AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54029


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54029.t1 MTR_2g095130 84.545 220 31 1 1 217 1 220 1.43e-131 369
MS.gene54029.t1 MTR_2g095080 77.928 222 44 2 1 217 1 222 2.82e-117 333
MS.gene54029.t1 MTR_2g095060 76.712 219 49 1 1 217 1 219 3.59e-115 328
MS.gene54029.t1 MTR_2g095170 96.026 151 6 0 1 151 1 151 1.70e-102 294
MS.gene54029.t1 MTR_4g036290 57.639 144 57 3 75 215 44 186 1.58e-50 163
MS.gene54029.t1 MTR_7g077110 58.108 148 57 4 71 215 41 186 1.91e-50 162
MS.gene54029.t1 MTR_7g077050 58.108 148 57 4 71 215 39 184 2.04e-50 162
MS.gene54029.t1 MTR_7g077000 60.156 128 49 2 89 215 2 128 1.60e-48 155
MS.gene54029.t1 MTR_8g031170 60.305 131 50 2 86 215 61 190 3.31e-48 157
MS.gene54029.t1 MTR_7g076990 56.757 148 59 4 71 215 41 186 5.10e-48 156
MS.gene54029.t1 MTR_4g036300 56.757 148 59 4 71 215 41 186 5.10e-48 156
MS.gene54029.t1 MTR_7g012350 56.081 148 60 4 71 215 41 186 1.11e-47 155
MS.gene54029.t1 MTR_7g076920 56.081 148 60 4 71 215 41 186 1.28e-47 155
MS.gene54029.t1 MTR_7g076770 60.938 128 48 2 89 215 61 187 1.79e-47 155
MS.gene54029.t1 MTR_7g077090 53.247 154 64 4 65 215 39 187 7.99e-47 153
MS.gene54029.t1 MTR_7g077010 52.597 154 65 4 65 215 39 187 1.00e-46 153
MS.gene54029.t1 MTR_7g012340 54.730 148 62 4 71 215 41 186 1.10e-46 153
MS.gene54029.t1 MTR_7g076960 55.102 147 62 4 71 215 39 183 2.21e-46 152
MS.gene54029.t1 MTR_7g076970 57.252 131 54 2 86 215 58 187 2.20e-45 150
MS.gene54029.t1 MTR_4g036410 57.252 131 54 2 86 215 39 168 2.75e-45 149
MS.gene54029.t1 MTR_8g031120 51.754 114 43 3 103 215 34 136 3.61e-30 109
MS.gene54029.t1 MTR_8g031150 51.754 114 43 3 103 215 42 144 2.66e-29 107
MS.gene54029.t1 MTR_8g031160 49.107 112 42 2 103 214 24 120 1.22e-26 99.8
MS.gene54029.t1 MTR_7g077040 43.966 116 38 2 86 201 36 124 1.55e-22 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGTTTATGATGTTAAAA+AGG 0.240267 2.3:+6856141 None:intergenic
GTAGCTGTGTGGAATGCTTT+AGG 0.246178 2.3:-6855600 MS.gene54029:CDS
TAGCTGTGTGGAATGCTTTA+GGG 0.269715 2.3:-6855599 MS.gene54029:CDS
TGTCGTAACAATATTGTTGA+TGG 0.347026 2.3:-6855627 MS.gene54029:CDS
TGATTCCCCGTCGCACTCTA+AGG 0.365654 2.3:+6855941 None:intergenic
