AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028002


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028002.t1 MTR_5g028350 97.232 289 8 0 1 289 1 289 0.0 568
MS.gene028002.t1 MTR_8g075400 76.552 290 37 4 1 289 14 273 2.26e-139 394
MS.gene028002.t1 MTR_8g075400 87.500 224 26 1 1 224 1 222 6.17e-130 369
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028002.t1 AT3G22660 65.529 293 86 6 2 290 12 293 3.65e-126 361

Find 71 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGCCTCCAAAACCAAATT+TGG 0.189093 5.2:+19259154 None:intergenic
GCTGAAACAACTAATGATTT+TGG 0.204488 5.2:-19259107 MS.gene028002:CDS
GATACTAAGAATGCCAAATT+TGG 0.209577 5.2:-19259167 MS.gene028002:CDS
AAGAATGCCAAATTTGGTTT+TGG 0.274042 5.2:-19259161 MS.gene028002:CDS
ATGGAGGTGCCTTTGATTAA+TGG 0.295822 5.2:-19259908 MS.gene028002:CDS
TCAAAGCTCAAAGACTTATC+TGG 0.326167 5.2:+19259308 None:intergenic
AGAAAGGGCCAAGCAGAAAA+AGG 0.351345 5.2:-19259402 MS.gene028002:CDS
TCAGTTTCACCGTCAATCAT+TGG 0.370634 5.2:+19259881 None:intergenic
TCTGCTTCTTCGATCCCTTC+CGG 0.371874 5.2:+19259135 None:intergenic
TGCTTCCAATCCACATTCTC+AGG 0.372391 5.2:+19259731 None:intergenic
TGACGGTGAAACTGAAGATT+TGG 0.372926 5.2:-19259873 MS.gene028002:CDS
TTCAGTAAGGGTTCTGCTGC+TGG 0.381325 5.2:-19259080 MS.gene028002:CDS
TTACCTCCTGACCGATCTCC+TGG 0.396701 5.2:+19259236 None:intergenic
AGACAATCTCTTTGCTTCCC+TGG 0.401049 5.2:+19259451 None:intergenic
AGATAAGTCTTTGAGCTTTG+AGG 0.404345 5.2:-19259306 MS.gene028002:CDS
CTGAAGAGAGAAGAAAGGCC+AGG 0.422247 5.2:-19259469 MS.gene028002:CDS
CTCTCCATTGATATTGATCA+AGG 0.429280 5.2:-19259704 MS.gene028002:CDS
TCTTCTGAATCTGATTCAAA+TGG 0.456186 5.2:+19259836 None:intergenic
AGTCACAGAAATTGAAAGAA+AGG 0.458750 5.2:-19259418 MS.gene028002:CDS
TAGTCAGCAGGTCTCAAGAA+AGG 0.478435 5.2:+19259575 None:intergenic
CTTTGAGAAGCTTGAGTCGA+TGG 0.481104 5.2:-19259603 MS.gene028002:CDS
TGCTGACTATTACGCAGAAA+TGG 0.488469 5.2:-19259561 MS.gene028002:CDS
CTCCCCTTGATCAATATCAA+TGG 0.492911 5.2:+19259700 None:intergenic
CCGATCTCCTGGAGATACTC+CGG 0.494759 5.2:+19259247 None:intergenic
AGGAGATCGGTCAGGAGGTA+AGG 0.497659 5.2:-19259234 MS.gene028002:CDS
AGTCTTTGAGCTTTGAGGAT+GGG 0.501140 5.2:-19259301 MS.gene028002:CDS
AGTCAGCAGGTCTCAAGAAA+GGG 0.501463 5.2:+19259576 None:intergenic
