AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58854


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58854.t1 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene58854.t1 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.67e-66 204
MS.gene58854.t1 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.15e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58854.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT5G59690 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT3G46320 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT3G45930 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT3G53730 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT2G28740 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT1G07660 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene58854.t1 AT1G07660 83.495 103 0 1 1 103 1 86 4.34e-51 155

Find 0 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGATCTATGAGGAAACAAG+AGG + 47696:3990-4009 MS.gene58854:CDS 35.0%
AATCAGCGGTTTGATCTATG+AGG + 47696:3980-3999 MS.gene58854:CDS 40.0%
GGAGGTAAAGGATTAGGAAA+GGG + 47696:3861-3880 MS.gene58854:CDS 40.0%
GTTTATGCCCTTAAGAGACA+GGG + 47696:4104-4123 MS.gene58854:CDS 40.0%
TGATTCCTTGAATGTTGTCG+CGG - 47696:3914-3933 MS.gene58854:intergenic 40.0%
TGTTTATGCCCTTAAGAGAC+AGG + 47696:4103-4122 MS.gene58854:CDS 40.0%
!! AGGAGTGTTGAAGATCTTCT+TGG + 47696:4010-4029 MS.gene58854:CDS 40.0%
AAAGCCAGCAATTCGTCGAT+TGG + 47696:3935-3954 MS.gene58854:CDS 45.0%
GGAAAGGGAGGTAAAGGATT+AGG + 47696:3855-3874 MS.gene58854:CDS 45.0%
GGAAGAACCCTCTATGGTTT+CGG + 47696:4125-4144 MS.gene58854:CDS 45.0%
GGGAGGTAAAGGATTAGGAA+AGG + 47696:3860-3879 MS.gene58854:CDS 45.0%
GGTAAAGGATTAGGAAAGGG+AGG + 47696:3864-3883 MS.gene58854:CDS 45.0%
GGTTCTTCCCTGTCTCTTAA+GGG - 47696:4114-4133 MS.gene58854:intergenic 45.0%
TCAACCACCGAAACCATAGA+GGG - 47696:4135-4154 MS.gene58854:intergenic 45.0%
TTACTTACACTGAGCATGCG+AGG + 47696:4054-4073 MS.gene58854:CDS 45.0%
! GGAGGTGTGAAAAGAATCAG+CGG + 47696:3966-3985 MS.gene58854:CDS 45.0%
AGAACCCTCTATGGTTTCGG+TGG + 47696:4128-4147 MS.gene58854:CDS 50.0%
AGACAGGGAAGAACCCTCTA+TGG + 47696:4119-4138 MS.gene58854:CDS 50.0%
AGGTGCAAAGAGACATCGCA+AGG + 47696:3884-3903 MS.gene58854:CDS 50.0%
GAGGAAGACTGTGACTGCTA+TGG + 47696:4076-4095 MS.gene58854:CDS 50.0%
GGGTTCTTCCCTGTCTCTTA+AGG - 47696:4115-4134 MS.gene58854:intergenic 50.0%
GTTCTCCGCGACAACATTCA+AGG + 47696:3906-3925 MS.gene58854:CDS 50.0%
! ATTCGTCGATTGGCGAGAAG+AGG + 47696:3945-3964 MS.gene58854:CDS 50.0%
ATGTCTGGTCGTGGAAAGGG+AGG + 47696:3843-3862 MS.gene58854:CDS 55.0%
CTCGCCAATCGACGAATTGC+TGG - 47696:3942-3961 MS.gene58854:intergenic 55.0%
CTTACACTGAGCATGCGAGG+AGG + 47696:4057-4076 MS.gene58854:CDS 55.0%
GGTCGTGGAAAGGGAGGTAA+AGG + 47696:3849-3868 MS.gene58854:CDS 55.0%
! CGTCGATTGGCGAGAAGAGG+AGG + 47696:3948-3967 MS.gene58854:CDS 60.0%


Chromosome Type Strat End Strand Name
47696 gene 3843 4154 3843 ID=MS.gene58854
47696 mRNA 3843 4154 3843 ID=MS.gene58854.t1;Parent=MS.gene58854
47696 exon 3843 4154 3843 ID=MS.gene58854.t1.exon1;Parent=MS.gene58854.t1
47696 CDS 3843 4154 3843 ID=cds.MS.gene58854.t1;Parent=MS.gene58854.t1
Gene Sequence

>MS.gene58854

ATGTCTGGTCGTGGAAAGGGAGGTAAAGGATTAGGAAAGGGAGGTGCAAAGAGACATCGCAAGGTTCTCCGCGACAACATTCAAGGAATCACAAAGCCAGCAATTCGTCGATTGGCGAGAAGAGGAGGTGTGAAAAGAATCAGCGGTTTGATCTATGAGGAAACAAGAGGAGTGTTGAAGATCTTCTTGGAGAATGTGATAAGAGATGCTGTTACTTACACTGAGCATGCGAGGAGGAAGACTGTGACTGCTATGGATGTTGTTTATGCCCTTAAGAGACAGGGAAGAACCCTCTATGGTTTCGGTGGTTGA

Protein sequence

>MS.gene58854.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG