AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24633


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAGAAAAAGAAACATT+TGG - 6095:47169-47188 MS.gene24633:intron 15.0%
!!! AAAAAAAAGAAGTTGATTTA+AGG + 6095:47610-47629 None:intergenic 15.0%
!!! AATAAATCTATTGATTTGTT+GGG + 6095:47202-47221 None:intergenic 15.0%
!!! GAAAATGAAAATTTTAAAAC+TGG + 6095:46818-46837 None:intergenic 15.0%
!!! TAATAAATCTATTGATTTGT+TGG + 6095:47203-47222 None:intergenic 15.0%
!! AAAAAGAAACATTTGGTTAT+GGG - 6095:47176-47195 MS.gene24633:intron 20.0%
!! AAAGGACATCTTAATATAAA+TGG + 6095:47115-47134 None:intergenic 20.0%
!! AACACTTGAATATAAATAAC+AGG + 6095:47141-47160 None:intergenic 20.0%
!! AATATAAATAACAGGAGAAA+AGG + 6095:47133-47152 None:intergenic 20.0%
!! ACTATATATGCATATATTGT+TGG - 6095:47943-47962 MS.gene24633:intron 20.0%
!!! AATGAAAATTTTAAAACTGG+AGG + 6095:46815-46834 None:intergenic 20.0%
!!! CATCATTTTTATTCTTTTGA+AGG - 6095:47323-47342 MS.gene24633:intron 20.0%
! AAAAAAATCAGGTATAGCAA+GGG + 6095:47686-47705 None:intergenic 25.0%
! AATTTAGAAATCAAACTGGT+TGG + 6095:47574-47593 None:intergenic 25.0%
! AGATTTAGAGATTAATGAGT+TGG - 6095:46555-46574 MS.gene24633:intron 25.0%
! ATGATTCTAAACCAGTAAAT+TGG - 6095:47423-47442 MS.gene24633:intron 25.0%
! CTACAAAAGAAATATTCGAT+TGG - 6095:47734-47753 MS.gene24633:intron 25.0%
! GAAAAAGAAACATTTGGTTA+TGG - 6095:47175-47194 MS.gene24633:intron 25.0%
! GATGTAAATGTTAACTTCAA+AGG - 6095:46986-47005 MS.gene24633:intron 25.0%
! TCTAGATTCAACACAAAAAA+AGG + 6095:47898-47917 None:intergenic 25.0%
! TGGTAATTTAGAAATCAAAC+TGG + 6095:47578-47597 None:intergenic 25.0%
! TGGTCTATGATAGAATATTA+TGG - 6095:46590-46609 MS.gene24633:intron 25.0%
! TTGATTTAAGGAAAGAAACT+TGG + 6095:47598-47617 None:intergenic 25.0%
!! TTCAGCATCTTTTATTATCT+TGG - 6095:47520-47539 MS.gene24633:intron 25.0%
AAAAAGGTGATCACTTACAA+TGG + 6095:47882-47901 None:intergenic 30.0%
AAACAGACTTCATTGTTAAC+AGG - 6095:47388-47407 MS.gene24633:intron 30.0%
AAACTTCCTGTGTTATATAC+CGG - 6095:47635-47654 MS.gene24633:CDS 30.0%
AAATTAGGCTATTGAAGGAA+GGG - 6095:48286-48305 MS.gene24633:CDS 30.0%
AACAGACTTCATTGTTAACA+GGG - 6095:47389-47408 MS.gene24633:intron 30.0%
