AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene039132

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039132.t1 MTR_2g064460 87.472 447 35 2 1 446 1 427 0.0 669
MS.gene039132.t1 MTR_2g064460 87.500 440 34 2 1 439 1 420 0.0 661
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039132.t1 AT3G58660 50.799 313 135 4 10 306 28 337 1.75e-106 323
MS.gene039132.t1 AT2G42650 50.313 320 139 8 10 315 8 321 4.19e-101 306
MS.gene039132.t1 AT1G06380 50.385 260 106 5 1 244 1 253 8.71e-81 250

Find 91 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGCTTTGCCGGTTTATC+AGG 0.252561 2.2:-22727729 MS.gene039132:CDS
GTTGGAGTCTCTTGCTTTGC+CGG 0.274544 2.2:-22727738 MS.gene039132:CDS
TTCGTCTTCGTCGAAGAGTT+TGG 0.278193 2.2:+22728342 None:intergenic
GCCGTTGTTGCCGAGGTTAT+TGG 0.297689 2.2:-22728014 MS.gene039132:CDS
TGCGACGGTGTCGGCGTTGC+TGG 0.306658 2.2:+22728420 None:intergenic
CCGTTGTTGCCGAGGTTATT+GGG 0.308899 2.2:-22728013 MS.gene039132:CDS
TTTCTTCTGAGCTTCGTCTT+TGG 0.323418 2.2:+22728168 None:intergenic
TCGTCTTCGTCGAAGAGTTT+GGG 0.362865 2.2:+22728343 None:intergenic
ATCAGGCTGTTCCTGATGTT+AGG 0.368452 2.2:-22727712 MS.gene039132:CDS
AGCTTAAGAATCTTCGAAAT+AGG 0.370256 2.2:+22728127 None:intergenic
GATTCATGAGATTAAGTATA+TGG 0.374104 2.2:-22727597 MS.gene039132:CDS
TAAGGATTTGGAGGATAAGT+TGG 0.380856 2.2:-22727672 MS.gene039132:CDS
GAGATGAGAGAGTGAGGGAT+TGG 0.381358 2.2:+22728250 None:intergenic
TGGATTTGCAGAAGAAGAAT+TGG 0.381611 2.2:-22727952 MS.gene039132:CDS
AGATGAGAGAGTGAGGGATT+GGG 0.425499 2.2:+22728251 None:intergenic
GAAGGTGGCTAAGTTGAGTA+TGG 0.428280 2.2:-22727861 MS.gene039132:CDS
GAAGTTATCCGTGAAGGAAA+AGG 0.432264 2.2:-22727279 MS.gene039132:CDS
AAAAGAGGATTCACGTGAGT+TGG 0.433236 2.2:-22727405 MS.gene039132:CDS
GTTTAGCGGAAATGAATCAT+TGG 0.447165 2.2:-22727183 MS.gene039132:CDS
ATCGTCCGTTTGAGGCGAAA+AGG 0.448339 2.2:-22728084 MS.gene039132:CDS
TCAGGAACAGCCTGATAAAC+CGG 0.448391 2.2:+22727719 None:intergenic
TGAATCATTGGTCAAGGATG+AGG 0.453041 2.2:-22727171 MS.gene039132:CDS
TGATGTTAGGTTGAAGATTG+AGG 0.457382 2.2:-22727699 MS.gene039132:CDS
GTGAGTAAGAAGAGGAAGAA+AGG 0.462622 2.2:-22727434 MS.gene039132:CDS
GTTGATGATGATAGTGAGAT+TGG 0.469449 2.2:-22727479 MS.gene039132:CDS
AAAGAGGATTCACGTGAGTT+GGG 0.472135 2.2:-22727404 MS.gene039132:CDS
