AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029816.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029816.01.T01 MTR_5g093240 82.836 134 8 2 1 134 1 119 1.17e-73 215
MsG0580029816.01.T01 MTR_5g093240 89.024 82 6 1 1 82 1 79 9.10e-46 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTCAGTAAGTTCATTA+AGG 0.284385 5:-102317172 MsG0580029816.01.T01:CDS
CTTCTGCATCATCAACCTTC+TGG 0.304356 5:+102316837 None:intergenic
CGCAGCAGCAACATTATCTA+AGG 0.361910 5:-102317259 MsG0580029816.01.T01:CDS
GCAGCAACAACAATGATATC+TGG 0.364461 5:+102317137 None:intergenic
GCACAACACAAAACTACTTT+GGG 0.366214 5:-102317231 MsG0580029816.01.T01:CDS
AGAAAAGAACCGAACCTTAC+TGG 0.375391 5:+102317074 None:intergenic
TGTTCACTTGGGATAATGTT+TGG 0.377532 5:+102317406 None:intergenic
GTTCACTTGGGATAATGTTT+GGG 0.382397 5:+102317407 None:intergenic
TCTTCAACAAGATATAGTTT+TGG 0.385269 5:+102317376 None:intergenic
ATGGATCGGCGGGTTGTGGC+AGG 0.389363 5:+102317108 None:intergenic
AAAATTTACAACTACACATA+AGG 0.394107 5:+102317471 None:intergenic
AAATACTTGCTTATAAAATC+TGG 0.401289 5:+102316884 None:intergenic
GAAGCAATGAAGAGGGGAGG+TGG 0.407582 5:+102317303 None:intergenic
CTATGTGGCATGGTCTTGCT+TGG 0.417895 5:+102317203 None:intergenic
TTTGGTTCACTGTGTTCACT+TGG 0.441499 5:+102317394 None:intergenic
AGCACAACACAAAACTACTT+TGG 0.452055 5:-102317232 MsG0580029816.01.T01:CDS
CAATGATATCTGGAATTGCC+TGG 0.462076 5:+102317147 None:intergenic
TGAAGTATACTGATATAAGC+AGG 0.464588 5:+102316924 None:intergenic
CTTACTGGGAGAAAAGTAGA+TGG 0.479013 5:+102317089 None:intergenic
GTAGATGGATCGGCGGGTTG+TGG 0.490348 5:+102317104 None:intergenic
GAAAAGAACCGAACCTTACT+GGG 0.530829 5:+102317075 None:intergenic
CTTAGATAATGTTGCTGCTG+CGG 0.540896 5:+102317260 None:intergenic
TTGGGATAATGTTTGGGAGG+AGG 0.545339 5:+102317413 None:intergenic
TTGGTTCACTGTGTTCACTT+GGG 0.550658 5:+102317395 None:intergenic
TTTGGATTTGATAAACTAGA+GGG 0.553590 5:-102316863 MsG0580029816.01.T01:CDS
CACTTGGGATAATGTTTGGG+AGG 0.556445 5:+102317410 None:intergenic
TGCATGAGAAGCAATGAAGA+GGG 0.563172 5:+102317296 None:intergenic
TGGATCGGCGGGTTGTGGCA+GGG 0.568272 5:+102317109 None:intergenic
AGAAAAGTAGATGGATCGGC+GGG 0.588206 5:+102317098 None:intergenic
AATGATATCTGGAATTGCCT+GGG 0.588413 5:+102317148 None:intergenic
GAAGAAAAGTGCTTACTATG+TGG 0.588606 5:+102317188 None:intergenic
TGGGAGAAAAGTAGATGGAT+CGG 0.598368 5:+102317094 None:intergenic
GCAGCAACATTATCTAAGGA+TGG 0.606428 5:-102317255 MsG0580029816.01.T01:CDS
AGTAAGTTCATTAAGGACCC+AGG 0.609846 5:-102317165 MsG0580029816.01.T01:CDS
TAAACTAGAGGGAGACCAGA+AGG 0.616294 5:-102316852 MsG0580029816.01.T01:CDS
TTAGTTAAACTAGTTATGCG+TGG 0.617347 5:+102317345 None:intergenic
AAAGTGCTTACTATGTGGCA+TGG 0.619717 5:+102317193 None:intergenic
GAAGTATACTGATATAAGCA+GGG 0.632422 5:+102316925 None:intergenic
GGGGAGGTGGAGACAAGTCA+TGG 0.642574 5:+102317316 None:intergenic
ATGCATGAGAAGCAATGAAG+AGG 0.647708 5:+102317295 None:intergenic
TGAGAAGCAATGAAGAGGGG+AGG 0.663602 5:+102317300 None:intergenic
GAGAAAAGTAGATGGATCGG+CGG 0.670606 5:+102317097 None:intergenic
GCATGAGAAGCAATGAAGAG+GGG 0.705476 5:+102317297 None:intergenic
CACAACACAAAACTACTTTG+GGG 0.712340 5:-102317230 MsG0580029816.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTGTAAATTTTAAATGTA+AGG - Chr5:102316858-102316877 MsG0580029816.01.T01:CDS 15.0%
!!! GTTGTAAATTTTAAATGTAA+GGG - Chr5:102316859-102316878 MsG0580029816.01.T01:CDS 15.0%
!!! TAGTATTTTTTTTTTACACT+AGG - Chr5:102317304-102317323 MsG0580029816.01.T01:CDS 15.0%
!! AAAATTTACAACTACACATA+AGG + Chr5:102316851-102316870 None:intergenic 20.0%
!! AAATACTTGCTTATAAAATC+TGG + Chr5:102317438-102317457 None:intergenic 20.0%
!!! GATTTTATAAGCAAGTATTT+TGG - Chr5:102317438-102317457 MsG0580029816.01.T01:five_prime_UTR 20.0%
! TTTCTTCAGTAAGTTCATTA+AGG - Chr5:102317147-102317166 MsG0580029816.01.T01:CDS 25.0%
! TTTGGATTTGATAAACTAGA+GGG - Chr5:102317456-102317475 MsG0580029816.01.T01:five_prime_UTR 25.0%
!! TCTTCAACAAGATATAGTTT+TGG + Chr5:102316946-102316965 None:intergenic 25.0%
!! TTTTGGATTTGATAAACTAG+AGG - Chr5:102317455-102317474 MsG0580029816.01.T01:five_prime_UTR 25.0%
