AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1403800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1403800 MtrunA17_Chr4g0077101 97.101 69 2 0 1 69 1 69 9.85e-44 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1403800 AT1G09794.1 69.841 63 19 0 1 63 1 63 2.34e-27 94.0

Find 31 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGCAATTCAATAACTTTA+AGG 0.154340 tig0022980:+21014 None:intergenic
TTGAAGAAGGTTTGAGAAAA+TGG 0.165556 tig0022980:-22023 Msa1403800:exon
TCAAGACTCGGCCAAGTAAT+TGG 0.254409 tig0022980:-20829 Msa1403800:exon
CCTTAAAGTTATTGAATTGC+TGG 0.305156 tig0022980:-21014 Msa1403800:CDS
TGAAGAAGGTTTGAGAAAAT+GGG 0.324548 tig0022980:-22022 Msa1403800:exon
GAATCTCCCTACCCAGCATT+GGG 0.330066 tig0022980:+20689 None:intergenic
AAACTTAAATTCACAATTGC+TGG 0.339243 tig0022980:+20665 None:intergenic
TTGCGGGAGAAAGTTGTTCT+TGG 0.356092 tig0022980:+21943 None:intergenic
GATAAAATTTAGATTAAGAA+AGG 0.388163 tig0022980:-20717 Msa1403800:three_prime_UTR
ACTCACTGTGCTTCGCTCTC+TGG 0.391788 tig0022980:-20959 Msa1403800:CDS
GCTCACATCTACTGTGACTT+GGG 0.426926 tig0022980:+20611 None:intergenic
TGCTCACATCTACTGTGACT+TGG 0.432516 tig0022980:+20610 None:intergenic
CAACTACTTATCCAATTACT+TGG 0.432849 tig0022980:+20818 None:intergenic
ACACAAATATCAATTGCAAT+TGG 0.443864 tig0022980:+20568 None:intergenic
TGAATCTCCCTACCCAGCAT+TGG 0.444520 tig0022980:+20688 None:intergenic
AGAAAGGGACACCCCAATGC+TGG 0.454087 tig0022980:-20701 Msa1403800:three_prime_UTR
TGGATAAGTAGTTGAGGTGT+AGG 0.458431 tig0022980:-20809 Msa1403800:three_prime_UTR
CTAATATGAAATTCCACAAT+CGG 0.475006 tig0022980:+20636 None:intergenic
CAAAGAGCCTTGCAAGAAAG+AGG 0.485000 tig0022980:-21990 Msa1403800:CDS
ATAAAATTTAGATTAAGAAA+GGG 0.500104 tig0022980:-20716 Msa1403800:three_prime_UTR
TTACAAGCCTCTTTCTTGCA+AGG 0.522587 tig0022980:+21983 None:intergenic
AAGGTTTGAGAAAATGGGGC+AGG 0.531461 tig0022980:-22017 Msa1403800:exon
GGACACCCCAATGCTGGGTA+GGG 0.550020 tig0022980:-20695 Msa1403800:three_prime_UTR
GGGACACCCCAATGCTGGGT+AGG 0.554075 tig0022980:-20696 Msa1403800:three_prime_UTR
GGATAAGTAGTTGAGGTGTA+GGG 0.557175 tig0022980:-20808 Msa1403800:three_prime_UTR
GAAAGGGACACCCCAATGCT+GGG 0.575437 tig0022980:-20700 Msa1403800:three_prime_UTR
GAAGAAGGTTTGAGAAAATG+GGG 0.579370 tig0022980:-22021 Msa1403800:exon
GGAGAAAGTTGTTCTTGGAG+AGG 0.604134 tig0022980:+21948 None:intergenic
TGTCTGCAGATTTCAAGACT+CGG 0.613114 tig0022980:-20841 Msa1403800:intron
AGTAATTGGATAAGTAGTTG+AGG 0.616983 tig0022980:-20815 Msa1403800:exon
