AlfalfaGEDB | MS.gene27271

M. sativa cultivar XinJiangDaYe / MS.gene27271

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27271.t1	AUS45886.1	99.1	117	1	0	2	118	3	119	5.40E-58	233.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27271.t1	Q43636	71.8	117	32	1	2	118	3	118	1.4e-43	176.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27271.t1	A0A2I7VTB4	99.1	117	1	0	2	118	3	119	3.9e-58	233.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene27271	0.829111	6.18E-55	-1.69E-46
MS.gene050226	MS.gene27271	0.809116	2.22E-50	-1.69E-46
MS.gene050227	MS.gene27271	0.800766	1.24E-48	-1.69E-46
MS.gene050442	MS.gene27271	0.845983	2.89E-59	-1.69E-46
MS.gene050605	MS.gene27271	0.803647	3.15E-49	-1.69E-46
MS.gene050680	MS.gene27271	0.845761	3.32E-59	-1.69E-46
MS.gene050750	MS.gene27271	0.826539	2.57E-54	-1.69E-46
MS.gene050762	MS.gene27271	0.811314	7.45E-51	-1.69E-46
MS.gene050763	MS.gene27271	0.812086	5.06E-51	-1.69E-46
MS.gene050826	MS.gene27271	0.838028	3.67E-57	-1.69E-46
MS.gene050953	MS.gene27271	0.821274	4.41E-53	-1.69E-46
MS.gene051265	MS.gene27271	0.800355	1.50E-48	-1.69E-46
MS.gene052176	MS.gene27271	0.812062	5.12E-51	-1.69E-46
MS.gene052333	MS.gene27271	0.817286	3.57E-52	-1.69E-46
MS.gene052426	MS.gene27271	0.84226	2.88E-58	-1.69E-46
MS.gene052674	MS.gene27271	0.804019	2.64E-49	-1.69E-46
MS.gene052675	MS.gene27271	0.803707	3.07E-49	-1.69E-46
MS.gene052677	MS.gene27271	0.816435	5.54E-52	-1.69E-46
MS.gene05288	MS.gene27271	0.803229	3.85E-49	-1.69E-46
MS.gene053166	MS.gene27271	0.86062	1.82E-63	-1.69E-46
MS.gene053494	MS.gene27271	0.875752	2.40E-68	-1.69E-46
MS.gene053584	MS.gene27271	0.811765	5.94E-51	-1.69E-46
MS.gene054236	MS.gene27271	0.814093	1.84E-51	-1.69E-46
MS.gene054379	MS.gene27271	0.828566	8.37E-55	-1.69E-46
MS.gene054405	MS.gene27271	0.813877	2.05E-51	-1.69E-46
MS.gene054468	MS.gene27271	0.807517	4.86E-50	-1.69E-46
MS.gene054542	MS.gene27271	0.806229	9.10E-50	-1.69E-46
MS.gene054559	MS.gene27271	0.840789	7.04E-58	-1.69E-46
MS.gene054930	MS.gene27271	0.854967	8.65E-62	-1.69E-46
MS.gene05510	MS.gene27271	0.873382	1.54E-67	-1.69E-46
MS.gene055227	MS.gene27271	0.800685	1.28E-48	-1.69E-46
MS.gene055356	MS.gene27271	0.838778	2.35E-57	-1.69E-46
MS.gene055358	MS.gene27271	0.829459	5.09E-55	-1.69E-46
MS.gene055367	MS.gene27271	0.802566	5.28E-49	-1.69E-46
MS.gene055623	MS.gene27271	0.815611	8.46E-52	-1.69E-46
MS.gene055701	MS.gene27271	0.881799	1.78E-70	-1.69E-46
MS.gene055818	MS.gene27271	0.809111	2.22E-50	-1.69E-46
MS.gene055926	MS.gene27271	0.82749	1.52E-54	-1.69E-46
MS.gene05595	MS.gene27271	0.812761	3.60E-51	-1.69E-46
MS.gene056480	MS.gene27271	0.808685	2.74E-50	-1.69E-46
MS.gene056873	MS.gene27271	0.814462	1.52E-51	-1.69E-46
MS.gene057156	MS.gene27271	-0.822427	2.38E-53	-1.69E-46
MS.gene057194	MS.gene27271	0.840231	9.85E-58	-1.69E-46
