AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85253


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85253.t1 MTR_2g099410 96.316 190 7 0 1 190 47 236 1.06e-129 364
MS.gene85253.t1 MTR_4g064917 34.270 178 105 3 2 176 76 244 1.28e-24 97.4
MS.gene85253.t1 MTR_1g031110 27.160 162 109 2 3 161 36 191 8.74e-19 81.3
MS.gene85253.t1 MTR_3g079890 27.160 162 109 2 3 161 40 195 1.35e-18 80.9
MS.gene85253.t1 MTR_1g068960 34.444 90 59 0 79 168 485 574 1.61e-12 65.9
MS.gene85253.t1 MTR_3g114700 29.661 118 82 1 36 153 243 359 1.94e-11 62.4
MS.gene85253.t1 MTR_8g103480 28.947 114 77 2 43 156 49 158 1.95e-11 62.4
MS.gene85253.t1 MTR_7g103600 30.597 134 92 1 27 160 484 616 5.27e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85253.t1 AT3G46870 34.091 176 104 3 2 174 72 238 6.56e-23 92.8
MS.gene85253.t1 AT3G46870 31.915 188 104 4 2 174 72 250 1.60e-19 84.0
MS.gene85253.t1 AT5G09315 28.571 203 120 4 2 185 54 250 1.18e-18 81.6
MS.gene85253.t1 AT1G62350 27.160 162 109 2 3 161 1 156 3.95e-14 68.2
MS.gene85253.t1 AT1G62350 27.160 162 109 2 3 161 57 212 1.29e-13 67.8
MS.gene85253.t1 AT3G04760 27.879 165 112 2 19 180 172 332 3.77e-12 64.7
MS.gene85253.t1 AT1G63330 28.378 148 95 4 34 178 284 423 7.28e-11 60.8
MS.gene85253.t1 AT1G63330 28.378 148 95 4 34 178 284 423 7.28e-11 60.8
MS.gene85253.t1 AT1G63330 28.378 148 95 4 34 178 284 423 7.28e-11 60.8

Find 43 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAACTTGAACGCTTTCTT+TGG 0.235310 2.4:-4795443 None:intergenic
ACTTTCTGAACAAATTTATC+AGG 0.290581 2.4:-4796951 None:intergenic
TACAGATCCATGAAGGCTTC+AGG 0.306225 2.4:+4796816 MS.gene85253:CDS
TCATTGAACAACCTCGTATC+TGG 0.321501 2.4:-4796741 None:intergenic
CAAACACCCTGAAGCCTTCA+TGG 0.358156 2.4:-4796823 None:intergenic
ATTAGGAATCTAATGAATAA+TGG 0.372286 2.4:+4796873 MS.gene85253:CDS
TGACATTCACGATACTGATT+AGG 0.383930 2.4:+4796856 MS.gene85253:CDS
TTCTGACACTATATTGTTGC+TGG 0.386533 2.4:+4796653 MS.gene85253:CDS
TTCAATCAAGTTGTGGAAAA+GGG 0.393035 2.4:+4796711 MS.gene85253:CDS
GAAGGCTTCAGGGTGTTTGC+CGG 0.397835 2.4:+4796827 MS.gene85253:CDS
GGTTGTTCAATGAGATGATC+GGG 0.409297 2.4:+4796751 MS.gene85253:CDS
GCCAAGACAATTAAAACATC+AGG 0.410998 2.4:-4795544 None:intergenic
