AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045648.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045648.01.T01 MTR_8g075960 96.721 183 6 0 1 183 1 183 2.52e-130 363
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045648.01.T01 AT2G17972 48.529 136 56 3 46 181 37 158 1.37e-36 125

Find 52 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCAATCTTCATAATGAT+TGG 0.317068 8:+62152033 MsG0880045648.01.T01:CDS
CGCCGCAGCCTTAGTTCAAC+TGG 0.318125 8:-62151832 None:intergenic
CTCATGTCCTCCCATTTCTT+AGG 0.342760 8:-62151937 None:intergenic
GGTGCTACTGCTGCTGCTGC+AGG 0.353627 8:-62151746 None:intergenic
ATGGATAGCGTTTAGGTTTG+TGG 0.358027 8:+62152059 MsG0880045648.01.T01:CDS
TTGGGCTATGGATAGCGTTT+AGG 0.362432 8:+62152052 MsG0880045648.01.T01:CDS
CAAAGGCTGCTCCTAAGAAA+TGG 0.371169 8:+62151926 MsG0880045648.01.T01:CDS
TTAGGAGCAGCCTTTGCCTT+GGG 0.384532 8:-62151919 None:intergenic
TGGATAGCGTTTAGGTTTGT+GGG 0.391731 8:+62152060 MsG0880045648.01.T01:CDS
ACGTCTAGTTAGAATAATAT+TGG 0.400280 8:-62151644 None:intergenic
ACTGCTGCTGCTGCAGGTGA+TGG 0.408197 8:-62151740 None:intergenic
AACTGGTTGTGTCTGGTTGA+TGG 0.417616 8:-62151679 None:intergenic
CTTCAATCTTCATAATGATT+GGG 0.425261 8:+62152034 MsG0880045648.01.T01:CDS
CTTAGGAGCAGCCTTTGCCT+TGG 0.425440 8:-62151920 None:intergenic
TGCACTTAATATTTATCAGT+TGG 0.425443 8:+62152086 MsG0880045648.01.T01:CDS
GCCAGAAAACAGAGAGAGAA+TGG 0.442782 8:+62151871 MsG0880045648.01.T01:CDS
ACTGGTTGTGTCTGGTTGAT+GGG 0.451612 8:-62151678 None:intergenic
GAGCTTTATGTAGGAGAGAA+AGG 0.453819 8:+62151979 MsG0880045648.01.T01:CDS
CTTAGTTCAACTGGTTCAGC+TGG 0.457887 8:-62151823 None:intergenic
TGAAGGTGAGTTTGAAGAGA+GGG 0.458101 8:-62151617 None:intergenic
TCAACTGGTTCAGCTGGTGT+TGG 0.466879 8:-62151817 None:intergenic
GTGAAGGTGAGTTTGAAGAG+AGG 0.477627 8:-62151618 None:intergenic
GGTTCAGCTGGTGTTGGAGT+TGG 0.493855 8:-62151811 None:intergenic
ATCGTCGTTGGTGTTTGTGT+TGG 0.496861 8:-62151767 None:intergenic
AAGGCACTTGAGCTTTATGT+AGG 0.500462 8:+62151970 MsG0880045648.01.T01:CDS
TGAGTTTGAAGAGAGGGAGG+TGG 0.502944 8:-62151611 None:intergenic
TGAGAGGAACTGGTTGTGTC+TGG 0.505814 8:-62151686 None:intergenic
TAGGTTGAAATGAGAGGAAC+TGG 0.508744 8:-62151696 None:intergenic
CAGCTGAACCAGTTGAACTA+AGG 0.513049 8:+62151824 MsG0880045648.01.T01:CDS
AAAGGCTGCTCCTAAGAAAT+GGG 0.520681 8:+62151927 MsG0880045648.01.T01:CDS