GAAATAGCATTTGTAAGAAA+TGG 0.387424 2.3:+6856119 None:intergenic
CGTTGTACCATCCGGTGGAC+AGG 0.400359 2.3:+6855775 None:intergenic
CATATGTTGGTCCCAACGTC+GGG 0.409197 2.3:+6856017 None:intergenic
CCAGTTACATGGTCTATGTC+TGG 0.410397 2.3:-6855552 MS.gene54029:CDS
GGCGACAAGTGTAAATCTTC+CGG 0.412697 2.3:-6855966 MS.gene54029:CDS
TTAGAATCACCGCGAGGAAC+GGG 0.413213 2.3:-6855728 MS.gene54029:CDS
TTGTCGCCACTTGGTGGTGG+AGG 0.433015 2.3:+6855981 None:intergenic
CCACTTGGTGGTGGAGGGGA+CGG 0.436308 2.3:+6855987 None:intergenic
CCAGACATAGACCATGTAAC+TGG 0.445756 2.3:+6855552 None:intergenic
GCAACGATGACCCCGACGTT+GGG 0.448979 2.3:-6856028 MS.gene54029:CDS
TGGTGGTGGAGGGGACGGTT+TGG 0.450078 2.3:+6855992 None:intergenic
CTACTCTTTCTGAGTTGCTA+TGG 0.454282 2.3:+6855802 None:intergenic
GTGGAGAGCACTTGAATTGA+GGG 0.454649 2.3:+6855916 None:intergenic
CCGGCACCTTAGAGTGCGAC+GGG 0.479301 2.3:-6855947 MS.gene54029:CDS
AGAGTAGTGGCCCTGTCCAC+CGG 0.488313 2.3:-6855786 MS.gene54029:CDS
CACTTGTCGCCACTTGGTGG+TGG 0.492715 2.3:+6855978 None:intergenic
GATTTCAGCGAGGGTGGCGA+TGG 0.507805 2.3:-6855855 MS.gene54029:CDS
GGTGGAGAGCACTTGAATTG+AGG 0.508261 2.3:+6855915 None:intergenic
TGTCGCCACTTGGTGGTGGA+GGG 0.508955 2.3:+6855982 None:intergenic
ATTAGAATCACCGCGAGGAA+CGG 0.523253 2.3:-6855729 MS.gene54029:CDS
GGACGGTTTGGTGCATATGT+TGG 0.523330 2.3:+6856004 None:intergenic
GTCGCCACTTGGTGGTGGAG+GGG 0.524109 2.3:+6855983 None:intergenic
GACCAGTGTGATTCAGTGAA+TGG 0.526043 2.3:-6855681 MS.gene54029:CDS
GATTTACACTTGTCGCCACT+TGG 0.526065 2.3:+6855972 None:intergenic
TTCAGCGAGGGTGGCGATGG+AGG 0.531365 2.3:-6855852 MS.gene54029:CDS
AATTGCTCGTCGCACTGAGA+TGG 0.531537 2.3:+6855825 None:intergenic
TTACACTTGTCGCCACTTGG+TGG 0.536650 2.3:+6855975 None:intergenic
TGAACCTCCGTTGTACCATC+CGG 0.538055 2.3:+6855767 None:intergenic
GGTCAACTCATTTGCATCAA+CGG 0.540764 2.3:-6856056 MS.gene54029:CDS
TTAGGGTTGGATACTGATGA+TGG 0.540804 2.3:-6855582 MS.gene54029:CDS
CCCGTCGCACTCTAAGGTGC+CGG 0.540944 2.3:+6855947 None:intergenic
GCTTGTGTGGAAGATGAAAC+TGG 0.550048 2.3:+6855894 None:intergenic
GAGGAGTGTGATGGCGAAAG+TGG 0.551844 2.3:-6855706 MS.gene54029:CDS
GCATATGTTGGTCCCAACGT+CGG 0.558869 2.3:+6856016 None:intergenic
GTTGTACCATCCGGTGGACA+GGG 0.562020 2.3:+6855776 None:intergenic
TGCAACGATGACCCCGACGT+TGG 0.562513 2.3:-6856029 MS.gene54029:CDS