GGGAGATAGCCACATGGAAA+AGG 0.502899 5.2:-19259534 MS.gene028002:CDS
CATAAGCTGGCCTGAGAATG+TGG 0.503617 5.2:-19259741 MS.gene028002:CDS
GTCACAGAAATTGAAAGAAA+GGG 0.504083 5.2:-19259417 MS.gene028002:CDS
GCAAAGCAAGCTATGGGAAA+GGG 0.507585 5.2:-19259212 MS.gene028002:CDS
ATTAGATGAGAAGCGGAAGA+TGG 0.510158 5.2:-19259498 MS.gene028002:CDS
GGCATTGGAGGGAACTAGAC+AGG 0.514311 5.2:-19259627 MS.gene028002:CDS
AGGTAAGGCAAAGCAAGCTA+TGG 0.517763 5.2:-19259219 MS.gene028002:CDS
TTTGAGAAGCTTGAGTCGAT+GGG 0.525398 5.2:-19259602 MS.gene028002:CDS
GGAGGCTGAAGAGAGAAGAA+AGG 0.530598 5.2:-19259474 MS.gene028002:CDS
CAAAGACTTATCTGGAGCAT+CGG 0.536868 5.2:+19259316 None:intergenic
AGATGAGAAGCGGAAGATGG+AGG 0.539024 5.2:-19259495 MS.gene028002:CDS
GATGCTATATTAGATAAACT+TGG 0.546950 5.2:-19259767 MS.gene028002:CDS
AGCAAAGAGATTGTCTAAAG+AGG 0.557828 5.2:-19259444 MS.gene028002:CDS
AAGAGGCCCGGAGTATCTCC+AGG 0.558097 5.2:-19259254 MS.gene028002:CDS
CTCCATTGATATTGATCAAG+GGG 0.559298 5.2:-19259702 MS.gene028002:CDS
GTATCTCCAGGAGATCGGTC+AGG 0.562327 5.2:-19259242 MS.gene028002:CDS
CGATCTCCTGGAGATACTCC+GGG 0.563080 5.2:+19259248 None:intergenic
AGTAGATGTGAACGATGACT+TGG 0.565329 5.2:-19259675 MS.gene028002:CDS
ATTTCTGCGTAATAGTCAGC+AGG 0.568461 5.2:+19259563 None:intergenic
ATAAACTTGGAGACATAAGC+TGG 0.571619 5.2:-19259754 MS.gene028002:CDS
TGAAACTGAAGATTTGGATG+AGG 0.572001 5.2:-19259867 MS.gene028002:CDS
CCGGAGTATCTCCAGGAGAT+CGG 0.576336 5.2:-19259247 MS.gene028002:CDS
AAGTCTTTGAGCTTTGAGGA+TGG 0.577328 5.2:-19259302 MS.gene028002:CDS
TGAAGAGAGAAGAAAGGCCA+GGG 0.579670 5.2:-19259468 MS.gene028002:CDS
CATCGACTCAAGCTTCTCAA+AGG 0.582387 5.2:+19259604 None:intergenic
GGCAAAGCAAGCTATGGGAA+AGG 0.584114 5.2:-19259213 MS.gene028002:CDS
ATTGGATCACCATTAATCAA+AGG 0.591452 5.2:+19259899 None:intergenic
TCTCCATTGATATTGATCAA+GGG 0.594778 5.2:-19259703 MS.gene028002:CDS
AATGGTGATCCAATGATTGA+CGG 0.595326 5.2:-19259890 MS.gene028002:CDS
GGTAAGGCAAAGCAAGCTAT+GGG 0.598074 5.2:-19259218 MS.gene028002:CDS
GGAAGCAGAGACAACAGAGT+GGG 0.599709 5.2:-19259352 MS.gene028002:CDS
ACATTGAATCTGTTAAGAAG+TGG 0.600860 5.2:-19259376 MS.gene028002:CDS
TGAGAAGCGGAAGATGGAGG+AGG 0.603783 5.2:-19259492 MS.gene028002:CDS
TTGAATCTGTTAAGAAGTGG+AGG 0.608378 5.2:-19259373 MS.gene028002:CDS
CTATTACGCAGAAATGGTGA+AGG 0.608653 5.2:-19259555 MS.gene028002:CDS