CCGTAGACTCTCTTCTCAAA+TGG 0.472791 2.2:-22728393 MS.gene039132:CDS
CAAAGAAGAAGAATAACAAA+AGG 0.474258 2.2:-22727253 MS.gene039132:CDS
GAAGAGGAAGATTCCGGTGC+AGG 0.476004 2.2:-22727975 MS.gene039132:CDS
GAAGAAGTGCTCATGTTTAG+CGG 0.478710 2.2:-22727197 MS.gene039132:CDS
AGAAGAGTAAGAAGAAAGGT+AGG 0.479352 2.2:-22727619 MS.gene039132:CDS
AAAGAAGAAGAATAACAAAA+GGG 0.480788 2.2:-22727252 MS.gene039132:CDS
ATTGGTCAAGGATGAGGGTT+CGG 0.485154 2.2:-22727165 MS.gene039132:CDS
GATGTTAGGTTGAAGATTGA+GGG 0.486312 2.2:-22727698 MS.gene039132:CDS
CTCTCTCATCTCCGCAGCAT+CGG 0.488711 2.2:-22728239 MS.gene039132:CDS
GCTAAGAGGAAGAAGAAAGA+TGG 0.489873 2.2:-22727356 MS.gene039132:CDS
AGCTCAGAAGAAAGTTCAAT+CGG 0.490415 2.2:-22728158 MS.gene039132:CDS
CTAAAGAAAATTCCATCAAA+AGG 0.498789 2.2:-22728295 MS.gene039132:CDS
TTCTGCAAATCCACCTGCAC+CGG 0.501939 2.2:+22727962 None:intergenic
GGGAAGTGTGAAATCATTGA+AGG 0.502129 2.2:-22727384 MS.gene039132:CDS
AAGTTATCCGTGAAGGAAAA+GGG 0.506296 2.2:-22727278 MS.gene039132:CDS
CCCAATAACCTCGGCAACAA+CGG 0.513459 2.2:+22728013 None:intergenic
GGTGTCGGCGTTGCTGGTTA+CGG 0.518609 2.2:+22728426 None:intergenic
TGAGAATGTTGTTGCTGCGA+TGG 0.520350 2.2:-22727822 MS.gene039132:CDS
GACGGATTATCGTCCGTTTG+AGG 0.522523 2.2:-22728092 MS.gene039132:CDS
TTTGGAGGATAAGTTGGTGA+TGG 0.530137 2.2:-22727666 MS.gene039132:CDS
TGGGACTTGTAGTGTGGTGA+AGG 0.531069 2.2:-22727879 MS.gene039132:CDS
CAAGAGTAAGAAAAGTAAGA+AGG 0.531808 2.2:-22727108 MS.gene039132:CDS
AAGAAGAAGAATAACAAAAG+GGG 0.535687 2.2:-22727251 MS.gene039132:CDS
CATTGAAGGGTTCTGCTAAG+AGG 0.537258 2.2:-22727370 MS.gene039132:CDS
GAGATTGGTGATATCGAGAG+TGG 0.537914 2.2:-22727464 MS.gene039132:CDS
GTTGGTGATGGATGAGAAGA+AGG 0.538960 2.2:-22727654 MS.gene039132:CDS
TCAATCTTCAACCTAACATC+AGG 0.541413 2.2:+22727701 None:intergenic
TGTTAGTGGTGGTGGTGATG+AGG 0.543748 2.2:-22727501 MS.gene039132:CDS
CCATTTGAGAAGAGAGTCTA+CGG 0.545460 2.2:+22728393 None:intergenic
GAGGGTTGGTGAGTAAGAAG+AGG 0.551935 2.2:-22727442 MS.gene039132:CDS
GCGAAGAAGAGTAAGAAGAA+AGG 0.559141 2.2:-22727623 MS.gene039132:CDS
TCGTCTTTGGTGATACGAGA+GGG 0.562374 2.2:+22728181 None:intergenic
AATGTGAATGCTGCAGAAGA+AGG 0.562909 2.2:-22727329 MS.gene039132:CDS
CGGAAATGAATCATTGGTCA+AGG 0.564280 2.2:-22727177 MS.gene039132:CDS
CGTTGTTGCCGAGGTTATTG+GGG 0.570182 2.2:-22728012 MS.gene039132:CDS
