Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21928.t1 | XP_003610935.1 | 97.5 | 122 | 3 | 0 | 111 | 232 | 381 | 502 | 7.50E-64 | 253.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21928.t1 | Q93ZG9 | 76.7 | 120 | 27 | 1 | 112 | 230 | 358 | 477 | 1.5e-49 | 197.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21928.t1 | B7FLX2 | 97.5 | 122 | 3 | 0 | 111 | 232 | 381 | 502 | 5.4e-64 | 253.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene21928 | 0.803068 | 4.16E-49 | -1.69E-46 |
| MS.gene049207 | MS.gene21928 | 0.806364 | 8.53E-50 | -1.69E-46 |
| MS.gene049255 | MS.gene21928 | 0.810769 | 9.78E-51 | -1.69E-46 |
| MS.gene049343 | MS.gene21928 | 0.805741 | 1.15E-49 | -1.69E-46 |
| MS.gene049473 | MS.gene21928 | 0.818686 | 1.72E-52 | -1.69E-46 |
| MS.gene049666 | MS.gene21928 | 0.80594 | 1.05E-49 | -1.69E-46 |
| MS.gene049824 | MS.gene21928 | 0.83306 | 6.62E-56 | -1.69E-46 |
| MS.gene049825 | MS.gene21928 | 0.866218 | 3.35E-65 | -1.69E-46 |
| MS.gene049873 | MS.gene21928 | 0.863828 | 1.88E-64 | -1.69E-46 |
| MS.gene050277 | MS.gene21928 | 0.830381 | 3.03E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene21928 | 0.887465 | 1.39E-72 | -1.69E-46 |
| MS.gene050355 | MS.gene21928 | 0.83147 | 1.64E-55 | -1.69E-46 |
| MS.gene050719 | MS.gene21928 | 0.800739 | 1.25E-48 | -1.69E-46 |
| MS.gene050721 | MS.gene21928 | 0.804613 | 1.99E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene21928 | 0.801675 | 8.05E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene21928 | 0.816302 | 5.93E-52 | -1.69E-46 |
| MS.gene05134 | MS.gene21928 | 0.829272 | 5.65E-55 | -1.69E-46 |
| MS.gene051451 | MS.gene21928 | 0.800197 | 1.61E-48 | -1.69E-46 |
| MS.gene051452 | MS.gene21928 | 0.802676 | 5.01E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene21928 | 0.831615 | 1.51E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene21928 | 0.820273 | 7.48E-53 | -1.69E-46 |
| MS.gene05188 | MS.gene21928 | 0.851937 | 6.41E-61 | -1.69E-46 |
| MS.gene051975 | MS.gene21928 | 0.842336 | 2.75E-58 | -1.69E-46 |
| MS.gene052012 | MS.gene21928 | 0.815731 | 7.96E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene21928 | 0.842629 | 2.30E-58 | -1.69E-46 |
| MS.gene052582 | MS.gene21928 | 0.824501 | 7.80E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene21928 | 0.837199 | 5.98E-57 | -1.69E-46 |
| MS.gene052947 | MS.gene21928 | 0.822516 | 2.27E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene21928 | 0.839021 | 2.03E-57 | -1.69E-46 |
| MS.gene053008 | MS.gene21928 | 0.825035 | 5.84E-54 | -1.69E-46 |
| MS.gene053058 | MS.gene21928 | 0.800697 | 1.28E-48 | -1.69E-46 |
| MS.gene053080 | MS.gene21928 | 0.834977 | 2.19E-56 | -1.69E-46 |
| MS.gene053226 | MS.gene21928 | 0.869256 | 3.55E-66 | -1.69E-46 |
| MS.gene05365 | MS.gene21928 | 0.837245 | 5.82E-57 | -1.69E-46 |
| MS.gene053995 | MS.gene21928 | 0.818035 | 2.42E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene21928 | 0.828045 | 1.12E-54 | -1.69E-46 |
| MS.gene054657 | MS.gene21928 | 0.812178 | 4.83E-51 | -1.69E-46 |
| MS.gene054658 | MS.gene21928 | 0.816151 | 6.41E-52 | -1.69E-46 |
| MS.gene055166 | MS.gene21928 | 0.819629 | 1.05E-52 | -1.69E-46 |
| MS.gene05517 | MS.gene21928 | 0.827634 | 1.40E-54 | -1.69E-46 |
| MS.gene055222 | MS.gene21928 | 0.807287 | 5.44E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene21928 | 0.813644 | 2.31E-51 | -1.69E-46 |
| MS.gene055555 | MS.gene21928 | 0.829068 | 6.33E-55 | -1.69E-46 |
| MS.gene055586 | MS.gene21928 | 0.820797 | 5.67E-53 | -1.69E-46 |
| MS.gene055589 | MS.gene21928 | 0.830663 | 2.59E-55 | -1.69E-46 |