GTCGGCTATTAGATGAGAAG+CGG 0.609331 5.2:-19259505 MS.gene028002:CDS
GCTGGCCTGAGAATGTGGAT+TGG 0.611875 5.2:-19259736 MS.gene028002:CDS
ACATGGAAAAGGTGAAAAGT+CGG 0.619320 5.2:-19259523 MS.gene028002:CDS
CAACAGAGTGGGTTTGCTGA+CGG 0.625190 5.2:-19259341 MS.gene028002:CDS
AGGAAGCAGAGACAACAGAG+TGG 0.651328 5.2:-19259353 MS.gene028002:CDS
CATTGATATTGATCAAGGGG+AGG 0.657330 5.2:-19259699 MS.gene028002:CDS
TCTCCAGGAGATCGGTCAGG+AGG 0.664692 5.2:-19259239 MS.gene028002:CDS
TATTACGCAGAAATGGTGAA+GGG 0.681147 5.2:-19259554 MS.gene028002:CDS
GGTGAAGGGAGATAGCCACA+TGG 0.757517 5.2:-19259540 MS.gene028002:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATGCTATATTAGATAAACT+TGG - chr5.2:19259199-19259218 MS.gene028002:CDS 25.0%
!! TTAGTATCTCTTTTCTTTTG+CGG + chr5.2:19259787-19259806 None:intergenic 25.0%
ACATTGAATCTGTTAAGAAG+TGG - chr5.2:19259590-19259609 MS.gene028002:CDS 30.0%
AGTCACAGAAATTGAAAGAA+AGG - chr5.2:19259548-19259567 MS.gene028002:CDS 30.0%
ATTGGATCACCATTAATCAA+AGG + chr5.2:19259070-19259089 None:intergenic 30.0%
GATACTAAGAATGCCAAATT+TGG - chr5.2:19259799-19259818 MS.gene028002:CDS 30.0%
GTCACAGAAATTGAAAGAAA+GGG - chr5.2:19259549-19259568 MS.gene028002:CDS 30.0%
TCTCCATTGATATTGATCAA+GGG - chr5.2:19259263-19259282 MS.gene028002:CDS 30.0%
TCTTCTGAATCTGATTCAAA+TGG + chr5.2:19259133-19259152 None:intergenic 30.0%
! GAAACAACTAATGATTTTGG+CGG - chr5.2:19259862-19259881 MS.gene028002:CDS 30.0%
! GCTGAAACAACTAATGATTT+TGG - chr5.2:19259859-19259878 MS.gene028002:CDS 30.0%
!! AAACAACTAATGATTTTGGC+GGG - chr5.2:19259863-19259882 MS.gene028002:CDS 30.0%
!! AAGAATGCCAAATTTGGTTT+TGG - chr5.2:19259805-19259824 MS.gene028002:CDS 30.0%
!!! TTATTCACTGCAGTTTTAGA+TGG + chr5.2:19259175-19259194 None:intergenic 30.0%
AATGGTGATCCAATGATTGA+CGG - chr5.2:19259076-19259095 MS.gene028002:CDS 35.0%
ACATGGAAAAGGTGAAAAGT+CGG - chr5.2:19259443-19259462 MS.gene028002:CDS 35.0%
AGATAAGTCTTTGAGCTTTG+AGG - chr5.2:19259660-19259679 MS.gene028002:CDS 35.0%
AGCAAAGAGATTGTCTAAAG+AGG - chr5.2:19259522-19259541 MS.gene028002:CDS 35.0%
ATAAACTTGGAGACATAAGC+TGG - chr5.2:19259212-19259231 MS.gene028002:CDS 35.0%
CTCCATTGATATTGATCAAG+GGG - chr5.2:19259264-19259283 MS.gene028002:CDS 35.0%
CTCTCCATTGATATTGATCA+AGG - chr5.2:19259262-19259281 MS.gene028002:CDS 35.0%