GAGGAAGATTCCGGTGCAGG+TGG 0.571715 2.2:-22727972 MS.gene039132:CDS
TTGGTGATATCGAGAGTGGA+GGG 0.571912 2.2:-22727460 MS.gene039132:CDS
TGTTAGGTTGAAGATTGAGG+GGG 0.574768 2.2:-22727696 MS.gene039132:CDS
CTATTGTGCCGTTGTTGCCG+AGG 0.574937 2.2:-22728021 MS.gene039132:CDS
TTCGTCTTTGGTGATACGAG+AGG 0.580043 2.2:+22728180 None:intergenic
GAATAAGAAGTTATCCGTGA+AGG 0.580061 2.2:-22727285 MS.gene039132:CDS
CTAAGAGGAAGAAGAAAGAT+GGG 0.580491 2.2:-22727355 MS.gene039132:CDS
GAGAAAGACGGATGATGTGA+AGG 0.580519 2.2:-22727132 MS.gene039132:CDS
GACTTGTAGTGTGGTGAAGG+TGG 0.580675 2.2:-22727876 MS.gene039132:CDS
GAATCATTGGTCAAGGATGA+GGG 0.583068 2.2:-22727170 MS.gene039132:CDS
GAATGTTGTTGCTGCGATGG+AGG 0.588380 2.2:-22727819 MS.gene039132:CDS
ATTGGTGATATCGAGAGTGG+AGG 0.594236 2.2:-22727461 MS.gene039132:CDS
AACTGCTTCCCCAATAACCT+CGG 0.596591 2.2:+22728004 None:intergenic
TTGGAAGGAGCAGATTGAGA+AGG 0.597099 2.2:-22727933 MS.gene039132:CDS
AATGTTGTTGCTGCGATGGA+GGG 0.600510 2.2:-22727818 MS.gene039132:CDS
AGAAGAAGAATAACAAAAGG+GGG 0.601080 2.2:-22727250 MS.gene039132:CDS
GATGATAGAATGAGTTCAGA+TGG 0.609600 2.2:-22727575 MS.gene039132:CDS
ATGTTAGGTTGAAGATTGAG+GGG 0.617593 2.2:-22727697 MS.gene039132:CDS
TGCGATGGAGGGTGTTGTCG+AGG 0.617796 2.2:-22727807 MS.gene039132:CDS
GCTGCGGAGATGAGAGAGTG+AGG 0.618794 2.2:+22728244 None:intergenic
GAGGACTGGGACTTGTAGTG+TGG 0.619046 2.2:-22727885 MS.gene039132:CDS
GGAAGTGTGAAATCATTGAA+GGG 0.619801 2.2:-22727383 MS.gene039132:CDS
TGATATCGAGAGTGGAGGGT+TGG 0.619964 2.2:-22727456 MS.gene039132:CDS
TTTGCAGAAGAAGAATTGGA+AGG 0.627135 2.2:-22727948 MS.gene039132:CDS
TAAGCTTTCGAAGCTCATGA+CGG 0.630267 2.2:-22728110 MS.gene039132:CDS
GTCGGCGTTGCTGGTTACGG+TGG 0.647021 2.2:+22728429 None:intergenic
GAGACACTGTTCCGATGCTG+CGG 0.652900 2.2:+22728228 None:intergenic
CTGCGGAGATGAGAGAGTGA+GGG 0.658572 2.2:+22728245 None:intergenic
GGACAAGAAGAAGAGAAAGA+CGG 0.680373 2.2:-22727144 MS.gene039132:CDS
GAAGAAGAATAACAAAAGGG+GGG 0.703279 2.2:-22727249 MS.gene039132:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAGAAGAATAACAAAA+GGG - chr2.2:22728281-22728300 MS.gene039132:CDS 20.0%
!!! TTTTGATGGAATTTTCTTTA+GGG + chr2.2:22727239-22727258 None:intergenic 20.0%
! AAGAAGAAGAATAACAAAAG+GGG - chr2.2:22728282-22728301 MS.gene039132:CDS 25.0%
! CAAAGAAGAAGAATAACAAA+AGG - chr2.2:22728280-22728299 MS.gene039132:CDS 25.0%
