AlfalfaGEDB | MS.gene67555

M. sativa cultivar XinJiangDaYe / MS.gene67555

KEGG: K21777
GO: GO:0005575 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0110165
PFAM: PF00134.26 PF02984.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67555.t1	CAA55272.1	99.5	411	2	0	2	412	18	428	4.90E-215	756.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67555.t1	P46277	99.0	411	4	0	2	412	18	428	8.4e-216	750.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67555.t1	Q40337	99.5	411	2	0	2	412	18	428	3.5e-215	756.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049251	MS.gene67555	0.824265	8.86E-54	-1.69E-46
MS.gene049596	MS.gene67555	0.810054	1.39E-50	-1.69E-46
MS.gene049844	MS.gene67555	0.806802	6.89E-50	-1.69E-46
MS.gene049989	MS.gene67555	0.852803	3.63E-61	-1.69E-46
MS.gene050297	MS.gene67555	0.842776	2.10E-58	-1.69E-46
MS.gene050875	MS.gene67555	0.820026	8.53E-53	-1.69E-46
MS.gene05124	MS.gene67555	0.832537	8.93E-56	-1.69E-46
MS.gene051742	MS.gene67555	0.816483	5.40E-52	-1.69E-46
MS.gene051816	MS.gene67555	0.827149	1.83E-54	-1.69E-46
MS.gene051896	MS.gene67555	0.823505	1.34E-53	-1.69E-46
MS.gene051914	MS.gene67555	0.875929	2.09E-68	-1.69E-46
MS.gene051955	MS.gene67555	0.839381	1.64E-57	-1.69E-46
MS.gene052143	MS.gene67555	0.857442	1.63E-62	-1.69E-46
MS.gene052169	MS.gene67555	0.821273	4.41E-53	-1.69E-46
MS.gene052308	MS.gene67555	0.892405	1.64E-74	-1.69E-46
MS.gene052367	MS.gene67555	0.910505	1.74E-82	-1.69E-46
MS.gene053173	MS.gene67555	0.904529	1.12E-79	-1.69E-46
MS.gene053348	MS.gene67555	0.860423	2.09E-63	-1.69E-46
MS.gene053416	MS.gene67555	0.854557	1.14E-61	-1.69E-46
MS.gene053417	MS.gene67555	0.854979	8.58E-62	-1.69E-46
MS.gene05369	MS.gene67555	0.818142	2.29E-52	-1.69E-46
MS.gene054459	MS.gene67555	0.834592	2.74E-56	-1.69E-46
MS.gene054551	MS.gene67555	0.901567	2.35E-78	-1.69E-46
MS.gene054552	MS.gene67555	0.835633	1.50E-56	-1.69E-46
MS.gene054593	MS.gene67555	0.902632	7.95E-79	-1.69E-46
MS.gene054633	MS.gene67555	0.859891	3.02E-63	-1.69E-46
MS.gene054643	MS.gene67555	0.805638	1.21E-49	-1.69E-46
MS.gene054662	MS.gene67555	0.878004	3.99E-69	-1.69E-46
MS.gene054885	MS.gene67555	0.835307	1.81E-56	-1.69E-46
MS.gene054941	MS.gene67555	0.804228	2.39E-49	-1.69E-46
MS.gene055308	MS.gene67555	0.909194	7.46E-82	-1.69E-46
MS.gene055578	MS.gene67555	0.905368	4.62E-80	-1.69E-46
MS.gene055579	MS.gene67555	0.827261	1.73E-54	-1.69E-46
MS.gene055957	MS.gene67555	0.871656	5.79E-67	-1.69E-46
MS.gene056041	MS.gene67555	0.920484	1.21E-87	-1.69E-46
MS.gene056042	MS.gene67555	0.824164	9.36E-54	-1.69E-46
MS.gene05640	MS.gene67555	0.907342	5.62E-81	-1.69E-46
MS.gene056526	MS.gene67555	0.847184	1.36E-59	-1.69E-46
MS.gene056528	MS.gene67555	0.84675	1.78E-59	-1.69E-46
MS.gene05652	MS.gene67555	0.871764	5.33E-67	-1.69E-46
MS.gene05732	MS.gene67555	0.826625	2.45E-54	-1.69E-46
MS.gene057367	MS.gene67555	0.851962	6.30E-61	-1.69E-46
MS.gene057458	MS.gene67555	0.893921	4.01E-75	-1.69E-46
MS.gene057483	MS.gene67555	0.815281	1.00E-51	-1.69E-46
MS.gene057669	MS.gene67555	0.810075	1.38E-50	-1.69E-46
MS.gene058013	MS.gene67555	0.805991	1.02E-49	-1.69E-46
MS.gene058128	MS.gene67555	0.891141	5.21E-74	-1.69E-46
MS.gene058818	MS.gene67555	0.822554	2.23E-53	-1.69E-46
MS.gene058844	MS.gene67555	0.800478	1.41E-48	-1.69E-46
MS.gene058849	MS.gene67555	0.825387	4.82E-54	-1.69E-46
MS.gene058853	MS.gene67555	0.897745	1.04E-76	-1.69E-46
MS.gene05926	MS.gene67555	0.80246	5.55E-49	-1.69E-46
MS.gene059553	MS.gene67555	0.858381	8.57E-63	-1.69E-46
MS.gene059616	MS.gene67555	0.867719	1.11E-65	-1.69E-46
MS.gene060473	MS.gene67555	0.883177	5.60E-71	-1.69E-46
MS.gene060565	MS.gene67555	0.867327	1.49E-65	-1.69E-46
MS.gene06093	MS.gene67555	0.918084	2.41E-86	-1.69E-46
MS.gene060985	MS.gene67555	0.824552	7.59E-54	-1.69E-46
MS.gene061099	MS.gene67555	0.896106	5.07E-76	-1.69E-46
MS.gene061260	MS.gene67555	0.898793	3.74E-77	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene67555	MS.gene017693	PPI
MS.gene67555	MS.gene28739	PPI
MS.gene67555	MS.gene072963	PPI
MS.gene67555	MS.gene067143	PPI
MS.gene67555	MS.gene36424	PPI
MS.gene67555	MS.gene27222	PPI
MS.gene67555	MS.gene028366	PPI
MS.gene67555	MS.gene71482	PPI
MS.gene67555	MS.gene072956	PPI
MS.gene67555	MS.gene013970	PPI
MS.gene017542	MS.gene67555	PPI
MS.gene67555	MS.gene000462	PPI
MS.gene67555	MS.gene015942	PPI
MS.gene67555	MS.gene067144	PPI
MS.gene67555	MS.gene55900	PPI
MS.gene67555	MS.gene072954	PPI
MS.gene67555	MS.gene021632	PPI
MS.gene67555	MS.gene014786	PPI
MS.gene67555	MS.gene58835	PPI
MS.gene67555	MS.gene23792	PPI
MS.gene67884	MS.gene67555	PPI
MS.gene67555	MS.gene072952	PPI
MS.gene35086	MS.gene67555	PPI
MS.gene67555	MS.gene017692	PPI
