AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000625


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000625.t1 MTR_5g028350 97.232 289 8 0 1 289 1 289 0.0 568
MS.gene000625.t1 MTR_8g075400 76.552 290 37 4 1 289 14 273 2.26e-139 394
MS.gene000625.t1 MTR_8g075400 87.500 224 26 1 1 224 1 222 6.17e-130 369
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000625.t1 AT3G22660 65.529 293 86 6 2 290 12 293 3.65e-126 361

Find 71 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGCCTCCAAAACCAAATT+TGG 0.189093 5.1:+19136074 None:intergenic
GCTGAAACAACTAATGATTT+TGG 0.204488 5.1:-19136027 MS.gene000625:CDS
GATACTAAGAATGCCAAATT+TGG 0.209577 5.1:-19136087 MS.gene000625:CDS
AAGAATGCCAAATTTGGTTT+TGG 0.274042 5.1:-19136081 MS.gene000625:CDS
ATGGAGGTGCCTTTGATTAA+TGG 0.295822 5.1:-19136828 MS.gene000625:CDS
TCAAAGCTCAAAGACTTATC+TGG 0.326167 5.1:+19136228 None:intergenic
AGAAAGGGCCAAGCAGAAAA+AGG 0.351345 5.1:-19136322 MS.gene000625:CDS
TCAGTTTCACCGTCAATCAT+TGG 0.370634 5.1:+19136801 None:intergenic
TCTGCTTCTTCGATCCCTTC+CGG 0.371874 5.1:+19136055 None:intergenic
TGCTTCCAATCCACATTCTC+AGG 0.372391 5.1:+19136651 None:intergenic
TGACGGTGAAACTGAAGATT+TGG 0.372926 5.1:-19136793 MS.gene000625:CDS
TTCAGTAAGGGTTCTGCTGC+TGG 0.381325 5.1:-19136000 MS.gene000625:CDS
TTACCTCCTGACCGATCTCC+TGG 0.396701 5.1:+19136156 None:intergenic
AGACAATCTCTTTGCTTCCC+TGG 0.401049 5.1:+19136371 None:intergenic
AGATAAGTCTTTGAGCTTTG+AGG 0.404345 5.1:-19136226 MS.gene000625:CDS
CTGAAGAGAGAAGAAAGGCC+AGG 0.422247 5.1:-19136389 MS.gene000625:CDS
CTCTCCATTGATATTGATCA+AGG 0.429280 5.1:-19136624 MS.gene000625:CDS
TCTTCTGAATCTGATTCAAA+TGG 0.456186 5.1:+19136756 None:intergenic
AGTCACAGAAATTGAAAGAA+AGG 0.458750 5.1:-19136338 MS.gene000625:CDS
TAGTCAGCAGGTCTCAAGAA+AGG 0.478435 5.1:+19136495 None:intergenic
CTTTGAGAAGCTTGAGTCGA+TGG 0.481104 5.1:-19136523 MS.gene000625:CDS
TGCTGACTATTACGCAGAAA+TGG 0.488469 5.1:-19136481 MS.gene000625:CDS
CTCCCCTTGATCAATATCAA+TGG 0.492911 5.1:+19136620 None:intergenic
CCGATCTCCTGGAGATACTC+CGG 0.494759 5.1:+19136167 None:intergenic
AGGAGATCGGTCAGGAGGTA+AGG 0.497659 5.1:-19136154 MS.gene000625:CDS
AGTCTTTGAGCTTTGAGGAT+GGG 0.501140 5.1:-19136221 MS.gene000625:CDS
AGTCAGCAGGTCTCAAGAAA+GGG 0.501463 5.1:+19136496 None:intergenic