AATGCTATTTCCTCATGCAA+TGG 0.571036 2.3:-6856107 MS.gene54029:CDS
TTGAATGATTTCAGCGAGGG+TGG 0.573268 2.3:-6855861 MS.gene54029:CDS
ATGGTGTGGTGCCAGTTACA+TGG 0.574649 2.3:-6855563 MS.gene54029:CDS
GAGGAACGGGAGGAGTGTGA+TGG 0.583244 2.3:-6855715 MS.gene54029:CDS
GAATCACCGCGAGGAACGGG+AGG 0.586047 2.3:-6855725 MS.gene54029:CDS
AAAACTCTCAACGACTGCGC+TGG 0.587040 2.3:-6856077 MS.gene54029:CDS
TTTACAAGGTCCATTGCATG+AGG 0.591735 2.3:+6856097 None:intergenic
ACGTTGAATGATTTCAGCGA+GGG 0.598557 2.3:-6855864 MS.gene54029:CDS
TCCGGCACCTTAGAGTGCGA+CGG 0.625179 2.3:-6855948 MS.gene54029:CDS
CTGTCCACCGGATGGTACAA+CGG 0.625342 2.3:-6855774 MS.gene54029:CDS
GAGTGTGATGGCGAAAGTGG+TGG 0.626341 2.3:-6855703 MS.gene54029:CDS
GTTGGATACTGATGATGGTG+TGG 0.638034 2.3:-6855577 MS.gene54029:CDS
TACAACGGAGGTTCAAGATG+TGG 0.638491 2.3:-6855759 MS.gene54029:CDS
CACACTCCTCCCGTTCCTCG+CGG 0.641101 2.3:+6855719 None:intergenic
TAGTGGCCCTGTCCACCGGA+TGG 0.641578 2.3:-6855782 MS.gene54029:CDS
GGATGTGATGAGGAACATGC+AGG 0.645551 2.3:-6855660 MS.gene54029:CDS
TGTGTGGAATGCTTTAGGGT+TGG 0.646152 2.3:-6855595 MS.gene54029:CDS
CCGTCCCCTCCACCACCAAG+TGG 0.646637 2.3:-6855987 MS.gene54029:CDS
ATCCATTCACTGAATCACAC+TGG 0.661311 2.3:+6855679 None:intergenic
CAACGTGAGTAATGCTTGTG+TGG 0.662007 2.3:+6855881 None:intergenic
ACCTCCGTTGTACCATCCGG+TGG 0.666402 2.3:+6855770 None:intergenic
TAGCAACTCAGAAAGAGTAG+TGG 0.673721 2.3:-6855799 MS.gene54029:CDS
CACGTTGAATGATTTCAGCG+AGG 0.674472 2.3:-6855865 MS.gene54029:CDS
TTCAGTGAATGGATGTGATG+AGG 0.675998 2.3:-6855670 MS.gene54029:CDS
ATATGTTGGTCCCAACGTCG+GGG 0.692316 2.3:+6856018 None:intergenic
ACAATATTGTTACGACAAGG+TGG 0.698524 2.3:+6855633 None:intergenic
TCAACAATATTGTTACGACA+AGG 0.725534 2.3:+6855630 None:intergenic
TTGATGGATCTGTAGCTGTG+TGG 0.729799 2.3:-6855611 MS.gene54029:CDS
CGGCACCTTAGAGTGCGACG+GGG 0.757349 2.3:-6855946 MS.gene54029:CDS
TCCACCGGATGGTACAACGG+AGG 0.763995 2.3:-6855771 MS.gene54029:CDS
TGTGTGGAAGATGAAACTGG+TGG 0.770461 2.3:+6855897 None:intergenic
AAATGATTAGAATCACCGCG+AGG 0.806861 2.3:-6855734 MS.gene54029:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATGTTTATGATGTTAAAA+AGG + chr2.3:6855562-6855581 None:intergenic 20.0%
!! TATGATGTTAAAAAGGAAAA+TGG + chr2.3:6855555-6855574 None:intergenic 20.0%