TGCTGCTGCAGGTGATGGAT+TGG 0.523749 8:-62151735 None:intergenic
AGGTGAGTTTGAAGAGAGGG+AGG 0.534562 8:-62151614 None:intergenic
AACTGATAAATATTAAGTGC+AGG 0.538359 8:-62152084 None:intergenic
GATCCCTTCAGTACATCCTC+AGG 0.542081 8:-62152126 None:intergenic
TCTTCATAATGATTGGGCTA+TGG 0.548140 8:+62152040 MsG0880045648.01.T01:CDS
GTTGTGTCTGGTTGATGGGA+GGG 0.554560 8:-62151674 None:intergenic
GTCACCTGAGGATGTACTGA+AGG 0.556978 8:+62152122 MsG0880045648.01.T01:CDS
GGACATGAGTTTCACTGAGA+AGG 0.582048 8:+62151951 MsG0880045648.01.T01:CDS
AAGTGGTAGGTTGAAATGAG+AGG 0.590851 8:-62151702 None:intergenic
AGAATAATATTGGTGAGTGA+AGG 0.591713 8:-62151634 None:intergenic
GGTTGTGTCTGGTTGATGGG+AGG 0.602056 8:-62151675 None:intergenic
CATACAGCAACCCAAGGCAA+AGG 0.612470 8:+62151909 MsG0880045648.01.T01:CDS
TGGAAAGTGAGGGAAGTGGT+AGG 0.621474 8:-62151715 None:intergenic
AACCAGTTGAACTAAGGCTG+CGG 0.639608 8:+62151830 MsG0880045648.01.T01:CDS
ACATCCTCAGGTGACAGTAG+AGG 0.640948 8:-62152114 None:intergenic
GGTGATGGATTGGAAAGTGA+GGG 0.642731 8:-62151725 None:intergenic
TCACCTGAGGATGTACTGAA+GGG 0.654909 8:+62152123 MsG0880045648.01.T01:CDS
GGCTGCTCCTAAGAAATGGG+AGG 0.656833 8:+62151930 MsG0880045648.01.T01:CDS
AAATAGCATACAGCAACCCA+AGG 0.666926 8:+62151903 MsG0880045648.01.T01:CDS
AGGTGATGGATTGGAAAGTG+AGG 0.686558 8:-62151726 None:intergenic
GGATTGGAAAGTGAGGGAAG+TGG 0.688837 8:-62151719 None:intergenic
TCAACCTCTACTGTCACCTG+AGG 0.718719 8:+62152110 MsG0880045648.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTGATAAATATTAAGTGC+AGG - Chr8:62152087-62152106 None:intergenic 25.0%
! ACGTCTAGTTAGAATAATAT+TGG - Chr8:62151647-62151666 None:intergenic 25.0%
! CTTCAATCTTCATAATGATT+GGG + Chr8:62152034-62152053 MsG0880045648.01.T01:CDS 25.0%
! TGCACTTAATATTTATCAGT+TGG + Chr8:62152086-62152105 MsG0880045648.01.T01:CDS 25.0%
AGAATAATATTGGTGAGTGA+AGG - Chr8:62151637-62151656 None:intergenic 30.0%
GCTTCAATCTTCATAATGAT+TGG + Chr8:62152033-62152052 MsG0880045648.01.T01:CDS 30.0%
TCTTCATAATGATTGGGCTA+TGG + Chr8:62152040-62152059 MsG0880045648.01.T01:CDS 35.0%
!! TGAATCTTTTTCATCGTCGT+TGG - Chr8:62151782-62151801 None:intergenic 35.0%
AAAGGCTGCTCCTAAGAAAT+GGG + Chr8:62151927-62151946 MsG0880045648.01.T01:CDS 40.0%
AAATAGCATACAGCAACCCA+AGG + Chr8:62151903-62151922 MsG0880045648.01.T01:CDS 40.0%
TGAAGGTGAGTTTGAAGAGA+GGG - Chr8:62151620-62151639 None:intergenic 40.0%