MS.gene057394	MS.gene27271	0.833481	5.20E-56	-1.69E-46
MS.gene057469	MS.gene27271	0.813057	3.10E-51	-1.69E-46
MS.gene05761	MS.gene27271	0.85225	5.22E-61	-1.69E-46
MS.gene057639	MS.gene27271	0.803336	3.66E-49	-1.69E-46
MS.gene057750	MS.gene27271	0.830006	3.74E-55	-1.69E-46
MS.gene057929	MS.gene27271	0.803293	3.74E-49	-1.69E-46
MS.gene058146	MS.gene27271	0.833612	4.82E-56	-1.69E-46
MS.gene058595	MS.gene27271	0.816734	4.75E-52	-1.69E-46
MS.gene059128	MS.gene27271	0.830278	3.21E-55	-1.69E-46
MS.gene059161	MS.gene27271	0.838232	3.25E-57	-1.69E-46
MS.gene059609	MS.gene27271	0.836038	1.18E-56	-1.69E-46
MS.gene060011	MS.gene27271	0.842921	1.92E-58	-1.69E-46
MS.gene060246	MS.gene27271	0.802816	4.69E-49	-1.69E-46
MS.gene060322	MS.gene27271	0.856235	3.69E-62	-1.69E-46
MS.gene060495	MS.gene27271	0.811596	6.47E-51	-1.69E-46
MS.gene060683	MS.gene27271	0.831277	1.83E-55	-1.69E-46
MS.gene060685	MS.gene27271	0.826722	2.32E-54	-1.69E-46
MS.gene060802	MS.gene27271	0.85205	5.95E-61	-1.69E-46
MS.gene060803	MS.gene27271	0.830522	2.80E-55	-1.69E-46
MS.gene060940	MS.gene27271	0.812079	5.08E-51	-1.69E-46
MS.gene060942	MS.gene27271	0.838119	3.48E-57	-1.69E-46
MS.gene060943	MS.gene27271	0.818291	2.12E-52	-1.69E-46
MS.gene060998	MS.gene27271	0.860611	1.83E-63	-1.69E-46
MS.gene061002	MS.gene27271	0.823674	1.22E-53	-1.69E-46
MS.gene061114	MS.gene27271	-0.803162	3.98E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene56558	MS.gene27271	PPI
MS.gene27271	MS.gene02264	PPI
MS.gene96813	MS.gene27271	PPI
MS.gene27271	MS.gene01283	PPI
MS.gene01696	MS.gene27271	PPI
MS.gene006767	MS.gene27271	PPI
MS.gene031529	MS.gene27271	PPI
MS.gene69799	MS.gene27271	PPI
MS.gene27271	MS.gene002305	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27271.t1	MTR_1g023140	96.581	117	4	0	2	118	3	119	4.18e-78	226
MS.gene27271.t1	MTR_3g112410	80.909	110	21	0	2	111	3	112	2.74e-62	186
MS.gene27271.t1	MTR_5g021180	67.593	108	35	0	4	111	7	114	9.85e-53	162
MS.gene27271.t1	MTR_7g009070	50.980	102	50	0	11	112	32	133	6.15e-37	122
MS.gene27271.t1	MTR_8g107460	50.000	108	54	0	4	111	253	360	1.48e-33	119
MS.gene27271.t1	MTR_8g005320	40.566	106	63	0	3	108	24	129	3.94e-32	110
MS.gene27271.t1	MTR_8g006685	40.566	106	63	0	3	108	93	198	1.66e-31	110
MS.gene27271.t1	MTR_8g006560	40.566	106	63	0	3	108	93	198	1.66e-31	110
MS.gene27271.t1	MTR_7g073680	39.286	112	68	0	3	114	22	133	8.68e-30	104
MS.gene27271.t1	MTR_5g037930	44.954	109	60	0	5	113	20	128	1.52e-28	100
MS.gene27271.t1	MTR_5g037950	45.283	106	58	0	7	112	21	126	9.79e-27	96.3
MS.gene27271.t1	MTR_3g112400	52.475	101	28	3	1	101	1	81	1.92e-26	94.4
MS.gene27271.t1	MTR_2g010750	38.261	115	71	0	4	118	8	122	2.03e-25	92.8
MS.gene27271.t1	MTR_5g037890	42.202	109	63	0	5	113	18	126	3.86e-25	92.4
