AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058018

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058018.t1 MTR_4g075050 86.275 306 1 1 56 361 1 265 0.0 517
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058018.t1 AT5G61770 75.593 295 66 1 68 362 17 305 9.76e-164 462
MS.gene058018.t1 AT5G61770 76.109 293 64 1 68 360 17 303 1.83e-163 461
MS.gene058018.t1 AT5G61770 76.109 293 64 1 68 360 17 303 1.83e-163 461

Find 71 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTCCTCTTCGACGAATT+TGG 0.262207 4.2:-33672985 MS.gene058018:intron
TTATTCTTCTTGATAATAAC+AGG 0.299950 4.2:+33676606 None:intergenic
GGTATCTTCTTTCCAGTAAT+GGG 0.301868 4.2:+33676552 None:intergenic
AAACAGAGGAAGAATCAAAA+AGG 0.315897 4.2:-33676639 MS.gene058018:intron
CCTAGTATTGATCCCATTAC+TGG 0.323015 4.2:-33676564 MS.gene058018:CDS
AACAGCTTCAGATGGATCTT+AGG 0.328276 4.2:-33676485 MS.gene058018:CDS
GCCATCAAGTTCCTGATCTA+AGG 0.340037 4.2:-33673748 MS.gene058018:CDS
AGAGACTTTCTGAATGTTGC+TGG 0.344183 4.2:-33674706 MS.gene058018:CDS
GGGAGATATTAGAAGTATAT+CGG 0.357927 4.2:+33676965 None:intergenic
GGATGACTCTTCAACTAGTT+AGG 0.382251 4.2:-33673032 MS.gene058018:CDS
TGGTATCTTCTTTCCAGTAA+TGG 0.384814 4.2:+33676551 None:intergenic
TCTGAATGTTGCTGGACCTA+TGG 0.387257 4.2:-33674698 MS.gene058018:CDS
ATTCGCTTTGAAACACCTAT+AGG 0.388518 4.2:+33673788 None:intergenic
TCCTTAGATCAGGAACTTGA+TGG 0.390780 4.2:+33673747 None:intergenic
TTACGGCTGCAACTAGTGTT+AGG 0.391218 4.2:+33676925 None:intergenic
TTGAGACTGAGAGCAGTATA+AGG 0.391440 4.2:+33676450 None:intergenic
TGATTTGTAAGGGCTGGTTA+TGG 0.404504 4.2:-33673168 MS.gene058018:intron
GTGACTTTGTCTAGTAATCT+TGG 0.411075 4.2:-33673111 MS.gene058018:CDS
GAGAGTGCGAAGTTGGTTTA+CGG 0.412564 4.2:+33676908 None:intergenic
AATTAAGCAACACAATCCTT+TGG 0.413513 4.2:+33673864 None:intergenic
TCAATTAGATTACAACCTAT+AGG 0.417293 4.2:-33673803 MS.gene058018:CDS
TGAAGCTGTTTAAGAGGAAC+AGG 0.419611 4.2:+33676498 None:intergenic
TCCATCTGAAGCTGTTTAAG+AGG 0.421158 4.2:+33676492 None:intergenic
TAGGTTTCTTTCGCAGAGAA+TGG 0.427971 4.2:+33676944 None:intergenic
ATTGAGAAGGGACTGTGTTC+TGG 0.430812 4.2:-33673009 MS.gene058018:CDS
CTGAATGTTGCTGGACCTAT+GGG 0.440731 4.2:-33674697 MS.gene058018:CDS
CCAGTAATGGGATCAATACT+AGG 0.442643 4.2:+33676564 None:intergenic
TAAAACTTCACCTTACTTGA+GGG 0.447002 4.2:-33674644 MS.gene058018:CDS
TCTTGAAGATTCCTTAGATC+AGG 0.447271 4.2:+33673737 None:intergenic
TTGTCTGAGGTGGAAGATAT+CGG 0.467432 4.2:+33676835 None:intergenic
AGGTTTCTTTCGCAGAGAAT+GGG 0.474474 4.2:+33676945 None:intergenic
CTAAAACTTCACCTTACTTG+AGG 0.478099 4.2:-33674645 MS.gene058018:CDS
TCAATACTAGGCTGTGCCTC+TGG 0.481355 4.2:+33676576 None:intergenic
TATGGCAGAGAGTGCGAAGT+TGG 0.494831 4.2:+33676901 None:intergenic
ATGTAAGAGTAGGTCCTTGT+GGG 0.499307 4.2:+33674611 None:intergenic
GGAGATATTAGAAGTATATC+GGG 0.501028 4.2:+33676966 None:intergenic
GCTGTCAGACTTCAAGAGAT+TGG 0.502359 4.2:-33673060 MS.gene058018:CDS
TCTCTCTCCGTCTCCTGCGC+CGG 0.503479 4.2:-33676867 MS.gene058018:CDS
ACTAGTTGAAGAGTCATCCT+TGG 0.508593 4.2:+33673036 None:intergenic
AGAGAGACGACGGTGAGTTA+TGG 0.521251 4.2:+33676883 None:intergenic
TCCTCTTAAACAGCTTCAGA+TGG 0.521916 4.2:-33676493 MS.gene058018:CDS
GTAGTTGCAACCCTCAAGTA+AGG 0.522894 4.2:+33674634 None:intergenic
AAGCTTACCATGGTTGTCTG+AGG 0.549979 4.2:+33676822 None:intergenic
AGAAAGTGAAGCAGATGATG+AGG 0.552650 4.2:-33673142 MS.gene058018:CDS
TTATACTGCTCTCAGTCTCA+AGG 0.554961 4.2:-33676448 MS.gene058018:intron
TCAGTGGTGATTTACCTCTA+CGG 0.556377 4.2:-33674038 MS.gene058018:CDS
