AlfalfaGEDB | MsG0180001861.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001861.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048059.01	MsG0180001861.01	0.811593	6.477838e-51	4.026103e-48
MsG0080048917.01	MsG0180001861.01	0.804892	1.736827e-49	9.061706e-47
MsG0080048929.01	MsG0180001861.01	0.806165	9.392553e-50	5.062833e-47
MsG0080049004.01	MsG0180001861.01	0.830276	3.216424e-55	3.372857e-52
MsG0080049024.01	MsG0180001861.01	0.830500	2.836368e-55	2.993522e-52
MsG0080049053.01	MsG0180001861.01	0.827184	1.799654e-54	1.724628e-51
MsG0080049062.01	MsG0180001861.01	0.814148	1.785502e-51	1.188673e-48
MsG0180000193.01	MsG0180001861.01	0.828139	1.061261e-54	1.045733e-51
MsG0180000194.01	MsG0180001861.01	0.800204	1.608138e-48	7.443505e-46
MsG0180000325.01	MsG0180001861.01	0.827541	1.477539e-54	1.430665e-51
MsG0180000352.01	MsG0180001861.01	0.807085	6.004719e-50	3.313866e-47
MsG0180000467.01	MsG0180001861.01	0.833847	4.215940e-56	4.915529e-53
MsG0180001113.01	MsG0180001861.01	0.807204	5.666138e-50	3.136825e-47
MsG0180001827.01	MsG0180001861.01	0.824072	9.840341e-54	8.625671e-51
MsG0180001860.01	MsG0180001861.01	0.851472	8.679354e-61	1.758503e-57
MsG0180001861.01	MsG0180002671.01	0.824255	8.912197e-54	7.852382e-51
MsG0180001861.01	MsG0180002809.01	0.812903	3.354773e-51	2.159295e-48
MsG0180001861.01	MsG0180003114.01	0.818868	1.566047e-52	1.186280e-49
MsG0180001861.01	MsG0180003296.01	0.823861	1.102811e-53	9.610267e-51
MsG0180001861.01	MsG0180003880.01	0.807241	5.564281e-50	3.083457e-47
MsG0180001861.01	MsG0180003980.01	0.843301	1.524462e-58	2.375032e-55
MsG0180001861.01	MsG0180003985.01	0.811788	5.874369e-51	3.670023e-48
MsG0180001861.01	MsG0180003986.01	0.847048	1.479841e-59	2.597605e-56
MsG0180001861.01	MsG0180004292.01	0.808003	3.834573e-50	2.167571e-47
MsG0180001861.01	MsG0180004553.01	0.802348	5.854496e-49	2.861357e-46
MsG0180001861.01	MsG0180004580.01	0.800237	1.583653e-48	7.336364e-46
MsG0180001861.01	MsG0180004634.01	0.808311	3.296026e-50	1.878349e-47
MsG0180001861.01	MsG0180004747.01	0.833236	5.989660e-56	6.856126e-53
MsG0180001861.01	MsG0180005291.01	0.809107	2.227734e-50	1.296503e-47
MsG0180001861.01	MsG0180006142.01	0.824549	7.599473e-54	6.753250e-51
MsG0180001861.01	MsG0180006159.01	0.820267	7.505905e-53	5.910982e-50
MsG0180001861.01	MsG0180006177.01	0.805876	1.080334e-49	5.780228e-47
MsG0180001861.01	MsG0280006381.01	0.806315	8.730505e-50	4.723815e-47
MsG0180001861.01	MsG0280006452.01	0.812754	3.615890e-51	2.318201e-48
MsG0180001861.01	MsG0280006607.01	0.801226	9.952001e-49	4.727338e-46
MsG0180001861.01	MsG0280006825.01	0.804027	2.630345e-49	1.342009e-46
MsG0180001861.01	MsG0280006973.01	0.812998	3.197570e-51	2.063345e-48
MsG0180001861.01	MsG0280007079.01	0.837190	6.014671e-57	7.753675e-54