! CTAAAGAAAATTCCATCAAA+AGG - chr2.2:22727238-22727257 MS.gene039132:CDS 25.0%
! GATTCATGAGATTAAGTATA+TGG - chr2.2:22727936-22727955 MS.gene039132:CDS 25.0%
!! CTTTTGATGGAATTTTCTTT+AGG + chr2.2:22727240-22727259 None:intergenic 25.0%
AGAAGAAGAATAACAAAAGG+GGG - chr2.2:22728283-22728302 MS.gene039132:CDS 30.0%
AGCTTAAGAATCTTCGAAAT+AGG + chr2.2:22727409-22727428 None:intergenic 30.0%
CAAGAGTAAGAAAAGTAAGA+AGG - chr2.2:22728425-22728444 MS.gene039132:CDS 30.0%
! ATCGATGATGATTTTGTTAG+TGG - chr2.2:22728018-22728037 MS.gene039132:CDS 30.0%
!! GTCTTTTCATGTTAAGTTGT+TGG - chr2.2:22727777-22727796 MS.gene039132:CDS 30.0%
!! TTTTAAGAAGAGGAAGATTC+CGG - chr2.2:22727552-22727571 MS.gene039132:CDS 30.0%
!!! ATGGAATTTTCTTTAGGGTT+AGG + chr2.2:22727234-22727253 None:intergenic 30.0%
AAGTTATCCGTGAAGGAAAA+GGG - chr2.2:22728255-22728274 MS.gene039132:CDS 35.0%
AGAAGAGTAAGAAGAAAGGT+AGG - chr2.2:22727914-22727933 MS.gene039132:CDS 35.0%
AGCTCAGAAGAAAGTTCAAT+CGG - chr2.2:22727375-22727394 MS.gene039132:CDS 35.0%
CTAAGAGGAAGAAGAAAGAT+GGG - chr2.2:22728178-22728197 MS.gene039132:CDS 35.0%
GAAGAAAGGAGTGAAAAAAG+AGG - chr2.2:22728113-22728132 MS.gene039132:CDS 35.0%
GAAGAAGAATAACAAAAGGG+GGG - chr2.2:22728284-22728303 MS.gene039132:CDS 35.0%
GAATAAGAAGTTATCCGTGA+AGG - chr2.2:22728248-22728267 MS.gene039132:CDS 35.0%
GATGATAGAATGAGTTCAGA+TGG - chr2.2:22727958-22727977 MS.gene039132:CDS 35.0%
GTTTAGCGGAAATGAATCAT+TGG - chr2.2:22728350-22728369 MS.gene039132:CDS 35.0%
TAAGGATTTGGAGGATAAGT+TGG - chr2.2:22727861-22727880 MS.gene039132:CDS 35.0%
TCAATCTTCAACCTAACATC+AGG + chr2.2:22727835-22727854 None:intergenic 35.0%
TGGATTTGCAGAAGAAGAAT+TGG - chr2.2:22727581-22727600 MS.gene039132:CDS 35.0%
TTTGCAGAAGAAGAATTGGA+AGG - chr2.2:22727585-22727604 MS.gene039132:CDS 35.0%
! GATGATGATTTTGTTAGTGG+TGG - chr2.2:22728021-22728040 MS.gene039132:CDS 35.0%
! GGAAGTGTGAAATCATTGAA+GGG - chr2.2:22728150-22728169 MS.gene039132:CDS 35.0%
!! ATGTTAGGTTGAAGATTGAG+GGG - chr2.2:22727836-22727855 MS.gene039132:CDS 35.0%
!! GATGTTAGGTTGAAGATTGA+GGG - chr2.2:22727835-22727854 MS.gene039132:CDS 35.0%
!! GTTGATGATGATAGTGAGAT+TGG - chr2.2:22728054-22728073 MS.gene039132:CDS 35.0%
!! TGATGTTAGGTTGAAGATTG+AGG - chr2.2:22727834-22727853 MS.gene039132:CDS 35.0%