TATTACGCAGAAATGGTGAA+GGG - chr5.2:19259412-19259431 MS.gene028002:CDS 35.0%
TCAAAGCTCAAAGACTTATC+TGG + chr5.2:19259661-19259680 None:intergenic 35.0%
TCGAGAGATCAAAGAAAAAG+AGG - chr5.2:19259695-19259714 MS.gene028002:CDS 35.0%
TGAAACTGAAGATTTGGATG+AGG - chr5.2:19259099-19259118 MS.gene028002:CDS 35.0%
TTGAATCTGTTAAGAAGTGG+AGG - chr5.2:19259593-19259612 MS.gene028002:CDS 35.0%
! AATGTCATCCTTTTTCTGCT+TGG + chr5.2:19259575-19259594 None:intergenic 35.0%
!!! AATGCCAAATTTGGTTTTGG+AGG - chr5.2:19259808-19259827 MS.gene028002:CDS 35.0%
AGATCAAAGAAAAAGAGGCC+CGG - chr5.2:19259700-19259719 MS.gene028002:CDS 40.0%
AGTAGATGTGAACGATGACT+TGG - chr5.2:19259291-19259310 MS.gene028002:CDS 40.0%
ATTAGATGAGAAGCGGAAGA+TGG - chr5.2:19259468-19259487 MS.gene028002:CDS 40.0%
ATTTCTGCGTAATAGTCAGC+AGG + chr5.2:19259406-19259425 None:intergenic 40.0%
CATTGATATTGATCAAGGGG+AGG - chr5.2:19259267-19259286 MS.gene028002:CDS 40.0%
CTATTACGCAGAAATGGTGA+AGG - chr5.2:19259411-19259430 MS.gene028002:CDS 40.0%
CTCCCCTTGATCAATATCAA+TGG + chr5.2:19259269-19259288 None:intergenic 40.0%
TCAGTTTCACCGTCAATCAT+TGG + chr5.2:19259088-19259107 None:intergenic 40.0%
TGACGGTGAAACTGAAGATT+TGG - chr5.2:19259093-19259112 MS.gene028002:CDS 40.0%
TTTGAGAAGCTTGAGTCGAT+GGG - chr5.2:19259364-19259383 MS.gene028002:CDS 40.0%
! ATCATTTTACACGCAGGCAT+TGG - chr5.2:19259324-19259343 MS.gene028002:CDS 40.0%
! CAAAGACTTATCTGGAGCAT+CGG + chr5.2:19259653-19259672 None:intergenic 40.0%
! GACTTTTCACCTTTTCCATG+TGG + chr5.2:19259444-19259463 None:intergenic 40.0%
! TGAGCTATCATTTTACACGC+AGG - chr5.2:19259318-19259337 MS.gene028002:CDS 40.0%
! TGCTGACTATTACGCAGAAA+TGG - chr5.2:19259405-19259424 MS.gene028002:CDS 40.0%
!! AAGTCTTTGAGCTTTGAGGA+TGG - chr5.2:19259664-19259683 MS.gene028002:CDS 40.0%
!! AGTCTTTGAGCTTTGAGGAT+GGG - chr5.2:19259665-19259684 MS.gene028002:CDS 40.0%
!! ATGGAGGTGCCTTTGATTAA+TGG - chr5.2:19259058-19259077 MS.gene028002:CDS 40.0%
AGAAAGGGCCAAGCAGAAAA+AGG - chr5.2:19259564-19259583 MS.gene028002:CDS 45.0%
AGACAATCTCTTTGCTTCCC+TGG + chr5.2:19259518-19259537 None:intergenic 45.0%
AGGTAAGGCAAAGCAAGCTA+TGG - chr5.2:19259747-19259766 MS.gene028002:CDS 45.0%
AGTCAGCAGGTCTCAAGAAA+GGG + chr5.2:19259393-19259412 None:intergenic 45.0%
GCAAAGCAAGCTATGGGAAA+GGG - chr5.2:19259754-19259773 MS.gene028002:CDS 45.0%
