AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52198


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52198.t1 MTR_7g076620 93.711 159 10 0 1 159 1 159 2.66e-108 305
MS.gene52198.t1 MTR_8g032260 48.592 142 66 3 1 135 1 142 1.10e-39 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52198.t1 AT4G23890 47.368 133 63 3 6 134 7 136 2.79e-32 115

Find 26 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTTCCTGGTGGTGAAAA+AGG 0.216558 7.4:-29049794 MS.gene52198:CDS
TTTATTTCTTCAATAGTAAA+AGG 0.269787 7.4:+29049725 None:intergenic
GGACTATGCAATGGAGAAAA+AGG 0.281632 7.4:-29049974 MS.gene52198:CDS
GGGCTAACTGGAGGGTTTCC+TGG 0.314218 7.4:-29049806 MS.gene52198:CDS
GCTACTCTTCAAGGAATTCA+TGG 0.329958 7.4:-29050136 MS.gene52198:CDS
TCCTTCTCAAAACCATCTTT+AGG 0.343967 7.4:+29049836 None:intergenic
AAGGAAATGATGGGGCTAAC+TGG 0.367060 7.4:-29049818 MS.gene52198:CDS
AGAGTTGAACTATGTTGCTT+TGG 0.385186 7.4:+29049682 None:intergenic
GGTGATCTTGGCCTATGAAC+TGG 0.404590 7.4:+29050098 None:intergenic
CAAGTTGATAAAGATGAAAC+AGG 0.427205 7.4:-29049929 MS.gene52198:CDS
ATTGCTGCAATTCCTAAAGA+TGG 0.439610 7.4:-29049848 MS.gene52198:CDS
CGTGGTTGTAGTTGAGAAGT+TGG 0.442826 7.4:+29050034 None:intergenic
GACTTGAACAAGAGCTGAAA+AGG 0.446004 7.4:-29049952 MS.gene52198:CDS
CTCCTACGATCCCAGTTCAT+AGG 0.487970 7.4:-29050109 MS.gene52198:CDS
TGAGAGTGAAGTGGTGATCT+TGG 0.497067 7.4:+29050086 None:intergenic
AAAGGTTGAGAAGAATTCCT+AGG 0.555335 7.4:+29049743 None:intergenic
GTGATCTTGGCCTATGAACT+GGG 0.572739 7.4:+29050099 None:intergenic
AAAGTGCAAGAAAACTCAGA+TGG 0.584856 7.4:-29049893 MS.gene52198:CDS
AAATGATGGGGCTAACTGGA+GGG 0.586145 7.4:-29049814 MS.gene52198:CDS
GGCCTATGAACTGGGATCGT+AGG 0.587300 7.4:+29050107 None:intergenic
GAAATGATGGGGCTAACTGG+AGG 0.588055 7.4:-29049815 MS.gene52198:CDS
GGAGGAAGAGGACTATGCAA+TGG 0.605614 7.4:-29049983 MS.gene52198:CDS
AAGATTTCATGAGAGTGAAG+TGG 0.619848 7.4:+29050077 None:intergenic
CTAACTGGAGGGTTTCCTGG+TGG 0.624925 7.4:-29049803 MS.gene52198:CDS
GGTTGAGAAGAATTCCTAGG+TGG 0.675895 7.4:+29049746 None:intergenic
AGTATGTTGAATTCAGCACG+TGG 0.711591 7.4:+29050016 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTCTTCAATAGTAAA+AGG + chr7.4:29050117-29050136 None:intergenic 15.0%
!! ATTCAACATACTAAAAATGA+TGG - chr7.4:29049834-29049853 MS.gene52198:CDS 20.0%
!! TTCAACATACTAAAAATGAT+GGG - chr7.4:29049835-29049854 MS.gene52198:CDS 20.0%
! AACATACTAAAAATGATGGG+AGG - chr7.4:29049838-29049857 MS.gene52198:CDS 30.0%
!! CAAGTTGATAAAGATGAAAC+AGG - chr7.4:29049910-29049929 MS.gene52198:CDS 30.0%
!!! GTTTTCTTGCACTTTTTCAT+TGG + chr7.4:29049941-29049960 None:intergenic 30.0%
AAAGGTTGAGAAGAATTCCT+AGG + chr7.4:29050099-29050118 None:intergenic 35.0%
AAAGTGCAAGAAAACTCAGA+TGG - chr7.4:29049946-29049965 MS.gene52198:CDS 35.0%
AAGATTTCATGAGAGTGAAG+TGG + chr7.4:29049765-29049784 None:intergenic 35.0%
ATTGCTGCAATTCCTAAAGA+TGG - chr7.4:29049991-29050010 MS.gene52198:CDS 35.0%
TCATTGAGAAAAATCCACCT+AGG - chr7.4:29050079-29050098 MS.gene52198:CDS 35.0%
TCCTTCTCAAAACCATCTTT+AGG + chr7.4:29050006-29050025 None:intergenic 35.0%
!!! GGTTTTGAGAAGGAAATGAT+GGG - chr7.4:29050012-29050031 MS.gene52198:CDS 35.0%
!!! GTTTTGAGAAGGAAATGATG+GGG - chr7.4:29050013-29050032 MS.gene52198:CDS 35.0%
!!! TCCTAAAGATGGTTTTGAGA+AGG - chr7.4:29050002-29050021 MS.gene52198:CDS 35.0%
!!! TGGTTTTGAGAAGGAAATGA+TGG - chr7.4:29050011-29050030 MS.gene52198:CDS 35.0%
AGTATGTTGAATTCAGCACG+TGG + chr7.4:29049826-29049845 None:intergenic 40.0%
GACTTGAACAAGAGCTGAAA+AGG - chr7.4:29049887-29049906 MS.gene52198:CDS 40.0%
GCTACTCTTCAAGGAATTCA+TGG - chr7.4:29049703-29049722 MS.gene52198:CDS 40.0%
GGACTATGCAATGGAGAAAA+AGG - chr7.4:29049865-29049884 MS.gene52198:CDS 40.0%
! CTAAAAATGATGGGAGGAAG+AGG - chr7.4:29049844-29049863 MS.gene52198:CDS 40.0%
CGTGGTTGTAGTTGAGAAGT+TGG + chr7.4:29049808-29049827 None:intergenic 45.0%
GGTTGAGAAGAATTCCTAGG+TGG + chr7.4:29050096-29050115 None:intergenic 45.0%
GTGATCTTGGCCTATGAACT+GGG + chr7.4:29049743-29049762 None:intergenic 45.0%
TGAGAGTGAAGTGGTGATCT+TGG + chr7.4:29049756-29049775 None:intergenic 45.0%
! AAATGATGGGGCTAACTGGA+GGG - chr7.4:29050025-29050044 MS.gene52198:CDS 45.0%
! AAGGAAATGATGGGGCTAAC+TGG - chr7.4:29050021-29050040 MS.gene52198:CDS 45.0%
! GGTTTCCTGGTGGTGAAAAA+GGG - chr7.4:29050046-29050065 MS.gene52198:CDS 45.0%
!! ATCAACCCTTTTTCACCACC+AGG + chr7.4:29050054-29050073 None:intergenic 45.0%
!!! GCTGCTTTTGCTACTCTTCA+AGG - chr7.4:29049694-29049713 MS.gene52198:CDS 45.0%
CTCCTACGATCCCAGTTCAT+AGG - chr7.4:29049730-29049749 MS.gene52198:CDS 50.0%
GGAGGAAGAGGACTATGCAA+TGG - chr7.4:29049856-29049875 MS.gene52198:CDS 50.0%
GGTGATCTTGGCCTATGAAC+TGG + chr7.4:29049744-29049763 None:intergenic 50.0%
! GAAATGATGGGGCTAACTGG+AGG - chr7.4:29050024-29050043 MS.gene52198:CDS 50.0%
! GGGTTTCCTGGTGGTGAAAA+AGG - chr7.4:29050045-29050064 MS.gene52198:CDS 50.0%
CTAACTGGAGGGTTTCCTGG+TGG - chr7.4:29050036-29050055 MS.gene52198:CDS 55.0%
GGCCTATGAACTGGGATCGT+AGG + chr7.4:29049735-29049754 None:intergenic 55.0%
GGGCTAACTGGAGGGTTTCC+TGG - chr7.4:29050033-29050052 MS.gene52198:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 29049691 29050170 29049691 ID=MS.gene52198
chr7.4 mRNA 29049691 29050170 29049691 ID=MS.gene52198.t1;Parent=MS.gene52198
chr7.4 exon 29049691 29050170 29049691 ID=MS.gene52198.t1.exon1;Parent=MS.gene52198.t1
chr7.4 CDS 29049691 29050170 29049691 ID=cds.MS.gene52198.t1;Parent=MS.gene52198.t1
Gene Sequence