GGGAGATAGCCACATGGAAA+AGG 0.502899 5.1:-19136454 MS.gene000625:CDS
CATAAGCTGGCCTGAGAATG+TGG 0.503617 5.1:-19136661 MS.gene000625:CDS
GTCACAGAAATTGAAAGAAA+GGG 0.504083 5.1:-19136337 MS.gene000625:CDS
GCAAAGCAAGCTATGGGAAA+GGG 0.507585 5.1:-19136132 MS.gene000625:CDS
ATTAGATGAGAAGCGGAAGA+TGG 0.510158 5.1:-19136418 MS.gene000625:CDS
GGCATTGGAGGGAACTAGAC+AGG 0.514311 5.1:-19136547 MS.gene000625:CDS
AGGTAAGGCAAAGCAAGCTA+TGG 0.517763 5.1:-19136139 MS.gene000625:CDS
TTTGAGAAGCTTGAGTCGAT+GGG 0.525398 5.1:-19136522 MS.gene000625:CDS
GGAGGCTGAAGAGAGAAGAA+AGG 0.530598 5.1:-19136394 MS.gene000625:CDS
CAAAGACTTATCTGGAGCAT+CGG 0.536868 5.1:+19136236 None:intergenic
AGATGAGAAGCGGAAGATGG+AGG 0.539024 5.1:-19136415 MS.gene000625:CDS
GATGCTATATTAGATAAACT+TGG 0.546950 5.1:-19136687 MS.gene000625:CDS
AGCAAAGAGATTGTCTAAAG+AGG 0.557828 5.1:-19136364 MS.gene000625:CDS
AAGAGGCCCGGAGTATCTCC+AGG 0.558097 5.1:-19136174 MS.gene000625:CDS
CTCCATTGATATTGATCAAG+GGG 0.559298 5.1:-19136622 MS.gene000625:CDS
GTATCTCCAGGAGATCGGTC+AGG 0.562327 5.1:-19136162 MS.gene000625:CDS
CGATCTCCTGGAGATACTCC+GGG 0.563080 5.1:+19136168 None:intergenic
AGTAGATGTGAACGATGACT+TGG 0.565329 5.1:-19136595 MS.gene000625:CDS
ATTTCTGCGTAATAGTCAGC+AGG 0.568461 5.1:+19136483 None:intergenic
ATAAACTTGGAGACATAAGC+TGG 0.571619 5.1:-19136674 MS.gene000625:CDS
TGAAACTGAAGATTTGGATG+AGG 0.572001 5.1:-19136787 MS.gene000625:CDS
CCGGAGTATCTCCAGGAGAT+CGG 0.576336 5.1:-19136167 MS.gene000625:CDS
AAGTCTTTGAGCTTTGAGGA+TGG 0.577328 5.1:-19136222 MS.gene000625:CDS
TGAAGAGAGAAGAAAGGCCA+GGG 0.579670 5.1:-19136388 MS.gene000625:CDS
CATCGACTCAAGCTTCTCAA+AGG 0.582387 5.1:+19136524 None:intergenic
GGCAAAGCAAGCTATGGGAA+AGG 0.584114 5.1:-19136133 MS.gene000625:CDS
ATTGGATCACCATTAATCAA+AGG 0.591452 5.1:+19136819 None:intergenic
TCTCCATTGATATTGATCAA+GGG 0.594778 5.1:-19136623 MS.gene000625:CDS
AATGGTGATCCAATGATTGA+CGG 0.595326 5.1:-19136810 MS.gene000625:CDS
GGTAAGGCAAAGCAAGCTAT+GGG 0.598074 5.1:-19136138 MS.gene000625:CDS
GGAAGCAGAGACAACAGAGT+GGG 0.599709 5.1:-19136272 MS.gene000625:CDS
ACATTGAATCTGTTAAGAAG+TGG 0.600860 5.1:-19136296 MS.gene000625:CDS
TGAGAAGCGGAAGATGGAGG+AGG 0.603783 5.1:-19136412 MS.gene000625:CDS
TTGAATCTGTTAAGAAGTGG+AGG 0.608378 5.1:-19136293 MS.gene000625:CDS
CTATTACGCAGAAATGGTGA+AGG 0.608653 5.1:-19136475 MS.gene000625:CDS
