Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072942.t1 | XP_003603460.2 | 97 | 203 | 0 | 1 | 1 | 197 | 695 | 897 | 2.70E-110 | 407.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072942.t1 | Q9FUY2 | 74.3 | 206 | 43 | 2 | 2 | 197 | 726 | 931 | 4.2e-88 | 325.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072942.t1 | A0A072V282 | 97.0 | 203 | 0 | 1 | 1 | 197 | 702 | 904 | 1.9e-110 | 407.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene072942 | 0.846155 | 2.59E-59 | -1.69E-46 |
| MS.gene049254 | MS.gene072942 | 0.832638 | 8.43E-56 | -1.69E-46 |
| MS.gene049272 | MS.gene072942 | 0.825103 | 5.62E-54 | -1.69E-46 |
| MS.gene049343 | MS.gene072942 | 0.840159 | 1.03E-57 | -1.69E-46 |
| MS.gene049404 | MS.gene072942 | 0.801516 | 8.68E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene072942 | 0.812487 | 4.14E-51 | -1.69E-46 |
| MS.gene049452 | MS.gene072942 | 0.832975 | 6.96E-56 | -1.69E-46 |
| MS.gene049669 | MS.gene072942 | 0.806078 | 9.79E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene072942 | 0.83761 | 4.70E-57 | -1.69E-46 |
| MS.gene049850 | MS.gene072942 | 0.832293 | 1.03E-55 | -1.69E-46 |
| MS.gene049873 | MS.gene072942 | 0.842628 | 2.30E-58 | -1.69E-46 |
| MS.gene050026 | MS.gene072942 | 0.830183 | 3.39E-55 | -1.69E-46 |
| MS.gene050156 | MS.gene072942 | 0.815096 | 1.10E-51 | -1.69E-46 |
| MS.gene050201 | MS.gene072942 | 0.801916 | 7.18E-49 | -1.69E-46 |
| MS.gene050355 | MS.gene072942 | 0.848144 | 7.39E-60 | -1.69E-46 |
| MS.gene050442 | MS.gene072942 | 0.824335 | 8.54E-54 | -1.69E-46 |
| MS.gene050799 | MS.gene072942 | 0.827398 | 1.60E-54 | -1.69E-46 |
| MS.gene050844 | MS.gene072942 | 0.811354 | 7.30E-51 | -1.69E-46 |
| MS.gene050905 | MS.gene072942 | -0.837676 | 4.52E-57 | -1.69E-46 |
| MS.gene050953 | MS.gene072942 | 0.801853 | 7.40E-49 | -1.69E-46 |
| MS.gene051181 | MS.gene072942 | 0.813385 | 2.63E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene072942 | 0.808699 | 2.72E-50 | -1.69E-46 |
| MS.gene051363 | MS.gene072942 | 0.806623 | 7.52E-50 | -1.69E-46 |
| MS.gene051368 | MS.gene072942 | 0.81655 | 5.22E-52 | -1.69E-46 |
| MS.gene051594 | MS.gene072942 | 0.815796 | 7.69E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene072942 | 0.828131 | 1.07E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene072942 | 0.810384 | 1.18E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene072942 | 0.807682 | 4.49E-50 | -1.69E-46 |
| MS.gene051924 | MS.gene072942 | -0.815723 | 7.99E-52 | -1.69E-46 |
| MS.gene051926 | MS.gene072942 | -0.816634 | 5.00E-52 | -1.69E-46 |
| MS.gene052012 | MS.gene072942 | 0.800581 | 1.35E-48 | -1.69E-46 |
| MS.gene052190 | MS.gene072942 | 0.823642 | 1.24E-53 | -1.69E-46 |
| MS.gene052208 | MS.gene072942 | 0.809389 | 1.94E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene072942 | 0.827916 | 1.20E-54 | -1.69E-46 |
| MS.gene052358 | MS.gene072942 | -0.827705 | 1.35E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene072942 | 0.890252 | 1.17E-73 | -1.69E-46 |
| MS.gene052582 | MS.gene072942 | 0.854164 | 1.48E-61 | -1.69E-46 |
| MS.gene052584 | MS.gene072942 | 0.857492 | 1.57E-62 | -1.69E-46 |