MS.gene27271.t1	MTR_2g082590	33.929	112	74	0	4	115	9	120	5.49e-21	81.6
MS.gene27271.t1	MTR_4g094690	38.298	94	56	1	8	99	62	155	1.65e-20	81.6
MS.gene27271.t1	MTR_4g081380	35.294	102	66	0	4	105	9	110	1.61e-19	77.8
MS.gene27271.t1	MTR_2g082590	39.080	87	53	0	29	115	47	133	3.72e-18	74.7
MS.gene27271.t1	MTR_1g098660	40.789	76	44	1	25	99	76	151	4.18e-18	75.5
MS.gene27271.t1	MTR_3g089065	35.780	109	65	3	8	114	70	175	5.97e-17	72.8
MS.gene27271.t1	MTR_6g092700	30.172	116	76	3	5	117	75	188	9.36e-16	70.1
MS.gene27271.t1	MTR_7g080250	38.095	105	61	2	5	108	75	176	1.51e-15	68.9
MS.gene27271.t1	MTR_7g102130	33.721	86	55	1	26	111	44	127	2.21e-14	64.7
MS.gene27271.t1	MTR_2g079420	39.474	76	45	1	25	99	84	159	5.30e-14	64.7
MS.gene27271.t1	MTR_3g435370	28.319	113	76	3	4	113	86	196	2.29e-13	64.7
MS.gene27271.t1	MTR_3g435370	28.319	113	76	3	4	113	86	196	2.78e-13	64.3
MS.gene27271.t1	MTR_8g059015	36.364	88	54	2	25	110	87	174	4.39e-13	62.4
MS.gene27271.t1	MTR_1g114290	36.842	95	48	4	26	114	85	173	6.68e-13	62.0
MS.gene27271.t1	MTR_2g010750	32.857	70	47	0	4	73	8	77	5.19e-12	57.8
MS.gene27271.t1	MTR_4g088905	31.132	106	69	1	5	110	3	104	1.28e-11	60.5
MS.gene27271.t1	MTR_2g079340	33.784	74	48	1	26	98	51	124	3.27e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27271.t1	AT3G51030	68.519	108	34	0	4	111	5	112	1.23e-55	169
MS.gene27271.t1	AT1G45145	58.559	111	46	0	1	111	1	111	6.92e-48	149
MS.gene27271.t1	AT5G42980	58.120	117	49	0	1	117	1	117	7.96e-47	147
MS.gene27271.t1	AT1G19730	57.265	117	49	1	2	117	3	119	4.49e-43	137
MS.gene27271.t1	AT3G17880	41.667	108	63	0	4	111	262	369	3.38e-31	114
MS.gene27271.t1	AT3G17880	41.667	108	63	0	4	111	269	376	4.28e-31	113
MS.gene27271.t1	AT5G39950	42.991	107	61	0	6	112	26	132	4.98e-31	107
MS.gene27271.t1	AT3G56420	41.346	104	61	0	2	105	37	140	1.09e-29	104
MS.gene27271.t1	AT3G56420	41.346	104	61	0	2	105	37	140	1.09e-29	104
MS.gene27271.t1	AT3G56420	41.346	104	61	0	2	105	37	140	1.09e-29	104
MS.gene27271.t1	AT3G08710	38.182	110	68	0	5	114	23	132	5.76e-29	102
MS.gene27271.t1	AT3G08710	38.182	110	68	0	5	114	23	132	5.76e-29	102
MS.gene27271.t1	AT3G08710	38.182	110	68	0	5	114	23	132	5.76e-29	102
MS.gene27271.t1	AT1G59730	39.286	112	68	0	1	112	17	128	1.55e-28	101
MS.gene27271.t1	AT1G69880	37.963	108	67	0	7	114	39	146	9.86e-27	97.1
MS.gene27271.t1	AT2G40790	40.196	102	61	0	4	105	39	140	1.72e-25	94.0
MS.gene27271.t1	AT2G40790	40.196	102	61	0	4	105	39	140	1.72e-25	94.0
MS.gene27271.t1	AT1G11530	39.604	101	61	0	5	105	2	102	7.82e-23	86.3
MS.gene27271.t1	AT2G35010	37.615	109	66	1	2	108	81	189	1.26e-20	82.8
MS.gene27271.t1	AT2G35010	37.615	109	66	1	2	108	81	189	1.26e-20	82.8
MS.gene27271.t1	AT1G43560	37.778	90	50	2	25	108	74	163	2.05e-17	73.6
MS.gene27271.t1	AT1G76760	39.474	76	45	1	25	99	79	154	6.98e-17	72.4
MS.gene27271.t1	AT4G26160	32.727	110	69	3	6	112	94	201	1.44e-16	72.8