MsG0180001861.01	MsG0280007136.01	0.818530	1.868212e-52	1.402024e-49
MsG0180001861.01	MsG0280007247.01	0.847243	1.308247e-59	2.310918e-56
MsG0180001861.01	MsG0280007288.01	0.807533	4.824438e-50	2.693783e-47
MsG0180001861.01	MsG0280007649.01	0.825746	3.961195e-54	3.642598e-51
MsG0180001861.01	MsG0280007650.01	0.829063	6.346995e-55	6.424395e-52
MsG0180001861.01	MsG0280007702.01	0.810051	1.396750e-50	8.334568e-48
MsG0180001861.01	MsG0280008430.01	0.834457	2.965042e-56	3.520891e-53
MsG0180001861.01	MsG0280008935.01	0.852065	5.894188e-61	1.217077e-57
MsG0180001861.01	MsG0280009579.01	0.803268	3.781066e-49	1.891755e-46
MsG0180001861.01	MsG0280009776.01	0.808447	3.083008e-50	1.763301e-47
MsG0180001861.01	MsG0280010149.01	0.828753	7.545376e-55	7.568025e-52
MsG0180001861.01	MsG0280010281.01	0.811999	5.286265e-51	3.321339e-48
MsG0180001861.01	MsG0280010356.01	0.834651	2.649578e-56	3.164300e-53
MsG0180001861.01	MsG0280010704.01	0.836787	7.625169e-57	9.711381e-54
MsG0180001861.01	MsG0280011276.01	0.838391	2.957233e-57	3.955180e-54
MsG0180001861.01	MsG0380011632.01	0.803150	3.999268e-49	1.994917e-46
MsG0180001861.01	MsG0380011896.01	0.844707	6.398964e-59	1.041832e-55
MsG0180001861.01	MsG0380012625.01	0.818307	2.098517e-52	1.565158e-49
MsG0180001861.01	MsG0380013506.01	0.828140	1.060890e-54	1.045393e-51
MsG0180001861.01	MsG0380014266.01	0.837681	4.503316e-57	5.894384e-54
MsG0180001861.01	MsG0380014795.01	0.815451	9.185249e-52	6.335104e-49
MsG0180001861.01	MsG0380015077.01	0.805618	1.223909e-49	6.504974e-47
MsG0180001861.01	MsG0380015081.01	0.841501	4.574227e-58	6.734507e-55
MsG0180001861.01	MsG0380015103.01	0.826714	2.331165e-54	2.203411e-51
MsG0180001861.01	MsG0380015111.01	0.820028	8.516642e-53	6.663085e-50
MsG0180001861.01	MsG0380015439.01	0.808389	3.172022e-50	1.811444e-47
MsG0180001861.01	MsG0380015719.01	0.809690	1.670215e-50	9.872516e-48
MsG0180001861.01	MsG0380015752.01	0.831050	2.079753e-55	2.231059e-52
MsG0180001861.01	MsG0380015795.01	0.829130	6.113985e-55	6.201268e-52
MsG0180001861.01	MsG0380015900.01	0.804969	1.673505e-49	8.747926e-47
MsG0180001861.01	MsG0380015971.01	0.819016	1.449310e-52	1.102428e-49
MsG0180001861.01	MsG0380016166.01	0.824276	8.812142e-54	7.768796e-51
MsG0180001861.01	MsG0380016229.01	0.815643	8.322643e-52	5.770662e-49
MsG0180001861.01	MsG0380016241.01	0.814922	1.203406e-51	8.180448e-49
MsG0180001861.01	MsG0380016478.01	0.803845	2.870079e-49	1.457522e-46
MsG0180001861.01	MsG0380016530.01	0.811603	6.447276e-51	4.008054e-48
MsG0180001861.01	MsG0380016699.01	0.829483	5.019009e-55	5.143471e-52
MsG0180001861.01	MsG0380016790.01	0.812499	4.111594e-51	2.618497e-48
MsG0180001861.01	MsG0380016960.01	0.815141	1.076214e-51	7.359374e-49
MsG0180001861.01	MsG0380017018.01	0.800886	1.167583e-48	5.499082e-46
