Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016172.01.T01 | XP_003601746.1 | 94.215 | 363 | 16 | 2 | 1 | 363 | 1 | 358 | 0 | 661 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047989.01 | MsG0380016172.01 | 0.808705 | 2.716561e-50 | 1.564048e-47 |
| MsG0080048198.01 | MsG0380016172.01 | 0.834294 | 3.257382e-56 | 3.849189e-53 |
| MsG0080048528.01 | MsG0380016172.01 | 0.846664 | 1.884526e-59 | 3.267730e-56 |
| MsG0080048727.01 | MsG0380016172.01 | 0.820176 | 7.876341e-53 | 6.187479e-50 |
| MsG0080048773.01 | MsG0380016172.01 | 0.814979 | 1.169021e-51 | 7.959304e-49 |
| MsG0080048777.01 | MsG0380016172.01 | 0.800841 | 1.192959e-48 | 5.611878e-46 |
| MsG0080048780.01 | MsG0380016172.01 | 0.813535 | 2.437293e-51 | 1.595899e-48 |
| MsG0080048801.01 | MsG0380016172.01 | 0.818318 | 2.086669e-52 | 1.556776e-49 |
| MsG0080048820.01 | MsG0380016172.01 | 0.849035 | 4.186438e-60 | 7.834115e-57 |
| MsG0080048845.01 | MsG0380016172.01 | 0.820901 | 5.368260e-53 | 4.302793e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016172.01.T01 | MTR_3g084950 | 94.215 | 363 | 16 | 2 | 1 | 363 | 1 | 358 | 0.0 | 661 |
| MsG0380016172.01.T01 | MTR_8g097190 | 71.304 | 345 | 87 | 3 | 22 | 363 | 29 | 364 | 0.0 | 524 |
| MsG0380016172.01.T01 | MTR_8g097190 | 62.753 | 247 | 80 | 3 | 22 | 265 | 29 | 266 | 4.12e-110 | 323 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016172.01.T01 | AT1G10830 | 76.897 | 290 | 67 | 0 | 74 | 363 | 78 | 367 | 1.31e-177 | 498 |
| MsG0380016172.01.T01 | AT1G10830 | 73.936 | 188 | 49 | 0 | 74 | 261 | 78 | 265 | 6.10e-103 | 305 |
| MsG0380016172.01.T01 | AT1G10830 | 74.545 | 110 | 28 | 0 | 74 | 183 | 78 | 187 | 1.26e-52 | 174 |
Find 87 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATACATCTATATGAAAC+TGG | 0.374022 | 3:+79977342 | MsG0380016172.01.T01:CDS |
| AAACCGCAAATATGAGAATT+AGG | 0.277606 | 3:-79976905 | None:intergenic |
| AAACTAGCAGCCTGCATAAG+AGG | 0.442374 | 3:-79978069 | None:intergenic |
| AACAAAGATGGGTGATGGGT+TGG | 0.432814 | 3:-79976004 | None:intergenic |
| AACCACTATCTCTCTCTCAC+AGG | 0.578529 | 3:+79976113 | MsG0380016172.01.T01:CDS |
| AACCTGTGAGAGAGAGATAG+TGG | 0.513143 | 3:-79976115 | None:intergenic |
| AACTAGCAGCCTGCATAAGA+GGG | 0.580769 | 3:-79978068 | None:intergenic |
| AACTCCTTGTAGAAATCCGC+AGG | 0.550585 | 3:-79977988 | None:intergenic |
| AAGAATAACCAGGCATCCAC+AGG | 0.553400 | 3:+79977371 | MsG0380016172.01.T01:CDS |
| AAGAGGGAGAGAACAAAGAT+GGG | 0.490152 | 3:-79976015 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCGCAAATATGAGAATT+AGG | - | Chr3:79976908-79976927 | None:intergenic | 30.0% | |
| AAATCAATTCTGATACTCTC+TGG | - | Chr3:79976691-79976710 | None:intergenic | 30.0% | |
| AATACTGGTGAGAAAATCAT+TGG | + | Chr3:79976954-79976973 | MsG0380016172.01.T01:CDS | 30.0% | |
| AGATTGTTTGAGTGAACTTA+TGG | - | Chr3:79976568-79976587 | None:intergenic | 30.0% | |
| AGCATTTCGCTTATATGATT+AGG | - | Chr3:79977073-79977092 | None:intergenic | 30.0% | |
| AGGATATAATGTTACTCTTG+AGG | - | Chr3:79977614-79977633 | None:intergenic | 30.0% | |
| AGTGTTAAGATTAATACCTG+TGG | - | Chr3:79977390-79977409 | None:intergenic | 30.0% | |
| ATAAAATCAGGTTTGGGTAT+TGG | - | Chr3:79976764-79976783 | None:intergenic | 30.0% | |
