AlfalfaGEDB | MS.gene62558

M. sativa cultivar XinJiangDaYe / MS.gene62558

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62558.t1	XP_004508161.1	96.7	60	2	0	1	60	1	60	1.40E-22	114.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62558.t1	Q9M7X7	83.3	60	10	0	1	60	1	60	9.6e-20	96.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62558.t1	I3SZB6	96.7	60	2	0	1	60	1	60	1.0e-22	114.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene62558	0.834204	3.43E-56	-1.69E-46
MS.gene049341	MS.gene62558	0.804916	1.72E-49	-1.69E-46
MS.gene049756	MS.gene62558	0.822567	2.21E-53	-1.69E-46
MS.gene049821	MS.gene62558	0.819071	1.41E-52	-1.69E-46
MS.gene050277	MS.gene62558	0.854409	1.25E-61	-1.69E-46
MS.gene050303	MS.gene62558	0.850606	1.52E-60	-1.69E-46
MS.gene050438	MS.gene62558	0.80383	2.89E-49	-1.69E-46
MS.gene050807	MS.gene62558	0.810846	9.41E-51	-1.69E-46
MS.gene050815	MS.gene62558	0.827124	1.86E-54	-1.69E-46
MS.gene051063	MS.gene62558	0.809653	1.70E-50	-1.69E-46
MS.gene051243	MS.gene62558	0.810758	9.83E-51	-1.69E-46
MS.gene051245	MS.gene62558	0.804493	2.10E-49	-1.69E-46
MS.gene051328	MS.gene62558	0.849582	2.95E-60	-1.69E-46
MS.gene051330	MS.gene62558	0.845598	3.68E-59	-1.69E-46
MS.gene05134	MS.gene62558	0.857421	1.65E-62	-1.69E-46
MS.gene051451	MS.gene62558	0.820901	5.37E-53	-1.69E-46
MS.gene051633	MS.gene62558	0.812891	3.37E-51	-1.69E-46
MS.gene051700	MS.gene62558	0.818863	1.57E-52	-1.69E-46
MS.gene051746	MS.gene62558	0.82573	4.00E-54	-1.69E-46
MS.gene051748	MS.gene62558	0.82652	2.59E-54	-1.69E-46
MS.gene05188	MS.gene62558	0.847756	9.46E-60	-1.69E-46
MS.gene051903	MS.gene62558	0.838407	2.93E-57	-1.69E-46
MS.gene051975	MS.gene62558	0.807338	5.31E-50	-1.69E-46
MS.gene052012	MS.gene62558	0.802328	5.91E-49	-1.69E-46
MS.gene052133	MS.gene62558	0.822425	2.38E-53	-1.69E-46
MS.gene052286	MS.gene62558	0.821098	4.84E-53	-1.69E-46
MS.gene052302	MS.gene62558	0.827044	1.94E-54	-1.69E-46
MS.gene052376	MS.gene62558	0.846827	1.70E-59	-1.69E-46
MS.gene052947	MS.gene62558	0.866184	3.43E-65	-1.69E-46
MS.gene053074	MS.gene62558	0.810693	1.02E-50	-1.69E-46
MS.gene053109	MS.gene62558	0.811755	5.97E-51	-1.69E-46
MS.gene053226	MS.gene62558	0.813987	1.94E-51	-1.69E-46
MS.gene05364	MS.gene62558	0.800759	1.24E-48	-1.69E-46
MS.gene054657	MS.gene62558	0.818933	1.51E-52	-1.69E-46
MS.gene054658	MS.gene62558	0.804069	2.58E-49	-1.69E-46