! AAGGCACTTGAGCTTTATGT+AGG + Chr8:62151970-62151989 MsG0880045648.01.T01:CDS 40.0%
! AAGTGGTAGGTTGAAATGAG+AGG - Chr8:62151705-62151724 None:intergenic 40.0%
! ATGGATAGCGTTTAGGTTTG+TGG + Chr8:62152059-62152078 MsG0880045648.01.T01:CDS 40.0%
! GAGCTTTATGTAGGAGAGAA+AGG + Chr8:62151979-62151998 MsG0880045648.01.T01:CDS 40.0%
! TAGGTTGAAATGAGAGGAAC+TGG - Chr8:62151699-62151718 None:intergenic 40.0%
! TCCATTCTCTCTCTGTTTTC+TGG - Chr8:62151875-62151894 None:intergenic 40.0%
! TGGATAGCGTTTAGGTTTGT+GGG + Chr8:62152060-62152079 MsG0880045648.01.T01:CDS 40.0%
!!! GAGAGAAAGGTGCTCTTTTT+TGG + Chr8:62151992-62152011 MsG0880045648.01.T01:CDS 40.0%
AACCAGTTGAACTAAGGCTG+CGG + Chr8:62151830-62151849 MsG0880045648.01.T01:CDS 45.0%
AACTGGTTGTGTCTGGTTGA+TGG - Chr8:62151682-62151701 None:intergenic 45.0%
ACTGGTTGTGTCTGGTTGAT+GGG - Chr8:62151681-62151700 None:intergenic 45.0%
CAAAGGCTGCTCCTAAGAAA+TGG + Chr8:62151926-62151945 MsG0880045648.01.T01:CDS 45.0%
CAGCTGAACCAGTTGAACTA+AGG + Chr8:62151824-62151843 MsG0880045648.01.T01:CDS 45.0%
CTCATGTCCTCCCATTTCTT+AGG - Chr8:62151940-62151959 None:intergenic 45.0%
GCCAGAAAACAGAGAGAGAA+TGG + Chr8:62151871-62151890 MsG0880045648.01.T01:CDS 45.0%
GGACATGAGTTTCACTGAGA+AGG + Chr8:62151951-62151970 MsG0880045648.01.T01:CDS 45.0%
GTGAAGGTGAGTTTGAAGAG+AGG - Chr8:62151621-62151640 None:intergenic 45.0%
TCACCTGAGGATGTACTGAA+GGG + Chr8:62152123-62152142 MsG0880045648.01.T01:CDS 45.0%
TTGGGCTATGGATAGCGTTT+AGG + Chr8:62152052-62152071 MsG0880045648.01.T01:CDS 45.0%
! ATCGTCGTTGGTGTTTGTGT+TGG - Chr8:62151770-62151789 None:intergenic 45.0%
! CTTAGTTCAACTGGTTCAGC+TGG - Chr8:62151826-62151845 None:intergenic 45.0%
!! AGGTGATGGATTGGAAAGTG+AGG - Chr8:62151729-62151748 None:intergenic 45.0%
!! GGTGATGGATTGGAAAGTGA+GGG - Chr8:62151728-62151747 None:intergenic 45.0%
ACATCCTCAGGTGACAGTAG+AGG - Chr8:62152117-62152136 None:intergenic 50.0%
AGGTGAGTTTGAAGAGAGGG+AGG - Chr8:62151617-62151636 None:intergenic 50.0%
CATACAGCAACCCAAGGCAA+AGG + Chr8:62151909-62151928 MsG0880045648.01.T01:CDS 50.0%
GATCCCTTCAGTACATCCTC+AGG - Chr8:62152129-62152148 None:intergenic 50.0%
GGATTGGAAAGTGAGGGAAG+TGG - Chr8:62151722-62151741 None:intergenic 50.0%
GTCACCTGAGGATGTACTGA+AGG + Chr8:62152122-62152141 MsG0880045648.01.T01:CDS 50.0%
TCAACCTCTACTGTCACCTG+AGG + Chr8:62152110-62152129 MsG0880045648.01.T01:CDS 50.0%