AATCTCCCTACCCAGCATTG+GGG 0.629498 tig0022980:+20690 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTATAAGCAAATAT+TGG - tig0022980:21427-21446 Msa1403800:intron 10.0%
!! ATAAAATTTAGATTAAGAAA+GGG - tig0022980:21917-21936 Msa1403800:intron 10.0%
!!! ATTTTTTTATAAGCAAATAT+TGG - tig0022980:21427-21446 Msa1403800:intron 10.0%
!! ATAAAATTTAGATTAAGAAA+GGG - tig0022980:21917-21936 Msa1403800:intron 10.0%
!! AAAATCCCTAATAATATAAT+TGG - tig0022980:20815-20834 Msa1403800:exon 15.0%
!! TCAATCCAATTATATTATTA+GGG + tig0022980:20823-20842 None:intergenic 15.0%
!! TTCAATCCAATTATATTATT+AGG + tig0022980:20824-20843 None:intergenic 15.0%
!! AATTATATTAACACTAACAT+AGG + tig0022980:20892-20911 None:intergenic 15.0%
!! TATGTTAGTGTTAATATAAT+TGG - tig0022980:20891-20910 Msa1403800:intron 15.0%
!! ATGTTAGTGTTAATATAATT+GGG - tig0022980:20892-20911 Msa1403800:intron 15.0%
!! GATAAAATTTAGATTAAGAA+AGG - tig0022980:21916-21935 Msa1403800:intron 15.0%
!! AAAATCCCTAATAATATAAT+TGG - tig0022980:20815-20834 Msa1403800:exon 15.0%
!! TCAATCCAATTATATTATTA+GGG + tig0022980:20823-20842 None:intergenic 15.0%
!! TTCAATCCAATTATATTATT+AGG + tig0022980:20824-20843 None:intergenic 15.0%
!! AATTATATTAACACTAACAT+AGG + tig0022980:20892-20911 None:intergenic 15.0%
!! TATGTTAGTGTTAATATAAT+TGG - tig0022980:20891-20910 Msa1403800:intron 15.0%
!! ATGTTAGTGTTAATATAATT+GGG - tig0022980:20892-20911 Msa1403800:intron 15.0%
!! GATAAAATTTAGATTAAGAA+AGG - tig0022980:21916-21935 Msa1403800:intron 15.0%
!! CAAAACAAATCAAATGAAAT+AGG + tig0022980:20858-20877 None:intergenic 20.0%
!! TGACATAGTTAATTATAAGT+TGG - tig0022980:20965-20984 Msa1403800:CDS 20.0%
!!! GTTTTTAATTAAAACAACCA+CGG - tig0022980:21153-21172 Msa1403800:intron 20.0%
!! AAAATTCCAGTTTCTAATAT+TGG + tig0022980:21412-21431 None:intergenic 20.0%
!!! TTAGTGGTTATGTTAATTTT+TGG - tig0022980:21454-21473 Msa1403800:intron 20.0%
!!! TTAATTTTTGGAATTGAACT+CGG - tig0022980:21466-21485 Msa1403800:intron 20.0%
!! CAAAACAAATCAAATGAAAT+AGG + tig0022980:20858-20877 None:intergenic 20.0%
!! TGACATAGTTAATTATAAGT+TGG - tig0022980:20965-20984 Msa1403800:CDS 20.0%
!!! GTTTTTAATTAAAACAACCA+CGG - tig0022980:21153-21172 Msa1403800:intron 20.0%
!! AAAATTCCAGTTTCTAATAT+TGG + tig0022980:21412-21431 None:intergenic 20.0%
!!! TTAGTGGTTATGTTAATTTT+TGG - tig0022980:21454-21473 Msa1403800:intron 20.0%
!!! TTAATTTTTGGAATTGAACT+CGG - tig0022980:21466-21485 Msa1403800:intron 20.0%
! CGATAAAAAGATATTGAAGA+AGG - tig0022980:20597-20616 Msa1403800:three_prime_UTR 25.0%
!! AAACAAAAACATACTTTTGC+GGG + tig0022980:20709-20728 None:intergenic 25.0%
