-65072 None:intergenic 50.0%
CTCTGATCTCACCAGTCACT+AGG - tig0019777:65231-65250 None:intergenic 50.0%
ATTGGCGTTGTGTTCCGCAT+AGG - tig0019777:65461-65480 None:intergenic 50.0%
AATCTCCCTACCCAGCATTG+GGG - tig0019777:66013-66032 None:intergenic 50.0%
GAATCTCCCTACCCAGCATT+GGG - tig0019777:66014-66033 None:intergenic 50.0%
TGAATCTCCCTACCCAGCAT+TGG - tig0019777:66015-66034 None:intergenic 50.0%
AATCTCCCTACCCAGCATTG+GGG - tig0019777:66013-66032 None:intergenic 50.0%
GAATCTCCCTACCCAGCATT+GGG - tig0019777:66014-66033 None:intergenic 50.0%
TGAATCTCCCTACCCAGCAT+TGG - tig0019777:66015-66034 None:intergenic 50.0%
GGTGGATATGCAGAAGCCAT+TGG - tig0019777:65053-65072 None:intergenic 50.0%
CTCTGATCTCACCAGTCACT+AGG - tig0019777:65231-65250 None:intergenic 50.0%
ATTGGCGTTGTGTTCCGCAT+AGG - tig0019777:65461-65480 None:intergenic 50.0%
AATCTCCCTACCCAGCATTG+GGG - tig0019777:66013-66032 None:intergenic 50.0%
GAATCTCCCTACCCAGCATT+GGG - tig0019777:66014-66033 None:intergenic 50.0%
TGAATCTCCCTACCCAGCAT+TGG - tig0019777:66015-66034 None:intergenic 50.0%
AATCTCCCTACCCAGCATTG+GGG - tig0019777:66013-66032 None:intergenic 50.0%
GAATCTCCCTACCCAGCATT+GGG - tig0019777:66014-66033 None:intergenic 50.0%
TGAATCTCCCTACCCAGCAT+TGG - tig0019777:66015-66034 None:intergenic 50.0%
TGTAACGCCCTCTGTCCCAA+TGG + tig0019777:65034-65053 Msa1375990:intron 55.0%
GCAGAAGCCATTGGGACAGA+GGG - tig0019777:65044-65063 None:intergenic 55.0%
TGCAGAAGCCATTGGGACAG+AGG - tig0019777:65045-65064 None:intergenic 55.0%
! GTCTCGGAGGTTGGTTATGC+CGG - tig0019777:65089-65108 None:intergenic 55.0%
GCGAAAACAGTCTCGGAGGT+TGG - tig0019777:65098-65117 None:intergenic 55.0%
GAGTGCGAAAACAGTCTCGG+AGG - tig0019777:65102-65121 None:intergenic 55.0%
!! GGCGCCTCATGAAAGGTGTT+TGG + tig0019777:65154-65173 Msa1375990:intron 55.0%
ACTCACTGTGCTTCGCTCTC+TGG + tig0019777:65741-65760 Msa1375990:CDS 55.0%
AGAAAGGGACACCCCAATGC+TGG + tig0019777:65999-66018 Msa1375990:three_prime_UTR 55.0%
GAAAGGGACACCCCAATGCT+GGG + tig0019777:66000-66019 Msa1375990:three_prime_UTR 55.0%
AGAAAGGGACACCCCAATGC+TGG + tig0019777:65999-66018 Msa1375990:three_prime_UTR 55.0%
GAAAGGGACACCCCAATGCT+GGG + tig0019777:66000-66019 Msa1375990:three_prime_UTR 55.0%
TGTAACGCCCTCTGTCCCAA+TGG + tig0019777:65034-65053 Msa1375990:intron 55.0%
GCAGAAGCCATTGGGACAGA+GGG - tig0019777:65044-65063 None:intergenic 55.0%
TGCAGAAGCCATTGGGACAG+AGG - tig0019777:65045-65064 None:intergenic 55.0%
! GTCTCGGAGGTTGGTTATGC+CGG - tig0019777:65089-65108 None:intergenic 55.0%
GCGAAAACAGTCTCGGAGGT+TGG - tig0019777:65098-65117 None:intergenic 55.0%
GAGTGCGAAAACAGTCTCGG+AGG - tig0019777:65102-65121 None:intergenic 55.0%
!! GGCGCCTCATGAAAGGTGTT+TGG + tig0019777:65154-65173 Msa1375990:intron 55.0%