GGTAAGGCAAAGCAAGCTAT+GGG - chr5.2:19259748-19259767 MS.gene028002:CDS 45.0%
GTCGGCTATTAGATGAGAAG+CGG - chr5.2:19259461-19259480 MS.gene028002:CDS 45.0%
TAGTCAGCAGGTCTCAAGAA+AGG + chr5.2:19259394-19259413 None:intergenic 45.0%
TGAAGAGAGAAGAAAGGCCA+GGG - chr5.2:19259498-19259517 MS.gene028002:CDS 45.0%
TGCTTCCAATCCACATTCTC+AGG + chr5.2:19259238-19259257 None:intergenic 45.0%
! CTTTGAGAAGCTTGAGTCGA+TGG - chr5.2:19259363-19259382 MS.gene028002:CDS 45.0%
!! CATCGACTCAAGCTTCTCAA+AGG + chr5.2:19259365-19259384 None:intergenic 45.0%
!!! ATTTTACACGCAGGCATTGG+AGG - chr5.2:19259327-19259346 MS.gene028002:CDS 45.0%
!!! CCAAATTTGGTTTTGGAGGC+CGG - chr5.2:19259812-19259831 MS.gene028002:CDS 45.0%
!!! GATTTTGGCGGGTTCAGTAA+GGG - chr5.2:19259874-19259893 MS.gene028002:CDS 45.0%
!!! TGATTTTGGCGGGTTCAGTA+AGG - chr5.2:19259873-19259892 MS.gene028002:CDS 45.0%
!!! TTTTACACGCAGGCATTGGA+GGG - chr5.2:19259328-19259347 MS.gene028002:CDS 45.0%
AGATGAGAAGCGGAAGATGG+AGG - chr5.2:19259471-19259490 MS.gene028002:CDS 50.0%
AGGAAGCAGAGACAACAGAG+TGG - chr5.2:19259613-19259632 MS.gene028002:CDS 50.0%
CCGGCCTCCAAAACCAAATT+TGG + chr5.2:19259815-19259834 None:intergenic 50.0%
CTGAAGAGAGAAGAAAGGCC+AGG - chr5.2:19259497-19259516 MS.gene028002:CDS 50.0%
GGAAGCAGAGACAACAGAGT+GGG - chr5.2:19259614-19259633 MS.gene028002:CDS 50.0%
GGAGGCTGAAGAGAGAAGAA+AGG - chr5.2:19259492-19259511 MS.gene028002:CDS 50.0%
GGCAAAGCAAGCTATGGGAA+AGG - chr5.2:19259753-19259772 MS.gene028002:CDS 50.0%
GGGAGATAGCCACATGGAAA+AGG - chr5.2:19259432-19259451 MS.gene028002:CDS 50.0%
TCTGCTTCTTCGATCCCTTC+CGG + chr5.2:19259834-19259853 None:intergenic 50.0%
TTCAGTAAGGGTTCTGCTGC+TGG - chr5.2:19259886-19259905 MS.gene028002:CDS 50.0%
! CATAAGCTGGCCTGAGAATG+TGG - chr5.2:19259225-19259244 MS.gene028002:CDS 50.0%
!! CAACAGAGTGGGTTTGCTGA+CGG - chr5.2:19259625-19259644 MS.gene028002:CDS 50.0%
!!! ATTTGGTTTTGGAGGCCGGA+AGG - chr5.2:19259816-19259835 MS.gene028002:CDS 50.0%
!!! TTTGGTTTTGGAGGCCGGAA+GGG - chr5.2:19259817-19259836 MS.gene028002:CDS 50.0%
AGGAGATCGGTCAGGAGGTA+AGG - chr5.2:19259732-19259751 MS.gene028002:CDS 55.0%
CCGATCTCCTGGAGATACTC+CGG + chr5.2:19259722-19259741 None:intergenic 55.0%
CGATCTCCTGGAGATACTCC+GGG + chr5.2:19259721-19259740 None:intergenic 55.0%