MS.gene67555	MS.gene00063	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67555.t1	MTR_5g023790	98.537	410	6	0	2	411	18	427	0.0	844
MS.gene67555.t1	MTR_5g023790	98.626	364	5	0	2	365	18	381	0.0	749
MS.gene67555.t1	MTR_5g023790	90.294	340	20	2	2	328	18	357	0.0	626
MS.gene67555.t1	MTR_8g074000	74.564	401	98	2	11	411	35	431	0.0	615
MS.gene67555.t1	MTR_5g088980	46.494	271	142	3	139	407	148	417	1.04e-78	249
MS.gene67555.t1	MTR_7g089080	46.763	278	137	3	139	408	182	456	3.23e-77	260
MS.gene67555.t1	MTR_1g011470	34.521	365	208	8	69	403	52	415	4.73e-60	201
MS.gene67555.t1	MTR_3g107970	41.961	255	141	3	156	403	213	467	5.12e-57	194
MS.gene67555.t1	MTR_3g088415	37.847	288	161	8	129	404	216	497	2.12e-56	193
MS.gene67555.t1	MTR_2g102520	41.975	243	132	5	130	364	189	430	7.61e-53	183
MS.gene67555.t1	MTR_3g088405	36.429	280	167	6	140	409	228	506	1.42e-51	181
MS.gene67555.t1	MTR_2g102550	37.413	286	167	5	121	395	129	413	8.13e-51	177
MS.gene67555.t1	MTR_3g088415	41.228	228	124	5	129	349	216	440	2.23e-50	176
MS.gene67555.t1	MTR_1g018680	35.769	260	158	4	149	399	84	343	5.08e-46	162
MS.gene67555.t1	MTR_3g102510	34.263	251	158	3	156	399	74	324	1.57e-43	155
MS.gene67555.t1	MTR_3g060770	39.648	227	126	5	154	373	30	252	4.76e-42	150
MS.gene67555.t1	MTR_3g102520	33.984	256	160	4	153	399	88	343	4.30e-41	149
MS.gene67555.t1	MTR_3g102560	34.728	239	142	4	154	383	62	295	8.60e-40	145
MS.gene67555.t1	MTR_3g102530	33.333	276	168	5	140	399	61	336	1.62e-39	145
MS.gene67555.t1	MTR_3g088415	43.114	167	93	2	137	302	222	387	3.28e-39	145
MS.gene67555.t1	MTR_3g061680	36.726	226	132	5	154	372	20	241	5.31e-37	136
MS.gene67555.t1	MTR_3g060820	36.283	226	133	5	154	372	20	241	6.91e-36	133
MS.gene67555.t1	MTR_3g060400	35.193	233	140	5	147	372	19	247	4.30e-35	131
MS.gene67555.t1	MTR_3g460990	35.193	233	140	5	147	372	19	247	4.30e-35	131
MS.gene67555.t1	MTR_3g102540	33.200	250	150	6	160	399	13	255	5.81e-35	130
MS.gene67555.t1	MTR_3g060410	37.688	199	117	3	182	373	129	327	6.37e-34	130
MS.gene67555.t1	MTR_6g045270	32.143	252	152	8	155	399	45	284	1.15e-26	109
MS.gene67555.t1	MTR_5g011900	30.638	235	142	5	109	328	13	241	1.63e-24	101
MS.gene67555.t1	MTR_1g032850	32.569	218	138	4	158	367	336	552	6.94e-18	85.9
MS.gene67555.t1	MTR_5g044040	46.753	77	41	0	210	286	7	83	5.53e-17	77.4
MS.gene67555.t1	MTR_2g005960	25.688	218	124	6	50	249	32	229	1.60e-13	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67555.t1	AT1G20610	59.305	403	156	5	9	408	32	429	6.19e-170	483
MS.gene67555.t1	AT1G76310	59.524	420	148	9	2	408	20	430	2.00e-169	481
MS.gene67555.t1	AT1G76310	55.804	448	148	10	2	408	20	458	1.14e-162	465
MS.gene67555.t1	AT2G17620	59.662	414	141	8	6	408	25	423	1.28e-159	456
MS.gene67555.t1	AT4G35620	59.048	420	138	10	6	410	27	427	5.13e-150	432
MS.gene67555.t1	AT1G76310	58.090	377	136	9	2	365	20	387	4.74e-144	415
MS.gene67555.t1	AT4G35620	59.091	374	120	9	6	365	27	381	8.33e-131	381
MS.gene67555.t1	AT1G76310	56.918	318	115	9	2	306	20	328	5.37e-112	332
MS.gene67555.t1	AT3G11520	38.987	395	214	8	35	410	26	412	2.30e-86	269
MS.gene67555.t1	AT1G20590	70.175	171	51	0	216	386	65	235	4.20e-86	263
MS.gene67555.t1	AT5G06150	36.643	423	224	8	11	408	30	433	3.17e-85	267
MS.gene67555.t1	AT2G26760	46.183	262	138	3	135	394	109	369	1.02e-78	248
MS.gene67555.t1	AT1G16330	44.444	279	153	1	125	403	366	642	3.35e-78	254
MS.gene67555.t1	AT4G37490	44.718	284	150	4	131	408	140	422	5.08e-76	243
MS.gene67555.t1	AT3G11520	39.429	350	185	8	35	365	26	367	1.39e-75	240
MS.gene67555.t1	AT1G77390	39.405	269	155	4	139	400	162	429	1.40e-59	200
MS.gene67555.t1	AT5G25380	33.957	374	229	8	35	399	59	423	1.47e-58	197
MS.gene67555.t1	AT5G25380	33.957	374	229	8	35	399	59	423	1.47e-58	197
MS.gene67555.t1	AT5G25380	34.921	378	226	9	35	399	59	429	5.88e-58	196
MS.gene67555.t1	AT5G25380	33.875	369	226	8	35	394	59	418	1.94e-57	194
MS.gene67555.t1	AT5G11300	42.379	269	147	4	139	400	156	423	9.15e-57	192
MS.gene67555.t1	AT5G43080	37.548	261	160	3	141	399	73	332	1.95e-55	187
MS.gene67555.t1	AT5G06150	36.585	328	162	9	11	313	30	336	6.02e-55	185
MS.gene67555.t1	AT1G44110	37.050	278	167	4	130	400	169	445	1.36e-54	187
MS.gene67555.t1	AT1G47210	39.552	268	152	5	141	399	88	354	6.97e-54	183
MS.gene67555.t1	AT5G43080	36.842	266	160	4	141	399	73	337	3.88e-53	181
MS.gene67555.t1	AT1G15570	39.700	267	153	4	140	399	173	438	4.26e-53	183
MS.gene67555.t1	AT1G80370	39.700	267	153	4	140	399	182	447	4.56e-53	184
MS.gene67555.t1	AT1G47220	39.925	268	151	5	141	399	42	308	4.22e-52	177
MS.gene67555.t1	AT5G11300	45.946	222	112	4	139	353	156	376	4.89e-52	179
MS.gene67555.t1	AT1G47230	36.154	260	157	4	149	399	88	347	5.41e-48	168
MS.gene67555.t1	AT1G47230	36.015	261	157	5	149	399	88	348	2.89e-46	163