GTTCAATCAAGTTGTGGAAA+AGG 0.412536 2.4:+4796710 MS.gene85253:CDS
AGGAAGCTGGGTATGATAAA+TGG 0.414897 2.4:+4796616 MS.gene85253:CDS
TAAACTTGTAGTGTCTTGTA+TGG 0.432276 2.4:+4797095 MS.gene85253:intron
TTATTTGCAAGTTGGTAATA+CGG 0.445470 2.4:+4796776 MS.gene85253:CDS
TTCTCAAGTCTAAATTGCTT+AGG 0.453872 2.4:+4795512 MS.gene85253:CDS
GCAATTTAGACTTGAGAACT+TGG 0.462867 2.4:-4795507 None:intergenic
GAAAACTCACATGTTTCAGC+TGG 0.472944 2.4:-4796974 None:intergenic
ACAGATCCATGAAGGCTTCA+GGG 0.478380 2.4:+4796817 MS.gene85253:CDS
ATCATCAGTGATGAGGAAGC+TGG 0.489732 2.4:+4796603 MS.gene85253:CDS
ATCGTGAATGTCAATGAATC+CGG 0.495421 2.4:-4796846 None:intergenic
ATCGGGGTTTATTTGCAAGT+TGG 0.501108 2.4:+4796768 MS.gene85253:CDS
GAATAATGGAGAACATGAAC+TGG 0.504471 2.4:+4796887 MS.gene85253:CDS
GTCTTGTATGGGAAGAGTAA+TGG 0.515604 2.4:+4797107 MS.gene85253:CDS
CCTTGAGAGATAAATGGAGT+TGG 0.528634 2.4:-4795585 None:intergenic
AGGTTGTTCAATGAGATGAT+CGG 0.544532 2.4:+4796750 MS.gene85253:CDS
TCTGACACTATATTGTTGCT+GGG 0.554494 2.4:+4796654 MS.gene85253:CDS
CCAACTCCATTTATCTCTCA+AGG 0.557133 2.4:+4795585 MS.gene85253:CDS
TGGTAATACGGAGAAGGCGA+TGG 0.560110 2.4:+4796788 MS.gene85253:CDS
GCAAGTTGGTAATACGGAGA+AGG 0.574234 2.4:+4796782 MS.gene85253:CDS
CGAGTTTATCATCAGTGATG+AGG 0.580481 2.4:+4796596 MS.gene85253:CDS
TCATCAGTGATGAGGAAGCT+GGG 0.584407 2.4:+4796604 MS.gene85253:CDS
AGAGATGTTCAATCAAGTTG+TGG 0.585094 2.4:+4796704 MS.gene85253:CDS
GGAGGTTTACAGATCCATGA+AGG 0.589588 2.4:+4796809 MS.gene85253:CDS
TCAATCAAGTTGTGGAAAAG+GGG 0.591104 2.4:+4796712 MS.gene85253:CDS
AAAGAACAAGATGATTGAGA+TGG 0.594568 2.4:+4796677 MS.gene85253:CDS
GAACAAGATGATTGAGATGG+CGG 0.602226 2.4:+4796680 MS.gene85253:CDS
TAATACGGAGAAGGCGATGG+AGG 0.641911 2.4:+4796791 MS.gene85253:CDS
AAACTTGTAGTGTCTTGTAT+GGG 0.642469 2.4:+4797096 MS.gene85253:intron
AATATAGTGTCAGAATACAA+TGG 0.656425 2.4:-4796645 None:intergenic
GTTGTTCAATGAGATGATCG+GGG 0.665655 2.4:+4796752 MS.gene85253:CDS
AGGGGTTGAAACCAGATACG+AGG 0.767064 2.4:+4796730 MS.gene85253:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACTTATATGAAAATTATA+TGG + chr2.4:4795822-4795841 MS.gene85253:intron 10.0%
!! ATAAGATAATAGTTTGATAT+TGG - chr2.4:4795940-4795959 None:intergenic 15.0%