!!! CTTCTGCTTTGTTGTTTTTG+AGG - chr2.2:22727629-22727648 MS.gene039132:CDS 35.0%
!!! CTTTGTTGTTTTTGAGGACT+GGG - chr2.2:22727635-22727654 MS.gene039132:CDS 35.0%
!!! GGAAGCAGTTTTTTAAGAAG+AGG - chr2.2:22727542-22727561 MS.gene039132:CDS 35.0%
!!! TTTTCTTTAGGGTTAGGATG+AGG + chr2.2:22727228-22727247 None:intergenic 35.0%
AAAAGAGGATTCACGTGAGT+TGG - chr2.2:22728128-22728147 MS.gene039132:CDS 40.0%
AAAGAGGATTCACGTGAGTT+GGG - chr2.2:22728129-22728148 MS.gene039132:CDS 40.0%
AATGTGAATGCTGCAGAAGA+AGG - chr2.2:22728204-22728223 MS.gene039132:CDS 40.0%
CCATTTGAGAAGAGAGTCTA+CGG + chr2.2:22727143-22727162 None:intergenic 40.0%
CGGAAATGAATCATTGGTCA+AGG - chr2.2:22728356-22728375 MS.gene039132:CDS 40.0%
GAAGTTATCCGTGAAGGAAA+AGG - chr2.2:22728254-22728273 MS.gene039132:CDS 40.0%
GAATCATTGGTCAAGGATGA+GGG - chr2.2:22728363-22728382 MS.gene039132:CDS 40.0%
GCGAAGAAGAGTAAGAAGAA+AGG - chr2.2:22727910-22727929 MS.gene039132:CDS 40.0%
GCTAAGAGGAAGAAGAAAGA+TGG - chr2.2:22728177-22728196 MS.gene039132:CDS 40.0%
GGACAAGAAGAAGAGAAAGA+CGG - chr2.2:22728389-22728408 MS.gene039132:CDS 40.0%
GTGAGTAAGAAGAGGAAGAA+AGG - chr2.2:22728099-22728118 MS.gene039132:CDS 40.0%
TAAGCTTTCGAAGCTCATGA+CGG - chr2.2:22727423-22727442 MS.gene039132:CDS 40.0%
TGAATCATTGGTCAAGGATG+AGG - chr2.2:22728362-22728381 MS.gene039132:CDS 40.0%
TTTCTTCTGAGCTTCGTCTT+TGG + chr2.2:22727368-22727387 None:intergenic 40.0%
! AACTTCCTTTTCGCCTCAAA+CGG + chr2.2:22727457-22727476 None:intergenic 40.0%
! AGGTTTTACCGAAGAAATGG+GGG - chr2.2:22727746-22727765 MS.gene039132:CDS 40.0%
! CGAGGTTTTACCGAAGAAAT+GGG - chr2.2:22727744-22727763 MS.gene039132:CDS 40.0%
! CTTTGAACCCTTTTCCTTCA+CGG + chr2.2:22728265-22728284 None:intergenic 40.0%
! GAGGTTTTACCGAAGAAATG+GGG - chr2.2:22727745-22727764 MS.gene039132:CDS 40.0%
! GATGATTTTGTTAGTGGTGG+TGG - chr2.2:22728024-22728043 MS.gene039132:CDS 40.0%
! GGGAAGTGTGAAATCATTGA+AGG - chr2.2:22728149-22728168 MS.gene039132:CDS 40.0%
! TCGAGGTTTTACCGAAGAAA+TGG - chr2.2:22727743-22727762 MS.gene039132:CDS 40.0%
!! GAAGAAGTGCTCATGTTTAG+CGG - chr2.2:22728336-22728355 MS.gene039132:CDS 40.0%
!! TGTTAGGTTGAAGATTGAGG+GGG - chr2.2:22727837-22727856 MS.gene039132:CDS 40.0%
!! TTTGGAGGATAAGTTGGTGA+TGG - chr2.2:22727867-22727886 MS.gene039132:CDS 40.0%
!!! GCTTTGTTGTTTTTGAGGAC+TGG - chr2.2:22727634-22727653 MS.gene039132:CDS 40.0%
