AlfalfaGEDB | MsG0680032520.01

M. sativa cultivar ZhongmuNo.1 / MsG0680032520.01

KEGG: -
GO: GO:0005524
PFAM: PF01883 PF10609

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032520.01.T01	XP_003620543.2	87.936	373	14	3	1	342	1	373	0	645

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032520.01.T01	Q6STH5	78.644	295	34	2	77	342	78	372	8.71E-162	466

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032520.01.T01	G7KNK7	87.936	373	14	3	1	342	1	373	0.0	645

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047910.01	MsG0680032520.01	0.831112	2.007373e-55	2.157304e-52
MsG0080047945.01	MsG0680032520.01	0.821958	3.060733e-53	2.526890e-50
MsG0080047955.01	MsG0680032520.01	0.847471	1.132835e-59	2.015737e-56
MsG0080047956.01	MsG0680032520.01	0.819446	1.156544e-52	8.902016e-50
MsG0080047989.01	MsG0680032520.01	0.816010	6.893967e-52	4.828139e-49
MsG0080048068.01	MsG0680032520.01	0.881069	3.259059e-70	1.881915e-66
MsG0080048198.01	MsG0680032520.01	0.813711	2.228707e-51	1.466338e-48
MsG0080048505.01	MsG0680032520.01	0.805531	1.276375e-49	6.768622e-47
MsG0080048528.01	MsG0680032520.01	0.898731	3.980770e-77	4.766516e-73
MsG0080048727.01	MsG0680032520.01	0.816395	5.653125e-52	4.001035e-49

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032520.01.T01	MTR_6g086600	87.936	373	14	3	1	342	1	373	0.0	645
MsG0680032520.01.T01	MTR_6g086600	87.393	349	15	2	23	342	16	364	0.0	602
MsG0680032520.01.T01	MTR_1g070360	40.541	148	78	3	184	326	54	196	3.33e-33	125
MsG0680032520.01.T01	MTR_1g016110	35.795	176	92	4	153	323	28	187	4.04e-18	84.3
MsG0680032520.01.T01	MTR_1g019620	35.795	176	92	4	153	323	75	234	1.44e-17	83.6
MsG0680032520.01.T01	MTR_1g016110	35.795	176	92	4	153	323	75	234	1.51e-17	83.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032520.01.T01	AT3G24430	78.644	295	34	2	77	342	78	372	8.89e-163	466
MsG0680032520.01.T01	AT4G19540	43.448	145	78	3	184	326	75	217	2.49e-32	124
MsG0680032520.01.T01	AT5G50960	35.754	179	103	5	159	332	72	243	4.83e-23	99.0

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Find 115 sgRNAs with CRISPR-Local