MsG0180001861.01	MsG0380017309.01	0.804256	2.357437e-49	1.209766e-46
MsG0180001861.01	MsG0380017406.01	0.801531	8.616456e-49	4.124632e-46
MsG0180001861.01	MsG0380017895.01	0.823269	1.517115e-53	1.300016e-50
MsG0180001861.01	MsG0380017962.01	0.801256	9.810760e-49	4.663851e-46
MsG0180001861.01	MsG0380017966.01	0.810762	9.809070e-51	5.964354e-48
MsG0180001861.01	MsG0380018066.01	0.824759	6.782240e-54	6.063773e-51
MsG0180001861.01	MsG0480018108.01	0.818018	2.439723e-52	1.805182e-49
MsG0180001861.01	MsG0480018190.01	0.816897	4.363245e-52	3.130783e-49
MsG0180001861.01	MsG0480018329.01	0.816378	5.704326e-52	4.035391e-49
MsG0180001861.01	MsG0480021039.01	0.801604	8.326811e-49	3.993016e-46
MsG0180001861.01	MsG0480021143.01	0.853463	2.351100e-61	5.084368e-58
MsG0180001861.01	MsG0480021205.01	0.816256	6.074291e-52	4.282976e-49
MsG0180001861.01	MsG0480021307.01	0.803556	3.295235e-49	1.661058e-46
MsG0180001861.01	MsG0480021312.01	0.817028	4.077917e-52	2.936672e-49
MsG0180001861.01	MsG0480021497.01	0.815548	8.736951e-52	6.041909e-49
MsG0180001861.01	MsG0480021521.01	0.816522	5.296903e-52	3.761864e-49
MsG0180001861.01	MsG0480022088.01	0.828862	7.099514e-55	7.143474e-52
MsG0180001861.01	MsG0480022297.01	0.816138	6.453869e-52	4.535788e-49
MsG0180001861.01	MsG0480022366.01	0.835835	1.331448e-56	1.647629e-53
MsG0180001861.01	MsG0480022411.01	0.816526	5.285471e-52	3.754117e-49
MsG0180001861.01	MsG0480022466.01	0.807549	4.787457e-50	2.674201e-47
MsG0180001861.01	MsG0480022584.01	0.809450	1.881039e-50	1.104740e-47
MsG0180001861.01	MsG0480022626.01	0.830981	2.161391e-55	2.314017e-52
MsG0180001861.01	MsG0480022687.01	0.838795	2.325520e-57	3.148875e-54
MsG0180001861.01	MsG0480022837.01	0.821950	3.073611e-53	2.537004e-50
MsG0180001861.01	MsG0480023148.01	0.815105	1.096177e-51	7.488531e-49
MsG0180001861.01	MsG0480023217.01	0.830576	2.716528e-55	2.873564e-52
MsG0180001861.01	MsG0480023469.01	0.806375	8.482193e-50	4.596441e-47
MsG0180001861.01	MsG0480023482.01	0.810855	9.365768e-51	5.709063e-48
MsG0180001861.01	MsG0480023483.01	0.800382	1.479258e-48	6.878361e-46
MsG0180001861.01	MsG0480023531.01	0.803115	4.067115e-49	2.026849e-46
MsG0180001861.01	MsG0480023558.01	0.807201	5.675015e-50	3.141474e-47
MsG0180001861.01	MsG0480023704.01	0.824503	7.791236e-54	6.913999e-51
MsG0180001861.01	MsG0480023705.01	0.811703	6.131953e-51	3.822217e-48
MsG0180001861.01	MsG0480023717.01	0.809476	1.857183e-50	1.091483e-47
MsG0180001861.01	MsG0480023818.01	0.835900	1.281502e-56	1.588958e-53
MsG0180001861.01	MsG0580024128.01	0.849273	3.593714e-60	6.777123e-57
MsG0180001861.01	MsG0580024140.01	0.819776	9.721465e-53	7.552299e-50
MsG0180001861.01	MsG0580024571.01	0.817749	2.805915e-52	2.061027e-49
MsG0180001861.01	MsG0580024678.01	0.808808	2.581602e-50	1.490401e-47