TATTAGAAGTATATCGGGCC+GGG 0.558094 4.2:+33676971 None:intergenic
TCAACTAGTTAGGATTGAGA+AGG 0.559747 4.2:-33673022 MS.gene058018:CDS
AGGAAATGTGTGACACCCAT+AGG 0.559784 4.2:+33674682 None:intergenic
TTAAACTCTCGTCTTGCCAA+AGG 0.560394 4.2:-33673880 MS.gene058018:CDS
TAAGATACAAGAGTATGCAT+TGG 0.562852 4.2:-33674587 MS.gene058018:CDS
GTGCTTTCTGGATTTGTCAG+TGG 0.563201 4.2:-33674054 MS.gene058018:CDS
CTTACCATGGTTGTCTGAGG+TGG 0.563723 4.2:+33676825 None:intergenic
TCGGAGATTAACACCGGCGC+AGG 0.563792 4.2:+33676854 None:intergenic
ATATTAGAAGTATATCGGGC+CGG 0.565994 4.2:+33676970 None:intergenic
ATTAACACCGGCGCAGGAGA+CGG 0.572479 4.2:+33676860 None:intergenic
AGCAGATGATGAGGCAGCAA+CGG 0.580635 4.2:-33673133 MS.gene058018:CDS
CAAATCCATGAAACATGACC+CGG 0.583582 4.2:-33676989 None:intergenic
ATGTTCGTTGTAAGCCGTAG+AGG 0.589274 4.2:+33674024 None:intergenic
TGTATCTTAAATGTAAGAGT+AGG 0.599693 4.2:+33674601 None:intergenic
CAACTAGTTAGGATTGAGAA+GGG 0.618382 4.2:-33673021 MS.gene058018:CDS
ATGTACCAAATTCGTCGAAG+AGG 0.628027 4.2:+33672980 None:intergenic
AATGTAAGAGTAGGTCCTTG+TGG 0.629934 4.2:+33674610 None:intergenic
GACTTCAAGAGATTGGTCCA+AGG 0.646494 4.2:-33673053 MS.gene058018:CDS
AGGGTTGCAACTACCCCACA+AGG 0.673136 4.2:-33674625 MS.gene058018:CDS
AAAAGATCTTTAGGGCATCG+AGG 0.675531 4.2:+33674535 None:intergenic
AAGATATCGGAGATTAACAC+CGG 0.677716 4.2:+33676848 None:intergenic
CAGGAGACGGAGAGAGACGA+CGG 0.683622 4.2:+33676873 None:intergenic
GAAGAATAACAAGCAACCAG+AGG 0.684893 4.2:-33676592 MS.gene058018:CDS
TCTTCCACCTCAGACAACCA+TGG 0.697883 4.2:-33676829 MS.gene058018:intron
TGTAAGAGTAGGTCCTTGTG+GGG 0.701027 4.2:+33674612 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTAAAAAGATCTTTA+GGG + chr4.2:33675327-33675346 None:intergenic 10.0%
!! ATTAATAATAATGCAAACAA+TGG + chr4.2:33676444-33676463 None:intergenic 15.0%
!! TTGATGTGATAAAATTTAAT+AGG + chr4.2:33675546-33675565 None:intergenic 15.0%
!! TTGTTTGCATTATTATTAAT+TGG - chr4.2:33676444-33676463 MS.gene058018:intron 15.0%
!!! GAATTTTTAAAAAGATCTTT+AGG + chr4.2:33675328-33675347 None:intergenic 15.0%
!! AAATAAGCTAAAAACAACTT+AGG - chr4.2:33673796-33673815 MS.gene058018:CDS 20.0%
!! AAATGAAGTAAAATTACTTG+AGG + chr4.2:33676912-33676931 None:intergenic 20.0%
!! AATAAGCTAAAAACAACTTA+GGG - chr4.2:33673797-33673816 MS.gene058018:CDS 20.0%
!! TAAGAAATTAGGAAAAATGA+AGG + chr4.2:33674871-33674890 None:intergenic 20.0%
!! TATTCAAATCTTAAACTCTA+TGG + chr4.2:33674948-33674967 None:intergenic 20.0%
!! TATTGGCAAACTAATTAAAT+GGG - chr4.2:33674904-33674923 MS.gene058018:intron 20.0%
!! TCTAGTGATAGTATTTAAAT+GGG - chr4.2:33675643-33675662 MS.gene058018:intron 20.0%
!! TTCTAGTGATAGTATTTAAA+TGG - chr4.2:33675642-33675661 MS.gene058018:intron 20.0%
!! TTCTCTCAATTTAAGAAATT+AGG + chr4.2:33674882-33674901 None:intergenic 20.0%
!!! AAAAGTTGGATTTTTTTTGA+TGG + chr4.2:33673143-33673162 None:intergenic 20.0%
!!! AAAAGTTTTGTTTTTTCAAG+AGG - chr4.2:33673323-33673342 MS.gene058018:intron 20.0%
!!! AAAGTTGGATTTTTTTTGAT+GGG + chr4.2:33673142-33673161 None:intergenic 20.0%
!!! AAAGTTTTGTTTTTTCAAGA+GGG - chr4.2:33673324-33673343 MS.gene058018:intron 20.0%
!!! AACATTTTAATTTAAGCGTT+TGG - chr4.2:33673677-33673696 MS.gene058018:intron 20.0%
!!! CTTGAAAAAACAAAACTTTT+TGG + chr4.2:33673323-33673342 None:intergenic 20.0%
!!! TAGAGTTTAAGATTTGAATA+CGG - chr4.2:33674948-33674967 MS.gene058018:intron 20.0%
!!! TCTCAACTAAAATAATTTTC+TGG - chr4.2:33674183-33674202 MS.gene058018:intron 20.0%
!!! TCTTTTTAAAAATTCTGCAT+TGG - chr4.2:33675333-33675352 MS.gene058018:intron 20.0%
!!! TTATTCTTCTTGATAATAAC+AGG + chr4.2:33673248-33673267 None:intergenic 20.0%