GTCGGCTATTAGATGAGAAG+CGG 0.609331 5.1:-19136425 MS.gene000625:CDS
GCTGGCCTGAGAATGTGGAT+TGG 0.611875 5.1:-19136656 MS.gene000625:CDS
ACATGGAAAAGGTGAAAAGT+CGG 0.619320 5.1:-19136443 MS.gene000625:CDS
CAACAGAGTGGGTTTGCTGA+CGG 0.625190 5.1:-19136261 MS.gene000625:CDS
AGGAAGCAGAGACAACAGAG+TGG 0.651328 5.1:-19136273 MS.gene000625:CDS
CATTGATATTGATCAAGGGG+AGG 0.657330 5.1:-19136619 MS.gene000625:CDS
TCTCCAGGAGATCGGTCAGG+AGG 0.664692 5.1:-19136159 MS.gene000625:CDS
TATTACGCAGAAATGGTGAA+GGG 0.681147 5.1:-19136474 MS.gene000625:CDS
GGTGAAGGGAGATAGCCACA+TGG 0.757517 5.1:-19136460 MS.gene000625:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATGCTATATTAGATAAACT+TGG - chr5.1:19136119-19136138 MS.gene000625:CDS 25.0%
!! TTAGTATCTCTTTTCTTTTG+CGG + chr5.1:19136707-19136726 None:intergenic 25.0%
ACATTGAATCTGTTAAGAAG+TGG - chr5.1:19136510-19136529 MS.gene000625:CDS 30.0%
AGTCACAGAAATTGAAAGAA+AGG - chr5.1:19136468-19136487 MS.gene000625:CDS 30.0%
ATTGGATCACCATTAATCAA+AGG + chr5.1:19135990-19136009 None:intergenic 30.0%
GATACTAAGAATGCCAAATT+TGG - chr5.1:19136719-19136738 MS.gene000625:CDS 30.0%
GTCACAGAAATTGAAAGAAA+GGG - chr5.1:19136469-19136488 MS.gene000625:CDS 30.0%
TCTCCATTGATATTGATCAA+GGG - chr5.1:19136183-19136202 MS.gene000625:CDS 30.0%
TCTTCTGAATCTGATTCAAA+TGG + chr5.1:19136053-19136072 None:intergenic 30.0%
! GAAACAACTAATGATTTTGG+CGG - chr5.1:19136782-19136801 MS.gene000625:CDS 30.0%
! GCTGAAACAACTAATGATTT+TGG - chr5.1:19136779-19136798 MS.gene000625:CDS 30.0%
!! AAACAACTAATGATTTTGGC+GGG - chr5.1:19136783-19136802 MS.gene000625:CDS 30.0%
!! AAGAATGCCAAATTTGGTTT+TGG - chr5.1:19136725-19136744 MS.gene000625:CDS 30.0%
!!! TTATTCACTGCAGTTTTAGA+TGG + chr5.1:19136095-19136114 None:intergenic 30.0%
AATGGTGATCCAATGATTGA+CGG - chr5.1:19135996-19136015 MS.gene000625:CDS 35.0%
ACATGGAAAAGGTGAAAAGT+CGG - chr5.1:19136363-19136382 MS.gene000625:CDS 35.0%
AGATAAGTCTTTGAGCTTTG+AGG - chr5.1:19136580-19136599 MS.gene000625:CDS 35.0%
AGCAAAGAGATTGTCTAAAG+AGG - chr5.1:19136442-19136461 MS.gene000625:CDS 35.0%
ATAAACTTGGAGACATAAGC+TGG - chr5.1:19136132-19136151 MS.gene000625:CDS 35.0%
CTCCATTGATATTGATCAAG+GGG - chr5.1:19136184-19136203 MS.gene000625:CDS 35.0%
CTCTCCATTGATATTGATCA+AGG - chr5.1:19136182-19136201 MS.gene000625:CDS 35.0%