| MS.gene052586 | MS.gene072942 | 0.814361 | 1.60E-51 | -1.69E-46 |
| MS.gene052611 | MS.gene072942 | 0.803585 | 3.25E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene072942 | 0.847417 | 1.17E-59 | -1.69E-46 |
| MS.gene052863 | MS.gene072942 | 0.862045 | 6.69E-64 | -1.69E-46 |
| MS.gene052867 | MS.gene072942 | 0.809672 | 1.69E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene072942 | 0.816224 | 6.17E-52 | -1.69E-46 |
| MS.gene052965 | MS.gene072942 | -0.802967 | 4.36E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene072942 | 0.817713 | 2.86E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene072942 | 0.839734 | 1.33E-57 | -1.69E-46 |
| MS.gene05308 | MS.gene072942 | 0.802653 | 5.07E-49 | -1.69E-46 |
| MS.gene053145 | MS.gene072942 | 0.822311 | 2.54E-53 | -1.69E-46 |
| MS.gene053166 | MS.gene072942 | 0.841139 | 5.69E-58 | -1.69E-46 |
| MS.gene053202 | MS.gene072942 | 0.816782 | 4.63E-52 | -1.69E-46 |
| MS.gene053221 | MS.gene072942 | 0.803111 | 4.07E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene072942 | 0.851223 | 1.02E-60 | -1.69E-46 |
| MS.gene05350 | MS.gene072942 | 0.804438 | 2.16E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene072942 | 0.804099 | 2.54E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene072942 | 0.826348 | 2.85E-54 | -1.69E-46 |
| MS.gene05415 | MS.gene072942 | 0.830488 | 2.86E-55 | -1.69E-46 |
| MS.gene054236 | MS.gene072942 | 0.841911 | 3.56E-58 | -1.69E-46 |
| MS.gene05423 | MS.gene072942 | 0.817026 | 4.08E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene072942 | -0.852369 | 4.83E-61 | -1.69E-46 |
| MS.gene054467 | MS.gene072942 | 0.810207 | 1.29E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene072942 | 0.825785 | 3.88E-54 | -1.69E-46 |
| MS.gene054479 | MS.gene072942 | 0.803862 | 2.85E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene072942 | 0.845139 | 4.89E-59 | -1.69E-46 |
| MS.gene054582 | MS.gene072942 | 0.800968 | 1.12E-48 | -1.69E-46 |
| MS.gene05458 | MS.gene072942 | 0.820305 | 7.36E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene072942 | 0.854607 | 1.10E-61 | -1.69E-46 |
| MS.gene054657 | MS.gene072942 | 0.810161 | 1.32E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene072942 | 0.838408 | 2.93E-57 | -1.69E-46 |
| MS.gene054994 | MS.gene072942 | 0.811812 | 5.81E-51 | -1.69E-46 |
| MS.gene055166 | MS.gene072942 | 0.830915 | 2.24E-55 | -1.69E-46 |
| MS.gene05517 | MS.gene072942 | 0.812886 | 3.38E-51 | -1.69E-46 |
| MS.gene055227 | MS.gene072942 | 0.815483 | 9.03E-52 | -1.69E-46 |
| MS.gene055228 | MS.gene072942 | 0.814719 | 1.34E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene072942 | 0.800496 | 1.40E-48 | -1.69E-46 |
| MS.gene055512 | MS.gene072942 | 0.806968 | 6.36E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene072942 | 0.815824 | 7.58E-52 | -1.69E-46 |
| MS.gene055589 | MS.gene072942 | 0.81522 | 1.03E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene072942 | 0.81639 | 5.67E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene072942 | 0.82085 | 5.52E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene072942 | 0.863945 | 1.73E-64 | -1.69E-46 |