!!! TTCTGTAAAACAATGTTTTT+TGG - chr4.2:33674543-33674562 MS.gene058018:CDS 20.0%
!!! TTTTAGTTTTTGACAATATG+AGG + chr4.2:33675192-33675211 None:intergenic 20.0%
! ACTTTATGAAGGATTAACAT+GGG + chr4.2:33676265-33676284 None:intergenic 25.0%
! AGATCATAATTCATACATAG+TGG + chr4.2:33675721-33675740 None:intergenic 25.0%
! AGCTGATAATAACACAAAAA+TGG + chr4.2:33675601-33675620 None:intergenic 25.0%
! ATCCAACAACATTAAAAAGT+TGG + chr4.2:33673157-33673176 None:intergenic 25.0%
! ATTAGTTAAGACTGCTAAAT+GGG - chr4.2:33675517-33675536 MS.gene058018:intron 25.0%
! CAAATTTCAATTCTTCAACT+GGG - chr4.2:33673097-33673116 MS.gene058018:CDS 25.0%
! CAATTGTTGGAAAAATATTC+TGG + chr4.2:33675856-33675875 None:intergenic 25.0%
! CTAGTGATAGTATTTAAATG+GGG - chr4.2:33675644-33675663 MS.gene058018:intron 25.0%
! CTATTGGCAAACTAATTAAA+TGG - chr4.2:33674903-33674922 MS.gene058018:intron 25.0%
! CTTAAATTGAGAGAATCTAT+TGG - chr4.2:33674887-33674906 MS.gene058018:intron 25.0%
! GAAGAACAACAAACATAAAA+AGG + chr4.2:33673439-33673458 None:intergenic 25.0%
! GATAAGCTTAAATAAGACAA+TGG - chr4.2:33673864-33673883 MS.gene058018:CDS 25.0%
! GTTATAAACTAATCCAAACT+AGG + chr4.2:33674152-33674171 None:intergenic 25.0%
! TACAACCAAAAAAAAAAGAG+AGG + chr4.2:33676368-33676387 None:intergenic 25.0%
! TACTTTATGAAGGATTAACA+TGG + chr4.2:33676266-33676285 None:intergenic 25.0%
! TAGGACAATCAATCTAAATA+AGG + chr4.2:33674630-33674649 None:intergenic 25.0%
! TAGTTTAGTTATTTATGAGC+TGG - chr4.2:33674449-33674468 MS.gene058018:intron 25.0%
! TATTAAATCTAACTCGTAGA+TGG - chr4.2:33674472-33674491 MS.gene058018:intron 25.0%
! TCAAATTTCAATTCTTCAAC+TGG - chr4.2:33673096-33673115 MS.gene058018:CDS 25.0%
! TCAATTAGATTACAACCTAT+AGG - chr4.2:33676048-33676067 MS.gene058018:intron 25.0%
! TGTATCTTAAATGTAAGAGT+AGG + chr4.2:33675253-33675272 None:intergenic 25.0%
! TGTTGTAAAACAATGTTGTT+TGG - chr4.2:33674046-33674065 MS.gene058018:CDS 25.0%
! TTAAATAAGACAATGGAAAC+AGG - chr4.2:33673871-33673890 MS.gene058018:CDS 25.0%
!! AATTTCAGGGATTTTTGTTA+AGG - chr4.2:33674594-33674613 MS.gene058018:CDS 25.0%
!! ATCCAACTTTTTAATGTTGT+TGG - chr4.2:33673152-33673171 MS.gene058018:CDS 25.0%
!! ATTACTTGAGGAAAAAGTAT+AGG + chr4.2:33676900-33676919 None:intergenic 25.0%
!! TAAAAAAGAAGCATAAGCAA+TGG + chr4.2:33676640-33676659 None:intergenic 25.0%
!!! ATCATCCTCTCTTTTTTTTT+TGG - chr4.2:33676360-33676379 MS.gene058018:intron 25.0%
!!! TTTTCACTTGTGATTTGTAA+GGG - chr4.2:33676673-33676692 MS.gene058018:intron 25.0%
!!! TTTTTCACTTGTGATTTGTA+AGG - chr4.2:33676672-33676691 MS.gene058018:intron 25.0%
AAACAGAGGAAGAATCAAAA+AGG - chr4.2:33673212-33673231 MS.gene058018:intron 30.0%
AAACTGTTTATCAGCTATCA+TGG - chr4.2:33673531-33673550 MS.gene058018:intron 30.0%
AAATGAGCAAGTATCTTACT+TGG + chr4.2:33676157-33676176 None:intergenic 30.0%
AACAGAGGAAGAATCAAAAA+GGG - chr4.2:33673213-33673232 MS.gene058018:intron 30.0%
AATGCAAATGAGATACATCT+AGG + chr4.2:33673898-33673917 None:intergenic 30.0%
AATTAAGCAACACAATCCTT+TGG + chr4.2:33675990-33676009 None:intergenic 30.0%
ACTCTAAATTAACAAGAGAG+TGG + chr4.2:33673066-33673085 None:intergenic 30.0%
CATTAGTTAAGACTGCTAAA+TGG - chr4.2:33675516-33675535 MS.gene058018:intron 30.0%
CTACGAATTAGATTTAACAC+AGG + chr4.2:33673974-33673993 None:intergenic 30.0%
CTGTGTTAAATCTAATTCGT+AGG - chr4.2:33673972-33673991 MS.gene058018:intron 30.0%
CTTATTTCAACATACATGAG+AGG - chr4.2:33674760-33674779 MS.gene058018:intron 30.0%
GACAATGAAATGTTATGTTG+TGG - chr4.2:33674422-33674441 MS.gene058018:intron 30.0%