ACAACAATGGGAAAAAAATC+AGG + 6095:47697-47716 None:intergenic 30.0%
ACATTATGTGTTCTGACTTT+GGG - 6095:48108-48127 MS.gene24633:intron 30.0%
AGAAGAAATTAGGCTATTGA+AGG - 6095:48281-48300 MS.gene24633:CDS 30.0%
ATCAAAACTAAGCATGATTC+CGG - 6095:46859-46878 MS.gene24633:intron 30.0%
ATCTTTCAGCAAATTGTAAG+TGG - 6095:47042-47061 MS.gene24633:intron 30.0%
CAATTACTTGACCAATTTAC+TGG + 6095:47437-47456 None:intergenic 30.0%
CAGTAAATTGGTCAAGTAAT+TGG - 6095:47435-47454 MS.gene24633:intron 30.0%
CCAAGTTCAATATCTTTCAA+CGG + 6095:48324-48343 None:intergenic 30.0%
CTGAGATAGTAATCAACAAA+TGG + 6095:47821-47840 None:intergenic 30.0%
GAAAAAAATCAGGTATAGCA+AGG + 6095:47687-47706 None:intergenic 30.0%
GAACACATAATGTATTCATG+TGG + 6095:48101-48120 None:intergenic 30.0%
TACATTACAAAACACAGCTA+AGG + 6095:48025-48044 None:intergenic 30.0%
TACATTATGTGTTCTGACTT+TGG - 6095:48107-48126 MS.gene24633:intron 30.0%
TATCATAGACCACCATATTT+CGG + 6095:46582-46601 None:intergenic 30.0%
TCATTACTACAGAATCTACA+GGG + 6095:46456-46475 None:intergenic 30.0%
TTTCAAGAAACATCATGGAA+TGG - 6095:48158-48177 MS.gene24633:intron 30.0%
! AGTGTCTTTTAAATTTCTGC+AGG - 6095:47978-47997 MS.gene24633:intron 30.0%
! GATCTAAAATGAACATTGAC+TGG + 6095:47368-47387 None:intergenic 30.0%
! TTTGTTGATTACTATCTCAG+TGG - 6095:47821-47840 MS.gene24633:intron 30.0%
AATTAGGCTATTGAAGGAAG+GGG - 6095:48287-48306 MS.gene24633:CDS 35.0%
ACAGACTTCATTGTTAACAG+GGG - 6095:47390-47409 MS.gene24633:intron 35.0%
ACCAGAAGACATCTTAAAGT+TGG + 6095:47858-47877 None:intergenic 35.0%
AGATGAAAAGCAAGCATACT+TGG + 6095:46387-46406 None:intergenic 35.0%
ATAAGAGAGAGAGCTTGAAT+AGG + 6095:47788-47807 None:intergenic 35.0%
ATGCTTAGAAGCAGAATAAC+CGG + 6095:46269-46288 None:intergenic 35.0%
CATTACTACAGAATCTACAG+GGG + 6095:46455-46474 None:intergenic 35.0%
CATTATGTGTTCTGACTTTG+GGG - 6095:48109-48128 MS.gene24633:intron 35.0%
CCAACAAATAGCATGATTTC+CGG + 6095:47657-47676 None:intergenic 35.0%
CCATTGTTGTGATAGTCAAT+CGG - 6095:47707-47726 MS.gene24633:intron 35.0%
CCGTTGAAAGATATTGAACT+TGG - 6095:48321-48340 MS.gene24633:CDS 35.0%
CGATTGACTATCACAACAAT+GGG + 6095:47709-47728 None:intergenic 35.0%
CGGCATGGTAATTTATAGTT+GGG - 6095:48194-48213 MS.gene24633:CDS 35.0%
