AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380011896.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011896.01.T01 MTR_3g010000 86.486 111 3 1 12 122 52 150 6.94e-65 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011896.01.T01 AT1G55670 86.087 115 16 0 8 122 46 160 2.87e-71 210

Find 16 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTATGATCCAAGTTTCTT+TGG 0.281064 3:+7607619 MsG0380011896.01.T01:CDS
TTGCTCAGGTAAGCCTTGTT+TGG 0.325198 3:-7607437 None:intergenic
TATTGTTGCTTACTATATCT+TGG 0.334380 3:+7607582 MsG0380011896.01.T01:CDS
GTTAAAACCAACAGGATCAT+TGG 0.338510 3:-7607524 None:intergenic
CAAGGCTTACCTGAGCAAAA+TGG 0.416510 3:+7607442 MsG0380011896.01.T01:CDS
TCAACAAGGTTAAAACCAAC+AGG 0.475786 3:-7607532 None:intergenic
CTTAAATCCAATGATCCTGT+TGG 0.489692 3:+7607517 MsG0380011896.01.T01:CDS
AAATATCAACCAAAGAAACT+TGG 0.505006 3:-7607628 None:intergenic
CTTAAGCTTATCACAAGTGA+AGG 0.506695 3:-7607352 None:intergenic
AGCTGGTGACAAACGTGCTA+AGG 0.525842 3:+7607480 MsG0380011896.01.T01:CDS
ATCTTGGCTACTTCTAGCAA+TGG 0.561786 3:+7607598 MsG0380011896.01.T01:CDS
AGAGAAAATGTAGCCAAACA+AGG 0.605177 3:+7607424 MsG0380011896.01.T01:CDS
CTTGTGATAAGCTTAAGCAC+TGG 0.608219 3:+7607358 MsG0380011896.01.T01:CDS
TTAAGCTTATCACAAGTGAA+GGG 0.640842 3:-7607351 None:intergenic
GGTGTGACACACTTTGAAGC+TGG 0.654419 3:+7607463 MsG0380011896.01.T01:CDS
CCCAAGCCAAAACATCAACA+AGG 0.663918 3:-7607546 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTTGCTTACTATATCT+TGG + Chr3:7607582-7607601 MsG0380011896.01.T01:CDS 25.0%
!! TTTTAACCTTGTTGATGTTT+TGG + Chr3:7607540-7607559 MsG0380011896.01.T01:CDS 25.0%
TTAAGCTTATCACAAGTGAA+GGG - Chr3:7607354-7607373 None:intergenic 30.0%
! ACTTGAAAATCTCTTTTGCT+TGG - Chr3:7607308-7607327 None:intergenic 30.0%
!!! ACTGGTCTTTCATTGTTTTT+AGG + Chr3:7607376-7607395 MsG0380011896.01.T01:CDS 30.0%
!!! GTCTTTCATTGTTTTTAGGA+AGG + Chr3:7607380-7607399 MsG0380011896.01.T01:CDS 30.0%
AGAGAAAATGTAGCCAAACA+AGG + Chr3:7607424-7607443 MsG0380011896.01.T01:CDS 35.0%
CTTAAATCCAATGATCCTGT+TGG + Chr3:7607517-7607536 MsG0380011896.01.T01:CDS 35.0%
CTTAAGCTTATCACAAGTGA+AGG - Chr3:7607355-7607374 None:intergenic 35.0%
GGTTATGATCCAAGTTTCTT+TGG + Chr3:7607619-7607638 MsG0380011896.01.T01:CDS 35.0%
GTTAAAACCAACAGGATCAT+TGG - Chr3:7607527-7607546 None:intergenic 35.0%
TCAACAAGGTTAAAACCAAC+AGG - Chr3:7607535-7607554 None:intergenic 35.0%
! GTTTGGCTACATTTTCTCTT+TGG - Chr3:7607423-7607442 None:intergenic 35.0%
ATCTTGGCTACTTCTAGCAA+TGG + Chr3:7607598-7607617 MsG0380011896.01.T01:CDS 40.0%
CTTGTGATAAGCTTAAGCAC+TGG + Chr3:7607358-7607377 MsG0380011896.01.T01:CDS 40.0%
!!! ACCTTGTTGATGTTTTGGCT+TGG + Chr3:7607545-7607564 MsG0380011896.01.T01:CDS 40.0%
!!! CCTTGTTGATGTTTTGGCTT+GGG + Chr3:7607546-7607565 MsG0380011896.01.T01:CDS 40.0%
!!! CTTGTTGATGTTTTGGCTTG+GGG + Chr3:7607547-7607566 MsG0380011896.01.T01:CDS 40.0%
CAAGGCTTACCTGAGCAAAA+TGG + Chr3:7607442-7607461 MsG0380011896.01.T01:CDS 45.0%
CCCAAGCCAAAACATCAACA+AGG - Chr3:7607549-7607568 None:intergenic 45.0%
! TGTGTCACACCATTTTGCTC+AGG - Chr3:7607454-7607473 None:intergenic 45.0%
!! TTGCTCAGGTAAGCCTTGTT+TGG - Chr3:7607440-7607459 None:intergenic 45.0%
!!! GTTTTGGCTTGGGGTTCTTT+AGG + Chr3:7607556-7607575 MsG0380011896.01.T01:CDS 45.0%
AGCTGGTGACAAACGTGCTA+AGG + Chr3:7607480-7607499 MsG0380011896.01.T01:CDS 50.0%
!! GGTGTGACACACTTTGAAGC+TGG + Chr3:7607463-7607482 MsG0380011896.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 7607277 7607645 7607277 ID=MsG0380011896.01;Name=MsG0380011896.01
Chr3 mRNA 7607277 7607645 7607277 ID=MsG0380011896.01.T01;Parent=MsG0380011896.01;Name=MsG0380011896.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|122
Chr3 exon 7607277 7607645 7607277 ID=MsG0380011896.01.T01:exon:3488;Parent=MsG0380011896.01.T01
Chr3 CDS 7607277 7607645 7607277 ID=MsG0380011896.01.T01:cds;Parent=MsG0380011896.01.T01
Gene Sequence

>MsG0380011896.01.T01

ATGGTCAACAAAAGTCAACACATTCACACCAAGCAAAAGAGATTTTCAAGTGTTGTTGTCAAAGCTGAACTTAACCCTTCACTTGTGATAAGCTTAAGCACTGGTCTTTCATTGTTTTTAGGAAGGTTTGTGTTTTTCAACTTCCAAAGAGAAAATGTAGCCAAACAAGGCTTACCTGAGCAAAATGGTGTGACACACTTTGAAGCTGGTGACAAACGTGCTAAGGAATATGTTAGCTTGCTTAAATCCAATGATCCTGTTGGTTTTAACCTTGTTGATGTTTTGGCTTGGGGTTCTTTAGGTCATATTGTTGCTTACTATATCTTGGCTACTTCTAGCAATGGTTATGATCCAAGTTTCTTTGGTTGA

Protein sequence

>MsG0380011896.01.T01

MVNKSQHIHTKQKRFSSVVVKAELNPSLVISLSTGLSLFLGRFVFFNFQRENVAKQGLPEQNGVTHFEAGDKRAKEYVSLLKSNDPVGFNLVDVLAWGSLGHIVAYYILATSSNGYDPSFFG*