AlfalfaGEDB | MS.gene81280

M. sativa cultivar XinJiangDaYe / MS.gene81280

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81280.t1	XP_003617884.1	100	117	0	0	1	117	30	146	8.50E-56	226.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81280.t1	P51414	87.2	117	15	0	1	117	30	146	1.5e-53	209.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81280.t1	G7K4S3	100.0	117	0	0	1	117	30	146	6.1e-56	226.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene81280	0.839918	1.19E-57	-1.69E-46
MS.gene049341	MS.gene81280	0.811641	6.32E-51	-1.69E-46
MS.gene049411	MS.gene81280	0.803557	3.29E-49	-1.69E-46
MS.gene049821	MS.gene81280	0.811583	6.51E-51	-1.69E-46
MS.gene049887	MS.gene81280	0.833475	5.22E-56	-1.69E-46
MS.gene050277	MS.gene81280	0.828799	7.35E-55	-1.69E-46
MS.gene050689	MS.gene81280	0.835894	1.29E-56	-1.69E-46
MS.gene050752	MS.gene81280	0.835603	1.52E-56	-1.69E-46
MS.gene050838	MS.gene81280	0.908714	1.26E-81	-1.69E-46
MS.gene050955	MS.gene81280	0.809712	1.65E-50	-1.69E-46
MS.gene051003	MS.gene81280	0.854857	9.31E-62	-1.69E-46
MS.gene051233	MS.gene81280	0.834154	3.53E-56	-1.69E-46
MS.gene051234	MS.gene81280	0.858733	6.73E-63	-1.69E-46
MS.gene051328	MS.gene81280	0.86202	6.81E-64	-1.69E-46
MS.gene051343	MS.gene81280	0.823796	1.14E-53	-1.69E-46
MS.gene05134	MS.gene81280	0.800825	1.20E-48	-1.69E-46
MS.gene051700	MS.gene81280	0.824884	6.34E-54	-1.69E-46
MS.gene051746	MS.gene81280	0.845924	3.00E-59	-1.69E-46
MS.gene051748	MS.gene81280	0.84829	6.73E-60	-1.69E-46
MS.gene051850	MS.gene81280	0.881339	2.61E-70	-1.69E-46
MS.gene051885	MS.gene81280	0.822449	2.35E-53	-1.69E-46
MS.gene05188	MS.gene81280	0.847598	1.04E-59	-1.69E-46
MS.gene051903	MS.gene81280	0.852363	4.85E-61	-1.69E-46
MS.gene051975	MS.gene81280	0.830739	2.48E-55	-1.69E-46
MS.gene052133	MS.gene81280	0.858648	7.13E-63	-1.69E-46
MS.gene052335	MS.gene81280	0.849502	3.10E-60	-1.69E-46
MS.gene052342	MS.gene81280	0.82224	2.63E-53	-1.69E-46
MS.gene052397	MS.gene81280	0.813525	2.45E-51	-1.69E-46
MS.gene052947	MS.gene81280	0.884932	1.26E-71	-1.69E-46
MS.gene053058	MS.gene81280	0.853301	2.62E-61	-1.69E-46
MS.gene053109	MS.gene81280	0.815567	8.65E-52	-1.69E-46
MS.gene053122	MS.gene81280	0.819239	1.29E-52	-1.69E-46
MS.gene053564	MS.gene81280	0.813977	1.95E-51	-1.69E-46
MS.gene053581	MS.gene81280	0.844272	8.38E-59	-1.69E-46
MS.gene053952	MS.gene81280	0.818091	2.35E-52	-1.69E-46
MS.gene054403	MS.gene81280	0.834731	2.53E-56	-1.69E-46
MS.gene055424	MS.gene81280	0.829087	6.26E-55	-1.69E-46
MS.gene055465	MS.gene81280	0.8201	8.20E-53	-1.69E-46
MS.gene055466	MS.gene81280	0.83067	2.58E-55	-1.69E-46
MS.gene055468	MS.gene81280	0.855543	5.88E-62	-1.69E-46