TATTACGCAGAAATGGTGAA+GGG - chr5.1:19136332-19136351 MS.gene000625:CDS 35.0%
TCAAAGCTCAAAGACTTATC+TGG + chr5.1:19136581-19136600 None:intergenic 35.0%
TCGAGAGATCAAAGAAAAAG+AGG - chr5.1:19136615-19136634 MS.gene000625:CDS 35.0%
TGAAACTGAAGATTTGGATG+AGG - chr5.1:19136019-19136038 MS.gene000625:CDS 35.0%
TTGAATCTGTTAAGAAGTGG+AGG - chr5.1:19136513-19136532 MS.gene000625:CDS 35.0%
! AATGTCATCCTTTTTCTGCT+TGG + chr5.1:19136495-19136514 None:intergenic 35.0%
!!! AATGCCAAATTTGGTTTTGG+AGG - chr5.1:19136728-19136747 MS.gene000625:CDS 35.0%
AGATCAAAGAAAAAGAGGCC+CGG - chr5.1:19136620-19136639 MS.gene000625:CDS 40.0%
AGTAGATGTGAACGATGACT+TGG - chr5.1:19136211-19136230 MS.gene000625:CDS 40.0%
ATTAGATGAGAAGCGGAAGA+TGG - chr5.1:19136388-19136407 MS.gene000625:CDS 40.0%
ATTTCTGCGTAATAGTCAGC+AGG + chr5.1:19136326-19136345 None:intergenic 40.0%
CATTGATATTGATCAAGGGG+AGG - chr5.1:19136187-19136206 MS.gene000625:CDS 40.0%
CTATTACGCAGAAATGGTGA+AGG - chr5.1:19136331-19136350 MS.gene000625:CDS 40.0%
CTCCCCTTGATCAATATCAA+TGG + chr5.1:19136189-19136208 None:intergenic 40.0%
TCAGTTTCACCGTCAATCAT+TGG + chr5.1:19136008-19136027 None:intergenic 40.0%
TGACGGTGAAACTGAAGATT+TGG - chr5.1:19136013-19136032 MS.gene000625:CDS 40.0%
TTTGAGAAGCTTGAGTCGAT+GGG - chr5.1:19136284-19136303 MS.gene000625:CDS 40.0%
! ATCATTTTACACGCAGGCAT+TGG - chr5.1:19136244-19136263 MS.gene000625:CDS 40.0%
! CAAAGACTTATCTGGAGCAT+CGG + chr5.1:19136573-19136592 None:intergenic 40.0%
! GACTTTTCACCTTTTCCATG+TGG + chr5.1:19136364-19136383 None:intergenic 40.0%
! TGAGCTATCATTTTACACGC+AGG - chr5.1:19136238-19136257 MS.gene000625:CDS 40.0%
! TGCTGACTATTACGCAGAAA+TGG - chr5.1:19136325-19136344 MS.gene000625:CDS 40.0%
!! AAGTCTTTGAGCTTTGAGGA+TGG - chr5.1:19136584-19136603 MS.gene000625:CDS 40.0%
!! AGTCTTTGAGCTTTGAGGAT+GGG - chr5.1:19136585-19136604 MS.gene000625:CDS 40.0%
!! ATGGAGGTGCCTTTGATTAA+TGG - chr5.1:19135978-19135997 MS.gene000625:CDS 40.0%
AGAAAGGGCCAAGCAGAAAA+AGG - chr5.1:19136484-19136503 MS.gene000625:CDS 45.0%
AGACAATCTCTTTGCTTCCC+TGG + chr5.1:19136438-19136457 None:intergenic 45.0%
AGGTAAGGCAAAGCAAGCTA+TGG - chr5.1:19136667-19136686 MS.gene000625:CDS 45.0%
AGTCAGCAGGTCTCAAGAAA+GGG + chr5.1:19136313-19136332 None:intergenic 45.0%
GCAAAGCAAGCTATGGGAAA+GGG - chr5.1:19136674-19136693 MS.gene000625:CDS 45.0%