| MS.gene055603 | MS.gene21928 | 0.814385 | 1.58E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene21928 | 0.802641 | 5.09E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene21928 | 0.85337 | 2.50E-61 | -1.69E-46 |
| MS.gene055808 | MS.gene21928 | 0.876744 | 1.09E-68 | -1.69E-46 |
| MS.gene055818 | MS.gene21928 | 0.812343 | 4.45E-51 | -1.69E-46 |
| MS.gene055841 | MS.gene21928 | 0.80727 | 5.49E-50 | -1.69E-46 |
| MS.gene056091 | MS.gene21928 | 0.871335 | 7.39E-67 | -1.69E-46 |
| MS.gene056215 | MS.gene21928 | 0.824929 | 6.18E-54 | -1.69E-46 |
| MS.gene056403 | MS.gene21928 | 0.851716 | 7.40E-61 | -1.69E-46 |
| MS.gene056506 | MS.gene21928 | 0.802614 | 5.16E-49 | -1.69E-46 |
| MS.gene05659 | MS.gene21928 | 0.811292 | 7.53E-51 | -1.69E-46 |
| MS.gene056605 | MS.gene21928 | 0.854715 | 1.02E-61 | -1.69E-46 |
| MS.gene056631 | MS.gene21928 | 0.860928 | 1.47E-63 | -1.69E-46 |
| MS.gene056642 | MS.gene21928 | 0.802074 | 6.67E-49 | -1.69E-46 |
| MS.gene05689 | MS.gene21928 | 0.837074 | 6.44E-57 | -1.69E-46 |
| MS.gene056986 | MS.gene21928 | 0.855359 | 6.65E-62 | -1.69E-46 |
| MS.gene057060 | MS.gene21928 | 0.804648 | 1.95E-49 | -1.69E-46 |
| MS.gene057079 | MS.gene21928 | 0.813816 | 2.11E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene21928 | 0.800594 | 1.34E-48 | -1.69E-46 |
| MS.gene057140 | MS.gene21928 | 0.817198 | 3.73E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene21928 | 0.801156 | 1.03E-48 | -1.69E-46 |
| MS.gene057314 | MS.gene21928 | 0.811745 | 6.00E-51 | -1.69E-46 |
| MS.gene057555 | MS.gene21928 | 0.815445 | 9.21E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene21928 | 0.832655 | 8.35E-56 | -1.69E-46 |
| MS.gene057705 | MS.gene21928 | 0.826739 | 2.30E-54 | -1.69E-46 |
| MS.gene05795 | MS.gene21928 | 0.804252 | 2.36E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene21928 | 0.843428 | 1.41E-58 | -1.69E-46 |
| MS.gene058084 | MS.gene21928 | 0.835631 | 1.50E-56 | -1.69E-46 |
| MS.gene058126 | MS.gene21928 | 0.808958 | 2.40E-50 | -1.69E-46 |
| MS.gene058238 | MS.gene21928 | 0.808168 | 3.54E-50 | -1.69E-46 |
| MS.gene058498 | MS.gene21928 | 0.814337 | 1.62E-51 | -1.69E-46 |
| MS.gene058500 | MS.gene21928 | 0.806392 | 8.41E-50 | -1.69E-46 |
| MS.gene058548 | MS.gene21928 | 0.804842 | 1.78E-49 | -1.69E-46 |
| MS.gene058549 | MS.gene21928 | 0.820672 | 6.06E-53 | -1.69E-46 |
| MS.gene058625 | MS.gene21928 | 0.809755 | 1.62E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene21928 | 0.803386 | 3.57E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene21928 | 0.828951 | 6.76E-55 | -1.69E-46 |
| MS.gene058911 | MS.gene21928 | 0.829182 | 5.94E-55 | -1.69E-46 |
| MS.gene059151 | MS.gene21928 | 0.804901 | 1.73E-49 | -1.69E-46 |
| MS.gene059249 | MS.gene21928 | 0.818902 | 1.54E-52 | -1.69E-46 |
| MS.gene05950 | MS.gene21928 | 0.816291 | 5.97E-52 | -1.69E-46 |
| MS.gene05953 | MS.gene21928 | 0.832181 | 1.09E-55 | -1.69E-46 |
| MS.gene05954 | MS.gene21928 | 0.880991 | 3.48E-70 | -1.69E-46 |
| MS.gene05979 | MS.gene21928 | 0.814348 | 1.61E-51 | -1.69E-46 |
| MS.gene059830 | MS.gene21928 | 0.854479 | 1.20E-61 | -1.69E-46 |
| MS.gene059842 | MS.gene21928 | 0.814417 | 1.56E-51 | -1.69E-46 |
| MS.gene059935 | MS.gene21928 | 0.884076 | 2.61E-71 | -1.69E-46 |
| MS.gene06001 | MS.gene21928 | 0.841871 | 3.65E-58 | -1.69E-46 |
| MS.gene06011 | MS.gene21928 | 0.825244 | 5.21E-54 | -1.69E-46 |
| MS.gene060365 | MS.gene21928 | 0.816076 | 6.66E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene21928 | 0.894858 | 1.66E-75 | -1.69E-46 |