TGAGAGGAACTGGTTGTGTC+TGG - Chr8:62151689-62151708 None:intergenic 50.0%
TGAGTTTGAAGAGAGGGAGG+TGG - Chr8:62151614-62151633 None:intergenic 50.0%
TGGAAAGTGAGGGAAGTGGT+AGG - Chr8:62151718-62151737 None:intergenic 50.0%
TTAGGAGCAGCCTTTGCCTT+GGG - Chr8:62151922-62151941 None:intergenic 50.0%
! TCAACTGGTTCAGCTGGTGT+TGG - Chr8:62151820-62151839 None:intergenic 50.0%
!! GTTGTGTCTGGTTGATGGGA+GGG - Chr8:62151677-62151696 None:intergenic 50.0%
CTTAGGAGCAGCCTTTGCCT+TGG - Chr8:62151923-62151942 None:intergenic 55.0%
GGCTGCTCCTAAGAAATGGG+AGG + Chr8:62151930-62151949 MsG0880045648.01.T01:CDS 55.0%
! GGTTCAGCTGGTGTTGGAGT+TGG - Chr8:62151814-62151833 None:intergenic 55.0%
!! GGTTGTGTCTGGTTGATGGG+AGG - Chr8:62151678-62151697 None:intergenic 55.0%
!! TGCTGCTGCAGGTGATGGAT+TGG - Chr8:62151738-62151757 None:intergenic 55.0%
ACTGCTGCTGCTGCAGGTGA+TGG - Chr8:62151743-62151762 None:intergenic 60.0%
CGCCGCAGCCTTAGTTCAAC+TGG - Chr8:62151835-62151854 None:intergenic 60.0%
!! GGTGCTACTGCTGCTGCTGC+AGG - Chr8:62151749-62151768 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 62151604 62152155 62151604 ID=MsG0880045648.01;Name=MsG0880045648.01
Chr8 mRNA 62151604 62152155 62151604 ID=MsG0880045648.01.T01;Parent=MsG0880045648.01;Name=MsG0880045648.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|183
Chr8 exon 62151604 62152155 62151604 ID=MsG0880045648.01.T01:exon:23755;Parent=MsG0880045648.01.T01
Chr8 CDS 62151604 62152155 62151604 ID=MsG0880045648.01.T01:cds;Parent=MsG0880045648.01.T01
Gene Sequence

>MsG0880045648.01.T01

ATGGGAACCACCTCCCTCTCTTCAAACTCACCTTCACTCACCAATATTATTCTAACTAGACGTTCAAGTTCCCTCCCATCAACCAGACACAACCAGTTCCTCTCATTTCAACCTACCACTTCCCTCACTTTCCAATCCATCACCTGCAGCAGCAGCAGTAGCACCAACACAAACACCAACGACGATGAAAAAGATTCAGTTCAAGCACCAACTCCAACACCAGCTGAACCAGTTGAACTAAGGCTGCGGCGAAGATCAAAAAGACAAGCCAGAAAACAGAGAGAGAATGGAACTCTCACAAATAGCATACAGCAACCCAAGGCAAAGGCTGCTCCTAAGAAATGGGAGGACATGAGTTTCACTGAGAAGGCACTTGAGCTTTATGTAGGAGAGAAAGGTGCTCTTTTTTGGCTTAATAAATTTGCATATGCTTCAATCTTCATAATGATTGGGCTATGGATAGCGTTTAGGTTTGTGGGTCCTGCACTTAATATTTATCAGTTGGATCAACCTCTACTGTCACCTGAGGATGTACTGAAGGGATCTTCCTGA

Protein sequence

>MsG0880045648.01.T01

MGTTSLSSNSPSLTNIILTRRSSSLPSTRHNQFLSFQPTTSLTFQSITCSSSSSTNTNTNDDEKDSVQAPTPTPAEPVELRLRRRSKRQARKQRENGTLTNSIQQPKAKAAPKKWEDMSFTEKALELYVGEKGALFWLNKFAYASIFIMIGLWIAFRFVGPALNIYQLDQPLLSPEDVLKGSS*