GGTAAGGCAAAGCAAGCTAT+GGG - chr5.1:19136668-19136687 MS.gene000625:CDS 45.0%
GTCGGCTATTAGATGAGAAG+CGG - chr5.1:19136381-19136400 MS.gene000625:CDS 45.0%
TAGTCAGCAGGTCTCAAGAA+AGG + chr5.1:19136314-19136333 None:intergenic 45.0%
TGAAGAGAGAAGAAAGGCCA+GGG - chr5.1:19136418-19136437 MS.gene000625:CDS 45.0%
TGCTTCCAATCCACATTCTC+AGG + chr5.1:19136158-19136177 None:intergenic 45.0%
! CTTTGAGAAGCTTGAGTCGA+TGG - chr5.1:19136283-19136302 MS.gene000625:CDS 45.0%
!! CATCGACTCAAGCTTCTCAA+AGG + chr5.1:19136285-19136304 None:intergenic 45.0%
!!! ATTTTACACGCAGGCATTGG+AGG - chr5.1:19136247-19136266 MS.gene000625:CDS 45.0%
!!! CCAAATTTGGTTTTGGAGGC+CGG - chr5.1:19136732-19136751 MS.gene000625:CDS 45.0%
!!! GATTTTGGCGGGTTCAGTAA+GGG - chr5.1:19136794-19136813 MS.gene000625:CDS 45.0%
!!! TGATTTTGGCGGGTTCAGTA+AGG - chr5.1:19136793-19136812 MS.gene000625:CDS 45.0%
!!! TTTTACACGCAGGCATTGGA+GGG - chr5.1:19136248-19136267 MS.gene000625:CDS 45.0%
AGATGAGAAGCGGAAGATGG+AGG - chr5.1:19136391-19136410 MS.gene000625:CDS 50.0%
AGGAAGCAGAGACAACAGAG+TGG - chr5.1:19136533-19136552 MS.gene000625:CDS 50.0%
CCGGCCTCCAAAACCAAATT+TGG + chr5.1:19136735-19136754 None:intergenic 50.0%
CTGAAGAGAGAAGAAAGGCC+AGG - chr5.1:19136417-19136436 MS.gene000625:CDS 50.0%
GGAAGCAGAGACAACAGAGT+GGG - chr5.1:19136534-19136553 MS.gene000625:CDS 50.0%
GGAGGCTGAAGAGAGAAGAA+AGG - chr5.1:19136412-19136431 MS.gene000625:CDS 50.0%
GGCAAAGCAAGCTATGGGAA+AGG - chr5.1:19136673-19136692 MS.gene000625:CDS 50.0%
GGGAGATAGCCACATGGAAA+AGG - chr5.1:19136352-19136371 MS.gene000625:CDS 50.0%
TCTGCTTCTTCGATCCCTTC+CGG + chr5.1:19136754-19136773 None:intergenic 50.0%
TTCAGTAAGGGTTCTGCTGC+TGG - chr5.1:19136806-19136825 MS.gene000625:CDS 50.0%
! CATAAGCTGGCCTGAGAATG+TGG - chr5.1:19136145-19136164 MS.gene000625:CDS 50.0%
!! CAACAGAGTGGGTTTGCTGA+CGG - chr5.1:19136545-19136564 MS.gene000625:CDS 50.0%
!!! ATTTGGTTTTGGAGGCCGGA+AGG - chr5.1:19136736-19136755 MS.gene000625:CDS 50.0%
!!! TTTGGTTTTGGAGGCCGGAA+GGG - chr5.1:19136737-19136756 MS.gene000625:CDS 50.0%
AGGAGATCGGTCAGGAGGTA+AGG - chr5.1:19136652-19136671 MS.gene000625:CDS 55.0%
CCGATCTCCTGGAGATACTC+CGG + chr5.1:19136642-19136661 None:intergenic 55.0%
CGATCTCCTGGAGATACTCC+GGG + chr5.1:19136641-19136660 None:intergenic 55.0%