AACTGCTTCCCCAATAACCT+CGG + chr2.2:22727532-22727551 None:intergenic 45.0%
AATGTTGTTGCTGCGATGGA+GGG - chr2.2:22727715-22727734 MS.gene039132:CDS 45.0%
AGATGAGAGAGTGAGGGATT+GGG + chr2.2:22727285-22727304 None:intergenic 45.0%
ATCAGGCTGTTCCTGATGTT+AGG - chr2.2:22727821-22727840 MS.gene039132:CDS 45.0%
ATTGGTCAAGGATGAGGGTT+CGG - chr2.2:22728368-22728387 MS.gene039132:CDS 45.0%
CATTGAAGGGTTCTGCTAAG+AGG - chr2.2:22728163-22728182 MS.gene039132:CDS 45.0%
CCGTAGACTCTCTTCTCAAA+TGG - chr2.2:22727140-22727159 MS.gene039132:CDS 45.0%
CTAACAACCCCCCATTTCTT+CGG + chr2.2:22727757-22727776 None:intergenic 45.0%
GAAGAATAACAAAAGGGGGG+TGG - chr2.2:22728287-22728306 MS.gene039132:CDS 45.0%
GAGAAAGACGGATGATGTGA+AGG - chr2.2:22728401-22728420 MS.gene039132:CDS 45.0%
GATTGAGGGGGTTAAGGATT+TGG - chr2.2:22727849-22727868 MS.gene039132:CDS 45.0%
GTTGAAGATTGAGGGGGTTA+AGG - chr2.2:22727843-22727862 MS.gene039132:CDS 45.0%
TCAGGAACAGCCTGATAAAC+CGG + chr2.2:22727817-22727836 None:intergenic 45.0%
TCGTCTTCGTCGAAGAGTTT+GGG + chr2.2:22727193-22727212 None:intergenic 45.0%
TGAGAATGTTGTTGCTGCGA+TGG - chr2.2:22727711-22727730 MS.gene039132:CDS 45.0%
TTCGTCTTCGTCGAAGAGTT+TGG + chr2.2:22727194-22727213 None:intergenic 45.0%
TTGGAAGGAGCAGATTGAGA+AGG - chr2.2:22727600-22727619 MS.gene039132:CDS 45.0%
! ATTGGTGATATCGAGAGTGG+AGG - chr2.2:22728072-22728091 MS.gene039132:CDS 45.0%
! GAAGGTGGCTAAGTTGAGTA+TGG - chr2.2:22727672-22727691 MS.gene039132:CDS 45.0%
! GAGATTGGTGATATCGAGAG+TGG - chr2.2:22728069-22728088 MS.gene039132:CDS 45.0%
! GAGGGATTGGGATTTTGTGA+GGG + chr2.2:22727273-22727292 None:intergenic 45.0%
! GGTTTTACCGAAGAAATGGG+GGG - chr2.2:22727747-22727766 MS.gene039132:CDS 45.0%
! TCGTCTTTGGTGATACGAGA+GGG + chr2.2:22727355-22727374 None:intergenic 45.0%
! TGAGGGATTGGGATTTTGTG+AGG + chr2.2:22727274-22727293 None:intergenic 45.0%
! TTCGTCTTTGGTGATACGAG+AGG + chr2.2:22727356-22727375 None:intergenic 45.0%
! TTGGTGATATCGAGAGTGGA+GGG - chr2.2:22728073-22728092 MS.gene039132:CDS 45.0%
!! GTTGGTGATGGATGAGAAGA+AGG - chr2.2:22727879-22727898 MS.gene039132:CDS 45.0%
!! TCTTGCTTTGCCGGTTTATC+AGG - chr2.2:22727804-22727823 MS.gene039132:CDS 45.0%
CCCAATAACCTCGGCAACAA+CGG + chr2.2:22727523-22727542 None:intergenic 50.0%
CCGTTGTTGCCGAGGTTATT+GGG - chr2.2:22727520-22727539 MS.gene039132:CDS 50.0%