| MS.gene060521 | MS.gene21928 | 0.813307 | 2.74E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene21928 | 0.821835 | 3.27E-53 | -1.69E-46 |
| MS.gene060586 | MS.gene21928 | 0.862375 | 5.30E-64 | -1.69E-46 |
| MS.gene060763 | MS.gene21928 | 0.819223 | 1.30E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene21928 | 0.805683 | 1.19E-49 | -1.69E-46 |
| MS.gene060830 | MS.gene21928 | 0.871951 | 4.62E-67 | -1.69E-46 |
| MS.gene060907 | MS.gene21928 | 0.823206 | 1.57E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene21928 | -0.817979 | 2.49E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene21928 | MS.gene002226 | PPI |
| MS.gene21928 | MS.gene02185 | PPI |
| MS.gene21928 | MS.gene02186 | PPI |
| MS.gene21928 | MS.gene01201 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21928.t1 | MTR_5g008680 | 95.322 | 171 | 7 | 1 | 62 | 232 | 333 | 502 | 8.89e-104 | 309 |
| MS.gene21928.t1 | MTR_5g008680 | 62.295 | 61 | 21 | 1 | 5 | 63 | 215 | 275 | 3.28e-12 | 65.9 |
| MS.gene21928.t1 | MTR_4g032680 | 56.667 | 120 | 51 | 1 | 112 | 230 | 642 | 761 | 7.83e-41 | 148 |
| MS.gene21928.t1 | MTR_4g032680 | 56.667 | 120 | 51 | 1 | 112 | 230 | 751 | 870 | 8.36e-41 | 148 |
| MS.gene21928.t1 | MTR_4g032680 | 56.667 | 120 | 51 | 1 | 112 | 230 | 751 | 870 | 8.36e-41 | 148 |
| MS.gene21928.t1 | MTR_4g032680 | 56.667 | 120 | 51 | 1 | 112 | 230 | 542 | 661 | 8.65e-41 | 147 |
| MS.gene21928.t1 | MTR_4g094785 | 35.714 | 182 | 91 | 7 | 59 | 227 | 69 | 237 | 1.64e-23 | 95.1 |
| MS.gene21928.t1 | MTR_8g012380 | 52.688 | 93 | 41 | 3 | 139 | 229 | 46 | 137 | 4.56e-21 | 86.3 |
| MS.gene21928.t1 | MTR_8g091410 | 32.973 | 185 | 103 | 7 | 59 | 229 | 29 | 206 | 1.10e-18 | 81.6 |
| MS.gene21928.t1 | MTR_1g061850 | 38.211 | 123 | 63 | 4 | 116 | 229 | 125 | 243 | 1.81e-17 | 79.0 |
| MS.gene21928.t1 | MTR_8g012340 | 48.315 | 89 | 43 | 3 | 141 | 227 | 56 | 143 | 8.21e-17 | 79.3 |
| MS.gene21928.t1 | MTR_8g080980 | 49.438 | 89 | 42 | 3 | 141 | 227 | 54 | 141 | 1.04e-16 | 79.0 |
| MS.gene21928.t1 | MTR_7g053470 | 47.126 | 87 | 43 | 3 | 143 | 227 | 52 | 137 | 1.14e-16 | 79.0 |
| MS.gene21928.t1 | MTR_8g038785 | 46.316 | 95 | 48 | 2 | 136 | 227 | 181 | 275 | 1.31e-16 | 78.6 |
| MS.gene21928.t1 | MTR_5g080300 | 40.659 | 91 | 48 | 3 | 146 | 232 | 35 | 123 | 1.05e-13 | 67.4 |
| MS.gene21928.t1 | MTR_4g068190 | 38.406 | 138 | 67 | 6 | 101 | 231 | 97 | 223 | 1.19e-13 | 68.2 |
| MS.gene21928.t1 | MTR_1g061080 | 36.667 | 120 | 43 | 3 | 143 | 229 | 96 | 215 | 1.49e-12 | 65.1 |
| MS.gene21928.t1 | MTR_8g006755 | 29.310 | 116 | 80 | 1 | 115 | 230 | 341 | 454 | 6.96e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21928.t1 | AT4G25340 | 68.919 | 148 | 32 | 3 | 84 | 230 | 343 | 477 | 3.47e-63 | 204 |
| MS.gene21928.t1 | AT5G05420 | 64.486 | 107 | 38 | 0 | 123 | 229 | 36 | 142 | 4.08e-47 | 153 |
| MS.gene21928.t1 | AT4G25340 | 66.667 | 105 | 22 | 2 | 84 | 188 | 343 | 434 | 1.46e-38 | 139 |
| MS.gene21928.t1 | AT3G12340 | 51.562 | 128 | 61 | 1 | 103 | 229 | 250 | 377 | 1.70e-37 | 135 |
| MS.gene21928.t1 | AT3G12340 | 51.562 | 128 | 61 | 1 | 103 | 229 | 250 | 377 | 1.70e-37 | 135 |
| MS.gene21928.t1 | AT3G12340 | 51.562 | 128 | 61 | 1 | 103 | 229 | 371 | 498 | 2.31e-36 | 134 |
| MS.gene21928.t1 | AT3G25220 | 51.546 | 97 | 40 | 4 | 139 | 231 | 48 | 141 | 4.57e-21 | 86.3 |
| MS.gene21928.t1 | AT5G48580 | 49.462 | 93 | 44 | 3 | 139 | 229 | 48 | 139 | 2.39e-20 | 84.7 |
| MS.gene21928.t1 | AT5G45680 | 39.695 | 131 | 63 | 5 | 111 | 228 | 79 | 206 | 3.04e-20 | 85.5 |
| MS.gene21928.t1 | AT3G25230 | 50.575 | 87 | 40 | 3 | 143 | 227 | 57 | 142 | 3.74e-18 | 83.2 |
| MS.gene21928.t1 | AT3G25230 | 50.575 | 87 | 40 | 3 | 143 | 227 | 57 | 142 | 4.93e-18 | 82.8 |