MS.gene67555.t1	AT1G34460	31.987	297	155	6	11	303	142	395	1.33e-37	142
MS.gene67555.t1	AT1G34460	31.987	297	155	6	11	303	142	395	1.33e-37	142
MS.gene67555.t1	AT1G34460	33.000	300	151	9	11	303	128	384	1.98e-37	142
MS.gene67555.t1	AT1G34460	33.000	300	151	9	11	303	142	398	2.58e-37	142
MS.gene67555.t1	AT1G34460	33.000	300	151	9	11	303	142	398	2.58e-37	142
MS.gene67555.t1	AT1G34460	33.000	300	151	9	11	303	142	398	2.58e-37	142
MS.gene67555.t1	AT1G34460	33.000	300	151	9	11	303	142	398	2.58e-37	142
MS.gene67555.t1	AT1G34460	33.000	300	151	9	11	303	142	398	3.17e-37	141
MS.gene67555.t1	AT1G34460	33.000	300	151	9	11	303	142	398	3.26e-37	141
MS.gene67555.t1	AT1G14750	33.696	184	119	2	187	367	385	568	7.71e-21	95.1
MS.gene67555.t1	AT1G14750	33.696	184	119	2	187	367	385	568	7.73e-21	95.1
MS.gene67555.t1	AT1G14750	33.696	184	119	2	187	367	217	400	1.19e-20	93.6
MS.gene67555.t1	AT1G47210	44.762	105	54	3	141	242	88	191	1.28e-20	89.4
MS.gene67555.t1	AT1G14750	33.696	184	118	3	187	367	217	399	1.57e-19	90.5

Find 82 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAGAATGCTCTAGTTTAA+TGG	0.170801	5.4:-15285937	MS.gene67555:CDS
CCATTTCCAGAACTTCTTTC+CGG	0.210345	5.4:+15287069	None:intergenic
TTTCATGAGAAGGTTCTTAA+AGG	0.288446	5.4:-15286731	MS.gene67555:CDS
GGGTGTGATTAATCAGAATT+TGG	0.301953	5.4:-15289129	MS.gene67555:CDS
AAATCAAGTTCAAATGGATT+TGG	0.319295	5.4:-15288316	MS.gene67555:CDS
TGGTTTAGTGGCAATGCTTT+TGG	0.341737	5.4:-15287162	MS.gene67555:CDS
CTGTGTTTCACCAAACTATA+TGG	0.349277	5.4:-15287543	MS.gene67555:CDS
CATTTCCAGAACTTCTTTCC+GGG	0.354928	5.4:+15287070	None:intergenic
GCTGGGACAGGGAAACTTAC+AGG	0.367597	5.4:-15285897	MS.gene67555:CDS
GCTTCAGTTGGTTGGTTTAG+TGG	0.367934	5.4:-15287174	MS.gene67555:CDS
TGAAAGGATGAGGGCTATAC+TGG	0.375569	5.4:-15287501	MS.gene67555:CDS
TTCTCCAGAAGAAAACTTGC+TGG	0.385990	5.4:+15285822	None:intergenic
AGTTGAGATGGAGGATATCA+TGG	0.409043	5.4:-15288084	MS.gene67555:CDS
TTGGCAAAATCAAGTTCAAA+TGG	0.410685	5.4:-15288322	MS.gene67555:CDS
TGCTCTAAAGCCATTGGCAC+TGG	0.419967	5.4:+15288247	None:intergenic
GCAAGAGGGTCATTTGCATC+AGG	0.421294	5.4:+15288035	None:intergenic
GGGGTGTCAATGTCCATAAC+AGG	0.441867	5.4:+15288056	None:intergenic
TCTGTTTGCTCTAAAGCCAT+TGG	0.446717	5.4:+15288241	None:intergenic
CTAGTGATGGGTCGATGAAC+CGG	0.449551	5.4:+15288919	None:intergenic
TGAGAAGAAACAAGCGGATC+CGG	0.456269	5.4:-15288938	MS.gene67555:CDS
CTTATAGGAGGAAGTTGAGA+TGG	0.464867	5.4:-15288096	MS.gene67555:intron
AATTGCTGTGCCATATAGTT+TGG	0.468094	5.4:+15287533	None:intergenic
TATCATGGAAGAGCCTGTTA+TGG	0.468278	5.4:-15288069	MS.gene67555:CDS
GGGTTAGATTCTAGAAAGGT+TGG	0.473549	5.4:-15289170	MS.gene67555:CDS
TTGACATTAATGAAAGGATG+AGG	0.477955	5.4:-15287511	MS.gene67555:CDS
GGCACTGGCTGATCCTCCAC+TGG	0.480548	5.4:+15288262	None:intergenic
GAGTTGATCTTCAGAGTAGT+TGG	0.480770	5.4:+15286413	None:intergenic
GTTGACTTTCACAAGAAGGC+TGG	0.486106	5.4:-15285915	MS.gene67555:CDS
TTTCACAAGAAGGCTGGGAC+AGG	0.486235	5.4:-15285909	MS.gene67555:CDS
ACAGCTCAATGCACTATGTA+TGG	0.490108	5.4:-15286471	MS.gene67555:CDS
CCGGAAAGAAGTTCTGGAAA+TGG	0.494858	5.4:-15287069	MS.gene67555:intron
GGGCAAAACAGAAGAGCATT+GGG	0.497902	5.4:-15289149	MS.gene67555:CDS
AGGAGGGTTAGATTCTAGAA+AGG	0.500876	5.4:-15289174	MS.gene67555:CDS
GGTTAGATTCTAGAAAGGTT+GGG	0.502394	5.4:-15289169	MS.gene67555:CDS
ATACACCCGGAAAGAAGTTC+TGG	0.504153	5.4:-15287075	MS.gene67555:CDS
CGATCTGATTCGCTATGCAT+TGG	0.506937	5.4:+15288217	None:intergenic
ACTGGTTGACTGGCTTATTG+AGG	0.508362	5.4:-15287483	MS.gene67555:intron
TGGGCAAAACAGAAGAGCAT+TGG	0.511695	5.4:-15289150	MS.gene67555:CDS
AACTACCAAAAGGTCAAACT+TGG	0.515338	5.4:-15288341	MS.gene67555:CDS
AGAAAGAAGCTTCAGTTGGT+TGG	0.515982	5.4:-15287182	MS.gene67555:CDS
AATCAGAATTTGGTTGTGGA+AGG	0.518311	5.4:-15289119	MS.gene67555:CDS
ATCAATTCCTCACCTAGTGA+TGG	0.523848	5.4:+15288906	None:intergenic
AATGGTTGACTTTCACAAGA+AGG	0.528684	5.4:-15285919	MS.gene67555:CDS
GCAGCGGCAGCTAGTTGAGA+AGG	0.533466	5.4:+15286501	None:intergenic
TTGTTAACAACACAAGGATA+AGG	0.536421	5.4:+15289092	None:intergenic
ATAGGAGGAAGTTGAGATGG+AGG	0.543444	5.4:-15288093	MS.gene67555:intron
CGGTTCATCGACCCATCACT+AGG	0.545956	5.4:-15288918	MS.gene67555:intron
GAACCTTCTCATGAAAACAT+AGG	0.546868	5.4:+15286738	None:intergenic
GATTAATCAGAATTTGGTTG+TGG	0.548543	5.4:-15289123	MS.gene67555:CDS
ATCCTTGTGTTGTTAACAAG+AGG	0.554542	5.4:-15289088	MS.gene67555:CDS
TGAGGGCTATACTGGTTGAC+TGG	0.555400	5.4:-15287493	MS.gene67555:CDS
GGAGGATCAGCCAGTGCCAA+TGG	0.555755	5.4:-15288257	MS.gene67555:CDS
CTCTTACTACAGAAAAGTTG+AGG	0.560855	5.4:-15287985	MS.gene67555:intron
AGCAATTTGACATTAATGAA+AGG	0.565905	5.4:-15287517	MS.gene67555:CDS