!! CAAACAAAAACATACTTTTG+CGG + tig0022980:20710-20729 None:intergenic 25.0%
! TATATCACAATCAAAATCAG+TGG + tig0022980:20791-20810 None:intergenic 25.0%
! TATTTAATTAACAGTCTGAC+AGG + tig0022980:20943-20962 None:intergenic 25.0%
!!! GTTTTCTAACTTTTCTAAGA+AGG + tig0022980:21101-21120 None:intergenic 25.0%
! TAAAGAATACTTGAACACTA+CGG - tig0022980:21231-21250 Msa1403800:intron 25.0%
!! AATGTTTTGTAAAGATAGCT+TGG + tig0022980:21539-21558 None:intergenic 25.0%
! AAGTTTCTTATAATACTGCT+AGG - tig0022980:21594-21613 Msa1403800:intron 25.0%
! AGTATAATTCATCACTTACT+TGG + tig0022980:21717-21736 None:intergenic 25.0%
! AAACTTAAATTCACAATTGC+TGG + tig0022980:21971-21990 None:intergenic 25.0%
! CTAATATGAAATTCCACAAT+CGG + tig0022980:22000-22019 None:intergenic 25.0%
! ACACAAATATCAATTGCAAT+TGG + tig0022980:22068-22087 None:intergenic 25.0%
! CGATAAAAAGATATTGAAGA+AGG - tig0022980:20597-20616 Msa1403800:three_prime_UTR 25.0%
!! AAACAAAAACATACTTTTGC+GGG + tig0022980:20709-20728 None:intergenic 25.0%
!! CAAACAAAAACATACTTTTG+CGG + tig0022980:20710-20729 None:intergenic 25.0%
! TATATCACAATCAAAATCAG+TGG + tig0022980:20791-20810 None:intergenic 25.0%
! TATTTAATTAACAGTCTGAC+AGG + tig0022980:20943-20962 None:intergenic 25.0%
!!! GTTTTCTAACTTTTCTAAGA+AGG + tig0022980:21101-21120 None:intergenic 25.0%
! TAAAGAATACTTGAACACTA+CGG - tig0022980:21231-21250 Msa1403800:intron 25.0%
!! AATGTTTTGTAAAGATAGCT+TGG + tig0022980:21539-21558 None:intergenic 25.0%
! AAGTTTCTTATAATACTGCT+AGG - tig0022980:21594-21613 Msa1403800:intron 25.0%
! AGTATAATTCATCACTTACT+TGG + tig0022980:21717-21736 None:intergenic 25.0%
! AAACTTAAATTCACAATTGC+TGG + tig0022980:21971-21990 None:intergenic 25.0%
! CTAATATGAAATTCCACAAT+CGG + tig0022980:22000-22019 None:intergenic 25.0%
! ACACAAATATCAATTGCAAT+TGG + tig0022980:22068-22087 None:intergenic 25.0%
TTGAAGAAGGTTTGAGAAAA+TGG - tig0022980:20610-20629 Msa1403800:three_prime_UTR 30.0%
TGAAGAAGGTTTGAGAAAAT+GGG - tig0022980:20611-20630 Msa1403800:three_prime_UTR 30.0%
AGTTAGAAAACTTTCTTCTG+CGG - tig0022980:21110-21129 Msa1403800:intron 30.0%
AGCAAATATTGGTAAGTTAG+TGG - tig0022980:21438-21457 Msa1403800:intron 30.0%
! TTTTGTAAAGATAGCTTGGT+TGG + tig0022980:21535-21554 None:intergenic 30.0%
CATTGTTGAAACAAAACTGT+TGG - tig0022980:21555-21574 Msa1403800:intron 30.0%
CCAGCAATTCAATAACTTTA+AGG + tig0022980:21622-21641 None:intergenic 30.0%
CCTTAAAGTTATTGAATTGC+TGG - tig0022980:21619-21638 Msa1403800:intron 30.0%
CAAAACAAAAATATGCAAGC+CGG + tig0022980:21774-21793 None:intergenic 30.0%
CAACTACTTATCCAATTACT+TGG + tig0022980:21818-21837 None:intergenic 30.0%