MS.gene055469	MS.gene81280	0.816345	5.80E-52	-1.69E-46
MS.gene055513	MS.gene81280	0.813509	2.47E-51	-1.69E-46
MS.gene055580	MS.gene81280	-0.850763	1.38E-60	-1.69E-46
MS.gene055808	MS.gene81280	0.804033	2.62E-49	-1.69E-46
MS.gene055979	MS.gene81280	0.821311	4.32E-53	-1.69E-46
MS.gene056043	MS.gene81280	-0.8636	2.22E-64	-1.69E-46
MS.gene056075	MS.gene81280	0.854363	1.29E-61	-1.69E-46
MS.gene056091	MS.gene81280	0.80023	1.59E-48	-1.69E-46
MS.gene056215	MS.gene81280	0.840939	6.43E-58	-1.69E-46
MS.gene056432	MS.gene81280	0.818059	2.39E-52	-1.69E-46
MS.gene056580	MS.gene81280	0.826937	2.06E-54	-1.69E-46
MS.gene056631	MS.gene81280	0.843355	1.47E-58	-1.69E-46
MS.gene056801	MS.gene81280	0.802843	4.63E-49	-1.69E-46
MS.gene056986	MS.gene81280	0.807867	4.10E-50	-1.69E-46
MS.gene057057	MS.gene81280	0.826274	2.97E-54	-1.69E-46
MS.gene057094	MS.gene81280	0.879253	1.45E-69	-1.69E-46
MS.gene057234	MS.gene81280	0.811337	7.36E-51	-1.69E-46
MS.gene057705	MS.gene81280	0.865897	4.23E-65	-1.69E-46
MS.gene05781	MS.gene81280	0.811369	7.25E-51	-1.69E-46
MS.gene058054	MS.gene81280	0.800723	1.26E-48	-1.69E-46
MS.gene058055	MS.gene81280	0.802567	5.28E-49	-1.69E-46
MS.gene058084	MS.gene81280	0.806729	7.14E-50	-1.69E-46
MS.gene058238	MS.gene81280	0.806592	7.63E-50	-1.69E-46
MS.gene058261	MS.gene81280	0.807125	5.89E-50	-1.69E-46
MS.gene058364	MS.gene81280	0.816641	4.98E-52	-1.69E-46
MS.gene05870	MS.gene81280	0.800632	1.32E-48	-1.69E-46
MS.gene05880	MS.gene81280	0.806703	7.23E-50	-1.69E-46
MS.gene058852	MS.gene81280	0.802992	4.31E-49	-1.69E-46
MS.gene058906	MS.gene81280	0.836439	9.35E-57	-1.69E-46
MS.gene059146	MS.gene81280	0.826756	2.28E-54	-1.69E-46
MS.gene059171	MS.gene81280	0.811035	8.56E-51	-1.69E-46
MS.gene059227	MS.gene81280	0.822709	2.05E-53	-1.69E-46
MS.gene059726	MS.gene81280	0.818569	1.83E-52	-1.69E-46
MS.gene05978	MS.gene81280	0.803602	3.22E-49	-1.69E-46
MS.gene06001	MS.gene81280	0.884002	2.78E-71	-1.69E-46
MS.gene06011	MS.gene81280	0.84448	7.37E-59	-1.69E-46
MS.gene060194	MS.gene81280	0.804938	1.70E-49	-1.69E-46
MS.gene060429	MS.gene81280	0.858821	6.33E-63	-1.69E-46
MS.gene060463	MS.gene81280	0.804823	1.80E-49	-1.69E-46
MS.gene060465	MS.gene81280	0.806659	7.39E-50	-1.69E-46
MS.gene060587	MS.gene81280	0.823819	1.13E-53	-1.69E-46
MS.gene060763	MS.gene81280	0.829074	6.31E-55	-1.69E-46
MS.gene060917	MS.gene81280	0.811427	7.04E-51	-1.69E-46
MS.gene061006	MS.gene81280	0.834135	3.57E-56	-1.69E-46
MS.gene061100	MS.gene81280	0.820661	6.10E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81280.t1	MTR_5g096540	100.000	117	0	0	1	117	30	146	4.34e-81	234
