AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37570.t1 MTR_5g089030 96.907 97 3 0 1 97 24 120 4.76e-64 189
MS.gene37570.t1 MTR_5g089030 96.907 97 3 0 1 97 17 113 6.23e-64 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37570.t1 AT5G12240 51.316 76 34 3 23 96 40 114 2.88e-16 68.6

Find 17 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAACTAACAGATTCATTT+TGG 0.150883 5.2:-75572606 MS.gene37570:intron
TTTAGATGTTGCTCGAAAAT+TGG 0.248254 5.2:-75571273 MS.gene37570:CDS
AAAGTCAGAGGCTGCTAAAA+AGG 0.332380 5.2:-75572575 MS.gene37570:intron
AGATCCAGGTGTTAATTGTC+TGG 0.439476 5.2:+75572930 None:intergenic
TTAACACCTGGATCTGGATC+TGG 0.464092 5.2:-75572922 MS.gene37570:CDS
AGACAATTAACACCTGGATC+TGG 0.479178 5.2:-75572928 MS.gene37570:CDS
GCAGATCCAGATCCAGATCC+AGG 0.505288 5.2:+75572916 None:intergenic
TGCATGGAATGAAAAGGATA+AGG 0.508203 5.2:-75572789 MS.gene37570:CDS
TATCACTGCAGCTAAATGAA+TGG 0.519982 5.2:-75572817 MS.gene37570:intron
ATCTGGATCTGCACAGGAAG+AGG 0.529978 5.2:-75572905 MS.gene37570:intron
ATCTGGATCTGGATCTGCAC+AGG 0.538020 5.2:-75572911 MS.gene37570:CDS
GCATGATGCATGGAATGAAA+AGG 0.553411 5.2:-75572795 MS.gene37570:CDS
TTGGAACAAGCTAAAGTCAG+AGG 0.617833 5.2:-75572587 MS.gene37570:CDS
ACAGGTTGGTACAGAAGAAG+CGG 0.620709 5.2:-75571704 MS.gene37570:intron
TAAATGAATGGCATGATGCA+TGG 0.637004 5.2:-75572805 MS.gene37570:CDS
TCATCCAGACAATTAACACC+TGG 0.646730 5.2:-75572934 MS.gene37570:CDS
AATCAAGGAATCAACAAACA+TGG 0.667146 5.2:-75572758 MS.gene37570:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAAATGAATAAAATTGA+TGG - chr5.2:75572815-75572834 MS.gene37570:intron 10.0%
!! TTGTTCTTAATTATTAAAAA+TGG - chr5.2:75571718-75571737 MS.gene37570:intron 10.0%
!!! ATTTTTAATAATTAAGAACA+AGG + chr5.2:75571719-75571738 None:intergenic 10.0%
!!! TAAAACAATAAAAGAATTTT+TGG + chr5.2:75572892-75572911 None:intergenic 10.0%
!!! TTTTATTCATTTATATTTTC+TGG + chr5.2:75572811-75572830 None:intergenic 10.0%
!!! TTTTATTGTTTTATTTCTTT+AGG - chr5.2:75572899-75572918 MS.gene37570:intron 10.0%
!!! AAATGAACATTTTGTTTTTT+AGG - chr5.2:75572682-75572701 MS.gene37570:intron 15.0%
!!! AATGAACATTTTGTTTTTTA+GGG - chr5.2:75572683-75572702 MS.gene37570:intron 15.0%
!!! GTTTAAATGATTATCATTTT+GGG - chr5.2:75572575-75572594 MS.gene37570:intron 15.0%
!! AAAATCAAAGTAATTCAAAC+TGG + chr5.2:75571960-75571979 None:intergenic 20.0%
!! AAATCAAAGTAATTCAAACT+GGG + chr5.2:75571959-75571978 None:intergenic 20.0%
!! ATAATTAAGAACAAGGATAA+TGG + chr5.2:75571712-75571731 None:intergenic 20.0%
!! ATGTAAAGAAAAAGTAAACA+AGG + chr5.2:75571781-75571800 None:intergenic 20.0%
!! ATTTATAGAAAGTAGTTTCT+TGG - chr5.2:75572637-75572656 MS.gene37570:intron 20.0%
!! TGTAAAGAAAAAGTAAACAA+GGG + chr5.2:75571780-75571799 None:intergenic 20.0%