AGTAATTGGATAAGTAGTTG+AGG - tig0022980:21818-21837 Msa1403800:intron 30.0%
TTGAAGAAGGTTTGAGAAAA+TGG - tig0022980:20610-20629 Msa1403800:three_prime_UTR 30.0%
TGAAGAAGGTTTGAGAAAAT+GGG - tig0022980:20611-20630 Msa1403800:three_prime_UTR 30.0%
AGTTAGAAAACTTTCTTCTG+CGG - tig0022980:21110-21129 Msa1403800:intron 30.0%
AGCAAATATTGGTAAGTTAG+TGG - tig0022980:21438-21457 Msa1403800:intron 30.0%
! TTTTGTAAAGATAGCTTGGT+TGG + tig0022980:21535-21554 None:intergenic 30.0%
CATTGTTGAAACAAAACTGT+TGG - tig0022980:21555-21574 Msa1403800:intron 30.0%
CCAGCAATTCAATAACTTTA+AGG + tig0022980:21622-21641 None:intergenic 30.0%
CCTTAAAGTTATTGAATTGC+TGG - tig0022980:21619-21638 Msa1403800:intron 30.0%
CAAAACAAAAATATGCAAGC+CGG + tig0022980:21774-21793 None:intergenic 30.0%
CAACTACTTATCCAATTACT+TGG + tig0022980:21818-21837 None:intergenic 30.0%
AGTAATTGGATAAGTAGTTG+AGG - tig0022980:21818-21837 Msa1403800:intron 30.0%
GAAGAAGGTTTGAGAAAATG+GGG - tig0022980:20612-20631 Msa1403800:three_prime_UTR 35.0%
TCCACCATTAAACTCTATTC+CGG - tig0022980:21044-21063 Msa1403800:intron 35.0%
TATGCCGGAATAGAGTTTAA+TGG + tig0022980:21051-21070 None:intergenic 35.0%
AAAACCAAACACCTTTCATG+AGG + tig0022980:21138-21157 None:intergenic 35.0%
CTTCTGACATTTGCATCATT+TGG + tig0022980:21341-21360 None:intergenic 35.0%
CAAATGATGCAAATGTCAGA+AGG - tig0022980:21339-21358 Msa1403800:intron 35.0%
ACAACGCCAATATTAGAAAC+TGG - tig0022980:21403-21422 Msa1403800:intron 35.0%
!!! TTTTTGGAATTGAACTCGGA+AGG - tig0022980:21470-21489 Msa1403800:intron 35.0%
!!! TTTTGGAATTGAACTCGGAA+GGG - tig0022980:21471-21490 Msa1403800:intron 35.0%
TTCAGATTCATATCGATGAC+CGG - tig0022980:21752-21771 Msa1403800:intron 35.0%
!! TAAGTTTTGCTTACCGATTG+TGG - tig0022980:21984-22003 Msa1403800:CDS 35.0%
GAAGAAGGTTTGAGAAAATG+GGG - tig0022980:20612-20631 Msa1403800:three_prime_UTR 35.0%
TCCACCATTAAACTCTATTC+CGG - tig0022980:21044-21063 Msa1403800:intron 35.0%
TATGCCGGAATAGAGTTTAA+TGG + tig0022980:21051-21070 None:intergenic 35.0%
AAAACCAAACACCTTTCATG+AGG + tig0022980:21138-21157 None:intergenic 35.0%
CTTCTGACATTTGCATCATT+TGG + tig0022980:21341-21360 None:intergenic 35.0%
CAAATGATGCAAATGTCAGA+AGG - tig0022980:21339-21358 Msa1403800:intron 35.0%
ACAACGCCAATATTAGAAAC+TGG - tig0022980:21403-21422 Msa1403800:intron 35.0%
!!! TTTTTGGAATTGAACTCGGA+AGG - tig0022980:21470-21489 Msa1403800:intron 35.0%
!!! TTTTGGAATTGAACTCGGAA+GGG - tig0022980:21471-21490 Msa1403800:intron 35.0%
TTCAGATTCATATCGATGAC+CGG - tig0022980:21752-21771 Msa1403800:intron 35.0%