MS.gene81280.t1	MTR_5g015570	91.453	117	10	0	1	117	30	146	4.57e-75	219
MS.gene81280.t1	MTR_2g012450	90.598	117	11	0	1	117	30	146	1.72e-74	218
MS.gene81280.t1	MTR_4g126220	86.275	51	7	0	1	51	120	170	2.15e-26	97.1
MS.gene81280.t1	MTR_8g073140	54.651	86	12	1	1	86	1	59	6.27e-22	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81280.t1	AT3G49910	87.179	117	15	0	1	117	30	146	1.27e-74	218
MS.gene81280.t1	AT5G67510	84.615	117	18	0	1	117	30	146	3.41e-71	209

Find 26 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGGAACGGACGTTGTATT+TGG	0.245673	5.2:+80936439	None:intergenic
ATTACTCGTGAGAAAGTTAA+TGG	0.246639	5.2:-80936308	MS.gene81280:CDS
AAGGAGAAGGGTACTAAGTT+TGG	0.353717	5.2:-80936170	MS.gene81280:CDS
GATGAAGTTCAAGTTGTTAG+AGG	0.371888	5.2:-80936401	MS.gene81280:CDS
TGTTGTTACTAAGCTGAGAT+TGG	0.397665	5.2:-80936249	MS.gene81280:CDS
AGGACGTGCTGCTGCGGATA+AGG	0.407566	5.2:-80936189	MS.gene81280:CDS
ACTTCATCGTCTTTGCGAAC+TGG	0.423245	5.2:+80936416	None:intergenic
ATCGTCTTTGCGAACTGGTA+TGG	0.437342	5.2:+80936421	None:intergenic
TACTAAGCTGAGATTGGATA+AGG	0.440322	5.2:-80936243	MS.gene81280:CDS
CAGCTTAGTAACAACAACTT+TGG	0.443241	5.2:+80936256	None:intergenic
AATGGTTCTACTGTTAATGT+TGG	0.493059	5.2:-80936290	MS.gene81280:CDS
GTTGTATTTGGATCGGAGAT+CGG	0.508778	5.2:+80936451	None:intergenic
TTAGTAACAACAACTTTGGA+AGG	0.516252	5.2:+80936260	None:intergenic
GAAGTCGTTGCTTGAGAGGA+AGG	0.531193	5.2:-80936216	MS.gene81280:CDS
GTTGTTAGAGGAACATTCAA+AGG	0.548391	5.2:-80936389	MS.gene81280:CDS
ATAGGAAGTCGTTGCTTGAG+AGG	0.550235	5.2:-80936220	MS.gene81280:CDS
AGCTGAGATTGGATAAGGAT+AGG	0.551181	5.2:-80936238	MS.gene81280:CDS
TGCTGCTGCGGATAAGGAGA+AGG	0.551652	5.2:-80936183	MS.gene81280:CDS
GGCGAAAGGACGTGCTGCTG+CGG	0.551879	5.2:-80936195	MS.gene81280:CDS
AACGGACGTTGTATTTGGAT+CGG	0.584986	5.2:+80936444	None:intergenic
GGAACATTCAAAGGTCGTGA+AGG	0.623758	5.2:-80936380	MS.gene81280:CDS
TTGCTTGAGAGGAAGGCGAA+AGG	0.623866	5.2:-80936209	MS.gene81280:CDS
GTATTTGGATCGGAGATCGG+TGG	0.651925	5.2:+80936454	None:intergenic
GCTGCTGCGGATAAGGAGAA+GGG	0.652585	5.2:-80936182	MS.gene81280:CDS
GATCGGAGATCGGTGGACAG+TGG	0.656057	5.2:+80936461	None:intergenic
CTTTGCGAACTGGTATGGAA+CGG	0.671113	5.2:+80936426	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAAGTTTATCGCAAAAAAT+GGG	-	chr5.2:80936261-80936280	MS.gene81280:CDS	25.0%
!!	TTCAAGTTTATCGCAAAAAA+TGG	-	chr5.2:80936260-80936279	MS.gene81280:CDS	25.0%
	ATTACTCGTGAGAAAGTTAA+TGG	-	chr5.2:80936301-80936320	MS.gene81280:CDS	30.0%
	TTAGTAACAACAACTTTGGA+AGG	+	chr5.2:80936352-80936371	None:intergenic	30.0%
!	AATGGTTCTACTGTTAATGT+TGG	-	chr5.2:80936319-80936338	MS.gene81280:CDS	30.0%