MS.gene27271.t1	AT5G16400	41.463	82	47	1	28	108	98	179	4.17e-16	70.9
MS.gene27271.t1	AT1G31020	38.372	86	51	1	15	98	59	144	8.78e-16	69.3
MS.gene27271.t1	AT3G02730	38.636	88	53	1	28	114	88	175	1.24e-15	69.3
MS.gene27271.t1	AT4G32580	31.915	94	64	0	17	110	11	104	1.37e-12	60.8
MS.gene27271.t1	AT1G03680	30.088	113	72	4	3	111	69	178	1.40e-12	61.2
MS.gene27271.t1	AT3G15360	34.940	83	52	2	30	110	107	189	1.56e-12	61.6
MS.gene27271.t1	AT3G53220	31.395	86	57	1	26	111	42	125	9.14e-12	58.2
MS.gene27271.t1	AT4G29670	26.364	110	76	3	6	112	104	211	1.20e-11	59.7
MS.gene27271.t1	AT4G29670	27.711	83	58	1	6	88	104	184	2.17e-11	58.9
MS.gene27271.t1	AT4G04950	31.915	94	64	0	17	110	11	104	2.57e-11	59.7
MS.gene27271.t1	AT4G03520	32.530	83	54	2	30	110	101	183	4.34e-11	57.4

Find 32 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGACTTCAGGAGTCTTCTT+AGG	0.296484	1.1:-1514477	None:intergenic
TTAGGAATCTCAGCTAGAAT+TGG	0.349137	1.1:-1514459	None:intergenic
AGAAGGTAAGGAAGTGGATA+AGG	0.412033	1.1:+1514916	MS.gene27271:CDS
CTTATTCTTGAAAGAAGGTA+AGG	0.442943	1.1:+1514904	MS.gene27271:CDS
ACCTTCTTATTCTTGAAAGA+AGG	0.451509	1.1:+1514899	MS.gene27271:CDS
ACCTTGAGGAAGATGACTTC+AGG	0.453976	1.1:-1514489	None:intergenic
AAATGCAATCACCAAGCATA+AGG	0.465102	1.1:+1514967	MS.gene27271:CDS
TAAGGTAGTTGGTGCTAGGA+AGG	0.479587	1.1:+1514934	MS.gene27271:CDS
TGGATAAGGTAGTTGGTGCT+AGG	0.488936	1.1:+1514930	MS.gene27271:CDS
AAGGAAGTGGATAAGGTAGT+TGG	0.491604	1.1:+1514923	MS.gene27271:CDS
AACAGTTGCATCCTTATGCT+TGG	0.492616	1.1:-1514978	None:intergenic
ACCTTCTTTCAAGAATAAGA+AGG	0.495256	1.1:-1514900	None:intergenic
TTCAGAGTGTTGCTGAGGAA+TGG	0.506641	1.1:+1514858	MS.gene27271:intron
GGTGGATATTGATGAAGTAA+AGG	0.509236	1.1:+1514509	MS.gene27271:CDS
CTAGAATTGGAGCAATAAAA+CGG	0.524722	1.1:-1514446	None:intergenic
CTTGAAAGAAGGTAAGGAAG+TGG	0.537499	1.1:+1514910	MS.gene27271:CDS
TGGTGCTAGGAAGGAAGAGC+TGG	0.539272	1.1:+1514943	MS.gene27271:CDS
TCTTTCAAGAATAAGAAGGT+TGG	0.555397	1.1:-1514896	None:intergenic
TCCTGAAGTCATCTTCCTCA+AGG	0.561588	1.1:+1514488	MS.gene27271:CDS
CTCCTCCTTCCACTGTTCAA+CGG	0.563671	1.1:-1512846	None:intergenic
CATTGAATAATAGATTGTGG+TGG	0.597012	1.1:+1514395	MS.gene27271:intron
GCAGCTGAAGGACAGGTGAT+CGG	0.598988	1.1:+1512815	MS.gene27271:CDS
GTGGAAGGAGGAGATCCAGA+AGG	0.606920	1.1:+1512856	MS.gene27271:CDS
TGGAAGGAGGAGATCCAGAA+GGG	0.610530	1.1:+1512857	MS.gene27271:CDS
TGCTGAGGAATGGTCTGTTG+AGG	0.618156	1.1:+1514868	MS.gene27271:CDS
CTTCATCAATATCCACCTTG+AGG	0.619089	1.1:-1514503	None:intergenic
TCATACCGTTGAACAGTGGA+AGG	0.620633	1.1:+1512841	MS.gene27271:CDS
ATTGGAGCAATAAAACGGCA+TGG	0.627618	1.1:-1514441	None:intergenic
CCTATTTCAGAGTGTTGCTG+AGG	0.630806	1.1:+1514853	MS.gene27271:intron
TACCGTTGAACAGTGGAAGG+AGG	0.648899	1.1:+1512844	MS.gene27271:CDS