!! TAAGTTTTGCTTACCGATTG+TGG - tig0022980:21984-22003 Msa1403800:CDS 35.0%
TTACAAGCCTCTTTCTTGCA+AGG + tig0022980:20653-20672 None:intergenic 40.0%
GCACAGTGGTATCATCTAAA+TGG - tig0022980:21363-21382 Msa1403800:intron 40.0%
TCTAAATGGCTATGCCTATG+CGG - tig0022980:21377-21396 Msa1403800:intron 40.0%
!! TAGCTTGGTTGGTGAGTTAA+GGG + tig0022980:21524-21543 None:intergenic 40.0%
!! ATAGCTTGGTTGGTGAGTTA+AGG + tig0022980:21525-21544 None:intergenic 40.0%
TGTCTGCAGATTTCAAGACT+CGG - tig0022980:21792-21811 Msa1403800:intron 40.0%
TGGATAAGTAGTTGAGGTGT+AGG - tig0022980:21824-21843 Msa1403800:intron 40.0%
GGATAAGTAGTTGAGGTGTA+GGG - tig0022980:21825-21844 Msa1403800:intron 40.0%
TTACAAGCCTCTTTCTTGCA+AGG + tig0022980:20653-20672 None:intergenic 40.0%
GCACAGTGGTATCATCTAAA+TGG - tig0022980:21363-21382 Msa1403800:intron 40.0%
TCTAAATGGCTATGCCTATG+CGG - tig0022980:21377-21396 Msa1403800:intron 40.0%
!! TAGCTTGGTTGGTGAGTTAA+GGG + tig0022980:21524-21543 None:intergenic 40.0%
!! ATAGCTTGGTTGGTGAGTTA+AGG + tig0022980:21525-21544 None:intergenic 40.0%
TGTCTGCAGATTTCAAGACT+CGG - tig0022980:21792-21811 Msa1403800:intron 40.0%
TGGATAAGTAGTTGAGGTGT+AGG - tig0022980:21824-21843 Msa1403800:intron 40.0%
GGATAAGTAGTTGAGGTGTA+GGG - tig0022980:21825-21844 Msa1403800:intron 40.0%
AAGGTTTGAGAAAATGGGGC+AGG - tig0022980:20616-20635 Msa1403800:three_prime_UTR 45.0%
CAAAGAGCCTTGCAAGAAAG+AGG - tig0022980:20643-20662 Msa1403800:three_prime_UTR 45.0%
GGAGAAAGTTGTTCTTGGAG+AGG + tig0022980:20688-20707 None:intergenic 45.0%
TTGCGGGAGAAAGTTGTTCT+TGG + tig0022980:20693-20712 None:intergenic 45.0%
ACAGTCTGACAGGAATGAAG+AGG + tig0022980:20933-20952 None:intergenic 45.0%
GTGGATATGCAGAAGCCATT+GGG + tig0022980:21029-21048 None:intergenic 45.0%
GCCGGAATAGAGTTTAATGG+TGG + tig0022980:21048-21067 None:intergenic 45.0%
! AAGGAGTGCGAAAACAGTCT+CGG + tig0022980:21082-21101 None:intergenic 45.0%
AAAACTCTCTTCCTGGTGAC+TGG - tig0022980:21194-21213 Msa1403800:intron 45.0%
! AAATGTCAGAAGGTGCACAG+TGG - tig0022980:21349-21368 Msa1403800:intron 45.0%
TCAAGACTCGGCCAAGTAAT+TGG - tig0022980:21804-21823 Msa1403800:intron 45.0%
GCTCACATCTACTGTGACTT+GGG + tig0022980:22025-22044 None:intergenic 45.0%
TGCTCACATCTACTGTGACT+TGG + tig0022980:22026-22045 None:intergenic 45.0%
AAGGTTTGAGAAAATGGGGC+AGG - tig0022980:20616-20635 Msa1403800:three_prime_UTR 45.0%
CAAAGAGCCTTGCAAGAAAG+AGG - tig0022980:20643-20662 Msa1403800:three_prime_UTR 45.0%
GGAGAAAGTTGTTCTTGGAG+AGG + tig0022980:20688-20707 None:intergenic 45.0%
TTGCGGGAGAAAGTTGTTCT+TGG + tig0022980:20693-20712 None:intergenic 45.0%