| MS.gene055841 | MS.gene072942 | 0.812378 | 4.37E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene072942 | 0.811162 | 8.04E-51 | -1.69E-46 |
| MS.gene05604 | MS.gene072942 | 0.812992 | 3.21E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene072942 | 0.805353 | 1.39E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene072942 | 0.811029 | 8.59E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene072942 | 0.82025 | 7.58E-53 | -1.69E-46 |
| MS.gene056498 | MS.gene072942 | 0.806684 | 7.30E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene072942 | 0.837767 | 4.28E-57 | -1.69E-46 |
| MS.gene056595 | MS.gene072942 | 0.822194 | 2.70E-53 | -1.69E-46 |
| MS.gene056642 | MS.gene072942 | 0.81429 | 1.66E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene072942 | 0.802322 | 5.93E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene072942 | 0.849943 | 2.34E-60 | -1.69E-46 |
| MS.gene057060 | MS.gene072942 | 0.824261 | 8.88E-54 | -1.69E-46 |
| MS.gene057208 | MS.gene072942 | 0.857302 | 1.79E-62 | -1.69E-46 |
| MS.gene057414 | MS.gene072942 | 0.804235 | 2.38E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene072942 | 0.819351 | 1.22E-52 | -1.69E-46 |
| MS.gene057598 | MS.gene072942 | 0.835971 | 1.23E-56 | -1.69E-46 |
| MS.gene057693 | MS.gene072942 | 0.840897 | 6.59E-58 | -1.69E-46 |
| MS.gene057855 | MS.gene072942 | 0.803243 | 3.83E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene072942 | 0.820074 | 8.31E-53 | -1.69E-46 |
| MS.gene058008 | MS.gene072942 | 0.801081 | 1.07E-48 | -1.69E-46 |
| MS.gene058009 | MS.gene072942 | 0.819278 | 1.26E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene072942 | 0.850935 | 1.23E-60 | -1.69E-46 |
| MS.gene058548 | MS.gene072942 | 0.809615 | 1.73E-50 | -1.69E-46 |
| MS.gene058549 | MS.gene072942 | 0.807414 | 5.12E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene072942 | 0.870447 | 1.45E-66 | -1.69E-46 |
| MS.gene058636 | MS.gene072942 | 0.805595 | 1.24E-49 | -1.69E-46 |
| MS.gene058783 | MS.gene072942 | 0.815423 | 9.32E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene072942 | 0.809471 | 1.86E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene072942 | 0.857207 | 1.91E-62 | -1.69E-46 |
| MS.gene059272 | MS.gene072942 | 0.810106 | 1.36E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene072942 | 0.855229 | 7.26E-62 | -1.69E-46 |
| MS.gene059584 | MS.gene072942 | 0.80003 | 1.74E-48 | -1.69E-46 |
| MS.gene059625 | MS.gene072942 | 0.809251 | 2.08E-50 | -1.69E-46 |
| MS.gene05979 | MS.gene072942 | 0.826607 | 2.47E-54 | -1.69E-46 |
| MS.gene059842 | MS.gene072942 | 0.833753 | 4.45E-56 | -1.69E-46 |
| MS.gene060137 | MS.gene072942 | -0.812162 | 4.87E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene072942 | 0.854542 | 1.15E-61 | -1.69E-46 |
| MS.gene060521 | MS.gene072942 | 0.82542 | 4.73E-54 | -1.69E-46 |
| MS.gene06052 | MS.gene072942 | 0.813233 | 2.84E-51 | -1.69E-46 |
| MS.gene060553 | MS.gene072942 | 0.800281 | 1.55E-48 | -1.69E-46 |
| MS.gene060572 | MS.gene072942 | 0.829453 | 5.10E-55 | -1.69E-46 |
| MS.gene060634 | MS.gene072942 | -0.831826 | 1.34E-55 | -1.69E-46 |
