AlfalfaGEDB | MsG0380011632.01

M. sativa cultivar ZhongmuNo.1 / MsG0380011632.01

KEGG: K06199
GO: GO:0016021
PFAM: PF02537

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011632.01.T01	XP_003598118.2	88.571	315	17	2	1	296	1	315	0	528

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011632.01.T01	G7IV96	88.571	315	17	2	1	296	1	315	0.0	528

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047910.01	MsG0380011632.01	0.840608	7.847781e-58	1.123731e-54
MsG0080047945.01	MsG0380011632.01	0.804271	2.339871e-49	1.201254e-46
MsG0080047955.01	MsG0380011632.01	0.825119	5.575540e-54	5.036341e-51
MsG0080047956.01	MsG0380011632.01	0.823505	1.336331e-53	1.152822e-50
MsG0080047989.01	MsG0380011632.01	0.812296	4.552488e-51	2.883613e-48
MsG0080048068.01	MsG0380011632.01	0.841088	5.873234e-58	8.535442e-55
MsG0080048085.01	MsG0380011632.01	0.804569	2.028111e-49	1.049242e-46
MsG0080048191.01	MsG0380011632.01	0.851526	8.379677e-61	1.700909e-57
MsG0080048198.01	MsG0380011632.01	0.855830	4.849467e-62	1.134266e-58
MsG0080048273.01	MsG0380011632.01	0.841533	4.484612e-58	6.609109e-55

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011632.01.T01	MTR_3g007470	88.571	315	17	2	1	296	1	315	0.0	528
MsG0380011632.01.T01	MTR_3g072830	58.389	298	105	6	12	292	79	374	6.57e-110	326
MsG0380011632.01.T01	MTR_3g072840	61.011	277	91	5	35	296	103	377	1.98e-96	291

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011632.01.T01	AT2G41705	50.000	308	120	7	7	296	100	391	1.93e-83	259
MsG0380011632.01.T01	AT2G41705	50.000	308	120	7	7	296	100	391	1.93e-83	259
MsG0380011632.01.T01	AT2G41705	50.000	308	120	7	7	296	100	391	1.93e-83	259
MsG0380011632.01.T01	AT2G41705	50.000	308	120	7	7	296	100	391	1.93e-83	259

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Find 64 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGCTAAAGGTTTCAAG+TGG	0.608710	3:+2732174	MsG0380011632.01.T01:CDS
AAAGTAGAGACCATATTAGA+GGG	0.599374	3:-2731162	None:intergenic
AACAAATTGCAGCTTCATCT+TGG	0.384111	3:+2729876	MsG0380011632.01.T01:CDS
AACTTAGTGTTACTGGGAAT+TGG	0.333110	3:+2731480	MsG0380011632.01.T01:CDS
AAGACCAAGGCAAAGGTGGA+AGG	0.581372	3:+2732245	MsG0380011632.01.T01:CDS
AAGAGGGGACATTTGTTCAG+AGG	0.563333	3:-2729916	None:intergenic
AAGTTATTTGGCCCTAGTGT+TGG	0.545174	3:+2731099	MsG0380011632.01.T01:CDS
AATTGGCTCTTTGTTGTGCT+TGG	0.359346	3:+2731497	MsG0380011632.01.T01:CDS
ACATCACTTAGTAGTTATGC+TGG	0.551387	3:+2732274	MsG0380011632.01.T01:CDS
AGATTCTGAAGACATACAAC+AGG	0.496202	3:+2729956	MsG0380011632.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATTTAGGAGTGAGGAA+GGG	-	Chr3:2731191-2731210	None:intergenic	30.0%
AAAGTAGAGACCATATTAGA+GGG	-	Chr3:2731165-2731184	None:intergenic	30.0%
ATGATTGCTGAATTCAAGAA+TGG	+	Chr3:2732344-2732363	MsG0380011632.01.T01:CDS	30.0%
CTAATCCATGTCCATTTAAT+CGG	-	Chr3:2732439-2732458	None:intergenic	30.0%
GGTATTAACAAGATATACTC+TGG	+	Chr3:2731074-2731093	MsG0380011632.01.T01:intron	30.0%
TAATCCATGTCCATTTAATC+GGG	-	Chr3:2732438-2732457	None:intergenic	30.0%
TATACTCTGGACAAGTTATT+TGG	+	Chr3:2731087-2731106	MsG0380011632.01.T01:CDS	30.0%
TATTTCCATTTGACCATTAC+TGG	+	Chr3:2730368-2730387	MsG0380011632.01.T01:intron	30.0%
TGAGGATTAAGATTGAATGA+CGG	-	Chr3:2730068-2730087	None:intergenic	30.0%
TGTTGCATTCCTTTATCTTT+GGG	+	Chr3:2730951-2730970	MsG0380011632.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	2729750	2732568	2729750	ID=MsG0380011632.01;Name=MsG0380011632.01
Chr3	mRNA	2729750	2732568	2729750	ID=MsG0380011632.01.T01;Parent=MsG0380011632.01;Name=MsG0380011632.01.T01;_AED=0.42;_eAED=0.43;_QI=105\|0.25\|0.2\|1\|1\|1\|5\|0\|297
Chr3	exon	2729750	2729977	2729750	ID=MsG0380011632.01.T01:exon:835;Parent=MsG0380011632.01.T01
Chr3	exon	2730217	2730300	2730217	ID=MsG0380011632.01.T01:exon:836;Parent=MsG0380011632.01.T01
Chr3	exon	2731075	2731173	2731075	ID=MsG0380011632.01.T01:exon:837;Parent=MsG0380011632.01.T01
Chr3	exon	2731338	2731530	2731338	ID=MsG0380011632.01.T01:exon:838;Parent=MsG0380011632.01.T01
Chr3	exon	2732174	2732568	2732174	ID=MsG0380011632.01.T01:exon:839;Parent=MsG0380011632.01.T01
Chr3	five_prime_UTR	2729750	2729854	2729750	ID=MsG0380011632.01.T01:five_prime_utr;Parent=MsG0380011632.01.T01
Chr3	CDS	2729855	2729977	2729855	ID=MsG0380011632.01.T01:cds;Parent=MsG0380011632.01.T01
Chr3	CDS	2730217	2730300	2730217	ID=MsG0380011632.01.T01:cds;Parent=MsG0380011632.01.T01
Chr3	CDS	2731075	2731173	2731075	ID=MsG0380011632.01.T01:cds;Parent=MsG0380011632.01.T01
Chr3	CDS	2731338	2731530	2731338	ID=MsG0380011632.01.T01:cds;Parent=MsG0380011632.01.T01
Chr3	CDS	2732174	2732568	2732174	ID=MsG0380011632.01.T01:cds;Parent=MsG0380011632.01.T01

