AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039161.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039161.01.T01 MTR_7g067000 95.720 257 11 0 1 257 1 257 2.35e-178 491
MsG0780039161.01.T01 MTR_4g015010 57.540 252 94 6 1 239 14 265 7.36e-86 257
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039161.01.T01 AT3G09050 51.200 250 103 7 4 240 2 245 2.27e-75 230

Find 36 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTGTTGGTCTTTCTTA+TGG 0.167344 7:+58900960 None:intergenic
TCTGCATACTTAACTCAATT+TGG 0.207885 7:-58901547 MsG0780039161.01.T01:CDS
AATGTTTACCTCTTCTTCAT+TGG 0.306206 7:-58901120 MsG0780039161.01.T01:CDS
GTGTTTGAAGCGTGACAATT+TGG 0.310066 7:+58901605 None:intergenic
TAAAGGCTGGTAGCGGAATC+AGG 0.365890 7:-58901146 MsG0780039161.01.T01:CDS
AGCGGAGTTTGAAGTTCAAC+TGG 0.387954 7:-58901280 MsG0780039161.01.T01:CDS
TTGCGTCGCCTTCGACTGAT+TGG 0.388513 7:-58901068 MsG0780039161.01.T01:CDS
CTTGATGTTGTTCAGTTTGA+AGG 0.416715 7:+58901750 None:intergenic
TGTTTGAAGCGTGACAATTT+GGG 0.431403 7:+58901606 None:intergenic
AACGCCGAGTTTGAAAAGAT+TGG 0.433296 7:-58901514 MsG0780039161.01.T01:CDS
CAGTTTGAAGGATGGATGTA+GGG 0.450731 7:+58901762 None:intergenic
TCCGCTACCAGCCTTTAGTC+TGG 0.451103 7:+58901152 None:intergenic
TCAGTTTGAAGGATGGATGT+AGG 0.456859 7:+58901761 None:intergenic
ATTCCTCGAATTCTAGCATC+TGG 0.480236 7:+58901349 None:intergenic
TTGTGCTTATGTAAGTCCAA+TGG 0.488206 7:-58901682 MsG0780039161.01.T01:CDS
TGTGCTTATGTAAGTCCAAT+GGG 0.490101 7:-58901681 MsG0780039161.01.T01:CDS
TTTGAAGTTCAACTGGAAAA+CGG 0.495811 7:-58901273 MsG0780039161.01.T01:CDS
AAACTACTGTAAAGGTCAGC+AGG 0.526715 7:+58901097 None:intergenic
ATCTGCAGAAATTAACAGAC+AGG 0.528683 7:-58901328 MsG0780039161.01.T01:CDS
AGCTTGAGAATCAGACCCAT+TGG 0.534125 7:+58901666 None:intergenic
GAGCCAGATGCTAGAATTCG+AGG 0.536260 7:-58901352 MsG0780039161.01.T01:CDS
AATAGAATAAACTACTGTAA+AGG 0.541708 7:+58901089 None:intergenic
TAAAGATGTCACCAGACTAA+AGG 0.542082 7:-58901163 MsG0780039161.01.T01:CDS
GATTCTCAAGCTCCTCAACC+AGG 0.562806 7:-58901655 MsG0780039161.01.T01:CDS
CTGGAAAACGGCAGAAACGA+AGG 0.566060 7:-58901261 MsG0780039161.01.T01:CDS
AGAGCGTGAAAACGAATTAG+CGG 0.567801 7:-58901298 MsG0780039161.01.T01:CDS
TTAACAGATAACAGCGAACA+TGG 0.573660 7:-58901376 MsG0780039161.01.T01:intron
ACCAGACTAAAGGCTGGTAG+CGG 0.574115 7:-58901153 MsG0780039161.01.T01:CDS
ATGTTGTTCAGTTTGAAGGA+TGG 0.576324 7:+58901754 None:intergenic
TCAGCAGGCCAATGAAGAAG+AGG 0.579281 7:+58901112 None:intergenic
AAAACATGTCAGCAGACACT+AGG 0.586732 7:-58900984 MsG0780039161.01.T01:CDS
GATGTCACCAGACTAAAGGC+TGG 0.604550 7:-58901159 MsG0780039161.01.T01:CDS
CATCTAGATATTCTGTAAGA+CGG 0.624579 7:+58901575 None:intergenic
TGATAAAGCAAAAGCCAAAG+AGG 0.625476 7:-58901199 MsG0780039161.01.T01:CDS
TGGAAAACGGCAGAAACGAA+GGG 0.643626 7:-58901260 MsG0780039161.01.T01:CDS
TAAAGCAAAAGCCAAAGAGG+AGG 0.659588 7:-58901196 MsG0780039161.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATTTACATTTTACA+TGG + Chr7:58901302-58901321 None:intergenic 10.0%
!! AAATACAAAGTGAATATAAG+AGG + Chr7:58901329-58901348 None:intergenic 20.0%
!! AATAGAATAAACTACTGTAA+AGG + Chr7:58901637-58901656 None:intergenic 20.0%
!!! TCTTGTTTTTTTGTATTTCC+TGG + Chr7:58901089-58901108 None:intergenic 25.0%
!!! TTTGTTTTCTTCTTCATTGT+TGG + Chr7:58901778-58901797 None:intergenic 25.0%
AATGTTTACCTCTTCTTCAT+TGG - Chr7:58901603-58901622 MsG0780039161.01.T01:CDS 30.0%
CATCTAGATATTCTGTAAGA+CGG + Chr7:58901151-58901170 None:intergenic 30.0%
TCTGCATACTTAACTCAATT+TGG - Chr7:58901176-58901195 MsG0780039161.01.T01:CDS 30.0%
! CTTTAAGAACCTCAGAAAAA+AGG - Chr7:58901491-58901510 MsG0780039161.01.T01:CDS 30.0%
! TTCTCCAATCTTTTCAAACT+CGG + Chr7:58901216-58901235 None:intergenic 30.0%
! TTTGAAGTTCAACTGGAAAA+CGG - Chr7:58901450-58901469 MsG0780039161.01.T01:intron 30.0%
!! TTCATTGTTGGTCTTTCTTA+TGG + Chr7:58901766-58901785 None:intergenic 30.0%
!!! TTTTTTGTATTTCCTGGTTG+AGG + Chr7:58901083-58901102 None:intergenic 30.0%
ATCTGCAGAAATTAACAGAC+AGG - Chr7:58901395-58901414 MsG0780039161.01.T01:intron 35.0%
ATGTTGTTCAGTTTGAAGGA+TGG + Chr7:58900972-58900991 None:intergenic 35.0%
CTTGATGTTGTTCAGTTTGA+AGG + Chr7:58900976-58900995 None:intergenic 35.0%
TAAAGATGTCACCAGACTAA+AGG - Chr7:58901560-58901579 MsG0780039161.01.T01:CDS 35.0%
TGATAAAGCAAAAGCCAAAG+AGG - Chr7:58901524-58901543 MsG0780039161.01.T01:CDS 35.0%
TGTGCTTATGTAAGTCCAAT+GGG - Chr7:58901042-58901061 MsG0780039161.01.T01:CDS 35.0%
TTAACAGATAACAGCGAACA+TGG - Chr7:58901347-58901366 MsG0780039161.01.T01:CDS 35.0%
TTGTGCTTATGTAAGTCCAA+TGG - Chr7:58901041-58901060 MsG0780039161.01.T01:CDS 35.0%
! GCTTTTGCTTTATCAACAAC+AGG + Chr7:58901519-58901538 None:intergenic 35.0%
! TGGAGAAAAGTTGCAGTTTT+TGG - Chr7:58901675-58901694 MsG0780039161.01.T01:CDS 35.0%
! TGTTGTAAAGTCTTTTGCAG+TGG + Chr7:58901007-58901026 None:intergenic 35.0%
! TGTTTGAAGCGTGACAATTT+GGG + Chr7:58901120-58901139 None:intergenic 35.0%
!!! AATTTTTTCTGCCTCCTCTT+TGG + Chr7:58901541-58901560 None:intergenic 35.0%
!!! AGTTTTTGGAGCAATTCTTG+TGG - Chr7:58901689-58901708 MsG0780039161.01.T01:CDS 35.0%
AAAACATGTCAGCAGACACT+AGG - Chr7:58901739-58901758 MsG0780039161.01.T01:CDS 40.0%
AAACTACTGTAAAGGTCAGC+AGG + Chr7:58901629-58901648 None:intergenic 40.0%
AACGCCGAGTTTGAAAAGAT+TGG - Chr7:58901209-58901228 MsG0780039161.01.T01:CDS 40.0%
AGAGCGTGAAAACGAATTAG+CGG - Chr7:58901425-58901444 MsG0780039161.01.T01:intron 40.0%
ATTCCTCGAATTCTAGCATC+TGG + Chr7:58901377-58901396 None:intergenic 40.0%
CAGTTTGAAGGATGGATGTA+GGG + Chr7:58900964-58900983 None:intergenic 40.0%
TAAAGCAAAAGCCAAAGAGG+AGG - Chr7:58901527-58901546 MsG0780039161.01.T01:CDS 40.0%
TCAGTTTGAAGGATGGATGT+AGG + Chr7:58900965-58900984 None:intergenic 40.0%
! ACTTTTCTCCAATCAGTCGA+AGG + Chr7:58901666-58901685 None:intergenic 40.0%
! GTGTTTGAAGCGTGACAATT+TGG + Chr7:58901121-58901140 None:intergenic 40.0%
! TTACATGGTTAGCTCGAAAG+TGG + Chr7:58901287-58901306 None:intergenic 40.0%
!!! CAACAGGTACCTTTTTTCTG+AGG + Chr7:58901503-58901522 None:intergenic 40.0%
AAGTGGAAGCAAGCATACTC+TGG + Chr7:58901270-58901289 None:intergenic 45.0%
AGCTTGAGAATCAGACCCAT+TGG + Chr7:58901060-58901079 None:intergenic 45.0%
TGGAAAACGGCAGAAACGAA+GGG - Chr7:58901463-58901482 MsG0780039161.01.T01:intron 45.0%
! AGCGGAGTTTGAAGTTCAAC+TGG - Chr7:58901443-58901462 MsG0780039161.01.T01:intron 45.0%
ACCAGACTAAAGGCTGGTAG+CGG - Chr7:58901570-58901589 MsG0780039161.01.T01:CDS 50.0%
CTGGAAAACGGCAGAAACGA+AGG - Chr7:58901462-58901481 MsG0780039161.01.T01:intron 50.0%
GAGCCAGATGCTAGAATTCG+AGG - Chr7:58901371-58901390 MsG0780039161.01.T01:CDS 50.0%
GATGTCACCAGACTAAAGGC+TGG - Chr7:58901564-58901583 MsG0780039161.01.T01:CDS 50.0%
GATTCTCAAGCTCCTCAACC+AGG - Chr7:58901068-58901087 MsG0780039161.01.T01:CDS 50.0%
TCAGCAGGCCAATGAAGAAG+AGG + Chr7:58901614-58901633 None:intergenic 50.0%
!! TAAAGGCTGGTAGCGGAATC+AGG - Chr7:58901577-58901596 MsG0780039161.01.T01:CDS 50.0%
TCCGCTACCAGCCTTTAGTC+TGG + Chr7:58901574-58901593 None:intergenic 55.0%
TTGCGTCGCCTTCGACTGAT+TGG - Chr7:58901655-58901674 MsG0780039161.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 58900945 58901800 58900945 ID=MsG0780039161.01;Name=MsG0780039161.01
Chr7 mRNA 58900945 58901800 58900945 ID=MsG0780039161.01.T01;Parent=MsG0780039161.01;Name=MsG0780039161.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|1|1|2|0|257
Chr7 exon 58901474 58901800 58901474 ID=MsG0780039161.01.T01:exon:6887;Parent=MsG0780039161.01.T01
Chr7 exon 58900945 58901391 58900945 ID=MsG0780039161.01.T01:exon:6886;Parent=MsG0780039161.01.T01
Chr7 CDS 58901474 58901800 58901474 ID=MsG0780039161.01.T01:cds;Parent=MsG0780039161.01.T01
Chr7 CDS 58900945 58901391 58900945 ID=MsG0780039161.01.T01:cds;Parent=MsG0780039161.01.T01
Gene Sequence