!! ATTAGGAATCTAATGAATAA+TGG + chr2.4:4796873-4796892 MS.gene85253:CDS 20.0%
!!! ATGTGAGTTTTCAAATTTTA+TGG + chr2.4:4796985-4797004 MS.gene85253:intron 20.0%
!!! GATATCATTTTCTATAAGAA+CGG - chr2.4:4796166-4796185 None:intergenic 20.0%
!!! GCTTTTTTCATAAATTATTC+TGG + chr2.4:4795969-4795988 MS.gene85253:intron 20.0%
!!! TGATTTTTTAAGTGACATTT+TGG + chr2.4:4797042-4797061 MS.gene85253:intron 20.0%
! AATAATTTGATCGTAGTTGA+AGG + chr2.4:4796264-4796283 MS.gene85253:intron 25.0%
! AATATAGTGTCAGAATACAA+TGG - chr2.4:4796648-4796667 None:intergenic 25.0%
! ACTTTCTGAACAAATTTATC+AGG - chr2.4:4796954-4796973 None:intergenic 25.0%
! GATTAAAGGTCTTAAGAATT+AGG + chr2.4:4796326-4796345 MS.gene85253:intron 25.0%
! GCTAGAAAATTATTTCATCT+AGG + chr2.4:4796219-4796238 MS.gene85253:intron 25.0%
! TAGTTAGAATTATTAGCTAC+TGG - chr2.4:4796518-4796537 None:intergenic 25.0%
!! GCATTTGATAATTGTAACAT+AGG + chr2.4:4796569-4796588 MS.gene85253:intron 25.0%
!! TTTTTACCTTGAGAGATAAA+TGG - chr2.4:4795594-4795613 None:intergenic 25.0%
!!! ACTGTTTTCTCTAGTTTTTT+TGG + chr2.4:4796038-4796057 MS.gene85253:intron 25.0%
!!! CTATTTTCTCAAAGTATTAC+CGG + chr2.4:4796406-4796425 MS.gene85253:intron 25.0%
!!! TTATTTGCAAGTTGGTAATA+CGG + chr2.4:4796776-4796795 MS.gene85253:CDS 25.0%
!!! TTGTTTCTAATAAGCTATCT+AGG + chr2.4:4795774-4795793 MS.gene85253:intron 25.0%
AAACTTGTAGTGTCTTGTAT+GGG + chr2.4:4797096-4797115 MS.gene85253:intron 30.0%
AAAGAACAAGATGATTGAGA+TGG + chr2.4:4796677-4796696 MS.gene85253:CDS 30.0%
AATAACTTGAACGCTTTCTT+TGG - chr2.4:4795446-4795465 None:intergenic 30.0%
AATAAGCTGAAAACTACTCA+CGG + chr2.4:4796004-4796023 MS.gene85253:intron 30.0%
ACAAGTTGCAAACAACTTAT+AGG - chr2.4:4795758-4795777 None:intergenic 30.0%
AGTTAGTCACTAGTGTAATT+TGG - chr2.4:4796121-4796140 None:intergenic 30.0%
ATCACAATTAAAACCCTGTT+TGG + chr2.4:4795613-4795632 MS.gene85253:intron 30.0%
ATTAAAACCCTGTTTGGTTA+AGG + chr2.4:4795619-4795638 MS.gene85253:intron 30.0%
CAACAGAACTTCTAATTAGT+TGG - chr2.4:4796188-4796207 None:intergenic 30.0%
GTCTTAAGAATTAGGATTGA+AGG + chr2.4:4796334-4796353 MS.gene85253:intron 30.0%
GTTAGTCACTAGTGTAATTT+GGG - chr2.4:4796120-4796139 None:intergenic 30.0%
TAAACTTGTAGTGTCTTGTA+TGG + chr2.4:4797095-4797114 MS.gene85253:intron 30.0%