TCCTTGTGTTGTTAACAAGA+GGG	0.566377	5.4:-15289087	MS.gene67555:CDS
TTGAATAGGGAACTACCAAA+AGG	0.566910	5.4:-15288351	MS.gene67555:intron
CAAGAGGGTCATTTGCATCA+GGG	0.569803	5.4:+15288036	None:intergenic
TTGACTTTCACAAGAAGGCT+GGG	0.569949	5.4:-15285914	MS.gene67555:CDS
GAAGTTTCAGTGCCTGTGGT+TGG	0.581724	5.4:-15287125	MS.gene67555:CDS
GGTAAGAAAGAAGCTTCAGT+TGG	0.588907	5.4:-15287186	MS.gene67555:CDS
CTCATGAAAACATAGGCTGT+CGG	0.589074	5.4:+15286745	None:intergenic
TCAATTCCTCACCTAGTGAT+GGG	0.589190	5.4:+15288907	None:intergenic
TGACATTAATGAAAGGATGA+GGG	0.591362	5.4:-15287510	MS.gene67555:CDS
CATGAGAAGGTTCTTAAAGG+CGG	0.594323	5.4:-15286728	MS.gene67555:CDS
AAGAGGGTCATTTGCATCAG+GGG	0.596917	5.4:+15288037	None:intergenic
ATATATTCAGCAACTGCAAG+AGG	0.601924	5.4:+15288020	None:intergenic
GCATAGCGAATCAGATCGGA+TGG	0.604904	5.4:-15288212	MS.gene67555:intron
GCCCTCTTGTTAACAACACA+AGG	0.607827	5.4:+15289086	None:intergenic
AGAATTAGATCTCCAACCAC+AGG	0.618555	5.4:+15287113	None:intergenic
CAATGCATAGCGAATCAGAT+CGG	0.621608	5.4:-15288216	MS.gene67555:CDS
TATCAGACAGAGCATACACC+CGG	0.634548	5.4:-15287088	MS.gene67555:CDS
AGTGGAGTAAGACATGTGAA+TGG	0.637958	5.4:-15286440	MS.gene67555:CDS
TTCACAAGAAGGCTGGGACA+GGG	0.640634	5.4:-15285908	MS.gene67555:CDS
TGAGGAAGTTTCAGTGCCTG+TGG	0.654299	5.4:-15287129	MS.gene67555:CDS
TATATTCAGCAACTGCAAGA+GGG	0.656026	5.4:+15288021	None:intergenic
GGAAACTTACAGGAGCACAT+AGG	0.667527	5.4:-15285887	MS.gene67555:CDS
TGATGAACACAAGCCAGTGG+AGG	0.670864	5.4:-15288275	MS.gene67555:CDS
GAAACAATGTCTCATGCATG+AGG	0.677202	5.4:+15287252	None:intergenic
CTATGTATGGTGTCAAACAG+TGG	0.683237	5.4:-15286458	MS.gene67555:CDS
TTGAGCTGTATAGACAGCAG+CGG	0.686693	5.4:+15286485	None:intergenic
TGATGATGAACACAAGCCAG+TGG	0.720589	5.4:-15288278	MS.gene67555:CDS
TGTTTGTGAGAAGAAACAAG+CGG	0.747744	5.4:-15288944	MS.gene67555:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACAACTAAAATTTAAAAAA+AGG	+	chr5.4:15286914-15286933	None:intergenic	10.0%
!!	TATGATAAAGAAAAAAAAAA+TGG	+	chr5.4:15288398-15288417	None:intergenic	10.0%
!!	TATTAACTAATTAAATAAAC+AGG	+	chr5.4:15286875-15286894	None:intergenic	10.0%
!!	AAAACAATAATTGAATGTAA+TGG	-	chr5.4:15287179-15287198	MS.gene67555:CDS	15.0%
!!	ACTATATAAAAAAAAGGTTA+TGG	+	chr5.4:15289026-15289045	None:intergenic	15.0%
!!!	AAAATTCAAGAGTATAAATT+TGG	+	chr5.4:15287377-15287396	None:intergenic	15.0%
!!!	AATATCTTTGTTTTTTCTTA+TGG	+	chr5.4:15286230-15286249	None:intergenic	15.0%
!!!	ATTTGTTATTGAAATTTGAT+TGG	+	chr5.4:15287458-15287477	None:intergenic	15.0%
!!!	TTAATTGTTTTTGTGATAAT+AGG	-	chr5.4:15285853-15285872	MS.gene67555:CDS	15.0%
!!	AGAAATTCAAATTACACTAA+AGG	+	chr5.4:15286196-15286215	None:intergenic	20.0%
!!	ATATAACAAAATGACATCAT+AGG	-	chr5.4:15288955-15288974	MS.gene67555:CDS	20.0%
!!	ATTAATCCTATATTTAATCC+AGG	+	chr5.4:15287646-15287665	None:intergenic	20.0%
!!	GAAATTCAAATTACACTAAA+GGG	+	chr5.4:15286195-15286214	None:intergenic	20.0%
!!	GTATTTGTAATTAGTAACTA+TGG	+	chr5.4:15288431-15288450	None:intergenic	20.0%
!!	TAAAGAAAAAAAAAATGGCA+TGG	+	chr5.4:15288393-15288412	None:intergenic	20.0%
!!	TATAACAAAATGACATCATA+GGG	-	chr5.4:15288956-15288975	MS.gene67555:CDS	20.0%
!!	TCTTGATTTACTGTTATATT+AGG	-	chr5.4:15286543-15286562	MS.gene67555:CDS	20.0%
!!	TTTAGTGTAATTTGAATTTC+TGG	-	chr5.4:15286195-15286214	MS.gene67555:intron	20.0%
!!	TTTCAGAATTCCAATTAATA+TGG	-	chr5.4:15287423-15287442	MS.gene67555:intron	20.0%
!!!	ATTTGTGTGTAAAATTTTGT+AGG	-	chr5.4:15288238-15288257	MS.gene67555:CDS	20.0%
!	AAATGGCATGGAAATATATT+AGG	+	chr5.4:15288381-15288400	None:intergenic	25.0%
!	AAGTAAGCATTATCCATATT+TGG	+	chr5.4:15287736-15287755	None:intergenic	25.0%
!	AATGTAGCAAAATTTCACAA+AGG	+	chr5.4:15286629-15286648	None:intergenic	25.0%
!	AATTCACAATCACAAAAAGA+AGG	+	chr5.4:15286021-15286040	None:intergenic	25.0%
!	AGCAATTTGACATTAATGAA+AGG	-	chr5.4:15287530-15287549	MS.gene67555:CDS	25.0%
!	AGTAAGCATTATCCATATTT+GGG	+	chr5.4:15287735-15287754	None:intergenic	25.0%
!	ATTCACAATCACAAAAAGAA+GGG	+	chr5.4:15286020-15286039	None:intergenic	25.0%
!	CACAAATGCTATAAATGTAA+AGG	+	chr5.4:15288036-15288055	None:intergenic	25.0%
!	CATTCAAAATCTTAGTCAAA+AGG	+	chr5.4:15286665-15286684	None:intergenic	25.0%
!	CATTTGAACTAAAATCCAAA+TGG	+	chr5.4:15287115-15287134	None:intergenic	25.0%
!	CTTTACATTTATAGCATTTG+TGG	-	chr5.4:15288034-15288053	MS.gene67555:CDS	25.0%
!	GAAAGAATTGCCATATTAAT+TGG	+	chr5.4:15287436-15287455	None:intergenic	25.0%
!	TGATTTACTGTTATATTAGG+AGG	-	chr5.4:15286546-15286565	MS.gene67555:CDS	25.0%
!	TTGAGATAAGTGTTTGAATT+TGG	-	chr5.4:15286248-15286267	MS.gene67555:intron	25.0%
!!	AAATCAAGTTCAAATGGATT+TGG	-	chr5.4:15286731-15286750	MS.gene67555:CDS	25.0%
!!	AAGTTTTGCTAAAATGATGA+TGG	-	chr5.4:15286515-15286534	MS.gene67555:CDS	25.0%