Gene Sequence

>MsG0380011632.01.T01

ATGGAGCATGATGTTGGAGTTAACAAATTGCAGCTTCATCTTGGTGCATCCAATTCTGTCTCCTCTGAACAAATGTCCCCTCTTTTAAGCATTGAAGGAGAAGATTCTGAAGACATACAACAGTTGCAGGAACCTAGTAAAGAATTGCCAAAGCTACTGGATAGTGCTTCATATTTGATTCATTTGGCTGTTTTTGGTATTCTCGGGGTATTAACAAGATATACTCTGGACAAGTTATTTGGCCCTAGTGTTGGTCATGTGACTAGTGACCAAACTATTCTTTACCTTGACCTTCCCTCTAATATGGTTGGTTCTTTTCTGATGGGATGGTTTGGTGTTGTATTCAAAGAAGACCTATCAAATGTGTCCAAGTATCTGGCTGTTGCACTAACAACTGGTTACTTAGGGAGTCTAACAACGTTCAGCGGCTGGAATCAGAAAATGCTTGAACTTAGTGTTACTGGGAATTGGCTCTTTGTTGTGCTTGGCTTTCTAATAGAAACAGCTAAAGGTTTCAAGTGGCTTCTTATAAGGCTAAACGTGACTTCACGGAGTGAAGCTAGTAGAGCCAAGACCAAGGCAAAGGTGGAAGGCTTCAAACATCACTTAGTAGTTATGCTGGTGTTTTTGCTGATTTTAGGCTTGTTATGGGGTATTAGCGGTGTATTGATGATTGCTGAATTCAAGAATGGTGAAAATGCTTATCTGTGGATCGCTTGCATTATTGGACCTATTGGTGTGTGGATTAGATGGATTTTATCCCGATTAAATGGACATGGATTAGGATCAAGAGGTTTGTTGAATTGGATGCGATTTGGAACTCTAACTGCAAATGTCTCTGCTGCTTGTATCATGGCTGCACTTGCTACAACAAAGATATATGTGAGTTTCTAG

Protein sequence

>MsG0380011632.01.T01

MEHDVGVNKLQLHLGASNSVSSEQMSPLLSIEGEDSEDIQQLQEPSKELPKLLDSASYLIHLAVFGILGVLTRYTLDKLFGPSVGHVTSDQTILYLDLPSNMVGSFLMGWFGVVFKEDLSNVSKYLAVALTTGYLGSLTTFSGWNQKMLELSVTGNWLFVVLGFLIETAKGFKWLLIRLNVTSRSEASRAKTKAKVEGFKHHLVVMLVFLLILGLLWGISGVLMIAEFKNGENAYLWIACIIGPIGVWIRWILSRLNGHGLGSRGLLNWMRFGTLTANVSAACIMAALATTKIYVSF*