MS.gene05517	MS.gene62558	0.802246	6.15E-49	-1.69E-46
MS.gene055468	MS.gene62558	0.807818	4.20E-50	-1.69E-46
MS.gene055608	MS.gene62558	0.812833	3.47E-51	-1.69E-46
MS.gene055623	MS.gene62558	0.823056	1.70E-53	-1.69E-46
MS.gene055808	MS.gene62558	0.844654	6.61E-59	-1.69E-46
MS.gene056091	MS.gene62558	0.813178	2.92E-51	-1.69E-46
MS.gene056432	MS.gene62558	0.807999	3.84E-50	-1.69E-46
MS.gene056580	MS.gene62558	0.803683	3.10E-49	-1.69E-46
MS.gene056631	MS.gene62558	0.886677	2.78E-72	-1.69E-46
MS.gene056760	MS.gene62558	0.826361	2.83E-54	-1.69E-46
MS.gene056986	MS.gene62558	0.800964	1.13E-48	-1.69E-46
MS.gene057094	MS.gene62558	0.801138	1.04E-48	-1.69E-46
MS.gene057140	MS.gene62558	0.850292	1.87E-60	-1.69E-46
MS.gene057234	MS.gene62558	0.811604	6.44E-51	-1.69E-46
MS.gene057693	MS.gene62558	0.80571	1.17E-49	-1.69E-46
MS.gene057705	MS.gene62558	0.809467	1.86E-50	-1.69E-46
MS.gene058055	MS.gene62558	0.802054	6.73E-49	-1.69E-46
MS.gene058084	MS.gene62558	0.829063	6.35E-55	-1.69E-46
MS.gene058168	MS.gene62558	0.802058	6.72E-49	-1.69E-46
MS.gene058238	MS.gene62558	0.822018	2.96E-53	-1.69E-46
MS.gene058906	MS.gene62558	0.875407	3.16E-68	-1.69E-46
MS.gene058908	MS.gene62558	0.81904	1.43E-52	-1.69E-46
MS.gene058911	MS.gene62558	0.823125	1.64E-53	-1.69E-46
MS.gene059249	MS.gene62558	0.818972	1.48E-52	-1.69E-46
MS.gene059317	MS.gene62558	0.813171	2.93E-51	-1.69E-46
MS.gene05954	MS.gene62558	0.82272	2.04E-53	-1.69E-46
MS.gene059898	MS.gene62558	0.802997	4.30E-49	-1.69E-46
MS.gene06001	MS.gene62558	0.886963	2.16E-72	-1.69E-46
MS.gene060101	MS.gene62558	0.804895	1.73E-49	-1.69E-46
MS.gene06011	MS.gene62558	0.809906	1.50E-50	-1.69E-46
MS.gene060429	MS.gene62558	0.909688	4.32E-82	-1.69E-46
MS.gene060586	MS.gene62558	0.8362	1.08E-56	-1.69E-46
MS.gene060907	MS.gene62558	0.809143	2.19E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene043593	MS.gene62558	PPI
MS.gene064353	MS.gene62558	PPI
MS.gene035825	MS.gene62558	PPI
MS.gene73093	MS.gene62558	PPI
MS.gene043643	MS.gene62558	PPI
MS.gene035826	MS.gene62558	PPI
MS.gene064351	MS.gene62558	PPI
MS.gene035828	MS.gene62558	PPI
MS.gene99872	MS.gene62558	PPI
MS.gene043595	MS.gene62558	PPI
MS.gene043645	MS.gene62558	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62558.t1	MTR_4g121877	96.667	60	2	0	1	60	1	60	5.36e-37	117
MS.gene62558.t1	MTR_4g121870	93.333	60	4	0	1	60	137	196	8.56e-36	119

