AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011124.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011124.01.T01 MTR_2g099110 95.192 104 5 0 1 104 349 452 3.86e-56 180
MsG0280011124.01.T01 MTR_5g015490 41.905 105 46 4 1 104 401 491 2.65e-21 87.4
MsG0280011124.01.T01 MTR_4g097080 42.857 105 46 3 1 104 413 504 2.79e-21 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011124.01.T01 AT4G37650 42.453 106 46 5 1 104 438 530 3.54e-20 84.3

Find 28 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGAGATGATCATATTTC+AGG 0.247824 2:-79839920 MsG0280011124.01.T01:CDS
AGTGTTCAAGAAGCATAATT+AGG 0.262613 2:-79840141 MsG0280011124.01.T01:CDS
AAGAAGCATAATTAGGGTTT+TGG 0.264830 2:-79840134 MsG0280011124.01.T01:CDS
GAAGGAGGAGCCAGTTGTTT+GGG 0.360276 2:-79839882 MsG0280011124.01.T01:CDS
GGAAGGAGGAGCCAGTTGTT+TGG 0.371169 2:-79839883 MsG0280011124.01.T01:CDS
CATGTTGATGCCCAAACAAC+TGG 0.384320 2:+79839872 None:intergenic
GTGTTCAAGAAGCATAATTA+GGG 0.390341 2:-79840140 MsG0280011124.01.T01:CDS
ACGTCAAAGCGTTGCTAAAG+AGG 0.462927 2:-79839973 MsG0280011124.01.T01:CDS
AACAATGGTTTGAAAGGCTT+AGG 0.484650 2:-79840039 MsG0280011124.01.T01:CDS
GAGGAAAACAATGGTTTGAA+AGG 0.495452 2:-79840045 MsG0280011124.01.T01:CDS
ATGAGAGGTTAATGTTGGAA+AGG 0.501619 2:-79840165 None:intergenic
TGGATTTAGTGATGATGTTG+TGG 0.508345 2:-79839999 MsG0280011124.01.T01:CDS
TATTGTGATAAAAGAGAGAG+AGG 0.525178 2:-79840064 MsG0280011124.01.T01:CDS
TGAGAGGTTAATGTTGGAAA+GGG 0.526258 2:-79840164 None:intergenic
TGGTCACTTGTTGTGCCTCA+AGG 0.536151 2:-79839938 MsG0280011124.01.T01:CDS
CAGTTGTTTGGGCATCAACA+TGG 0.572778 2:-79839871 MsG0280011124.01.T01:CDS
TTTGATGATGATGAAGATGA+TGG 0.595058 2:-79840097 MsG0280011124.01.T01:CDS
AAAGAGAGAGAGGAAAACAA+TGG 0.597298 2:-79840054 MsG0280011124.01.T01:CDS
TTTCAGGAATGTACCTCACA+TGG 0.601917 2:-79839904 MsG0280011124.01.T01:CDS
TAAAGAGGTATCAAGCTGGT+TGG 0.616512 2:-79839958 MsG0280011124.01.T01:CDS
AGGAATGTACCTCACATGGA+AGG 0.622436 2:-79839900 MsG0280011124.01.T01:CDS
CTGGCTCCTCCTTCCATGTG+AGG 0.635263 2:+79839891 None:intergenic
TTGCTAAAGAGGTATCAAGC+TGG 0.647082 2:-79839962 MsG0280011124.01.T01:CDS
AATGTACCTCACATGGAAGG+AGG 0.650550 2:-79839897 MsG0280011124.01.T01:CDS
GAAATATGATCATCTCCTTG+AGG 0.654963 2:+79839923 None:intergenic
GATGATGAAGATGATGGTGG+TGG 0.659497 2:-79840091 MsG0280011124.01.T01:CDS
GATGATGATGAAGATGATGG+TGG 0.660969 2:-79840094 MsG0280011124.01.T01:CDS
ACATCATCACTAAATCCACA+AGG 0.718156 2:+79840004 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TATTGTGATAAAAGAGAGAG+AGG - Chr2:79839952-79839971 MsG0280011124.01.T01:CDS 30.0%
! AGTGTTCAAGAAGCATAATT+AGG - Chr2:79839875-79839894 MsG0280011124.01.T01:CDS 30.0%
! GTGTTCAAGAAGCATAATTA+GGG - Chr2:79839876-79839895 MsG0280011124.01.T01:CDS 30.0%
!! AAGAAGCATAATTAGGGTTT+TGG - Chr2:79839882-79839901 MsG0280011124.01.T01:CDS 30.0%
!! TTTGATGATGATGAAGATGA+TGG - Chr2:79839919-79839938 MsG0280011124.01.T01:CDS 30.0%
AAAGAGAGAGAGGAAAACAA+TGG - Chr2:79839962-79839981 MsG0280011124.01.T01:CDS 35.0%
AACAATGGTTTGAAAGGCTT+AGG - Chr2:79839977-79839996 MsG0280011124.01.T01:CDS 35.0%
ACATCATCACTAAATCCACA+AGG + Chr2:79840015-79840034 None:intergenic 35.0%
CAAGGAGATGATCATATTTC+AGG - Chr2:79840096-79840115 MsG0280011124.01.T01:CDS 35.0%
GAAATATGATCATCTCCTTG+AGG + Chr2:79840096-79840115 None:intergenic 35.0%
GAGGAAAACAATGGTTTGAA+AGG - Chr2:79839971-79839990 MsG0280011124.01.T01:CDS 35.0%
! AGGAATGAATTTTCACCTTG+TGG - Chr2:79839997-79840016 MsG0280011124.01.T01:CDS 35.0%
! TGGATTTAGTGATGATGTTG+TGG - Chr2:79840017-79840036 MsG0280011124.01.T01:CDS 35.0%
TTTCAGGAATGTACCTCACA+TGG - Chr2:79840112-79840131 MsG0280011124.01.T01:CDS 40.0%
! GATGATGATGAAGATGATGG+TGG - Chr2:79839922-79839941 MsG0280011124.01.T01:CDS 40.0%
!! TAAAGAGGTATCAAGCTGGT+TGG - Chr2:79840058-79840077 MsG0280011124.01.T01:CDS 40.0%
!! TTGCTAAAGAGGTATCAAGC+TGG - Chr2:79840054-79840073 MsG0280011124.01.T01:CDS 40.0%
AATGTACCTCACATGGAAGG+AGG - Chr2:79840119-79840138 MsG0280011124.01.T01:CDS 45.0%
AGGAATGTACCTCACATGGA+AGG - Chr2:79840116-79840135 MsG0280011124.01.T01:CDS 45.0%
CAGTTGTTTGGGCATCAACA+TGG - Chr2:79840145-79840164 MsG0280011124.01.T01:CDS 45.0%
CATGTTGATGCCCAAACAAC+TGG + Chr2:79840147-79840166 None:intergenic 45.0%
!! ACGTCAAAGCGTTGCTAAAG+AGG - Chr2:79840043-79840062 MsG0280011124.01.T01:CDS 45.0%
!! GATGATGAAGATGATGGTGG+TGG - Chr2:79839925-79839944 MsG0280011124.01.T01:CDS 45.0%
GAAGGAGGAGCCAGTTGTTT+GGG - Chr2:79840134-79840153 MsG0280011124.01.T01:CDS 50.0%
!! TGGTCACTTGTTGTGCCTCA+AGG - Chr2:79840078-79840097 MsG0280011124.01.T01:CDS 50.0%
GGAAGGAGGAGCCAGTTGTT+TGG - Chr2:79840133-79840152 MsG0280011124.01.T01:CDS 55.0%
CTGGCTCCTCCTTCCATGTG+AGG + Chr2:79840128-79840147 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 79839862 79840176 79839862 ID=MsG0280011124.01;Name=MsG0280011124.01
Chr2 mRNA 79839862 79840176 79839862 ID=MsG0280011124.01.T01;Parent=MsG0280011124.01;Name=MsG0280011124.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|104
Chr2 exon 79839862 79840176 79839862 ID=MsG0280011124.01.T01:exon:12564;Parent=MsG0280011124.01.T01
Chr2 CDS 79839862 79840176 79839862 ID=MsG0280011124.01.T01:cds;Parent=MsG0280011124.01.T01
Gene Sequence

>MsG0280011124.01.T01

ATGTTGGAAAGGGAGTGTTCAAGAAGCATAATTAGGGTTTTGGCTTGTGATCATGAATTTGATGATGATGAAGATGATGGTGGTGGTGATTATTGTGATAAAAGAGAGAGAGGAAAACAATGGTTTGAAAGGCTTAGGAATGAATTTTCACCTTGTGGATTTAGTGATGATGTTGTGGATGACGTCAAAGCGTTGCTAAAGAGGTATCAAGCTGGTTGGTCACTTGTTGTGCCTCAAGGAGATGATCATATTTCAGGAATGTACCTCACATGGAAGGAGGAGCCAGTTGTTTGGGCATCAACATGGAAACCCTAG

Protein sequence

>MsG0280011124.01.T01

MLERECSRSIIRVLACDHEFDDDEDDGGGDYCDKRERGKQWFERLRNEFSPCGFSDDVVDDVKALLKRYQAGWSLVVPQGDDHISGMYLTWKEEPVVWASTWKP*