| MS.gene060685 | MS.gene072942 | 0.804273 | 2.34E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene072942 | 0.833273 | 5.86E-56 | -1.69E-46 |
| MS.gene060879 | MS.gene072942 | 0.804712 | 1.89E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene072942 | 0.808818 | 2.57E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene072942 | -0.857587 | 1.47E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072942.t1 | MTR_3g107910 | 97.044 | 203 | 0 | 1 | 1 | 197 | 702 | 904 | 5.45e-141 | 415 |
| MS.gene072942.t1 | MTR_3g107910 | 97.044 | 203 | 0 | 1 | 1 | 197 | 695 | 897 | 6.98e-141 | 415 |
| MS.gene072942.t1 | MTR_1g011610 | 85.990 | 207 | 19 | 2 | 1 | 197 | 687 | 893 | 1.83e-125 | 375 |
| MS.gene072942.t1 | MTR_1g011610 | 85.990 | 207 | 19 | 2 | 1 | 197 | 696 | 902 | 2.83e-125 | 374 |
| MS.gene072942.t1 | MTR_1g050340 | 57.353 | 204 | 80 | 2 | 1 | 197 | 584 | 787 | 3.07e-80 | 254 |
| MS.gene072942.t1 | MTR_1g050340 | 57.353 | 204 | 80 | 2 | 1 | 197 | 583 | 786 | 3.73e-80 | 254 |
| MS.gene072942.t1 | MTR_7g058440 | 52.451 | 204 | 90 | 2 | 1 | 197 | 572 | 775 | 2.64e-73 | 236 |
| MS.gene072942.t1 | MTR_7g058460 | 52.451 | 204 | 90 | 2 | 1 | 197 | 581 | 784 | 3.45e-73 | 235 |
| MS.gene072942.t1 | MTR_4g113080 | 52.195 | 205 | 87 | 4 | 2 | 197 | 579 | 781 | 7.66e-67 | 218 |
| MS.gene072942.t1 | MTR_2g014020 | 52.683 | 205 | 87 | 4 | 1 | 197 | 322 | 524 | 2.06e-66 | 212 |
| MS.gene072942.t1 | MTR_4g088620 | 38.350 | 206 | 117 | 4 | 1 | 197 | 104 | 308 | 1.17e-43 | 148 |
| MS.gene072942.t1 | MTR_4g088635 | 38.462 | 208 | 115 | 6 | 1 | 197 | 178 | 383 | 5.59e-43 | 148 |
| MS.gene072942.t1 | MTR_4g088625 | 39.320 | 206 | 113 | 6 | 1 | 197 | 97 | 299 | 2.23e-41 | 142 |
| MS.gene072942.t1 | MTR_4g088640 | 38.350 | 206 | 115 | 6 | 1 | 197 | 181 | 383 | 1.23e-38 | 136 |
| MS.gene072942.t1 | MTR_4g088660 | 36.318 | 201 | 118 | 5 | 5 | 197 | 283 | 481 | 1.11e-36 | 133 |
| MS.gene072942.t1 | MTR_4g088665 | 33.673 | 196 | 116 | 5 | 15 | 197 | 188 | 382 | 4.64e-30 | 114 |
| MS.gene072942.t1 | MTR_4g088670 | 34.595 | 185 | 105 | 4 | 10 | 188 | 112 | 286 | 1.56e-27 | 105 |
| MS.gene072942.t1 | MTR_2g014020 | 54.945 | 91 | 40 | 1 | 1 | 91 | 322 | 411 | 1.21e-26 | 105 |
| MS.gene072942.t1 | MTR_1g035350 | 45.631 | 103 | 17 | 3 | 101 | 197 | 25 | 94 | 1.58e-19 | 79.7 |
| MS.gene072942.t1 | MTR_4g088675 | 30.303 | 198 | 87 | 7 | 11 | 197 | 63 | 220 | 1.40e-18 | 80.5 |
| MS.gene072942.t1 | MTR_5g022690 | 26.442 | 208 | 139 | 6 | 2 | 196 | 269 | 475 | 8.51e-17 | 78.2 |
| MS.gene072942.t1 | MTR_2g012630 | 24.155 | 207 | 145 | 5 | 2 | 196 | 5 | 211 | 3.26e-13 | 67.8 |
| MS.gene072942.t1 | MTR_6g004040 | 30.921 | 152 | 97 | 6 | 5 | 150 | 94 | 243 | 6.80e-12 | 63.9 |
| MS.gene072942.t1 | MTR_6g004040 | 25.517 | 145 | 100 | 6 | 7 | 145 | 54 | 196 | 1.54e-11 | 62.8 |
| MS.gene072942.t1 | MTR_4g119620 | 27.404 | 208 | 134 | 8 | 5 | 196 | 71 | 277 | 8.99e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072942.t1 | AT4G32551 | 74.272 | 206 | 43 | 2 | 2 | 197 | 726 | 931 | 1.32e-107 | 329 |
| MS.gene072942.t1 | AT4G32551 | 74.272 | 206 | 43 | 2 | 2 | 197 | 764 | 969 | 3.67e-107 | 328 |
| MS.gene072942.t1 | AT2G32700 | 57.073 | 205 | 79 | 4 | 1 | 197 | 584 | 787 | 7.06e-80 | 253 |