	CAGCTTAGTAACAACAACTT+TGG	+	chr5.2:80936356-80936375	None:intergenic	35.0%
	GTTGTTAGAGGAACATTCAA+AGG	-	chr5.2:80936220-80936239	MS.gene81280:CDS	35.0%
	TACTAAGCTGAGATTGGATA+AGG	-	chr5.2:80936366-80936385	MS.gene81280:CDS	35.0%
	TGTTGTTACTAAGCTGAGAT+TGG	-	chr5.2:80936360-80936379	MS.gene81280:CDS	35.0%
!!	GATGAAGTTCAAGTTGTTAG+AGG	-	chr5.2:80936208-80936227	MS.gene81280:CDS	35.0%
	AACGGACGTTGTATTTGGAT+CGG	+	chr5.2:80936168-80936187	None:intergenic	40.0%
	AGCTGAGATTGGATAAGGAT+AGG	-	chr5.2:80936371-80936390	MS.gene81280:CDS	40.0%
	GTTGTATTTGGATCGGAGAT+CGG	+	chr5.2:80936161-80936180	None:intergenic	40.0%
	TATGGAACGGACGTTGTATT+TGG	+	chr5.2:80936173-80936192	None:intergenic	40.0%
!!	AAGGAGAAGGGTACTAAGTT+TGG	-	chr5.2:80936439-80936458	MS.gene81280:CDS	40.0%
	ACTTCATCGTCTTTGCGAAC+TGG	+	chr5.2:80936196-80936215	None:intergenic	45.0%
	ATAGGAAGTCGTTGCTTGAG+AGG	-	chr5.2:80936389-80936408	MS.gene81280:CDS	45.0%
	ATCGTCTTTGCGAACTGGTA+TGG	+	chr5.2:80936191-80936210	None:intergenic	45.0%
	CTTTGCGAACTGGTATGGAA+CGG	+	chr5.2:80936186-80936205	None:intergenic	45.0%
	GGAACATTCAAAGGTCGTGA+AGG	-	chr5.2:80936229-80936248	MS.gene81280:CDS	45.0%
	GAAGTCGTTGCTTGAGAGGA+AGG	-	chr5.2:80936393-80936412	MS.gene81280:CDS	50.0%
	GTATTTGGATCGGAGATCGG+TGG	+	chr5.2:80936158-80936177	None:intergenic	50.0%
	TTGCTTGAGAGGAAGGCGAA+AGG	-	chr5.2:80936400-80936419	MS.gene81280:CDS	50.0%
	GCTGCTGCGGATAAGGAGAA+GGG	-	chr5.2:80936427-80936446	MS.gene81280:CDS	55.0%
	TGCTGCTGCGGATAAGGAGA+AGG	-	chr5.2:80936426-80936445	MS.gene81280:CDS	55.0%
	GATCGGAGATCGGTGGACAG+TGG	+	chr5.2:80936151-80936170	None:intergenic	60.0%
!	AGGACGTGCTGCTGCGGATA+AGG	-	chr5.2:80936420-80936439	MS.gene81280:CDS	60.0%
!	GGCGAAAGGACGTGCTGCTG+CGG	-	chr5.2:80936414-80936433	MS.gene81280:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	80936139	80936492	80936139	ID=MS.gene81280
chr5.2	mRNA	80936139	80936492	80936139	ID=MS.gene81280.t1;Parent=MS.gene81280
chr5.2	exon	80936139	80936492	80936139	ID=MS.gene81280.t1.exon1;Parent=MS.gene81280.t1
chr5.2	CDS	80936139	80936492	80936139	ID=cds.MS.gene81280.t1;Parent=MS.gene81280.t1

Gene Sequence	>MS.gene81280 ATGAGCGCACCACTGTCCACCGATCTCCGATCCAAATACAACGTCCGTTCCATACCAGTTCGCAAAGACGATGAAGTTCAAGTTGTTAGAGGAACATTCAAAGGTCGTGAAGGTAAAATCGTTCAAGTTTATCGCAAAAAATGGGTTATTCATATTGAACGTATTACTCGTGAGAAAGTTAATGGTTCTACTGTTAATGTTGGCGTTGATCCTTCCAAAGTTGTTGTTACTAAGCTGAGATTGGATAAGGATAGGAAGTCGTTGCTTGAGAGGAAGGCGAAAGGACGTGCTGCTGCGGATAAGGAGAAGGGTACTAAGTTTGGTGCTGAAGATATTATGCAGAATGTTGATTGA
Protein sequence	>MS.gene81280.t1 MSAPLSTDLRSKYNVRSIPVRKDDEVQVVRGTFKGREGKIVQVYRKKWVIHIERITREKVNGSTVNVGVDPSKVVVTKLRLDKDRKSLLERKAKGRAAADKEKGTKFGAEDIMQNVD