| MS.gene21928.t1 | AT5G48570 | 50.000 | 90 | 38 | 4 | 143 | 228 | 65 | 151 | 6.39e-18 | 82.4 |
| MS.gene21928.t1 | AT3G10060 | 35.256 | 156 | 82 | 6 | 85 | 229 | 69 | 216 | 1.48e-16 | 76.3 |
| MS.gene21928.t1 | AT4G39710 | 38.983 | 118 | 56 | 5 | 123 | 227 | 94 | 208 | 1.95e-15 | 72.8 |
| MS.gene21928.t1 | AT4G39710 | 38.983 | 118 | 56 | 5 | 123 | 227 | 94 | 208 | 1.95e-15 | 72.8 |
| MS.gene21928.t1 | AT3G55520 | 41.758 | 91 | 47 | 3 | 146 | 232 | 35 | 123 | 1.42e-14 | 70.1 |
| MS.gene21928.t1 | AT3G55520 | 41.758 | 91 | 47 | 3 | 146 | 232 | 35 | 123 | 1.42e-14 | 70.1 |
| MS.gene21928.t1 | AT3G55520 | 41.758 | 91 | 47 | 3 | 146 | 232 | 35 | 123 | 1.42e-14 | 70.1 |
| MS.gene21928.t1 | AT2G43560 | 38.261 | 115 | 62 | 4 | 123 | 231 | 106 | 217 | 1.93e-14 | 70.5 |
| MS.gene21928.t1 | AT2G43560 | 36.620 | 142 | 72 | 6 | 98 | 231 | 30 | 161 | 4.16e-14 | 68.2 |
Find 48 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTCTCCTTCAGCCTCTT+TGG | 0.225392 | 5.1:-2714112 | None:intergenic |
| TTTCTTCAGGGATGAGGGTT+GGG | 0.234866 | 5.1:+2714738 | MS.gene21928:intron |
| ACTTACAATTCCACCATCTA+TGG | 0.251042 | 5.1:+2714772 | MS.gene21928:CDS |
| CATACCAAGTCGAAACTTAA+AGG | 0.287309 | 5.1:-2714507 | None:intergenic |
| AAAATATTTGACTCGTGTAT+TGG | 0.338581 | 5.1:+2714476 | MS.gene21928:CDS |
| GGATAGGTTCTCACGTTAGA+TGG | 0.341955 | 5.1:-2714222 | None:intergenic |
| GGTTATAGAGGACATATCTA+TGG | 0.345544 | 5.1:+2714254 | MS.gene21928:CDS |
| GGAGTATACCACAAAATTCA+TGG | 0.385779 | 5.1:+2714958 | MS.gene21928:CDS |
| GCTTTGTCTGAAGTGGAAAT+CGG | 0.394739 | 5.1:-2712932 | None:intergenic |
| ACAATTTCATACCCCATAGA+TGG | 0.396140 | 5.1:-2714785 | None:intergenic |
| GTTGAGTTGGTTGGCGTTGA+AGG | 0.403304 | 5.1:+2714993 | MS.gene21928:CDS |
| GGGTGGGATGTTGGCGTCAA+TGG | 0.405209 | 5.1:+2714630 | MS.gene21928:CDS |
| TCCTCTATAACCAATCCATT+TGG | 0.417967 | 5.1:-2714243 | None:intergenic |
| AGATTTAGCTTTGTCTGAAG+TGG | 0.441957 | 5.1:-2712939 | None:intergenic |
| TTCTTCAGGGATGAGGGTTG+GGG | 0.443024 | 5.1:+2714739 | MS.gene21928:intron |
| GGTTCTCACGTTAGATGGCT+TGG | 0.444994 | 5.1:-2714217 | None:intergenic |
| GATGGCAAAAGAGCTGATCT+TGG | 0.455967 | 5.1:+2714285 | MS.gene21928:CDS |
| CAGGTCAGTGTTAGATATAC+CGG | 0.457744 | 5.1:+2714434 | MS.gene21928:intron |
| ATACCAAGTCGAAACTTAAA+GGG | 0.462033 | 5.1:-2714506 | None:intergenic |
| TCCAAATGGATTGGTTATAG+AGG | 0.465197 | 5.1:+2714242 | MS.gene21928:CDS |
| ATATTTGATGTTGAGTTGGT+TGG | 0.480042 | 5.1:+2714984 | MS.gene21928:CDS |
| CTTACAATTCCACCATCTAT+GGG | 0.490011 | 5.1:+2714773 | MS.gene21928:CDS |
| GTCATCAAGGGGTGGGATGT+TGG | 0.490964 | 5.1:+2714621 | MS.gene21928:CDS |
| GCTTATATTTGATGTTGAGT+TGG | 0.494265 | 5.1:+2714980 | MS.gene21928:CDS |
| AACGTGAGAACCTATCCAAA+TGG | 0.495137 | 5.1:+2714228 | MS.gene21928:CDS |
| AAGAGCTGATCTTGGAAAGA+AGG | 0.496054 | 5.1:+2714293 | MS.gene21928:CDS |
| CTTTGTCTGAAGTGGAAATC+GGG | 0.496076 | 5.1:-2712931 | None:intergenic |
| TTAGATGGCTTGGCTTCAGT+TGG | 0.504936 | 5.1:-2714207 | None:intergenic |
| TATAACCAATCCATTTGGAT+AGG | 0.528390 | 5.1:-2714238 | None:intergenic |
| AACTGGGAAATCCAAGAAGA+AGG | 0.539493 | 5.1:+2713013 | MS.gene21928:CDS |
| GCACCCTTTAAGTTTCGACT+TGG | 0.542842 | 5.1:+2714503 | MS.gene21928:CDS |
| AAGGCTGAAACAGAAACAAA+AGG | 0.552836 | 5.1:+2712971 | MS.gene21928:CDS |
| GTTATAGAGGACATATCTAT+GGG | 0.558134 | 5.1:+2714255 | MS.gene21928:CDS |
| GAGAACCTATCCAAATGGAT+TGG | 0.560765 | 5.1:+2714233 | MS.gene21928:CDS |
| AGGATGAGCAGCTGCAAGAT+GGG | 0.567860 | 5.1:+2713090 | MS.gene21928:CDS |