! GAAATAGCATTTGTAAGAAA+TGG + chr2.3:6855584-6855603 None:intergenic 25.0%
TCAACAATATTGTTACGACA+AGG + chr2.3:6856073-6856092 None:intergenic 30.0%
TGTCGTAACAATATTGTTGA+TGG - chr2.3:6856073-6856092 MS.gene54029:CDS 30.0%
ACAATATTGTTACGACAAGG+TGG + chr2.3:6856070-6856089 None:intergenic 35.0%
!! AATGCTATTTCCTCATGCAA+TGG - chr2.3:6855593-6855612 MS.gene54029:CDS 35.0%
AAATGATTAGAATCACCGCG+AGG - chr2.3:6855966-6855985 MS.gene54029:CDS 40.0%
ACGTTGAATGATTTCAGCGA+GGG - chr2.3:6855836-6855855 MS.gene54029:CDS 40.0%
ATCCATTCACTGAATCACAC+TGG + chr2.3:6856024-6856043 None:intergenic 40.0%
CTACTCTTTCTGAGTTGCTA+TGG + chr2.3:6855901-6855920 None:intergenic 40.0%
GGTCAACTCATTTGCATCAA+CGG - chr2.3:6855644-6855663 MS.gene54029:CDS 40.0%
TAGCAACTCAGAAAGAGTAG+TGG - chr2.3:6855901-6855920 MS.gene54029:CDS 40.0%
TAGCTGTGTGGAATGCTTTA+GGG - chr2.3:6856101-6856120 MS.gene54029:CDS 40.0%
TTAGGGTTGGATACTGATGA+TGG - chr2.3:6856118-6856137 MS.gene54029:CDS 40.0%
TTCAGTGAATGGATGTGATG+AGG - chr2.3:6856030-6856049 MS.gene54029:CDS 40.0%
TTTACAAGGTCCATTGCATG+AGG + chr2.3:6855606-6855625 None:intergenic 40.0%
! CAGTCGTTGAGAGTTTTACA+AGG + chr2.3:6855620-6855639 None:intergenic 40.0%
ATTAGAATCACCGCGAGGAA+CGG - chr2.3:6855971-6855990 MS.gene54029:CDS 45.0%
CAACGTGAGTAATGCTTGTG+TGG + chr2.3:6855822-6855841 None:intergenic 45.0%
CACGTTGAATGATTTCAGCG+AGG - chr2.3:6855835-6855854 MS.gene54029:CDS 45.0%
CCAGACATAGACCATGTAAC+TGG + chr2.3:6856151-6856170 None:intergenic 45.0%
CCAGTTACATGGTCTATGTC+TGG - chr2.3:6856148-6856167 MS.gene54029:CDS 45.0%
GACCAGTGTGATTCAGTGAA+TGG - chr2.3:6856019-6856038 MS.gene54029:CDS 45.0%
GATTTACACTTGTCGCCACT+TGG + chr2.3:6855731-6855750 None:intergenic 45.0%
GCTTGTGTGGAAGATGAAAC+TGG + chr2.3:6855809-6855828 None:intergenic 45.0%
GGCGACAAGTGTAAATCTTC+CGG - chr2.3:6855734-6855753 MS.gene54029:CDS 45.0%
GTAGCTGTGTGGAATGCTTT+AGG - chr2.3:6856100-6856119 MS.gene54029:CDS 45.0%
TACAACGGAGGTTCAAGATG+TGG - chr2.3:6855941-6855960 MS.gene54029:CDS 45.0%
TGTGTGGAAGATGAAACTGG+TGG + chr2.3:6855806-6855825 None:intergenic 45.0%
TTGAATGATTTCAGCGAGGG+TGG - chr2.3:6855839-6855858 MS.gene54029:CDS 45.0%
! TTGATGGATCTGTAGCTGTG+TGG - chr2.3:6856089-6856108 MS.gene54029:CDS 45.0%