GTTTACTTCTGAATGACTTT+GGG + chr4.2:33676590-33676609 None:intergenic 30.0%
GTTTGTAGCTCATACAAAAA+CGG + chr4.2:33673744-33673763 None:intergenic 30.0%
TAAAACTTCACCTTACTTGA+GGG - chr4.2:33675207-33675226 MS.gene058018:intron 30.0%
TAGGACAATCAATCTAAACA+AGG + chr4.2:33674133-33674152 None:intergenic 30.0%
TATATCTTCCTTTCTCAATC+AGG + chr4.2:33676493-33676512 None:intergenic 30.0%
TCACGTTAGATAACTACTAA+TGG + chr4.2:33675749-33675768 None:intergenic 30.0%
TGTTAAATCTAATTCGTAGG+TGG - chr4.2:33673975-33673994 MS.gene058018:intron 30.0%
TGTTTACTTCTGAATGACTT+TGG + chr4.2:33676591-33676610 None:intergenic 30.0%
TTAGATTGATTGTCCTAGTT+TGG - chr4.2:33674136-33674155 MS.gene058018:intron 30.0%
TTGTCACATGTGATTGAAAT+CGG + chr4.2:33675051-33675070 None:intergenic 30.0%
! ATTATTTTCGTAAGCTATCC+AGG + chr4.2:33674276-33674295 None:intergenic 30.0%
! CTATTTTGCAGGAAAAGAAA+CGG - chr4.2:33675113-33675132 MS.gene058018:intron 30.0%
! GATTTCAGGGATTTTTGTTA+AGG - chr4.2:33674097-33674116 MS.gene058018:intron 30.0%
! GCTTGCTAAAAACAACTTAT+AGG - chr4.2:33673557-33673576 MS.gene058018:intron 30.0%
! GGAGATATTAGAAGTATATC+GGG + chr4.2:33672888-33672907 None:intergenic 30.0%
! GGGAGATATTAGAAGTATAT+CGG + chr4.2:33672889-33672908 None:intergenic 30.0%
! TAAGATACAAGAGTATGCAT+TGG - chr4.2:33675264-33675283 MS.gene058018:intron 30.0%
! TGAGTGCTGTATTAATTTCA+GGG - chr4.2:33674581-33674600 MS.gene058018:CDS 30.0%
! TGTTTGGATTAATGGGTAAT+GGG - chr4.2:33676410-33676429 MS.gene058018:intron 30.0%
! TTAACCTTTTATTCCTATCG+CGG - chr4.2:33675474-33675493 MS.gene058018:intron 30.0%
! TTATTTTCGTAAGCTATCCA+GGG + chr4.2:33673782-33673801 None:intergenic 30.0%
! TTCATGAGTTTGGAGATTTT+TGG - chr4.2:33674009-33674028 MS.gene058018:CDS 30.0%
!! ACAGTTTTAACGTCAATTGT+TGG + chr4.2:33675869-33675888 None:intergenic 30.0%
!! TTAAGGTGTTTTCATGAGTT+TGG - chr4.2:33674496-33674515 MS.gene058018:intron 30.0%
!! TTTTGTTGTGAATGAAACAG+AGG - chr4.2:33673198-33673217 MS.gene058018:intron 30.0%
!!! AGACTAAGCTCATTGATTTT+CGG - chr4.2:33676019-33676038 MS.gene058018:intron 30.0%
!!! TTTTTTTTGGTTGTAACTCG+TGG - chr4.2:33676373-33676392 MS.gene058018:intron 30.0%
AAATGAGGTTAAGCTTACCA+TGG + chr4.2:33673042-33673061 None:intergenic 35.0%
AAGATATCGGAGATTAACAC+CGG + chr4.2:33673006-33673025 None:intergenic 35.0%
AATATCAGCCTGATTGAGAA+AGG - chr4.2:33676482-33676501 MS.gene058018:CDS 35.0%
AGAGGTAATTTGAATGAGAG+AGG - chr4.2:33674715-33674734 MS.gene058018:CDS 35.0%
AGTCTTAACTGATTGCAAAC+GGG + chr4.2:33675677-33675696 None:intergenic 35.0%
CAACTAGTTAGGATTGAGAA+GGG - chr4.2:33676830-33676849 MS.gene058018:CDS 35.0%
CAGACTTGCATACTTTATGA+AGG + chr4.2:33676276-33676295 None:intergenic 35.0%
CAGTCTTAACTGATTGCAAA+CGG + chr4.2:33675678-33675697 None:intergenic 35.0%
CTAAAACTTCACCTTACTTG+AGG - chr4.2:33675206-33675225 MS.gene058018:intron 35.0%
CTACAAGAGTCAATTTCTCT+GGG + chr4.2:33674991-33675010 None:intergenic 35.0%
CTTGAGAGAACTTATGGAAA+GGG + chr4.2:33673594-33673613 None:intergenic 35.0%
GAGCTTATGACATCTCTATA+AGG + chr4.2:33674309-33674328 None:intergenic 35.0%
GATTTCAATCACATGTGACA+AGG - chr4.2:33675050-33675069 MS.gene058018:intron 35.0%
GGTATCTTCTTTCCAGTAAT+GGG + chr4.2:33673302-33673321 None:intergenic 35.0%
GTAGTTATCTAACGTGATGT+TGG - chr4.2:33675752-33675771 MS.gene058018:intron 35.0%
GTGACTTTGTCTAGTAATCT+TGG - chr4.2:33676740-33676759 MS.gene058018:intron 35.0%
GTTTAACCTGAAATATACCG+CGG + chr4.2:33675957-33675976 None:intergenic 35.0%
TAACAAGAGAGTGGTAAATG+AGG + chr4.2:33673057-33673076 None:intergenic 35.0%
TAATGTTGTTGGATTGAGCT+TGG - chr4.2:33673163-33673182 MS.gene058018:intron 35.0%