>MS.gene52198

ATGGCTGCTTTTGCTACTCTTCAAGGAATTCATGGCTCTCTCCTACGATCCCAGTTCATAGGCCAAGATCACCACTTCACTCTCATGAAATCTTCAACAACACATCACAGAAAACCAACTTCTCAACTACAACCACGTGCTGAATTCAACATACTAAAAATGATGGGAGGAAGAGGACTATGCAATGGAGAAAAAGGACTTGAACAAGAGCTGAAAAGGCAAGTTGATAAAGATGAAACAGGATCATCCAATGAAAAAGTGCAAGAAAACTCAGATGGATCAGAAAAGTTGACTAGTGATATTGCTGCAATTCCTAAAGATGGTTTTGAGAAGGAAATGATGGGGCTAACTGGAGGGTTTCCTGGTGGTGAAAAAGGGTTGATTAAGTTCATTGAGAAAAATCCACCTAGGAATTCTTCTCAACCTTTTACTATTGAAGAAATAAATCAAAGTCTTGTTGAAGAATCCAAAGCAACATAG

Protein sequence

>MS.gene52198.t1

MAAFATLQGIHGSLLRSQFIGQDHHFTLMKSSTTHHRKPTSQLQPRAEFNILKMMGGRGLCNGEKGLEQELKRQVDKDETGSSNEKVQENSDGSEKLTSDIAAIPKDGFEKEMMGLTGGFPGGEKGLIKFIEKNPPRNSSQPFTIEEINQSLVEESKAT