TTCTAATAAGCTATCTAGGA+CGG + chr2.4:4795778-4795797 MS.gene85253:intron 30.0%
TTCTCAAGTCTAAATTGCTT+AGG + chr2.4:4795512-4795531 MS.gene85253:CDS 30.0%
! AACTTGGTTGAGTTTTTCTT+CGG - chr2.4:4795494-4795513 None:intergenic 30.0%
! ACCTTTAATCTTTCAAGTGT+CGG - chr2.4:4796316-4796335 None:intergenic 30.0%
! CGGTAATACTTTGAGAAAAT+AGG - chr2.4:4796408-4796427 None:intergenic 30.0%
! TTCAATCAAGTTGTGGAAAA+GGG + chr2.4:4796711-4796730 MS.gene85253:CDS 30.0%
!! AGTTTGATATTGGCTTTGTT+TGG - chr2.4:4795930-4795949 None:intergenic 30.0%
!! ATATTGGCTTTGTTTGGAAT+TGG - chr2.4:4795924-4795943 None:intergenic 30.0%
ACCGACACTTGAAAGATTAA+AGG + chr2.4:4796312-4796331 MS.gene85253:intron 35.0%
AGGTTGTTCAATGAGATGAT+CGG + chr2.4:4796750-4796769 MS.gene85253:CDS 35.0%
ATCGTGAATGTCAATGAATC+CGG - chr2.4:4796849-4796868 None:intergenic 35.0%
CAAGTACGTCACAAACTATA+AGG - chr2.4:4797075-4797094 None:intergenic 35.0%
GAAGGATATTAACCTGTATG+TGG + chr2.4:4796282-4796301 MS.gene85253:intron 35.0%
GAGAAAATAGGAATGATCGA+AGG - chr2.4:4796396-4796415 None:intergenic 35.0%
GCAATTTAGACTTGAGAACT+TGG - chr2.4:4795510-4795529 None:intergenic 35.0%
GCCAAGACAATTAAAACATC+AGG - chr2.4:4795547-4795566 None:intergenic 35.0%
GCTTAAACCTTAACCAAACA+GGG - chr2.4:4795629-4795648 None:intergenic 35.0%
TATTACCGGTGTTTATGTGT+GGG + chr2.4:4796420-4796439 MS.gene85253:intron 35.0%
TCTGACACTATATTGTTGCT+GGG + chr2.4:4796654-4796673 MS.gene85253:CDS 35.0%
TGCTTAAACCTTAACCAAAC+AGG - chr2.4:4795630-4795649 None:intergenic 35.0%
TTCTGACACTATATTGTTGC+TGG + chr2.4:4796653-4796672 MS.gene85253:CDS 35.0%
! AGAGATGTTCAATCAAGTTG+TGG + chr2.4:4796704-4796723 MS.gene85253:CDS 35.0%
! ATTAGGATTGAAGGTGTGTT+TGG + chr2.4:4796343-4796362 MS.gene85253:intron 35.0%
! GAATAATGGAGAACATGAAC+TGG + chr2.4:4796887-4796906 MS.gene85253:CDS 35.0%
! GTTCAATCAAGTTGTGGAAA+AGG + chr2.4:4796710-4796729 MS.gene85253:CDS 35.0%
! TCAATCAAGTTGTGGAAAAG+GGG + chr2.4:4796712-4796731 MS.gene85253:CDS 35.0%
! TGACATTCACGATACTGATT+AGG + chr2.4:4796856-4796875 MS.gene85253:CDS 35.0%
!!! GCCTGATGTTTTAATTGTCT+TGG + chr2.4:4795543-4795562 MS.gene85253:CDS 35.0%
AGGAAGCTGGGTATGATAAA+TGG + chr2.4:4796616-4796635 MS.gene85253:CDS 40.0%
ATCGGGGTTTATTTGCAAGT+TGG + chr2.4:4796768-4796787 MS.gene85253:CDS 40.0%