TATTGAGAAGCTTGTCATAC+TGG - chr4.2:33674845-33674864 MS.gene058018:intron 35.0%
TCAAATAAGCCAGTTGAAAC+AGG - chr4.2:33674367-33674386 MS.gene058018:intron 35.0%
TCAACTAGTTAGGATTGAGA+AGG - chr4.2:33676829-33676848 MS.gene058018:intron 35.0%
TCTTGAAGATTCCTTAGATC+AGG + chr4.2:33676117-33676136 None:intergenic 35.0%
TGCAATGCTAATGACAGAAA+AGG + chr4.2:33676242-33676261 None:intergenic 35.0%
TGCTAATGAAATGCGAGAAA+GGG - chr4.2:33675435-33675454 MS.gene058018:intron 35.0%
TGGTATCTTCTTTCCAGTAA+TGG + chr4.2:33673303-33673322 None:intergenic 35.0%
TTAAGATGGCTTCATGAGTT+TGG - chr4.2:33673999-33674018 MS.gene058018:CDS 35.0%
TTGCTAATGAAATGCGAGAA+AGG - chr4.2:33675434-33675453 MS.gene058018:intron 35.0%
! AAGGGTGTGTTTGGATTAAT+GGG - chr4.2:33676403-33676422 MS.gene058018:intron 35.0%
! AGACAGTGAAATGTTTGTTG+TGG - chr4.2:33673925-33673944 MS.gene058018:intron 35.0%
! ATATTAGAAGTATATCGGGC+CGG + chr4.2:33672884-33672903 None:intergenic 35.0%
! CAGAGAAATTGACTCTTGTA+GGG - chr4.2:33674990-33675009 MS.gene058018:intron 35.0%
! CGTTTCTTTTCCTGCAAAAT+AGG + chr4.2:33675115-33675134 None:intergenic 35.0%
! CTATCATCTTTTACTGTCTC+AGG - chr4.2:33676962-33676981 MS.gene058018:CDS 35.0%
! GTAACTCGTGGTTTTTCTAA+GGG - chr4.2:33676385-33676404 MS.gene058018:intron 35.0%
! GTGAGTGCTGTATTAATTTC+AGG - chr4.2:33674580-33674599 MS.gene058018:CDS 35.0%
! GTGTTTGGATTAATGGGTAA+TGG - chr4.2:33676409-33676428 MS.gene058018:intron 35.0%
! TAAGGGTGTGTTTGGATTAA+TGG - chr4.2:33676402-33676421 MS.gene058018:intron 35.0%
! TGTAACTCGTGGTTTTTCTA+AGG - chr4.2:33676384-33676403 MS.gene058018:intron 35.0%
! TTTTACCAGATTGTGCTTTC+TGG - chr4.2:33675785-33675804 MS.gene058018:intron 35.0%
!! AAATGAGCTATGGCTTTGTT+TGG - chr4.2:33673651-33673670 MS.gene058018:intron 35.0%
!! AATTCGTAGGTGGATTAAGA+TGG - chr4.2:33673985-33674004 MS.gene058018:intron 35.0%
!! ATTCGCTTTGAAACACCTAT+AGG + chr4.2:33676066-33676085 None:intergenic 35.0%
!! CTTATTTTCGTAAGCTATCC+AGG + chr4.2:33673783-33673802 None:intergenic 35.0%
!! TCTAACTCGTAGATGGATTA+AGG - chr4.2:33674479-33674498 MS.gene058018:intron 35.0%
!! TGAGTGCTGTATTGATTTCA+GGG - chr4.2:33674084-33674103 MS.gene058018:intron 35.0%
!! TTCATGAGTTTGGAGTCTTT+TGG - chr4.2:33674506-33674525 MS.gene058018:intron 35.0%
!!! TTCTGGTTTTTACTCTCGAA+AGG - chr4.2:33674200-33674219 MS.gene058018:intron 35.0%
AACAGCTTCAGATGGATCTT+AGG - chr4.2:33673366-33673385 MS.gene058018:intron 40.0%
AATGTAAGAGTAGGTCCTTG+TGG + chr4.2:33675244-33675263 None:intergenic 40.0%
ACACAAGTGCTTATGTGAGT+AGG - chr4.2:33673621-33673640 MS.gene058018:intron 40.0%
ACTAGTTGAAGAGTCATCCT+TGG + chr4.2:33676818-33676837 None:intergenic 40.0%
AGAAAGTGAAGCAGATGATG+AGG - chr4.2:33676709-33676728 MS.gene058018:intron 40.0%
AGACAGCTTGAGAGAACTTA+TGG + chr4.2:33673600-33673619 None:intergenic 40.0%
AGAGACTTTCTGAATGTTGC+TGG - chr4.2:33675145-33675164 MS.gene058018:intron 40.0%
AGGTAAGCTCAAATGAGCTA+TGG - chr4.2:33673641-33673660 MS.gene058018:intron 40.0%
AGGTTTCTTTCGCAGAGAAT+GGG + chr4.2:33672909-33672928 None:intergenic 40.0%
ATGTAAGAGTAGGTCCTTGT+GGG + chr4.2:33675243-33675262 None:intergenic 40.0%
CAACATACATGAGAGGCTAT+TGG - chr4.2:33674767-33674786 MS.gene058018:intron 40.0%
CCTACAAGAGTCAATTTCTC+TGG + chr4.2:33674992-33675011 None:intergenic 40.0%
CTATCGCGGTGAAGAATTAA+GGG - chr4.2:33675488-33675507 MS.gene058018:intron 40.0%
GAAGAATAACAAGCAACCAG+AGG - chr4.2:33673259-33673278 MS.gene058018:intron 40.0%
GCTTGAGAGAACTTATGGAA+AGG + chr4.2:33673595-33673614 None:intergenic 40.0%
GGATGACTCTTCAACTAGTT+AGG - chr4.2:33676819-33676838 MS.gene058018:intron 40.0%