CTCACTTTCAAGAAACATCA+TGG - 6095:48153-48172 MS.gene24633:intron 35.0%
GAAAGATATTGAACTTGGTC+AGG - 6095:48326-48345 MS.gene24633:CDS 35.0%
GAAATTAGGCTATTGAAGGA+AGG - 6095:48285-48304 MS.gene24633:CDS 35.0%
GCCTAACAGAAGAAGAAATT+AGG - 6095:48271-48290 MS.gene24633:CDS 35.0%
GCCTAATTTCTTCTTCTGTT+AGG + 6095:48275-48294 None:intergenic 35.0%
GCTCTCTCTCTTATTATTCT+TGG - 6095:47794-47813 MS.gene24633:intron 35.0%
GTCATTACTACAGAATCTAC+AGG + 6095:46457-46476 None:intergenic 35.0%
GTTGCCAATATATCTATCAC+CGG - 6095:46322-46341 MS.gene24633:CDS 35.0%
GTTTCTCTTATAGATTTCCC+AGG + 6095:46526-46545 None:intergenic 35.0%
TAAATTCCATATTGCCCACA+TGG + 6095:47089-47108 None:intergenic 35.0%
TACAGACACATCTTTCATCT+GGG - 6095:47221-47240 MS.gene24633:intron 35.0%
TCGGCATGGTAATTTATAGT+TGG - 6095:48193-48212 MS.gene24633:CDS 35.0%
TGATTTCCGGTATATAACAC+AGG + 6095:47644-47663 None:intergenic 35.0%
TTACAGACACATCTTTCATC+TGG - 6095:47220-47239 MS.gene24633:intron 35.0%
TTCTAAGCATGTTCCTTTGT+GGG - 6095:46279-46298 MS.gene24633:CDS 35.0%
TTGAATTGAAGATGAGTGCT+AGG - 6095:47267-47286 MS.gene24633:intron 35.0%
TTGCCAATATATCTATCACC+GGG - 6095:46323-46342 MS.gene24633:CDS 35.0%
! TAATGAGTTGGACCGAAATA+TGG - 6095:46567-46586 MS.gene24633:intron 35.0%
!! ACCAACTTTAAGATGTCTTC+TGG - 6095:47854-47873 MS.gene24633:intron 35.0%
!!! TTAGGCTGTTTTTCGAATGA+GGG + 6095:48257-48276 None:intergenic 35.0%
AAAACTAAGCATGATTCCGG+TGG - 6095:46862-46881 MS.gene24633:intron 40.0%
AACCAAATAAGCTCCCACAA+AGG + 6095:46295-46314 None:intergenic 40.0%
ATGGTTGTGGTTATTGGTGT+TGG - 6095:46947-46966 MS.gene24633:intron 40.0%
CATCCCGGTGATAGATATAT+TGG + 6095:46329-46348 None:intergenic 40.0%
CCGATTGACTATCACAACAA+TGG + 6095:47710-47729 None:intergenic 40.0%
CTTCTAAGCATGTTCCTTTG+TGG - 6095:46278-46297 MS.gene24633:CDS 40.0%
GAAAGTGAAAACACGAGAGT+AGG + 6095:46781-46800 None:intergenic 40.0%
GCATACTTGGTAGAATCCAA+CGG + 6095:46374-46393 None:intergenic 40.0%
GGTAATTTATAGTTGGGCTG+TGG - 6095:48200-48219 MS.gene24633:CDS 40.0%
TATTCATGTGGCCTAAAACC+TGG + 6095:48089-48108 None:intergenic 40.0%
TCAATCCTTAACCCACTAAC+CGG + 6095:46641-46660 None:intergenic 40.0%
TCACAGCAAACACTACTCAA+GGG - 6095:46907-46926 MS.gene24633:intron 40.0%