!!! TGTTCATTATGTCATTTTCA+TGG - Chr5:102317274-102317293 MsG0580029816.01.T01:CDS 25.0%
GAAGTATACTGATATAAGCA+GGG + Chr5:102317397-102317416 None:intergenic 30.0%
TGAAGTATACTGATATAAGC+AGG + Chr5:102317398-102317417 None:intergenic 30.0%
TTAGTTAAACTAGTTATGCG+TGG + Chr5:102316977-102316996 None:intergenic 30.0%
ACAAGCTGCAAACTTAAACA+AGG - Chr5:102317334-102317353 MsG0580029816.01.T01:exon 35.0%
AGCACAACACAAAACTACTT+TGG - Chr5:102317087-102317106 MsG0580029816.01.T01:intron 35.0%
CACAACACAAAACTACTTTG+GGG - Chr5:102317089-102317108 MsG0580029816.01.T01:CDS 35.0%
GCACAACACAAAACTACTTT+GGG - Chr5:102317088-102317107 MsG0580029816.01.T01:intron 35.0%
GTTCACTTGGGATAATGTTT+GGG + Chr5:102316915-102316934 None:intergenic 35.0%
TGTTCACTTGGGATAATGTT+TGG + Chr5:102316916-102316935 None:intergenic 35.0%
! AATGATATCTGGAATTGCCT+GGG + Chr5:102317174-102317193 None:intergenic 35.0%
!! GAAGAAAAGTGCTTACTATG+TGG + Chr5:102317134-102317153 None:intergenic 35.0%
AGAAAAGAACCGAACCTTAC+TGG + Chr5:102317248-102317267 None:intergenic 40.0%
AGTAAGTTCATTAAGGACCC+AGG - Chr5:102317154-102317173 MsG0580029816.01.T01:CDS 40.0%
ATGCATGAGAAGCAATGAAG+AGG + Chr5:102317027-102317046 None:intergenic 40.0%
CTTACTGGGAGAAAAGTAGA+TGG + Chr5:102317233-102317252 None:intergenic 40.0%
CTTAGATAATGTTGCTGCTG+CGG + Chr5:102317062-102317081 None:intergenic 40.0%
GAAAAGAACCGAACCTTACT+GGG + Chr5:102317247-102317266 None:intergenic 40.0%
GCAGCAACAACAATGATATC+TGG + Chr5:102317185-102317204 None:intergenic 40.0%
GCAGCAACATTATCTAAGGA+TGG - Chr5:102317064-102317083 MsG0580029816.01.T01:intron 40.0%
TGCATGAGAAGCAATGAAGA+GGG + Chr5:102317026-102317045 None:intergenic 40.0%
TGGGAGAAAAGTAGATGGAT+CGG + Chr5:102317228-102317247 None:intergenic 40.0%
TTGGTTCACTGTGTTCACTT+GGG + Chr5:102316927-102316946 None:intergenic 40.0%
! AAAGTGCTTACTATGTGGCA+TGG + Chr5:102317129-102317148 None:intergenic 40.0%
! ACTTTTCTCCCAGTAAGGTT+CGG - Chr5:102317236-102317255 MsG0580029816.01.T01:CDS 40.0%
! CAATGATATCTGGAATTGCC+TGG + Chr5:102317175-102317194 None:intergenic 40.0%
! TTTGGTTCACTGTGTTCACT+TGG + Chr5:102316928-102316947 None:intergenic 40.0%
!! CATCTACTTTTCTCCCAGTA+AGG - Chr5:102317231-102317250 MsG0580029816.01.T01:CDS 40.0%
AGAAAAGTAGATGGATCGGC+GGG + Chr5:102317224-102317243 None:intergenic 45.0%
CACTTGGGATAATGTTTGGG+AGG + Chr5:102316912-102316931 None:intergenic 45.0%
CGCAGCAGCAACATTATCTA+AGG - Chr5:102317060-102317079 MsG0580029816.01.T01:intron 45.0%
CTTCTGCATCATCAACCTTC+TGG + Chr5:102317485-102317504 None:intergenic 45.0%
GAGAAAAGTAGATGGATCGG+CGG + Chr5:102317225-102317244 None:intergenic 45.0%
GCATGAGAAGCAATGAAGAG+GGG + Chr5:102317025-102317044 None:intergenic 45.0%
TAAACTAGAGGGAGACCAGA+AGG - Chr5:102317467-102317486 MsG0580029816.01.T01:five_prime_UTR 45.0%
TTGGGATAATGTTTGGGAGG+AGG + Chr5:102316909-102316928 None:intergenic 45.0%
TGAGAAGCAATGAAGAGGGG+AGG + Chr5:102317022-102317041 None:intergenic 50.0%
! CTATGTGGCATGGTCTTGCT+TGG + Chr5:102317119-102317138 None:intergenic 50.0%
GAAGCAATGAAGAGGGGAGG+TGG + Chr5:102317019-102317038 None:intergenic 55.0%
GGGGAGGTGGAGACAAGTCA+TGG + Chr5:102317006-102317025 None:intergenic 60.0%
!! GTAGATGGATCGGCGGGTTG+TGG + Chr5:102317218-102317237 None:intergenic 60.0%
!! ATGGATCGGCGGGTTGTGGC+AGG + Chr5:102317214-102317233 None:intergenic 65.0%
!! TGGATCGGCGGGTTGTGGCA+GGG + Chr5:102317213-102317232 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 102316829 102317512 102316829 ID=MsG0580029816.01;Name=MsG0580029816.01
Chr5 mRNA 102316829 102317512 102316829 ID=MsG0580029816.01.T01;Parent=MsG0580029816.01;Name=MsG0580029816.01.T01;_AED=0.25;_eAED=0.27;_QI=176|0|0.5|1|0|0|2|0|134
Chr5 exon 102316829 102316985 102316829 ID=MsG0580029816.01.T01:exon:6516;Parent=MsG0580029816.01.T01
Chr5 exon 102317089 102317512 102317089 ID=MsG0580029816.01.T01:exon:6515;Parent=MsG0580029816.01.T01
Chr5 five_prime_UTR 102317337 102317512 102317337 ID=MsG0580029816.01.T01:five_prime_utr;Parent=MsG0580029816.01.T01
Chr5 CDS 102317089 102317336 102317089 ID=MsG0580029816.01.T01:cds;Parent=MsG0580029816.01.T01
Chr5 CDS 102316829 102316985 102316829 ID=MsG0580029816.01.T01:cds;Parent=MsG0580029816.01.T01
Gene Sequence