| AGGTGTAGGACAAGTCATCA+AGG | 0.569087 | 5.1:+2714608 | MS.gene21928:intron |
| AGCTAAATCTGAGTCTCAGA+AGG | 0.587477 | 5.1:+2712952 | MS.gene21928:CDS |
| GATATGGTGACAAACGTGTT+GGG | 0.590168 | 5.1:+2714937 | MS.gene21928:intron |
| ATATCTATGGGCAAACCAGA+TGG | 0.595038 | 5.1:+2714267 | MS.gene21928:CDS |
| GGTGTAGGACAAGTCATCAA+GGG | 0.596781 | 5.1:+2714609 | MS.gene21928:intron |
| GAGGATGAGCAGCTGCAAGA+TGG | 0.601018 | 5.1:+2713089 | MS.gene21928:CDS |
| AGGACAAGTCATCAAGGGGT+GGG | 0.609520 | 5.1:+2714614 | MS.gene21928:CDS |
| TAGGACAAGTCATCAAGGGG+TGG | 0.614183 | 5.1:+2714613 | MS.gene21928:CDS |
| ATTTCATACCCCATAGATGG+TGG | 0.621077 | 5.1:-2714782 | None:intergenic |
| AGATATGGTGACAAACGTGT+TGG | 0.627092 | 5.1:+2714936 | MS.gene21928:intron |
| AAAAGTTCAAGCTGTCGATG+AGG | 0.663434 | 5.1:+2713070 | MS.gene21928:CDS |
| GTGTAGGACAAGTCATCAAG+GGG | 0.668593 | 5.1:+2714610 | MS.gene21928:CDS |
| TTACAATTCCACCATCTATG+GGG | 0.701878 | 5.1:+2714774 | MS.gene21928:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATCTTTTATTTTCTTC+AGG | + | chr5.1:2714725-2714744 | MS.gene21928:intron | 15.0% |
| !!! | CAATTTTTAAAAAACAGATT+TGG | - | chr5.1:2713650-2713669 | None:intergenic | 15.0% |
| !!! | TTTATCTTTTATTTTCTTCA+GGG | + | chr5.1:2714726-2714745 | MS.gene21928:intron | 15.0% |
| !!! | TTTGAAAATTCAAAAACTTT+TGG | + | chr5.1:2713370-2713389 | MS.gene21928:intron | 15.0% |
| !! | AAGAAACTGGATTATAAAAT+AGG | - | chr5.1:2714388-2714407 | None:intergenic | 20.0% |
| !! | AATAAATAATCCAGATACTT+TGG | + | chr5.1:2713204-2713223 | MS.gene21928:intron | 20.0% |
| !! | AATCTTATTACGTTATTACT+AGG | - | chr5.1:2714670-2714689 | None:intergenic | 20.0% |
| !! | AGAATTAAAAGACAAACTAT+TGG | - | chr5.1:2713623-2713642 | None:intergenic | 20.0% |
| !! | ATAAATAATCCAGATACTTT+GGG | + | chr5.1:2713205-2713224 | MS.gene21928:intron | 20.0% |
| !! | CAAACGATAAATATAGATAA+AGG | - | chr5.1:2714587-2714606 | None:intergenic | 20.0% |
| !! | CAATATTGTACATTTCTAAA+TGG | - | chr5.1:2713537-2713556 | None:intergenic | 20.0% |
| !!! | AAAATTCAAAAACTTTTGGT+AGG | + | chr5.1:2713374-2713393 | MS.gene21928:intron | 20.0% |
| !!! | AAATTCAAAAAACTTTTGCT+AGG | + | chr5.1:2713276-2713295 | MS.gene21928:intron | 20.0% |
| !!! | TTTGGTACATTTTTCATTAA+TGG | + | chr5.1:2713493-2713512 | MS.gene21928:intron | 20.0% |
| ! | ACGTTTCTGATAATAATTTC+AGG | + | chr5.1:2713763-2713782 | MS.gene21928:intron | 25.0% |
| ! | ATCCCATTTAACAAAGAATA+TGG | - | chr5.1:2714874-2714893 | None:intergenic | 25.0% |
| ! | ATGCCATATTCTTTGTTAAA+TGG | + | chr5.1:2714868-2714887 | MS.gene21928:intron | 25.0% |
| ! | TATCTATATTTATCGTTTGC+AGG | + | chr5.1:2714588-2714607 | MS.gene21928:intron | 25.0% |
| ! | TGCCATATTCTTTGTTAAAT+GGG | + | chr5.1:2714869-2714888 | MS.gene21928:intron | 25.0% |
| ! | TTTCTATTCCTAAATCAAAC+AGG | - | chr5.1:2713177-2713196 | None:intergenic | 25.0% |
| !! | AAAAACAAGTTGAAAGATCA+TGG | + | chr5.1:2713801-2713820 | MS.gene21928:intron | 25.0% |
| !! | AAAACTGAAACAGATTTTAG+TGG | + | chr5.1:2713454-2713473 | MS.gene21928:intron | 25.0% |
| !! | AAAATATTTGACTCGTGTAT+TGG | + | chr5.1:2714476-2714495 | MS.gene21928:CDS | 25.0% |
| !! | AATATAAGCCATGAATTTTG+TGG | - | chr5.1:2714969-2714988 | None:intergenic | 25.0% |
| !! | TAACTTTTTAATAATCCACG+TGG | + | chr5.1:2713122-2713141 | MS.gene21928:intron | 25.0% |
| !!! | AAACAGTTTTTCAGATACTT+TGG | + | chr5.1:2713150-2713169 | MS.gene21928:intron | 25.0% |
| !!! | AAACTGAAACAGATTTTAGT+GGG | + | chr5.1:2713455-2713474 | MS.gene21928:intron | 25.0% |
| !!! | AACAGTTTTTCAGATACTTT+GGG | + | chr5.1:2713151-2713170 | MS.gene21928:intron | 25.0% |