CGAAGAAATGGGGGGTTGTT+AGG - chr2.2:22727755-22727774 MS.gene039132:CDS 50.0%
CGTTGTTGCCGAGGTTATTG+GGG - chr2.2:22727521-22727540 MS.gene039132:CDS 50.0%
GAATGTTGTTGCTGCGATGG+AGG - chr2.2:22727714-22727733 MS.gene039132:CDS 50.0%
GACTTGTAGTGTGGTGAAGG+TGG - chr2.2:22727657-22727676 MS.gene039132:CDS 50.0%
GAGATGAGAGAGTGAGGGAT+TGG + chr2.2:22727286-22727305 None:intergenic 50.0%
GTTGGAGTCTCTTGCTTTGC+CGG - chr2.2:22727795-22727814 MS.gene039132:CDS 50.0%
TGAGGGGGTTAAGGATTTGG+AGG - chr2.2:22727852-22727871 MS.gene039132:CDS 50.0%
TGATATCGAGAGTGGAGGGT+TGG - chr2.2:22728077-22728096 MS.gene039132:CDS 50.0%
TGGGACTTGTAGTGTGGTGA+AGG - chr2.2:22727654-22727673 MS.gene039132:CDS 50.0%
TTCTGCAAATCCACCTGCAC+CGG + chr2.2:22727574-22727593 None:intergenic 50.0%
! AGAGTCTACGGCTTTTGCGA+CGG + chr2.2:22727131-22727150 None:intergenic 50.0%
! ATCGTCCGTTTGAGGCGAAA+AGG - chr2.2:22727449-22727468 MS.gene039132:CDS 50.0%
! GGGTTAACGCGACCTTTTGA+TGG + chr2.2:22727253-22727272 None:intergenic 50.0%
!! GACGGATTATCGTCCGTTTG+AGG - chr2.2:22727441-22727460 MS.gene039132:CDS 50.0%
!! GAGGGTTGGTGAGTAAGAAG+AGG - chr2.2:22728091-22728110 MS.gene039132:CDS 50.0%
!! TGTTAGTGGTGGTGGTGATG+AGG - chr2.2:22728032-22728051 MS.gene039132:CDS 50.0%
CTCTCTCATCTCCGCAGCAT+CGG - chr2.2:22727294-22727313 MS.gene039132:CDS 55.0%
CTGCGGAGATGAGAGAGTGA+GGG + chr2.2:22727291-22727310 None:intergenic 55.0%
GAAGAGGAAGATTCCGGTGC+AGG - chr2.2:22727558-22727577 MS.gene039132:CDS 55.0%
GAGACACTGTTCCGATGCTG+CGG + chr2.2:22727308-22727327 None:intergenic 55.0%
GAGGACTGGGACTTGTAGTG+TGG - chr2.2:22727648-22727667 MS.gene039132:CDS 55.0%
GCCGTTGTTGCCGAGGTTAT+TGG - chr2.2:22727519-22727538 MS.gene039132:CDS 55.0%
! CTATTGTGCCGTTGTTGCCG+AGG - chr2.2:22727512-22727531 MS.gene039132:CDS 55.0%
! TACGGCTTTTGCGACGGTGT+CGG + chr2.2:22727125-22727144 None:intergenic 55.0%
CAAAAGGGGGGTGGCTGAGT+TGG - chr2.2:22728296-22728315 MS.gene039132:CDS 60.0%
GAGGAAGATTCCGGTGCAGG+TGG - chr2.2:22727561-22727580 MS.gene039132:CDS 60.0%
GCTGCGGAGATGAGAGAGTG+AGG + chr2.2:22727292-22727311 None:intergenic 60.0%
!! GGTGTCGGCGTTGCTGGTTA+CGG + chr2.2:22727110-22727129 None:intergenic 60.0%
!! TGCGATGGAGGGTGTTGTCG+AGG - chr2.2:22727726-22727745 MS.gene039132:CDS 60.0%
! GTCGGCGTTGCTGGTTACGG+TGG + chr2.2:22727107-22727126 None:intergenic 65.0%