!!	CAATCTTATAGACAGATTTT+TGG	-	chr5.4:15287825-15287844	MS.gene67555:intron	25.0%
!!	GAATTGATGATTTAAGTTTC+TGG	-	chr5.4:15286156-15286175	MS.gene67555:intron	25.0%
!!	TGACTAAGATTTTGAATGTT+GGG	-	chr5.4:15286667-15286686	MS.gene67555:intron	25.0%
!!	TGTAATTTGAATTTCTGGAT+TGG	-	chr5.4:15286200-15286219	MS.gene67555:intron	25.0%
!!	TTGACTAAGATTTTGAATGT+TGG	-	chr5.4:15286666-15286685	MS.gene67555:intron	25.0%
!!	TTGTTTTCTTTTGTGGTTTT+TGG	-	chr5.4:15286040-15286059	MS.gene67555:intron	25.0%
!!!	ATTGTTTTTGTGATAATAGG+AGG	-	chr5.4:15285856-15285875	MS.gene67555:CDS	25.0%
!!!	CAATATTTTAGAACACCATT+TGG	-	chr5.4:15287097-15287116	MS.gene67555:CDS	25.0%
!!!	CATTTGGATTTTAGTTCAAA+TGG	-	chr5.4:15287113-15287132	MS.gene67555:CDS	25.0%
!!!	TCATCGGAATTTTTAGAAAA+AGG	+	chr5.4:15288206-15288225	None:intergenic	25.0%
!!!	TGGTTTTTGGTTTTGATTTA+TGG	-	chr5.4:15286053-15286072	MS.gene67555:intron	25.0%
!!!	TGTGAATTTGTTTTCTTTTG+TGG	-	chr5.4:15286033-15286052	MS.gene67555:intron	25.0%
!!!	TTGTTTTTGTGATAATAGGA+GGG	-	chr5.4:15285857-15285876	MS.gene67555:CDS	25.0%
	AAATCTCAGTTACTTGTCAT+CGG	+	chr5.4:15288222-15288241	None:intergenic	30.0%
	AAATGCTTCCTTTGACATTA+GGG	+	chr5.4:15287300-15287319	None:intergenic	30.0%
	AATCCTATATTTAATCCAGG+AGG	+	chr5.4:15287643-15287662	None:intergenic	30.0%
	ATGCTACATAGTTTCTGATT+TGG	+	chr5.4:15286286-15286305	None:intergenic	30.0%
	CTACTGCAATATAAAGGTTT+AGG	+	chr5.4:15288177-15288196	None:intergenic	30.0%
	GATTAATCAGAATTTGGTTG+TGG	-	chr5.4:15285924-15285943	MS.gene67555:CDS	30.0%
	GTAAGCATTATCCATATTTG+GGG	+	chr5.4:15287734-15287753	None:intergenic	30.0%
	TAAATGCTTCCTTTGACATT+AGG	+	chr5.4:15287301-15287320	None:intergenic	30.0%
	TAAGCATTATCCATATTTGG+GGG	+	chr5.4:15287733-15287752	None:intergenic	30.0%
	TGACATTAATGAAAGGATGA+GGG	-	chr5.4:15287537-15287556	MS.gene67555:CDS	30.0%
	TTGACATTAATGAAAGGATG+AGG	-	chr5.4:15287536-15287555	MS.gene67555:CDS	30.0%
	TTGGCAAAATCAAGTTCAAA+TGG	-	chr5.4:15286725-15286744	MS.gene67555:CDS	30.0%
	TTGTTAACAACACAAGGATA+AGG	+	chr5.4:15285958-15285977	None:intergenic	30.0%
	TTTCATGAGAAGGTTCTTAA+AGG	-	chr5.4:15288316-15288335	MS.gene67555:CDS	30.0%
!	AGTTGTTGACTGCAAATAAT+TGG	-	chr5.4:15286927-15286946	MS.gene67555:intron	30.0%
!	ATTTAATTTCCCCCCAAATA+TGG	-	chr5.4:15287720-15287739	MS.gene67555:intron	30.0%
!	CAAGTCAATAGCATTTTTCA+AGG	+	chr5.4:15287620-15287639	None:intergenic	30.0%
!	GACTAAGATTTTGAATGTTG+GGG	-	chr5.4:15286668-15286687	MS.gene67555:intron	30.0%
!	GCATTGACAATTCAGATTAT+TGG	-	chr5.4:15286302-15286321	MS.gene67555:intron	30.0%
!	GTGTAAAATTTTGTAGGAGA+AGG	-	chr5.4:15288244-15288263	MS.gene67555:CDS	30.0%
!	TTTAACCACATCTGATAATG+TGG	+	chr5.4:15286357-15286376	None:intergenic	30.0%
!!	CAAATAATTGGTCTTATAGG+AGG	-	chr5.4:15286939-15286958	MS.gene67555:intron	30.0%
!!	CTGCAAATAATTGGTCTTAT+AGG	-	chr5.4:15286936-15286955	MS.gene67555:intron	30.0%
!!	GATGATTTAAGTTTCTGGTT+TGG	-	chr5.4:15286161-15286180	MS.gene67555:intron	30.0%
!!	TCATTTTAGCAAAACTTGCA+TGG	+	chr5.4:15286512-15286531	None:intergenic	30.0%
!!!	GGTTTTGATTTATGGTTTGT+GGG	-	chr5.4:15286061-15286080	MS.gene67555:intron	30.0%
!!!	TGGTTTTGATTTATGGTTTG+TGG	-	chr5.4:15286060-15286079	MS.gene67555:intron	30.0%
	AAAACTATGCGCAATTGTGT+TGG	-	chr5.4:15288727-15288746	MS.gene67555:intron	35.0%
	AACTACCAAAAGGTCAAACT+TGG	-	chr5.4:15286706-15286725	MS.gene67555:intron	35.0%
	AAGCATTATCCATATTTGGG+GGG	+	chr5.4:15287732-15287751	None:intergenic	35.0%
	AATCAGAATTTGGTTGTGGA+AGG	-	chr5.4:15285928-15285947	MS.gene67555:CDS	35.0%
	AATTGCTGTGCCATATAGTT+TGG	+	chr5.4:15287517-15287536	None:intergenic	35.0%
	AATTTGTCATGCACCTGAAA+AGG	+	chr5.4:15287772-15287791	None:intergenic	35.0%
	ACCACACTAAAATTTGAAGG+TGG	+	chr5.4:15287252-15287271	None:intergenic	35.0%
	ATATATTCAGCAACTGCAAG+AGG	+	chr5.4:15287030-15287049	None:intergenic	35.0%
	ATGTTGGGGTCAATTTGAAT+AGG	-	chr5.4:15286682-15286701	MS.gene67555:intron	35.0%
	ATTTGTCATGCACCTGAAAA+GGG	+	chr5.4:15287771-15287790	None:intergenic	35.0%
	CACAAAAACATCTAATGCTG+AGG	-	chr5.4:15286589-15286608	MS.gene67555:intron	35.0%
	CAGAAAAGTTGAGGTACATT+TGG	-	chr5.4:15287071-15287090	MS.gene67555:CDS	35.0%
	CTAAAATTTGAAGGTGGATG+TGG	+	chr5.4:15287246-15287265	None:intergenic	35.0%
	CTCTTACTACAGAAAAGTTG+AGG	-	chr5.4:15287062-15287081	MS.gene67555:intron	35.0%
	CTGTGTTTCACCAAACTATA+TGG	-	chr5.4:15287504-15287523	MS.gene67555:CDS	35.0%
	GAACCTTCTCATGAAAACAT+AGG	+	chr5.4:15288312-15288331	None:intergenic	35.0%
	GAATAAGCCTTCGATTTAGT+TGG	+	chr5.4:15288695-15288714	None:intergenic	35.0%
	GCAACCACACTAAAATTTGA+AGG	+	chr5.4:15287255-15287274	None:intergenic	35.0%
	GGGTGTGATTAATCAGAATT+TGG	-	chr5.4:15285918-15285937	MS.gene67555:CDS	35.0%
