AlfalfaGEDB | MsG0180001860.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001860.01

KEGG: -
GO: GO:0016021
PFAM: PF01151

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001860.01.T01	XP_003626313.1	95.082	61	3	0	1	61	157	217	3.23E-32	122

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001860.01.T01	Q2HTN1	95.082	61	3	0	1	61	157	217	1.54e-32	122

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048528.01	MsG0180001860.01	0.810820	9.530201e-51	5.803717e-48
MsG0080048929.01	MsG0180001860.01	0.803933	2.751742e-49	1.400630e-46
MsG0080049004.01	MsG0180001860.01	0.828004	1.143562e-54	1.122444e-51
MsG0080049054.01	MsG0180001860.01	0.807512	4.875186e-50	2.720697e-47
MsG0180000325.01	MsG0180001860.01	0.800190	1.619238e-48	7.492076e-46
MsG0180000638.01	MsG0180001860.01	0.801156	1.028446e-48	4.876822e-46
MsG0180001175.01	MsG0180001860.01	0.803464	3.443316e-49	1.731501e-46
MsG0180001860.01	MsG0180001861.01	0.851472	8.679354e-61	1.758503e-57
MsG0180001860.01	MsG0180002671.01	0.821948	3.077290e-53	2.539881e-50
MsG0180001860.01	MsG0180003453.01	0.820920	5.314660e-53	4.262107e-50
MsG0180001860.01	MsG0180003986.01	0.817215	3.700845e-52	2.678765e-49
MsG0180001860.01	MsG0180005276.01	0.828560	8.399181e-55	8.378347e-52
MsG0180001860.01	MsG0180006042.01	0.821498	3.909997e-53	3.186240e-50
MsG0180001860.01	MsG0180006176.01	0.804594	2.003898e-49	1.037382e-46
MsG0180001860.01	MsG0180006223.01	0.805729	1.159807e-49	6.182512e-47
MsG0180001860.01	MsG0280006825.01	0.809950	1.468331e-50	8.738714e-48
MsG0180001860.01	MsG0280007079.01	0.825130	5.543201e-54	5.008701e-51
MsG0180001860.01	MsG0280007800.01	0.805240	1.468594e-49	7.730146e-47
MsG0180001860.01	MsG0280007852.01	0.812210	4.755189e-51	3.004951e-48
MsG0180001860.01	MsG0280008227.01	0.801059	1.076319e-48	5.091526e-46
MsG0180001860.01	MsG0280008591.01	0.811939	5.447357e-51	3.416903e-48
MsG0180001860.01	MsG0280008935.01	0.800164	1.638506e-48	7.576122e-46
MsG0180001860.01	MsG0280009430.01	0.820575	6.379620e-53	5.066856e-50
MsG0180001860.01	MsG0280009431.01	0.802613	5.161797e-49	2.539827e-46
MsG0180001860.01	MsG0280009751.01	0.801716	7.896038e-49	3.797343e-46
MsG0180001860.01	MsG0280010496.01	0.804837	1.783278e-49	9.290382e-47
MsG0180001860.01	MsG0280011276.01	0.800699	1.275149e-48	5.976642e-46
MsG0180001860.01	MsG0380011632.01	0.802749	4.839120e-49	2.389405e-46
MsG0180001860.01	MsG0380011825.01	0.809198	2.130184e-50	1.242759e-47
MsG0180001860.01	MsG0380011896.01	0.816454	5.484325e-52	3.887612e-49
MsG0180001860.01	MsG0380012876.01	0.803118	4.060179e-49	2.023577e-46
MsG0180001860.01	MsG0380013530.01	0.814592	1.424061e-51	9.595396e-49
MsG0180001860.01	MsG0380015081.01	0.801060	1.075905e-48	5.089701e-46
MsG0180001860.01	MsG0380015103.01	0.818518	1.879545e-52	1.410070e-49
MsG0180001860.01	MsG0380015356.01	0.804224	2.393459e-49	1.227256e-46
MsG0180001860.01	MsG0380016478.01	0.801819	7.522883e-49	3.627448e-46
MsG0180001860.01	MsG0380016549.01	0.828872	7.060165e-55	7.105858e-52
MsG0180001860.01	MsG0380017100.01	0.814649	1.383536e-51	9.336529e-49
MsG0180001860.01	MsG0480018108.01	0.813519	2.456329e-51	1.607676e-48
MsG0180001860.01	MsG0480018592.01	0.825091	5.662129e-54	5.110522e-51
MsG0180001860.01	MsG0480018676.01	0.808878	2.494218e-50	1.442616e-47
MsG0180001860.01	MsG0480021307.01	0.802283	6.038305e-49	2.946311e-46
MsG0180001860.01	MsG0480021317.01	0.800396	1.469777e-48	6.836447e-46
MsG0180001860.01	MsG0480021521.01	0.806855	6.717003e-50	3.685154e-47