| CATTACCCATTCTTCAATAA+AGG | + | Chr3:79976150-79976169 | MsG0380016172.01.T01:CDS | 30.0% | |
| GAAATGTGTCTAAATAGAGA+AGG | - | Chr3:79977702-79977721 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 79975893 | 79978256 | 79975893 | ID=MsG0380016172.01;Name=MsG0380016172.01 |
| Chr3 | mRNA | 79975893 | 79978256 | 79975893 | ID=MsG0380016172.01.T01;Parent=MsG0380016172.01;Name=MsG0380016172.01.T01;_AED=0.28;_eAED=0.28;_QI=75|1|1|1|1|1|4|149|363 |
| Chr3 | exon | 79975893 | 79976366 | 79975893 | ID=MsG0380016172.01.T01:exon:13546;Parent=MsG0380016172.01.T01 |
| Chr3 | exon | 79976891 | 79977037 | 79976891 | ID=MsG0380016172.01.T01:exon:13547;Parent=MsG0380016172.01.T01 |
| Chr3 | exon | 79977183 | 79977392 | 79977183 | ID=MsG0380016172.01.T01:exon:13548;Parent=MsG0380016172.01.T01 |
| Chr3 | exon | 79977772 | 79978256 | 79977772 | ID=MsG0380016172.01.T01:exon:13549;Parent=MsG0380016172.01.T01 |
| Chr3 | five_prime_UTR | 79975893 | 79975967 | 79975893 | ID=MsG0380016172.01.T01:five_prime_utr;Parent=MsG0380016172.01.T01 |
| Chr3 | CDS | 79975968 | 79976366 | 79975968 | ID=MsG0380016172.01.T01:cds;Parent=MsG0380016172.01.T01 |
| Chr3 | CDS | 79976891 | 79977037 | 79976891 | ID=MsG0380016172.01.T01:cds;Parent=MsG0380016172.01.T01 |
| Chr3 | CDS | 79977183 | 79977392 | 79977183 | ID=MsG0380016172.01.T01:cds;Parent=MsG0380016172.01.T01 |
| Chr3 | CDS | 79977772 | 79978107 | 79977772 | ID=MsG0380016172.01.T01:cds;Parent=MsG0380016172.01.T01 |
| Chr3 | three_prime_UTR | 79978108 | 79978256 | 79978108 | ID=MsG0380016172.01.T01:three_prime_utr;Parent=MsG0380016172.01.T01 |
| Gene Sequence |
>MsG0380016172.01.T01 ATGGCTGCCTCTCTTATACTCTCTGCTTCTTTCTCTCCAACCCATCACCCATCTTTGTTCTCTCCCTCTTATTCCATTTCATATTCCAAACCAAAACCTACTTCTCAACGCTCCTCATACTTCTCATCTCCAAATTCAAATTCAAAACCACTATCTCTCTCTCACAGGTTCAACTTCAGGCTCATTACCCATTCTTCAATAAAGGAAAAAGACTCGAGCTTCGTGGGAGAAGACTCCGCAGCTTTTAGTTTAACGGAACAGAAAATAACTTCATGGATTTATTTCGCCGCAATTTTGGGTGTGGTTCTCTTCATTCTAAATGTGGTATGGATTGATAACTCAACTGGCTTTGGTAAAGCTTTTGTTGATGCTGTTTCAGGGCTCTCCGATTCTCACGAGGTGGTAATGTTGATCCTAATTCTCATATTTGCGGTTTTCCACAGTGGCATGGCTAGCCTACGGAATACTGGTGAGAAAATCATTGGTGAAAGAGCTTTTCGGGTTATTTTTGCAGGGATATCACTACCTTTAGCTGTCACTACTATTGTGTATTTTATTAATCACAGATATGATGGAATTCAACTCTGGCAGCTCCAGAGTACTCCGGGGATTCATCAACTTCTGTGGCTTTCCAATTTCATCTCTTTCTTTTTCCTATATCCTTCAACCTTTAATCTTTTAGAAATTGCAGCAGTTGACAAGCCTAAAATACATCTATATGAAACTGGCATCATAAGAATAACCAGGCATCCACAGATGGTTGGTCAGGTAATCTGGTGTCTTGCTCATACAATTTGGATTGGGAATTCTGTGGCTGTTGCAGCTTCAATTGGCTTAATTTCACATCATCTATTTGGTGCTTGGAATGGAGATAGGCGATTAGCTCAAAGACATGGAGAAGATTTTGAGATAGTTAAGCGTCGAACAAGTATTGTCCCTTTTGCAGCAATCCTTGATGGCCGTCAAAGATTACCTGCGGATTTCTACAAGGAGTTTATTAGATTACCATACTTAGTAATTACTGCATTAACACTAGGAGCTTACTTTGCACACCCTCTTATGCAGGCTGCTAGTTTCAACCTCCATTGGTAG |
| Protein sequence |
>MsG0380016172.01.T01 MAASLILSASFSPTHHPSLFSPSYSISYSKPKPTSQRSSYFSSPNSNSKPLSLSHRFNFRLITHSSIKEKDSSFVGEDSAAFSLTEQKITSWIYFAAILGVVLFILNVVWIDNSTGFGKAFVDAVSGLSDSHEVVMLILILIFAVFHSGMASLRNTGEKIIGERAFRVIFAGISLPLAVTTIVYFINHRYDGIQLWQLQSTPGIHQLLWLSNFISFFFLYPSTFNLLEIAAVDKPKIHLYETGIIRITRHPQMVGQVIWCLAHTIWIGNSVAVAASIGLISHHLFGAWNGDRRLAQRHGEDFEIVKRRTSIVPFAAILDGRQRLPADFYKEFIRLPYLVITALTLGAYFAHPLMQAASFNLHW* |