Find 380 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGACCAAAGTTCATTA+TGG	0.349495	6:-43627195	MsG0680032520.01.T01:CDS
AACCTGAAGAAATTCGGCAA+TGG	0.556115	6:-43622487	MsG0680032520.01.T01:three_prime_UTR
AACTTCTCACTACTACCGAG+TGG	0.751912	6:-43624828	MsG0680032520.01.T01:intron
AAGAAGTCCATAATGAACTT+TGG	0.449601	6:+43627188	None:intergenic
AATTATGCCGTTTCAATAAC+AGG	0.287392	6:-43622462	MsG0680032520.01.T01:three_prime_UTR
ACAATATCCGTTCCAAAATC+TGG	0.310740	6:+43625934	None:intergenic
ACAATTACAGCAGCAGTTAA+TGG	0.320244	6:+43623927	None:intergenic
ACATTGAACCTGAAGAAATT+CGG	0.416513	6:-43622493	MsG0680032520.01.T01:three_prime_UTR
ACATTGACATTCTTGACCCA+AGG	0.579491	6:+43625458	None:intergenic
ACCTGAAGAAATTCGGCAAT+GGG	0.451537	6:-43622486	MsG0680032520.01.T01:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAGTCCATAATGAACTT+TGG	+	Chr6:43621866-43621885	None:intergenic	30.0%
AAGTTGAACCTAAAGCAAAA+TGG	+	Chr6:43627084-43627103	None:intergenic	30.0%
ACACTTTCGGACAAATTATA+TGG	+	Chr6:43626910-43626929	None:intergenic	30.0%
ACATTGAACCTGAAGAAATT+CGG	-	Chr6:43626558-43626577	MsG0680032520.01.T01:intron	30.0%
AGATATGCTATGCATAGAAT+AGG	-	Chr6:43624274-43624293	MsG0680032520.01.T01:intron	30.0%
AGTAAACCTTGCTTATACTT+TGG	-	Chr6:43623904-43623923	MsG0680032520.01.T01:CDS	30.0%
ATGAGACGAAATGTAAATCA+TGG	+	Chr6:43623819-43623838	None:intergenic	30.0%
CATAATTCCAACTGAATACA+TGG	-	Chr6:43624119-43624138	MsG0680032520.01.T01:intron	30.0%
CATATCAAGCATAACCTATT+AGG	+	Chr6:43624527-43624546	None:intergenic	30.0%
CATGTATTCAGTTGGAATTA+TGG	+	Chr6:43624121-43624140	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	43621411	43627662	43621411	ID=MsG0680032520.01;Name=MsG0680032520.01
Chr6	mRNA	43621411	43627662	43621411	ID=MsG0680032520.01.T01;Parent=MsG0680032520.01;Name=MsG0680032520.01.T01;_AED=0.49;_eAED=0.49;_QI=38\|0.85\|0.86\|1\|0.78\|0.73\|15\|696\|370
Chr6	exon	43627555	43627662	43627555	ID=MsG0680032520.01.T01:exon:11661;Parent=MsG0680032520.01.T01
Chr6	exon	43627131	43627262	43627131	ID=MsG0680032520.01.T01:exon:11662;Parent=MsG0680032520.01.T01
Chr6	exon	43625885	43626027	43625885	ID=MsG0680032520.01.T01:exon:11663;Parent=MsG0680032520.01.T01
Chr6	exon	43625745	43625795	43625745	ID=MsG0680032520.01.T01:exon:11664;Parent=MsG0680032520.01.T01
Chr6	exon	43625361	43625516	43625361	ID=MsG0680032520.01.T01:exon:11665;Parent=MsG0680032520.01.T01
Chr6	exon	43625053	43625130	43625053	ID=MsG0680032520.01.T01:exon:11666;Parent=MsG0680032520.01.T01
Chr6	exon	43624829	43624971	43624829	ID=MsG0680032520.01.T01:exon:11667;Parent=MsG0680032520.01.T01
Chr6	exon	43624429	43624498	43624429	ID=MsG0680032520.01.T01:exon:11668;Parent=MsG0680032520.01.T01
Chr6	exon	43623860	43623955	43623860	ID=MsG0680032520.01.T01:exon:11669;Parent=MsG0680032520.01.T01
Chr6	exon	43623662	43623730	43623662	ID=MsG0680032520.01.T01:exon:11670;Parent=MsG0680032520.01.T01
Chr6	exon	43623468	43623519	43623468	ID=MsG0680032520.01.T01:exon:11671;Parent=MsG0680032520.01.T01
Chr6	exon	43623222	43623343	43623222	ID=MsG0680032520.01.T01:exon:11672;Parent=MsG0680032520.01.T01
Chr6	exon	43622639	43622758	43622639	ID=MsG0680032520.01.T01:exon:11673;Parent=MsG0680032520.01.T01
Chr6	exon	43622443	43622562	43622443	ID=MsG0680032520.01.T01:exon:11674;Parent=MsG0680032520.01.T01
Chr6	exon	43621411	43621797	43621411	ID=MsG0680032520.01.T01:exon:11675;Parent=MsG0680032520.01.T01
Chr6	five_prime_UTR	43627625	43627662	43627625	ID=MsG0680032520.01.T01:five_prime_utr;Parent=MsG0680032520.01.T01
Chr6	CDS	43627555	43627624	43627555	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43627131	43627262	43627131	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43625885	43626027	43625885	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43625745	43625795	43625745	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43625361	43625516	43625361	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43625053	43625130	43625053	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43624829	43624971	43624829	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43624429	43624498	43624429	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43623860	43623955	43623860	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43623662	43623730	43623662	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43623468	43623519	43623468	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	CDS	43623291	43623343	43623291	ID=MsG0680032520.01.T01:cds;Parent=MsG0680032520.01.T01
Chr6	three_prime_UTR	43623222	43623290	43623222	ID=MsG0680032520.01.T01:three_prime_utr;Parent=MsG0680032520.01.T01
Chr6	three_prime_UTR	43622639	43622758	43622639	ID=MsG0680032520.01.T01:three_prime_utr;Parent=MsG0680032520.01.T01
Chr6	three_prime_UTR	43622443	43622562	43622443	ID=MsG0680032520.01.T01:three_prime_utr;Parent=MsG0680032520.01.T01
Chr6	three_prime_UTR	43621411	43621797	43621411	ID=MsG0680032520.01.T01:three_prime_utr;Parent=MsG0680032520.01.T01