TGAAGTCATCTTCCTCAAGG+TGG	0.658466	1.1:+1514491	MS.gene27271:CDS
GTGTTCATACCGTTGAACAG+TGG	0.666984	1.1:+1512837	MS.gene27271:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAATAAACTCTAAACAA+GGG	+	chr1.1:1513933-1513952	MS.gene27271:intron	15.0%
!!	TTAAATTAAGCATTTAATGT+AGG	-	chr1.1:1513986-1514005	None:intergenic	15.0%
!!!	ACAATTTTGTTTTTCATTAT+TGG	-	chr1.1:1513472-1513491	None:intergenic	15.0%
!!!	ATGTATATTTTAGTGTATTT+GGG	+	chr1.1:1513137-1513156	MS.gene27271:intron	15.0%
!!!	ATGTTTCTAATATTTTAAGA+TGG	+	chr1.1:1514765-1514784	MS.gene27271:intron	15.0%
!!	ATAAAGGTGGTTATAAATTA+AGG	-	chr1.1:1514246-1514265	None:intergenic	20.0%
!!	ATTAAACTTGAGAAAGAATA+AGG	-	chr1.1:1514560-1514579	None:intergenic	20.0%
!!	CAAATTACTTATATATACTC+TGG	-	chr1.1:1513538-1513557	None:intergenic	20.0%
!!	CAGAGTATATATAAGTAATT+TGG	+	chr1.1:1513536-1513555	MS.gene27271:intron	20.0%
!!	CATAAAATAAACTCTAAACA+AGG	+	chr1.1:1513932-1513951	MS.gene27271:intron	20.0%
!!	GTTTGTGATGATATATTATA+TGG	+	chr1.1:1513260-1513279	MS.gene27271:intron	20.0%
!!	TGTTAAAAACCTAATTATAG+TGG	+	chr1.1:1513294-1513313	MS.gene27271:intron	20.0%
!!	TTCTTCGAGTATTATATTAT+AGG	-	chr1.1:1513824-1513843	None:intergenic	20.0%
!!	TTTCATTGAATAATAGATTG+TGG	+	chr1.1:1514392-1514411	MS.gene27271:intron	20.0%
!!!	ACTTTTGGATCAAAATATAT+AGG	-	chr1.1:1513038-1513057	None:intergenic	20.0%
!!!	CAACAAAGAATTTATTTTGT+TGG	+	chr1.1:1514175-1514194	MS.gene27271:intron	20.0%
!!!	CATGTATATTTTAGTGTATT+TGG	+	chr1.1:1513136-1513155	MS.gene27271:intron	20.0%
!!!	CTTGTTTAGAGTTTATTTTA+TGG	-	chr1.1:1513934-1513953	None:intergenic	20.0%
!!!	CTTTTGGATCAAAATATATA+GGG	-	chr1.1:1513037-1513056	None:intergenic	20.0%
!	AAAAAAACTAACCTAGAGAA+TGG	-	chr1.1:1513961-1513980	None:intergenic	25.0%
!	AAATTACTAAGTTAGTGTCA+CGG	-	chr1.1:1514130-1514149	None:intergenic	25.0%
!	AGGCTAATAATAACATTGAA+AGG	-	chr1.1:1514101-1514120	None:intergenic	25.0%
!	ATGATTATGACATTCATATG+TGG	-	chr1.1:1513882-1513901	None:intergenic	25.0%
!	CAAAACAAATAAGCACATAA+AGG	-	chr1.1:1514262-1514281	None:intergenic	25.0%
!!	CTTTATGTGCTTATTTGTTT+TGG	+	chr1.1:1514260-1514279	MS.gene27271:intron	25.0%
!!	GAACAATTCATAAACACTTT+TGG	-	chr1.1:1513053-1513072	None:intergenic	25.0%
!!	TATTTTATGGCACAAGTATT+TGG	-	chr1.1:1513921-1513940	None:intergenic	25.0%
!!!	TATTTTGACCATACTTTAGT+AGG	-	chr1.1:1513323-1513342	None:intergenic	25.0%
!!!	TTGCCATTTTTATATATGCA+TGG	+	chr1.1:1514047-1514066	MS.gene27271:intron	25.0%
!!!	TTTGATGAATACCAGTTTTT+TGG	-	chr1.1:1512891-1512910	None:intergenic	25.0%
	AACAAATAAGCACATAAAGG+TGG	-	chr1.1:1514259-1514278	None:intergenic	30.0%
	ACCTTCTTATTCTTGAAAGA+AGG	+	chr1.1:1514899-1514918	MS.gene27271:CDS	30.0%
	ACCTTCTTTCAAGAATAAGA+AGG	-	chr1.1:1514903-1514922	None:intergenic	30.0%
	AGTAGGAAACCACTATAATT+AGG	-	chr1.1:1513306-1513325	None:intergenic	30.0%