ACAGTCTGACAGGAATGAAG+AGG + tig0022980:20933-20952 None:intergenic 45.0%
GTGGATATGCAGAAGCCATT+GGG + tig0022980:21029-21048 None:intergenic 45.0%
GCCGGAATAGAGTTTAATGG+TGG + tig0022980:21048-21067 None:intergenic 45.0%
! AAGGAGTGCGAAAACAGTCT+CGG + tig0022980:21082-21101 None:intergenic 45.0%
AAAACTCTCTTCCTGGTGAC+TGG - tig0022980:21194-21213 Msa1403800:intron 45.0%
! AAATGTCAGAAGGTGCACAG+TGG - tig0022980:21349-21368 Msa1403800:intron 45.0%
TCAAGACTCGGCCAAGTAAT+TGG - tig0022980:21804-21823 Msa1403800:intron 45.0%
GCTCACATCTACTGTGACTT+GGG + tig0022980:22025-22044 None:intergenic 45.0%
TGCTCACATCTACTGTGACT+TGG + tig0022980:22026-22045 None:intergenic 45.0%
GGTGGATATGCAGAAGCCAT+TGG + tig0022980:21030-21049 None:intergenic 50.0%
GCGCTGAAAAACTCTCTTCC+TGG - tig0022980:21187-21206 Msa1403800:intron 50.0%
ATTGGCGTTGTGTTCCGCAT+AGG + tig0022980:21394-21413 None:intergenic 50.0%
AATCTCCCTACCCAGCATTG+GGG + tig0022980:21946-21965 None:intergenic 50.0%
GAATCTCCCTACCCAGCATT+GGG + tig0022980:21947-21966 None:intergenic 50.0%
TGAATCTCCCTACCCAGCAT+TGG + tig0022980:21948-21967 None:intergenic 50.0%
GGTGGATATGCAGAAGCCAT+TGG + tig0022980:21030-21049 None:intergenic 50.0%
GCGCTGAAAAACTCTCTTCC+TGG - tig0022980:21187-21206 Msa1403800:intron 50.0%
ATTGGCGTTGTGTTCCGCAT+AGG + tig0022980:21394-21413 None:intergenic 50.0%
AATCTCCCTACCCAGCATTG+GGG + tig0022980:21946-21965 None:intergenic 50.0%
GAATCTCCCTACCCAGCATT+GGG + tig0022980:21947-21966 None:intergenic 50.0%
TGAATCTCCCTACCCAGCAT+TGG + tig0022980:21948-21967 None:intergenic 50.0%
TGTAACGCCCTCTGTCCCAA+TGG - tig0022980:21011-21030 Msa1403800:CDS 55.0%
GCAGAAGCCATTGGGACAGA+GGG + tig0022980:21021-21040 None:intergenic 55.0%
TGCAGAAGCCATTGGGACAG+AGG + tig0022980:21022-21041 None:intergenic 55.0%
! GTCTCGGAGGTTGGTTATGC+CGG + tig0022980:21066-21085 None:intergenic 55.0%
GCGAAAACAGTCTCGGAGGT+TGG + tig0022980:21075-21094 None:intergenic 55.0%
GAGTGCGAAAACAGTCTCGG+AGG + tig0022980:21079-21098 None:intergenic 55.0%
!! GGCGCCTCATGAAAGGTGTT+TGG - tig0022980:21131-21150 Msa1403800:intron 55.0%
CTCTGATCTCACCAGTCACC+AGG + tig0022980:21208-21227 None:intergenic 55.0%
ACTCACTGTGCTTCGCTCTC+TGG - tig0022980:21674-21693 Msa1403800:intron 55.0%
AGAAAGGGACACCCCAATGC+TGG - tig0022980:21932-21951 Msa1403800:intron 55.0%
GAAAGGGACACCCCAATGCT+GGG - tig0022980:21933-21952 Msa1403800:intron 55.0%
TGTAACGCCCTCTGTCCCAA+TGG - tig0022980:21011-21030 Msa1403800:CDS 55.0%
GCAGAAGCCATTGGGACAGA+GGG + tig0022980:21021-21040 None:intergenic 55.0%
TGCAGAAGCCATTGGGACAG+AGG + tig0022980:21022-21041 None:intergenic 55.0%
