Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029816.01.T01 | XP_003617588.1 | 82.836 | 134 | 8 | 2 | 1 | 134 | 1 | 119 | 9.69E-70 | 215 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0580029816.01 | 0.867174 | 1.661790e-65 | 5.746985e-62 |
| MsG0080047945.01 | MsG0580029816.01 | 0.847674 | 9.959490e-60 | 1.783767e-56 |
| MsG0080047955.01 | MsG0580029816.01 | 0.828906 | 6.930187e-55 | 6.982078e-52 |
| MsG0080047956.01 | MsG0580029816.01 | 0.847615 | 1.033544e-59 | 1.847445e-56 |
| MsG0080048036.01 | MsG0580029816.01 | -0.816394 | 5.656023e-52 | 4.002990e-49 |
| MsG0080048059.01 | MsG0580029816.01 | 0.881828 | 1.734415e-70 | 1.031550e-66 |
| MsG0080048528.01 | MsG0580029816.01 | 0.835892 | 1.288043e-56 | 1.596640e-53 |
| MsG0080048611.01 | MsG0580029816.01 | 0.838522 | 2.736146e-57 | 3.674091e-54 |
| MsG0080048776.01 | MsG0580029816.01 | 0.836291 | 1.020152e-56 | 1.280119e-53 |
| MsG0080048777.01 | MsG0580029816.01 | 0.805595 | 1.237304e-49 | 6.572373e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029816.01.T01 | MTR_5g093240 | 82.836 | 134 | 8 | 2 | 1 | 134 | 1 | 119 | 1.17e-73 | 215 |
| MsG0580029816.01.T01 | MTR_5g093240 | 89.024 | 82 | 6 | 1 | 1 | 82 | 1 | 79 | 9.10e-46 | 144 |
Find 44 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTTACAACTACACATA+AGG | 0.394107 | 5:+102317471 | None:intergenic |
| AAAGTGCTTACTATGTGGCA+TGG | 0.619717 | 5:+102317193 | None:intergenic |
| AAATACTTGCTTATAAAATC+TGG | 0.401289 | 5:+102316884 | None:intergenic |
| AATGATATCTGGAATTGCCT+GGG | 0.588413 | 5:+102317148 | None:intergenic |
| AGAAAAGAACCGAACCTTAC+TGG | 0.375391 | 5:+102317074 | None:intergenic |
| AGAAAAGTAGATGGATCGGC+GGG | 0.588206 | 5:+102317098 | None:intergenic |
| AGCACAACACAAAACTACTT+TGG | 0.452055 | 5:-102317232 | MsG0580029816.01.T01:CDS |
| AGTAAGTTCATTAAGGACCC+AGG | 0.609846 | 5:-102317165 | MsG0580029816.01.T01:CDS |
| ATGCATGAGAAGCAATGAAG+AGG | 0.647708 | 5:+102317295 | None:intergenic |
| ATGGATCGGCGGGTTGTGGC+AGG | 0.389363 | 5:+102317108 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAGTATACTGATATAAGCA+GGG | + | Chr5:102317397-102317416 | None:intergenic | 30.0% | |
| TGAAGTATACTGATATAAGC+AGG | + | Chr5:102317398-102317417 | None:intergenic | 30.0% | |
| TTAGTTAAACTAGTTATGCG+TGG | + | Chr5:102316977-102316996 | None:intergenic | 30.0% | |
| ACAAGCTGCAAACTTAAACA+AGG | - | Chr5:102317334-102317353 | MsG0580029816.01.T01:exon | 35.0% | |
| AGCACAACACAAAACTACTT+TGG | - | Chr5:102317087-102317106 | MsG0580029816.01.T01:intron | 35.0% | |
| CACAACACAAAACTACTTTG+GGG | - | Chr5:102317089-102317108 | MsG0580029816.01.T01:CDS | 35.0% | |
| GCACAACACAAAACTACTTT+GGG | - | Chr5:102317088-102317107 | MsG0580029816.01.T01:intron | 35.0% | |
| GTTCACTTGGGATAATGTTT+GGG | + | Chr5:102316915-102316934 | None:intergenic | 35.0% | |
| TGTTCACTTGGGATAATGTT+TGG | + | Chr5:102316916-102316935 | None:intergenic | 35.0% | |
| AGAAAAGAACCGAACCTTAC+TGG | + | Chr5:102317248-102317267 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 102316829 | 102317512 | 102316829 | ID=MsG0580029816.01;Name=MsG0580029816.01 |
| Chr5 | mRNA | 102316829 | 102317512 | 102316829 | ID=MsG0580029816.01.T01;Parent=MsG0580029816.01;Name=MsG0580029816.01.T01;_AED=0.25;_eAED=0.27;_QI=176|0|0.5|1|0|0|2|0|134 |
| Chr5 | exon | 102316829 | 102316985 | 102316829 | ID=MsG0580029816.01.T01:exon:6516;Parent=MsG0580029816.01.T01 |
| Chr5 | exon | 102317089 | 102317512 | 102317089 | ID=MsG0580029816.01.T01:exon:6515;Parent=MsG0580029816.01.T01 |
| Chr5 | five_prime_UTR | 102317337 | 102317512 | 102317337 | ID=MsG0580029816.01.T01:five_prime_utr;Parent=MsG0580029816.01.T01 |
| Chr5 | CDS | 102317089 | 102317336 | 102317089 | ID=MsG0580029816.01.T01:cds;Parent=MsG0580029816.01.T01 |
| Chr5 | CDS | 102316829 | 102316985 | 102316829 | ID=MsG0580029816.01.T01:cds;Parent=MsG0580029816.01.T01 |
| Gene Sequence |
>MsG0580029816.01.T01 ATGACTTGTCTCCACCTCCCCTCTTCATTGCTTCTCATGCATACAAAACTCAAACCGCAGCAGCAACATTATCTAAGGATGGAGCACAACACAAAACTACTTTGGGGAGAACCAAGCAAGACCATGCCACATAGTAAGCACTTTTCTTCAGTAAGTTCATTAAGGACCCAGGCAATTCCAGATATCATTGTTGTTGCTGCTACTTCCCTGCCACAACCCGCCGATCCATCTACTTTTCTCCCAGTAAGGTTGTTTTCTGCTCATAATTTGTTTATACATTGCAGTGCCCTGCTTATATCAGTATACTTCATAGCAAATTTCGTCGTGCCAGATTTTATAAGCAAGTATTTTGGATTTGATAAACTAGAGGGAGACCAGAAGGTTGATGATGCAGAAGATAAATAG |
| Protein sequence |
>MsG0580029816.01.T01 MTCLHLPSSLLLMHTKLKPQQQHYLRMEHNTKLLWGEPSKTMPHSKHFSSVSSLRTQAIPDIIVVAATSLPQPADPSTFLPVRLFSAHNLFIHCSALLISVYFIANFVVPDFISKYFGFDKLEGDQKVDDAEDK* |