Gene Sequence

>MsG0680032520.01.T01

ATGCAAACTGTTCAAGCTTCATCACACTTTTCTATCCATTCCTCAAAACCACCACACTCATCAACATGCAGTTTGGTTACTTCTTCTGTGAATGTAAAGTGTTCAGGTTTTTCTCAAAGGGACCAAAGTTCATTATGGACTTCTTATAATAAGAGGGTTATCTTGAAAAGCTCTTTTTCTGCTAGAGCTGCTTCTGTTGAAGTTGGAAGTTCCTCGGTATCAACTGGAACAGCCGAAGATGATGTATTGAAAGCCTTGTCTCAGATCATTGATCCAGATTTTGGAACGGATATTGTTACTTGTGGATTTGTGAAAGATTTGCAGATCGATAAAGCTCTAGGAGAGGTGTCGTTTCGGTTAGAGCTCACTACACCGGCATGTCCGATCAAGGACGTGTTTGAGAAGCAGGCAAATGAGGTGGTGGCAGTGCTCCCTTGGGTCAAGAATGTCAATGTTACGATGTCTGCGCAGCCAGCAAAACCGTTATTCGCAGAGCAACTTCCAGCAGGTTTACAGACGATTTCAAATATTATCGCTGTATCCAGTTGCAAGAGTTGGTATATTGATGCTGATATTTATGGTCCAAGCTTACCAACTATGGTCTCTCCGGAAAATCGAATATTAGAAATGAATCCAGAAAAGAAGACCATAATTCCAACTGAATACATGGGAGTCAAGTTGGTTTCTTTTGGATTTGCTGGACAAGGACGTGCAATTATGCGCGGCCCTATGGTTTCTGGAGTCACTAACCAACTTCTCACTACTACCGAGTGGGGCGAGCTTGATTACCTTGTTATCGACATGCCTCCGGGAACAGGAGACATTCAACTCACTTTATGCCAGATAGTCCCATTAACTGCTGCTGTAATTGTTACCACTCCTCAAAAACTATCGTTCATCGATGTTGCAAAAGGAGTTCGGATGTTTTCAAAGCTTAAGGTACCTTGTGTAGCTGTTGTCGAGAACATGTGTCATTTTGATGCCGATGGAAAAGATACTATCCATTTGGTTGTTCAGCAGTTTGGATACCGCATCTATTTGATCTCCCTATTAGCCGACTCTATCCGTTCTGAGATAGTGGAATGCCTGAAGTGGTGGCTGATCCTCAAGTGA

Protein sequence

>MsG0680032520.01.T01

MQTVQASSHFSIHSSKPPHSSTCSLVTSSVNVKCSGFSQRDQSSLWTSYNKRVILKSSFSARAASVEVGSSSVSTGTAEDDVLKALSQIIDPDFGTDIVTCGFVKDLQIDKALGEVSFRLELTTPACPIKDVFEKQANEVVAVLPWVKNVNVTMSAQPAKPLFAEQLPAGLQTISNIIAVSSCKSWYIDADIYGPSLPTMVSPENRILEMNPEKKTIIPTEYMGVKLVSFGFAGQGRAIMRGPMVSGVTNQLLTTTEWGELDYLVIDMPPGTGDIQLTLCQIVPLTAAVIVTTPQKLSFIDVAKGVRMFSKLKVPCVAVVENMCHFDADGKDTIHLVVQQFGYRIYLISLLADSIRSEIVECLKWWLILK*