!! TGCGACGGTGTCGGCGTTGC+TGG + chr2.2:22727116-22727135 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 22727100 22728455 22727100 ID=MS.gene039132
chr2.2 mRNA 22727100 22728455 22727100 ID=MS.gene039132.t1;Parent=MS.gene039132
chr2.2 exon 22727100 22728455 22727100 ID=MS.gene039132.t1.exon1;Parent=MS.gene039132.t1
chr2.2 CDS 22727100 22728455 22727100 ID=cds.MS.gene039132.t1;Parent=MS.gene039132.t1
Gene Sequence

>MS.gene039132

ATGGCCACCGTAACCAGCAACGCCGACACCGTCGCAAAAGCCGTAGACTCTCTTCTCAAATGGCGCAAATCACGATTAGAAACTGAAAAACCCAAACTCTTCGACGAAGACGAAGAATTCGTTTACCTCATCCTAACCCTAAAGAAAATTCCATCAAAAGGTCGCGTTAACCCTCACAAAATCCCAATCCCTCACTCTCTCATCTCCGCAGCATCGGAACAGTGTCTCATCATCGATGACAGAGCTAACAAACCCTCTCGTATCACCAAAGACGAAGCTCAGAAGAAAGTTCAATCGGAATCAATTCCTATTTCGAAGATTCTTAAGCTTTCGAAGCTCATGACGGATTATCGTCCGTTTGAGGCGAAAAGGAAGTTATGTAATTCTTATGATTTGTTTTTTGCTGATAAAGCTATTGTGCCGTTGTTGCCGAGGTTATTGGGGAAGCAGTTTTTTAAGAAGAGGAAGATTCCGGTGCAGGTGGATTTGCAGAAGAAGAATTGGAAGGAGCAGATTGAGAAGGCTTGTTCTTCTGCTTTGTTGTTTTTGAGGACTGGGACTTGTAGTGTGGTGAAGGTGGCTAAGTTGAGTATGGAGAGAGATGAGATTGTTGAGAATGTTGTTGCTGCGATGGAGGGTGTTGTCGAGGTTTTACCGAAGAAATGGGGGGTTGTTAGGTCTTTTCATGTTAAGTTGTTGGAGTCTCTTGCTTTGCCGGTTTATCAGGCTGTTCCTGATGTTAGGTTGAAGATTGAGGGGGTTAAGGATTTGGAGGATAAGTTGGTGATGGATGAGAAGAAGGTGAAAGACGCGAAGAAGAGTAAGAAGAAAGGTAGGATTCATGAGATTAAGTATATGGATGATAGAATGAGTTCAGATGGTGATGATCGCAGTGTTGATATTGATCTTGATGATGATATCGATGATGATTTTGTTAGTGGTGGTGGTGATGAGGTTGATGATGATAGTGAGATTGGTGATATCGAGAGTGGAGGGTTGGTGAGTAAGAAGAGGAAGAAAGGAGTGAAAAAAGAGGATTCACGTGAGTTGGGAAGTGTGAAATCATTGAAGGGTTCTGCTAAGAGGAAGAAGAAAGATGGGTTGAATGTGAATGCTGCAGAAGAAGGTTCAATTGAGAAGAGTGAAAAGAATAAGAAGTTATCCGTGAAGGAAAAGGGTTCAAAGAAGAAGAATAACAAAAGGGGGGTGGCTGAGTTGGCTGTGAAACATGCTCTTGAAGAAGTGCTCATGTTTAGCGGAAATGAATCATTGGTCAAGGATGAGGGTTCGGACAAGAAGAAGAGAAAGACGGATGATGTGAAGGTCAAGAGTAAGAAAAGTAAGAAGGCTGTATGA
Protein sequence

>MS.gene039132.t1

MATVTSNADTVAKAVDSLLKWRKSRLETEKPKLFDEDEEFVYLILTLKKIPSKGRVNPHKIPIPHSLISAASEQCLIIDDRANKPSRITKDEAQKKVQSESIPISKILKLSKLMTDYRPFEAKRKLCNSYDLFFADKAIVPLLPRLLGKQFFKKRKIPVQVDLQKKNWKEQIEKACSSALLFLRTGTCSVVKVAKLSMERDEIVENVVAAMEGVVEVLPKKWGVVRSFHVKLLESLALPVYQAVPDVRLKIEGVKDLEDKLVMDEKKVKDAKKSKKKGRIHEIKYMDDRMSSDGDDRSVDIDLDDDIDDDFVSGGGDEVDDDSEIGDIESGGLVSKKRKKGVKKEDSRELGSVKSLKGSAKRKKKDGLNVNAAEEGSIEKSEKNKKLSVKEKGSKKKNNKRGVAELAVKHALEEVLMFSGNESLVKDEGSDKKKRKTDDVKVKSKKSKKAV