MsG0180001860.01	MsG0480022366.01	0.802349	5.851929e-49	2.860189e-46
MsG0180001860.01	MsG0480022417.01	0.804992	1.654820e-49	8.655250e-47
MsG0180001860.01	MsG0480022626.01	0.810356	1.200346e-50	7.219902e-48
MsG0180001860.01	MsG0480023148.01	0.816083	6.638766e-52	4.658909e-49
MsG0180001860.01	MsG0480023351.01	0.823350	1.452574e-53	1.247491e-50
MsG0180001860.01	MsG0480023483.01	0.803680	3.105073e-49	1.570076e-46
MsG0180001860.01	MsG0480023502.01	0.828440	8.982083e-55	8.928701e-52
MsG0180001860.01	MsG0480023704.01	0.802153	6.422862e-49	3.123462e-46
MsG0180001860.01	MsG0480023818.01	0.806777	6.976818e-50	3.819820e-47
MsG0180001860.01	MsG0580024128.01	0.813329	2.704251e-51	1.760767e-48
MsG0180001860.01	MsG0580024327.01	0.819026	1.441812e-52	1.096999e-49
MsG0180001860.01	MsG0580024385.01	0.810283	1.245139e-50	7.474496e-48
MsG0180001860.01	MsG0580024836.01	0.820166	7.919922e-53	6.219827e-50
MsG0180001860.01	MsG0580024950.01	0.828168	1.044418e-54	1.030003e-51
MsG0180001860.01	MsG0580026211.01	0.821307	4.329699e-53	3.509587e-50
MsG0180001860.01	MsG0580028461.01	0.812653	3.805058e-51	2.433005e-48
MsG0180001860.01	MsG0580029816.01	0.800697	1.276158e-48	5.981138e-46
MsG0180001860.01	MsG0680030754.01	0.802552	5.315069e-49	2.611097e-46
MsG0180001860.01	MsG0680030957.01	0.803296	3.730999e-49	1.868001e-46
MsG0180001860.01	MsG0680032238.01	0.802788	4.750677e-49	2.348070e-46
MsG0180001860.01	MsG0680032520.01	0.811789	5.872585e-51	3.668979e-48
MsG0180001860.01	MsG0680035619.01	0.810290	1.240384e-50	7.447517e-48
MsG0180001860.01	MsG0780037184.01	0.815309	9.876171e-52	6.784975e-49
MsG0180001860.01	MsG0780037436.01	0.821716	3.482308e-53	2.855334e-50
MsG0180001860.01	MsG0780037655.01	0.806530	7.865261e-50	4.279112e-47
MsG0180001860.01	MsG0780039746.01	0.821927	3.112248e-53	2.567263e-50
MsG0180001860.01	MsG0780040159.01	0.803191	3.922140e-49	1.958516e-46
MsG0180001860.01	MsG0780040331.01	0.802298	5.996161e-49	2.926860e-46
MsG0180001860.01	MsG0780040412.01	0.815742	7.908313e-52	5.498492e-49
MsG0180001860.01	MsG0780040644.01	0.810944	8.959447e-51	5.474612e-48
MsG0180001860.01	MsG0780040884.01	0.812770	3.587518e-51	2.300930e-48
MsG0180001860.01	MsG0780041706.01	0.806205	9.211629e-50	4.970433e-47
MsG0180001860.01	MsG0780041778.01	0.804732	1.875944e-49	9.746824e-47
MsG0180001860.01	MsG0880041931.01	0.810517	1.108510e-50	6.695785e-48
MsG0180001860.01	MsG0880042889.01	0.801458	8.917317e-49	4.260759e-46
MsG0180001860.01	MsG0880043497.01	0.802146	6.441477e-49	3.131996e-46
MsG0180001860.01	MsG0880043913.01	0.807506	4.889385e-50	2.728252e-47
MsG0180001860.01	MsG0880044228.01	0.813187	2.906271e-51	1.884858e-48
MsG0180001860.01	MsG0880044595.01	0.801593	8.369323e-49	4.012383e-46
MsG0180001860.01	MsG0880045697.01	0.824745	6.834360e-54	6.108050e-51
MsG0180001860.01	MsG0880046104.01	0.801681	8.026410e-49	3.856612e-46
MsG0180001860.01	MsG0880046252.01	0.807100	5.960635e-50	3.290777e-47
MsG0180001860.01	MsG0880046791.01	0.806032	1.001461e-49	5.379727e-47
MsG0180001860.01	MsG0880046956.01	0.804325	2.280439e-49	1.172385e-46
MsG0180001860.01	MsG0880047234.01	0.828800	7.348503e-55	7.381146e-52
MsG0180001860.01	MsG0880047567.01	0.815164	1.063713e-51	7.278418e-49
MsG0180001860.01	MsG0880047616.01	0.807678	4.495023e-50	2.519285e-47
MsG0180001860.01	MsG0880047670.01	0.812763	3.598681e-51	2.307734e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001860.01.T01	MTR_7g113780	95.082	61	3	0	1	61	157	217	3.91e-36	122