!! GTGGAGAGCACTTGAATTGA+GGG + chr2.3:6855787-6855806 None:intergenic 45.0%
!! GTTGGATACTGATGATGGTG+TGG - chr2.3:6856123-6856142 MS.gene54029:CDS 45.0%
!! TGTGTGGAATGCTTTAGGGT+TGG - chr2.3:6856105-6856124 MS.gene54029:CDS 45.0%
AAAACTCTCAACGACTGCGC+TGG - chr2.3:6855623-6855642 MS.gene54029:CDS 50.0%
AATTGCTCGTCGCACTGAGA+TGG + chr2.3:6855878-6855897 None:intergenic 50.0%
ATATGTTGGTCCCAACGTCG+GGG + chr2.3:6855685-6855704 None:intergenic 50.0%
CATATGTTGGTCCCAACGTC+GGG + chr2.3:6855686-6855705 None:intergenic 50.0%
GCATATGTTGGTCCCAACGT+CGG + chr2.3:6855687-6855706 None:intergenic 50.0%
GGATGTGATGAGGAACATGC+AGG - chr2.3:6856040-6856059 MS.gene54029:CDS 50.0%
TGAACCTCCGTTGTACCATC+CGG + chr2.3:6855936-6855955 None:intergenic 50.0%
TTACACTTGTCGCCACTTGG+TGG + chr2.3:6855728-6855747 None:intergenic 50.0%
TTAGAATCACCGCGAGGAAC+GGG - chr2.3:6855972-6855991 MS.gene54029:CDS 50.0%
! ATGGTGTGGTGCCAGTTACA+TGG - chr2.3:6856137-6856156 MS.gene54029:CDS 50.0%
!! GGACGGTTTGGTGCATATGT+TGG + chr2.3:6855699-6855718 None:intergenic 50.0%
!! GGTGGAGAGCACTTGAATTG+AGG + chr2.3:6855788-6855807 None:intergenic 50.0%
CTGTCCACCGGATGGTACAA+CGG - chr2.3:6855926-6855945 MS.gene54029:CDS 55.0%
GTTGTACCATCCGGTGGACA+GGG + chr2.3:6855927-6855946 None:intergenic 55.0%
TGATTCCCCGTCGCACTCTA+AGG + chr2.3:6855762-6855781 None:intergenic 55.0%
! GAGGAGTGTGATGGCGAAAG+TGG - chr2.3:6855994-6856013 MS.gene54029:CDS 55.0%
! GAGTGTGATGGCGAAAGTGG+TGG - chr2.3:6855997-6856016 MS.gene54029:CDS 55.0%
ACCTCCGTTGTACCATCCGG+TGG + chr2.3:6855933-6855952 None:intergenic 60.0%
AGAGTAGTGGCCCTGTCCAC+CGG - chr2.3:6855914-6855933 MS.gene54029:CDS 60.0%
CGTTGTACCATCCGGTGGAC+AGG + chr2.3:6855928-6855947 None:intergenic 60.0%
GATTTCAGCGAGGGTGGCGA+TGG - chr2.3:6855845-6855864 MS.gene54029:CDS 60.0%
GCAACGATGACCCCGACGTT+GGG - chr2.3:6855672-6855691 MS.gene54029:CDS 60.0%
TCCACCGGATGGTACAACGG+AGG - chr2.3:6855929-6855948 MS.gene54029:CDS 60.0%
TCCGGCACCTTAGAGTGCGA+CGG - chr2.3:6855752-6855771 MS.gene54029:CDS 60.0%
TGCAACGATGACCCCGACGT+TGG - chr2.3:6855671-6855690 MS.gene54029:CDS 60.0%
! CACTTGTCGCCACTTGGTGG+TGG + chr2.3:6855725-6855744 None:intergenic 60.0%
! GAGGAACGGGAGGAGTGTGA+TGG - chr2.3:6855985-6856004 MS.gene54029:CDS 60.0%
!! TGTCGCCACTTGGTGGTGGA+GGG + chr2.3:6855721-6855740 None:intergenic 60.0%