>MsG0780039161.01.T01

ATGATTGCTCTGAAATCCCTACATCCATCCTTCAAACTGAACAACATCAAGTGTAATATCCACTGCAAAAGACTTTACAACACAAGAAGTTATTCATTGTGCTTATGTAAGTCCAATGGGTCTGATTCTCAAGCTCCTCAACCAGGAAATACAAAAAAACAAGAACTTCTAGCCCAAATTGTCACGCTTCAAACACGAAAAGTCCGTCTTACAGAATATCTAGATGAGAGATCTGCATACTTAACTCAATTTGGTGAAAAAGTAAACGCCGAGTTTGAAAAGATTGGAGAAGATGCACTCAAAGAATTAGATGAAGCTAGTGCCAGAATAACAGCGAACATGGAGAGCCAGATGCTAGAATTCGAGGAATCTGCAGAAATTAACAGACAGGAGATTCAAGAGCGTGAAAACGAATTAGCGGAGTTTGAAGTTCAACTGGAAAACGGCAGAAACGAAGGGCTATTCTTTAAGAACCTCAGAAAAAAGGTACCTGTTGTTGATAAAGCAAAAGCCAAAGAGGAGGCAGAAAAAATTAAAGATGTCACCAGACTAAAGGCTGGTAGCGGAATCAGGAAAAATGTTTACCTCTTCTTCATTGGCCTGCTGACCTTTACAGTAGTTTATTCTATTGCGTCGCCTTCGACTGATTGGAGAAAAGTTGCAGTTTTTGGAGCAATTCTTGTGGCTTTGACTTCTCAGTTCGTCTATGAACAAAACATGTCAGCAGACACTAGGACCATAAGAAAGACCAACAATGAAGAAGAAAACAAATGA

Protein sequence

>MsG0780039161.01.T01

MIALKSLHPSFKLNNIKCNIHCKRLYNTRSYSLCLCKSNGSDSQAPQPGNTKKQELLAQIVTLQTRKVRLTEYLDERSAYLTQFGEKVNAEFEKIGEDALKELDEASARITANMESQMLEFEESAEINRQEIQERENELAEFEVQLENGRNEGLFFKNLRKKVPVVDKAKAKEEAEKIKDVTRLKAGSGIRKNVYLFFIGLLTFTVVYSIASPSTDWRKVAVFGAILVALTSQFVYEQNMSADTRTIRKTNNEEENK*