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001860.01.T01	AT3G06470	73.770	61	16	0	1	61	164	224	3.77e-28	102
MsG0180001860.01.T01	AT3G06460	53.968	63	29	0	1	63	161	223	2.57e-21	84.7

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTACTGGTGGTGGATGCTC+TGG	0.317198	1:-28346791	MsG0180001860.01.T01:CDS
TCCCTATCATGTGTTATCTA+TGG	0.395738	1:-28347011	None:intergenic
GAGTTGGTAAGAAGTGTAAT+CGG	0.404191	1:+28346964	None:intergenic
CGTGTTCAACTTCGCCGTCT+CGG	0.456591	1:-28346842	MsG0180001860.01.T01:CDS
AGAGCATCCACCACCAGTAC+TGG	0.464465	1:+28346793	None:intergenic
TACTACTTCCTCACCACTGT+TGG	0.476064	1:-28346916	MsG0180001860.01.T01:CDS
CTCATGCTTTATTATCACTT+CGG	0.480515	1:-28346818	MsG0180001860.01.T01:CDS
AGTTGGTAAGAAGTGTAATC+GGG	0.497030	1:+28346965	None:intergenic
TATCACTTCGGATCCAGTAC+TGG	0.500674	1:-28346806	MsG0180001860.01.T01:CDS
TGAACACGAACTGAACAATC+TGG	0.522127	1:+28346857	None:intergenic
GTACTGGTGGTGGATGCTCT+GGG	0.537624	1:-28346790	MsG0180001860.01.T01:CDS
TTCGGATCCAGTACTGGTGG+TGG	0.542647	1:-28346800	MsG0180001860.01.T01:CDS
TCGGTAACGATGCGTTTCCA+CGG	0.575404	1:+28346883	None:intergenic
CGATGCGTTTCCACGGCGGT+CGG	0.592491	1:+28346890	None:intergenic
CACTTCGGATCCAGTACTGG+TGG	0.625047	1:-28346803	MsG0180001860.01.T01:CDS
GTCGGATTCCAACAGTGGTG+AGG	0.628146	1:+28346908	None:intergenic
CGGCGGTCGGATTCCAACAG+TGG	0.641832	1:+28346903	None:intergenic
GAACTGAACAATCTGGCAGT+CGG	0.662736	1:+28346864	None:intergenic
GATGACGTGGACAGATGAGT+TGG	0.664978	1:+28346948	None:intergenic
AATAAAGCATGAGACCGAGA+CGG	0.697098	1:+28346828	None:intergenic
AGTAAGAGTACATGATGACG+TGG	0.708386	1:+28346935	None:intergenic
CTGTTGGAATCCGACCGCCG+TGG	0.731632	1:-28346900	MsG0180001860.01.T01:CDS
GTAACGATGCGTTTCCACGG+CGG	0.756696	1:+28346886	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTCATGCTTTATTATCACTT+CGG	-	Chr1:28346971-28346990	MsG0180001860.01.T01:CDS	30.0%
!!	AGTTGGTAAGAAGTGTAATC+GGG	+	Chr1:28346827-28346846	None:intergenic	35.0%
!!	GAGTTGGTAAGAAGTGTAAT+CGG	+	Chr1:28346828-28346847	None:intergenic	35.0%
	AATAAAGCATGAGACCGAGA+CGG	+	Chr1:28346964-28346983	None:intergenic	40.0%
	TGAACACGAACTGAACAATC+TGG	+	Chr1:28346935-28346954	None:intergenic	40.0%