| MS.gene072942.t1 | AT2G32700 | 57.073 | 205 | 79 | 4 | 1 | 197 | 584 | 787 | 7.06e-80 | 253 |
| MS.gene072942.t1 | AT2G32700 | 57.073 | 205 | 79 | 4 | 1 | 197 | 584 | 787 | 7.06e-80 | 253 |
| MS.gene072942.t1 | AT2G32700 | 57.073 | 205 | 79 | 4 | 1 | 197 | 584 | 787 | 7.06e-80 | 253 |
| MS.gene072942.t1 | AT2G32700 | 57.073 | 205 | 79 | 4 | 1 | 197 | 584 | 787 | 7.06e-80 | 253 |
| MS.gene072942.t1 | AT2G32700 | 57.073 | 205 | 79 | 4 | 1 | 197 | 582 | 785 | 8.17e-80 | 253 |
| MS.gene072942.t1 | AT2G32700 | 57.073 | 205 | 79 | 4 | 1 | 197 | 603 | 806 | 1.24e-79 | 253 |
| MS.gene072942.t1 | AT5G23430 | 33.871 | 124 | 75 | 5 | 5 | 123 | 94 | 215 | 1.44e-11 | 63.2 |
| MS.gene072942.t1 | AT5G23430 | 33.871 | 124 | 75 | 5 | 5 | 123 | 94 | 215 | 1.44e-11 | 63.2 |
| MS.gene072942.t1 | AT5G23430 | 33.871 | 124 | 75 | 5 | 5 | 123 | 94 | 215 | 1.55e-11 | 63.2 |
Find 44 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATATAAGATCATCTTTATT+TGG | 0.065820 | 3.1:-85434503 | None:intergenic |
| TTGGCGGTTTCAACTGTAAA+TGG | 0.301887 | 3.1:+85435399 | MS.gene072942:CDS |
| ATATAAGATCATCTTTATTT+GGG | 0.338718 | 3.1:-85434502 | None:intergenic |
| GTGAGGACTCTGTCAGGATT+TGG | 0.366793 | 3.1:+85435079 | MS.gene072942:CDS |
| GGCATGCCGATATTCATTAA+AGG | 0.372801 | 3.1:+85434839 | MS.gene072942:CDS |
| GGATCCATCGGGAGAATTGC+TGG | 0.381986 | 3.1:+85435047 | MS.gene072942:CDS |
| GACGAGGTTGAAATCTCATC+TGG | 0.387712 | 3.1:-85434755 | None:intergenic |
| GACAATAGATTCTGTATGTT+GGG | 0.395558 | 3.1:+85435026 | MS.gene072942:CDS |
| CAGTTGTGTTAGAGTCTCTA+AGG | 0.417904 | 3.1:+85434574 | MS.gene072942:CDS |
| TGATGGTTTAATTACTGCCT+TGG | 0.438872 | 3.1:+85435380 | MS.gene072942:CDS |
| ACGACAAGTTCATCAAGCTT+TGG | 0.441767 | 3.1:+85435442 | MS.gene072942:CDS |
| GTCAGGATTTGGACTCTTGA+AGG | 0.442934 | 3.1:+85435090 | MS.gene072942:CDS |
| TGGTTTAATTACTGCCTTGG+CGG | 0.462707 | 3.1:+85435383 | MS.gene072942:CDS |
| TTCTGTATGTTGGGATCCAT+CGG | 0.462819 | 3.1:+85435035 | MS.gene072942:CDS |
| AGACAATAGATTCTGTATGT+TGG | 0.466596 | 3.1:+85435025 | MS.gene072942:CDS |
| CTGTGAGTGAGGACTCTGTC+AGG | 0.473083 | 3.1:+85435073 | MS.gene072942:CDS |
| CTTGATGAACTTGTCGTGAC+TGG | 0.476097 | 3.1:-85435436 | None:intergenic |
| GTTCATGAGCTTAGCTGTAA+TGG | 0.482292 | 3.1:+85435120 | MS.gene072942:CDS |
| TCAGGATTTGGACTCTTGAA+GGG | 0.495748 | 3.1:+85435091 | MS.gene072942:CDS |
| ATGACTTTGTCTGCTCATGA+TGG | 0.507469 | 3.1:+85435363 | MS.gene072942:CDS |
| CAACCAATGACGAGCAGTGA+AGG | 0.518554 | 3.1:-85435180 | None:intergenic |
| ATGAGATTTCAACCTCGTCT+AGG | 0.533516 | 3.1:+85434759 | MS.gene072942:CDS |
| CATCGTGTCTATACTTGATG+TGG | 0.541488 | 3.1:+85434809 | MS.gene072942:CDS |
| TGAGATTTCAACCTCGTCTA+GGG | 0.547964 | 3.1:+85434760 | MS.gene072942:CDS |
| ACGAGCAGTGAAGGGAATGT+TGG | 0.559129 | 3.1:-85435171 | None:intergenic |
| AACCAATGACGAGCAGTGAA+GGG | 0.566783 | 3.1:-85435179 | None:intergenic |
| GTCATTGGAGCTGTGGAACA+TGG | 0.570092 | 3.1:+85435326 | MS.gene072942:intron |
| TCTGTATGTTGGGATCCATC+GGG | 0.588620 | 3.1:+85435036 | MS.gene072942:CDS |
| CAGGATTTGGACTCTTGAAG+GGG | 0.592600 | 3.1:+85435092 | MS.gene072942:CDS |