MsG0180001861.01	MsG0580024875.01	0.808093	3.668359e-50	2.078568e-47
MsG0180001861.01	MsG0580024950.01	0.823034	1.721461e-53	1.465499e-50
MsG0180001861.01	MsG0580025105.01	0.805265	1.450794e-49	7.641215e-47
MsG0180001861.01	MsG0580025259.01	0.825262	5.158805e-54	4.678837e-51
MsG0180001861.01	MsG0580025315.01	0.820782	5.719240e-53	4.568658e-50
MsG0180001861.01	MsG0580025355.01	0.813339	2.690962e-51	1.752588e-48
MsG0180001861.01	MsG0580025392.01	0.833778	4.386180e-56	5.103043e-53
MsG0180001861.01	MsG0580025513.01	0.800877	1.172434e-48	5.520688e-46
MsG0180001861.01	MsG0580025830.01	0.808317	3.286866e-50	1.873448e-47
MsG0180001861.01	MsG0580026211.01	0.831376	1.728505e-55	1.872369e-52
MsG0180001861.01	MsG0580027325.01	0.814215	1.725617e-51	1.150862e-48
MsG0180001861.01	MsG0580028461.01	0.803241	3.830195e-49	1.914995e-46
MsG0180001861.01	MsG0580029816.01	0.837754	4.314010e-57	5.659262e-54
MsG0180001861.01	MsG0680030477.01	0.804491	2.105772e-49	1.087200e-46
MsG0180001861.01	MsG0680030754.01	0.806342	8.619074e-50	4.666639e-47
MsG0180001861.01	MsG0680032840.01	0.814967	1.176611e-51	8.008190e-49
MsG0180001861.01	MsG0780036067.01	0.800052	1.726792e-48	7.961958e-46
MsG0180001861.01	MsG0780036909.01	0.814499	1.493250e-51	1.003685e-48
MsG0180001861.01	MsG0780037655.01	0.820996	5.105533e-53	4.103333e-50
MsG0180001861.01	MsG0780037734.01	0.827111	1.874028e-54	1.791928e-51
MsG0180001861.01	MsG0780038834.01	0.829227	5.792426e-55	5.892305e-52
MsG0180001861.01	MsG0780039287.01	0.809770	1.605891e-50	9.512600e-48
MsG0180001861.01	MsG0780039902.01	0.826546	2.556103e-54	2.404482e-51
MsG0180001861.01	MsG0780039903.01	0.825044	5.807987e-54	5.235328e-51
MsG0180001861.01	MsG0780040072.01	0.819336	1.225543e-52	9.402924e-50
MsG0180001861.01	MsG0780040121.01	0.804255	2.358149e-49	1.210110e-46
MsG0180001861.01	MsG0780040158.01	0.806260	8.968267e-50	4.845924e-47
MsG0180001861.01	MsG0780040159.01	0.840728	7.301749e-58	1.049517e-54
MsG0180001861.01	MsG0780040352.01	0.809762	1.611701e-50	9.545188e-48
MsG0180001861.01	MsG0780040685.01	0.800009	1.762066e-48	8.115677e-46
MsG0180001861.01	MsG0780040884.01	0.820874	5.447422e-53	4.362945e-50
MsG0180001861.01	MsG0780041035.01	0.822957	1.793692e-53	1.523634e-50
MsG0180001861.01	MsG0780041478.01	0.803469	3.434085e-49	1.727098e-46
MsG0180001861.01	MsG0780041492.01	0.805528	1.278078e-49	6.777192e-47
MsG0180001861.01	MsG0780041620.01	0.806934	6.463600e-50	3.553246e-47
MsG0180001861.01	MsG0780041691.01	0.814611	1.410432e-51	9.508256e-49
MsG0180001861.01	MsG0780041809.01	0.825904	3.634575e-54	3.357330e-51
MsG0180001861.01	MsG0880042043.01	0.818285	2.122998e-52	1.582405e-49
MsG0180001861.01	MsG0880042889.01	0.816906	4.343677e-52	3.117512e-49
MsG0180001861.01	MsG0880044387.01	0.818118	2.315792e-52	1.718229e-49