!! TTGAAAGTTTCATTTAATGA+TGG - chr5.2:75572161-75572180 MS.gene37570:intron 20.0%
!!! ATGAACATTTTGTTTTTTAG+GGG - chr5.2:75572684-75572703 MS.gene37570:intron 20.0%
!!! CGTTTAAATGATTATCATTT+TGG - chr5.2:75572574-75572593 MS.gene37570:intron 20.0%
!!! TTATATTTTCTGGAATTTCA+TGG + chr5.2:75572801-75572820 None:intergenic 20.0%
!!! TTCTTTCATAGAAAGTATTA+TGG - chr5.2:75572600-75572619 MS.gene37570:intron 20.0%
!!! TTGATGATTTTGTAAAGATA+GGG - chr5.2:75572193-75572212 MS.gene37570:intron 20.0%
! AACATAGCACATGTTTATAT+TGG - chr5.2:75571741-75571760 MS.gene37570:intron 25.0%
! ATTGGTTATAATGTTTCAAC+AGG - chr5.2:75572489-75572508 MS.gene37570:intron 25.0%
!! AAAATGAATCTGTTAGTTTG+GGG + chr5.2:75571606-75571625 None:intergenic 25.0%
!! AAAGAAGTCTTTCATTGATT+AGG - chr5.2:75572715-75572734 MS.gene37570:intron 25.0%
!! ATTTTGTTACAACAATCAAC+TGG - chr5.2:75571542-75571561 MS.gene37570:intron 25.0%
!! CAAAATGAATCTGTTAGTTT+GGG + chr5.2:75571607-75571626 None:intergenic 25.0%
!! GATGTATTTGTTGAACAATA+AGG + chr5.2:75571491-75571510 None:intergenic 25.0%
!! TGATGATTTTGTAAAGATAG+GGG - chr5.2:75572194-75572213 MS.gene37570:intron 25.0%
!!! GTTGATGATTTTGTAAAGAT+AGG - chr5.2:75572192-75572211 MS.gene37570:intron 25.0%
AAAGTAATTCAAACTGGGTT+TGG + chr5.2:75571954-75571973 None:intergenic 30.0%
AAATTGTCACACGCATAAAT+AGG - chr5.2:75572327-75572346 MS.gene37570:intron 30.0%
AATCAAGGAATCAACAAACA+TGG - chr5.2:75571453-75571472 MS.gene37570:intron 30.0%
AATTGTCACACGCATAAATA+GGG - chr5.2:75572328-75572347 MS.gene37570:intron 30.0%
ATAAAAATTCTTGTCCCATC+TGG - chr5.2:75572048-75572067 MS.gene37570:intron 30.0%
ATTTATGCGTGTGACAATTT+AGG + chr5.2:75572327-75572346 None:intergenic 30.0%
CCAAAATGAATCTGTTAGTT+TGG + chr5.2:75571608-75571627 None:intergenic 30.0%
GCTTTAACCTTAAAAGTTTG+AGG - chr5.2:75571833-75571852 MS.gene37570:intron 30.0%
GTATGCATGTAAAAATAAGC+AGG + chr5.2:75571675-75571694 None:intergenic 30.0%
GTTATAATGTTTCAACAGGT+TGG - chr5.2:75572493-75572512 MS.gene37570:intron 30.0%
TCTCTTTATCATGTTTGTGA+AGG - chr5.2:75572291-75572310 MS.gene37570:intron 30.0%
TTTAGATGTTGCTCGAAAAT+TGG - chr5.2:75572938-75572957 MS.gene37570:CDS 30.0%
! CCAAACTAACAGATTCATTT+TGG - chr5.2:75571605-75571624 MS.gene37570:intron 30.0%
! TAATCAGCCTCAAACTTTTA+AGG + chr5.2:75571843-75571862 None:intergenic 30.0%
!! GATAAGGATTTTGAGAATCA+AGG - chr5.2:75571438-75571457 MS.gene37570:intron 30.0%
!!! ATTTTTTTCACATGCCAGAT+GGG + chr5.2:75572065-75572084 None:intergenic 30.0%
AGGGATAAAAGATAAGTGAG+AGG - chr5.2:75572347-75572366 MS.gene37570:intron 35.0%
GGATAAAAGATAAGTGAGAG+GGG - chr5.2:75572349-75572368 MS.gene37570:intron 35.0%
GGGATAAAAGATAAGTGAGA+GGG - chr5.2:75572348-75572367 MS.gene37570:intron 35.0%
TAAATGAATGGCATGATGCA+TGG - chr5.2:75571406-75571425 MS.gene37570:intron 35.0%