!	AGTAAGAGTACATGATGACG+TGG	+	Chr1:28346857-28346876	None:intergenic	40.0%
	GAACTGAACAATCTGGCAGT+CGG	+	Chr1:28346928-28346947	None:intergenic	45.0%
	TACTACTTCCTCACCACTGT+TGG	-	Chr1:28346873-28346892	MsG0180001860.01.T01:CDS	45.0%
	TATCACTTCGGATCCAGTAC+TGG	-	Chr1:28346983-28347002	MsG0180001860.01.T01:CDS	45.0%
	GATGACGTGGACAGATGAGT+TGG	+	Chr1:28346844-28346863	None:intergenic	50.0%
	TCGGTAACGATGCGTTTCCA+CGG	+	Chr1:28346909-28346928	None:intergenic	50.0%
	CGTGTTCAACTTCGCCGTCT+CGG	-	Chr1:28346947-28346966	MsG0180001860.01.T01:CDS	55.0%
	GTAACGATGCGTTTCCACGG+CGG	+	Chr1:28346906-28346925	None:intergenic	55.0%
	GTCGGATTCCAACAGTGGTG+AGG	+	Chr1:28346884-28346903	None:intergenic	55.0%
!	AGAGCATCCACCACCAGTAC+TGG	+	Chr1:28346999-28347018	None:intergenic	55.0%
!	CACTTCGGATCCAGTACTGG+TGG	-	Chr1:28346986-28347005	MsG0180001860.01.T01:CDS	55.0%
!!	AGTACTGGTGGTGGATGCTC+TGG	-	Chr1:28346998-28347017	MsG0180001860.01.T01:CDS	55.0%
!!	GTACTGGTGGTGGATGCTCT+GGG	-	Chr1:28346999-28347018	MsG0180001860.01.T01:CDS	55.0%
!!	TTCGGATCCAGTACTGGTGG+TGG	-	Chr1:28346989-28347008	MsG0180001860.01.T01:CDS	55.0%
	CGATGCGTTTCCACGGCGGT+CGG	+	Chr1:28346902-28346921	None:intergenic	65.0%
	CGGCGGTCGGATTCCAACAG+TGG	+	Chr1:28346889-28346908	None:intergenic	65.0%
	CTGTTGGAATCCGACCGCCG+TGG	-	Chr1:28346889-28346908	MsG0180001860.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	28346786	28347025	28346786	ID=MsG0180001860.01;Name=MsG0180001860.01
Chr1	mRNA	28346786	28347025	28346786	ID=MsG0180001860.01.T01;Parent=MsG0180001860.01;Name=MsG0180001860.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|79
Chr1	exon	28346786	28347025	28346786	ID=MsG0180001860.01.T01:exon:18845;Parent=MsG0180001860.01.T01
Chr1	CDS	28346786	28347025	28346786	ID=MsG0180001860.01.T01:cds;Parent=MsG0180001860.01.T01

Gene Sequence	>MsG0180001860.01.T01 ATGTGTTATCTATGGCTTAATTCTTCTCAATCACTATTCCCGATTACACTTCTTACCAACTCATCTGTCCACGTCATCATGTACTCTTACTACTTCCTCACCACTGTTGGAATCCGACCGCCGTGGAAACGCATCGTTACCGACTGCCAGATTGTTCAGTTCGTGTTCAACTTCGCCGTCTCGGTCTCATGCTTTATTATCACTTCGGATCCAGTACTGGTGGTGGATGCTCTGGGATGA
Protein sequence	>MsG0180001860.01.T01 MCYLWLNSSQSLFPITLLTNSSVHVIMYSYYFLTTVGIRPPWKRIVTDCQIVQFVFNFAVSVSCFIITSDPVLVVDALG*