	GGTGACATTGAAAATCTTTG+TGG	-	chr5.4:15288113-15288132	MS.gene67555:intron	35.0%
	GTATCTACATCTACATGTCA+CGG	-	chr5.4:15286415-15286434	MS.gene67555:CDS	35.0%
	GTGACATGTAGATGTAGATA+CGG	+	chr5.4:15286416-15286435	None:intergenic	35.0%
	TATATTCAGCAACTGCAAGA+GGG	+	chr5.4:15287029-15287048	None:intergenic	35.0%
	TCAAGGTATAGTGATTCAGA+AGG	+	chr5.4:15287603-15287622	None:intergenic	35.0%
	TGTTGGGGTCAATTTGAATA+GGG	-	chr5.4:15286683-15286702	MS.gene67555:intron	35.0%
	TGTTTGTGAGAAGAAACAAG+CGG	-	chr5.4:15286103-15286122	MS.gene67555:intron	35.0%
	TTGAATAGGGAACTACCAAA+AGG	-	chr5.4:15286696-15286715	MS.gene67555:intron	35.0%
	TTGCCTCCTGGATTAAATAT+AGG	-	chr5.4:15287637-15287656	MS.gene67555:intron	35.0%
!	AATGGTTGACTTTCACAAGA+AGG	-	chr5.4:15289128-15289147	MS.gene67555:CDS	35.0%
!	AATTTTGTAGGAGAAGGTGA+TGG	-	chr5.4:15288250-15288269	MS.gene67555:CDS	35.0%
!	ATCCTTGTGTTGTTAACAAG+AGG	-	chr5.4:15285959-15285978	MS.gene67555:intron	35.0%
!	CAGAGAATGCTCTAGTTTAA+TGG	-	chr5.4:15289110-15289129	MS.gene67555:CDS	35.0%
!	GGTTAGATTCTAGAAAGGTT+GGG	-	chr5.4:15285878-15285897	MS.gene67555:CDS	35.0%
!	TCCTTGTGTTGTTAACAAGA+GGG	-	chr5.4:15285960-15285979	MS.gene67555:intron	35.0%
!	TCTAGTTCCAACTAAATCGA+AGG	-	chr5.4:15288685-15288704	MS.gene67555:intron	35.0%
!	TTTTGCCAAGTTTGACCTTT+TGG	+	chr5.4:15286714-15286733	None:intergenic	35.0%
!!	ATTTTTGGAAAAGCAGTCTG+TGG	-	chr5.4:15287840-15287859	MS.gene67555:intron	35.0%
!!	TCCACCTTCAAATTTTAGTG+TGG	-	chr5.4:15287248-15287267	MS.gene67555:CDS	35.0%
	AACACATGTCAGTGTGACAA+TGG	+	chr5.4:15287211-15287230	None:intergenic	40.0%
	ACAGCTCAATGCACTATGTA+TGG	-	chr5.4:15288576-15288595	MS.gene67555:intron	40.0%
	AGAAAGAAGCTTCAGTTGGT+TGG	-	chr5.4:15287865-15287884	MS.gene67555:intron	40.0%
	AGAATTAGATCTCCAACCAC+AGG	+	chr5.4:15287937-15287956	None:intergenic	40.0%
	AGTGGAGTAAGACATGTGAA+TGG	-	chr5.4:15288607-15288626	MS.gene67555:intron	40.0%
	AGTTGAGATGGAGGATATCA+TGG	-	chr5.4:15286963-15286982	MS.gene67555:intron	40.0%
	ATCAATTCCTCACCTAGTGA+TGG	+	chr5.4:15286144-15286163	None:intergenic	40.0%
	CAATGCATAGCGAATCAGAT+CGG	-	chr5.4:15286831-15286850	MS.gene67555:intron	40.0%
	CATGAGAAGGTTCTTAAAGG+CGG	-	chr5.4:15288319-15288338	MS.gene67555:CDS	40.0%
	CATTTCCAGAACTTCTTTCC+GGG	+	chr5.4:15287980-15287999	None:intergenic	40.0%
	CCATTTCCAGAACTTCTTTC+CGG	+	chr5.4:15287981-15288000	None:intergenic	40.0%
	CTCATGAAAACATAGGCTGT+CGG	+	chr5.4:15288305-15288324	None:intergenic	40.0%
	CTTATAGGAGGAAGTTGAGA+TGG	-	chr5.4:15286951-15286970	MS.gene67555:intron	40.0%
	GAAACAATGTCTCATGCATG+AGG	+	chr5.4:15287798-15287817	None:intergenic	40.0%
	GAGATCAAAAGACTATGCCT+CGG	-	chr5.4:15288879-15288898	MS.gene67555:intron	40.0%
	GGTAAGAAAGAAGCTTCAGT+TGG	-	chr5.4:15287861-15287880	MS.gene67555:intron	40.0%
	TATCATGGAAGAGCCTGTTA+TGG	-	chr5.4:15286978-15286997	MS.gene67555:intron	40.0%
	TCAATTCCTCACCTAGTGAT+GGG	+	chr5.4:15286143-15286162	None:intergenic	40.0%
	TCTGCGCTACTGCAATATAA+AGG	+	chr5.4:15288183-15288202	None:intergenic	40.0%
	TTCTCCAGAAGAAAACTTGC+TGG	+	chr5.4:15289228-15289247	None:intergenic	40.0%
	TTGACTTTCACAAGAAGGCT+GGG	-	chr5.4:15289133-15289152	MS.gene67555:CDS	40.0%
!	ACCTTGGTTGTGTTATTGAG+TGG	+	chr5.4:15288062-15288081	None:intergenic	40.0%
!	AGGAGGGTTAGATTCTAGAA+AGG	-	chr5.4:15285873-15285892	MS.gene67555:CDS	40.0%
!	CAGCCTATGTTTTCATGAGA+AGG	-	chr5.4:15288306-15288325	MS.gene67555:CDS	40.0%
!	CTATGTATGGTGTCAAACAG+TGG	-	chr5.4:15288589-15288608	MS.gene67555:intron	40.0%
!	GAGTTGATCTTCAGAGTAGT+TGG	+	chr5.4:15288637-15288656	None:intergenic	40.0%
!	GGGTTAGATTCTAGAAAGGT+TGG	-	chr5.4:15285877-15285896	MS.gene67555:CDS	40.0%
!	GTCACACTGACATGTGTTTT+TGG	-	chr5.4:15287213-15287232	MS.gene67555:CDS	40.0%
!	GTTTTGCATGAACCCTTTTC+AGG	-	chr5.4:15287756-15287775	MS.gene67555:intron	40.0%
!	TGAACCAGCAAGTTTTCTTC+TGG	-	chr5.4:15289221-15289240	MS.gene67555:intron	40.0%
!	TGGTTTAGTGGCAATGCTTT+TGG	-	chr5.4:15287885-15287904	MS.gene67555:intron	40.0%
!!	TCTGTTTGCTCTAAAGCCAT+TGG	+	chr5.4:15286809-15286828	None:intergenic	40.0%
!!!	GGACTGCAGTTTTAAAACCT+TGG	+	chr5.4:15288078-15288097	None:intergenic	40.0%
	AAGAGGGTCATTTGCATCAG+GGG	+	chr5.4:15287013-15287032	None:intergenic	45.0%
	ATACACCCGGAAAGAAGTTC+TGG	-	chr5.4:15287972-15287991	MS.gene67555:intron	45.0%
	ATAGGAGGAAGTTGAGATGG+AGG	-	chr5.4:15286954-15286973	MS.gene67555:intron	45.0%
	ATTTGAAGGTGGATGTGGAC+AGG	+	chr5.4:15287241-15287260	None:intergenic	45.0%
	CAAGAGGGTCATTTGCATCA+GGG	+	chr5.4:15287014-15287033	None:intergenic	45.0%
	GCCACTCAATAACACAACCA+AGG	-	chr5.4:15288058-15288077	MS.gene67555:CDS	45.0%
	GCCCTCTTGTTAACAACACA+AGG	+	chr5.4:15285964-15285983	None:intergenic	45.0%
	TATCAGACAGAGCATACACC+CGG	-	chr5.4:15287959-15287978	MS.gene67555:intron	45.0%