TGATGATACAACTGCTACAC+AGG + 6095:47023-47042 None:intergenic 40.0%
TTCACAGCAAACACTACTCA+AGG - 6095:46906-46925 MS.gene24633:intron 40.0%
TTCCTTTGTGGGAGCTTATT+TGG - 6095:46290-46309 MS.gene24633:CDS 40.0%
! CCGGAAATCATGCTATTTGT+TGG - 6095:47654-47673 MS.gene24633:CDS 40.0%
! TTTGCTGTAACTGCTCTTGT+CGG - 6095:46226-46245 MS.gene24633:CDS 40.0%
!! CTTCCTTTTAGAGACATGTG+CGG - 6095:46708-46727 MS.gene24633:CDS 40.0%
!! TTGAGTAGTGTTTGCTGTGA+AGG + 6095:46907-46926 None:intergenic 40.0%
!!! GTTAGGCTGTTTTTCGAATG+AGG + 6095:48258-48277 None:intergenic 40.0%
AATGTGTCTGTTCCACCATG+TGG - 6095:47071-47090 MS.gene24633:intron 45.0%
ATCCAACGGAATTGAGCATG+AGG + 6095:46360-46379 None:intergenic 45.0%
ATGTGTCTGTTCCACCATGT+GGG - 6095:47072-47091 MS.gene24633:intron 45.0%
ATTCCATATTGCCCACATGG+TGG + 6095:47086-47105 None:intergenic 45.0%
CATGAGGCTTAAGTTCATCC+CGG + 6095:46344-46363 None:intergenic 45.0%
CATTCCATCACACAAACCAC+CGG + 6095:46881-46900 None:intergenic 45.0%
CTACCGCACATGTCTCTAAA+AGG + 6095:46714-46733 None:intergenic 45.0%
GGAATGGTTCTTGCAGTTGT+CGG - 6095:48174-48193 MS.gene24633:intron 45.0%
TAAGCCAGTACCTCTGCATT+GGG - 6095:48043-48062 MS.gene24633:intron 45.0%
TCGGTCATGGTTGTGGTTAT+TGG - 6095:46941-46960 MS.gene24633:intron 45.0%
TGTTCATCAGCACTATTCGC+TGG + 6095:47468-47487 None:intergenic 45.0%
! AGATTTCCCAGGTTTTCCAC+TGG + 6095:46515-46534 None:intergenic 45.0%
! CTCAAGGGAAGTGAAAGTGT+CGG - 6095:46922-46941 MS.gene24633:intron 45.0%
! GCAGTTTCCTTCCAGGTTTT+AGG - 6095:48075-48094 MS.gene24633:intron 45.0%
! TATGTGTTCTGACTTTGGGG+TGG - 6095:48112-48131 MS.gene24633:intron 45.0%
! TGAGTTGGACCGAAATATGG+TGG - 6095:46570-46589 MS.gene24633:intron 45.0%
ACTACAGCCAGTGGAAAACC+TGG - 6095:46505-46524 MS.gene24633:CDS 50.0%
AGCCTCATGCTCAATTCCGT+TGG - 6095:46355-46374 MS.gene24633:CDS 50.0%
AGTCAGATCACTACAGCCAG+TGG - 6095:46496-46515 MS.gene24633:CDS 50.0%
CATGTGGCCTAAAACCTGGA+AGG + 6095:48085-48104 None:intergenic 50.0%
CTACAGCCAGTGGAAAACCT+GGG - 6095:46506-46525 MS.gene24633:CDS 50.0%
CTTAACCCACTAACCGGAGT+CGG + 6095:46635-46654 None:intergenic 50.0%
CTTCTCAGCAGTTTCCTTCC+AGG - 6095:48068-48087 MS.gene24633:intron 50.0%
GGACTCGTCTCAAATGCTAC+CGG - 6095:46247-46266 MS.gene24633:CDS 50.0%