MsG0180001861.01	MsG0880044488.01	0.812739	3.642672e-51	2.334568e-48
MsG0180001861.01	MsG0880044595.01	0.808429	3.110797e-50	1.778307e-47
MsG0180001861.01	MsG0880045324.01	0.804943	1.694652e-49	8.852975e-47
MsG0180001861.01	MsG0880045648.01	0.802458	5.557644e-49	2.723778e-46
MsG0180001861.01	MsG0880045697.01	0.832170	1.101301e-55	1.221154e-52
MsG0180001861.01	MsG0880045778.01	0.814138	1.794934e-51	1.194635e-48
MsG0180001861.01	MsG0880046104.01	0.814858	1.243533e-51	8.438869e-49
MsG0180001861.01	MsG0880046151.01	0.803475	3.425221e-49	1.722881e-46
MsG0180001861.01	MsG0880046219.01	0.810283	1.244823e-50	7.472814e-48
MsG0180001861.01	MsG0880046462.01	0.804374	2.227292e-49	1.146529e-46
MsG0180001861.01	MsG0880046584.01	0.824949	6.116538e-54	5.498288e-51
MsG0180001861.01	MsG0880046639.01	0.838152	3.407521e-57	4.523759e-54
MsG0180001861.01	MsG0880046876.01	0.823012	1.741543e-53	1.481684e-50
MsG0180001861.01	MsG0880047363.01	0.800623	1.321340e-48	6.181445e-46
MsG0180001861.01	MsG0880047578.01	0.839296	1.725131e-57	2.371872e-54
MsG0180001861.01	MsG0880047616.01	0.813130	2.990977e-51	1.936797e-48
MsG0180001861.01	MsG0880047661.01	0.816208	6.225821e-52	4.384050e-49
MsG0180001861.01	MsG0880047669.01	0.804847	1.774332e-49	9.246293e-47
MsG0180001861.01	MsG0880047670.01	0.801091	1.060562e-48	5.021043e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001861.01.T01	MTR_7g113780	97.059	34	1	0	39	72	62	95	1.04e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 62 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAAATGGTCCCCTCTTCTT+TGG	0.153545	1:-28347146	MsG0180001861.01.T01:CDS
GGGATGGGTGGGAGAGGAAG+AGG	0.279567	1:+28347313	None:intergenic
CGGAGGTTGGGGTGTGGGTT+AGG	0.295900	1:+28347200	None:intergenic
CAAATGGTCCCCTCTTCTTT+GGG	0.305845	1:-28347145	MsG0180001861.01.T01:CDS
CTCCATCCTCTCCCTCATTA+TGG	0.310997	1:-28347245	MsG0180001861.01.T01:CDS
GAGAGTGAGGGAGAGATAAA+AGG	0.313559	1:+28347341	None:intergenic
GTGGAGCGGAGGTTGGGGTG+TGG	0.319363	1:+28347194	None:intergenic
CTCTCCCTCATTATGGCTAT+TGG	0.337974	1:-28347238	MsG0180001861.01.T01:CDS
GGACCATTTGGAGGGGTATG+AGG	0.354842	1:+28347158	None:intergenic
GTGGGTTAGGATGGTGAGGG+AGG	0.363724	1:+28347213	None:intergenic
CAGATGGTGGAGCGGAGGTT+GGG	0.365579	1:+28347188	None:intergenic
TGAGGTGGGATGGGTGGGAG+AGG	0.386108	1:+28347307	None:intergenic
GGATGGAGAGGATGAGGTTG+TGG	0.388690	1:+28347260	None:intergenic
TTGAGGAAGTGAGGTGGGAT+GGG	0.392543	1:+28347298	None:intergenic
GGGTGTGGGTTAGGATGGTG+AGG	0.395943	1:+28347209	None:intergenic
AGGAAAGAAAGGGATGAGGC+TGG	0.403020	1:+28347378	None:intergenic
TGGAGCGGAGGTTGGGGTGT+GGG	0.425195	1:+28347195	None:intergenic
GAGGTTGTGGAGTGCGGTGA+AGG	0.426675	1:+28347273	None:intergenic
TGGAAAGAGAGAGGAAAGAA+AGG	0.434953	1:+28347367	None:intergenic