TAGGTTTCTTTCGCAGAGAA+TGG + chr4.2:33672910-33672929 None:intergenic 40.0%
TCAGTGGTGATTTACCTCTA+CGG - chr4.2:33675813-33675832 MS.gene058018:intron 40.0%
TCCTCTTAAACAGCTTCAGA+TGG - chr4.2:33673358-33673377 MS.gene058018:intron 40.0%
TCCTTAGATCAGGAACTTGA+TGG + chr4.2:33676107-33676126 None:intergenic 40.0%
TGAAGCTGTTTAAGAGGAAC+AGG + chr4.2:33673356-33673375 None:intergenic 40.0%
TTAAACTCTCGTCTTGCCAA+AGG - chr4.2:33675971-33675990 MS.gene058018:intron 40.0%
TTATACTGCTCTCAGTCTCA+AGG - chr4.2:33673403-33673422 MS.gene058018:intron 40.0%
TTCAATCACATGTGACAAGG+TGG - chr4.2:33675053-33675072 MS.gene058018:intron 40.0%
TTCACCGCGATAGGAATAAA+AGG + chr4.2:33675481-33675500 None:intergenic 40.0%
TTGAGACTGAGAGCAGTATA+AGG + chr4.2:33673404-33673423 None:intergenic 40.0%
! CAAATCCAGAAAGCACAATC+TGG + chr4.2:33675793-33675812 None:intergenic 40.0%
! CCAGAGAAATTGACTCTTGT+AGG - chr4.2:33674989-33675008 MS.gene058018:intron 40.0%
! CCAGTAATGGGATCAATACT+AGG + chr4.2:33673290-33673309 None:intergenic 40.0%
! TATTAGAAGTATATCGGGCC+GGG + chr4.2:33672883-33672902 None:intergenic 40.0%
! TCCATCTGAAGCTGTTTAAG+AGG + chr4.2:33673362-33673381 None:intergenic 40.0%
! TCTCATGTCTCCTATTTTGC+AGG - chr4.2:33675102-33675121 MS.gene058018:intron 40.0%
! TTGTCTGAGGTGGAAGATAT+CGG + chr4.2:33673019-33673038 None:intergenic 40.0%
!! AAAAGATCTTTAGGGCATCG+AGG + chr4.2:33675319-33675338 None:intergenic 40.0%
!! CCTAGTATTGATCCCATTAC+TGG - chr4.2:33673287-33673306 MS.gene058018:intron 40.0%
!! GGTTTTTCTAAGGGTGTGTT+TGG - chr4.2:33676394-33676413 MS.gene058018:intron 40.0%
!! GTGAGTGCTGTATTGATTTC+AGG - chr4.2:33674083-33674102 MS.gene058018:intron 40.0%
!! TGATTTGTAAGGGCTGGTTA+TGG - chr4.2:33676683-33676702 MS.gene058018:intron 40.0%
!!! TTCTGGTTTTTACTCTCGAG+AGG - chr4.2:33674697-33674716 MS.gene058018:CDS 40.0%
AAGCTTACCATGGTTGTCTG+AGG + chr4.2:33673032-33673051 None:intergenic 45.0%
AGCCAGTTGAAACAGGTCTT+AGG - chr4.2:33674374-33674393 MS.gene058018:intron 45.0%
AGGAAATGTGTGACACCCAT+AGG + chr4.2:33675172-33675191 None:intergenic 45.0%
ATGTTCGTTGTAAGCCGTAG+AGG + chr4.2:33675830-33675849 None:intergenic 45.0%
ATTGAGAAGGGACTGTGTTC+TGG - chr4.2:33676842-33676861 MS.gene058018:CDS 45.0%
CACCTAAGACCTGTTTCAAC+TGG + chr4.2:33674379-33674398 None:intergenic 45.0%
CCTATCGCGGTGAAGAATTA+AGG - chr4.2:33675487-33675506 MS.gene058018:intron 45.0%
CCTTAATTCTTCACCGCGAT+AGG + chr4.2:33675490-33675509 None:intergenic 45.0%
CTAATGCCGCGGTATATTTC+AGG - chr4.2:33675948-33675967 MS.gene058018:intron 45.0%
CTGAAATATACCGCGGCATT+AGG + chr4.2:33675950-33675969 None:intergenic 45.0%
CTGAATGTTGCTGGACCTAT+GGG - chr4.2:33675154-33675173 MS.gene058018:intron 45.0%
GCCATCAAGTTCCTGATCTA+AGG - chr4.2:33676103-33676122 MS.gene058018:intron 45.0%
GCTGTCAGACTTCAAGAGAT+TGG - chr4.2:33676791-33676810 MS.gene058018:intron 45.0%
GTAGTTGCAACCCTCAAGTA+AGG + chr4.2:33675220-33675239 None:intergenic 45.0%
GTGCTTTCTGGATTTGTCAG+TGG - chr4.2:33675797-33675816 MS.gene058018:intron 45.0%
TCTGAATGTTGCTGGACCTA+TGG - chr4.2:33675153-33675172 MS.gene058018:intron 45.0%
TGTAAGAGTAGGTCCTTGTG+GGG + chr4.2:33675242-33675261 None:intergenic 45.0%
TTACGGCTGCAACTAGTGTT+AGG + chr4.2:33672929-33672948 None:intergenic 45.0%
! ACTATTTTCGTAAGCTGCCC+TGG - chr4.2:33674255-33674274 MS.gene058018:intron 45.0%
! ACTGTTTTCGTAAGCTGTCC+TGG - chr4.2:33673762-33673781 MS.gene058018:CDS 45.0%
! CACTTGTGATTTGTAAGGGC+TGG - chr4.2:33676677-33676696 MS.gene058018:intron 45.0%
! GACTTCAAGAGATTGGTCCA+AGG - chr4.2:33676798-33676817 MS.gene058018:intron 45.0%