| GCAGTGAAGGGAATGTTGGA+TGG | 0.593172 | 3.1:-85435167 | None:intergenic |
| ATATGTTCGTGTGATGGTGA+TGG | 0.610859 | 3.1:+85434521 | MS.gene072942:CDS |
| GATGCCAGCAATTCTCCCGA+TGG | 0.613289 | 3.1:-85435051 | None:intergenic |
| GATCTTATATGTTCGTGTGA+TGG | 0.621408 | 3.1:+85434515 | MS.gene072942:CDS |
| ACGAGGTTGAAATCTCATCT+GGG | 0.623795 | 3.1:-85434754 | None:intergenic |
| TTGTGTAGTCATTGGAGCTG+TGG | 0.626523 | 3.1:+85435319 | MS.gene072942:intron |
| GTGATGGTGAGATACGGTAT+TGG | 0.634594 | 3.1:+85434537 | MS.gene072942:CDS |
| TATACTTGATGTGGAAACAC+AGG | 0.635213 | 3.1:+85434818 | MS.gene072942:CDS |
| ATTGCTGGCATCTGTGAGTG+AGG | 0.640756 | 3.1:+85435062 | MS.gene072942:CDS |
| GTGATGGTGATGGTGAGATA+CGG | 0.649915 | 3.1:+85434531 | MS.gene072942:CDS |
| GCAAGGTACCTCCCTAGACG+AGG | 0.669924 | 3.1:-85434771 | None:intergenic |
| ATTTACAGTTGAAACCGCCA+AGG | 0.674075 | 3.1:-85435397 | None:intergenic |
| GATTTCAACCTCGTCTAGGG+AGG | 0.679700 | 3.1:+85434763 | MS.gene072942:CDS |
| CGGTATTGGAGCATAAACAA+CGG | 0.729220 | 3.1:+85434551 | MS.gene072942:CDS |
| AAAGTGCGAAGAGAATACCC+AGG | 0.782358 | 3.1:-85434440 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATAAGATCATCTTTATTT+GGG | - | chr3.1:85434505-85434524 | None:intergenic | 15.0% |
| !!! | CTTTATCTTCTATTTTTTAA+GGG | + | chr3.1:85434670-85434689 | MS.gene072942:intron | 15.0% |
| !! | CATATAAGATCATCTTTATT+TGG | - | chr3.1:85434506-85434525 | None:intergenic | 20.0% |
| !! | CCTTAAAAAATAGAAGATAA+AGG | - | chr3.1:85434672-85434691 | None:intergenic | 20.0% |
| !! | GAACTTAAAATATTAACCAA+TGG | + | chr3.1:85434964-85434983 | MS.gene072942:intron | 20.0% |
| !!! | CCTTTATCTTCTATTTTTTA+AGG | + | chr3.1:85434669-85434688 | MS.gene072942:intron | 20.0% |
| !!! | GTTTTACCTTTAATGAATAT+CGG | - | chr3.1:85434848-85434867 | None:intergenic | 20.0% |
| ! | AAGAAATTCATGCTTGATAT+TGG | + | chr3.1:85435290-85435309 | MS.gene072942:intron | 25.0% |
| ! | AATGTTGTACAATGATTGTA+GGG | + | chr3.1:85434994-85435013 | MS.gene072942:intron | 25.0% |
| ! | CTTAATTGCATATTAGAGTA+GGG | - | chr3.1:85435273-85435292 | None:intergenic | 25.0% |
| ! | TAATGTTGTACAATGATTGT+AGG | + | chr3.1:85434993-85435012 | MS.gene072942:intron | 25.0% |
| ! | TACAACATTAAAACAACCAT+TGG | - | chr3.1:85434983-85435002 | None:intergenic | 25.0% |
| ! | TCTTAATTGCATATTAGAGT+AGG | - | chr3.1:85435274-85435293 | None:intergenic | 25.0% |
| !! | TAAAGTGACAATTTTCTTGT+TGG | + | chr3.1:85434717-85434736 | MS.gene072942:intron | 25.0% |
| !! | TCTAAAACTATGATGATAAC+CGG | - | chr3.1:85434941-85434960 | None:intergenic | 25.0% |
| AGACAATAGATTCTGTATGT+TGG | + | chr3.1:85435025-85435044 | MS.gene072942:CDS | 30.0% | |
| CTTGTTGATATTTGCGAATT+TGG | + | chr3.1:85435231-85435250 | MS.gene072942:intron | 30.0% | |
| GACAATAGATTCTGTATGTT+GGG | + | chr3.1:85435026-85435045 | MS.gene072942:CDS | 30.0% | |
| TAGAAGTTCTACTTGAGTTA+GGG | + | chr3.1:85434887-85434906 | MS.gene072942:intron | 30.0% | |
| TCAACAAGAAAATAAAGACG+TGG | - | chr3.1:85435219-85435238 | None:intergenic | 30.0% | |
| TTAGAAGTTCTACTTGAGTT+AGG | + | chr3.1:85434886-85434905 | MS.gene072942:intron | 30.0% | |
| ! | AAGATAAAAAGTGCACAACT+CGG | - | chr3.1:85434916-85434935 | None:intergenic | 30.0% |