>MsG0580029816.01.T01

ATGACTTGTCTCCACCTCCCCTCTTCATTGCTTCTCATGCATACAAAACTCAAACCGCAGCAGCAACATTATCTAAGGATGGAGCACAACACAAAACTACTTTGGGGAGAACCAAGCAAGACCATGCCACATAGTAAGCACTTTTCTTCAGTAAGTTCATTAAGGACCCAGGCAATTCCAGATATCATTGTTGTTGCTGCTACTTCCCTGCCACAACCCGCCGATCCATCTACTTTTCTCCCAGTAAGGTTGTTTTCTGCTCATAATTTGTTTATACATTGCAGTGCCCTGCTTATATCAGTATACTTCATAGCAAATTTCGTCGTGCCAGATTTTATAAGCAAGTATTTTGGATTTGATAAACTAGAGGGAGACCAGAAGGTTGATGATGCAGAAGATAAATAG

Protein sequence

>MsG0580029816.01.T01

MTCLHLPSSLLLMHTKLKPQQQHYLRMEHNTKLLWGEPSKTMPHSKHFSSVSSLRTQAIPDIIVVAATSLPQPADPSTFLPVRLFSAHNLFIHCSALLISVYFIANFVVPDFISKYFGFDKLEGDQKVDDAEDK*