	CATTGAATAATAGATTGTGG+TGG	+	chr1.1:1514395-1514414	MS.gene27271:intron	30.0%
	CTAGAATTGGAGCAATAAAA+CGG	-	chr1.1:1514449-1514468	None:intergenic	30.0%
	CTTATTCTTGAAAGAAGGTA+AGG	+	chr1.1:1514904-1514923	MS.gene27271:CDS	30.0%
	GATCATAAGATGACATAACA+TGG	-	chr1.1:1514720-1514739	None:intergenic	30.0%
	GGACCATGCATATATAAAAA+TGG	-	chr1.1:1514053-1514072	None:intergenic	30.0%
	TAATTTAACCGTCAAACCTA+TGG	+	chr1.1:1513341-1513360	MS.gene27271:intron	30.0%
	TCTTTCAAGAATAAGAAGGT+TGG	-	chr1.1:1514899-1514918	None:intergenic	30.0%
!	TAATTAGGTTTTTAACACCG+TGG	-	chr1.1:1513291-1513310	None:intergenic	30.0%
!	TGTAGTTTTTGTCAAAATCG+TGG	+	chr1.1:1514640-1514659	MS.gene27271:intron	30.0%
!	TTTTTATATATGCATGGTCC+AGG	+	chr1.1:1514053-1514072	MS.gene27271:intron	30.0%
!!	CACAGTTGGCAAATTTTAAT+TGG	-	chr1.1:1513756-1513775	None:intergenic	30.0%
!!!	AGAATTTTTCAAGACACAGT+TGG	-	chr1.1:1513770-1513789	None:intergenic	30.0%
	AAATGCAATCACCAAGCATA+AGG	+	chr1.1:1514967-1514986	MS.gene27271:CDS	35.0%
	AGTGGTTTCCTACTAAAGTA+TGG	+	chr1.1:1513312-1513331	MS.gene27271:intron	35.0%
	CTATTTGCCATAATTCAAGG+AGG	+	chr1.1:1513694-1513713	MS.gene27271:intron	35.0%
	GCACTATTTGCCATAATTCA+AGG	+	chr1.1:1513691-1513710	MS.gene27271:intron	35.0%
	GGTGGATATTGATGAAGTAA+AGG	+	chr1.1:1514509-1514528	MS.gene27271:CDS	35.0%
	TATATTATATGGAGCCACCA+CGG	+	chr1.1:1513271-1513290	MS.gene27271:intron	35.0%
	TTAGGAATCTCAGCTAGAAT+TGG	-	chr1.1:1514462-1514481	None:intergenic	35.0%
!!	AACTAGTCTTAAAGCCGATT+CGG	+	chr1.1:1514830-1514849	MS.gene27271:intron	35.0%
!!	AGAGGAGATTAGTCTCATAA+CGG	+	chr1.1:1513225-1513244	MS.gene27271:intron	35.0%
!!	GATTAGTCTCATAACGGATT+TGG	+	chr1.1:1513231-1513250	MS.gene27271:intron	35.0%
	AACAGTTGCATCCTTATGCT+TGG	-	chr1.1:1514981-1515000	None:intergenic	40.0%
	AAGGAAGTGGATAAGGTAGT+TGG	+	chr1.1:1514923-1514942	MS.gene27271:CDS	40.0%
	AATATTGCCCTAGATCATCG+TGG	+	chr1.1:1513089-1513108	MS.gene27271:intron	40.0%
	AGAAGGTAAGGAAGTGGATA+AGG	+	chr1.1:1514916-1514935	MS.gene27271:CDS	40.0%
	ATGACTTCAGGAGTCTTCTT+AGG	-	chr1.1:1514480-1514499	None:intergenic	40.0%
	ATTGGAGCAATAAAACGGCA+TGG	-	chr1.1:1514444-1514463	None:intergenic	40.0%
	CTTCATCAATATCCACCTTG+AGG	-	chr1.1:1514506-1514525	None:intergenic	40.0%
	CTTGAAAGAAGGTAAGGAAG+TGG	+	chr1.1:1514910-1514929	MS.gene27271:CDS	40.0%
	GGCAAATAGTGCATTGTGAT+TGG	-	chr1.1:1513683-1513702	None:intergenic	40.0%
	GGGAAATGACTCCAAAAAAC+TGG	+	chr1.1:1512877-1512896	MS.gene27271:CDS	40.0%
	TGTGGCTGAAAAATAGGAAG+TGG	+	chr1.1:1513717-1513736	MS.gene27271:intron	40.0%
	TTAGACCGCAACTTAAAGCT+CGG	-	chr1.1:1513184-1513203	None:intergenic	40.0%
!	GATTTTACTGCTTCATGGTG+TGG	+	chr1.1:1514417-1514436	MS.gene27271:CDS	40.0%
!	TTAGGTTTTTAACACCGTGG+TGG	-	chr1.1:1513288-1513307	None:intergenic	40.0%
!!	AAACAAGGGTACCATTCTCT+AGG	+	chr1.1:1513947-1513966	MS.gene27271:intron	40.0%