GTAAGCCAGTACCTCTGCAT+TGG - 6095:48042-48061 MS.gene24633:intron 50.0%
GTTCCACCATGTGGGCAATA+TGG - 6095:47080-47099 MS.gene24633:intron 50.0%
TGAGGGAGAGTCTTCGCATT+TGG + 6095:48240-48259 None:intergenic 50.0%
! CGGTGGTTTGTGTGATGGAA+TGG - 6095:46879-46898 MS.gene24633:intron 50.0%
! GATTCCGGTGGTTTGTGTGA+TGG - 6095:46874-46893 MS.gene24633:intron 50.0%
! TTGATCCATGTAGCCGACTC+CGG - 6095:46619-46638 MS.gene24633:intron 50.0%
!! GAAAGTGTCGGTCATGGTTG+TGG - 6095:46934-46953 MS.gene24633:intron 50.0%
!! GGAAGTGAAAGTGTCGGTCA+TGG - 6095:46928-46947 MS.gene24633:intron 50.0%
ACTAACCGGAGTCGGCTACA+TGG + 6095:46627-46646 None:intergenic 55.0%
GGTTCTTGCAGTTGTCGGCA+TGG - 6095:48179-48198 MS.gene24633:CDS 55.0%
! ATGTAGCCGACTCCGGTTAG+TGG - 6095:46626-46645 MS.gene24633:intron 55.0%
! TGTAGCCGACTCCGGTTAGT+GGG - 6095:46627-46646 MS.gene24633:intron 55.0%
!! CGACTCCGGTTAGTGGGTTA+AGG - 6095:46633-46652 MS.gene24633:intron 55.0%
! AGCGCCCAATGCAGAGGTAC+TGG + 6095:48050-48069 None:intergenic 60.0%
!! CTGAGAAGCGCCCAATGCAG+AGG + 6095:48056-48075 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
6095 gene 46203 48359 46203 ID=MS.gene24633
6095 mRNA 46203 48359 46203 ID=MS.gene24633.t1;Parent=MS.gene24633
6095 exon 48175 48359 48175 ID=MS.gene24633.t1.exon1;Parent=MS.gene24633.t1
6095 CDS 48175 48359 48175 ID=cds.MS.gene24633.t1;Parent=MS.gene24633.t1
6095 exon 47529 47705 47529 ID=MS.gene24633.t1.exon2;Parent=MS.gene24633.t1
6095 CDS 47529 47705 47529 ID=cds.MS.gene24633.t1;Parent=MS.gene24633.t1
6095 exon 46679 46840 46679 ID=MS.gene24633.t1.exon3;Parent=MS.gene24633.t1
6095 CDS 46679 46840 46679 ID=cds.MS.gene24633.t1;Parent=MS.gene24633.t1
6095 exon 46203 46562 46203 ID=MS.gene24633.t1.exon4;Parent=MS.gene24633.t1
6095 CDS 46203 46562 46203 ID=cds.MS.gene24633.t1;Parent=MS.gene24633.t1
Gene Sequence

>MS.gene24633

CGACTACTTTGACTGGGTTTCACTTTGCTGTAACTGCTCTTGTCGGACTCGTCTCAAATGCTACCGGTTATTCTGCTTCTAAGCATGTTCCTTTGTGGGAGCTTATTTGGTTCTCTGCTGTTGCCAATATATCTATCACCGGGATGAACTTAAGCCTCATGCTCAATTCCGTTGGATTCTACCAAGTATGCTTGCTTTTCATCTTTACTCTTTCAACTTCATTCAATTTTCAAGATGTTAGAGAGAAAACCCCTGTAGATTCTGTAGTAATGACAGTAGATCAGATGAAACATAGTCAGATCACTACAGCCAGTGGAAAACCTGGGAAATCTATAAGAGAAACTATTAAAAAAGATTTAGAGATTAATGAGTTGGACCGAAATATGGTGGTCTATGATAGAATATTATGGCATAATTTGATCCATGTAGCCGACTCCGGTTAGTGGGTTAAGGATTGATTGATTGATTGTCTGCATCTTGTAATAATTTCAAATTATGCTCAACTCTTCCTTTTAGAGACATGTGCGGTAGTTATTATAATTACATCTCATGTGCTCTAACTTTTAAATGAGCAGCCTACTCTCGTGTTTTCACTTTCAATCTTCATTTCCTCCAGTTTTAAAATTTTCATTTTCTCATCGTGTATGTGAACAGATATCAAAACTAAGCATGATTCCGGTGGTTTGTGTGATGGAATGGATCCTTCACAGCAAACACTACTCAAGGGAAGTGAAAGTGTCGGTCATGGTTGTGGTTATTGGTGTTGGTGTTTGCACTGTAACTGATGTAAATGTTAACTTCAAAGGTTTTGTGTGCGCCTGTGTAGCAGTTGTATCATCATCTTTCAGCAAATTGTAAGTGGTATATAAATGTGTCTGTTCCACCATGTGGGCAATATGGAATTTATCTCCATTTATATTAAGATGTCCTTTTCTCCTGTTATTTATATTCAAGTGTTGTACTACGATAATAGAAAAAGAAACATTTGGTTATGGGCCCAACAAATCAATAGATTTATTACAGACACATCTTTCATCTGGGATTTTTTAATTCTTTTGTACATTTTGAATTGAAGATGAGTGCTAGGTGAAACTTGCATAGACGTAGTATCATGAGTGAGCATCATTTTTATTCTTTTGAAGGTTTTTTAACTTGTACTTTGCCAGTCAATGTTCATTTTAGATCAAACAGACTTCATTGTTAACAGGGGAGTAGAAAACATGATTCTAAACCAGTAAATTGGTCAAGTAATTGGTTCAAAACCAGCGAATAGTGCTGATGAACACTGTTGTTTTTCATTTTTTTTATCTTTTTTTTTTCAGCATCTTTTATTATCTTGGTAATCATTTTATTTGAATTTCATAATAACCAACCAGTTTGATTTCTAAATTACCAAGTTTCTTTCCTTAAATCAACTTCTTTTTTTTACAAGAAACTTCCTGTGTTATATACCGGAAATCATGCTATTTGTTGGATTGATCCCTTGCTATACCTGATTTTTTTCCCATTGTTGTGATAGTCAATCGGTTCTCTACAAAAGAAATATTCGATTGGATCTTTTGAACTGCTGAGTAAGACTGCTCCTATTCAAGCTCTCTCTCTTATTATTCTTGGTCCATTTGTTGATTACTATCTCAGTGGCAAGTTGATAACCAACTTTAAGATGTCTTCTGGTGCCATTGTAAGTGATCACCTTTTTTTGTGTTGAATCTAGATAGTTTCGCTTTGTATTTGTTGTTAACTATATATGCATATATTGTTGGATTGTTTATTAAAGTGTCTTTTAAATTTCTGCAGGTTTTCATTCTTCTTTCATGCACCTTAGCTGTGTTTTGTAATGTAAGCCAGTACCTCTGCATTGGGCGCTTCTCAGCAGTTTCCTTCCAGGTTTTAGGCCACATGAATACATTATGTGTTCTGACTTTGGGGTGGCTGTTATTCGATTCAGAGCTCACTTTCAAGAAACATCATGGAATGGTTCTTGCAGTTGTCGGCATGGTAATTTATAGTTGGGCTGTGGAGCTTGAAAAGCAGCCAAATGCGAAGACTCTCCCTCATTCGAAAAACAGCCTAACAGAAGAAGAAATTAGGCTATTGAAGGAAGGGGTAGAAAATAGTCCGTTGAAAGATATTGAACTTGGTCAGGCTAAAGTTTAG

Protein sequence

>MS.gene24633.t1

RLLULGFTLLULLLSDSSQMLPVILLLSMFLCGSLFGSLLLPIYLSPGUTUASCSIPLDSTKYQNUAUFRWFVUWNGSFTANTTQGKUKCRSWLWLLVLVFALULMUMLTSKVLCAPVUQFQSVLYKRNIRLDLLNCUVRLLLFKLSLLLFLVHLLITISVASUUPTLRCLLVPLFSFFFHAPULCFVMUASTSALGASQQFPSRFUATUIHYVFULWGGCYSIQSSLSRNIMEWFLQLSAWUFIVGLWSLKSSQMRRLSLIRKTAUQKKKLGYURKGUKIVRUKILNLVRLKF