GGTGAAGGGAGATAGCCACA+TGG - chr5.1:19136346-19136365 MS.gene000625:CDS 55.0%
GTATCTCCAGGAGATCGGTC+AGG - chr5.1:19136644-19136663 MS.gene000625:CDS 55.0%
TGAGAAGCGGAAGATGGAGG+AGG - chr5.1:19136394-19136413 MS.gene000625:CDS 55.0%
TTACCTCCTGACCGATCTCC+TGG + chr5.1:19136653-19136672 None:intergenic 55.0%
! CCGGAGTATCTCCAGGAGAT+CGG - chr5.1:19136639-19136658 MS.gene000625:CDS 55.0%
! GCTGGCCTGAGAATGTGGAT+TGG - chr5.1:19136150-19136169 MS.gene000625:CDS 55.0%
! GGCATTGGAGGGAACTAGAC+AGG - chr5.1:19136259-19136278 MS.gene000625:CDS 55.0%
TCTCCAGGAGATCGGTCAGG+AGG - chr5.1:19136647-19136666 MS.gene000625:CDS 60.0%
! AAGAGGCCCGGAGTATCTCC+AGG - chr5.1:19136632-19136651 MS.gene000625:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 19135978 19136850 19135978 ID=MS.gene000625
chr5.1 mRNA 19135978 19136850 19135978 ID=MS.gene000625.t1;Parent=MS.gene000625
chr5.1 exon 19135978 19136850 19135978 ID=MS.gene000625.t1.exon1;Parent=MS.gene000625.t1
chr5.1 CDS 19135978 19136850 19135978 ID=cds.MS.gene000625.t1;Parent=MS.gene000625.t1
Gene Sequence

>MS.gene000625

ATGGAGGTGCCTTTGATTAATGGTGATCCAATGATTGACGGTGAAACTGAAGATTTGGATGAGGAAATGTCACCATTTGAATCAGATTCAGAAGAAGATGTCAAATTAGCTGAACCATCTAAAACTGCAGTGAATAACAGAGATGCTATATTAGATAAACTTGGAGACATAAGCTGGCCTGAGAATGTGGATTGGAAGCACAAACTCTCCATTGATATTGATCAAGGGGAGGAAGTAGATGTGAACGATGACTTGGCACGTGAGCTATCATTTTACACGCAGGCATTGGAGGGAACTAGACAGGCCTTTGAGAAGCTTGAGTCGATGGGTCTCCCTTTCTTGAGACCTGCTGACTATTACGCAGAAATGGTGAAGGGAGATAGCCACATGGAAAAGGTGAAAAGTCGGCTATTAGATGAGAAGCGGAAGATGGAGGAGGCTGAAGAGAGAAGAAAGGCCAGGGAAGCAAAGAGATTGTCTAAAGAGGTTCAGTCACAGAAATTGAAAGAAAGGGCCAAGCAGAAAAAGGATGACATTGAATCTGTTAAGAAGTGGAGGAAGCAGAGACAACAGAGTGGGTTTGCTGACGGCGCCGATGCTCCAGATAAGTCTTTGAGCTTTGAGGATGGGAAAGTGTTCGAGAGATCAAAGAAAAAGAGGCCCGGAGTATCTCCAGGAGATCGGTCAGGAGGTAAGGCAAAGCAAGCTATGGGAAAGGGAAAGAAGCCGCAAAAGAAAAGAGATACTAAGAATGCCAAATTTGGTTTTGGAGGCCGGAAGGGATCGAAGAAGCAGAATACAGCTGAAACAACTAATGATTTTGGCGGGTTCAGTAAGGGTTCTGCTGCTGGAAATAAGAAGAGAAAGAGATAA

Protein sequence

>MS.gene000625.t1

MEVPLINGDPMIDGETEDLDEEMSPFESDSEEDVKLAEPSKTAVNNRDAILDKLGDISWPENVDWKHKLSIDIDQGEEVDVNDDLARELSFYTQALEGTRQAFEKLESMGLPFLRPADYYAEMVKGDSHMEKVKSRLLDEKRKMEEAEERRKAREAKRLSKEVQSQKLKERAKQKKDDIESVKKWRKQRQQSGFADGADAPDKSLSFEDGKVFERSKKKRPGVSPGDRSGGKAKQAMGKGKKPQKKRDTKNAKFGFGGRKGSKKQNTAETTNDFGGFSKGSAAGNKKRKR