!!	AGTTAGTGTCACGGTTTTAG+AGG	-	chr1.1:1514121-1514140	None:intergenic	40.0%
!!!	TGGTGGATTTTACTGCTTCA+TGG	+	chr1.1:1514412-1514431	MS.gene27271:CDS	40.0%
!!!	TTTTGGAGTCATTTCCCTTC+TGG	-	chr1.1:1512874-1512893	None:intergenic	40.0%
	AACACTGTCCACGATGATCT+AGG	-	chr1.1:1513100-1513119	None:intergenic	45.0%
	ACACTGTCCACGATGATCTA+GGG	-	chr1.1:1513099-1513118	None:intergenic	45.0%
	ACCTTGAGGAAGATGACTTC+AGG	-	chr1.1:1514492-1514511	None:intergenic	45.0%
	AGCCACACCTCCTTGAATTA+TGG	-	chr1.1:1513704-1513723	None:intergenic	45.0%
	CTTGTGCACCATAGGTTTGA+CGG	-	chr1.1:1513352-1513371	None:intergenic	45.0%
	GTGTTCATACCGTTGAACAG+TGG	+	chr1.1:1512837-1512856	MS.gene27271:CDS	45.0%
	TAACGCAAGTTCGTTCATCC+TGG	-	chr1.1:1514074-1514093	None:intergenic	45.0%
	TAATCTCCTCTCGTGTGCTT+AGG	-	chr1.1:1513216-1513235	None:intergenic	45.0%
	TAGACCGCAACTTAAAGCTC+GGG	-	chr1.1:1513183-1513202	None:intergenic	45.0%
	TCATACCGTTGAACAGTGGA+AGG	+	chr1.1:1512841-1512860	MS.gene27271:CDS	45.0%
	TCCTGAAGTCATCTTCCTCA+AGG	+	chr1.1:1514488-1514507	MS.gene27271:CDS	45.0%
	TGAAGTCATCTTCCTCAAGG+TGG	+	chr1.1:1514491-1514510	MS.gene27271:CDS	45.0%
	TGCCATAATTCAAGGAGGTG+TGG	+	chr1.1:1513699-1513718	MS.gene27271:intron	45.0%
	TTGTCTCACTTGTGCACCAT+AGG	-	chr1.1:1513360-1513379	None:intergenic	45.0%
!	AGGAGGTGTGGCTGAAAAAT+AGG	+	chr1.1:1513711-1513730	MS.gene27271:intron	45.0%
!	TGGATAAGGTAGTTGGTGCT+AGG	+	chr1.1:1514930-1514949	MS.gene27271:CDS	45.0%
!!	ATCACCCGAGCTTTAAGTTG+CGG	+	chr1.1:1513176-1513195	MS.gene27271:intron	45.0%
!!	CACTCTGAAATAGGCCGAAT+CGG	-	chr1.1:1514847-1514866	None:intergenic	45.0%
!!	CCTATTTCAGAGTGTTGCTG+AGG	+	chr1.1:1514853-1514872	MS.gene27271:intron	45.0%
!!	CCTCAGCAACACTCTGAAAT+AGG	-	chr1.1:1514856-1514875	None:intergenic	45.0%
!!	TAAGGTAGTTGGTGCTAGGA+AGG	+	chr1.1:1514934-1514953	MS.gene27271:CDS	45.0%
!!	TTCAGAGTGTTGCTGAGGAA+TGG	+	chr1.1:1514858-1514877	MS.gene27271:intron	45.0%
	CTCCTCCTTCCACTGTTCAA+CGG	-	chr1.1:1512849-1512868	None:intergenic	50.0%
	TACCGTTGAACAGTGGAAGG+AGG	+	chr1.1:1512844-1512863	MS.gene27271:CDS	50.0%
	TCCTCTCGTGTGCTTAGGTT+CGG	-	chr1.1:1513211-1513230	None:intergenic	50.0%
	TGCTGAGGAATGGTCTGTTG+AGG	+	chr1.1:1514868-1514887	MS.gene27271:CDS	50.0%
	TGGAAGGAGGAGATCCAGAA+GGG	+	chr1.1:1512857-1512876	MS.gene27271:CDS	50.0%
	GCAGCTGAAGGACAGGTGAT+CGG	+	chr1.1:1512815-1512834	MS.gene27271:CDS	55.0%
	GTGGAAGGAGGAGATCCAGA+AGG	+	chr1.1:1512856-1512875	MS.gene27271:CDS	55.0%
	TCCGAACCTAAGCACACGAG+AGG	+	chr1.1:1513207-1513226	MS.gene27271:intron	55.0%
!	TGGTGCTAGGAAGGAAGAGC+TGG	+	chr1.1:1514943-1514962	MS.gene27271:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	1512812	1515012	1512812	ID=MS.gene27271
chr1.1	mRNA	1512812	1515012	1512812	ID=MS.gene27271.t1;Parent=MS.gene27271
chr1.1	exon	1512812	1512898	1512812	ID=MS.gene27271.t1.exon1;Parent=MS.gene27271.t1