	TGAAAGGATGAGGGCTATAC+TGG	-	chr5.4:15287546-15287565	MS.gene67555:CDS	45.0%
	TGAGAAGAAACAAGCGGATC+CGG	-	chr5.4:15286109-15286128	MS.gene67555:intron	45.0%
	TGGTTGAGCCCTAATGTCAA+AGG	-	chr5.4:15287289-15287308	MS.gene67555:intron	45.0%
	TTGAGCTGTATAGACAGCAG+CGG	+	chr5.4:15288565-15288584	None:intergenic	45.0%
!	ATGGTTTGTGGGTGTATCAC+AGG	-	chr5.4:15286072-15286091	MS.gene67555:intron	45.0%
!	CACATCTGATAATGTGGCGT+CGG	+	chr5.4:15286351-15286370	None:intergenic	45.0%
!	CGATCTGATTCGCTATGCAT+TGG	+	chr5.4:15286833-15286852	None:intergenic	45.0%
!	GCTTTTGGCATGCAAGTATG+AGG	-	chr5.4:15287900-15287919	MS.gene67555:intron	45.0%
!	GGAAACTTACAGGAGCACAT+AGG	-	chr5.4:15289160-15289179	MS.gene67555:CDS	45.0%
!	GTTGACTTTCACAAGAAGGC+TGG	-	chr5.4:15289132-15289151	MS.gene67555:CDS	45.0%
!	TAGCAGCTCAAGCTGTTTCA+TGG	+	chr5.4:15288472-15288491	None:intergenic	45.0%
!	TGATGATGAACACAAGCCAG+TGG	-	chr5.4:15286769-15286788	MS.gene67555:CDS	45.0%
!	TGGGCAAAACAGAAGAGCAT+TGG	-	chr5.4:15285897-15285916	MS.gene67555:CDS	45.0%
!!	AATGCTATTGACTTGCCTCC+TGG	-	chr5.4:15287625-15287644	MS.gene67555:intron	45.0%
!!	ACTGGTTGACTGGCTTATTG+AGG	-	chr5.4:15287564-15287583	MS.gene67555:intron	45.0%
!!	CCGGAAAGAAGTTCTGGAAA+TGG	-	chr5.4:15287978-15287997	MS.gene67555:intron	45.0%
!!	GCTTCAGTTGGTTGGTTTAG+TGG	-	chr5.4:15287873-15287892	MS.gene67555:intron	45.0%
!!	GGGCAAAACAGAAGAGCATT+GGG	-	chr5.4:15285898-15285917	MS.gene67555:CDS	45.0%
!!	ATAATAACTATATAAAAAAA+AGG	+	chr5.4:15289032-15289051	None:intergenic	5.0%
	ACATTAGGGCTCAACCAGAG+CGG	+	chr5.4:15287286-15287305	None:intergenic	50.0%
	CAGTCCTCTTGCAGTTTCTG+TGG	-	chr5.4:15288092-15288111	MS.gene67555:CDS	50.0%
	CGACGCCACATTATCAGATG+TGG	-	chr5.4:15286349-15286368	MS.gene67555:intron	50.0%
	GAAGTTTCAGTGCCTGTGGT+TGG	-	chr5.4:15287922-15287941	MS.gene67555:intron	50.0%
	GCAAGAGGGTCATTTGCATC+AGG	+	chr5.4:15287015-15287034	None:intergenic	50.0%
	GCATAGCGAATCAGATCGGA+TGG	-	chr5.4:15286835-15286854	MS.gene67555:intron	50.0%
	GGTTGCTACATATACCGCTC+TGG	-	chr5.4:15287269-15287288	MS.gene67555:CDS	50.0%
	GTCACCACAGAAACTGCAAG+AGG	+	chr5.4:15288099-15288118	None:intergenic	50.0%
	TGAGGAAGTTTCAGTGCCTG+TGG	-	chr5.4:15287918-15287937	MS.gene67555:intron	50.0%
	TGATGAACACAAGCCAGTGG+AGG	-	chr5.4:15286772-15286791	MS.gene67555:CDS	50.0%
	TGCACGATTCTTCTACTCCG+AGG	+	chr5.4:15288899-15288918	None:intergenic	50.0%
	TTCACAAGAAGGCTGGGACA+GGG	-	chr5.4:15289139-15289158	MS.gene67555:CDS	50.0%
	TTTCACAAGAAGGCTGGGAC+AGG	-	chr5.4:15289138-15289157	MS.gene67555:CDS	50.0%
!	GGGGTGTCAATGTCCATAAC+AGG	+	chr5.4:15286994-15287013	None:intergenic	50.0%
!	TGAGGGCTATACTGGTTGAC+TGG	-	chr5.4:15287554-15287573	MS.gene67555:CDS	50.0%
!!	CTAGTGATGGGTCGATGAAC+CGG	+	chr5.4:15286131-15286150	None:intergenic	50.0%
!!	TGCTCTAAAGCCATTGGCAC+TGG	+	chr5.4:15286803-15286822	None:intergenic	50.0%
	CACGGTGTCTCTGTCATGTC+AGG	-	chr5.4:15286433-15286452	MS.gene67555:CDS	55.0%
	CATCGACCCATCACTAGGTG+AGG	-	chr5.4:15286134-15286153	MS.gene67555:intron	55.0%
	CGGTTCATCGACCCATCACT+AGG	-	chr5.4:15286129-15286148	MS.gene67555:intron	55.0%
	GCTGGGACAGGGAAACTTAC+AGG	-	chr5.4:15289150-15289169	MS.gene67555:CDS	55.0%
	GCAGCGGCAGCTAGTTGAGA+AGG	+	chr5.4:15288549-15288568	None:intergenic	60.0%
	GGAGGATCAGCCAGTGCCAA+TGG	-	chr5.4:15286790-15286809	MS.gene67555:CDS	60.0%
!!	GGCACTGGCTGATCCTCCAC+TGG	+	chr5.4:15286788-15286807	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	15285812	15289257	15285812	ID=MS.gene67555
chr5.4	mRNA	15285812	15289257	15285812	ID=MS.gene67555.t1;Parent=MS.gene67555
chr5.4	exon	15289254	15289257	15289254	ID=MS.gene67555.t1.exon1;Parent=MS.gene67555.t1
chr5.4	CDS	15289254	15289257	15289254	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15289077	15289194	15289077	ID=MS.gene67555.t1.exon2;Parent=MS.gene67555.t1
chr5.4	CDS	15289077	15289194	15289077	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15288919	15288975	15288919	ID=MS.gene67555.t1.exon3;Parent=MS.gene67555.t1
chr5.4	CDS	15288919	15288975	15288919	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15288459	15288504	15288459	ID=MS.gene67555.t1.exon4;Parent=MS.gene67555.t1
chr5.4	CDS	15288459	15288504	15288459	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15288213	15288365	15288213	ID=MS.gene67555.t1.exon5;Parent=MS.gene67555.t1
chr5.4	CDS	15288213	15288365	15288213	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15287986	15288111	15287986	ID=MS.gene67555.t1.exon6;Parent=MS.gene67555.t1
chr5.4	CDS	15287986	15288111	15287986	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15287484	15287573	15287484	ID=MS.gene67555.t1.exon7;Parent=MS.gene67555.t1