TATCACTGCAGCTAAATGAA+TGG - chr5.2:75571394-75571413 MS.gene37570:intron 35.0%
TGCATGGAATGAAAAGGATA+AGG - chr5.2:75571422-75571441 MS.gene37570:intron 35.0%
!!! CATTTTTTTCACATGCCAGA+TGG + chr5.2:75572066-75572085 None:intergenic 35.0%
AAAGTCAGAGGCTGCTAAAA+AGG - chr5.2:75571636-75571655 MS.gene37570:intron 40.0%
AGACAATTAACACCTGGATC+TGG - chr5.2:75571283-75571302 MS.gene37570:CDS 40.0%
AGATCCAGGTGTTAATTGTC+TGG + chr5.2:75571284-75571303 None:intergenic 40.0%
GCATGATGCATGGAATGAAA+AGG - chr5.2:75571416-75571435 MS.gene37570:intron 40.0%
GGTTTCAAAGGAAAGGACTA+AGG + chr5.2:75572110-75572129 None:intergenic 40.0%
TAGAATGACACTACGCTCAT+TGG - chr5.2:75572471-75572490 MS.gene37570:intron 40.0%
TCATCCAGACAATTAACACC+TGG - chr5.2:75571277-75571296 MS.gene37570:CDS 40.0%
TTCAAAGGAAAGGACTAAGG+AGG + chr5.2:75572107-75572126 None:intergenic 40.0%
TTGGAACAAGCTAAAGTCAG+AGG - chr5.2:75571624-75571643 MS.gene37570:intron 40.0%
! AGAAGCGGAAAGATTTTGCA+AGG - chr5.2:75572522-75572541 MS.gene37570:intron 40.0%
! ATAGGGGAGATTGTTGTTGT+AGG - chr5.2:75572210-75572229 MS.gene37570:intron 40.0%
! CTAGGGTGTTATAACTGTGA+TGG - chr5.2:75572387-75572406 MS.gene37570:intron 40.0%
TGCATGCAAACCAAGTCTAC+TGG + chr5.2:75572138-75572157 None:intergenic 45.0%
TTAACACCTGGATCTGGATC+TGG - chr5.2:75571289-75571308 MS.gene37570:CDS 45.0%
!! ACAGGTTGGTACAGAAGAAG+CGG - chr5.2:75572507-75572526 MS.gene37570:intron 45.0%
!! GTCCTTTCCTTTGAAACCAG+TGG - chr5.2:75572112-75572131 MS.gene37570:intron 45.0%
AAACCAAGTCTACTGGCCAC+TGG + chr5.2:75572131-75572150 None:intergenic 50.0%
AAACCAGTGGCCAGTAGACT+TGG - chr5.2:75572125-75572144 MS.gene37570:intron 50.0%
AGGAAAGGACTAAGGAGGCA+AGG + chr5.2:75572102-75572121 None:intergenic 50.0%
ATCTGGATCTGCACAGGAAG+AGG - chr5.2:75571306-75571325 MS.gene37570:intron 50.0%
ATCTGGATCTGGATCTGCAC+AGG - chr5.2:75571300-75571319 MS.gene37570:intron 50.0%
GGTACATGTGTGTTGGGCTA+GGG - chr5.2:75572370-75572389 MS.gene37570:intron 50.0%
TGCACAGGAAGAGGTAGTTG+TGG - chr5.2:75571315-75571334 MS.gene37570:intron 50.0%
! CTGTGATGGTGATGTTGCAG+AGG - chr5.2:75572401-75572420 MS.gene37570:intron 50.0%
! GAGAGGGGTACATGTGTGTT+GGG - chr5.2:75572364-75572383 MS.gene37570:intron 50.0%
! TGAGAGGGGTACATGTGTGT+TGG - chr5.2:75572363-75572382 MS.gene37570:intron 50.0%
!! CTACTGGCCACTGGTTTCAA+AGG + chr5.2:75572122-75572141 None:intergenic 50.0%
!! GGCCACTGGTTTCAAAGGAA+AGG + chr5.2:75572117-75572136 None:intergenic 50.0%
GCAGATCCAGATCCAGATCC+AGG + chr5.2:75571298-75571317 None:intergenic 55.0%
GGGTACATGTGTGTTGGGCT+AGG - chr5.2:75572369-75572388 MS.gene37570:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 75571259 75572974 75571259 ID=MS.gene37570
chr5.2 mRNA 75571259 75572974 75571259 ID=MS.gene37570.t1;Parent=MS.gene37570