!! TTGTCGCCACTTGGTGGTGG+AGG + chr2.3:6855722-6855741 None:intergenic 60.0%
CACACTCCTCCCGTTCCTCG+CGG + chr2.3:6855984-6856003 None:intergenic 65.0%
CCGGCACCTTAGAGTGCGAC+GGG - chr2.3:6855753-6855772 MS.gene54029:CDS 65.0%
CGGCACCTTAGAGTGCGACG+GGG - chr2.3:6855754-6855773 MS.gene54029:CDS 65.0%
GAATCACCGCGAGGAACGGG+AGG - chr2.3:6855975-6855994 MS.gene54029:CDS 65.0%
TAGTGGCCCTGTCCACCGGA+TGG - chr2.3:6855918-6855937 MS.gene54029:CDS 65.0%
TTCAGCGAGGGTGGCGATGG+AGG - chr2.3:6855848-6855867 MS.gene54029:CDS 65.0%
!! CCACTTGGTGGTGGAGGGGA+CGG + chr2.3:6855716-6855735 None:intergenic 65.0%
!! CCCGTCGCACTCTAAGGTGC+CGG + chr2.3:6855756-6855775 None:intergenic 65.0%
!! GTCGCCACTTGGTGGTGGAG+GGG + chr2.3:6855720-6855739 None:intergenic 65.0%
!! TGGTGGTGGAGGGGACGGTT+TGG + chr2.3:6855711-6855730 None:intergenic 65.0%
CCGTCCCCTCCACCACCAAG+TGG - chr2.3:6855713-6855732 MS.gene54029:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 6855518 6856204 6855518 ID=MS.gene54029
chr2.3 mRNA 6855518 6856204 6855518 ID=MS.gene54029.t1;Parent=MS.gene54029
chr2.3 exon 6855518 6856204 6855518 ID=MS.gene54029.t1.exon1;Parent=MS.gene54029.t1
chr2.3 CDS 6855518 6856204 6855518 ID=cds.MS.gene54029.t1;Parent=MS.gene54029.t1
Gene Sequence

>MS.gene54029

ATGGCTCACGTTTCAAATCTTGTTTTTTTAGCTTCCATTTTCCTTTTTAACATCATAAACATTCCATTTCTTACAAATGCTATTTCCTCATGCAATGGACCTTGTAAAACTCTCAACGACTGCGCTGGTCAACTCATTTGCATCAACGGAAAATGCAACGATGACCCCGACGTTGGGACCAACATATGCACCAAACCGTCCCCTCCACCACCAAGTGGCGACAAGTGTAAATCTTCCGGCACCTTAGAGTGCGACGGGGAATCATTCCCTCAATTCAAGTGCTCTCCACCAGTTTCATCTTCCACACAAGCATTACTCACGTTGAATGATTTCAGCGAGGGTGGCGATGGAGGAGCTCCATCTCAGTGCGACGAGCAATTCCATAGCAACTCAGAAAGAGTAGTGGCCCTGTCCACCGGATGGTACAACGGAGGTTCAAGATGTGGAAAAATGATTAGAATCACCGCGAGGAACGGGAGGAGTGTGATGGCGAAAGTGGTGGACCAGTGTGATTCAGTGAATGGATGTGATGAGGAACATGCAGGTCAGCCACCTTGTCGTAACAATATTGTTGATGGATCTGTAGCTGTGTGGAATGCTTTAGGGTTGGATACTGATGATGGTGTGGTGCCAGTTACATGGTCTATGTCTGGATCTAATGAAGCTTATGAAACATTTAATGCCTAA

Protein sequence

>MS.gene54029.t1

MAHVSNLVFLASIFLFNIINIPFLTNAISSCNGPCKTLNDCAGQLICINGKCNDDPDVGTNICTKPSPPPPSGDKCKSSGTLECDGESFPQFKCSPPVSSSTQALLTLNDFSEGGDGGAPSQCDEQFHSNSERVVALSTGWYNGGSRCGKMIRITARNGRSVMAKVVDQCDSVNGCDEEHAGQPPCRNNIVDGSVAVWNALGLDTDDGVVPVTWSMSGSNEAYETFNA