| !! | GACAATTTTCTTGTTGGTTA+GGG | + | chr3.1:85434723-85434742 | MS.gene072942:intron | 30.0% |
| !! | TGACAATTTTCTTGTTGGTT+AGG | + | chr3.1:85434722-85434741 | MS.gene072942:intron | 30.0% |
| !! | TGTGCACTTTTTATCTTATC+CGG | + | chr3.1:85434919-85434938 | MS.gene072942:intron | 30.0% |
| ATAGAAGATAAAGGCCATCT+AGG | - | chr3.1:85434663-85434682 | None:intergenic | 35.0% | |
| GATCTTATATGTTCGTGTGA+TGG | + | chr3.1:85434515-85434534 | MS.gene072942:CDS | 35.0% | |
| TAATACGCAAGCTACAAAAC+AGG | - | chr3.1:85434699-85434718 | None:intergenic | 35.0% | |
| TAGAAGATAAAGGCCATCTA+GGG | - | chr3.1:85434662-85434681 | None:intergenic | 35.0% | |
| TATACTTGATGTGGAAACAC+AGG | + | chr3.1:85434818-85434837 | MS.gene072942:CDS | 35.0% | |
| TGATGGTTTAATTACTGCCT+TGG | + | chr3.1:85435380-85435399 | MS.gene072942:CDS | 35.0% | |
| ! | GTATTCTCTTCGCACTTTTA+CGG | + | chr3.1:85434445-85434464 | MS.gene072942:CDS | 35.0% |
| ! | TATTCTCTTCGCACTTTTAC+GGG | + | chr3.1:85434446-85434465 | MS.gene072942:CDS | 35.0% |
| ! | TGCAAAGCATCTTGTACAAA+GGG | - | chr3.1:85434638-85434657 | None:intergenic | 35.0% |
| !! | AATTTTCTTGTTGGTTAGGG+TGG | + | chr3.1:85434726-85434745 | MS.gene072942:intron | 35.0% |
| ACGACAAGTTCATCAAGCTT+TGG | + | chr3.1:85435442-85435461 | MS.gene072942:CDS | 40.0% | |
| ACGAGGTTGAAATCTCATCT+GGG | - | chr3.1:85434757-85434776 | None:intergenic | 40.0% | |
| ATGACTTTGTCTGCTCATGA+TGG | + | chr3.1:85435363-85435382 | MS.gene072942:CDS | 40.0% | |
| ATGAGATTTCAACCTCGTCT+AGG | + | chr3.1:85434759-85434778 | MS.gene072942:CDS | 40.0% | |
| ATTTACAGTTGAAACCGCCA+AGG | - | chr3.1:85435400-85435419 | None:intergenic | 40.0% | |
| CAGTTGTGTTAGAGTCTCTA+AGG | + | chr3.1:85434574-85434593 | MS.gene072942:CDS | 40.0% | |
| CATCGTGTCTATACTTGATG+TGG | + | chr3.1:85434809-85434828 | MS.gene072942:CDS | 40.0% | |
| GGCATGCCGATATTCATTAA+AGG | + | chr3.1:85434839-85434858 | MS.gene072942:CDS | 40.0% | |
| GGTACATGTTGTGTAGTCAT+TGG | + | chr3.1:85435311-85435330 | MS.gene072942:intron | 40.0% | |
| GTTCATGAGCTTAGCTGTAA+TGG | + | chr3.1:85435120-85435139 | MS.gene072942:CDS | 40.0% | |
| TCAGGATTTGGACTCTTGAA+GGG | + | chr3.1:85435091-85435110 | MS.gene072942:CDS | 40.0% | |
| TGAGATTTCAACCTCGTCTA+GGG | + | chr3.1:85434760-85434779 | MS.gene072942:CDS | 40.0% | |
| TGGTTTAATTACTGCCTTGG+CGG | + | chr3.1:85435383-85435402 | MS.gene072942:CDS | 40.0% | |
| TTCTGTATGTTGGGATCCAT+CGG | + | chr3.1:85435035-85435054 | MS.gene072942:CDS | 40.0% | |
| TTGGCGGTTTCAACTGTAAA+TGG | + | chr3.1:85435399-85435418 | MS.gene072942:CDS | 40.0% | |
| ! | ATATGTTCGTGTGATGGTGA+TGG | + | chr3.1:85434521-85434540 | MS.gene072942:CDS | 40.0% |
| ! | CGGTATTGGAGCATAAACAA+CGG | + | chr3.1:85434551-85434570 | MS.gene072942:CDS | 40.0% |
| ! | CTGCAAAGCATCTTGTACAA+AGG | - | chr3.1:85434639-85434658 | None:intergenic | 40.0% |
| ! | GCAAAGCATCTTGTACAAAG+GGG | - | chr3.1:85434637-85434656 | None:intergenic | 40.0% |
| AAAGTGCGAAGAGAATACCC+AGG | - | chr3.1:85434443-85434462 | None:intergenic | 45.0% | |
| AACCAATGACGAGCAGTGAA+GGG | - | chr3.1:85435182-85435201 | None:intergenic | 45.0% | |
| AATAAAGACGTGGACTTGCC+TGG | - | chr3.1:85435209-85435228 | None:intergenic | 45.0% | |