CCAACTCCATTTATCTCTCA+AGG + chr2.4:4795585-4795604 MS.gene85253:CDS 40.0%
CCTTGAGAGATAAATGGAGT+TGG - chr2.4:4795588-4795607 None:intergenic 40.0%
CGAGTTTATCATCAGTGATG+AGG + chr2.4:4796596-4796615 MS.gene85253:CDS 40.0%
GAAAACTCACATGTTTCAGC+TGG - chr2.4:4796977-4796996 None:intergenic 40.0%
GAACAAGATGATTGAGATGG+CGG + chr2.4:4796680-4796699 MS.gene85253:CDS 40.0%
GGTTGTTCAATGAGATGATC+GGG + chr2.4:4796751-4796770 MS.gene85253:CDS 40.0%
GTATTACCGGTGTTTATGTG+TGG + chr2.4:4796419-4796438 MS.gene85253:intron 40.0%
GTTGTTCAATGAGATGATCG+GGG + chr2.4:4796752-4796771 MS.gene85253:CDS 40.0%
TATTAACCTGTATGTGGCAG+TGG + chr2.4:4796288-4796307 MS.gene85253:intron 40.0%
TCATTGAACAACCTCGTATC+TGG - chr2.4:4796744-4796763 None:intergenic 40.0%
TGATACCCACACATAAACAC+CGG - chr2.4:4796428-4796447 None:intergenic 40.0%
ACAGATCCATGAAGGCTTCA+GGG + chr2.4:4796817-4796836 MS.gene85253:CDS 45.0%
ATCATCAGTGATGAGGAAGC+TGG + chr2.4:4796603-4796622 MS.gene85253:CDS 45.0%
GGAGGTTTACAGATCCATGA+AGG + chr2.4:4796809-4796828 MS.gene85253:CDS 45.0%
TACAGATCCATGAAGGCTTC+AGG + chr2.4:4796816-4796835 MS.gene85253:CDS 45.0%
TCATCAGTGATGAGGAAGCT+GGG + chr2.4:4796604-4796623 MS.gene85253:CDS 45.0%
!! GCAAGTTGGTAATACGGAGA+AGG + chr2.4:4796782-4796801 MS.gene85253:CDS 45.0%
!! AAAATTAAAATGAAAAAATA+AGG - chr2.4:4796549-4796568 None:intergenic 5.0%
AGGGGTTGAAACCAGATACG+AGG + chr2.4:4796730-4796749 MS.gene85253:CDS 50.0%
CAAACACCCTGAAGCCTTCA+TGG - chr2.4:4796826-4796845 None:intergenic 50.0%
TAATACGGAGAAGGCGATGG+AGG + chr2.4:4796791-4796810 MS.gene85253:CDS 50.0%
TGGTAATACGGAGAAGGCGA+TGG + chr2.4:4796788-4796807 MS.gene85253:CDS 50.0%
TCGGTACCACTGCCACATAC+AGG - chr2.4:4796297-4796316 None:intergenic 55.0%
! GAAGGCTTCAGGGTGTTTGC+CGG + chr2.4:4796827-4796846 MS.gene85253:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4795433 4797126 4795433 ID=MS.gene85253
chr2.4 mRNA 4795433 4797126 4795433 ID=MS.gene85253.t1;Parent=MS.gene85253
chr2.4 exon 4795433 4795606 4795433 ID=MS.gene85253.t1.exon1;Parent=MS.gene85253.t1
chr2.4 CDS 4795433 4795606 4795433 ID=cds.MS.gene85253.t1;Parent=MS.gene85253.t1
chr2.4 exon 4796591 4796986 4796591 ID=MS.gene85253.t1.exon2;Parent=MS.gene85253.t1