ACTCACTGTGCTTCGCTCTC+TGG + tig0019777:65741-65760 Msa1375990:CDS 55.0%
AGAAAGGGACACCCCAATGC+TGG + tig0019777:65999-66018 Msa1375990:three_prime_UTR 55.0%
GAAAGGGACACCCCAATGCT+GGG + tig0019777:66000-66019 Msa1375990:three_prime_UTR 55.0%
AGAAAGGGACACCCCAATGC+TGG + tig0019777:65999-66018 Msa1375990:three_prime_UTR 55.0%
GAAAGGGACACCCCAATGCT+GGG + tig0019777:66000-66019 Msa1375990:three_prime_UTR 55.0%
CTTCTGCGGCGCCTCATGAA+AGG + tig0019777:65147-65166 Msa1375990:intron 60.0%
CAGCGCAACTGTGATGTCCG+TGG - tig0019777:65196-65215 None:intergenic 60.0%
GGACACCCCAATGCTGGGTA+GGG + tig0019777:66005-66024 Msa1375990:three_prime_UTR 60.0%
GGACACCCCAATGCTGGGTA+GGG + tig0019777:66005-66024 Msa1375990:three_prime_UTR 60.0%
CTTCTGCGGCGCCTCATGAA+AGG + tig0019777:65147-65166 Msa1375990:intron 60.0%
CAGCGCAACTGTGATGTCCG+TGG - tig0019777:65196-65215 None:intergenic 60.0%
GGACACCCCAATGCTGGGTA+GGG + tig0019777:66005-66024 Msa1375990:three_prime_UTR 60.0%
GGACACCCCAATGCTGGGTA+GGG + tig0019777:66005-66024 Msa1375990:three_prime_UTR 60.0%
GGGACACCCCAATGCTGGGT+AGG + tig0019777:66004-66023 Msa1375990:three_prime_UTR 65.0%
GGGACACCCCAATGCTGGGT+AGG + tig0019777:66004-66023 Msa1375990:three_prime_UTR 65.0%
GGGACACCCCAATGCTGGGT+AGG + tig0019777:66004-66023 Msa1375990:three_prime_UTR 65.0%
GGGACACCCCAATGCTGGGT+AGG + tig0019777:66004-66023 Msa1375990:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0019777 gene 64567 66361 64567 ID=Msa1375990;Name=Msa1375990
tig0019777 mRNA 64567 66361 64567 ID=Msa1375990-mRNA-1;Parent=Msa1375990;Name=Msa1375990-mRNA-1;_AED=0.09;_eAED=0.09;_QI=85|1|1|1|1|1|3|472|69
tig0019777 exon 64567 64737 64567 ID=Msa1375990-mRNA-1:exon:4808;Parent=Msa1375990-mRNA-1
tig0019777 exon 65683 65784 65683 ID=Msa1375990-mRNA-1:exon:4809;Parent=Msa1375990-mRNA-1
tig0019777 exon 65868 66361 65868 ID=Msa1375990-mRNA-1:exon:4810;Parent=Msa1375990-mRNA-1
tig0019777 five_prime_UTR 64567 64651 64567 ID=Msa1375990-mRNA-1:five_prime_utr;Parent=Msa1375990-mRNA-1
tig0019777 CDS 64652 64737 64652 ID=Msa1375990-mRNA-1:cds;Parent=Msa1375990-mRNA-1
tig0019777 CDS 65683 65784 65683 ID=Msa1375990-mRNA-1:cds;Parent=Msa1375990-mRNA-1
tig0019777 CDS 65868 65889 65868 ID=Msa1375990-mRNA-1:cds;Parent=Msa1375990-mRNA-1
tig0019777 three_prime_UTR 65890 66361 65890 ID=Msa1375990-mRNA-1:three_prime_utr;Parent=Msa1375990-mRNA-1
Gene Sequence

>Msa1375990

ATGGGGCAGGAAAGCAAAGAGCCTTGCAAGAAAGAGGCTTGTAACATTCAAGCTTGCCTCTCCAAGAACAACTTTCTCCCGCAAAAGTGCCTTAAAGTTATTGAATTGCTGGACTCTTGCTGCTCAAGATGCGATTATAATTCAACTCACTGTGCTTCGCTCTCTGGACTATTGAAGCAAAATTCCAAATTTCAAGACTCGGCCAAGTAA
Protein sequence

>Msa1375990

MGQESKEPCKKEACNIQACLSKNNFLPQKCLKVIELLDSCCSRCDYNSTHCASLSGLLKQNSKFQDSAK*