| CAGGATTTGGACTCTTGAAG+GGG | + | chr3.1:85435092-85435111 | MS.gene072942:CDS | 45.0% | |
| CTTGATGAACTTGTCGTGAC+TGG | - | chr3.1:85435439-85435458 | None:intergenic | 45.0% | |
| GACGAGGTTGAAATCTCATC+TGG | - | chr3.1:85434758-85434777 | None:intergenic | 45.0% | |
| GTCAGGATTTGGACTCTTGA+AGG | + | chr3.1:85435090-85435109 | MS.gene072942:CDS | 45.0% | |
| TCTGTATGTTGGGATCCATC+GGG | + | chr3.1:85435036-85435055 | MS.gene072942:CDS | 45.0% | |
| TTGTGTAGTCATTGGAGCTG+TGG | + | chr3.1:85435319-85435338 | MS.gene072942:intron | 45.0% | |
| ! | GTGATGGTGAGATACGGTAT+TGG | + | chr3.1:85434537-85434556 | MS.gene072942:CDS | 45.0% |
| !! | GTGATGGTGATGGTGAGATA+CGG | + | chr3.1:85434531-85434550 | MS.gene072942:CDS | 45.0% |
| ACGAGCAGTGAAGGGAATGT+TGG | - | chr3.1:85435174-85435193 | None:intergenic | 50.0% | |
| AGATGCTTTGCAGCCCTAGA+TGG | + | chr3.1:85434646-85434665 | MS.gene072942:intron | 50.0% | |
| ATTGCTGGCATCTGTGAGTG+AGG | + | chr3.1:85435062-85435081 | MS.gene072942:CDS | 50.0% | |
| CAACCAATGACGAGCAGTGA+AGG | - | chr3.1:85435183-85435202 | None:intergenic | 50.0% | |
| GATTTCAACCTCGTCTAGGG+AGG | + | chr3.1:85434763-85434782 | MS.gene072942:CDS | 50.0% | |
| GCAGTGAAGGGAATGTTGGA+TGG | - | chr3.1:85435170-85435189 | None:intergenic | 50.0% | |
| GTCATTGGAGCTGTGGAACA+TGG | + | chr3.1:85435326-85435345 | MS.gene072942:intron | 50.0% | |
| GTGAGGACTCTGTCAGGATT+TGG | + | chr3.1:85435079-85435098 | MS.gene072942:CDS | 50.0% | |
| TTCCCTTCACTGCTCGTCAT+TGG | + | chr3.1:85435177-85435196 | MS.gene072942:intron | 50.0% | |
| ! | GCTCGTCATTGGTTGTTACC+AGG | + | chr3.1:85435188-85435207 | MS.gene072942:intron | 50.0% |
| ! | TGTTTTCAGCTGCTGCAGCA+AGG | - | chr3.1:85434791-85434810 | None:intergenic | 50.0% |
| CTGTGAGTGAGGACTCTGTC+AGG | + | chr3.1:85435073-85435092 | MS.gene072942:CDS | 55.0% | |
| GATGCCAGCAATTCTCCCGA+TGG | - | chr3.1:85435054-85435073 | None:intergenic | 55.0% | |
| GGATCCATCGGGAGAATTGC+TGG | + | chr3.1:85435047-85435066 | MS.gene072942:CDS | 55.0% | |
| ! | GCAAGGTACCTCCCTAGACG+AGG | - | chr3.1:85434774-85434793 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 85434440 | 85435470 | 85434440 | ID=MS.gene072942 |
| chr3.1 | mRNA | 85434440 | 85435470 | 85434440 | ID=MS.gene072942.t1;Parent=MS.gene072942 |
| chr3.1 | exon | 85434440 | 85434595 | 85434440 | ID=MS.gene072942.t1.exon1;Parent=MS.gene072942.t1 |
| chr3.1 | CDS | 85434440 | 85434595 | 85434440 | ID=cds.MS.gene072942.t1;Parent=MS.gene072942.t1 |
| chr3.1 | exon | 85434744 | 85434860 | 85434744 | ID=MS.gene072942.t1.exon2;Parent=MS.gene072942.t1 |
| chr3.1 | CDS | 85434744 | 85434860 | 85434744 | ID=cds.MS.gene072942.t1;Parent=MS.gene072942.t1 |
| chr3.1 | exon | 85435015 | 85435191 | 85435015 | ID=MS.gene072942.t1.exon3;Parent=MS.gene072942.t1 |
| chr3.1 | CDS | 85435015 | 85435191 | 85435015 | ID=cds.MS.gene072942.t1;Parent=MS.gene072942.t1 |
| chr3.1 | exon | 85435327 | 85435470 | 85435327 | ID=MS.gene072942.t1.exon4;Parent=MS.gene072942.t1 |
| chr3.1 | CDS | 85435327 | 85435470 | 85435327 | ID=cds.MS.gene072942.t1;Parent=MS.gene072942.t1 |
| Gene Sequence |
| Protein sequence |