| !!! | TAAGAGTTTATGGTGTTTTT+TGG | + | chr5.1:2714042-2714061 | MS.gene21928:intron | 25.0% |
| !!! | TTTTGAGAACTAAATCAAAC+AGG | - | chr5.1:2713231-2713250 | None:intergenic | 25.0% |
| AAACTATCCAATGATGAGTA+AGG | + | chr5.1:2713945-2713964 | MS.gene21928:intron | 30.0% | |
| AAAGAAGAAAAAGACCAAAG+AGG | + | chr5.1:2714098-2714117 | MS.gene21928:CDS | 30.0% | |
| AAAGAATATGGCATCTAACA+CGG | - | chr5.1:2714862-2714881 | None:intergenic | 30.0% | |
| AAATCACACCAAAGACATAT+TGG | - | chr5.1:2714811-2714830 | None:intergenic | 30.0% | |
| AACAAGCTCAAAAAAACAAC+TGG | + | chr5.1:2712996-2713015 | MS.gene21928:CDS | 30.0% | |
| AACTATCCAATGATGAGTAA+GGG | + | chr5.1:2713946-2713965 | MS.gene21928:intron | 30.0% | |
| ACAAGCTCAAAAAAACAACT+GGG | + | chr5.1:2712997-2713016 | MS.gene21928:CDS | 30.0% | |
| AGAAGAATACAAAACAGAGA+AGG | - | chr5.1:2713997-2714016 | None:intergenic | 30.0% | |
| ATAATCCAGTTTCTTCTGAT+AGG | + | chr5.1:2714393-2714412 | MS.gene21928:intron | 30.0% | |
| ATACCAAGTCGAAACTTAAA+GGG | - | chr5.1:2714509-2714528 | None:intergenic | 30.0% | |
| CCAACACAAATATAGACAAA+GGG | - | chr5.1:2713723-2713742 | None:intergenic | 30.0% | |
| GAAATTGTCCAATATGTCTT+TGG | + | chr5.1:2714800-2714819 | MS.gene21928:intron | 30.0% | |
| GAAGAATACAAAACAGAGAA+GGG | - | chr5.1:2713996-2714015 | None:intergenic | 30.0% | |
| GCTTATATTTGATGTTGAGT+TGG | + | chr5.1:2714980-2714999 | MS.gene21928:CDS | 30.0% | |
| GGTCAAACAAAATTACACAT+TGG | - | chr5.1:2714367-2714386 | None:intergenic | 30.0% | |
| GTTATAGAGGACATATCTAT+GGG | + | chr5.1:2714255-2714274 | MS.gene21928:CDS | 30.0% | |
| TATAACCAATCCATTTGGAT+AGG | - | chr5.1:2714241-2714260 | None:intergenic | 30.0% | |
| TCCAACACAAATATAGACAA+AGG | - | chr5.1:2713724-2713743 | None:intergenic | 30.0% | |
| TGCTAACTGTTAAGAGTTTA+TGG | + | chr5.1:2714032-2714051 | MS.gene21928:intron | 30.0% | |
| TTAAATGGGATGACTTTGAT+TGG | + | chr5.1:2714883-2714902 | MS.gene21928:intron | 30.0% | |
| ! | GAAATCCTTGTTTGATATTC+AGG | + | chr5.1:2714415-2714434 | MS.gene21928:intron | 30.0% |
| !! | ATATTTGATGTTGAGTTGGT+TGG | + | chr5.1:2714984-2715003 | MS.gene21928:CDS | 30.0% |
| !! | TTTATTTTCTTCAGGGATGA+GGG | + | chr5.1:2714733-2714752 | MS.gene21928:intron | 30.0% |
| !!! | CACTTCCAATTTTGTTTTCA+CGG | - | chr5.1:2713562-2713581 | None:intergenic | 30.0% |
| !!! | TTTTATTTTCTTCAGGGATG+AGG | + | chr5.1:2714732-2714751 | MS.gene21928:intron | 30.0% |
| AAGAATATGGCATCTAACAC+GGG | - | chr5.1:2714861-2714880 | None:intergenic | 35.0% | |
| AAGGCTGAAACAGAAACAAA+AGG | + | chr5.1:2712971-2712990 | MS.gene21928:CDS | 35.0% | |
| ACAATTTCATACCCCATAGA+TGG | - | chr5.1:2714788-2714807 | None:intergenic | 35.0% | |
| ACTGACCTGAATATCAAACA+AGG | - | chr5.1:2714423-2714442 | None:intergenic | 35.0% | |
| ACTTACAATTCCACCATCTA+TGG | + | chr5.1:2714772-2714791 | MS.gene21928:CDS | 35.0% | |
| CATACCAAGTCGAAACTTAA+AGG | - | chr5.1:2714510-2714529 | None:intergenic | 35.0% | |
| CCCTTTGTCTATATTTGTGT+TGG | + | chr5.1:2713720-2713739 | MS.gene21928:intron | 35.0% | |
| CTTACAATTCCACCATCTAT+GGG | + | chr5.1:2714773-2714792 | MS.gene21928:CDS | 35.0% | |
| GATTTCCTATCAGAAGAAAC+TGG | - | chr5.1:2714401-2714420 | None:intergenic | 35.0% | |
| GGAGTATACCACAAAATTCA+TGG | + | chr5.1:2714958-2714977 | MS.gene21928:CDS | 35.0% | |
| GGTTATAGAGGACATATCTA+TGG | + | chr5.1:2714254-2714273 | MS.gene21928:CDS | 35.0% | |
| TAAAGCTGTTGTAGAGATGT+TGG | - | chr5.1:2713587-2713606 | None:intergenic | 35.0% | |
| TATTTATCGTTTGCAGGTGT+AGG | + | chr5.1:2714594-2714613 | MS.gene21928:intron | 35.0% | |
| TCCTCTATAACCAATCCATT+TGG | - | chr5.1:2714246-2714265 | None:intergenic | 35.0% | |