CCAAAGAAGAGGGGACCATT+TGG	0.435019	1:+28347146	None:intergenic
GGTGGGAGAGGAAGAGGAGA+AGG	0.435121	1:+28347319	None:intergenic
TTTGAGGAAGTGAGGTGGGA+TGG	0.438957	1:+28347297	None:intergenic
GGTTGGGGTGTGGGTTAGGA+TGG	0.441521	1:+28347204	None:intergenic
GGGAGAGGATGGAGAGGATG+AGG	0.450488	1:+28347254	None:intergenic
GCAGATGGTGGAGCGGAGGT+TGG	0.452186	1:+28347187	None:intergenic
AGGGATGAGGCTGGAGTGTG+AGG	0.456623	1:+28347387	None:intergenic
GGAAGAGGAGAAGGAGAGTG+AGG	0.458093	1:+28347328	None:intergenic
AGATGGTGGAGCGGAGGTTG+GGG	0.467509	1:+28347189	None:intergenic
CCATTTGGAGGGGTATGAGG+AGG	0.474427	1:+28347161	None:intergenic
AGGAAGTGAGGTGGGATGGG+TGG	0.477567	1:+28347301	None:intergenic
GAAGAGGAGAAGGAGAGTGA+GGG	0.501394	1:+28347329	None:intergenic
GATGAGGCTGGAGTGTGAGG+AGG	0.502414	1:+28347390	None:intergenic
GAGGGAGAGATAAAAGGCTA+TGG	0.508066	1:+28347347	None:intergenic
GGAAAGAGAGAGGAAAGAAA+GGG	0.514238	1:+28347368	None:intergenic
GGAAGTGAGGTGGGATGGGT+GGG	0.514653	1:+28347302	None:intergenic
CCTCCTCATACCCCTCCAAA+TGG	0.515262	1:-28347161	MsG0180001861.01.T01:CDS
GAGGGAAGCAGATGGTGGAG+CGG	0.519215	1:+28347180	None:intergenic
AGATGTAGGCCCAAAGAAGA+GGG	0.520272	1:+28347136	None:intergenic
AGAGAGGAAAGAAAGGGATG+AGG	0.524383	1:+28347374	None:intergenic
AGCCATAATGAGGGAGAGGA+TGG	0.527649	1:+28347243	None:intergenic
AGGGTTTGAGGAAGTGAGGT+GGG	0.541375	1:+28347293	None:intergenic
AAGAAGAGGGGACCATTTGG+AGG	0.542873	1:+28347149	None:intergenic
ATGAGGCTGGAGTGTGAGGA+GGG	0.554548	1:+28347391	None:intergenic
GGAGTGCGGTGAAGGGTTTG+AGG	0.557377	1:+28347281	None:intergenic
AAAAGGCTATGGAAAGAGAG+AGG	0.557470	1:+28347358	None:intergenic
CAATAGCCATAATGAGGGAG+AGG	0.566959	1:+28347239	None:intergenic
AGGTGCCAATAGCCATAATG+AGG	0.568133	1:+28347233	None:intergenic
AGAAGAGGGGACCATTTGGA+GGG	0.569954	1:+28347150	None:intergenic
GTGAAGGGTTTGAGGAAGTG+AGG	0.573391	1:+28347289	None:intergenic
AGGTTGTGGAGTGCGGTGAA+GGG	0.575003	1:+28347274	None:intergenic
AAGGGTTTGAGGAAGTGAGG+TGG	0.580580	1:+28347292	None:intergenic
GGAAGCAGATGGTGGAGCGG+AGG	0.583119	1:+28347183	None:intergenic
AAGATGTAGGCCCAAAGAAG+AGG	0.583857	1:+28347135	None:intergenic
GGTATGAGGAGGGAAGCAGA+TGG	0.584509	1:+28347172	None:intergenic
GAGGATGAGGTTGTGGAGTG+CGG	0.590535	1:+28347267	None:intergenic
ATGAGGAGGGAAGCAGATGG+TGG	0.597449	1:+28347175	None:intergenic
CATTTGGAGGGGTATGAGGA+GGG	0.598396	1:+28347162	None:intergenic
GGTGTGGGTTAGGATGGTGA+GGG	0.620715	1:+28347210	None:intergenic
TAATGAGGGAGAGGATGGAG+AGG	0.644404	1:+28347248	None:intergenic
GGTGCCAATAGCCATAATGA+GGG	0.679843	1:+28347234	None:intergenic
GAAGAGGGGACCATTTGGAG+GGG	0.685233	1:+28347151	None:intergenic
GATGTAGGCCCAAAGAAGAG+GGG	0.691132	1:+28347137	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTTGAGAGGTAGAAGATGT+AGG	+	Chr1:28347392-28347411	None:intergenic	35.0%