! GTCTCGGAGGTTGGTTATGC+CGG + tig0022980:21066-21085 None:intergenic 55.0%
GCGAAAACAGTCTCGGAGGT+TGG + tig0022980:21075-21094 None:intergenic 55.0%
GAGTGCGAAAACAGTCTCGG+AGG + tig0022980:21079-21098 None:intergenic 55.0%
!! GGCGCCTCATGAAAGGTGTT+TGG - tig0022980:21131-21150 Msa1403800:intron 55.0%
CTCTGATCTCACCAGTCACC+AGG + tig0022980:21208-21227 None:intergenic 55.0%
ACTCACTGTGCTTCGCTCTC+TGG - tig0022980:21674-21693 Msa1403800:intron 55.0%
AGAAAGGGACACCCCAATGC+TGG - tig0022980:21932-21951 Msa1403800:intron 55.0%
GAAAGGGACACCCCAATGCT+GGG - tig0022980:21933-21952 Msa1403800:intron 55.0%
CTTCTGCGGCGCCTCATGAA+AGG - tig0022980:21124-21143 Msa1403800:intron 60.0%
CAGCGCAACTGTGATGTCCG+TGG + tig0022980:21173-21192 None:intergenic 60.0%
GGACACCCCAATGCTGGGTA+GGG - tig0022980:21938-21957 Msa1403800:intron 60.0%
CTTCTGCGGCGCCTCATGAA+AGG - tig0022980:21124-21143 Msa1403800:intron 60.0%
CAGCGCAACTGTGATGTCCG+TGG + tig0022980:21173-21192 None:intergenic 60.0%
GGACACCCCAATGCTGGGTA+GGG - tig0022980:21938-21957 Msa1403800:intron 60.0%
GGGACACCCCAATGCTGGGT+AGG - tig0022980:21937-21956 Msa1403800:intron 65.0%
GGGACACCCCAATGCTGGGT+AGG - tig0022980:21937-21956 Msa1403800:intron 65.0%
Chromosome Type Strat End Strand Name
tig0022980 gene 20544 22111 20544 ID=Msa1403800;Name=Msa1403800
tig0022980 mRNA 20544 22111 20544 ID=Msa1403800-mRNA-1;Parent=Msa1403800;Name=Msa1403800-mRNA-1;_AED=0.30;_eAED=0.30;_QI=85|1|1|1|1|1|3|289|69
tig0022980 exon 20544 20854 20544 ID=Msa1403800-mRNA-1:exon:19498;Parent=Msa1403800-mRNA-1
tig0022980 exon 20938 21039 20938 ID=Msa1403800-mRNA-1:exon:19497;Parent=Msa1403800-mRNA-1
tig0022980 exon 21941 22111 21941 ID=Msa1403800-mRNA-1:exon:19496;Parent=Msa1403800-mRNA-1
tig0022980 five_prime_UTR 22027 22111 22027 ID=Msa1403800-mRNA-1:five_prime_utr;Parent=Msa1403800-mRNA-1
tig0022980 CDS 21941 22026 21941 ID=Msa1403800-mRNA-1:cds;Parent=Msa1403800-mRNA-1
tig0022980 CDS 20938 21039 20938 ID=Msa1403800-mRNA-1:cds;Parent=Msa1403800-mRNA-1
tig0022980 CDS 20833 20854 20833 ID=Msa1403800-mRNA-1:cds;Parent=Msa1403800-mRNA-1
tig0022980 three_prime_UTR 20544 20832 20544 ID=Msa1403800-mRNA-1:three_prime_utr;Parent=Msa1403800-mRNA-1
Gene Sequence

>Msa1403800

ATGGGGCAGGAAAGCAAAGAGCCTTGCAAGAAAGAGGCTTGTAACATTCAAGCTTGCCTCTCCAAGAACAACTTTCTCCCGCAAAAGTGCCTTAAAGTTATTGAATTGCTGGACTCTTGCTGCTCAAGATGCGATTATAATTCAACTCACTGTGCTTCGCTCTCTGGACTATTGAAGCAAAATTCCAAATTTCAAGACTCGGCCAAGTAA
Protein sequence

>Msa1403800

MGQESKEPCKKEACNIQACLSKNNFLPQKCLKVIELLDSCCSRCDYNSTHCASLSGLLKQNSKFQDSAK*