! GAGAGTGCGAAGTTGGTTTA+CGG + chr4.2:33672946-33672965 None:intergenic 45.0%
!! GGATTAATGGGTAATGGGAC+AGG - chr4.2:33676415-33676434 MS.gene058018:intron 45.0%
AAGTCGATCTCCTAATGCCG+CGG - chr4.2:33675937-33675956 MS.gene058018:intron 50.0%
AGAGAGACGACGGTGAGTTA+TGG + chr4.2:33672971-33672990 None:intergenic 50.0%
AGCAGATGATGAGGCAGCAA+CGG - chr4.2:33676718-33676737 MS.gene058018:intron 50.0%
CTTACCATGGTTGTCTGAGG+TGG + chr4.2:33673029-33673048 None:intergenic 50.0%
TCAATACTAGGCTGTGCCTC+TGG + chr4.2:33673278-33673297 None:intergenic 50.0%
TCTTCCACCTCAGACAACCA+TGG - chr4.2:33673022-33673041 MS.gene058018:CDS 50.0%
! TATGGCAGAGAGTGCGAAGT+TGG + chr4.2:33672953-33672972 None:intergenic 50.0%
AGGGTTGCAACTACCCCACA+AGG - chr4.2:33675226-33675245 MS.gene058018:intron 55.0%
ATTAACACCGGCGCAGGAGA+CGG + chr4.2:33672994-33673013 None:intergenic 55.0%
CAGGAGACGGAGAGAGACGA+CGG + chr4.2:33672981-33673000 None:intergenic 60.0%
TCGGAGATTAACACCGGCGC+AGG + chr4.2:33673000-33673019 None:intergenic 60.0%
TCTCTCTCCGTCTCCTGCGC+CGG - chr4.2:33672984-33673003 MS.gene058018:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 33672876 33676997 33672876 ID=MS.gene058018
chr4.2 mRNA 33672876 33676997 33672876 ID=MS.gene058018.t1;Parent=MS.gene058018
chr4.2 exon 33676830 33676997 33676830 ID=MS.gene058018.t1.exon1;Parent=MS.gene058018.t1
chr4.2 CDS 33676830 33676997 33676830 ID=cds.MS.gene058018.t1;Parent=MS.gene058018.t1
chr4.2 exon 33676449 33676655 33676449 ID=MS.gene058018.t1.exon2;Parent=MS.gene058018.t1
chr4.2 CDS 33676449 33676655 33676449 ID=cds.MS.gene058018.t1;Parent=MS.gene058018.t1
chr4.2 exon 33674519 33674749 33674519 ID=MS.gene058018.t1.exon3;Parent=MS.gene058018.t1
chr4.2 CDS 33674519 33674749 33674519 ID=cds.MS.gene058018.t1;Parent=MS.gene058018.t1
chr4.2 exon 33673987 33674079 33673987 ID=MS.gene058018.t1.exon4;Parent=MS.gene058018.t1
chr4.2 CDS 33673987 33674079 33673987 ID=cds.MS.gene058018.t1;Parent=MS.gene058018.t1
chr4.2 exon 33673716 33673903 33673716 ID=MS.gene058018.t1.exon5;Parent=MS.gene058018.t1
chr4.2 CDS 33673716 33673903 33673716 ID=cds.MS.gene058018.t1;Parent=MS.gene058018.t1
chr4.2 exon 33672986 33673179 33672986 ID=MS.gene058018.t1.exon6;Parent=MS.gene058018.t1
chr4.2 CDS 33672986 33673179 33672986 ID=cds.MS.gene058018.t1;Parent=MS.gene058018.t1
chr4.2 exon 33672876 33672889 33672876 ID=MS.gene058018.t1.exon7;Parent=MS.gene058018.t1
chr4.2 CDS 33672876 33672889 33672876 ID=cds.MS.gene058018.t1;Parent=MS.gene058018.t1
Gene Sequence

>MS.gene058018

ATGACCCGGCCCGATATACTTCTAATATCTCCCATTCTCTGCGAAAGAAACCTAACACTAGTTGCAGCCGTAAACCAACTTCGCACTCTCTGCCATAACTCACCGTCGTCTCTCTCCGTCTCCTGCGCCGGTGTTAATCTCCGATATCTTCCACCTCAGACAACCATGGTAAGCTTAACCTCATTTACCACTCTCTTGTTAATTTAGAGTTTATATGTTTTCAAATTTCAATTCTTCAACTGGGTATTGCTTTTTATCTCAAACCCATCAAAAAAAATCCAACTTTTTAATGTTGTTGGATTGAGCTTGGAAATTTACACTGTTTTGTTGTGAATGAAACAGAGGAAGAATCAAAAAGGGTTTAGAAGACCTGTTATTATCAAGAAGAATAACAAGCAACCAGAGGCACAGCCTAGTATTGATCCCATTACTGGAAAGAAGATACCAAAAAGTTTTGTTTTTTCAAGAGGGAAGTTACCTGTTCCTCTTAAACAGCTTCAGATGGATCTTAGGAAGTTGATGCTTCCTTATACTGCTCTCAGTCTCAAGGTTAATATCTTCCTTTTTATGTTTGTTGTTCTTCTTTGATATAAGTGTTTATCATTATAAGTGCTTATGTATAAGTTATTTATATAACAAAAGATAAAATAAAGTTAAACTGTTTATCAGCTATCATGGATAGCTTGCTAAAAACAACTTATAGGCATGTCATGAGCCCTTTCCATAAGTTCTCTCAAGCTGTCTCACACAAGTGCTTATGTGAGTAGGTAAGCTCAAATGAGCTATGGCTTTGTTTGGATAAACATTTTAATTTAAGCGTTTGGCGTATAAGTTTTTTTATAACAAAAAATAAAATAAAGTTCAACCGTTTTTGTATGAGCTACAAACTGTTTTCGTAAGCTGTCCTGGATAGCTTACGAAAATAAGCTAAAAACAACTTAGGGATGTCATAAGCTCGTTTCGTAAGCTCTCTGTGTGAATGCTACTAGATAAGCTTAAATAAGACAATGGAAACAGGTCCTAGATGTATCTCATTTGCATTGCTTTGCAGACAGTGAAATGTTTGTTGTGGAGAGTACTTTAGTTATTTATGAGCCTGTGTTAAATCTAATTCGTAGGTGGATTAAGATGGCTTCATGAGTTTGGAGATTTTTGGAGTGATTAATTAACTGTTGTAAAACAATGTTGTTTGGAGAATGCTGTGACAGTGAGTGCTGTATTGATTTCAGGGATTTTTGTTAAGGCAGTGTGCTGCCTTGTTTAGATTGATTGTCCTAGTTTGGATTAGTTTATAACTTATGAGAGTTTCTCAACTAAAATAATTTTCTGGTTTTTACTCTCGAAAGGTAATTTTCAACTGTTTTTTGTTTAAGCTACAAACTATTTTCGTAAGCTGCCCTGGATAGCTTACGAAAATAATCTGAAAACAGCCTTATAGAGATGTCATAAGCTCGTTTCGTAAGCTCTCTGTGTGAATGCTACTAGATAAGCTCAAATAAGCCAGTTGAAACAGGTCTTAGGTGTATCTCATTTGCATTGCTGTGAAGACAATGAAATGTTATGTTGTGGTGAGTAGTTTAGTTATTTATGAGCTGGTATTAAATCTAACTCGTAGATGGATTAAGGTGTTTTCATGAGTTTGGAGTCTTTTGGAGTGATTAATGAACTTCTGTAAAACAATGTTTTTTGGAGAATGCTGTGACAGTGAGTGCTGTATTAATTTCAGGGATTTTTGTTAAGGCAATGTGCTGCCTTATTTAGATTGATTGTCCTAGTTTGGATTAGTTTATAACTTATGAGAGTTTCTCAACTAAAATAATTTTCTGGTTTTTACTCTCGAGAGGTAATTTGAATGAGAGAGGTGAAAAAGTTCAAATACTGAAACTTATTTCAACATACATGAGAGGCTATTGGTAAAACATTATCACATCTTACACGATTCTTTAATATCTCTTTTTTCATAAGTGCTTATTGAGAAGCTTGTCATACTGGCCTTCATTTTTCCTAATTTCTTAAATTGAGAGAATCTATTGGCAAACTAATTAAATGGGAAAAAGTTTACTGTTATGCCATAGAGTTTAAGATTTGAATACGGTAGCATGTTAAAATTTACCCAGAGAAATTGACTCTTGTAGGGATTTTTAGTTGTCACGCAATATAATACATTTTTAACCGATTTCAATCACATGTGACAAGGTGGCATCTATGCTAGTGTTGACTATATACTCTCATGTCTCCTATTTTGCAGGAAAAGAAACGGAATAATCTGAGAGACTTTCTGAATGTTGCTGGACCTATGGGTGTCACACATTTCCTCATATTGTCAAAAACTAAAACTTCACCTTACTTGAGGGTTGCAACTACCCCACAAGGACCTACTCTTACATTTAAGATACAAGAGTATGCATTGGCTGCTGATATTGCTCGATCTCAATTGCATCCTCGATGCCCTAAAGATCTTTTTAAAAATTCTGCATTGGTAAAGTATCTCTTTATGCTTATCGACATTAAAATTTAGATAATGAAAAACAGAATGTCAGAAGAAATCATCACTAGATTTGCTAATGAAATGCGAGAAAGGGATTGTATATTATATTTTTAACCTTTTATTCCTATCGCGGTGAAGAATTAAGGGATAGTCATTAGTTAAGACTGCTAAATGGGTGTCCTATTAAATTTTATCACATCAAACAGAAAAATCTATTCAATGTCTTCGTGATGTCCATTTTTGTGTTATTATCAGCTTCTCATGTGACTTAACTGCTCTTCTAGTGATAGTATTTAAATGGGGAATATATCCCGTTTGCAATCAGTTAAGACTGACATTAAAATATGTATTTATCCACTATGTATGAATTATGATCTAGTAACCATTAGTAGTTATCTAACGTGATGTTGGCATGTTTTTATTTTACCAGATTGTGCTTTCTGGATTTGTCAGTGGTGATTTACCTCTACGGCTTACAACGAACATGTTCCAGAATATTTTTCCAACAATTGACGTTAAAACTGTGAGTTGTACAGACAAAAACACTTTAAAAACTTAATTAATTAAAACTGTAAGTCGATCTCCTAATGCCGCGGTATATTTCAGGTTAAACTCTCGTCTTGCCAAAGGATTGTGTTGCTTAATTACAACAAAGAGACTAAGCTCATTGATTTTCGGCATTATTCAATTAGATTACAACCTATAGGTGTTTCAAAGCGAATTAGAAAACTTGTTCAAAGCCATCAAGTTCCTGATCTAAGGAATCTTCAAGACGTGAGTGATTTTGTCACCAAGTAAGATACTTGCTCATTTACATAAGATTTCATTTTTTATTTTCTTTATGAATATTAACTTGAAGATAGAGATAATATAATCCTTTTCTGTCATTAGCATTGCACCCATGTTAATCCTTCATAAAGTATGCAAGTCTGTTATTTCATAGTATTGAGATGTGTAATGAAGAGTGTCGTATATAATCTAACATGCACTTAGTTCATCATCCTCTCTTTTTTTTTTGGTTGTAACTCGTGGTTTTTCTAAGGGTGTGTTTGGATTAATGGGTAATGGGACAGGATTCCATTGTTTGCATTATTATTAATTGGTAGCTTAGAACTTAAAATATCAGCCTGATTGAGAAAGGAAGATATATTTGCTTAAAATTTTAGCATGCAATCAACATTCATATTTCTATGTCTGTAGCAGCTATAAACATATAAATAAAGCCCAAAGTCATTCAGAAGTAAACAAAAATTTATTTATACAAAATAATTAACCATTGCTTATGCTTCTTTTTTATTTTATTCTTATTTTTTCACTTGTGATTTGTAAGGGCTGGTTATGGATCAGAAAGTGAAGCAGATGATGAGGCAGCAACGGTGACTTTGTCTAGTAATCTTGGTAGAGTTAATCGTGCTTCTACAAAAAGTGCTGTCAGACTTCAAGAGATTGGTCCAAGGATGACTCTTCAACTAGTTAGGATTGAGAAGGGACTGTGTTCTGGTGAAGTCCTCTTCGACGAATTTGGTACATCATCCTATACTTTTTCCTCAAGTAATTTTACTTCATTTTCATTGAGAAATTTATGAAGTATCATGTCACTATCATCTTTTACTGTCTCAGGCATATATGTGTGA
Protein sequence

>MS.gene058018.t1

MTRPDILLISPILCERNLTLVAAVNQLRTLCHNSPSSLSVSCAGVNLRYLPPQTTMRKNQKGFRRPVIIKKNNKQPEAQPSIDPITGKKIPKSFVFSRGKLPVPLKQLQMDLRKLMLPYTALSLKEKKRNNLRDFLNVAGPMGVTHFLILSKTKTSPYLRVATTPQGPTLTFKIQEYALAADIARSQLHPRCPKDLFKNSALIVLSGFVSGDLPLRLTTNMFQNIFPTIDVKTVKLSSCQRIVLLNYNKETKLIDFRHYSIRLQPIGVSKRIRKLVQSHQVPDLRNLQDVSDFVTKAGYGSESEADDEAATVTLSSNLGRVNRASTKSAVRLQEIGPRMTLQLVRIEKGLCSGEVLFDEFGIYV