chr5.2 exon 75572906 75572974 75572906 ID=MS.gene37570.t1.exon1;Parent=MS.gene37570.t1
chr5.2 CDS 75572906 75572974 75572906 ID=cds.MS.gene37570.t1;Parent=MS.gene37570.t1
chr5.2 exon 75572759 75572828 75572759 ID=MS.gene37570.t1.exon2;Parent=MS.gene37570.t1
chr5.2 CDS 75572759 75572828 75572759 ID=cds.MS.gene37570.t1;Parent=MS.gene37570.t1
chr5.2 exon 75572576 75572616 75572576 ID=MS.gene37570.t1.exon3;Parent=MS.gene37570.t1
chr5.2 CDS 75572576 75572616 75572576 ID=cds.MS.gene37570.t1;Parent=MS.gene37570.t1
chr5.2 exon 75571663 75571722 75571663 ID=MS.gene37570.t1.exon4;Parent=MS.gene37570.t1
chr5.2 CDS 75571663 75571722 75571663 ID=cds.MS.gene37570.t1;Parent=MS.gene37570.t1
chr5.2 exon 75571259 75571312 75571259 ID=MS.gene37570.t1.exon5;Parent=MS.gene37570.t1
chr5.2 CDS 75571259 75571312 75571259 ID=cds.MS.gene37570.t1;Parent=MS.gene37570.t1
Gene Sequence

>MS.gene37570

GAAGAATCATCATCATCATCATCCAGACAATTAACACCTGGATCTGGATCTGGATCTGCACAGGAAGAGGTAGTTGTGGTGAAATTGCTGTGTGTATTGATGTCTGTGTAAACATATAATGCTGTCTAACAAACTTATCACTGCAGCTAAATGAATGGCATGATGCATGGAATGAAAAGGATAAGGATTTTGAGAATCAAGGAATCAACAAACATGGTATCACATATAACCTTATTGTTCAACAAATACATCAAAATTCACGAGTTTCTGAGCAATATTGTTAATTTTGTTACAACAATCAACTGGACTGTGTTTTGTAAATTAATGATTTGCTTATGTGCATTCCCCAAACTAACAGATTCATTTTGGAACAAGCTAAAGTCAGAGGCTGCTAAAAAGGTTTTTTAAATTCTCCTGCTTATTTTTACATGCATACTGATTTTTGTGCTACCATTATCCTTGTTCTTAATTATTAAAAATGGAACATAGCACATGTTTATATTGGAAATTTTCTTGTGCCCTTGTTTACTTTTTCTTTACATTTCAATCTCTATAGCAACATTATAAATTATAAGCTTTAACCTTAAAAGTTTGAGGCTGATTATATGAGCTTTTAACTGAAACTAATTGCAATGCTAGATAGATTCTCTTTATATTCAATTCTAAAGCTTTTTTTTTTCTCAACAACACTTCCAAACCCAGTTTGAATTACTTTGATTTTCATTTATGCATTAGTTAAACTTAAATATTATATTCTTAATTGATTAAACACAAACTCTGTCTTTCTATATAAAAATTCTTGTCCCATCTGGCATGTGAAAAAAATGTAGACTCTTGCATCCTTGCCTCCTTAGTCCTTTCCTTTGAAACCAGTGGCCAGTAGACTTGGTTTGCATGCATAATTGAAAGTTTCATTTAATGATGGACATTTATGTTGATGATTTTGTAAAGATAGGGGAGATTGTTGTTGTAGGAGAAAAGCATTTTTGTCTATCACTTATATGCATGAACATTCTTTAGTTGTCTTCTTTTTCTCTTTATCATGTTTGTGAAGGTATAATTAAACCTAAATTGTCACACGCATAAATAGGGATAAAAGATAAGTGAGAGGGGTACATGTGTGTTGGGCTAGGGTGTTATAACTGTGATGGTGATGTTGCAGAGGATTTATGTCATTTATATGTCATTGTTAAATTAGCTATCAAGAAGACTTAGAATGACACTACGCTCATTGGTTATAATGTTTCAACAGGTTGGTACAGAAGAAGCGGAAAGATTTTGCAAGGCGTTTCAACAAATTCATAAGAGACTAGTACGTTTAAATGATTATCATTTTGGGCATTCTTTCATAGAAAGTATTATGGAAGACTTTTTATTTATTTATAGAAAGTAGTTTCTTGGAAAGCATATTTATTTTCATCGAAAATGAACATTTTGTTTTTTAGGGGTCTTCTTGAAAGAAGTCTTTCATTGATTAGGAAAATAATTTTTTGAACTGCATATTTGCAACAAATATAGAAAATAATATATTTATTTTCTCCATGAAATTCCAGAAAATATAAATGAATAAAATTGATGGCTCATTAAATCAATTGAAACTGAGTTTATGTTGCTATTATATCATGTTTAACCAAAAATTCTTTTATTGTTTTATTTCTTTAGGTCAATGAAGAGTTAAGTTTAGATGTTGCTCGAAAATTGGTAAACTCATCCTAA

Protein sequence

>MS.gene37570.t1

EESSSSSSRQLTPGSGSGSAQEELNEWHDAWNEKDKDFENQGINKHDSFWNKLKSEAAKKVGTEEAERFCKAFQQIHKRLVNEELSLDVARKLVNSS