	AAAAGGCTATGGAAAGAGAG+AGG	+	Chr1:28347156-28347175	None:intergenic	40.0%
	GGAAAGAGAGAGGAAAGAAA+GGG	+	Chr1:28347146-28347165	None:intergenic	40.0%
	TGGAAAGAGAGAGGAAAGAA+AGG	+	Chr1:28347147-28347166	None:intergenic	40.0%
	AAGATGTAGGCCCAAAGAAG+AGG	+	Chr1:28347379-28347398	None:intergenic	45.0%
	AGAGAGGAAAGAAAGGGATG+AGG	+	Chr1:28347140-28347159	None:intergenic	45.0%
	AGATGTAGGCCCAAAGAAGA+GGG	+	Chr1:28347378-28347397	None:intergenic	45.0%
	AGGTGCCAATAGCCATAATG+AGG	+	Chr1:28347281-28347300	None:intergenic	45.0%
	CAAATGGTCCCCTCTTCTTT+GGG	-	Chr1:28347366-28347385	MsG0180001861.01.T01:CDS	45.0%
	CAATAGCCATAATGAGGGAG+AGG	+	Chr1:28347275-28347294	None:intergenic	45.0%
	CTCTCCCTCATTATGGCTAT+TGG	-	Chr1:28347273-28347292	MsG0180001861.01.T01:CDS	45.0%
	GAGAGTGAGGGAGAGATAAA+AGG	+	Chr1:28347173-28347192	None:intergenic	45.0%
	GAGGGAGAGATAAAAGGCTA+TGG	+	Chr1:28347167-28347186	None:intergenic	45.0%
	GGTGCCAATAGCCATAATGA+GGG	+	Chr1:28347280-28347299	None:intergenic	45.0%
	AAGAAGAGGGGACCATTTGG+AGG	+	Chr1:28347365-28347384	None:intergenic	50.0%
	AAGGGTTTGAGGAAGTGAGG+TGG	+	Chr1:28347222-28347241	None:intergenic	50.0%
	AGAAGAGGGGACCATTTGGA+GGG	+	Chr1:28347364-28347383	None:intergenic	50.0%
	AGCCATAATGAGGGAGAGGA+TGG	+	Chr1:28347271-28347290	None:intergenic	50.0%
	AGGAAAGAAAGGGATGAGGC+TGG	+	Chr1:28347136-28347155	None:intergenic	50.0%
	AGGGTTTGAGGAAGTGAGGT+GGG	+	Chr1:28347221-28347240	None:intergenic	50.0%
	CCAAAGAAGAGGGGACCATT+TGG	+	Chr1:28347368-28347387	None:intergenic	50.0%
	CCAAATGGTCCCCTCTTCTT+TGG	-	Chr1:28347365-28347384	MsG0180001861.01.T01:CDS	50.0%
	CTCCATCCTCTCCCTCATTA+TGG	-	Chr1:28347266-28347285	MsG0180001861.01.T01:CDS	50.0%
	GAAGAGGAGAAGGAGAGTGA+GGG	+	Chr1:28347185-28347204	None:intergenic	50.0%
	GATGTAGGCCCAAAGAAGAG+GGG	+	Chr1:28347377-28347396	None:intergenic	50.0%
	GTGAAGGGTTTGAGGAAGTG+AGG	+	Chr1:28347225-28347244	None:intergenic	50.0%
	TAATGAGGGAGAGGATGGAG+AGG	+	Chr1:28347266-28347285	None:intergenic	50.0%
	TTGAGGAAGTGAGGTGGGAT+GGG	+	Chr1:28347216-28347235	None:intergenic	50.0%
	TTTGAGGAAGTGAGGTGGGA+TGG	+	Chr1:28347217-28347236	None:intergenic	50.0%
!	CATTTGGAGGGGTATGAGGA+GGG	+	Chr1:28347352-28347371	None:intergenic	50.0%
	AGGTTGTGGAGTGCGGTGAA+GGG	+	Chr1:28347240-28347259	None:intergenic	55.0%
	ATGAGGAGGGAAGCAGATGG+TGG	+	Chr1:28347339-28347358	None:intergenic	55.0%
	ATGAGGCTGGAGTGTGAGGA+GGG	+	Chr1:28347123-28347142	None:intergenic	55.0%
	CCTCCTCATACCCCTCCAAA+TGG	-	Chr1:28347350-28347369	MsG0180001861.01.T01:CDS	55.0%
	GAAGAGGGGACCATTTGGAG+GGG	+	Chr1:28347363-28347382	None:intergenic	55.0%
	GAGGATGAGGTTGTGGAGTG+CGG	+	Chr1:28347247-28347266	None:intergenic	55.0%
	GGAAGAGGAGAAGGAGAGTG+AGG	+	Chr1:28347186-28347205	None:intergenic	55.0%
	GGATGGAGAGGATGAGGTTG+TGG	+	Chr1:28347254-28347273	None:intergenic	55.0%