chr2.4 CDS 4796591 4796986 4796591 ID=cds.MS.gene85253.t1;Parent=MS.gene85253.t1
chr2.4 exon 4797106 4797126 4797106 ID=MS.gene85253.t1.exon3;Parent=MS.gene85253.t1
chr2.4 CDS 4797106 4797126 4797106 ID=cds.MS.gene85253.t1;Parent=MS.gene85253.t1
Gene Sequence

>MS.gene85253

ATGGTAATTTCCAAAGAAAGCGTTCAAGTTATTCACGCTCTCAAACTCGCTAAAAATTCCGAAGAAAAACTCAACCAAGTTCTCAAGTCTAAATTGCTTAGGTTATTGAAGCCTGATGTTTTAATTGTCTTGGCTGAATTGCATAGACAAAACCAACTCCATTTATCTCTCAAGGTAAAAATCACAATTAAAACCCTGTTTGGTTAAGGTTTAAGCACTTATTGCATAAGTGATTATCATGTACATGTTATTATGCACTTATTTTATTTGAGCTTATTTACTAATATAATTTGAGAGAGCTTATAAAAATACTTATGATATGCCTATAAGTTGTTTGCAACTTGTTTCTAATAAGCTATCTAGGACGGCTTATGAAAACAAACAACTTATAACTTATATGAAAATTATATGGCTTTATTTTATCTTTTGATAGATAAATATCTTATTGATGAGAAATTATATGACTAACACTTAATCAAGTTGTTTATCCAATTCCAAACAAAGCCAATATCAAACTATTATCTTATAAGCTATAAGCTTTTTTCATAAATTATTCTGGAGAACATGTGAAAATAAGCTGAAAACTACTCACGGACATGTCAAAAACTGTTTTCTCTAGTTTTTTTGGAGCAGTCTAAAAAGTTTCTGTGCTAATAGAGAGACTCAAATAATTCAATTCAAACACCCAAATTACACTAGTGACTAACTATTTTTTGTGCGTCTAAATTCTCCGTTCTTATAGAAAATGATATCCAACTAATTAGAAGTTCTGTTGATAGTTAAGTTGCTAGAAAATTATTTCATCTAGGTATCAAACTCATATACTAATTTAATAATTTGATCGTAGTTGAAGGATATTAACCTGTATGTGGCAGTGGTACCGACACTTGAAAGATTAAAGGTCTTAAGAATTAGGATTGAAGGTGTGTTTGGTGTCACATGACACAACATAAGCAGTCACCTTCGATCATTCCTATTTTCTCAAAGTATTACCGGTGTTTATGTGTGGGTATCATGTCTGTTGTCAATGTATGTGTCAATGCTTTACATGCATTAACAACTTGCTTCTAATCAGTTAGTTACCAGTAGCTAATAATTCTAACTACTCTTTCTCCTTATTTTTTCATTTTAATTTTGCATTTGATAATTGTAACATAGGTTTTCGAGTTTATCATCAGTGATGAGGAAGCTGGGTATGATAAATGGCTGCTGCCATTGTATTCTGACACTATATTGTTGCTGGGAAAGAACAAGATGATTGAGATGGCGGAAGAGATGTTCAATCAAGTTGTGGAAAAGGGGTTGAAACCAGATACGAGGTTGTTCAATGAGATGATCGGGGTTTATTTGCAAGTTGGTAATACGGAGAAGGCGATGGAGGTTTACAGATCCATGAAGGCTTCAGGGTGTTTGCCGGATTCATTGACATTCACGATACTGATTAGGAATCTAATGAATAATGGAGAACATGAACTGGTTGAAACTTTGAAAAAAGAGAGTTTTGACTATGTCAATAAACCTGATAAATTTGTTCAGAAAGTCCAGCTGAAACATGTGAGTTTTCAAATTTTATGGTGTTATGTTGTGCAACTATTGTTTTCTCTTTCTCTGATTTTTTAAGTGACATTTTGGAATTATACCTTATAGTTTGTGACGTACTTGTAAACTTGTAGTGTCTTGTATGGGAAGAGTAA

Protein sequence

>MS.gene85253.t1

MVISKESVQVIHALKLAKNSEEKLNQVLKSKLLRLLKPDVLIVLAELHRQNQLHLSLKVFEFIISDEEAGYDKWLLPLYSDTILLLGKNKMIEMAEEMFNQVVEKGLKPDTRLFNEMIGVYLQVGNTEKAMEVYRSMKASGCLPDSLTFTILIRNLMNNGEHELVETLKKESFDYVNKPDKFVQKVQLKHCLVWEE