| TGAAAACCCTTACTCATCAT+TGG | - | chr5.1:2713955-2713974 | None:intergenic | 35.0% | |
| TGTTCGTTGTTATGCAGATA+TGG | + | chr5.1:2714921-2714940 | MS.gene21928:intron | 35.0% | |
| TTACAATTCCACCATCTATG+GGG | + | chr5.1:2714774-2714793 | MS.gene21928:CDS | 35.0% | |
| TTCCCAAAACTGATGAATAG+AGG | - | chr5.1:2713333-2713352 | None:intergenic | 35.0% | |
| TTGCTCCGTGAAAACAAAAT+TGG | + | chr5.1:2713554-2713573 | MS.gene21928:intron | 35.0% | |
| ! | AACATCTTTTGCCTTCTTCT+TGG | - | chr5.1:2713027-2713046 | None:intergenic | 35.0% |
| ! | ACTCCTCTATTCATCAGTTT+TGG | + | chr5.1:2713327-2713346 | MS.gene21928:intron | 35.0% |
| ! | CAAGTTGAAAGATCATGGTA+AGG | + | chr5.1:2713806-2713825 | MS.gene21928:intron | 35.0% |
| ! | CTCCTCTATTCATCAGTTTT+GGG | + | chr5.1:2713328-2713347 | MS.gene21928:intron | 35.0% |
| !! | AAAACTGTTTTGAATCCACG+TGG | - | chr5.1:2713140-2713159 | None:intergenic | 35.0% |
| !! | AGATTTAGCTTTGTCTGAAG+TGG | - | chr5.1:2712942-2712961 | None:intergenic | 35.0% |
| !! | TCCAAATGGATTGGTTATAG+AGG | + | chr5.1:2714242-2714261 | MS.gene21928:CDS | 35.0% |
| !!! | GAAACAGATTTTAGTGGGTT+TGG | + | chr5.1:2713460-2713479 | MS.gene21928:intron | 35.0% |
| !!! | GGGTTTGGTTTTCAGTTTTT+TGG | + | chr5.1:2713475-2713494 | MS.gene21928:intron | 35.0% |
| !!! | TCTTGTTTTTCTTCTCAACG+AGG | - | chr5.1:2714074-2714093 | None:intergenic | 35.0% |
| AAAAAGACCAAAGAGGCTGA+AGG | + | chr5.1:2714105-2714124 | MS.gene21928:CDS | 40.0% | |
| AAAAGTTCAAGCTGTCGATG+AGG | + | chr5.1:2713070-2713089 | MS.gene21928:CDS | 40.0% | |
| AACGTGAGAACCTATCCAAA+TGG | + | chr5.1:2714228-2714247 | MS.gene21928:CDS | 40.0% | |
| AACTGGGAAATCCAAGAAGA+AGG | + | chr5.1:2713013-2713032 | MS.gene21928:CDS | 40.0% | |
| AAGAGCTGATCTTGGAAAGA+AGG | + | chr5.1:2714293-2714312 | MS.gene21928:CDS | 40.0% | |
| AATGTCTCTCATGCAAAGGA+TGG | + | chr5.1:2713912-2713931 | MS.gene21928:intron | 40.0% | |
| AGATATGGTGACAAACGTGT+TGG | + | chr5.1:2714936-2714955 | MS.gene21928:intron | 40.0% | |
| AGCTAAATCTGAGTCTCAGA+AGG | + | chr5.1:2712952-2712971 | MS.gene21928:CDS | 40.0% | |
| ATATCTATGGGCAAACCAGA+TGG | + | chr5.1:2714267-2714286 | MS.gene21928:CDS | 40.0% | |
| ATGAGAGACATTGTTCTGGT+TGG | - | chr5.1:2713904-2713923 | None:intergenic | 40.0% | |
| ATGCAAAGGATGGAGATGAT+GGG | + | chr5.1:2713922-2713941 | MS.gene21928:intron | 40.0% | |
| ATTTCATACCCCATAGATGG+TGG | - | chr5.1:2714785-2714804 | None:intergenic | 40.0% | |
| CAGGTCAGTGTTAGATATAC+CGG | + | chr5.1:2714434-2714453 | MS.gene21928:intron | 40.0% | |
| GAACAATGTCTCTCATGCAA+AGG | + | chr5.1:2713908-2713927 | MS.gene21928:intron | 40.0% | |
| GAGAACCTATCCAAATGGAT+TGG | + | chr5.1:2714233-2714252 | MS.gene21928:CDS | 40.0% | |
| GATATGGTGACAAACGTGTT+GGG | + | chr5.1:2714937-2714956 | MS.gene21928:intron | 40.0% | |
| GTTGAAAGATCATGGTAAGG+AGG | + | chr5.1:2713809-2713828 | MS.gene21928:intron | 40.0% | |
| TTGCATGAGAGACATTGTTC+TGG | - | chr5.1:2713908-2713927 | None:intergenic | 40.0% | |
| ! | ACTTTGGGCCTGTTTGATTT+AGG | + | chr5.1:2713166-2713185 | MS.gene21928:intron | 40.0% |
| ! | CTTTGTCTGAAGTGGAAATC+GGG | - | chr5.1:2712934-2712953 | None:intergenic | 40.0% |
| ! | GCTTTGTCTGAAGTGGAAAT+CGG | - | chr5.1:2712935-2712954 | None:intergenic | 40.0% |
| ACCGGCAAGCTACAAAAAGA+TGG | + | chr5.1:2714452-2714471 | MS.gene21928:CDS | 45.0% | |
| AGGTGTAGGACAAGTCATCA+AGG | + | chr5.1:2714608-2714627 | MS.gene21928:intron | 45.0% | |
| CATGCAAAGGATGGAGATGA+TGG | + | chr5.1:2713921-2713940 | MS.gene21928:intron | 45.0% | |
| CCGGCAAGCTACAAAAAGAT+GGG | + | chr5.1:2714453-2714472 | MS.gene21928:CDS | 45.0% | |