	GGTATGAGGAGGGAAGCAGA+TGG	+	Chr1:28347342-28347361	None:intergenic	55.0%
!	CCATTTGGAGGGGTATGAGG+AGG	+	Chr1:28347353-28347372	None:intergenic	55.0%
!	GGACCATTTGGAGGGGTATG+AGG	+	Chr1:28347356-28347375	None:intergenic	55.0%
!!	GGTGTGGGTTAGGATGGTGA+GGG	+	Chr1:28347304-28347323	None:intergenic	55.0%
	AGGAAGTGAGGTGGGATGGG+TGG	+	Chr1:28347213-28347232	None:intergenic	60.0%
	AGGGATGAGGCTGGAGTGTG+AGG	+	Chr1:28347127-28347146	None:intergenic	60.0%
	GAGGTTGTGGAGTGCGGTGA+AGG	+	Chr1:28347241-28347260	None:intergenic	60.0%
	GATGAGGCTGGAGTGTGAGG+AGG	+	Chr1:28347124-28347143	None:intergenic	60.0%
	GGAAGTGAGGTGGGATGGGT+GGG	+	Chr1:28347212-28347231	None:intergenic	60.0%
	GGAGTGCGGTGAAGGGTTTG+AGG	+	Chr1:28347233-28347252	None:intergenic	60.0%
	GGGAGAGGATGGAGAGGATG+AGG	+	Chr1:28347260-28347279	None:intergenic	60.0%
	GGTGGGAGAGGAAGAGGAGA+AGG	+	Chr1:28347195-28347214	None:intergenic	60.0%
!!	AGATGGTGGAGCGGAGGTTG+GGG	+	Chr1:28347325-28347344	None:intergenic	60.0%
!!	CAGATGGTGGAGCGGAGGTT+GGG	+	Chr1:28347326-28347345	None:intergenic	60.0%
!!	GAGGGAAGCAGATGGTGGAG+CGG	+	Chr1:28347334-28347353	None:intergenic	60.0%
!!	GGGTGTGGGTTAGGATGGTG+AGG	+	Chr1:28347305-28347324	None:intergenic	60.0%
!!	GGTTGGGGTGTGGGTTAGGA+TGG	+	Chr1:28347310-28347329	None:intergenic	60.0%
!!	GTGGGTTAGGATGGTGAGGG+AGG	+	Chr1:28347301-28347320	None:intergenic	60.0%
	CGGAGGTTGGGGTGTGGGTT+AGG	+	Chr1:28347314-28347333	None:intergenic	65.0%
	GGGATGGGTGGGAGAGGAAG+AGG	+	Chr1:28347201-28347220	None:intergenic	65.0%
	TGAGGTGGGATGGGTGGGAG+AGG	+	Chr1:28347207-28347226	None:intergenic	65.0%
	TGGAGCGGAGGTTGGGGTGT+GGG	+	Chr1:28347319-28347338	None:intergenic	65.0%
!!	GCAGATGGTGGAGCGGAGGT+TGG	+	Chr1:28347327-28347346	None:intergenic	65.0%
!!	GGAAGCAGATGGTGGAGCGG+AGG	+	Chr1:28347331-28347350	None:intergenic	65.0%
	GTGGAGCGGAGGTTGGGGTG+TGG	+	Chr1:28347320-28347339	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	28347114	28347419	28347114	ID=MsG0180001861.01;Name=MsG0180001861.01
Chr1	mRNA	28347114	28347419	28347114	ID=MsG0180001861.01.T01;Parent=MsG0180001861.01;Name=MsG0180001861.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|101
Chr1	exon	28347114	28347419	28347114	ID=MsG0180001861.01.T01:exon:18844;Parent=MsG0180001861.01.T01
Chr1	CDS	28347114	28347419	28347114	ID=MsG0180001861.01.T01:cds;Parent=MsG0180001861.01.T01

Gene Sequence	>MsG0180001861.01.T01 ATGGAACCCTCCTCACACTCCAGCCTCATCCCTTTCTTTCCTCTCTCTTTCCATAGCCTTTTATCTCTCCCTCACTCTCCTTCTCCTCTTCCTCTCCCACCCATCCCACCTCACTTCCTCAAACCCTTCACCGCACTCCACAACCTCATCCTCTCCATCCTCTCCCTCATTATGGCTATTGGCACCTCCCTCACCATCCTAACCCACACCCCAACCTCCGCTCCACCATCTGCTTCCCTCCTCATACCCCTCCAAATGGTCCCCTCTTCTTTGGGCCTACATCTTCTACCTCTCAAAATATCTTGA
Protein sequence	>MsG0180001861.01.T01 MEPSSHSSLIPFFPLSFHSLLSLPHSPSPLPLPPIPPHFLKPFTALHNLILSILSLIMAIGTSLTILTHTPTSAPPSASLLIPLQMVPSSLGLHLLPLKIS*