GGTGAAGGGAGATAGCCACA+TGG - chr5.2:19259426-19259445 MS.gene028002:CDS 55.0%
GTATCTCCAGGAGATCGGTC+AGG - chr5.2:19259724-19259743 MS.gene028002:CDS 55.0%
TGAGAAGCGGAAGATGGAGG+AGG - chr5.2:19259474-19259493 MS.gene028002:CDS 55.0%
TTACCTCCTGACCGATCTCC+TGG + chr5.2:19259733-19259752 None:intergenic 55.0%
! CCGGAGTATCTCCAGGAGAT+CGG - chr5.2:19259719-19259738 MS.gene028002:CDS 55.0%
! GCTGGCCTGAGAATGTGGAT+TGG - chr5.2:19259230-19259249 MS.gene028002:CDS 55.0%
! GGCATTGGAGGGAACTAGAC+AGG - chr5.2:19259339-19259358 MS.gene028002:CDS 55.0%
TCTCCAGGAGATCGGTCAGG+AGG - chr5.2:19259727-19259746 MS.gene028002:CDS 60.0%
! AAGAGGCCCGGAGTATCTCC+AGG - chr5.2:19259712-19259731 MS.gene028002:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 19259058 19259930 19259058 ID=MS.gene028002
chr5.2 mRNA 19259058 19259930 19259058 ID=MS.gene028002.t1;Parent=MS.gene028002
chr5.2 exon 19259058 19259930 19259058 ID=MS.gene028002.t1.exon1;Parent=MS.gene028002.t1
chr5.2 CDS 19259058 19259930 19259058 ID=cds.MS.gene028002.t1;Parent=MS.gene028002.t1
Gene Sequence

>MS.gene028002

ATGGAGGTGCCTTTGATTAATGGTGATCCAATGATTGACGGTGAAACTGAAGATTTGGATGAGGAAATGTCACCATTTGAATCAGATTCAGAAGAAGATGTCAAATTAGCTGAACCATCTAAAACTGCAGTGAATAACAGAGATGCTATATTAGATAAACTTGGAGACATAAGCTGGCCTGAGAATGTGGATTGGAAGCACAAACTCTCCATTGATATTGATCAAGGGGAGGAAGTAGATGTGAACGATGACTTGGCACGTGAGCTATCATTTTACACGCAGGCATTGGAGGGAACTAGACAGGCCTTTGAGAAGCTTGAGTCGATGGGTCTCCCTTTCTTGAGACCTGCTGACTATTACGCAGAAATGGTGAAGGGAGATAGCCACATGGAAAAGGTGAAAAGTCGGCTATTAGATGAGAAGCGGAAGATGGAGGAGGCTGAAGAGAGAAGAAAGGCCAGGGAAGCAAAGAGATTGTCTAAAGAGGTTCAGTCACAGAAATTGAAAGAAAGGGCCAAGCAGAAAAAGGATGACATTGAATCTGTTAAGAAGTGGAGGAAGCAGAGACAACAGAGTGGGTTTGCTGACGGCGCCGATGCTCCAGATAAGTCTTTGAGCTTTGAGGATGGGAAAGTGTTCGAGAGATCAAAGAAAAAGAGGCCCGGAGTATCTCCAGGAGATCGGTCAGGAGGTAAGGCAAAGCAAGCTATGGGAAAGGGAAAGAAGCCGCAAAAGAAAAGAGATACTAAGAATGCCAAATTTGGTTTTGGAGGCCGGAAGGGATCGAAGAAGCAGAATACAGCTGAAACAACTAATGATTTTGGCGGGTTCAGTAAGGGTTCTGCTGCTGGAAATAAGAAGAGAAAGAGATAA

Protein sequence

>MS.gene028002.t1

MEVPLINGDPMIDGETEDLDEEMSPFESDSEEDVKLAEPSKTAVNNRDAILDKLGDISWPENVDWKHKLSIDIDQGEEVDVNDDLARELSFYTQALEGTRQAFEKLESMGLPFLRPADYYAEMVKGDSHMEKVKSRLLDEKRKMEEAEERRKAREAKRLSKEVQSQKLKERAKQKKDDIESVKKWRKQRQQSGFADGADAPDKSLSFEDGKVFERSKKKRPGVSPGDRSGGKAKQAMGKGKKPQKKRDTKNAKFGFGGRKGSKKQNTAETTNDFGGFSKGSAAGNKKRKR