| GATGGCAAAAGAGCTGATCT+TGG | + | chr5.1:2714285-2714304 | MS.gene21928:CDS | 45.0% | |
| GGATAGGTTCTCACGTTAGA+TGG | - | chr5.1:2714225-2714244 | None:intergenic | 45.0% | |
| GGTGTAGGACAAGTCATCAA+GGG | + | chr5.1:2714609-2714628 | MS.gene21928:intron | 45.0% | |
| GTGTAGGACAAGTCATCAAG+GGG | + | chr5.1:2714610-2714629 | MS.gene21928:CDS | 45.0% | |
| TGTGCCAGATGAAAAGTCTG+AGG | + | chr5.1:2713863-2713882 | MS.gene21928:intron | 45.0% | |
| TTTCTTCAGGGATGAGGGTT+GGG | + | chr5.1:2714738-2714757 | MS.gene21928:intron | 45.0% | |
| ! | ACCTCCTCAGACTTTTCATC+TGG | - | chr5.1:2713870-2713889 | None:intergenic | 45.0% |
| ! | AGATCAGCTCTTTTGCCATC+TGG | - | chr5.1:2714285-2714304 | None:intergenic | 45.0% |
| ! | GCACCCTTTAAGTTTCGACT+TGG | + | chr5.1:2714503-2714522 | MS.gene21928:CDS | 45.0% |
| ! | TCAAACAGGCCCAAAGTATC+TGG | - | chr5.1:2713163-2713182 | None:intergenic | 45.0% |
| ! | TTAGATGGCTTGGCTTCAGT+TGG | - | chr5.1:2714210-2714229 | None:intergenic | 45.0% |
| ! | TTTTCTTCAGGGATGAGGGT+TGG | + | chr5.1:2714737-2714756 | MS.gene21928:intron | 45.0% |
| !! | CCCATCTTTTTGTAGCTTGC+CGG | - | chr5.1:2714456-2714475 | None:intergenic | 45.0% |
| !! | GGAGGAAAGTGCTCATGTAA+AGG | + | chr5.1:2713827-2713846 | MS.gene21928:intron | 45.0% |
| AGCTTCTCCTTCAGCCTCTT+TGG | - | chr5.1:2714115-2714134 | None:intergenic | 50.0% | |
| AGGACAAGTCATCAAGGGGT+GGG | + | chr5.1:2714614-2714633 | MS.gene21928:CDS | 50.0% | |
| AGGATGAGCAGCTGCAAGAT+GGG | + | chr5.1:2713090-2713109 | MS.gene21928:CDS | 50.0% | |
| GCCAGATGAAAAGTCTGAGG+AGG | + | chr5.1:2713866-2713885 | MS.gene21928:intron | 50.0% | |
| GGTTCTCACGTTAGATGGCT+TGG | - | chr5.1:2714220-2714239 | None:intergenic | 50.0% | |
| TAGGACAAGTCATCAAGGGG+TGG | + | chr5.1:2714613-2714632 | MS.gene21928:CDS | 50.0% | |
| TTCTTCAGGGATGAGGGTTG+GGG | + | chr5.1:2714739-2714758 | MS.gene21928:intron | 50.0% | |
| !! | GTTGAGTTGGTTGGCGTTGA+AGG | + | chr5.1:2714993-2715012 | MS.gene21928:CDS | 50.0% |
| GAGGATGAGCAGCTGCAAGA+TGG | + | chr5.1:2713089-2713108 | MS.gene21928:CDS | 55.0% | |
| GTCATCAAGGGGTGGGATGT+TGG | + | chr5.1:2714621-2714640 | MS.gene21928:CDS | 55.0% | |
| GGGTGGGATGTTGGCGTCAA+TGG | + | chr5.1:2714630-2714649 | MS.gene21928:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 2712924 | 2715022 | 2712924 | ID=MS.gene21928 |
| chr5.1 | mRNA | 2712924 | 2715022 | 2712924 | ID=MS.gene21928.t1;Parent=MS.gene21928 |
| chr5.1 | exon | 2712924 | 2713111 | 2712924 | ID=MS.gene21928.t1.exon1;Parent=MS.gene21928.t1 |
| chr5.1 | CDS | 2712924 | 2713111 | 2712924 | ID=cds.MS.gene21928.t1;Parent=MS.gene21928.t1 |
| chr5.1 | exon | 2714068 | 2714314 | 2714068 | ID=MS.gene21928.t1.exon2;Parent=MS.gene21928.t1 |
| chr5.1 | CDS | 2714068 | 2714314 | 2714068 | ID=cds.MS.gene21928.t1;Parent=MS.gene21928.t1 |
| chr5.1 | exon | 2714437 | 2714524 | 2714437 | ID=MS.gene21928.t1.exon3;Parent=MS.gene21928.t1 |
| chr5.1 | CDS | 2714437 | 2714524 | 2714437 | ID=cds.MS.gene21928.t1;Parent=MS.gene21928.t1 |
| chr5.1 | exon | 2714610 | 2714651 | 2714610 | ID=MS.gene21928.t1.exon4;Parent=MS.gene21928.t1 |
| chr5.1 | CDS | 2714610 | 2714651 | 2714610 | ID=cds.MS.gene21928.t1;Parent=MS.gene21928.t1 |
| chr5.1 | exon | 2714747 | 2714795 | 2714747 | ID=MS.gene21928.t1.exon5;Parent=MS.gene21928.t1 |
| chr5.1 | CDS | 2714747 | 2714795 | 2714747 | ID=cds.MS.gene21928.t1;Parent=MS.gene21928.t1 |
| chr5.1 | exon | 2714938 | 2715022 | 2714938 | ID=MS.gene21928.t1.exon6;Parent=MS.gene21928.t1 |
| chr5.1 | CDS | 2714938 | 2715022 | 2714938 | ID=cds.MS.gene21928.t1;Parent=MS.gene21928.t1 |
| Gene Sequence |
| Protein sequence |