chr1.1	CDS	1512812	1512898	1512812	ID=cds.MS.gene27271.t1;Parent=MS.gene27271.t1
chr1.1	exon	1514408	1514530	1514408	ID=MS.gene27271.t1.exon2;Parent=MS.gene27271.t1
chr1.1	CDS	1514408	1514530	1514408	ID=cds.MS.gene27271.t1;Parent=MS.gene27271.t1
chr1.1	exon	1514863	1515012	1514863	ID=MS.gene27271.t1.exon3;Parent=MS.gene27271.t1
chr1.1	CDS	1514863	1515012	1514863	ID=cds.MS.gene27271.t1;Parent=MS.gene27271.t1

Gene Sequence

>MS.gene27271

ATGGCAGCTGAAGGACAGGTGATCGGTGTTCATACCGTTGAACAGTGGAAGGAGGAGATCCAGAAGGGAAATGACTCCAAAAAACTGGTATTCATCAAATACTATCTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAACTTTACTTTCAATCTTCTTCATATATATTTGCTTTTCGACCCTATATATTTTGATCCAAAAGTGTTTATGAATTGTTCAATATTCTTGTCTGAAAATATTGCCCTAGATCATCGTGGACAGTGTTAAGTTTTGTATAGTTACATGTATATTTTAGTGTATTTGGGTTAACAATTTAAGTTAATCACCCGAGCTTTAAGTTGCGGTCTAAGCATCCGAACCTAAGCACACGAGAGGAGATTAGTCTCATAACGGATTTGGACTTAAGTTTGTGATGATATATTATATGGAGCCACCACGGTGTTAAAAACCTAATTATAGTGGTTTCCTACTAAAGTATGGTCAAAATAATTTAACCGTCAAACCTATGGTGCACAAGTGAGACAAGTTTTACATCATGTATACAAACTTGTTTTTACTATTAGATCAAAAAGTCTTACTATTAAATCAAATGAGATGTGATTGAAATTATTGATCCAATAATGAAAAACAAAATTGTACAAGTAGCAACACTAATTTACATGTTTTCATTAGATAAAACTCCAGAGTATATATAAGTAATTTGGAAGATGAAATATGATTAAACTATTTGAATATAAATTGTAATCAGTTTTCATAATATAATATAAGAGCAATGCTATATCTCTAGAAAAATAACATCAAGAATTCTTCAAGAATAAAGAGTAGCCAATCACAATGCACTATTTGCCATAATTCAAGGAGGTGTGGCTGAAAAATAGGAAGTGGACAAATAAATCATCCAATTAAAATTTGCCAACTGTGTCTTGAAAAATTCTTGAAGCTATTTTTCTTGAGAAGTAGCATTTTCCTATAATATAATACTCGAAGAACTATTTGAAATTAGCTAGAAATAATGAGCTGAAAACCACATATGAATGTCATAATCATATTTTTATAAGTTCATCCAAATACTTGTGCCATAAAATAAACTCTAAACAAGGGTACCATTCTCTAGGTTAGTTTTTTTAACCTACATTAAATGCTTAATTTAAAATCAAATTTTTGTATCGTTGTGTTTTGAACTAAGCATAATTTGCCATTTTTATATATGCATGGTCCAGGATGAACGAACTTGCGTTACGTACCTTTCAATGTTATTATTAGCCTCTAAAACCGTGACACTAACTTAGTAATTTACTTTTTTATTTGTAGAGTTAACATCAACAAAGAATTTATTTTGTTGGTTGAAATGACAGTTGTGACATTTCAAAATGTCATTTTCATGTTTGCCTTAATTTATAACCACCTTTATGTGCTTATTTGTTTTGGAAACTTTATGCATTTAAATTATTTGAATATTTTTTGAGTTTAATTTTTTTTTTTTTGTTGCTGTTTAAGTTGATAATAGCATTATTCATAACACTTATAATGAATTGCATTTCATTGAATAATAGATTGTGGTGGATTTTACTGCTTCATGGTGTGGTCCATGCCGTTTTATTGCTCCAATTCTAGCTGAGATTCCTAAGAAGACTCCTGAAGTCATCTTCCTCAAGGTGGATATTGATGAAGTAAAGGTATAAGCATATCTTAATCTATATTACCTTATTCTTTCTCAAGTTTAATACATGTTTAGAATCACGTTGAGTTTGAAAAAATCACAACATGATTTTTGTAGCTCTATAGTGTAGTTTTTGTCAAAATCGTGGTAGCACGTAGTGATTCTTGATTCTGTCAAACTCAGATAACACAATCAAAGTTTACCATGTTATGTCATCTTATGATCATACTATAGAAACATTGTTTGAAACATGTTTCTAATATTTTAAGATGGTGACTGATTAATTACTATATAGTTAAACTTATTTTTACTTCAAACTAGTCTTAAAGCCGATTCGGCCTATTTCAGAGTGTTGCTGAGGAATGGTCTGTTGAGGCTATGCCAACCTTCTTATTCTTGAAAGAAGGTAAGGAAGTGGATAAGGTAGTTGGTGCTAGGAAGGAAGAGCTGGAAAATGCAATCACCAAGCATAAGGATGCAACTGTTGCTACTACTTGA

Protein sequence

>MS.gene27271.t1

MAAEGQVIGVHTVEQWKEEIQKGNDSKKLIVVDFTASWCGPCRFIAPILAEIPKKTPEVIFLKVDIDEVKSVAEEWSVEAMPTFLFLKEGKEVDKVVGARKEELENAITKHKDATVATT