chr5.4	CDS	15287484	15287573	15287484	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15287070	15287291	15287070	ID=MS.gene67555.t1.exon8;Parent=MS.gene67555.t1
chr5.4	CDS	15287070	15287291	15287070	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15286711	15286809	15286711	ID=MS.gene67555.t1.exon9;Parent=MS.gene67555.t1
chr5.4	CDS	15286711	15286809	15286711	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15286411	15286589	15286411	ID=MS.gene67555.t1.exon10;Parent=MS.gene67555.t1
chr5.4	CDS	15286411	15286589	15286411	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1
chr5.4	exon	15285812	15285956	15285812	ID=MS.gene67555.t1.exon11;Parent=MS.gene67555.t1
chr5.4	CDS	15285812	15285956	15285812	ID=cds.MS.gene67555.t1;Parent=MS.gene67555.t1

Gene Sequence

>MS.gene67555

ATGGGTTGTTGAATTTTTTAATTTATTAATTAATTGATTAATTAATTGTTTTTGTGATAATAGGAGGGTTAGATTCTAGAAAGGTTGGGCAAAACAGAAGAGCATTGGGTGTGATTAATCAGAATTTGGTTGTGGAAGGACGTCCTTATCCTTGTGTTGTTAACAAGAGGGCATTGTCAGAGTAAATTCTTGATCAGATTATGAACCCTTCTTTTTGTGATTGTGAATTTGTTTTCTTTTGTGGTTTTTGGTTTTGATTTATGGTTTGTGGGTGTATCACAGGAGAAATGATGTTTGTGAGAAGAAACAAGCGGATCCGGTTCATCGACCCATCACTAGGTGAGGAATTGATGATTTAAGTTTCTGGTTTGGTTCATAGACCCTTTAGTGTAATTTGAATTTCTGGATTGGTTGACCATAAGAAAAAACAAAGATATTGAGATAAGTGTTTGAATTTGGCAATTTTAAAAACCAAATCAGAAACTATGTAGCATTGACAATTCAGATTATTGGCATGTCTAGTGTTCGACAGATGTCCGACGCCACATTATCAGATGTGGTTAAATTCAATTACTATTATTTTCTTGAATTAATTTGTCATCCGTATCTACATCTACATGTCACGGTGTCTCTGTCATGTCAGGTGTCTGTGTCAGTGCGTTATAGATCAGAAATAATAGAATTTTTCATATTATTTCCATGCAAGTTTTGCTAAAATGATGATGGACTTATCTTGATTTACTGTTATATTAGGAGGTTTGCTGCAAAGATTGCTAGCACAAAAACATCTAATGCTGAGGTAGAATCTTCTATTCCTTTGTGAAATTTTGCTACATTGTACAGATTCTTACCTTTTGACTAAGATTTTGAATGTTGGGGTCAATTTGAATAGGGAACTACCAAAAGGTCAAACTTGGCAAAATCAAGTTCAAATGGATTTGGAGATTTCATATTTGTTGATGATGAACACAAGCCAGTGGAGGATCAGCCAGTGCCAATGGCTTTAGAGCAAACAGAACCAATGCATAGCGAATCAGATCGGATGGTAATTCTTTAATGTCCTGTTTATTTAATTAGTTAATAAGTTCAAATTTTAAATCCTTTTTTTAAATTTTAGTTGTTGACTGCAAATAATTGGTCTTATAGGAGGAAGTTGAGATGGAGGATATCATGGAAGAGCCTGTTATGGACATTGACACCCCTGATGCAAATGACCCTCTTGCAGTTGCTGAATATATTGAAGATCTTTACTCTTACTACAGAAAAGTTGAGGTACATTTGGAATCAATATTTTAGAACACCATTTGGATTTTAGTTCAAATGGAATATATGAAGCAATGACTTAGACATCTATAATAATTTGAGAAAAAACAATAATTGAATGTAATGGTGTGTGCCATTGTCACACTGACATGTGTTTTTGGCACCTGTCCACATCCACCTTCAAATTTTAGTGTGGTTGCTACATATACCGCTCTGGTTGAGCCCTAATGTCAAAGGAAGCATTTATTGTATTTTCTCTTTTATTATAGCTTATTTCACTGTATTTAATCAGCATGAAACCAAATTTATACTCTTGAATTTTCACTTTTATAGTTTTATGTAAATTGATTTCAGAATTCCAATTAATATGGCAATTCTTTCCAATCAAATTTCAATAACAAATAGCATTAACTACATGCAGAGTACTAGCTGTGTTTCACCAAACTATATGGCACAGCAATTTGACATTAATGAAAGGATGAGGGCTATACTGGTTGACTGGCTTATTGAGGTATATTCAATGTTCCTTCTGAATCACTATACCTTGAAAAATGCTATTGACTTGCCTCCTGGATTAAATATAGGATTAATTATGTCTAAAAGCATAATTAACTCTTAAATATAATTATCATTAACTTTTAGTAAATTTAATTTCCCCCCAAATATGGATAATGCTTACTTGTTTTGCATGAACCCTTTTCAGGTGCATGACAAATTCGACCTCATGCATGAGACATTGTTTCTCACTGTCAATCTTATAGACAGATTTTTGGAAAAGCAGTCTGTGGTAAGAAAGAAGCTTCAGTTGGTTGGTTTAGTGGCAATGCTTTTGGCATGCAAGTATGAGGAAGTTTCAGTGCCTGTGGTTGGAGATCTAATTCTTATATCAGACAGAGCATACACCCGGAAAGAAGTTCTGGAAATGGTAACATTTCTTTGCTTGCTTATTGTGTCAGAACCTTTACATTTATAGCATTTGTGGAGCCACTCAATAACACAACCAAGGTTTTAAAACTGCAGTCCTCTTGCAGTTTCTGTGGTGACATTGAAAATCTTTGTGGTAGCGCAGACTTCTTTGCGACTGCAGAGACATAAAAAACCTAAACCTTTATATTGCAGTAGCGCAGACCTTTTTCTAAAAATTCCGATGACAAGTAACTGAGATTTGTGTGTAAAATTTTGTAGGAGAAGGTGATGGTCAATGCATTGAAGTTTAACATATCTGTGCCGACAGCCTATGTTTTCATGAGAAGGTTCTTAAAGGCGGCTCAAGCAGACAGAAAAGTAAGCACTCAAATTTCTGCCTAATATATTTCCATGCCATTTTTTTTTTCTTTATCATAACTAGTAATACCATAGTTACTAATTACAAATACTTTTTGCTATATTGCTCACCATGAAACAGCTTGAGCTGCTAGCTTTCTTCTTGATTGAGCTATCTCTTGTAGAATATGCAATGTTGAAGTTCTCTCCTTCTCAACTAGCTGCCGCTGCTGTCTATACAGCTCAATGCACTATGTATGGTGTCAAACAGTGGAGTAAGACATGTGAATGGCACACCAACTACTCTGAAGATCAACTCTTGTAAGCATTTTAAAGTTCAGTCTTTTTCTAGTTCCAACTAAATCGAAGGCTTATTCATTATGAGTTCAAAAACTATGCGCAATTGTGTTGGTCTATAAAAATTGAACATTCAATCATATCACACATGTAGATATTTGTTGAGAAATATAACAAAGCTAGACATCATTATGAGTTATGATATGATTAGATGTACGTGTAAACTACTAACTAGTAATTCTTTGAGATCAAAAGACTATGCCTCGGAGTAGAAGAATCGTGCATTCAATCTTATCACACATGTTGATATTTGTTGAGAGATATAACAAAATGACATCATAGGGAGATACGATTAGATGCATCTGTAGATAATTTTACAAAATCAGTGCCATAACCTTTTTTTTATATAGTTATTATGAAATGTATGCTAAATAAATACTAAATAGTGACTGCTTTGTTTGTGACTACTTACTTACAGAGAATGCTCTAGTTTAATGGTTGACTTTCACAAGAAGGCTGGGACAGGGAAACTTACAGGAGCACATAGGAAGTATTGCACATCAAAATTTAGCTATACTGCGAAATGTGAACCAGCAAGTTTTCTTCTGGAGAACGAGTTGTAG

Protein sequence

>MS.gene67555.t1

MGGLDSRKVGQNRRALGVINQNLVVEGRPYPCVVNKRALSERNDVCEKKQADPVHRPITRRFAAKIASTKTSNAEGTTKRSNLAKSSSNGFGDFIFVDDEHKPVEDQPVPMALEQTEPMHSESDRMEEVEMEDIMEEPVMDIDTPDANDPLAVAEYIEDLYSYYRKVESTSCVSPNYMAQQFDINERMRAILVDWLIEVHDKFDLMHETLFLTVNLIDRFLEKQSVVRKKLQLVGLVAMLLACKYEEVSVPVVGDLILISDRAYTRKEVLEMEKVMVNALKFNISVPTAYVFMRRFLKAAQADRKLELLAFFLIELSLVEYAMLKFSPSQLAAAAVYTAQCTMYGVKQWSKTCEWHTNYSEDQLLECSSLMVDFHKKAGTGKLTGAHRKYCTSKFSYTAKCEPASFLLENEL