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62558.t1	AT3G06680	83.333	60	10	1	60	23	82	1.68e-30	102
MS.gene62558.t1	AT3G06700	83.333	60	10	1	60	1	60	2.51e-30	100
MS.gene62558.t1	AT3G06700	83.333	60	10	1	60	1	60	2.51e-30	100
MS.gene62558.t1	AT3G06700	83.333	60	10	1	60	1	60	2.51e-30	100
MS.gene62558.t1	AT3G06680	83.333	60	10	1	60	1	60	4.39e-30	100
MS.gene62558.t1	AT3G06680	83.333	60	10	1	60	1	60	4.39e-30	100

Find 13 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTGTGACGATGCCTCTT+TGG	0.332537	7.3:+69045053	None:intergenic
CTCTGGTTCCTCAAGAACTT+TGG	0.404366	7.3:+69044918	None:intergenic
TCTTGTGATCTTTGTAAGAC+TGG	0.406426	7.3:+69045090	None:intergenic
TATGCTTCCTTGCATACCTC+TGG	0.420261	7.3:+69044901	None:intergenic
ATCGTCACACTTCAACCAAA+GGG	0.488627	7.3:-69045043	MS.gene62558:CDS
ATGATGAATACATACCCCTT+TGG	0.493133	7.3:+69045028	None:intergenic
TCTTACAAAGATCACAAGAA+TGG	0.554926	7.3:-69045086	MS.gene62558:CDS
CATCGTCACACTTCAACCAA+AGG	0.595920	7.3:-69045044	MS.gene62558:CDS
AGATGGATCCAAAGTTCTTG+AGG	0.596761	7.3:-69044926	MS.gene62558:CDS
TGAGGAACCAGAGGTATGCA+AGG	0.624057	7.3:-69044908	MS.gene62558:CDS
CAAAGTTCTTGAGGAACCAG+AGG	0.651910	7.3:-69044917	MS.gene62558:CDS
TTTGTAAGACTGGTTGTGAG+CGG	0.659865	7.3:+69045100	None:intergenic
TCGTCACACTTCAACCAAAG+GGG	0.708509	7.3:-69045042	MS.gene62558:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TCTTACAAAGATCACAAGAA+TGG	-	chr7.3:69044902-69044921	MS.gene62558:CDS	30.0%
	TTGTGATTGATTACTGTAGA+TGG	-	chr7.3:69045045-69045064	MS.gene62558:CDS	30.0%
!	AGGAAGCATAACAAGAAAAA+TGG	-	chr7.3:69045100-69045119	MS.gene62558:CDS	30.0%
	ATGATGAATACATACCCCTT+TGG	+	chr7.3:69044963-69044982	None:intergenic	35.0%
	TCTTGTGATCTTTGTAAGAC+TGG	+	chr7.3:69044901-69044920	None:intergenic	35.0%
	AGATGGATCCAAAGTTCTTG+AGG	-	chr7.3:69045062-69045081	MS.gene62558:CDS	40.0%
	ATCGTCACACTTCAACCAAA+GGG	-	chr7.3:69044945-69044964	MS.gene62558:intron	40.0%
	ATGGCATCAAAAAGCCAAAG+AGG	-	chr7.3:69044921-69044940	MS.gene62558:CDS	40.0%
!	TTTGTAAGACTGGTTGTGAG+CGG	+	chr7.3:69044891-69044910	None:intergenic	40.0%
	CAAAGTTCTTGAGGAACCAG+AGG	-	chr7.3:69045071-69045090	MS.gene62558:CDS	45.0%
	CATCGTCACACTTCAACCAA+AGG	-	chr7.3:69044944-69044963	MS.gene62558:intron	45.0%
	TATGCTTCCTTGCATACCTC+TGG	+	chr7.3:69045090-69045109	None:intergenic	45.0%
	TCGTCACACTTCAACCAAAG+GGG	-	chr7.3:69044946-69044965	MS.gene62558:intron	45.0%
!	CTCTGGTTCCTCAAGAACTT+TGG	+	chr7.3:69045073-69045092	None:intergenic	45.0%
	GAAGTGTGACGATGCCTCTT+TGG	+	chr7.3:69044938-69044957	None:intergenic	50.0%
!	TGAGGAACCAGAGGTATGCA+AGG	-	chr7.3:69045080-69045099	MS.gene62558:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	69044866	69045144	69044866	ID=MS.gene62558
chr7.3	mRNA	69044866	69045144	69044866	ID=MS.gene62558.t1;Parent=MS.gene62558
chr7.3	exon	69045043	69045144	69045043	ID=MS.gene62558.t1.exon1;Parent=MS.gene62558.t1
chr7.3	CDS	69045043	69045144	69045043	ID=cds.MS.gene62558.t1;Parent=MS.gene62558.t1
chr7.3	exon	69044866	69044946	69044866	ID=MS.gene62558.t1.exon2;Parent=MS.gene62558.t1
chr7.3	CDS	69044866	69044946	69044866	ID=cds.MS.gene62558.t1;Parent=MS.gene62558.t1

Gene Sequence	>MS.gene62558 ATGGCGAAATCGAAGAATCACACCGCTCACAACCAGTCTTACAAAGATCACAAGAATGGCATCAAAAAGCCAAAGAGGCATCGTCACACTTCAACCAAAGGGGTATGTATTCATCATTCTCATCTGATTTTAAACGATTGATCTATTCTGTAATGAGATTTTAATTAATTGTTTATTTATTGTGATTGATTACTGTAGATGGATCCAAAGTTCTTGAGGAACCAGAGGTATGCAAGGAAGCATAACAAGAAAAATGGTGAAATTGCAGAAGAAGAGTAA
Protein sequence	>MS.gene62558.t1 MAKSKNHTAHNQSYKDHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIAEEE